J Lieberman

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Engineered Listeria monocytogenes as an AIDS vaccine
    Judy Lieberman
    The Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
    Vaccine 20:2007-10. 2002
  2. ncbi request reprint The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal
    Judy Lieberman
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Immunol 3:361-70. 2003
  3. ncbi request reprint Expansion of CD57 and CD62L-CD45RA+ CD8 T lymphocytes correlates with reduced viral plasma RNA after primary HIV infection
    J Lieberman
    The Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    AIDS 13:891-9. 1999
  4. ncbi request reprint Interfering with disease: opportunities and roadblocks to harnessing RNA interference
    Judy Lieberman
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Trends Mol Med 9:397-403. 2003
  5. ncbi request reprint Tracking the killers: how should we measure CD8 T cells in HIV infection?
    Judy Lieberman
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    AIDS 18:1489-93. 2004
  6. ncbi request reprint Avoiding the kiss of death: how HIV and other chronic viruses survive
    Judy Lieberman
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
    Curr Opin Immunol 14:478-86. 2002
  7. ncbi request reprint Dressed to kill? A review of why antiviral CD8 T lymphocytes fail to prevent progressive immunodeficiency in HIV-1 infection
    J Lieberman
    Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    Blood 98:1667-77. 2001
  8. ncbi request reprint Induction of rapid histone degradation by the cytotoxic T lymphocyte protease Granzyme A
    D Zhang
    Center for Blood Research, Massachusetts General Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:3683-90. 2001
  9. ncbi request reprint Impaired function of circulating HIV-specific CD8(+) T cells in chronic human immunodeficiency virus infection
    P Shankar
    Center for Blood Research, Harvard Medical School, and the New England Medical Center, Tufts University School of Medicine, Boston, MA, USA
    Blood 96:3094-101. 2000
  10. pmc Effector differentiation is not prerequisite for generation of memory cytotoxic T lymphocytes
    N Manjunath
    The Center for Blood Research, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 108:871-8. 2001

Research Grants

  1. Apoptosis and Immunity
    Judy Lieberman; Fiscal Year: 2002
  2. Developing RNA Interference for and HIV microbicide
    Judy Lieberman; Fiscal Year: 2005
  3. RNA INTERFERENCE AS A WEAPON AGAINST BIOTERRORISM
    Judy Lieberman; Fiscal Year: 2007
  4. Engineered Listeria monocytogenes as an AIDS vaccine
    Judy Lieberman; Fiscal Year: 2007
  5. Biocontained ImageStream 100 Multispectral Imaging Cytometer
    Judy Lieberman; Fiscal Year: 2007

Detail Information

Publications89

  1. ncbi request reprint Engineered Listeria monocytogenes as an AIDS vaccine
    Judy Lieberman
    The Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
    Vaccine 20:2007-10. 2002
    ..Lmdd-gag immunization protects mice from vaccinia-gag challenge and induces mucosal CTL, even after systemic immunization...
  2. ncbi request reprint The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal
    Judy Lieberman
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Rev Immunol 3:361-70. 2003
    ..In this review, I discuss recent insights into the mechanisms of granule-mediated cytotoxicity, focusing on how granzymes A, B and C and granulysin activate cell death through caspase-independent pathways...
  3. ncbi request reprint Expansion of CD57 and CD62L-CD45RA+ CD8 T lymphocytes correlates with reduced viral plasma RNA after primary HIV infection
    J Lieberman
    The Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    AIDS 13:891-9. 1999
    ..We examined whether other immunological parameters might influence the viral setpoint...
  4. ncbi request reprint Interfering with disease: opportunities and roadblocks to harnessing RNA interference
    Judy Lieberman
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Trends Mol Med 9:397-403. 2003
    ..RNAi could provide an exciting new therapeutic modality for treating infection, cancer, neurodegenerative disease and other illnesses...
  5. ncbi request reprint Tracking the killers: how should we measure CD8 T cells in HIV infection?
