Francis W Luscinskas

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Dendritic cell expression of the transcription factor T-bet regulates mast cell progenitor homing to mucosal tissue
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 204:431-9. 2007
  2. ncbi request reprint C-C and C-X-C chemokines trigger firm adhesion of monocytes to vascular endothelium under flow conditions
    F W Luscinskas
    Department of Pathology, Brigham and Women s Hospital, Boston, Massachusett 02115, USA
    Ann N Y Acad Sci 902:288-93. 2000
  3. ncbi request reprint Leukocyte transendothelial migration: a junctional affair
    Francis W Luscinskas
    Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Semin Immunol 14:105-13. 2002
  4. ncbi request reprint The role of endothelial cell lateral junctions during leukocyte trafficking
    Francis W Luscinskas
    Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Immunol Rev 186:57-67. 2002
  5. pmc p120-Catenin regulates leukocyte transmigration through an effect on VE-cadherin phosphorylation
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
    Blood 112:2770-9. 2008
  6. pmc Endothelial CD47 promotes vascular endothelial-cadherin tyrosine phosphorylation and participates in T cell recruitment at sites of inflammation in vivo
    Veronica Azcutia
    Department of Pathology, Center for Excellence in Vascular Biology, Boston, MA 02115, USA
    J Immunol 189:2553-62. 2012
  7. ncbi request reprint Endothelial cell cortactin coordinates intercellular adhesion molecule-1 clustering and actin cytoskeleton remodeling during polymorphonuclear leukocyte adhesion and transmigration
    Lin Yang
    Center for Excellence in Vascular Biology, Departments of Pathology, Brigham and Women s Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    J Immunol 177:6440-9. 2006
  8. pmc Neutrophil recruitment under shear flow: it's all about endothelial cell rings and gaps
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Microcirculation 16:43-57. 2009
  9. ncbi request reprint Apolipoprotein CIII induces expression of vascular cell adhesion molecule-1 in vascular endothelial cells and increases adhesion of monocytic cells
    Akio Kawakami
    Department of Nutrition, Harvard School of Public Health, 665 Huntington Ave, Boston, MA 02115, USA
    Circulation 114:681-7. 2006
  10. pmc p120-Catenin prevents neutrophil transmigration independently of RhoA inhibition by impairing Src dependent VE-cadherin phosphorylation
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital, Boston, MA, USA
    Am J Physiol Cell Physiol 303:C385-95. 2012

Detail Information

Publications45

  1. pmc Dendritic cell expression of the transcription factor T-bet regulates mast cell progenitor homing to mucosal tissue
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Exp Med 204:431-9. 2007
    ..Therefore, remarkably, T-bet expression by DCs indirectly controls MCp homing to mucosal tissues...
  2. ncbi request reprint C-C and C-X-C chemokines trigger firm adhesion of monocytes to vascular endothelium under flow conditions
    F W Luscinskas
    Department of Pathology, Brigham and Women s Hospital, Boston, Massachusett 02115, USA
    Ann N Y Acad Sci 902:288-93. 2000
    ....
  3. ncbi request reprint Leukocyte transendothelial migration: a junctional affair
    Francis W Luscinskas
    Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Semin Immunol 14:105-13. 2002
    ..This article will focus on the endothelial-dependent processes that coordinate transmigrations in peripheral vasculature during the inflammatory response...
  4. ncbi request reprint The role of endothelial cell lateral junctions during leukocyte trafficking
    Francis W Luscinskas
    Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Immunol Rev 186:57-67. 2002
    ..This article reviews the endothelial-dependent mechanisms that coordinate TEM in peripheral vasculature and highlights the role of certain lateral junctional proteins and protein complexes...
  5. pmc p120-Catenin regulates leukocyte transmigration through an effect on VE-cadherin phosphorylation
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
    Blood 112:2770-9. 2008
    ....
