Research Topics
Genomes and Genes
| J LiebermanSummaryAffiliation: Harvard University Country: USA Publications
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Publications
Engineered Listeria monocytogenes as an AIDS vaccineJudy Lieberman
The Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
Vaccine 20:2007-10. 2002..Lmdd-gag immunization protects mice from vaccinia-gag challenge and induces mucosal CTL, even after systemic immunization...- The ABCs of granule-mediated cytotoxicity: new weapons in the arsenalJudy Lieberman
Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Rev Immunol 3:361-70. 2003..In this review, I discuss recent insights into the mechanisms of granule-mediated cytotoxicity, focusing on how granzymes A, B and C and granulysin activate cell death through caspase-independent pathways... - Expansion of CD57 and CD62L-CD45RA+ CD8 T lymphocytes correlates with reduced viral plasma RNA after primary HIV infectionJ Lieberman
The Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
AIDS 13:891-9. 1999..We examined whether other immunological parameters might influence the viral setpoint... - Interfering with disease: opportunities and roadblocks to harnessing RNA interferenceJudy Lieberman
Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Trends Mol Med 9:397-403. 2003..RNAi could provide an exciting new therapeutic modality for treating infection, cancer, neurodegenerative disease and other illnesses... - Tracking the killers: how should we measure CD8 T cells in HIV infection?Judy Lieberman
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
AIDS 18:1489-93. 2004 - Avoiding the kiss of death: how HIV and other chronic viruses surviveJudy Lieberman
Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
Curr Opin Immunol 14:478-86. 2002..The chance to eradicate an infection by T cell mediated lysis is undermined after an infection becomes established, in part because the effector immune response is impaired in the setting of chronic antigen... - Dressed to kill? A review of why antiviral CD8 T lymphocytes fail to prevent progressive immunodeficiency in HIV-1 infectionJ Lieberman
Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
Blood 98:1667-77. 2001..Functional defects are qualitatively different and more severe with advanced disease, when interferon gamma production also becomes compromised... - Induction of rapid histone degradation by the cytotoxic T lymphocyte protease Granzyme AD Zhang
Center for Blood Research, Massachusetts General Hospital and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 276:3683-90. 2001..Histone digestion provides a mechanism for unfolding compacted chromatin and facilitating endogenous DNase access to DNA during T cell and natural killer cell granule-mediated apoptosis... - Impaired function of circulating HIV-specific CD8(+) T cells in chronic human immunodeficiency virus infectionP Shankar
Center for Blood Research, Harvard Medical School, and the New England Medical Center, Tufts University School of Medicine, Boston, MA, USA
Blood 96:3094-101. 2000..These results suggest that a significant proportion of HIV-specific CD8 T cells may be functionally compromised in vivo and that some function can be restored by exposure to IL-2... 
Effector differentiation is not prerequisite for generation of memory cytotoxic T lymphocytesN Manjunath
The Center for Blood Research, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
J Clin Invest 108:871-8. 2001..In addition to providing a simple means to generate memory cells in virtually unlimited numbers, our results suggest that effector differentiation is not a prerequisite for memory cell generation...- gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repairDipanjan Chowdhury
CBR Institute for Biomedical Research and The Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell 20:801-9. 2005..The effect of PP2A on gamma-H2AX levels is independent of ATM, ATR, or DNA-PK activity... - Granzyme A activates an endoplasmic reticulum-associated caspase-independent nuclease to induce single-stranded DNA nicksP J Beresford
Center for Blood Research and the Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 276:43285-93. 2001..Thus, GzmA activates a DNase (GzmA-activated DNase) within the SET complex to produce a novel form of DNA damage during cytotoxic T lymphocyte-mediated death... - miR-24-mediated downregulation of H2AX suppresses DNA repair in terminally differentiated blood cellsAshish Lal
Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
Nat Struct Mol Biol 16:492-8. 2009..Therefore, miR-24 upregulation in postreplicative cells reduces H2AX and makes them vulnerable to DNA damage... - Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitorZusen Fan
Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Cell 112:659-72. 2003..GzmA-treated cells with silenced NM23-H1 expression are resistant to GzmA-mediated DNA damage and cytolysis, while cells overexpressing NM23-H1 are more sensitive... - A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recoveryMichael Christopher Keogh
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 439:497-501. 2006..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint... - CD8 T cells specific for human immunodeficiency virus, Epstein-Barr virus, and cytomegalovirus lack molecules for homing to lymphoid sites of infectionG Chen
Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA, USA
Blood 98:156-64. 2001..Lack of CD45RA expression may indicate incomplete differentiation of HIV-specific CD8 T cells to cytotoxic T cells... - Genetically modified anthrax lethal toxin safely delivers whole HIV protein antigens into the cytosol to induce T cell immunityY Lu
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 97:8027-32. 2000..These results lay the foundation for future clinical vaccine studies... - Knocking down disease with siRNAsDerek M Dykxhoorn
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Cell 126:231-5. 2006..This Essay will examine the opportunities for harnessing RNA interference (RNAi) for therapy, as well as the obstacles and possible ways to circumvent them... - Live attenuated Listeria monocytogenes expressing HIV Gag: immunogenicity in rhesus monkeysShisong Jiang
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
Vaccine 25:7470-9. 2007.... - HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme AZusen Fan
Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell Biol 22:2810-20. 2002.... - Cleaving the oxidative repair protein Ape1 enhances cell death mediated by granzyme AZusen Fan
Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
Nat Immunol 4:145-53. 2003..In so doing, GzmA may block cellular repair and force apoptosis. In support of this, cells with silenced Ape1 expression are more sensitive, whereas cells overexpressing noncleavable Ape1 are more resistant, to GzmA-mediated death... - Lentiviral delivery of short hairpin RNAs protects CD4 T cells from multiple clades and primary isolates of HIVSang Kyung Lee
The CBR Institute for Biomedical Research, 800 Huntington Ave, Boston, MA 02115, USA
Blood 106:818-26. 2005..Thus, although 1 or 2 mutations at peripheral sites are tolerated, mutations in the central target cleavage region abolish RNAi activity... - The gp49B1 inhibitory receptor regulates the IFN-gamma responses of T cells and NK cellsXiaogang Gu
Center for Blood Research and Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
J Immunol 170:4095-101. 2003..We conclude that gp49B1 is a unique inhibitory receptor that is induced in multiple lineages of innate and adaptive immune cells during an infection and controls their IFN-gamma, but not cytotoxic responses... - Most antiviral CD8 T cells during chronic viral infection do not express high levels of perforin and are not directly cytotoxicDong Zhang
Center for Blood Research, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Blood 101:226-35. 2003..Impaired antiviral cytotoxicity during chronic infection is not specific to HIV but likely represents the immune response to chronic antigenic exposure... - Granzyme A, which causes single-stranded DNA damage, targets the double-strand break repair protein Ku70Pengcheng Zhu
The CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
EMBO Rep 7:431-7. 2006..Therefore, Ku70 has other antiapoptotic functions in GzmA-induced cell death, which are blocked when GzmA proteolyses Ku70... - Cross-clade CD8 T-cell responses to HIV(IIIB) and Chinese B' and C/B' viruses in North American and Chinese HIV-seropositive donorsVanessa François-Bongarçon
CBR Institute for Biomedical Research, Boston, Massachusetts 02115, USA
J Acquir Immune Defic Syndr 37:1435-44. 2004..T cells, selected for IFN-gamma production to IIIB virus, also efficiently lysed cells infected with Chinese viruses. Therefore, cross-clade CD8 T-cell responses to IIIB virus and prevalent Chinese viral strains are common... - Focal adhesion kinase is required for CXCL12-induced chemotactic and pro-adhesive responses in hematopoietic precursor cellsA M Glodek