    Judy Lieberman
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    AIDS 18:1489-93. 2004
  6. ncbi request reprint Avoiding the kiss of death: how HIV and other chronic viruses survive
    Judy Lieberman
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
    Curr Opin Immunol 14:478-86. 2002
    ..The chance to eradicate an infection by T cell mediated lysis is undermined after an infection becomes established, in part because the effector immune response is impaired in the setting of chronic antigen...
  7. ncbi request reprint Dressed to kill? A review of why antiviral CD8 T lymphocytes fail to prevent progressive immunodeficiency in HIV-1 infection
    J Lieberman
    Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    Blood 98:1667-77. 2001
    ..Functional defects are qualitatively different and more severe with advanced disease, when interferon gamma production also becomes compromised...
  8. ncbi request reprint Induction of rapid histone degradation by the cytotoxic T lymphocyte protease Granzyme A
    D Zhang
    Center for Blood Research, Massachusetts General Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:3683-90. 2001
    ..Histone digestion provides a mechanism for unfolding compacted chromatin and facilitating endogenous DNase access to DNA during T cell and natural killer cell granule-mediated apoptosis...
  9. ncbi request reprint Impaired function of circulating HIV-specific CD8(+) T cells in chronic human immunodeficiency virus infection
    P Shankar
    Center for Blood Research, Harvard Medical School, and the New England Medical Center, Tufts University School of Medicine, Boston, MA, USA
    Blood 96:3094-101. 2000
    ..These results suggest that a significant proportion of HIV-specific CD8 T cells may be functionally compromised in vivo and that some function can be restored by exposure to IL-2...
  10. pmc Effector differentiation is not prerequisite for generation of memory cytotoxic T lymphocytes
    N Manjunath
    The Center for Blood Research, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    J Clin Invest 108:871-8. 2001
    ..In addition to providing a simple means to generate memory cells in virtually unlimited numbers, our results suggest that effector differentiation is not a prerequisite for memory cell generation...
  11. ncbi request reprint gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair
    Dipanjan Chowdhury
    CBR Institute for Biomedical Research and The Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 20:801-9. 2005
    ..The effect of PP2A on gamma-H2AX levels is independent of ATM, ATR, or DNA-PK activity...
  12. ncbi request reprint Granzyme A activates an endoplasmic reticulum-associated caspase-independent nuclease to induce single-stranded DNA nicks
    P J Beresford
    Center for Blood Research and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 276:43285-93. 2001
    ..Thus, GzmA activates a DNase (GzmA-activated DNase) within the SET complex to produce a novel form of DNA damage during cytotoxic T lymphocyte-mediated death...
  13. pmc miR-24-mediated downregulation of H2AX suppresses DNA repair in terminally differentiated blood cells
    Ashish Lal
    Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    Nat Struct Mol Biol 16:492-8. 2009
    ..Therefore, miR-24 upregulation in postreplicative cells reduces H2AX and makes them vulnerable to DNA damage...
  14. ncbi request reprint Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor
    Zusen Fan
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell 112:659-72. 2003
    ..GzmA-treated cells with silenced NM23-H1 expression are resistant to GzmA-mediated DNA damage and cytolysis, while cells overexpressing NM23-H1 are more sensitive...
  15. ncbi request reprint A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 439:497-501. 2006
    ..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint...
  16. ncbi request reprint CD8 T cells specific for human immunodeficiency virus, Epstein-Barr virus, and cytomegalovirus lack molecules for homing to lymphoid sites of infection
    G Chen
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA, USA
    Blood 98:156-64. 2001
    ..Lack of CD45RA expression may indicate incomplete differentiation of HIV-specific CD8 T cells to cytotoxic T cells...
  17. pmc Genetically modified anthrax lethal toxin safely delivers whole HIV protein antigens into the cytosol to induce T cell immunity
    Y Lu
    Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 97:8027-32. 2000
    ..These results lay the foundation for future clinical vaccine studies...
  18. ncbi request reprint Knocking down disease with siRNAs
    Derek M Dykxhoorn
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell 126:231-5. 2006
    ..This Essay will examine the opportunities for harnessing RNA interference (RNAi) for therapy, as well as the obstacles and possible ways to circumvent them...