  6. pmc Endothelial CD47 promotes vascular endothelial-cadherin tyrosine phosphorylation and participates in T cell recruitment at sites of inflammation in vivo
    Veronica Azcutia
    Department of Pathology, Center for Excellence in Vascular Biology, Boston, MA 02115, USA
    J Immunol 189:2553-62. 2012
    ..These findings suggest that engagement of endothelial CD47 by its ligands triggers outside-in signals in endothelium that facilitate leukocyte TEM...
  7. ncbi request reprint Endothelial cell cortactin coordinates intercellular adhesion molecule-1 clustering and actin cytoskeleton remodeling during polymorphonuclear leukocyte adhesion and transmigration
    Lin Yang
    Center for Excellence in Vascular Biology, Departments of Pathology, Brigham and Women s Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    J Immunol 177:6440-9. 2006
    ..Our data suggest a model in which tyrosine phosphorylation of cortactin dynamically links ICAM-1 to the actin cytoskeleton, enabling ICAM-1 to form clusters and facilitate leukocyte transmigration...
  8. pmc Neutrophil recruitment under shear flow: it's all about endothelial cell rings and gaps
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Microcirculation 16:43-57. 2009
    ..Interestingly, the signaling events that lead to neutrophil transmigration occur independently of shear flow in vitro...
  9. ncbi request reprint Apolipoprotein CIII induces expression of vascular cell adhesion molecule-1 in vascular endothelial cells and increases adhesion of monocytic cells
    Akio Kawakami
    Department of Nutrition, Harvard School of Public Health, 665 Huntington Ave, Boston, MA 02115, USA
    Circulation 114:681-7. 2006
    ..We recently reported that apoCIII has a proinflammatory effect on human monocytes. In this study, we looked for a direct effect of apoCIII on EC expression of adhesion molecules, leading to monocytic cell adhesion...
  10. pmc p120-Catenin prevents neutrophil transmigration independently of RhoA inhibition by impairing Src dependent VE-cadherin phosphorylation
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital, Boston, MA, USA
    Am J Physiol Cell Physiol 303:C385-95. 2012
    ..In summary, p120 overexpression inhibits neutrophil TEM independently of an effect on RhoA or Rac and instead blocks TEM by preventing VE-cad tyrosine phosphorylation and association of active Src with the VE-cad complex...
  11. ncbi request reprint The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivo
    Pilar Alcaide
    Department of Pathology, Center for Excellence in Vascular Biology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Invest Dermatol 127:1964-72. 2007
    ..These results demonstrate that both PSGL-1 and CD43 are major E-selectin ligands and are likely to be important during leukocyte recruitment in the development of inflammatory reactions...
  12. ncbi request reprint Neutrophil transmigration under shear flow conditions in vitro is junctional adhesion molecule-C independent
    Monica Sircar
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Immunol 178:5879-87. 2007
    ..These data suggest that JAM-C has a minimal role, if any, in PMN transmigration in this model and that ICAM-1 is the preferred endothelial-expressed ligand for PMN beta(2) integrins during transendothelial migration...
  13. pmc Essential role for Rap1 GTPase and its guanine exchange factor CalDAG-GEFI in LFA-1 but not VLA-4 integrin mediated human T-cell adhesion
    Haifa Ghandour
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Blood 110:3682-90. 2007
    ..Thus, PLC/CalDAG-GEFI regulation of Rap1 is selectively required for chemokine- and PMA-induced LFA-1 activation in human T cells, whereas alternate PLC- and PKC-dependent mechanisms are involved in the regulation of VLA-4...
  14. pmc Difference in Th1 and Th17 lymphocyte adhesion to endothelium
    Pilar Alcaide
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Immunol 188:1421-30. 2012
    ..In an air pouch model of inflammation, CCL20 triggered recruitment of Th17 but not Th1 cells. These data provide evidence that E-selectin- and ICAM-1-dependent adhesion of Th17 and Th1 cells with endothelium are quantitatively different...
