Research Topics
Genomes and Genes
| Francis W LuscinskasSummaryAffiliation: Harvard University Country: USA Publications
| Collaborators
|
Detail Information
Publications
Dendritic cell expression of the transcription factor T-bet regulates mast cell progenitor homing to mucosal tissuePilar Alcaide
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
J Exp Med 204:431-9. 2007..Therefore, remarkably, T-bet expression by DCs indirectly controls MCp homing to mucosal tissues...
C-C and C-X-C chemokines trigger firm adhesion of monocytes to vascular endothelium under flow conditionsF W Luscinskas
Department of Pathology, Brigham and Women s Hospital, Boston, Massachusett 02115, USA
Ann N Y Acad Sci 902:288-93. 2000....- Leukocyte transendothelial migration: a junctional affairFrancis W Luscinskas
Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
Semin Immunol 14:105-13. 2002..This article will focus on the endothelial-dependent processes that coordinate transmigrations in peripheral vasculature during the inflammatory response... - The role of endothelial cell lateral junctions during leukocyte traffickingFrancis W Luscinskas
Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
Immunol Rev 186:57-67. 2002..This article reviews the endothelial-dependent mechanisms that coordinate TEM in peripheral vasculature and highlights the role of certain lateral junctional proteins and protein complexes... - p120-Catenin regulates leukocyte transmigration through an effect on VE-cadherin phosphorylationPilar Alcaide
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
Blood 112:2770-9. 2008.... - Endothelial CD47 promotes vascular endothelial-cadherin tyrosine phosphorylation and participates in T cell recruitment at sites of inflammation in vivoVeronica Azcutia
Department of Pathology, Center for Excellence in Vascular Biology, Boston, MA 02115, USA
J Immunol 189:2553-62. 2012..These findings suggest that engagement of endothelial CD47 by its ligands triggers outside-in signals in endothelium that facilitate leukocyte TEM... - Endothelial cell cortactin coordinates intercellular adhesion molecule-1 clustering and actin cytoskeleton remodeling during polymorphonuclear leukocyte adhesion and transmigrationLin Yang
Center for Excellence in Vascular Biology, Departments of Pathology, Brigham and Women s Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
J Immunol 177:6440-9. 2006..Our data suggest a model in which tyrosine phosphorylation of cortactin dynamically links ICAM-1 to the actin cytoskeleton, enabling ICAM-1 to form clusters and facilitate leukocyte transmigration... - Neutrophil recruitment under shear flow: it's all about endothelial cell rings and gapsPilar Alcaide
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
Microcirculation 16:43-57. 2009..Interestingly, the signaling events that lead to neutrophil transmigration occur independently of shear flow in vitro... - Apolipoprotein CIII induces expression of vascular cell adhesion molecule-1 in vascular endothelial cells and increases adhesion of monocytic cellsAkio Kawakami
Department of Nutrition, Harvard School of Public Health, 665 Huntington Ave, Boston, MA 02115, USA
Circulation 114:681-7. 2006..Therefore, apoCIII-rich VLDL may contribute directly to atherogenesis by activating ECs and recruiting monocytes to them... - p120-Catenin prevents neutrophil transmigration independently of RhoA inhibition by impairing Src dependent VE-cadherin phosphorylationPilar Alcaide
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital, Boston, MA, USA
Am J Physiol Cell Physiol 303:C385-95. 2012..In summary, p120 overexpression inhibits neutrophil TEM independently of an effect on RhoA or Rac and instead blocks TEM by preventing VE-cad tyrosine phosphorylation and association of active Src with the VE-cad complex... - The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivoPilar Alcaide
Department of Pathology, Center for Excellence in Vascular Biology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
J Invest Dermatol 127:1964-72. 2007..These results demonstrate that both PSGL-1 and CD43 are major E-selectin ligands and are likely to be important during leukocyte recruitment in the development of inflammatory reactions... - Neutrophil transmigration under shear flow conditions in vitro is junctional adhesion molecule-C independentMonica Sircar
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
J Immunol 178:5879-87. 2007..These data suggest that JAM-C has a minimal role, if any, in PMN transmigration in this model and that ICAM-1 is the preferred endothelial-expressed ligand for PMN beta(2) integrins during transendothelial migration... - Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin mediated human T-cell adhesionHaifa Ghandour
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
Blood 110:3682-90. 2007..Thus, PLC/CalDAG-GEFI regulation of Rap1 is selectively required for chemokine- and PMA-induced LFA-1 activation in human T cells, whereas alternate PLC- and PKC-dependent mechanisms are involved in the regulation of VLA-4... - Difference in Th1 and Th17 lymphocyte adhesion to endotheliumPilar Alcaide
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
J Immunol 188:1421-30. 2012..In an air pouch model of inflammation, CCL20 triggered recruitment of Th17 but not Th1 cells. These data provide evidence that E-selectin- and ICAM-1-dependent adhesion of Th17 and Th1 cells with endothelium are quantitatively different... - ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-alpha-activated vascular endothelium under flowLin Yang