  19. pmc Live attenuated Listeria monocytogenes expressing HIV Gag: immunogenicity in rhesus monkeys
    Shisong Jiang
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Vaccine 25:7470-9. 2007
    ....
  20. pmc HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme A
    Zusen Fan
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 22:2810-20. 2002
    ....
  21. ncbi request reprint Cleaving the oxidative repair protein Ape1 enhances cell death mediated by granzyme A
    Zusen Fan
    Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    Nat Immunol 4:145-53. 2003
    ..In so doing, GzmA may block cellular repair and force apoptosis. In support of this, cells with silenced Ape1 expression are more sensitive, whereas cells overexpressing noncleavable Ape1 are more resistant, to GzmA-mediated death...
  22. pmc Lentiviral delivery of short hairpin RNAs protects CD4 T cells from multiple clades and primary isolates of HIV
    Sang Kyung Lee
    The CBR Institute for Biomedical Research, 800 Huntington Ave, Boston, MA 02115, USA
    Blood 106:818-26. 2005
    ..Thus, although 1 or 2 mutations at peripheral sites are tolerated, mutations in the central target cleavage region abolish RNAi activity...
  23. ncbi request reprint The gp49B1 inhibitory receptor regulates the IFN-gamma responses of T cells and NK cells
    Xiaogang Gu
    Center for Blood Research and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 170:4095-101. 2003
    ..We conclude that gp49B1 is a unique inhibitory receptor that is induced in multiple lineages of innate and adaptive immune cells during an infection and controls their IFN-gamma, but not cytotoxic responses...
  24. ncbi request reprint Most antiviral CD8 T cells during chronic viral infection do not express high levels of perforin and are not directly cytotoxic
    Dong Zhang
    Center for Blood Research, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Blood 101:226-35. 2003
    ..Impaired antiviral cytotoxicity during chronic infection is not specific to HIV but likely represents the immune response to chronic antigenic exposure...
  25. pmc Granzyme A, which causes single-stranded DNA damage, targets the double-strand break repair protein Ku70
    Pengcheng Zhu
    The CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    EMBO Rep 7:431-7. 2006
    ..Therefore, Ku70 has other antiapoptotic functions in GzmA-induced cell death, which are blocked when GzmA proteolyses Ku70...
  26. ncbi request reprint Cross-clade CD8 T-cell responses to HIV(IIIB) and Chinese B' and C/B' viruses in North American and Chinese HIV-seropositive donors
    Vanessa Fran├žois-Bongar├žon
    CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
    J Acquir Immune Defic Syndr 37:1435-44. 2004
    ..T cells, selected for IFN-gamma production to IIIB virus, also efficiently lysed cells infected with Chinese viruses. Therefore, cross-clade CD8 T-cell responses to IIIB virus and prevalent Chinese viral strains are common...
  27. ncbi request reprint Focal adhesion kinase is required for CXCL12-induced chemotactic and pro-adhesive responses in hematopoietic precursor cells
    A M Glodek
    Department of Pathology, Joint Program in Transfusion Medicine, Children s Hospital Boston, Boston, MA, USA
    Leukemia 21:1723-32. 2007
    ..These studies suggest that FAK may function as a key intermediary in signaling pathways controlling hematopoietic cell lodgment and lineage development...
  28. pmc Granzymes A and B directly cleave lamins and disrupt the nuclear lamina during granule-mediated cytolysis
    D Zhang
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 98:5746-51. 2001
    ..Lamins A/C are direct substrates of GzmA, but not GzmB. GzmA and GzmB therefore directly target critical caspase substrates in caspase-resistant cells...
  29. ncbi request reprint The silent treatment: siRNAs as small molecule drugs
    D M Dykxhoorn
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Gene Ther 13:541-52. 2006
    ..This review will discuss progress in developing and testing small RNAi-based drugs and potential obstacles...
  30. pmc The Bcl-2 family pro-apoptotic molecule, BNIP3 regulates activation-induced cell death of effector cytotoxic T lymphocytes
    J Wan
    The Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
    Immunology 110:10-7. 2003
    ..Finally, retroviral transduction of BNIP3 antisense RNA diminished AICD in effector CTL. These results suggest that BNIP3 may play an important role in T-cell homeostasis by regulating effector CTL numbers...
