Research Topics
Genomes and Genes
| M D LayneSummaryAffiliation: Harvard University Country: USA Publications
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Detail Information
Publications
Impaired abdominal wall development and deficient wound healing in mice lacking aortic carboxypeptidase-like proteinM D Layne
Cardiovascular, Boston, Massachusetts 02115, USA
Mol Cell Biol 21:5256-61. 2001..5-day-postconception embryos exhibited a reduced proliferative capacity compared with wild-type cells. These results indicate that ACLP is an ECM protein that is essential for embryonic development and dermal wound healing processes...
Characterization of the mouse aortic carboxypeptidase-like protein promoter reveals activity in differentiated and dedifferentiated vascular smooth muscle cellsMatthew D Layne
Pulmonary and Critical Care, Divisions and the Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts, USA
Circ Res 90:728-36. 2002..The ACLP promoter may provide a novel tool to target gene expression to dedifferentiated VSMCs...- Role of macrophage-expressed adipocyte fatty acid binding protein in the development of accelerated atherosclerosis in hypercholesterolemic miceM D Layne
Program of Developmental Cardiovascular Biology, Cardiovascular Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
FASEB J 15:2733-5. 2001..Taken together, we demonstrate the importance of macrophage aP2 in the development of atherosclerotic lesions... - Cardiovascular basic helix loop helix factor 1, a novel transcriptional repressor expressed preferentially in the developing and adult cardiovascular systemM T Chin
Program of Developmental Cardiovascular Biology, Cardiovascular Program of Developmental Cardiovascular Biology, Cardiology Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02115, USA
J Biol Chem 275:6381-7. 2000..Our data suggest that CHF1 functions as a transcriptional repressor and may play an important role in cardiovascular development... - Upstream stimulatory factors regulate aortic preferentially expressed gene-1 expression in vascular smooth muscle cellsY H Chen
Pulmonary and Critical Care and Cardiovascular Divisions, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, USA
J Biol Chem 276:47658-63. 2001..Our studies demonstrate that USF factors contribute to the regulation of APEG-1 expression and may influence the differentiation of VSMC... - Aortic carboxypeptidase-like protein, a novel protein with discoidin and carboxypeptidase-like domains, is up-regulated during vascular smooth muscle cell differentiationM D Layne
Cardiovascular Biology Laboratory, Harvard School of Public Health, Boston, Massachusetts 02115, USA
J Biol Chem 273:15654-60. 1998..These results indicate that ACLP may play a role in differentiated vascular smooth muscle cells... - CLIF, a novel cycle-like factor, regulates the circadian oscillation of plasminogen activator inhibitor-1 gene expressionK Maemura
Cardiovascular and the Pulmonary and Critical Care Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 275:36847-51. 2000..These results suggest that CLIF regulates the circadian oscillation of PAI-1 gene expression in endothelial cells. In addition, the results potentially provide a molecular basis for the morning onset of myocardial infarction... - Molecular cloning, characterization, and promoter analysis of the mouse Crp2/SmLim gene. Preferential expression of its promoter in the vascular smooth muscle cells of transgenic miceS F Yet
Cardiovascular Biology Laboratory, Harvard School of Public Health, Boston, Massachusetts 02115, USA
J Biol Chem 273:10530-7. 1998..In vivo, however, the 5-kilobase fragment directed high level expression of the lacZ reporter gene preferentially in the VSMC of transgenic mice, indicating the presence of VSMC-specific element(s) in this fragment... - Embryonic expression suggests an important role for CRP2/SmLIM in the developing cardiovascular systemM K Jain
Cardiovascular Biology Laboratory, Harvard School of Public Health, The Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