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, NRB 752P, Boston, MA 02215, USA
Blood 106:584-92. 2005..In summary, ICAM-1 promotes junctional and nonjunctional TEM across inflamed vascular endothelium via distinct cytoplasmic tail associations... - Neutrophils from MMP-9- or neutrophil elastase-deficient mice show no defect in transendothelial migration under flow in vitroJennifer R Allport
Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA
J Leukoc Biol 71:821-8. 2002..These results indicate that although neutrophil proteases may play a role in the acute inflammatory response, neutrophil elastase or MMP-9 is not essential for neutrophil transendothelial migration in this murine system... - Heterogeneity of endothelial cells from different organ sites in T-cell subset recruitmentYaw-Chyn Lim
Department of Pathology, Vascular Research Division, Brigham and Women's Hospital and Harvard Medical School, Boston Massachusetts 02115, USA
Am J Pathol 162:1591-601. 2003..Furthermore, these results provide an insight into the molecular mechanisms that may mediate T-helper cell recruitment to these organs... - Abrogation of functional selectin-ligand expression reduces migration of pathogenic CD8+ T cells into heartYi Hong Cai
Vascular Research Division, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
J Immunol 176:6568-75. 2006..These results identify a prominent role for selectin ligands in contributing to effector CD8+ T cell recruitment into the myocardium and indicate that selectin-dependent T cell recruitment is relevant to other tissues besides the skin... - Endothelial cell cortactin phosphorylation by Src contributes to polymorphonuclear leukocyte transmigration in vitroLin Yang
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
Circ Res 98:394-402. 2006..Moreover, expression of cortactin3F alone partially blocked PMN transmigration. These data suggest a model whereby tyrosine phosphorylation of cortactin by Src family kinases regulates PMN transmigration... - Elastase release by transmigrating neutrophils deactivates endothelial-bound SDF-1alpha and attenuates subsequent T lymphocyte transendothelial migrationRavi M Rao
Department of Pathology, Center for Excellence in Vascular Biology, Brigham and Women s Hospital, Harvard Medical School, 77 Ave Louis Pasteur NRB 752P, Boston, MA 02115, USA
J Exp Med 200:713-24. 2004..In summary, transmigrating neutrophils secrete localized elastase that is protected from plasma inhibitors, and thereby modulate trafficking of other leukocyte subsets by altering the endothelial-associated chemotactic activities... - Regulation of endothelial cell adhesion molecule expression by mast cells, macrophages, and neutrophilsJie Zhang
Department of Medicine, Harvard Medical School, Brigham and Women s Hospital, Boston, Massachusetts, United States of America
PLoS ONE 6:e14525. 2011..The exact proinflammatory factors, however, that activate the endothelium and their cellular sources remain incompletely defined... - ROCK1 mediates leukocyte recruitment and neointima formation following vascular injuryKensuke Noma
Vascular Medicine Research Unit, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts, USA
J Clin Invest 118:1632-44. 2008..These findings indicate that ROCK1 in BM-derived cells mediates neointima formation following vascular injury and suggest that ROCK1 may represent a promising therapeutic target in vascular inflammatory diseases... - Emerging mechanisms of neutrophil recruitment across endotheliumMarcie R Williams
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, NRB 752P, Boston, MA 02115, USA
Trends Immunol 32:461-9. 2011..This review focuses on these emerging pathways and the mechanisms that regulate neutrophil recruitment across endothelium... - Endothelial CD47 interaction with SIRPgamma is required for human T-cell transendothelial migration under shear flow conditions in vitroMichael Stefanidakis
Department of Pathology, Center for Excellence in Vascular Biology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
Blood 112:1280-9. 2008..Nonetheless, these findings suggest endothelial cell CD47 interacting with T-cell ligands, such as SIRPgamma, play an important role in T-cell transendothelial migration... - Cdc42 interacting protein 4 (CIP4) is essential for integrin-dependent T-cell traffickingSuresh Koduru
Division of Immunology, Children s Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 107:16252-6. 2010.... - Foxp3+-inducible regulatory T cells suppress endothelial activation and leukocyte recruitmentElena Maganto-Garcia
Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
J Immunol 187:3521-9. 2011..These data support the concept that Foxp3(+) iTreg help to regulate inflammation independently of their influence on effector T cells by direct suppression of endothelial activation and leukocyte recruitment... - Human mast cell progenitors use alpha4-integrin, VCAM-1, and PSGL-1 E-selectin for adhesive interactions with human vascular endothelium under flow conditionsJoshua A Boyce
Department of Medicine, Harvard Medical School, Boston, MA, USA
Blood 99:2890-6. 2002..These observations may explain the abundance of MCs at sites of mucosal inflammation, where VCAM-1 and E-selectin are important inducible receptors... - EWI-2 modulates lymphocyte integrin alpha4beta1 functionsTatiana V Kolesnikova
