Wayne I Lencer

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Diarrhea-associated HIV-1 APIs potentiate muscarinic activation of Cl- secretion by T84 cells via prolongation of cytosolic Ca2+ signaling
    Paul A Rufo
    GI Cell Biology, Combined Program in Pediatric Gastroenterology and Nutrition, Children s Hospital, 300 Longwood Ave, Boston, MA 02115, USA
    Am J Physiol Cell Physiol 286:C998-C1008. 2004
  2. ncbi request reprint Raft trafficking of AB5 subunit bacterial toxins
    Wayne I Lencer
    GI Cell Biology, Enders 720, Children s Hospital Boston, the Harvard Digestive Diseases Center, and the Department of Pediatrics, Harvard Medical School, 300 Longwood Ave, Boston, MA 02115, USA
    Biochim Biophys Acta 1746:314-21. 2005
  3. pmc To translocate or not: that is the problem
    Wayne I Lencer
    Department of Medicine, Children s Hospital Boston, Harvard Medical School, MA 02115, USA
    Cell Host Microbe 10:179-80. 2011
  4. pmc Dependence of antibody-mediated presentation of antigen on FcRn
    Shuo Wang Qiao
    Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:9337-42. 2008
  5. pmc Gangliosides that associate with lipid rafts mediate transport of cholera and related toxins from the plasma membrane to endoplasmic reticulm
    Yukako Fujinaga
    GI Cell Biology, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 14:4783-93. 2003
  6. pmc Ca2+-dependent calmodulin binding to FcRn affects immunoglobulin G transport in the transcytotic pathway
    Bonny L Dickinson
    The Research Institute for Children, Children s Hospital, Department of Pediatrics, New Orleans, LA 70118, USA
    Mol Biol Cell 19:414-23. 2008
  7. pmc Neonatal Fc receptor for IgG (FcRn) regulates cross-presentation of IgG immune complexes by CD8-CD11b+ dendritic cells
    Kristi Baker
    Division of Gastroenterology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:9927-32. 2011
  8. pmc The immunologic functions of the neonatal Fc receptor for IgG
    Timo Rath
    Division of Gastroenterology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    J Clin Immunol 33:S9-17. 2013
  9. pmc Bidirectional transepithelial IgG transport by a strongly polarized basolateral membrane Fcgamma-receptor
    Steven M Claypool
    Harvard Medical School, Program in Immunology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Mol Biol Cell 15:1746-59. 2004
  10. ncbi request reprint Human neonatal Fc receptor mediates transport of IgG into luminal secretions for delivery of antigens to mucosal dendritic cells
    Masaru Yoshida
    Gastroenterology Division, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Immunity 20:769-83. 2004

Detail Information

Publications58

  1. ncbi request reprint Diarrhea-associated HIV-1 APIs potentiate muscarinic activation of Cl- secretion by T84 cells via prolongation of cytosolic Ca2+ signaling
    Paul A Rufo
    GI Cell Biology, Combined Program in Pediatric Gastroenterology and Nutrition, Children s Hospital, 300 Longwood Ave, Boston, MA 02115, USA
    Am J Physiol Cell Physiol 286:C998-C1008. 2004
    ..These data show that APIs modulate Ca(2+) signaling in secretory epithelial cells and identify a novel target for treatment of clinically important API side effects...
  2. ncbi request reprint Raft trafficking of AB5 subunit bacterial toxins
    Wayne I Lencer
    GI Cell Biology, Enders 720, Children s Hospital Boston, the Harvard Digestive Diseases Center, and the Department of Pediatrics, Harvard Medical School, 300 Longwood Ave, Boston, MA 02115, USA
    Biochim Biophys Acta 1746:314-21. 2005
    ..The way certain lipids and proteins are clustered in the plane of the membrane to form lipid rafts likely explains how the glycolipids can function as sorting motifs for the toxins...
  3. pmc To translocate or not: that is the problem
    Wayne I Lencer
    Department of Medicine, Children s Hospital Boston, Harvard Medical School, MA 02115, USA
    Cell Host Microbe 10:179-80. 2011
    ..In this issue, Sun et al. (2011) show that BoNT binding to one of its cell surface receptors renders it susceptible to pH-dependent conformational changes required for translocation and cellular toxicity...