Circ Res 83:980-5. 1998..These patterns of expression were maintained in the adult animal and suggest an important role for this gene family in the development of smooth and striated muscle... - Akt participation in the Wnt signaling pathway through DishevelledS Fukumoto
Cardiovascular and Pulmonary and Critical Care Divisions, Department of Medicine, Brigham and Women's Hospital and the Cardiovascular Research Center Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 276:17479-83. 2001..Therefore, Akt acts in association with Dvl as an important regulator of the Wnt signaling pathway... - Cardiac-specific expression of heme oxygenase-1 protects against ischemia and reperfusion injury in transgenic miceS F Yet
Cardiovascular and Pulmonary and Critical Care, Division and the Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts, USA
Circ Res 89:168-73. 2001..Our data demonstrate that overexpression of HO-1 in the cardiomyocyte protects against ischemia and reperfusion injury, thus improving the recovery of cardiac function... - Regulation of myogenic terminal differentiation by the hairy-related transcription factor CHF2J Sun
Cardiovascular Division, Brigham and Women s Hospital, Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 276:18591-6. 2001..Our data indicate that CHF2 functions as a transcriptional repressor of myogenesis by formation of an inactive heterodimeric complex with MyoD and likely plays an important role in muscle development... - Striated muscle preferentially expressed genes alpha and beta are two serine/threonine protein kinases derived from the same gene as the aortic preferentially expressed gene-1C M Hsieh
Cardiovascular and the Pulmonary and Critical Care Divisions, Brigham and Women s Hospital, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 275:36966-73. 2000..This developmental regulation suggests that both SPEGalpha and SPEGbeta can serve as sensitive markers for striated muscle differentiation and that they may be important for adult striated muscle function... - Absence of adipocyte fatty acid binding protein prevents the development of accelerated atherosclerosis in hypercholesterolemic miceM A Perrella
Program of Developmental Cardiovascular Biology, Cardiovascular Division, and. Pulmonary and Critical Care Division, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA
FASEB J 15:1774-6. 2001 - Genomic cloning and promoter analysis of aortic preferentially expressed gene-1. Identification of a vascular smooth muscle-specific promoter mediated by an E box motifC M Hsieh
Cardiovascular Biology Laboratory, Harvard School of Public Health, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
J Biol Chem 274:14344-51. 1999..To our knowledge, this is the first demonstration of an E box motif that mediates gene expression restricted to VSMC... - Exacerbation of chronic renovascular hypertension and acute renal failure in heme oxygenase-1-deficient miceP Wiesel
Program of Developmental Cardiovascular Biology, Brigham and Women s Hospital, Boston, MA 02115, USA
Circ Res 88:1088-94. 2001..Moreover, renal artery clipping leads to an acute increase in ischemic damage and death in the absence of HO-1... - Human EZF, a Krüppel-like zinc finger protein, is expressed in vascular endothelial cells and contains transcriptional activation and repression domainsS F Yet
Cardiovascular Biology Laboratory, Harvard School of Public Health, Boston, Massachusetts 02115, USA
J Biol Chem 273:1026-31. 1998..By fusing hEZF to the DNA-binding domain of GAL4, we mapped a repression domain in hEZF to amino acids 181-388. We also found that amino acids 91-117 of hEZF confer an activation function on the GAL4 DNA-binding domain... - Hypoxia induces severe right ventricular dilatation and infarction in heme oxygenase-1 null miceS F Yet
Cardiovascular Biology Laboratory, Harvard School of Public Health, Boston, Massachusetts 02115, USA
J Clin Invest 103:R23-9. 1999..Our data suggest that in the absence of HO-1, cardiomyocytes have a maladaptive response to hypoxia and subsequent pulmonary hypertension. J.Clin. Invest. 103:R23-R29 (1999)... - Pre-emptive gene therapy using recombinant adeno-associated virus delivery of extracellular superoxide dismutase protects heart against ischemic reperfusion injury, improves ventricular function and prolongs survivalR S Agrawal
Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, MA 02115, USA
Gene Ther 11:962-9. 2004..This concept of preinjury delivery and 'pre-emptive' gene therapy via the expression of a secreted protein that renders paracrine therapeutic action can be an effective strategy for organ protection against future injury... - Absence of heme oxygenase-1 exacerbates atherosclerotic lesion formation and vascular remodelingShaw Fang Yet
Pulmonary and Critical Care Division, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
FASEB J 17:1759-61. 2003..VSMC isolated from HO-1-/- mice were susceptible to oxidant stress, leading to cell death. Our data demonstrate that HO-1 plays an essential protective role in the pathophysiology of atherosclerosis and vein graft stenosis... - Discoidin domain receptor 2 impairs insulin-stimulated insulin receptor substrate-1 tyrosine phosphorylation and glucose uptake in 3T3-L1 adipocytesH Zurakowski
Department of Medicine, University of Ottawa, Ottawa Health Research Institute, Ottawa, ON K1Y 4E9 Canada, and Pulmonary Division, Brigham and Women s Hospital, Boston, MA, USA
Horm Metab Res 39:575-81. 2007..05 for both). Our data show that DDR2 is expressed in adipose cells and that its overexpression leads to insulin resistance... - Regulation of smooth muscle cell differentiation by AT-rich interaction domain transcription factors Mrf2alpha and Mrf2betaMasafumi Watanabe
Cardiovascular Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Mass 02115, USA
Circ Res 91:382-9. 2002..Furthermore, Mrf2alpha and Mrf2beta retarded cellular proliferation. These data implicate Mrf2 as a novel regulator of smooth muscle cell differentiation and proliferation... - Absence of heme oxygenase-1 exacerbates myocardial ischemia/reperfusion injury in diabetic miceXiaoli Liu
Pulmonary and Critical Care Division, Brigham and Women s Hospital, 75 Francis St, Thorn 932A, Boston, MA 02115, USA
Diabetes 54:778-84. 2005..Our data demonstrate that the absence of HO-1 renders animals more susceptible to myocardial ischemia/reperfusion damage and diabetes worsens the injury... - Reduction of nitric oxide synthase 2 expression by distamycin A improves survival from endotoxemiaRebecca M Baron
Division of Pulmonary and Critical Care Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
J Immunol 173:4147-53. 2004..Our data point to a novel approach in modulating NOS2 expression in vivo during endotoxemia and suggest the potential for alternative treatment approaches for critical illness... - Endotoxin-induced down-regulation of Elk-3 facilitates heme oxygenase-1 induction in macrophagesSu Wol Chung
Pulmonary and Critical Care Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
J Immunol 176:2414-20. 2006..Taken together, our data reveal that Elk-3 serves as an important repressor of HO-1 gene transcription and contributes to the tight control of HO-1 gene regulation in the setting of inflammatory stimuli... - Aortic carboxypeptidase-like protein is expressed in collagen-rich tissues during mouse embryonic developmentBonna Ith
Pulmonary and Critical Care Division, Department of Medicine, Brigham and Women s Hospital, 75 Francis Street TH932A, Boston, MA 02115, USA
Gene Expr Patterns 5:533-7. 2005..Overall, the developmental expression pattern is consistent with a regulatory or structural role in the abdominal wall, vasculature, and dermis... - Aortic carboxypeptidase-like protein is expressed in fibrotic human lung and its absence protects against bleomycin-induced lung fibrosisSCOTT L SCHISSEL
Division of Pulmonary and Critical Care Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
Am J Pathol 174:818-28. 2009..Therefore, both ACLP and its discoidin-like domain may be novel targets for anti-myofibroblast-based therapies for the treatment of pulmonary fibrosis... - Identification of a CArG-independent region of the cysteine-rich protein 2 promoter that directs expression in the developing vasculatureYung Fu Chang
Pulmonary and Critical Care Division, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, 75 Francis St, Boston, MA 02115, USA