Department of Pathology, Joint Program in Transfusion Medicine, Children s Hospital Boston, Boston, MA, USA
Leukemia 21:1723-32. 2007..These studies suggest that FAK may function as a key intermediary in signaling pathways controlling hematopoietic cell lodgment and lineage development... - Granzymes A and B directly cleave lamins and disrupt the nuclear lamina during granule-mediated cytolysisD Zhang
Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 98:5746-51. 2001..Lamins A/C are direct substrates of GzmA, but not GzmB. GzmA and GzmB therefore directly target critical caspase substrates in caspase-resistant cells... - The silent treatment: siRNAs as small molecule drugsD M Dykxhoorn
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Gene Ther 13:541-52. 2006..This review will discuss progress in developing and testing small RNAi-based drugs and potential obstacles... - The Bcl-2 family pro-apoptotic molecule, BNIP3 regulates activation-induced cell death of effector cytotoxic T lymphocytesJ Wan
The Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, MA 02115, USA
Immunology 110:10-7. 2003..Finally, retroviral transduction of BNIP3 antisense RNA diminished AICD in effector CTL. These results suggest that BNIP3 may play an important role in T-cell homeostasis by regulating effector CTL numbers... - Granzyme A activates another way to dieJudy Lieberman
Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Harvard Medical School, Boston, MA, USA
Immunol Rev 235:93-104. 2010..GzmA also has proinflammatory activity; it activates pro-interleukin-1beta and may also have other proinflammatory effects that remain to be elucidated... - Prior exposure to an attenuated Listeria vaccine does not reduce immunogenicity: pre-clinical assessment of the efficacy of a Listeria vaccine in the induction of immune responses against HIVJames B Whitney
Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA
J Immune Based Ther Vaccines 9:2. 2011..abstract:.. - The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell deathDipanjan Chowdhury
CBR Institute for Biomedical Research, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Cell 23:133-42. 2006..After granzyme A activates NM23-H1 to make single-stranded nicks, TREX1 removes nucleotides from the nicked 3' end to reduce the possibility of repair by rejoining the nicked ends... - Running interference: prospects and obstacles to using small interfering RNAs as small molecule drugsDerek M Dykxhoorn
CBR Institute for Biomedical Research, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
Annu Rev Biomed Eng 8:377-402. 2006..Pilot siRNA clinical studies began just three years after the discovery that RNAi works in mammalian cells. This review discusses recent progress and obstacles to using siRNAs as small molecule drugs... - miR-24 Inhibits cell proliferation by targeting E2F2, MYC, and other cell-cycle genes via binding to "seedless" 3'UTR microRNA recognition elementsAshish Lal
Immune Disease Institute, Children s Hospital Boston, Department of Pediatrics, Harvard Medical School, MA 02115, USA
Mol Cell 35:610-25. 2009..The E2F2 3'UTR lacks a predicted miR-24 recognition element. In fact, miR-24 regulates expression of E2F2, MYC, AURKB, CCNA2, CDC2, CDK4, and FEN1 by recognizing seedless but highly complementary sequences... - NFAT5 regulates HIV-1 in primary monocytes via a highly conserved long terminal repeat siteShahin Ranjbar
CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Pathog 2:e130. 2006..Inhibition of the NFAT5-LTR interaction may thus present a novel therapeutic target to suppress HIV-1 replication and progression of AIDS... - A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replicationDipanjan Chowdhury
Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA
Mol Cell 31:33-46. 2008..Therefore, gamma-H2AX elimination at DNA damage foci is required for DNA damage repair, but accomplishing this task involves distinct phosphatases with potentially overlapping roles... - Death by a thousand cuts: granzyme pathways of programmed cell deathDipanjan Chowdhury
Dana Farber Cancer Institute and Department of Radiation Oncology, Harvard Medical School, Boston, Massachusetts 02115, USA
Annu Rev Immunol 26:389-420. 2008..This review discusses what is known about granzyme-mediated pathways of cell death as well as recent studies that implicate granzymes in immune regulation and extracellular proteolytic functions in inflammation... 