  4. pmc Dependence of antibody-mediated presentation of antigen on FcRn
    Shuo Wang Qiao
    Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:9337-42. 2008
    ....
  5. pmc Gangliosides that associate with lipid rafts mediate transport of cholera and related toxins from the plasma membrane to endoplasmic reticulm
    Yukako Fujinaga
    GI Cell Biology, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 14:4783-93. 2003
    ..Our results explain why LTIIb does not cause disease in humans and suggest that gangliosides with high affinity for lipid rafts may provide a general vehicle for the transport of toxins to the ER...
  6. pmc Ca2+-dependent calmodulin binding to FcRn affects immunoglobulin G transport in the transcytotic pathway
    Bonny L Dickinson
    The Research Institute for Children, Children s Hospital, Department of Pediatrics, New Orleans, LA 70118, USA
    Mol Biol Cell 19:414-23. 2008
    ..These results suggest a novel mechanism for regulation of IgG transport by calmodulin-dependent sorting of FcRn and its cargo away from a degradative pathway and into a bidirectional transcytotic route...
  7. pmc Neonatal Fc receptor for IgG (FcRn) regulates cross-presentation of IgG immune complexes by CD8-CD11b+ dendritic cells
    Kristi Baker
    Division of Gastroenterology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:9927-32. 2011
    ..Given the centrality of FcRn in controlling cross-presentation, these studies lay the foundation for a unique means to therapeutically manipulate CD8(+) T-cell responses...
  8. pmc The immunologic functions of the neonatal Fc receptor for IgG
    Timo Rath
    Division of Gastroenterology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    J Clin Immunol 33:S9-17. 2013
    ..The increasing appreciation for FcRn in both homeostatic and pathological conditions is generating an intense interest in the potential for therapeutic modulation of FcRn binding to IgG and albumin...
  9. pmc Bidirectional transepithelial IgG transport by a strongly polarized basolateral membrane Fcgamma-receptor
    Steven M Claypool
    Harvard Medical School, Program in Immunology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Mol Biol Cell 15:1746-59. 2004
    ..Thus, the strong cell surface polarity displayed by hFcRn results from dominant basolateral sorting by motifs in the cytoplasmic tail that nonetheless allows for a cycle of bidirectional transcytosis...
  10. ncbi request reprint Human neonatal Fc receptor mediates transport of IgG into luminal secretions for delivery of antigens to mucosal dendritic cells
    Masaru Yoshida
    Gastroenterology Division, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
    Immunity 20:769-83. 2004
    ..These results explain how IgG is secreted onto mucosal surfaces and scavenges luminal antigens for recognition by the immune system...
  11. pmc Intoxication of zebrafish and mammalian cells by cholera toxin depends on the flotillin/reggie proteins but not Derlin-1 or -2
    David E Saslowsky
    Division of Gastroenterology, Children s Hospital, Boston, Massachusetts, USA
    J Clin Invest 120:4399-4409. 2010
    ..These results revise current models for CT intoxication and implicate protein scaffolding of lipid rafts in the endo-somal sorting of the toxin-GM1 complex...
  12. pmc Selective translocation of the Bordetella pertussis adenylate cyclase toxin across the basolateral membranes of polarized epithelial cells
    Joshua C Eby
    Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 285:10662-70. 2010
    ..Thus, elements of the basolateral membrane render epithelial cells highly sensitive to the entry of ACT in the absence of a specific receptor for toxin binding...
  13. doi request reprint Immune and non-immune functions of the (not so) neonatal Fc receptor, FcRn
    Kristi Baker
    Division of Gastroenterology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Semin Immunopathol 31:223-36. 2009
    ..A greater understanding of FcRn's pleiotropic roles is thus imperative for a variety of therapeutic purposes...
  14. pmc Attenuated endocytosis and toxicity of a mutant cholera toxin with decreased ability to cluster ganglioside GM1 molecules
    Anne A Wolf
    GI Cell Biology, Enders 720, Children s Hospital Boston, 300 Longwood Ave, Boston, MA 02115, USA
    Infect Immun 76:1476-84. 2008
    ....