  15. pmc ICAM-1 regulates neutrophil adhesion and transcellular migration of TNF-alpha-activated vascular endothelium under flow
    Lin Yang
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, NRB 752P, Boston, MA 02215, USA
    Blood 106:584-92. 2005
    ..In summary, ICAM-1 promotes junctional and nonjunctional TEM across inflamed vascular endothelium via distinct cytoplasmic tail associations...
  16. ncbi request reprint Neutrophils from MMP-9- or neutrophil elastase-deficient mice show no defect in transendothelial migration under flow in vitro
    Jennifer R Allport
    Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA
    J Leukoc Biol 71:821-8. 2002
    ..These results indicate that although neutrophil proteases may play a role in the acute inflammatory response, neutrophil elastase or MMP-9 is not essential for neutrophil transendothelial migration in this murine system...
  17. pmc Heterogeneity of endothelial cells from different organ sites in T-cell subset recruitment
    Yaw Chyn Lim
    Department of Pathology, Vascular Research Division, Brigham and Women s Hospital and Harvard Medical School, Boston Massachusetts 02115, USA
    Am J Pathol 162:1591-601. 2003
    ..Furthermore, these results provide an insight into the molecular mechanisms that may mediate T-helper cell recruitment to these organs...
  18. ncbi request reprint Abrogation of functional selectin-ligand expression reduces migration of pathogenic CD8+ T cells into heart
    Yi Hong Cai
    Vascular Research Division, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Immunol 176:6568-75. 2006
    ..These results identify a prominent role for selectin ligands in contributing to effector CD8+ T cell recruitment into the myocardium and indicate that selectin-dependent T cell recruitment is relevant to other tissues besides the skin...
  19. ncbi request reprint Endothelial cell cortactin phosphorylation by Src contributes to polymorphonuclear leukocyte transmigration in vitro
    Lin Yang
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Circ Res 98:394-402. 2006
    ..Moreover, expression of cortactin3F alone partially blocked PMN transmigration. These data suggest a model whereby tyrosine phosphorylation of cortactin by Src family kinases regulates PMN transmigration...
  20. pmc ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury
    Kensuke Noma
    Vascular Medicine Research Unit, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 118:1632-44. 2008
    ..These findings indicate that ROCK1 in BM-derived cells mediates neointima formation following vascular injury and suggest that ROCK1 may represent a promising therapeutic target in vascular inflammatory diseases...
  21. pmc Regulation of endothelial cell adhesion molecule expression by mast cells, macrophages, and neutrophils
    Jie Zhang
    Department of Medicine, Harvard Medical School, Brigham and Women s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 6:e14525. 2011
    ..The exact proinflammatory factors, however, that activate the endothelium and their cellular sources remain incompletely defined...
  22. pmc Elastase release by transmigrating neutrophils deactivates endothelial-bound SDF-1alpha and attenuates subsequent T lymphocyte transendothelial migration
    Ravi M Rao
    Department of Pathology, Center for Excellence in Vascular Biology, Brigham and Women s Hospital, Harvard Medical School, 77 Ave Louis Pasteur NRB 752P, Boston, MA 02115, USA
    J Exp Med 200:713-24. 2004
    ..In summary, transmigrating neutrophils secrete localized elastase that is protected from plasma inhibitors, and thereby modulate trafficking of other leukocyte subsets by altering the endothelial-associated chemotactic activities...
  23. pmc Endothelial TNF receptor 2 induces IRF1 transcription factor-dependent interferon-β autocrine signaling to promote monocyte recruitment
    Deepak Venkatesh
    Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Immunity 38:1025-37. 2013
    ..Thus, our data identify a pathway in endothelial cells that selectively recruits monocytes during a TNF-induced inflammatory response...
  24. pmc Emerging mechanisms of neutrophil recruitment across endothelium
    Marcie R Williams
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, 77 Avenue Louis Pasteur, NRB 752P, Boston, MA 02115, USA
    Trends Immunol 32:461-9. 2011
    ..This review focuses on these emerging pathways and the mechanisms that regulate neutrophil recruitment across endothelium...