  31. pmc Granzyme A activates another way to die
    Judy Lieberman
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Harvard Medical School, Boston, MA, USA
    Immunol Rev 235:93-104. 2010
    ..GzmA also has proinflammatory activity; it activates pro-interleukin-1beta and may also have other proinflammatory effects that remain to be elucidated...
  32. pmc Prior exposure to an attenuated Listeria vaccine does not reduce immunogenicity: pre-clinical assessment of the efficacy of a Listeria vaccine in the induction of immune responses against HIV
    James B Whitney
    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA
    J Immune Based Ther Vaccines 9:2. 2011
    ..abstract:..
  33. ncbi request reprint The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death
    Dipanjan Chowdhury
    CBR Institute for Biomedical Research, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 23:133-42. 2006
    ..After granzyme A activates NM23-H1 to make single-stranded nicks, TREX1 removes nucleotides from the nicked 3' end to reduce the possibility of repair by rejoining the nicked ends...
  34. ncbi request reprint Running interference: prospects and obstacles to using small interfering RNAs as small molecule drugs
    Derek M Dykxhoorn
    CBR Institute for Biomedical Research, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Biomed Eng 8:377-402. 2006
    ..Pilot siRNA clinical studies began just three years after the discovery that RNAi works in mammalian cells. This review discusses recent progress and obstacles to using siRNAs as small molecule drugs...
  35. pmc miR-24 Inhibits cell proliferation by targeting E2F2, MYC, and other cell-cycle genes via binding to "seedless" 3'UTR microRNA recognition elements
    Ashish Lal
    Immune Disease Institute, Children s Hospital Boston, Department of Pediatrics, Harvard Medical School, MA 02115, USA
    Mol Cell 35:610-25. 2009
    ..The E2F2 3'UTR lacks a predicted miR-24 recognition element. In fact, miR-24 regulates expression of E2F2, MYC, AURKB, CCNA2, CDC2, CDK4, and FEN1 by recognizing seedless but highly complementary sequences...
  36. pmc NFAT5 regulates HIV-1 in primary monocytes via a highly conserved long terminal repeat site
    Shahin Ranjbar
    CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 2:e130. 2006
    ..Inhibition of the NFAT5-LTR interaction may thus present a novel therapeutic target to suppress HIV-1 replication and progression of AIDS...
  37. pmc A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication
    Dipanjan Chowdhury
    Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 31:33-46. 2008
    ..Therefore, gamma-H2AX elimination at DNA damage foci is required for DNA damage repair, but accomplishing this task involves distinct phosphatases with potentially overlapping roles...
  38. pmc Death by a thousand cuts: granzyme pathways of programmed cell death
    Dipanjan Chowdhury
    Dana Farber Cancer Institute and Department of Radiation Oncology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Immunol 26:389-420. 2008
    ..This review discusses what is known about granzyme-mediated pathways of cell death as well as recent studies that implicate granzymes in immune regulation and extracellular proteolytic functions in inflammation...
  39. doi request reprint Identification of host proteins required for HIV infection through a functional genomic screen
    Abraham L Brass
    Department of Genetics, Center for Genetics and Genomics, Brigham and Women s Hospital, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 319:921-6. 2008
    ..This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing identify potential targets for therapy...
  40. ncbi request reprint Nuclear war: the granzyme A-bomb
    Judy Lieberman
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
    Curr Opin Immunol 15:553-9. 2003
    ..The novel cell death pathway initiated by granzyme A provides a parallel pathway for apoptosis, important in destroying targets that overexpress bcl-2 or are otherwise invulnerable to the caspases...
  41. ncbi request reprint The silent revolution: RNA interference as basic biology, research tool, and therapeutic
    Derek M Dykxhoorn
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Med 56:401-23. 2005
    ..RNAi may provide an important new therapeutic modality for treating infection, cancer, neurodegenerative disease, and other illnesses, although in vivo delivery of small interfering RNAs into cells remains a significant obstacle...