  15. pmc An FcRn-dependent role for anti-flagellin immunoglobulin G in pathogenesis of colitis in mice
    Kanna Kobayashi
    Gastroenterology Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Gastroenterology 137:1746-56.e1. 2009
    ....
  16. ncbi request reprint Role of p97 AAA-ATPase in the retrotranslocation of the cholera toxin A1 chain, a non-ubiquitinated substrate
    Michael Kothe
    GI Cell Biology, Children s Hospital Boston, the Harvard Digestive Diseases Center, Harvard Medical School, Massachusetts 02115, USA
    J Biol Chem 280:28127-32. 2005
    ..These results suggest that p97 does not provide the primary driving force for extracting the A1 chain from the endoplasmic reticulum, a finding that is consistent with a requirement for polyubiquitination in p97 function...
  17. pmc Cholera toxin: an intracellular journey into the cytosol by way of the endoplasmic reticulum
    Naomi L B Wernick
    GI Cell Biology, Children s Hospital and Harvard Medical School, Boston, MA, USA
    Toxins (Basel) 2:310-25. 2010
    ..Here, we review the mechanisms of toxin trafficking by GM1 and retro-translocation of the A1-chain to the cytosol...
  18. pmc Lipid sorting by ceramide structure from plasma membrane to ER for the cholera toxin receptor ganglioside GM1
    Daniel J F Chinnapen
    Division of Gastroenterology, Boston Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 23:573-86. 2012
    ..The results implicate a protein-dependent mechanism of lipid sorting by ceramide structure and provide a molecular explanation for the diversity and specificity of retrograde trafficking by CT in host cells...
  19. pmc The recycling and transcytotic pathways for IgG transport by FcRn are distinct and display an inherent polarity
    Salit Tzaban
    Children s Hospital, Gastroenterology Division, Harvard Digestive Diseases Center, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 185:673-84. 2009
    ....
  20. pmc Neonatal Fc receptor: from immunity to therapeutics
    Timothy T Kuo
    Division of Gastroenterology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 75 Francis St, Boston, MA 02115, USA
    J Clin Immunol 30:777-89. 2010
    ..Here, we aim to summarize the basic understanding of FcRn biology, its functions in various organs, and the therapeutic design of antibody- and albumin-based therapeutics in light of their interactions with FcRn...
  21. pmc N-terminal extension of the cholera toxin A1-chain causes rapid degradation after retrotranslocation from endoplasmic reticulum to cytosol
    Naomi L B Wernick
    Gastrointestinal Cell Biology Laboratory, Children s Hospital, and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 285:6145-52. 2010
    ..Thus, retrotranslocation and refolding of the wild-type A1-chain must proceed in a way that protects these Lys residues from attack by E3 ligases...
  22. pmc Retrograde transport of cholera toxin from the plasma membrane to the endoplasmic reticulum requires the trans-Golgi network but not the Golgi apparatus in Exo2-treated cells
    Yan Feng
    Department of Cell Biology, Institute of Chemistry and Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    EMBO Rep 5:596-601. 2004
    ..We propose that even in the absence of Exo2 the glycolipid pathway that carries the toxin from plasma membrane into the ER bypasses the Golgi apparatus entirely...
  23. pmc Neonatal Fc receptor for IgG regulates mucosal immune responses to luminal bacteria
    Masaru Yoshida
    Department of Medicine, Brigham and Women s Hospital, and Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115, USA
    J Clin Invest 116:2142-2151. 2006
    ....
  24. ncbi request reprint A biochemical method for tracking cholera toxin transport from plasma membrane to Golgi and endoplasmic reticulum
    Heidi E De Luca
    Gastrointestinal Cell Biology, Children s Hospital Boston, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 341:127-39. 2006
    ..This provides a biochemical readout for toxin entry into the trans Golgi (by 35S-sulfation) and ER (by N-glycosylation). In this chapter, we describe the methods we developed to study this trafficking pathway...
