J Christopher Love

Summary

Affiliation: Harvard Medical School
Country: USA

Publications

  1. doi request reprint Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically
    Kerry Routenberg Love
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, Massachusetts 02139, USA
    Biotechnol Bioeng 106:319-25. 2010
  2. ncbi request reprint A microengraving method for rapid selection of single cells producing antigen-specific antibodies
    J Christopher Love
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Biotechnol 24:703-7. 2006
  3. pmc Tubulation of class II MHC compartments is microtubule dependent and involves multiple endolysosomal membrane proteins in primary dendritic cells
    Jatin M Vyas
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    J Immunol 178:7199-210. 2007
  4. pmc Profiling antibody responses by multiparametric analysis of primary B cells
    Craig M Story
    Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:17902-7. 2008
  5. pmc Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving
    Qing Han
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Bldg 66 456, Cambridge, MA 02139, USA
    Lab Chip 10:1391-400. 2010
  6. doi request reprint Optimization of the surfaces used to capture antibodies from single hybridomas reduces the time required for microengraving
    Jehnna L Ronan
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02139, USA
    J Immunol Methods 340:164-9. 2009
  7. pmc The dynamic lives of T cells: new approaches and themes
    Yvonne J Yamanaka
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Trends Immunol 34:59-66. 2013
  8. pmc Immuno-hybridization chain reaction for enhancing detection of individual cytokine-secreting human peripheral mononuclear cells
    Jonghoon Choi
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Anal Chem 83:6890-5. 2011
  9. pmc Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving
    Vasiliki Panagiotou
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Building 76 253, Cambridge, MA 02139, USA
    Appl Environ Microbiol 77:3154-6. 2011
  10. pmc Cellular barcodes for efficiently profiling single-cell secretory responses by microengraving
    Yvonne J Yamanaka
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Anal Chem 84:10531-6. 2012

Collaborators

  • David A Hafler
  • Bjorn Nilsson
  • Bo Jiang
  • Navin Varadarajan
  • Galit Alter
  • You Me Kim
  • Qing Song
  • Jonghoon Choi
  • Daniel C Douek
  • Gerald T Nepom
  • G M Whitesides
  • Rita Lucia Contento
  • Yvonne J Yamanaka
  • Kerry Routenberg Love
  • Hidde L Ploegh
  • Qing Han
  • Elizabeth M Bradshaw
  • Craig M Story
  • Todd M Gierahn
  • Vasiliki Panagiotou
  • Adebola O Ogunniyi
  • Jehnna L Ronan
  • Eliseo Papa
  • Chih Chi Andrew Hu
  • Jatin M Vyas
  • Katerina Artavanis-Tsakonas
  • Xiaosai Yao
  • Alexis J Torres
  • Douglas A Lauffenburger
  • Ayça Yalçin
  • Yuan Gong
  • Viktor A Adalsteinsson
  • Carla R Lamb
  • William C Hahn
  • Mary Ellen Taplin
  • Atish D Choudhury
  • Matthew S Chabot
  • Tiantian Li
  • K Dane Wittrup
  • Mari Nakabayashi
  • Gwo Shu M Lee
  • Philip W Kantoff
  • Christina A Williamson
  • Jesse S Boehm
  • Sangram Bagh
  • Kai W Wucherpfennig
  • Susana Gordo
  • Patrick C Cheney
  • Kelly F Benedict
  • Christoph T Berger
  • Mavis S N Brefo
  • Talitha L Forcier
  • Darrell J Irvine
  • Neda Bagheri
  • Gregory L Szeto
  • Magdalena Sips
  • Juergen Nett
  • Terrance Stadheim
  • Terrance A Stadheim
  • Annette M McGehee
  • Eduardo Guillen
  • Stephanie K Dougan
  • Kara Herlihy
  • Vinay Tripuraneni
  • Tihamer Orban
  • Sally C Kent
  • Annemarthe G van der Veen