Identification of host proteins required for HIV infection through a functional genomic screenAbraham L Brass
Department of Genetics, Center for Genetics and Genomics, Brigham and Women s Hospital, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
Science 319:921-6. 2008..This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing identify potential targets for therapy...- Nuclear war: the granzyme A-bombJudy Lieberman
Center for Blood Research and Department of Pediatrics, Harvard Medical School, 800 Huntington Avenue, Boston, MA 02115, USA
Curr Opin Immunol 15:553-9. 2003..The novel cell death pathway initiated by granzyme A provides a parallel pathway for apoptosis, important in destroying targets that overexpress bcl-2 or are otherwise invulnerable to the caspases... - The silent revolution: RNA interference as basic biology, research tool, and therapeuticDerek M Dykxhoorn
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
Annu Rev Med 56:401-23. 2005..RNAi may provide an important new therapeutic modality for treating infection, cancer, neurodegenerative disease, and other illnesses, although in vivo delivery of small interfering RNAs into cells remains a significant obstacle... - Increased expression of the natural killer cell inhibitory receptor CD85j/ILT2 on antigen-specific effector CD8 T cells and its impact on CD8 T-cell functionM Nedim Ince
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Immunology 112:531-42. 2004..Thus, ILT2 expression, increased on antiviral CD8 cells in chronic infection, may interfere with protective CD8 T-cell function by suppressing IFN-gamma production... - Silencing viral infectionDerek M Dykxhoorn
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
PLoS Med 3:e242. 2006 - The cytotoxic T lymphocyte protease granzyme A cleaves and inactivates poly(adenosine 5'-diphosphate-ribose) polymerase-1Pengcheng Zhu
Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Blood 114:1205-16. 2009..Disrupting PARP-1, which is also a caspase target, is therefore required for efficient apoptosis by both caspase-independent and caspase-dependent pathways... - Selective gene silencing in activated leukocytes by targeting siRNAs to the integrin lymphocyte function-associated antigen-1Dan Peer
CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:4095-100. 2007.... - Micromanipulating cancer: microRNA-based therapeutics?Fabio Petrocca
Immune Disease Institute, Program in Cellular and Molecular Medicine, Children s Hospital Boston and Department of Pediatrics, Harvard Medical School, Boston, MA, USA
RNA Biol 6:335-40. 2009..This review will explore some of the opportunities and obstacles to harnessing microRNA biology for cancer therapy... - miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53Francisco Navarro
Immune Disease Institute and Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Blood 114:2181-92. 2009..However, in p53-null K562 cells, phorbol esters induce miR-34a expression independently of p53 by activating an alternative phorbol ester-responsive promoter to produce a longer pri-miR-34a transcript... - Delivering the kiss of death: progress on understanding how perforin worksMatthew E Pipkin
CBR Institute for Biomedical Research, Harvard Medical School, Boston MA 02115, USA
Curr Opin Immunol 19:301-8. 2007..Recently, an alternative model has been proposed that involves active target cell collaboration with perforin to deliver granzymes and direct the target cell to an apoptotic, rather than necrotic, death... - Sustained small interfering RNA-mediated human immunodeficiency virus type 1 inhibition in primary macrophagesErwei Song
Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
J Virol 77:7174-81. 2003..Under these circumstances, long-term suppression of HIV replication could be achieved with p24 siRNA. Thus, siRNAs can induce potent and long-lasting HIV inhibition in nondividing cells such as macrophages... - Desperately seeking microRNA targetsMarshall Thomas
Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts, USA
Nat Struct Mol Biol 17:1169-74. 2010..Bioinformatic analysis of over-represented pathways and nodes in protein-DNA interactomes formed from experimental candidate miRNA gene target lists can focus attention on biologically significant target genes... - RNA interference targeting Fas protects mice from fulminant hepatitisErwei Song
Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