  25. pmc Cross-presentation of IgG-containing immune complexes
    Kristi Baker
    Division of Gastroenterology, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Cell Mol Life Sci 70:1319-34. 2013
    ....
  26. pmc Ceramide activates JNK to inhibit a cAMP-gated K+ conductance and Cl- secretion in intestinal epithelia
    David E Saslowsky
    GI Cell Biology, Children s Hospital, and the Harvard Digestive Diseases Center, Boston, MA 02115, USA
    FASEB J 23:259-70. 2009
    ..These results define a novel lipid-mediated pathway for regulation of salt and water homeostasis at mucosal surfaces...
  27. pmc Distribution of the IgG Fc receptor, FcRn, in the human fetal intestine
    Uzma Shah
    Mucosal Immunology Laboratory, Combined Program in Pediatric Gastroenterology and Nutrition, Massachusetts General Hospital, Charlestown, Massachusetts 02129 4404, USA
    Pediatr Res 53:295-301. 2003
    ....
  28. ncbi request reprint The intracellular voyage of cholera toxin: going retro
    Wayne I Lencer
    GI Cell Biology, Children s Hospital and the Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Trends Biochem Sci 28:639-45. 2003
    ..Upon entering the cytosol, the A1-chain rapidly refolds, avoids the proteasome and induces toxicity...
  29. pmc Cholera toxin toxicity does not require functional Arf6- and dynamin-dependent endocytic pathways
    Ramiro H Massol
    Department of Cell Biology, Harvard Medical School and The Center for Blood Research for Biomedical Research, Boston, Massachusetts 02115, USA
    Mol Biol Cell 15:3631-41. 2004
    ..These results are consistent with the existence of an additional retrograde pathway used by CT to reach the ER...
  30. pmc Rafting with cholera toxin: endocytosis and trafficking from plasma membrane to ER
    Daniel J F Chinnapen
    GI Cell Biology, Children s Hospital, Boston, MA 21115, USA
    FEMS Microbiol Lett 266:129-37. 2007
    ..GM1 is the vehicle that carries CT from PM to ER. Thus, the toxin pathway from PM to ER is a lipid-based sorting pathway, which is potentially meditated by the determinants of the GM1 ganglioside structure itself...
  31. ncbi request reprint Retrograde transport of cholera toxin into the ER of host cells
    Wayne I Lencer
    GI Cell Biology, Children s Hospital Boston, Harvard Digestive Diseases Center, Department of Pediatrics Harvard Medical School, Boston, MA 02115, USA
    Int J Med Microbiol 293:491-4. 2004
    ..Here we discuss recent studies on the mechanism of transport from plasma membrane to the ER and on the reactions that unfold and retrotranslocate a portion of the toxin to the cytosol where toxicity is induced...
  32. ncbi request reprint Uncoupling of the cholera toxin-G(M1) ganglioside receptor complex from endocytosis, retrograde Golgi trafficking, and downstream signal transduction by depletion of membrane cholesterol
    Anne A Wolf
    Gastrointestinal Cell Biology, Department of Pediatrics, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:16249-56. 2002
    ..Taken together these data imply that cholesterol may function to couple the CT-G(M1) complex with raft domains and with other membrane components of the lipid raft required for CT entry into the cell...
  33. pmc Receptor-mediated immunoglobulin G transport across mucosal barriers in adult life: functional expression of FcRn in the mammalian lung
    Gerburg M Spiekermann
    The Combined Program in Pediatric Gastroenterology and Nutrition, Children s Hospital, Boston, MA 02115, USA
    J Exp Med 196:303-10. 2002
    ..These data show that mucosal surfaces that express FcRn reabsorb IgG and explain a mechanism by which IgG may act in immune surveillance to retrieve lumenal antigens for processing in the lamina propria or systemically...
  34. pmc The heavy chain of neonatal Fc receptor for IgG is sequestered in endoplasmic reticulum by forming oligomers in the absence of beta2-microglobulin association
    Xiaoping Zhu
    Gastroenterology Division, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Biochem J 367:703-14. 2002
    ..The beta(2)m association with FcRn HC is critical for correct folding of FcRn and exiting the ER for routing to endosomes and the cell surface...
