Ashish Lal

Summary

Affiliation: Harvard Medical School
Country: USA

Publications

  1. pmc p16(INK4a) translation suppressed by miR-24
    Ashish Lal
    Laboratory of Cellular and Molecular Biology, National Institute on Aging IRP, National Institutes of Health, Baltimore, Maryland, United States of America
    PLoS ONE 3:e1864. 2008
  2. pmc Ubiquitin-mediated proteolysis of HuR by heat shock
    Kotb Abdelmohsen
    Laboratory of Cellular and Molecular Biology, NIA IRP, NIH, Baltimore, MD 21224, USA
    EMBO J 28:1271-82. 2009
  3. pmc Translational control of cytochrome c by RNA-binding proteins TIA-1 and HuR
    Tomoko Kawai
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Intramural Research Program NIH, Box 12, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Mol Cell Biol 26:3295-307. 2006
  4. ncbi Posttranscriptional orchestration of an anti-apoptotic program by HuR
    Kotb Abdelmohsen
    Laboratory of Cellular and Molecular Biology, National Institute on Aging IRP, National Institutes of Health, Baltimore, Maryland 21224, USA
    Cell Cycle 6:1288-92. 2007
  5. pmc Nuclear HuR accumulation through phosphorylation by Cdk1
    Hyeon Ho Kim
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    Genes Dev 22:1804-15. 2008
  6. pmc Analysis of turnover and translation regulatory RNA-binding protein expression through binding to cognate mRNAs
    Rudolf Pullmann
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21228, USA
    Mol Cell Biol 27:6265-78. 2007
  7. pmc Phosphorylation of HuR by Chk2 regulates SIRT1 expression
    Kotb Abdelmohsen
    Laboratory of Cellular and Molecular Biology, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Mol Cell 25:543-57. 2007
  8. ncbi Posttranscriptional derepression of GADD45alpha by genotoxic stress
    Ashish Lal
    Laboratory of Cellular and Molecular Biology, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Mol Cell 22:117-28. 2006
  9. pmc Growth inhibition by miR-519 via multiple p21-inducing pathways
    Kotb Abdelmohsen
    Laboratory of Molecular Biology and Immunology, Research Resources Branch, National Institute on Aging, NIH, Baltimore, Maryland, USA
    Mol Cell Biol 32:2530-48. 2012
  10. ncbi Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1
    Rudolf Pullmann
    Laboratory of Cellular and Molecular Biology, NIA IRP, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 281:23456-63. 2006