Nat Med 9:347-51. 2003..Silencing Fas expression with RNAi holds therapeutic promise to prevent liver injury by protecting hepatocytes from cytotoxicity... - Granzyme B binds to target cells mostly by charge and must be added at the same time as perforin to trigger apoptosisLianfa Shi
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
J Immunol 174:5456-61. 2005..Therefore, PFN must be coendocytosed with GzmB to deliver it into the cytosol... - The prospect of silencing disease using RNA interferencePremlata Shankar
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, Mass 02115, USA
JAMA 293:1367-73. 2005..In this article we discuss the basic mechanism of RNAi and the therapeutic opportunities and obstacles for harnessing RNAi for therapy of human disease... - Three regions of HIV-1 gp160 contain clusters of immunodominant CTL epitopesP Shankar
Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
Immunol Lett 52:23-30. 1996..CTL from four subjects recognized at least three distinct epitopes in a.a 591-600 in the context of A24, B8, B14, and B27. CTL from seven subjects recognized epitopes within a.a. 844-863 restricted by A30, B7, B8 and B35... - Recombinant human granzyme A binds to two putative HLA-associated proteins and cleaves one of themP J Beresford
Center for Blood Research, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 94:9285-90. 1997..PHAP II begins to be degraded within minutes of initiation of cytotoxic T lymphocyte attack. PHAP I and II are candidate participants in the granzyme A pathway of cell-mediated cytotoxicity... - Micromanagers of immune cell fate and functionFabio Petrocca
Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
Adv Immunol 102:227-44. 2009..MicroRNAs (miRNAs) regulate cell fate during development and in response to environmental cues. Here, we review the emerging story of how miRNAs regulate immune cell development and function... - The SET complex acts as a barrier to autointegration of HIV-1Nan Yan
Department of Pediatrics, Immune Disease Institute, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Pathog 5:e1000327. 2009..Therefore, the SET complex facilitates HIV-1 infection by preventing suicidal autointegration... - Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivityMing Chieh Shun
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
J Virol 82:11555-67. 2008..This initial systematic mutagenesis of a PWWP domain identifies amino acid residues critical for chromatin binding function and the consequences of their changes on HIV-1 integration and infection... - Perforin triggers a plasma membrane-repair response that facilitates CTL induction of apoptosisDennis Keefe
The CBR Institute for Biomedical Research, Boston, MA 02115, USA
Immunity 23:249-62. 2005..Thus, the target cell actively participates in determining its own fate during cell-mediated death... - Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptorsErwei Song
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Nat Biotechnol 23:709-17. 2005..Furthermore, an ErbB2 single-chain antibody fused with protamine delivered siRNAs specifically into ErbB2-expressing cancer cells. This study demonstrates the potential for systemic, cell-type specific, antibody-mediated siRNA delivery... - Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometryFariyal Ahmed
Immune Disease Institute, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
J Immunol Methods 347:79-86. 2009..However, CD3epsilon exhibited longer residence times (>8 min) at the synapse than Lck... - Small RNAs guide hematopoietic cell differentiation and functionFrancisco Navarro
Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
J Immunol 184:5939-47. 2010..This review summarizes our current knowledge of how miRNAs function in normal and malignant hematopoiesis and how miRNAs might be applied for disease treatment... - Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell deathDenis Martinvalet
Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Cell 133:681-92. 2008..Target cells expressing a cleavage site mutant of NDUFS3 are resistant to GzmA-mediated cell death but remain sensitive to GzmB... - Defying death--HIV mutation to evade cytotoxic T lymphocytesJudy Lieberman
Center for Blood Research, Boston, MA 02115, USA
N Engl J Med 347:1203-4. 2002 - The cytosolic exonuclease TREX1 inhibits the innate immune response to human immunodeficiency virus type 1Nan Yan
Immune Disease Institute and Program in Cellular and Molecular Medicine, Children s Hospital, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, USA
