Research Topics
| CLINT MAKINOSummaryAffiliation: Harvard University Country: USA Publications
Research Grants
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Detail Information
Publications
Rhodopsin expression level affects rod outer segment morphology and photoresponse kineticsClint L Makino
Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS ONE 7:e37832. 2012..The finding that transgenic mice overexpressing rhodopsin have enlarged disks lacking an incisure prompted us to test whether lowered rhodopsin levels constrain disk assembly...- Binding of more than one retinoid to visual opsinsClint L Makino
Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, MA, USA
Biophys J 99:2366-73. 2010..Therefore, both blue- and green-sensitive rod rhodopsins have at least one allosteric binding site for retinoid, but β-ionone binds to the latter type of rhodopsin with low affinity and low efficacy... 
Effects of low AIPL1 expression on phototransduction in rodsClint L Makino
Howe Laboratory, Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston 02114, USA
Invest Ophthalmol Vis Sci 47:2185-94. 2006..To investigate the impact of aryl hydrocarbon receptor-interacting protein-like (AIPL)-1 on photoreception in rods...- Spectral tuning in salamander visual pigments studied with dihydroretinal chromophoresC L Makino
Department of Ophthalmology, Harvard Medical School, and the Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA
Biophys J 77:1024-35. 1999..Thus an interaction between opsin and the chromophoric ring shifts the spectral maxima of the red and blue cone pigments, but not that of the red rod pigment... - Recoverin regulates light-dependent phosphodiesterase activity in retinal rodsClint L Makino
Department of Ophthalmology, Harvard Medical School and the Massachusetts Eye and Ear Infirmary, 243 Charles Street, Boston, MA 02114, USA
J Gen Physiol 123:729-41. 2004..We infer that Ca2+-recoverin potentiates light-triggered phosphodiesterase activity, probably by effectively prolonging the catalytic activity of photoexcited rhodopsin... - Piecing together the timetable for visual transduction with transgenic animalsClint L Makino
Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, 243 Charles Street, Boston, MA 02114, USA
Curr Opin Neurobiol 13:404-12. 2003..The timetable for all of these molecular events determines the amplitude, kinetics and reproducibility of the photoresponse... - A role for GCAP2 in regulating the photoresponse. Guanylyl cyclase activation and rod electrophysiology in GUCA1B knock-out miceClint L Makino
Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, Massachusetts 02114, USA
J Biol Chem 283:29135-43. 2008..Therefore, GCAP2 regulation of guanylyl cyclase activity quickens the recovery of flash and step responses and adjusts the operating range of rods to higher intensities of ambient illumination... - The time course of light adaptation in vertebrate retinal rodsPeter D Calvert
Department of Ophthalmology, Harvard Medical School and the Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA
Adv Exp Med Biol 514:37-60. 2002..Although the mechanism(s) of the slow phase is not yet known, it appears to include further regulation of the lifetime of photoactivated rhodopsin... - Two temporal phases of light adaptation in retinal rodsPeter D Calvert
Department of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA
J Gen Physiol 119:129-45. 2002..Other possible mechanisms are discussed... - Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptorsXiao Hong Wen
Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, Massachusetts, USA
Biophys J 96:939-50. 2009..That feature, as well as an increase in cytosolic volume, was responsible for delaying the onset of the photoresponse and for attenuating its amplification... - Enhanced shutoff of phototransduction in transgenic mice expressing palmitoylation-deficient rhodopsinZhongyan Wang
Molecular Cardiology Research Institute, Tufts New England Medical Center, Boston, Massachusetts 02111, USA
J Biol Chem 280:24293-300. 2005..Since quench of rhodopsin begins with its phosphorylation, these results suggest that palmitoylation may modulate rod photoreceptor sensitivity by permitting rhodopsin to remain active for a longer period... 
Cyclic nucleotide-gated ion channels in rod photoreceptors are protected from retinoid inhibitionQuanhua He
Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA 02114, USA
J Gen Physiol 128:473-85. 2006..Exceptionally high retinoid levels, e.g., associated with some retinal degenerations, could overcome the buffer, however, and impair sensitivity or delay the recovery after exposure to bright light...- AIPL1, the protein that is defective in Leber congenital amaurosis, is essential for the biosynthesis of retinal rod cGMP phosphodiesteraseXiaoqing Liu
Berman Gund Laboratory for the Study of Retinal Degenerations, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 101:13903-8. 2004..We suggest that AIPL1 is a specialized chaperone required for rod PDE biosynthesis. Thus loss of AIPL1 would result in a condition that phenocopies retinal degenerations in the rd mouse and in a subgroup of human patients... - Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminalsJun Yang
The Berman Gund Laboratory for Study of Retinal Degenerations, Department of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA
Hum Mol Genet 16:1017-29. 2007..We propose that Mpp4 organizes a presynaptic protein complex that includes PMCAs and has a role in modulating Ca(2+) homeostasis and synaptic transmission in rod photoreceptors... - Increased light exposure alleviates one form of photoreceptor degeneration marked by elevated calcium in the darkXiaoqing Liu
Berman Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States of America
PLoS ONE 4:e8438. 2009..We determined whether prolonged light exposure, which lowers cGMP levels through activation of phototransduction, might protect photoreceptors in a line of transgenic mice carrying the GCAP1-Y99C... - The ciliary rootlet maintains long-term stability of sensory ciliaJun Yang
The Berman Gund Laboratory for the Study of Retinal Degenerations, Howe Laboratory, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA
Mol Cell Biol 25:4129-37. 2005..Inasmuch as photoreceptors elaborate an exceptionally enlarged sensory cilium, they are especially dependent on the rootlet for structural integrity and long-term survival... - The Y99C mutation in guanylyl cyclase-activating protein 1 increases intracellular Ca2+ and causes photoreceptor degeneration in transgenic miceElena V Olshevskaya
Hafter Research Laboratories, Pennsylvania College of Optometry, Elkins Park, Pennsylvania 19027, USA
J Neurosci 24:6078-85. 2004..These results provide the first direct evidence that a mutation linked to congenital blindness increases Ca2+ in the outer segment, which may trigger the apoptotic process...
Research Grants
- Multiple Ligand Binding Sites of Rod and Cone OpsinsCLINT MAKINO; Fiscal Year: 2009..Therefore, this proposal will be highly significant to our understanding of the structure and function of visual opsins and opens new directions for vision research. ..
- Function of the visual pigment in situCLINT MAKINO; Fiscal Year: 2005..abstract_text> ..
- Light Adaptation in Vertebrate Rod PhotoreceptorsCLINT MAKINO; Fiscal Year: 2005..Aberrant responses occur with higher frequency in some human retinal diseases and severely limit the intensity range of scotopic vision. ..
- Multiple Ligand Binding Sites of Rod and Cone OpsinsClint L Makino; Fiscal Year: 2010..Therefore, this proposal will be highly significant to our understanding of the structure and function of visual opsins and opens new directions for vision research. ..