  35. ncbi request reprint A passionate kiss, then run: exocytosis and recycling of IgG by FcRn
    Wayne I Lencer
    GI Cell Biology, Division of Pediatric Gastroenterology and Nutrition, Children s Hospital, Boston, MA 02115, USA
    Trends Cell Biol 15:5-9. 2005
    ..Similar to the process seen for neurotransmitter release at synaptic junctions, other vesicles fuse only partially, releasing FcRn-IgG complexes to mix into the plasma membrane in cycles of 3-4s over prolonged periods of time...
  36. pmc TorsinA participates in endoplasmic reticulum-associated degradation
    Flavia C Nery
    1 Neuroscience Center, Department of Neurology, and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Program in Neuroscience, Harvard Medical School, Boston, Massachusetts 02114, USA 2
    Nat Commun 2:393. 2011
    ..Therefore, compromised ERAD function in the cells of DYT1 patients may increase sensitivity to endoplasmic reticulum stress with consequent alterations in neuronal function contributing to the disease state...
  37. ncbi request reprint Conversion of apical plasma membrane sphingomyelin to ceramide attenuates the intoxication of host cells by cholera toxin
    David E Saslowsky
    Children s Hospital, Harvard Digestive Diseases Center, Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA
    Cell Microbiol 10:67-80. 2008
    ..This result suggests that the cause for toxin resistance occurs at steps required for retro-translocation of the CT A1-chain to the cytosol...
  38. pmc How the controller is controlled - neonatal Fc receptor expression and immunoglobulin G homeostasis
    Shuo Wang Qiao
    Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Immunology 120:145-7. 2007
  39. pmc The Cdc42 inhibitor secramine B prevents cAMP-induced K+ conductance in intestinal epithelial cells
    Henry E Pelish
    Department of Cell Biology and The CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts, USA
    Biochem Pharmacol 71:1720-6. 2006
    ..These results suggest that Rho GTPases may be involved in the regulation of the chloride secretory response and identify secramine B an inhibitor of cAMP-dependent K+ conductance in intestinal epithelial cells...
  40. pmc Insights on the trafficking and retro-translocation of glycosphingolipid-binding bacterial toxins
    Jin A Cho
    Division of Gastroenterology and Nutrition, Department of Medicine, Children s Hospital Boston, Boston MA, USA Department of Pediatrics, Harvard Medical School, Boston, MA, USA
    Front Cell Infect Microbiol 2:51. 2012
    ..We also discuss several new studies that address the mechanisms of toxin unfolding in the ER and the mechanisms of CT A1-chain retro-translocation to the cytosol...
  41. pmc Functional reconstitution of human FcRn in Madin-Darby canine kidney cells requires co-expressed human beta 2-microglobulin
    Steven M Claypool
    Harvard Medical School, Program in Immunology, Gastroenterogy Division, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 277:28038-50. 2002
    ..These results show that human FcRn travels to the cell surface via the normal secretory pathway and that the appropriate expression and function of human FcRn in MDCK cells depends upon the co-expression of human beta(2)m...
  42. pmc Role of ubiquitination in retro-translocation of cholera toxin and escape of cytosolic degradation
    Chiara Rodighiero
    GI Cell Biology, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    EMBO Rep 3:1222-7. 2002
    ....
  43. pmc The unfolded protein response element IRE1α senses bacterial proteins invading the ER to activate RIG-I and innate immune signaling
    Jin A Cho
    Department of Medicine, Division of GI Cell Biology, Boston Children s Hospital, Boston, MA 02115, USA
    Cell Host Microbe 13:558-69. 2013
    ..We propose IRE1α provides for a generalized mechanism of innate immune surveillance originating within the ER lumen...
  44. pmc Multivalent immune complexes divert FcRn to lysosomes by exclusion from recycling sorting tubules
    Andrew W Weflen
    Division of Gastroenterology, Boston Children s Hospital, Boston, MA 02115 Department of Pediatrics, Harvard Medical School, Boston, MA 02115 Harvard Digestive Diseases Center, Boston, MA 02115 Division of Gastroenterology, Brigham and Women s Hospital, Boston, MA 02115 Department of Biomedical Science, University of Amsterdam, 1012 WX Amsterdam, Netherlands College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China
    Mol Biol Cell 24:2398-405. 2013
    ..Thus physical properties of the lumenal ligand-receptor complex appear to act as key determinants for sorting between the recycling and lysosomal pathways by regulating FcRn entry into recycling tubules. ..