Nat Immunol 11:1005-13. 2010..HIV-stimulated interferon production in cells deficient in TREX1 did not involve known nucleic acid sensors... - Isolation of cytotoxic T cell and NK granules and purification of their effector proteinsJerome Thiery
Program in Cellular and Molecular Medicine, Children s Hospital and Immune Disease Institute, Boston, Massachusetts, USA
Curr Protoc Cell Biol . 2010..Protocols are also given for purifying the key cytolytic molecules (perforin, granzyme A, granzyme B, and granulysin) from isolated cytotoxic granules by fast protein liquid chromatography... - Durable protection from Herpes Simplex Virus-2 transmission following intravaginal application of siRNAs targeting both a viral and host geneYichao Wu
The Immune Disease Institute and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Cell Host Microbe 5:84-94. 2009..Combining siRNAs targeting a viral and host gene protects mice from HSV-2 for a week, irrespective of the time of challenge. Therefore, intravaginal siRNAs could provide sustained protection against viral transmission... - Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expressionMun Chun Chan
Molecular Cardiology Research Institute, Tufts Medical Center, Boston, MA 02111, USA
EMBO J 29:559-73. 2010..Thus, this study provides a molecular basis for the antagonism between the PDGF and TGFbeta pathways, and its effect on the control of the vSMC phenotype... - The functional CD8 T cell response to HIV becomes type-specific in progressive diseaseSang Kyung Lee
Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
J Clin Invest 110:1339-47. 2002..Therefore, in advanced-stage patients, viral-specific CD8 T cells recognizing consensus epitopes persist from an earlier response but no longer effectively recognize autologous virus... - RNA interference and cancer: endogenous pathways and therapeutic approachesDerek M Dykxhoorn
Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Adv Exp Med Biol 615:299-329. 2008..Possible strategies and obstacles to harnessing RNAi for cancer therapy will also be discussed... - An siRNA-based microbicide protects mice from lethal herpes simplex virus 2 infectionDeborah Palliser
CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 439:89-94. 2006..These results suggest that siRNAs are attractive candidates for the active component of a microbicide designed to prevent viral infection or transmission... - Determinants of specific RNA interference-mediated silencing of human beta-globin alleles differing by a single nucleotide polymorphismDerek M Dykxhoorn
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 103:5953-8. 2006.... - Granzyme A induces caspase-independent mitochondrial damage, a required first step for apoptosisDenis Martinvalet
The CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
Immunity 22:355-70. 2005..Superoxide scavengers also block apoptosis by CTLs expressing GzmA and/or GzmB. Therefore, mitochondrial damage is an essential first step in killer cell granule-mediated pathways of apoptosis... - Vaccinia virus induces strong immunoregulatory cytokine production in healthy human epidermal keratinocytes: a novel strategy for immune evasionLuzheng Liu
Harvard Skin Disease Research Center, Department of Dermatology, Brigham and Women's Hospital, 77 Avenue Louis Pasteur, Suite 672, Boston, MA 02115, USA
J Virol 79:7363-70. 2005..quot;.. - HIV-1 antiviral activity of recombinant natural killer cell enhancing factors, NKEF-A and NKEF-B, members of the peroxiredoxin familyRalf Geiben-Lynn
Partners AIDS Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02129, USA
J Biol Chem 278:1569-74. 2003.... - miR-200 enhances mouse breast cancer cell colonization to form distant metastasesDerek M Dykxhoorn
Department of Pediatrics, Immune Disease Institute, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS ONE 4:e7181. 2009..Because some miRNAs are dysregulated in cancer and affect cellular transformation, tumor formation, and metastasis, we examined whether changes in miRNA expression might explain the differences in metastasis of these cells... - Whither or wither microbicides?Robert M Grant
J David Gladstone Institutes, University of California San Francisco, San Francisco, CA 94518, USA
Science 321:532-4. 2008.... - Public health. A sound rationale needed for phase III HIV-1 vaccine trialsDennis R Burton
Scripps Research Institute, La Jolla, California, USA