Detail Information

Publications28

  1. doi request reprint Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically
    Kerry Routenberg Love
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, Massachusetts 02139, USA
    Biotechnol Bioeng 106:319-25. 2010
    ..We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner...
  2. ncbi request reprint A microengraving method for rapid selection of single cells producing antigen-specific antibodies
    J Christopher Love
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nat Biotechnol 24:703-7. 2006
    ..This method can be adapted, in principle, to detect any secreted product in a multiplexed manner...
  3. pmc Tubulation of class II MHC compartments is microtubule dependent and involves multiple endolysosomal membrane proteins in primary dendritic cells
    Jatin M Vyas
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    J Immunol 178:7199-210. 2007
    ..In addition to its association with microtubules, endolysosomal tubules follow the plus ends of microtubules as visualized in primary DCs expressing end binding protein 1 (EB1)-enhanced GFP...
  4. pmc Profiling antibody responses by multiparametric analysis of primary B cells
    Craig M Story
    Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:17902-7. 2008
    ....
  5. pmc Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving
    Qing Han
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Bldg 66 456, Cambridge, MA 02139, USA
    Lab Chip 10:1391-400. 2010
    ..These examples demonstrate the utility of quantitative, multidimensional profiles of single cells for analyzing the diversity and dynamics of immune responses in vitro and for identifying rare cells from clinical samples...
  6. doi request reprint Optimization of the surfaces used to capture antibodies from single hybridomas reduces the time required for microengraving
    Jehnna L Ronan
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02139, USA
    J Immunol Methods 340:164-9. 2009
    ..These technical advances will improve the throughput and reduce the costs for this alternative to traditional screening by limiting serial dilution...
  7. pmc The dynamic lives of T cells: new approaches and themes
    Yvonne J Yamanaka
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Trends Immunol 34:59-66. 2013
    ..Such insights into the dynamic properties of T cells should improve immune monitoring and inform strategies for therapeutic interventions...
  8. pmc Immuno-hybridization chain reaction for enhancing detection of individual cytokine-secreting human peripheral mononuclear cells
    Jonghoon Choi
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Anal Chem 83:6890-5. 2011
    ..This approach should enhance the utility of microengraving for defining the immunological signatures of diseases and responses to interventional therapies based on multiplexed single-cell analysis...
  9. pmc Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving
    Vasiliki Panagiotou
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Building 76 253, Cambridge, MA 02139, USA
    Appl Environ Microbiol 77:3154-6. 2011
    ..Clones retrieved following three rounds of mutagenesis yielded titers 2.65-fold greater than those of the parental strain...
  10. pmc Cellular barcodes for efficiently profiling single-cell secretory responses by microengraving
    Yvonne J Yamanaka
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Anal Chem 84:10531-6. 2012
    ....
  11. pmc Functional single-cell analysis of T-cell activation by supported lipid bilayer-tethered ligands on arrays of nanowells
    Alexis J Torres
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Lab Chip 13:90-9. 2013
    ..This new technique should allow detailed investigations on how biophysical and structural aspects of the synapse influence the activation of individual T cells and their complex functional responses...
  12. doi request reprint Analytical technologies for integrated single-cell analysis of human immune responses
    Ayça Yalçin
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    Methods Mol Biol 853:211-35. 2012
    ..The presented approach should provide a basis for the design and implementation of nonconventional bioanalytical processes for studying specific responses of an immune system...
  13. pmc Polyfunctional responses by human T cells result from sequential release of cytokines
    Qing Han
    Department of Chemical Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 109:1607-12. 2012
    ..The trajectories of responses by single cells may also provide unique, time-dependent signatures for immune monitoring that are less compromised by the timing and duration of integrated measures...
  14. pmc Rapid, efficient functional characterization and recovery of HIV-specific human CD8+ T cells using microengraving
    Navin Varadarajan
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 109:3885-90. 2012
    ..This rapid (<24 h), efficient, and inexpensive process should improve the comparative study of human T-cell immunology across ages and anatomic compartments...
  15. doi request reprint Microtools for single-cell analysis in biopharmaceutical development and manufacturing