  45. ncbi request reprint Claudin-8 expression in Madin-Darby canine kidney cells augments the paracellular barrier to cation permeation
    Alan S L Yu
    Department of Medicine, University of Southern California Keck School of Medicine, Los Angeles, California 90033, USA
    J Biol Chem 278:17350-9. 2003
    ..Our findings suggest that claudin-8 plays an important role in the paracellular cation barrier of the distal renal tubule...
  46. ncbi request reprint Paneth cell cryptdins act in vitro as apical paracrine regulators of the innate inflammatory response
    Patricia W Lin
    Gastrointestinal Cell Biology Department, Combined Program in Pediatric Gastroenterology and Nutrition, Children s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    J Biol Chem 279:19902-7. 2004
    ..These results show that selective cryptdins may amplify their roles in innate immunity by acting as novel paracrine agonists to coordinate an inflammatory response with the antimicrobial secretions of Paneth cells...
  47. pmc The zebrafish dag1 mutant: a novel genetic model for dystroglycanopathies
    Vandana Gupta
    Genomics Program and Division of Genetics, The Manton Center for Orphan Disease Research, Children s Hospital Boston, Boston, MA 02115, USA
    Hum Mol Genet 20:1712-25. 2011
    ....
  48. ncbi request reprint Signal transduction by bacterial proteins
    Wayne I Lencer
    Children s Hospital, Boston, Massachusetts, USA
    J Pediatr Gastroenterol Nutr 40:S33-4. 2005
  49. pmc Patching a leaky intestine
    Wayne I Lencer
    Department of Medicine, Children s Hospital Boston and Harvard Medical School, Boston, USA
    N Engl J Med 359:526-8. 2008
  50. pmc Derlin-1 facilitates the retro-translocation of cholera toxin
    Kaleena M Bernardi
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    Mol Biol Cell 19:877-84. 2008
    ..CTB may play a role in this process by targeting the holotoxin to Derlin-1, enabling the Derlin-1-bound PDI to unfold the A1 subunit and prepare it for transport...
  51. pmc Protein disulfide isomerase-like proteins play opposing roles during retrotranslocation
    Michele L Forster
    Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA
    J Cell Biol 173:853-9. 2006
    ..Thus, our data identify PDI family proteins that play opposing roles in ER quality control and establish an assay to further delineate the mechanism of CT retrotranslocation...
  52. ncbi request reprint Trafficking of cholera toxin-ganglioside GM1 complex into Golgi and induction of toxicity depend on actin cytoskeleton
    Kamran Badizadegan
    Department of Pathology, Massachusetts General Hospital, Boston 02114, USA
    Am J Physiol Cell Physiol 287:C1453-62. 2004
    ....
  53. ncbi request reprint Research agenda for pediatric gastroenterology, hepatology and nutrition: secretion and diarrhea. Report of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition for the Children's Digestive Health and Nutrition Foundation
    Wayne I Lencer
    Children s Digestive Health and Nutrition Foundation, PO Box 6, Flourtown, PA 19031, USA
    J Pediatr Gastroenterol Nutr 35:S246-9. 2002
  54. pmc Pulmonary delivery of an erythropoietin Fc fusion protein in non-human primates through an immunoglobulin transport pathway
    Alan J Bitonti
    Syntonix Pharmaceuticals, Incorporated, Waltham, MA 02451, USA
    Proc Natl Acad Sci U S A 101:9763-8. 2004
    ..c. in humans. These studies show that FcRn can be harnessed to noninvasively deliver bioactive proteins into the systemic circulation in therapeutic quantities...