Am J Physiol Heart Circ Physiol 285:H1675-83. 2003.... - Cyclooxygenase-2-deficient mice are resistant to endotoxin-induced inflammation and deathKuniaki Ejima
Brigham and Women s Hospital, Pulmonary and Critical Care Medicine, 75 Francis St, Boston, MA 02115, USA
FASEB J 17:1325-7. 2003..These beneficial effects occur, in part, by a compensatory increase in IL-10 that counterbalances the pro-inflammatory host response to endotoxemia... - Modulation of the thioredoxin system during inflammatory responses and its effect on heme oxygenase-1 expressionKuniaki Ejima
Pulmonary and Critical Care, Brigham and Women s Hospital, Boston, MA 02115, USA
Antioxid Redox Signal 4:569-75. 2002..Taken together, these data support the concept that the TRX system contributes to the up-regulation of HO-1 under conditions associated with increased oxidative stress... - Heme oxygenase 1 in regulation of inflammation and oxidative damageShaw-Fang Yet
Pulmonary and Critical Care Division, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA
Methods Enzymol 353:163-76. 2002 - IFN regulatory factor-1 regulates IFN-gamma-dependent cathepsin S expressionKarin Storm van's Gravesande
Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02115, USA
J Immunol 168:4488-94. 2002..Thus, IRF-1 is the critical transcriptional mediator of IFN-gamma-dependent cathepsin S activation. These data elucidate a new pathway by which IRF-1 may affect MHC class II processing and presentation... - Gene therapy strategy for long-term myocardial protection using adeno-associated virus-mediated delivery of heme oxygenase geneLuis G Melo
Department of Medicine, Brigham and Women's Hospital, and Harvard Medical School, Boston, Massachusetts, USA
Circulation 105:602-7. 2002.... - Disruption of striated preferentially expressed gene locus leads to dilated cardiomyopathy in miceXiaoli Liu
Pulmonary and Critical Care Medicine, Brigham and Women s Hospital, and Harvard Medical School, Boston, MA 02115, USA
Circulation 119:261-8. 2009..Depending on the cell type, Speg isoforms may serve as markers of striated or smooth muscle differentiation... - Elk-3 is a transcriptional repressor of nitric-oxide synthase 2Yen Hsu Chen
Pulmonary and Critical Care Division, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 278:39572-7. 2003..Thus, Elk-3 may serve as an important mediator of NOS2 gene expression... - Increased neointima formation in cysteine-rich protein 2-deficient mice in response to vascular injuryJiao Wei
Pulmonary and Critical Care Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
Circ Res 97:1323-31. 2005..Our data demonstrate that CRP2 is not required for vascular development. However, an absence of CRP2 enhanced VSMC migration and increased neointima formation following arterial injury... - Distamycin A inhibits HMGA1-binding to the P-selectin promoter and attenuates lung and liver inflammation during murine endotoxemiaRebecca M Baron
Division of Pulmonary and Critical Care Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS ONE 5:e10656. 2010.... - Netropsin improves survival from endotoxaemia by disrupting HMGA1 binding to the NOS2 promoterMarianne A Grant
Division of Molecular and Vascular Medicine, Center for Vascular Biology Research, Department of Medicine, Beth Israel Deaconess Medical Center, 99 Brookline Avenue, Boston, MA 02215, USA
Biochem J 418:103-12. 2009.... - Aortic carboxypeptidase-like protein is regulated by transforming growth factor beta in 3T3-L1 preadipocytesAnneMarie Gagnon
Department of Medicine and Biochemistry, Ottawa Health Research Institute, 725 Parkdale Avenue, Ottawa, Ontario, Canada K1Y 4E9
Exp Cell Res 308:265-72. 2005..However, sustained expression of ACLP in stable clones of 3T3-L1 or 3T3-F442A preadipocytes did not interfere with adipogenesis... - Down-regulation of aortic carboxypeptidase-like protein during the early phase of 3T3-L1 adipogenesisAnneMarie Gagnon
Department of Medicine, Ottawa Health Research Institute, University of Ottawa, Canada K1Y 4E9
Endocrinology 143:2478-85. 2002..The role of ACLP and its marked reduction during adipogenesis merit further study... - Telomerase, myofibroblasts, and pulmonary fibrosisSCOTT L SCHISSEL
Am J Respir Cell Mol Biol 34:520-2. 2006