  42. pmc Increased expression of the natural killer cell inhibitory receptor CD85j/ILT2 on antigen-specific effector CD8 T cells and its impact on CD8 T-cell function
    M Nedim Ince
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Immunology 112:531-42. 2004
    ..Thus, ILT2 expression, increased on antiviral CD8 cells in chronic infection, may interfere with protective CD8 T-cell function by suppressing IFN-gamma production...
  43. pmc Silencing viral infection
    Derek M Dykxhoorn
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Med 3:e242. 2006
  44. pmc The cytotoxic T lymphocyte protease granzyme A cleaves and inactivates poly(adenosine 5'-diphosphate-ribose) polymerase-1
    Pengcheng Zhu
    Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Blood 114:1205-16. 2009
    ..Disrupting PARP-1, which is also a caspase target, is therefore required for efficient apoptosis by both caspase-independent and caspase-dependent pathways...
  45. pmc Selective gene silencing in activated leukocytes by targeting siRNAs to the integrin lymphocyte function-associated antigen-1
    Dan Peer
    CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:4095-100. 2007
    ....
  46. ncbi request reprint Micromanipulating cancer: microRNA-based therapeutics?
    Fabio Petrocca
    Immune Disease Institute, Program in Cellular and Molecular Medicine, Children s Hospital Boston and Department of Pediatrics, Harvard Medical School, Boston, MA, USA
    RNA Biol 6:335-40. 2009
    ..This review will explore some of the opportunities and obstacles to harnessing microRNA biology for cancer therapy...
  47. doi request reprint miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53
    Francisco Navarro
    Immune Disease Institute and Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Blood 114:2181-92. 2009
    ..However, in p53-null K562 cells, phorbol esters induce miR-34a expression independently of p53 by activating an alternative phorbol ester-responsive promoter to produce a longer pri-miR-34a transcript...
  48. ncbi request reprint Delivering the kiss of death: progress on understanding how perforin works
    Matthew E Pipkin
    CBR Institute for Biomedical Research, Harvard Medical School, Boston MA 02115, USA
    Curr Opin Immunol 19:301-8. 2007
    ..Recently, an alternative model has been proposed that involves active target cell collaboration with perforin to deliver granzymes and direct the target cell to an apoptotic, rather than necrotic, death...
  49. pmc Sustained small interfering RNA-mediated human immunodeficiency virus type 1 inhibition in primary macrophages
    Erwei Song
    Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 77:7174-81. 2003
    ..Under these circumstances, long-term suppression of HIV replication could be achieved with p24 siRNA. Thus, siRNAs can induce potent and long-lasting HIV inhibition in nondividing cells such as macrophages...
  50. doi request reprint Desperately seeking microRNA targets
    Marshall Thomas
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA
    Nat Struct Mol Biol 17:1169-74. 2010
    ..Bioinformatic analysis of over-represented pathways and nodes in protein-DNA interactomes formed from experimental candidate miRNA gene target lists can focus attention on biologically significant target genes...
  51. ncbi request reprint RNA interference targeting Fas protects mice from fulminant hepatitis
    Erwei Song
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    Nat Med 9:347-51. 2003
    ..Silencing Fas expression with RNAi holds therapeutic promise to prevent liver injury by protecting hepatocytes from cytotoxicity...
  52. ncbi request reprint Granzyme B binds to target cells mostly by charge and must be added at the same time as perforin to trigger apoptosis
    Lianfa Shi
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 174:5456-61. 2005
    ..Therefore, PFN must be coendocytosed with GzmB to deliver it into the cytosol...
  53. ncbi request reprint The prospect of silencing disease using RNA interference
    Premlata Shankar
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Mass 02115, USA
    JAMA 293:1367-73. 2005
    ..In this article we discuss the basic mechanism of RNAi and the therapeutic opportunities and obstacles for harnessing RNAi for therapy of human disease...
  54. ncbi request reprint Three regions of HIV-1 gp160 contain clusters of immunodominant CTL epitopes
    P Shankar
    Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    Immunol Lett 52:23-30. 1996
    ..CTL from four subjects recognized at least three distinct epitopes in a.a 591-600 in the context of A24, B8, B14, and B27. CTL from seven subjects recognized epitopes within a.a. 844-863 restricted by A30, B7, B8 and B35...