Collaborators

Detail Information

Publications21

  1. pmc p16(INK4a) translation suppressed by miR-24
    Ashish Lal
    Laboratory of Cellular and Molecular Biology, National Institute on Aging IRP, National Institutes of Health, Baltimore, Maryland, United States of America
    PLoS ONE 3:e1864. 2008
    ..Expression of the tumor suppressor p16(INK4a) increases during aging and replicative senescence...
  2. pmc Ubiquitin-mediated proteolysis of HuR by heat shock
    Kotb Abdelmohsen
    Laboratory of Cellular and Molecular Biology, NIA IRP, NIH, Baltimore, MD 21224, USA
    EMBO J 28:1271-82. 2009
    ..Our findings reveal that HS transiently lowers HuR by proteolysis linked to K182 ubiquitination and that HuR reduction enhances cell survival following HS...
  3. pmc Translational control of cytochrome c by RNA-binding proteins TIA-1 and HuR
    Tomoko Kawai
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Intramural Research Program NIH, Box 12, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    Mol Cell Biol 26:3295-307. 2006
    ....
  4. ncbi Posttranscriptional orchestration of an anti-apoptotic program by HuR
    Kotb Abdelmohsen
    Laboratory of Cellular and Molecular Biology, National Institute on Aging IRP, National Institutes of Health, Baltimore, Maryland 21224, USA
    Cell Cycle 6:1288-92. 2007
    ....
  5. pmc Nuclear HuR accumulation through phosphorylation by Cdk1
    Hyeon Ho Kim
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    Genes Dev 22:1804-15. 2008
    ..Our findings suggest that Cdk1 phosphorylates HuR during G2, thereby helping to retain it in the nucleus in association with 14-3-3 and hindering its post-transcriptional function and anti-apoptotic influence...
  6. pmc Analysis of turnover and translation regulatory RNA-binding protein expression through binding to cognate mRNAs
    Rudolf Pullmann
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21228, USA
    Mol Cell Biol 27:6265-78. 2007
    ..Together, our findings underscore the notion that TTR-RBP expression is controlled, at least in part, at the posttranscriptional level through a complex circuitry of self- and cross-regulatory RNP interactions...
  7. pmc Phosphorylation of HuR by Chk2 regulates SIRT1 expression
    Kotb Abdelmohsen
    Laboratory of Cellular and Molecular Biology, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA
    Mol Cell 25:543-57. 2007
    ..Our findings demonstrate that HuR regulates SIRT1 expression, underscore functional links between the two stress-response proteins, and implicate Chk2 in these processes...
  8. ncbi Posttranscriptional derepression of GADD45alpha by genotoxic stress
    Ashish Lal
    Laboratory of Cellular and Molecular Biology, Intramural Research Program, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
    Mol Cell 22:117-28. 2006
    ..We propose that the posttranscriptional derepression of GADD45alpha critically contributes to its potent upregulation after DNA damage...
  9. pmc Growth inhibition by miR-519 via multiple p21-inducing pathways
    Kotb Abdelmohsen
    Laboratory of Molecular Biology and Immunology, Research Resources Branch, National Institute on Aging, NIH, Baltimore, Maryland, USA
    Mol Cell Biol 32:2530-48. 2012
    ..Our results indicate that miR-519 promotes DNA damage, alters Ca(2+) homeostasis, and enhances energy production; together, these processes elevate the expression level of p21, promoting growth inhibition and cell survival...
  10. ncbi Differential stability of thymidylate synthase 3'-untranslated region polymorphic variants regulated by AUF1
    Rudolf Pullmann
    Laboratory of Cellular and Molecular Biology, NIA IRP, National Institutes of Health, Baltimore, Maryland 21224, USA
    J Biol Chem 281:23456-63. 2006
    ..Our results illustrate the functional consequences of ribonucleoprotein associations involving a polymorphic RNA sequence and uncover a novel mechanism of action for non-coding RNA polymorphisms...
  11. pmc Translational repression by RNA-binding protein TIAR
    Krystyna Mazan-Mamczarz
    Laboratory of Cellular and Molecular Biology, Box 12, LCMB, National Institute on Aging Intramural Research Program, National Institutes of Health, 5600 Nathan Shock Dr, Baltimore, Maryland 21224, USA
    Mol Cell Biol 26:2716-27. 2006
    ..We propose that the TIAR-mediated inhibition of translation factor expression elicits a sustained repression of protein biosynthesis in cells responding to stress...
  12. pmc Antiapoptotic function of RNA-binding protein HuR effected through prothymosin alpha
    Ashish Lal
    Laboratory of Cellular and Molecular Biology, National Institute on Aging IRP, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224, USA
    EMBO J 24:1852-62. 2005
    ..Together, our data support a regulatory scheme whereby HuR binds the ProTalpha mRNA, elevates its cytoplasmic abundance and translation, and thereby elicits an antiapoptotic program...
  13. ncbi HuR: post-transcriptional paths to malignancy
    Isabel Lopez de Silanes
    Laboratory of Cellular and Molecular Biology, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA
    RNA Biol 2:11-3. 2005
    ..We describe a paradigm consistent with a central role of HuR in oncogenesis...
  14. pmc Identification of a signature motif in target mRNAs of RNA-binding protein AUF1
    Krystyna Mazan-Mamczarz
    Laboratory of Cellular and Molecular Biology, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD 21224, USA
    Nucleic Acids Res 37:204-14. 2009
    ....
  15. ncbi Prostaglandin A2-mediated stabilization of p21 mRNA through an ERK-dependent pathway requiring the RNA-binding protein HuR
    Xiaoling Yang
    Laboratory of Cellular and Molecular Biology, NIA, National Institutes of Health, Baltimore, MD 21224, USA
    J Biol Chem 279:49298-306. 2004
    ..Collectively, our results indicate that PGA2 stabilizes the p21 mRNA through an ERK-independent increase in cytoplasmic HuR levels and an ERK-dependent association of HuR with the p21 mRNA...
  16. pmc A p21-ZEB1 complex inhibits epithelial-mesenchymal transition through the microRNA 183-96-182 cluster
    Xiao Ling Li
    Genetics Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA
    Mol Cell Biol 34:533-50. 2014
    ..Furthermore, we found that p21 forms a complex with ZEB1 at the miR-183-96-182 cluster promoter to inhibit transcriptional repression of this cluster by ZEB1, suggesting a reciprocal feedback loop. ..
  17. ncbi Egad, more forms of gene regulation: the gadd45a story
    Ashish Lal
    Laboratory of Cellular and Molecular Biology, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224, USA
    Cell Cycle 5:1422-5. 2006
    ....
  18. ncbi Evidence for miR-181 involvement in neuroinflammatory responses of astrocytes
    Emmette R Hutchison
    Laboratory of Neurosciences, National Institute on Aging, NIH, Baltimore, Maryland, 21224, USA
    Glia 61:1018-28. 2013
    ..Further understanding of the role of miR-181 in inflammatory events and CNS injury could lead to novel approaches for the treatment of CNS disorders with an inflammatory component...
  19. pmc Clean Western blot signals from immunoprecipitated samples
    Ashish Lal
    Laboratory of Cellular and Molecular Biology, National Institute on Aging IRP, National Institutes of Health, Box 12, 5600 Nathan Shock Dr, Baltimore, MD 21224, USA
    Mol Cell Probes 19:385-8. 2005
    ..Here, we show that HRP-conjugated Protein A and Protein G, which detect almost exclusively intact antibody molecules, can be effectively used to obtain clean and specific WB signals of target proteins...
  20. pmc Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs
    Ashish Lal
    Laboratory of Cellular and Molecular Biology, National Institute on Aging IRP, National Institutes of Health, Baltimore, MD 21224, USA
    EMBO J 23:3092-102. 2004
    ....
  21. pmc RNA-binding proteins HuR and PTB promote the translation of hypoxia-inducible factor 1alpha
    Stefanie Galban
    LCMB, NIA, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224, USA
    Mol Cell Biol 28:93-107. 2008
    ..Conversely, HIF-1alpha expression and translation in response to CoCl(2) were markedly elevated after HuR overexpression. We propose that HuR and PTB jointly upregulate HIF-1alpha translation in response to CoCl(2)...