  25. pmc Foxp3+-inducible regulatory T cells suppress endothelial activation and leukocyte recruitment
    Elena Maganto-Garcia
    Department of Pathology, Brigham and Women s Hospital, Boston, MA 02115, USA
    J Immunol 187:3521-9. 2011
    ..These data support the concept that Foxp3(+) iTreg help to regulate inflammation independently of their influence on effector T cells by direct suppression of endothelial activation and leukocyte recruitment...
  26. pmc Cdc42 interacting protein 4 (CIP4) is essential for integrin-dependent T-cell trafficking
    Suresh Koduru
    Division of Immunology, Children s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:16252-6. 2010
    ....
  27. pmc Endothelial CD47 interaction with SIRPgamma is required for human T-cell transendothelial migration under shear flow conditions in vitro
    Michael Stefanidakis
    Department of Pathology, Center for Excellence in Vascular Biology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Blood 112:1280-9. 2008
    ..Nonetheless, these findings suggest endothelial cell CD47 interacting with T-cell ligands, such as SIRPgamma, play an important role in T-cell transendothelial migration...
  28. pmc Statins suppress apolipoprotein CIII-induced vascular endothelial cell activation and monocyte adhesion
    Chunyu Zheng
    Center for Interdisciplinary Cardiovascular Sciences, Brigham and Women s Hospital, Harvard Medical School, 3 Blackfan Circle, CLSB, Floor 17, Boston, MA 02115, USA
    Eur Heart J 34:615-24. 2013
    ..Statins may exert lipid-independent anti-inflammatory effects. The present study examined whether statins suppress apoCIII-induced EC activation in vitro and in vivo...
  29. ncbi request reprint Human mast cell progenitors use alpha4-integrin, VCAM-1, and PSGL-1 E-selectin for adhesive interactions with human vascular endothelium under flow conditions
    Joshua A Boyce
    Department of Medicine, Harvard Medical School, Boston, MA, USA
    Blood 99:2890-6. 2002
    ..These observations may explain the abundance of MCs at sites of mucosal inflammation, where VCAM-1 and E-selectin are important inducible receptors...
  30. pmc Mucin AgC10 from Trypanosoma cruzi Interferes with L-selectin-mediated monocyte adhesion
    Pilar Alcaide
    Department of Pathology, Center for Excellence in Vascular Biology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 78:1260-8. 2010
    ..This is the first description of a mechanism that prevents leukocyte adhesion to the endothelium by a parasite and represents a new potential countermeasure to evade the generation of a correct immune response...
  31. ncbi request reprint EWI-2 modulates lymphocyte integrin alpha4beta1 functions
    Tatiana V Kolesnikova
    Dana Farber Cancer Institute, Brigham and Women s Hospital, Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Blood 103:3013-9. 2004
    ..We suggest that EWI-2-dependent reorganization of alpha4beta1-CD81 complexes on the cell surface is responsible for EWI-2 effects on integrin-dependent morphology and motility functions...
  32. ncbi request reprint Early response of endothelial cells to flow is mediated by VE-cadherin
    Aleksandr Rabodzey
    Hatsopoulos Microfluids Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Cell Commun Adhes 14:195-209. 2007
    ....
  33. ncbi request reprint Apolipoprotein CIII in apolipoprotein B lipoproteins enhances the adhesion of human monocytic cells to endothelial cells
    Akio Kawakami
    Department of Nutrition, Harvard School of Public Health, Boston, MA 02115, USA
    Circulation 113:691-700. 2006
    ..Thus, we investigated the direct effect of lipoproteins that do or do not have apoCIII, and apoCIII itself, on adhesion of THP-1 cells, a human monocytic cell line, to vascular endothelial cells (ECs)...