Dana Farber Cancer Institute, Brigham and Women s Hospital, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
Blood 103:3013-9. 2004..We suggest that EWI-2-dependent reorganization of alpha4beta1-CD81 complexes on the cell surface is responsible for EWI-2 effects on integrin-dependent morphology and motility functions... - Mucin AgC10 from Trypanosoma cruzi Interferes with L-selectin-mediated monocyte adhesionPilar Alcaide
Department of Pathology, Center for Excellence in Vascular Biology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
Infect Immun 78:1260-8. 2010..This is the first description of a mechanism that prevents leukocyte adhesion to the endothelium by a parasite and represents a new potential countermeasure to evade the generation of a correct immune response... - Early response of endothelial cells to flow is mediated by VE-cadherinAleksandr Rabodzey
Hatsopoulos Microfluids Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Cell Commun Adhes 14:195-209. 2007.... - Statins suppress apolipoprotein CIII-induced vascular endothelial cell activation and monocyte adhesionChunyu Zheng
Center for Interdisciplinary Cardiovascular Sciences, Brigham and Women s Hospital, Harvard Medical School, 3 Blackfan Circle, CLSB, Floor 17, Boston, MA 02115, USA
Eur Heart J 34:615-24. 2013..Statins may exert lipid-independent anti-inflammatory effects. The present study examined whether statins suppress apoCIII-induced EC activation in vitro and in vivo... - Beta-galactoside alpha2,3-sialyltransferase-I gene expression during Th2 but not Th1 differentiation: implications for core2-glycan formation on cell surface proteinsNir Grabie
Immunology Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue, Room 521, Boston, MA 02115, USA
Eur J Immunol 32:2766-72. 2002..We suggest that lack of ST3Gal-I expression in Th1 cells allows the formation of surface core2 O-glycans and supports their interactions with endothelial selectins... - Regulation of vascular endothelial barrier function by Epac, a cAMP-activated exchange factor for Rap GTPaseXavier Cullere
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
Blood 105:1950-5. 2005..Thus, Epac/Rap activation represents a new pathway for regulating endothelial cell barrier function... - Apolipoprotein CIII in apolipoprotein B lipoproteins enhances the adhesion of human monocytic cells to endothelial cellsAkio Kawakami
Department of Nutrition, Harvard School of Public Health, Boston, MA 02115, USA
Circulation 113:691-700. 2006..Thus, we investigated the direct effect of lipoproteins that do or do not have apoCIII, and apoCIII itself, on adhesion of THP-1 cells, a human monocytic cell line, to vascular endothelial cells (ECs)... 
FAK and PAX-illin get involved in leukocyte diapedesisFrancis W Luscinskas
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
Eur J Immunol 42:296-8. 2012..implicates FAK and paxillin in the proximal (leukocyte rolling) and distal (diapedesis) steps of the multistep adhesion cascade of leukocyte recruitment...- Suppression of experimental autoimmune encephalomyelitis by extracellular adherence protein of Staphylococcus aureusChangping Xie
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
J Exp Med 203:985-94. 2006..Strikingly, intervention with Eap after the onset of EAE suppressed the disease. Collectively, our findings indicate that Eap represents an attractive treatment for autoimmune neuroinflammatory disorders such as MS... - Isolation and culture of murine heart and lung endothelial cells for in vitro model systemsYaw-Chyn Lim
Department of Pathology and Physiology, Immunology Program, National University of Singapore, Singapore
Methods Mol Biol 341:141-54. 2006..Such endothelial cells, therefore, can be used for various in vitro models including leukocyte adhesion assay or in depth biochemical analyses... - CD99 is a key mediator of the transendothelial migration of neutrophilsOlivia Lou
Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
J Immunol 178:1136-43. 2007..Taken together, the evidence demonstrates that PECAM and CD99 regulate distinct, sequential steps in the transendothelial migration of neutrophils during inflammation... - Mechanical forces induced by the transendothelial migration of human neutrophilsAleksandr Rabodzey
Laboratoire Matière et Systèmes Complexes, Universite Paris Diderot, Unité Mixte de Recherche CNRS, Paris, France
Biophys J 95:1428-38. 2008..Our data suggest that the force induced by neutrophil transmigration plays a key role in the disruption of endothelial adherens junctions... - Regulation of leukocyte transmigration: cell surface interactions and signaling eventsYuan Liu
Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA
J Immunol 172:7-13. 2004 - JAM-C regulates unidirectional monocyte transendothelial migration in inflammationPaul F Bradfield
Department of Pathology and Immunology, University Medical Centre, Geneva, Switzerland
Blood 110:2545-55. 2007..All together, our results suggest a novel mechanism of reducing accumulation of monocytes at inflammation sites by disruption of JAM-C-mediated monocyte retention... - Endothelial-dependent mechanisms of leukocyte recruitment to the vascular wallRavi M Rao
Vascular Science, National Heart and Lung Institute, Imperial College School of Medicine, Hammersmith Hospital, London, UK
Circ Res 101:234-47. 2007.... - Crawling and INTEGRating apical cuesRonen Alon
Nat Immunol 5:351-3. 2004