  55. pmc Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of them
    P J Beresford
    Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 94:9285-90. 1997
    ..PHAP II begins to be degraded within minutes of initiation of cytotoxic T lymphocyte attack. PHAP I and II are candidate participants in the granzyme A pathway of cell-mediated cytotoxicity...
  56. doi request reprint Micromanagers of immune cell fate and function
    Fabio Petrocca
    Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    Adv Immunol 102:227-44. 2009
    ..MicroRNAs (miRNAs) regulate cell fate during development and in response to environmental cues. Here, we review the emerging story of how miRNAs regulate immune cell development and function...
  57. pmc The SET complex acts as a barrier to autointegration of HIV-1
    Nan Yan
    Department of Pediatrics, Immune Disease Institute, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 5:e1000327. 2009
    ..Therefore, the SET complex facilitates HIV-1 infection by preventing suicidal autointegration...
  58. pmc Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity
    Ming Chieh Shun
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    J Virol 82:11555-67. 2008
    ..This initial systematic mutagenesis of a PWWP domain identifies amino acid residues critical for chromatin binding function and the consequences of their changes on HIV-1 integration and infection...
  59. ncbi request reprint Perforin triggers a plasma membrane-repair response that facilitates CTL induction of apoptosis
    Dennis Keefe
    The CBR Institute for Biomedical Research, Boston, MA 02115, USA
    Immunity 23:249-62. 2005
    ..Thus, the target cell actively participates in determining its own fate during cell-mediated death...
  60. ncbi request reprint Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors
    Erwei Song
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Nat Biotechnol 23:709-17. 2005
    ..Furthermore, an ErbB2 single-chain antibody fused with protamine delivered siRNAs specifically into ErbB2-expressing cancer cells. This study demonstrates the potential for systemic, cell-type specific, antibody-mediated siRNA delivery...
  61. pmc Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry
    Fariyal Ahmed
    Immune Disease Institute, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    J Immunol Methods 347:79-86. 2009
    ..However, CD3epsilon exhibited longer residence times (>8 min) at the synapse than Lck...
  62. doi request reprint Small RNAs guide hematopoietic cell differentiation and function
    Francisco Navarro
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 184:5939-47. 2010
    ..This review summarizes our current knowledge of how miRNAs function in normal and malignant hematopoiesis and how miRNAs might be applied for disease treatment...
  63. pmc Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death
    Denis Martinvalet
    Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell 133:681-92. 2008
    ..Target cells expressing a cleavage site mutant of NDUFS3 are resistant to GzmA-mediated cell death but remain sensitive to GzmB...
  64. ncbi request reprint Defying death--HIV mutation to evade cytotoxic T lymphocytes
    Judy Lieberman
    Center for Blood Research, Boston, MA 02115, USA
    N Engl J Med 347:1203-4. 2002
  65. pmc The cytosolic exonuclease TREX1 inhibits the innate immune response to human immunodeficiency virus type 1
    Nan Yan
    Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
    Nat Immunol 11:1005-13. 2010
    ..HIV-stimulated interferon production in cells deficient in TREX1 did not involve known nucleic acid sensors...
  66. doi request reprint Isolation of cytotoxic T cell and NK granules and purification of their effector proteins
    Jerome Thiery
    Program in Cellular and Molecular Medicine, Children s Hospital and Immune Disease Institute, Boston, Massachusetts, USA
    Curr Protoc Cell Biol . 2010
    ..Protocols are also given for purifying the key cytolytic molecules (perforin, granzyme A, granzyme B, and granulysin) from isolated cytotoxic granules by fast protein liquid chromatography...
  67. pmc Durable protection from Herpes Simplex Virus-2 transmission following intravaginal application of siRNAs targeting both a viral and host gene
    Yichao Wu
    The Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell Host Microbe 5:84-94. 2009
    ..Combining siRNAs targeting a viral and host gene protects mice from HSV-2 for a week, irrespective of the time of challenge. Therefore, intravaginal siRNAs could provide sustained protection against viral transmission...