  55. ncbi request reprint Myosin light chain phosphorylation regulates barrier function by remodeling tight junction structure
    Le Shen
    Department of Pathology, The University of Chicago, 5841 South Maryland Avenue, MC 1089, Chicago, IL 60637, USA
    J Cell Sci 119:2095-106. 2006
    ..We conclude that myosin light chain phosphorylation alone is sufficient to induce tight junction regulation and provide new insights into the molecular mechanisms that mediate this regulation...
  56. ncbi request reprint Cryptdin 3 forms anion selective channels in cytoplasmic membranes of human embryonic kidney cells
    Gang Yue
    Center for Cell and Molecular Signaling, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    Am J Physiol Gastrointest Liver Physiol 282:G757-65. 2002
    ..1, 1.6, 4.2, and 12.5 ms. These results suggest that cryptdin 3 forms anion-selective channels on the cytoplasmic membrane of HEK cells and that the kinetics of one such channel are affected by its interaction with other such channels...
  57. ncbi request reprint Calnexin and ERp57 facilitate the assembly of the neonatal Fc receptor for IgG with beta 2-microglobulin in the endoplasmic reticulum
    Xiaoping Zhu
    Laboratory of Immunology, Virginia Maryland Regional College of Veterinary Medicine, University of Maryland, 8075 Greenmead Drive, College Park, MD 20742, USA
    J Immunol 175:967-76. 2005
    ..220 (tapasin-deficient) and 721.174 (TAP-deficient) cells transfected with FcRn. These findings show the importance of chaperones in FcRn assembly...
  58. ncbi request reprint The HA proteins of botulinum toxin disrupt intestinal epithelial intercellular junctions to increase toxin absorption
    Takuhiro Matsumura
    Laboratory for Infection Cell Biology, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamada oka, Suita, Osaka 565 0871, Japan
    Cell Microbiol 10:355-64. 2008
    ..These studies show that the 16S BoNT complex is a multifunctional protein assembly equipped with the machinery to efficiently breach the intestinal barrier and act systemically on peripheral nerves...

Research Grants25

  1. TRAINING IN PEDIATRIC GASTROENTEROLOGY AND NUTRITION
    Wayne Lencer; Fiscal Year: 2007
    ..Integration of training with the candidates' future goals in academic medicine, a strong focus on original research, and strong mentoring in science and career development are essential features of the program. ..
  2. FcRn-dependent sorting of IgG and IgG-opsinized antigens by epithelial cells
    Wayne Lencer; Fiscal Year: 2009
    ..Here, we study how an intestinal receptor for transporting immunoglobulin G (the most dominant form of antibodies in humans) dictates the outcome of this interaction. ..
  3. FcRn-dependent sorting of IgG and IgG-opsinized antigens by epithelial cells
    Wayne Lencer; Fiscal Year: 2010
    ..Here, we study how an intestinal receptor for transporting immunoglobulin G (the most dominant form of antibodies in humans) dictates the outcome of this interaction. ..
  4. Intestinal Disease - enterocyte toxin interaction
    Wayne Lencer; Fiscal Year: 2007
    ..Such diseases are global in distribution and include acute infectious diarrheas as well as those that result from abnormal interactions with the intestinal microflora, such as IBD. ..
  5. Intestinal Disease - enterocyte toxin interaction
    Wayne Lencer; Fiscal Year: 2010
    ..Such diseases are global in distribution and include acute infectious diarrheas as well as those that result from abnormal interactions with the intestinal microflora, such as IBD. ..
  6. Intestinal Disease: Enterocyte/Toxin Interaction
    Wayne Lencer; Fiscal Year: 2005
    ..abstract_text> ..
  7. INTESTINAL DISEASE--ENTEROCYTE TOXIN INTERACTION
    Wayne Lencer; Fiscal Year: 2000
    ..Such information may enhance the development of novel strategies for oral immunization and tissue specific drug delivery for use in a variety of intestinal diseases. ..
  8. CRYPTDIN EFFECTS ON CRYPT EPITHELIA
    Wayne Lencer; Fiscal Year: 2004
    ....
  9. Genetic Analysis for Cell Invasion by Bacterial Toxins
    Wayne Lencer; Fiscal Year: 2004
    ..The project has direct relevance to the fields of cell biology, microbial pathogenesis, and biodefense, and it has clinical application. ..