    Kerry Routenberg Love
    The David H Koch Institute for Integrative Cancer Research, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Trends Biotechnol 31:280-6. 2013
    ..Integrating these advanced technologies into the development of new biologics could greatly reduce time and costs required to bring alternative products to market, and thus expand their global availability...
  16. ncbi request reprint Single-cell analysis of the dynamics and functional outcomes of interactions between human natural killer cells and target cells
    Yvonne J Yamanaka
    Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, MA 02139, USA
    Integr Biol (Camb) 4:1175-84. 2012
    ....
  17. pmc Screening individual hybridomas by microengraving to discover monoclonal antibodies
    Adebola O Ogunniyi
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Nat Protoc 4:767-82. 2009
    ..Screening and retrieval require approximately 1-3 d (9-12 d including the steps for preparing arrays of microwells)...
  18. pmc Massively parallel detection of gene expression in single cells using subnanolitre wells
    Yuan Gong
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Lab Chip 10:2334-7. 2010
    ..The technique should also allow the detection of replicating intracellular pathogens such as retroviruses...
  19. pmc On-chip activation and subsequent detection of individual antigen-specific T cells
    Qing Song
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Anal Chem 82:473-7. 2010
    ..This new method should enable direct enumeration of antigen-specific CD4+ T cells ex vivo from clinical samples...
  20. ncbi request reprint Functional analysis of single cells identifies a rare subset of circulating tumor cells with malignant traits
    Xiaosai Yao
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Integr Biol (Camb) 6:388-98. 2014
    ..Every CTC observed, however, did not exhibit such metastatic potential, suggesting that enumeration of CTCs alone may be insufficient to understand metastasis or stratify patients. ..
  21. pmc A high-throughput single-cell analysis of human CD8⁺ T cell functions reveals discordance for cytokine secretion and cytolysis
    Navin Varadarajan
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    J Clin Invest 121:4322-31. 2011
    ..Our approach should facilitate determination of signatures of functional variance among individual effector CD8+ T cells, including those from mucosal samples and those induced by vaccines...
  22. pmc XBP-1 regulates signal transduction, transcription factors and bone marrow colonization in B cells
    Chih Chi Andrew Hu
    Department of Biology, Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    EMBO J 28:1624-36. 2009
    ..They fail to colonize the bone marrow and do not sustain antibody production. These findings define the role of XBP-1 in normal plasma cell development and have implications for management of B-cell malignancies...
  23. pmc Recruitment of CD63 to Cryptococcus neoformans phagosomes requires acidification
    Katerina Artavanis-Tsakonas
    Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 103:15945-50. 2006
    ..These observations indicate that the composition of distinct phagosomal compartments within the same cell is determined by phagosomal cargo and may affect the outcome of antigen processing and presentation...
  24. ncbi request reprint Molecular engineering of surfaces using self-assembled monolayers
    George M Whitesides
    Harvard University, Department of Chemistry and Chemical Biology, Cambridge, MA 02138 2902, USA
    Sci Prog 88:17-48. 2005
    ..Understanding the principles by which they form, and connecting molecular-level structure with macroscopic properties, opens a wide range of areas to study and exploitation...
  25. ncbi request reprint Self-assembled monolayers of thiolates on metals as a form of nanotechnology
    J Christopher Love
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    Chem Rev 105:1103-69. 2005
  26. ncbi request reprint Self-assembled monolayers of alkanethiolates on palladium are good etch resists
    J Christopher Love
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    J Am Chem Soc 124:1576-7. 2002
    ..Characterization of the SAM formed from hexadecanethiol on palladium is described...
  27. pmc Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies
    Elizabeth M Bradshaw
    Division of Molecular Immunology, Center for Neurologic Diseases, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Clin Immunol 129:10-8. 2008
    ..These data demonstrate the utility of microengraving for interrogating multiple phenotypes of single human cells concurrently and for detecting rare populations of cells by their secreted products...
  28. ncbi request reprint Formation and structure of self-assembled monolayers of alkanethiolates on palladium
    J Christopher Love
    Department of Chemistry and the Fredrick Seitz Materials Research Laboratory at the University of Illinois Urbana Champaign, Urbana, IL 61801, USA
    J Am Chem Soc 125:2597-609. 2003
    ..The differences in chain organization between SAMs formed by microcontact printing and by solution deposition are also examined by RAIRS and XPS...