  68. pmc Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expression
    Mun Chun Chan
    Molecular Cardiology Research Institute, Tufts Medical Center, Boston, MA 02111, USA
    EMBO J 29:559-73. 2010
    ..Thus, this study provides a molecular basis for the antagonism between the PDGF and TGFbeta pathways, and its effect on the control of the vSMC phenotype...
  69. pmc The functional CD8 T cell response to HIV becomes type-specific in progressive disease
    Sang Kyung Lee
    Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Invest 110:1339-47. 2002
    ..Therefore, in advanced-stage patients, viral-specific CD8 T cells recognizing consensus epitopes persist from an earlier response but no longer effectively recognize autologous virus...
  70. doi request reprint RNA interference and cancer: endogenous pathways and therapeutic approaches
    Derek M Dykxhoorn
    Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Adv Exp Med Biol 615:299-329. 2008
    ..Possible strategies and obstacles to harnessing RNAi for cancer therapy will also be discussed...
  71. ncbi request reprint An siRNA-based microbicide protects mice from lethal herpes simplex virus 2 infection
    Deborah Palliser
    CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 439:89-94. 2006
    ..These results suggest that siRNAs are attractive candidates for the active component of a microbicide designed to prevent viral infection or transmission...
  72. pmc Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphism
    Derek M Dykxhoorn
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:5953-8. 2006
    ....
  73. ncbi request reprint Granzyme A induces caspase-independent mitochondrial damage, a required first step for apoptosis
    Denis Martinvalet
    The CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Immunity 22:355-70. 2005
    ..Superoxide scavengers also block apoptosis by CTLs expressing GzmA and/or GzmB. Therefore, mitochondrial damage is an essential first step in killer cell granule-mediated pathways of apoptosis...
  74. pmc Vaccinia virus induces strong immunoregulatory cytokine production in healthy human epidermal keratinocytes: a novel strategy for immune evasion
    Luzheng Liu
    Harvard Skin Disease Research Center, Department of Dermatology, Brigham and Women s Hospital, 77 Avenue Louis Pasteur, Suite 672, Boston, MA 02115, USA
    J Virol 79:7363-70. 2005
    ....
  75. ncbi request reprint HIV-1 antiviral activity of recombinant natural killer cell enhancing factors, NKEF-A and NKEF-B, members of the peroxiredoxin family
    Ralf Geiben-Lynn
    Partners AIDS Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02129, USA
    J Biol Chem 278:1569-74. 2003
    ....
  76. pmc miR-200 enhances mouse breast cancer cell colonization to form distant metastases
    Derek M Dykxhoorn
    Department of Pediatrics, Immune Disease Institute, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 4:e7181. 2009
    ..Because some miRNAs are dysregulated in cancer and affect cellular transformation, tumor formation, and metastasis, we examined whether changes in miRNA expression might explain the differences in metastasis of these cells...
  77. pmc Whither or wither microbicides?
    Robert M Grant
    J David Gladstone Institutes, University of California San Francisco, San Francisco, CA 94518, USA
    Science 321:532-4. 2008
    ....
  78. ncbi request reprint Public health. A sound rationale needed for phase III HIV-1 vaccine trials
    Dennis R Burton
    Scripps Research Institute, La Jolla, California, USA
    Science 303:316. 2004
  79. ncbi request reprint siRNA-directed inhibition of HIV-1 infection
    Carl D Novina
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Med 8:681-6. 2002
    ..siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections...
  80. ncbi request reprint Interfering with disease: a progress report on siRNA-based therapeutics
    Antonin de Fougerolles
    Alnylam Pharmaceuticals Inc, 300 Third Street, Cambridge, Massachusetts 02142, USA
    Nat Rev Drug Discov 6:443-53. 2007
    ..We conclude by reviewing the latest clinical experience with RNAi therapeutics...
  81. ncbi request reprint Proliferation responses to HIVp24 during antiretroviral therapy do not reflect improved immune phenotype or function
    Christoph G Lange
    Center for AIDS Research, Department of Medicine, Division of Infectious Diseases, Case Western Reserve University, University Hospitals of Cleveland, 2061 Cornell Road, Cleveland, OH 44106, USA
    AIDS 18:605-13. 2004
    ....
