Research Topics
| Michael T LaubSummaryAffiliation: Harvard University Country: USA Publications
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Detail Information
Publications
Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysisJeffrey M Skerker
Bauer Center for Genomics Research, Harvard University, Cambridge, Massachusetts, USA
PLoS Biol 3:e334. 2005..We propose that this system-wide selectivity insulates two-component pathways from one another, preventing unwanted cross-talk...
Regulation of the bacterial cell cycle by an integrated genetic circuitEmanuele G Biondi
FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 444:899-904. 2006..Our results define a single integrated circuit whose components and connectivity can account for the cell cycle oscillations of CtrA in Caulobacter...- Dynamics of two Phosphorelays controlling cell cycle progression in Caulobacter crescentusY Erin Chen
Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Bacteriol 191:7417-29. 2009..More generally, our results emphasize how the bifunctional nature of histidine kinases can help switch cells between mutually exclusive states... - Systematic dissection and trajectory-scanning mutagenesis of the molecular interface that ensures specificity of two-component signaling pathwaysEmily J Capra
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
PLoS Genet 6:e1001220. 2010..Only some trajectories allow both the maintenance of phosphotransfer and the avoidance of unwanted cross-talk... - A dynamic complex of signaling proteins uses polar localization to regulate cell-fate asymmetry in Caulobacter crescentusChristos G Tsokos
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Dev Cell 20:329-41. 2011..Our results reveal the mechanisms by which CckA is regulated in a cell-type-dependent manner. More generally, our findings reveal how cells exploit subcellular localization to orchestrate sophisticated regulatory processes... - A phosphorelay system controls stalk biogenesis during cell cycle progression in Caulobacter crescentusEmanuele G Biondi
Bauer Center for Genomics Research, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
Mol Microbiol 59:386-401. 2006..This is the first example of a general method for identifying the connectivity of a phosphorelay and can be applied to any organism with two-component signal transduction systems... - Rewiring the specificity of two-component signal transduction systemsJeffrey M Skerker
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Cell 133:1043-54. 2008.... - Modularity of the bacterial cell cycle enables independent spatial and temporal control of DNA replicationKristina Jonas
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Curr Biol 21:1092-101. 2011..Complex regulatory circuits in biology are often built of simpler subcircuits or modules. In most cases, the functional consequences and evolutionary origins of modularity remain poorly defined... - Spatial gradient of protein phosphorylation underlies replicative asymmetry in a bacteriumY Erin Chen
Department of Biology and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 108:1052-7. 2011..More generally, our results demonstrate that uniform protein abundance may belie gradients and other sophisticated spatial patterns of protein activity in bacterial cells... - A cell-type-specific protein-protein interaction modulates transcriptional activity of a master regulator in Caulobacter crescentusKasia G Gora
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Mol Cell 39:455-67. 2010..CtrA is thus tightly regulated by a protein-protein interaction which is critical to cell-cycle progression... - Determinants of homodimerization specificity in histidine kinasesOrr Ashenberg
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Mol Biol 413:222-35. 2011..EnvZ and RstB likely diverged following gene duplication to yield two homodimers that cannot heterodimerize, and the mutants we identified represent possible evolutionary intermediates in this process... - Phosphotransfer profiling: systematic mapping of two-component signal transduction pathways and phosphorelaysMichael T Laub
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
Methods Enzymol 423:531-48. 2007..The technique can be further extended to mapping phosphorelays and the cognate partners of histidine phosphotransferases. Here, we describe protocols and strategies for the successful implementation of this system-level technique... - Direct and adaptor-mediated substrate recognition by an essential AAA+ proteasePeter Chien
Department of Biology and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 104:6590-5. 2007.... - Specificity in two-component signal transduction pathwaysMichael T Laub
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Annu Rev Genet 41:121-45. 2007.... - A DNA damage checkpoint in Caulobacter crescentus inhibits cell division through a direct interaction with FtsWJoshua W Modell
Department of Biology, Massachusetts Institute of Technology, Cambridge, USA
Genes Dev 25:1328-43. 2011..Instead, we provide evidence that SidA inhibits division by binding directly to FtsW to prevent the final constriction of the cytokinetic ring... - Cell-cycle progression and the generation of asymmetry in Caulobacter crescentusJeffrey M Skerker
Bauer Center for Genomics Research, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA
Nat Rev Microbiol 2:325-37. 2004 - Systems biology of CaulobacterMichael T Laub
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Annu Rev Genet 41:429-41. 2007....