Science 303:316. 2004 - siRNA-directed inhibition of HIV-1 infectionCarl D Novina
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Nat Med 8:681-6. 2002..siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections... - Interfering with disease: a progress report on siRNA-based therapeuticsAntonin de Fougerolles
Alnylam Pharmaceuticals Inc, 300 Third Street, Cambridge, Massachusetts 02142, USA
Nat Rev Drug Discov 6:443-53. 2007..We conclude by reviewing the latest clinical experience with RNAi therapeutics... - Proliferation responses to HIVp24 during antiretroviral therapy do not reflect improved immune phenotype or functionChristoph G Lange
Center for AIDS Research, Department of Medicine, Division of Infectious Diseases, Case Western Reserve University, University Hospitals of Cleveland, 2061 Cornell Road, Cleveland, OH 44106, USA
AIDS 18:605-13. 2004.... - Adenovirus protein VII functions throughout early phase and interacts with cellular proteins SET and pp32Yuming Xue
Department of Microbiology, University of Virginia Health System, Charlottesville, VA 22908, USA
J Virol 79:2474-83. 2005..Interestingly, disappearance of the dots required ongoing RNA synthesis but not DNA synthesis. Taken together these data indicate that protein VII has an ongoing role during early phase and the beginning of DNA replication... - let-7 regulates self renewal and tumorigenicity of breast cancer cellsFengyan Yu
Department of Breast Surgery, No 2 Affiliated Hospital, Sun Yat Sen University, Guangzhou 510120, People s Republic of China
Cell 131:1109-23. 2007..Therefore let-7 regulates multiple BT-IC stem cell-like properties by silencing more than one target... - Small interfering RNA targeting Fas protects mice against renal ischemia-reperfusion injuryPeter Hamar
Institute of Pathophysiology, Semmelweis University, Nagyvarad ter 4, Budapest, H 1089, Hungary
Proc Natl Acad Sci U S A 101:14883-8. 2004..This study confirms the importance of Fas-mediated apoptosis in renal ischemia-reperfusion injury. Silencing Fas by systemic or local catheterization holds therapeutic promise to limit ischemia-reperfusion injury... - Your health in the 21st century. Silencing bad genesAnthony Komaroff
Newsweek 145:51-2. 2005 - Listeria-infected myeloid dendritic cells produce IFN-beta, priming T cell activationHanping Feng
CBR Institute for Biomedical Research and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
J Immunol 175:421-32. 2005..Exposure to high concentrations of IFN-beta sensitizes naive T cells for Ag-dependent activation... - A mutation in TREX1 that impairs susceptibility to granzyme A-mediated cell death underlies familial chilblain lupusMin Ae Lee-Kirsch
Klinik fur Kinder und Jugendmedizin, Technische Universitat Dresden, Fetscherstr 74, 01307, Dresden, Germany
J Mol Med (Berl) 85:531-7. 2007..Our findings also warrant further investigation of TREX1 in common forms of lupus erythematosus... - Mapping cross-clade HIV-1 vaccine epitopes using a bioinformatics approachAnne S De Groot
TB HIV Research Lab, Brown University, Providence, RI 02912, USA
Vaccine 21:4486-504. 2003..Putative HIV-1 CTL epitopes were selected from this list using the epitope prediction tool EpiMatrix... - A fragment of anthrax lethal factor delivers proteins to the cytosol without requiring protective antigenNicholas Kushner
Harvard AIDS Institute and Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:6652-7. 2003..Furthermore, these results enable us to propose a modified molecular mechanism of anthrax lethal toxin...
Research Grants
- Apoptosis and ImmunityJudy Lieberman; Fiscal Year: 2002..abstract_text> ..
- Developing RNA Interference for and HIV microbicideJudy Lieberman; Fiscal Year: 2005..test siRNA delivery strategies in the human skin blister model; 3. test siRNAs and siRNA delivery strategies for silencing SIV gag and CCR5 in PBMCs from pig-tailed macaques. ..
- RNA INTERFERENCE AS A WEAPON AGAINST BIOTERRORISMJudy Lieberman; Fiscal Year: 2007..abstract_text> ..
- Engineered Listeria monocytogenes as an AIDS vaccineJudy Lieberman; Fiscal Year: 2007..A pregnant mother/neonatal transfer model in macaques will also be used to test for safety and immunogenicity in an animal model that utilizes the animals most vulnerable to Listeria toxicity. ..
- Biocontained ImageStream 100 Multispectral Imaging CytometerJudy Lieberman; Fiscal Year: 2007..It will guide efforts for developing effective vaccines and immunotherapies and drug therapies for infection and cancer. ..