  34. ncbi request reprint Regulation of vascular endothelial barrier function by Epac, a cAMP-activated exchange factor for Rap GTPase
    Xavier Cullere
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
    Blood 105:1950-5. 2005
    ..Thus, Epac/Rap activation represents a new pathway for regulating endothelial cell barrier function...
  35. ncbi request reprint Beta-galactoside alpha2,3-sialyltransferase-I gene expression during Th2 but not Th1 differentiation: implications for core2-glycan formation on cell surface proteins
    Nir Grabie
    Immunology Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue, Room 521, Boston, MA 02115, USA
    Eur J Immunol 32:2766-72. 2002
    ..We suggest that lack of ST3Gal-I expression in Th1 cells allows the formation of surface core2 O-glycans and supports their interactions with endothelial selectins...
  36. pmc Signaling lymphocyte activation molecule regulates development of colitis in mice
    Boaz van Driel
    Division of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA
    Gastroenterology 143:1544-1554.e7. 2012
    ..Slamf1 regulates microbicidal mechanisms in macrophages, therefore we investigated whether the receptor affects development of colitis in mice...
  37. doi request reprint FAK and PAX-illin get involved in leukocyte diapedesis
    Francis W Luscinskas
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Eur J Immunol 42:296-8. 2012
    ..implicates FAK and paxillin in the proximal (leukocyte rolling) and distal (diapedesis) steps of the multistep adhesion cascade of leukocyte recruitment...
  38. pmc Suppression of experimental autoimmune encephalomyelitis by extracellular adherence protein of Staphylococcus aureus
    Changping Xie
    Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    J Exp Med 203:985-94. 2006
    ..Strikingly, intervention with Eap after the onset of EAE suppressed the disease. Collectively, our findings indicate that Eap represents an attractive treatment for autoimmune neuroinflammatory disorders such as MS...
  39. ncbi request reprint CD99 is a key mediator of the transendothelial migration of neutrophils
    Olivia Lou
    Department of Pathology and Laboratory Medicine, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Immunol 178:1136-43. 2007
    ..Taken together, the evidence demonstrates that PECAM and CD99 regulate distinct, sequential steps in the transendothelial migration of neutrophils during inflammation...
  40. pmc JAM-C regulates unidirectional monocyte transendothelial migration in inflammation
    Paul F Bradfield
    Department of Pathology and Immunology, University Medical Centre, Geneva, Switzerland
    Blood 110:2545-55. 2007
    ..All together, our results suggest a novel mechanism of reducing accumulation of monocytes at inflammation sites by disruption of JAM-C-mediated monocyte retention...
  41. ncbi request reprint Endothelial-dependent mechanisms of leukocyte recruitment to the vascular wall
    Ravi M Rao
    Vascular Science, National Heart and Lung Institute, Imperial College School of Medicine, Hammersmith Hospital, London, UK
    Circ Res 101:234-47. 2007
    ....
  42. ncbi request reprint Regulation of leukocyte transmigration: cell surface interactions and signaling events
    Yuan Liu
    Epithelial Pathobiology Research Unit, Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA
    J Immunol 172:7-13. 2004
  43. ncbi request reprint Isolation and culture of murine heart and lung endothelial cells for in vitro model systems
    Yaw Chyn Lim
    Department of Pathology and Physiology, Immunology Program, National University of Singapore, Singapore
    Methods Mol Biol 341:141-54. 2006
    ..Such endothelial cells, therefore, can be used for various in vitro models including leukocyte adhesion assay or in depth biochemical analyses...
  44. pmc Mechanical forces induced by the transendothelial migration of human neutrophils
    Aleksandr Rabodzey
    Laboratoire Matière et Systèmes Complexes, Universite Paris Diderot, Unité Mixte de Recherche CNRS, Paris, France
    Biophys J 95:1428-38. 2008
    ..Our data suggest that the force induced by neutrophil transmigration plays a key role in the disruption of endothelial adherens junctions...
  45. ncbi request reprint Crawling and INTEGRating apical cues
    Ronen Alon
    Nat Immunol 5:351-3. 2004