  82. pmc Adenovirus protein VII functions throughout early phase and interacts with cellular proteins SET and pp32
    Yuming Xue
    Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA
    J Virol 79:2474-83. 2005
    ..Interestingly, disappearance of the dots required ongoing RNA synthesis but not DNA synthesis. Taken together these data indicate that protein VII has an ongoing role during early phase and the beginning of DNA replication...
  83. ncbi request reprint let-7 regulates self renewal and tumorigenicity of breast cancer cells
    Fengyan Yu
    Department of Breast Surgery, No 2 Affiliated Hospital, Sun Yat Sen University, Guangzhou 510120, People s Republic of China
    Cell 131:1109-23. 2007
    ..Therefore let-7 regulates multiple BT-IC stem cell-like properties by silencing more than one target...
  84. pmc Small interfering RNA targeting Fas protects mice against renal ischemia-reperfusion injury
    Peter Hamar
    Institute of Pathophysiology, Semmelweis University, Nagyvarad ter 4, Budapest, H 1089, Hungary
    Proc Natl Acad Sci U S A 101:14883-8. 2004
    ..This study confirms the importance of Fas-mediated apoptosis in renal ischemia-reperfusion injury. Silencing Fas by systemic or local catheterization holds therapeutic promise to limit ischemia-reperfusion injury...
  85. ncbi request reprint Your health in the 21st century. Silencing bad genes
    Anthony Komaroff
    Newsweek 145:51-2. 2005
  86. ncbi request reprint Listeria-infected myeloid dendritic cells produce IFN-beta, priming T cell activation
    Hanping Feng
    CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 175:421-32. 2005
    ..Exposure to high concentrations of IFN-beta sensitizes naive T cells for Ag-dependent activation...
  87. ncbi request reprint A mutation in TREX1 that impairs susceptibility to granzyme A-mediated cell death underlies familial chilblain lupus
    Min Ae Lee-Kirsch
    Klinik fur Kinder und Jugendmedizin, Technische Universitat Dresden, Fetscherstr 74, 01307, Dresden, Germany
    J Mol Med (Berl) 85:531-7. 2007
    ..Our findings also warrant further investigation of TREX1 in common forms of lupus erythematosus...
  88. ncbi request reprint Mapping cross-clade HIV-1 vaccine epitopes using a bioinformatics approach
    Anne S De Groot
    TB HIV Research Lab, Brown University, Providence, RI 02912, USA
    Vaccine 21:4486-504. 2003
    ..Putative HIV-1 CTL epitopes were selected from this list using the epitope prediction tool EpiMatrix...
  89. pmc A fragment of anthrax lethal factor delivers proteins to the cytosol without requiring protective antigen
    Nicholas Kushner
    Harvard AIDS Institute and Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6652-7. 2003
    ..Furthermore, these results enable us to propose a modified molecular mechanism of anthrax lethal toxin...

Research Grants7

  1. Apoptosis and Immunity
    Judy Lieberman; Fiscal Year: 2002
    ..abstract_text> ..
  2. Developing RNA Interference for and HIV microbicide
    Judy Lieberman; Fiscal Year: 2005
    ..test siRNA delivery strategies in the human skin blister model; 3. test siRNAs and siRNA delivery strategies for silencing SIV gag and CCR5 in PBMCs from pig-tailed macaques. ..
  3. RNA INTERFERENCE AS A WEAPON AGAINST BIOTERRORISM
    Judy Lieberman; Fiscal Year: 2007
    ..abstract_text> ..
  4. Engineered Listeria monocytogenes as an AIDS vaccine
    Judy Lieberman; Fiscal Year: 2007
    ..A pregnant mother/neonatal transfer model in macaques will also be used to test for safety and immunogenicity in an animal model that utilizes the animals most vulnerable to Listeria toxicity. ..
  5. Biocontained ImageStream 100 Multispectral Imaging Cytometer
    Judy Lieberman; Fiscal Year: 2007
    ..It will guide efforts for developing effective vaccines and immunotherapies and drug therapies for infection and cancer. ..