Research Topics
| Anand T N KumarSummaryAffiliation: Harvard University Country: USA Publications
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Detail Information
Publications
Direct Monte Carlo computation of time-resolved fluorescence in heterogeneous turbid mediaAnand T N Kumar
Athinoula A Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
Opt Lett 37:4783-5. 2012..We validate this method using simulations with both a slab and a heterogeneous model of the mouse head...
Fluorescence lifetime-based optical molecular imagingAnand T N Kumar
Athinoula A Martinos Center for Biomedical Imaging, Harvard Medical School, Massachusetts General Hospital, Charlestown, MA 02129, USA
Methods Mol Biol 680:165-80. 2011..This feature has potential applications for longitudinal pre-clinical evaluation of drug treatment response as well as to address fundamental questions related to tumor physiology and metastasis...
Feasibility of in vivo imaging of fluorescent proteins using lifetime contrastAnand T N Kumar
Athinoula A Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
Opt Lett 34:2066-8. 2009..These results suggest the potential for exploiting fluorescence lifetime for imaging FPs for a variety of whole-body small-animal imaging applications...
A time domain fluorescence tomography system for small animal imagingAnand T N Kumar
Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA
IEEE Trans Med Imaging 27:1152-63. 2008..These results suggest the practical feasibility and advantages of a time domain approach for whole body small animal fluorescence molecular imaging, particularly with the use of lifetime as a contrast mechanism...
Comparison of frequency-domain and time-domain fluorescence lifetime tomographyAnand T N Kumar
Athinoula A Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
Opt Lett 33:470-2. 2008..We also demonstrate experimentally, using complex-shaped phantoms, the advantages of the asymptotic time-domain approach over a Fourier-based approach for analyzing time-domain fluorescence data...
Fluorescence-lifetime-based tomography for turbid mediaAnand T N Kumar
Athinoula A Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
Opt Lett 30:3347-9. 2005....
Two postprocessing techniques for the elimination of background autofluorescence for fluorescence lifetime imaging microscopyPhillip B Jones
Harvard Medical School, Massachusetts General Hospital, MassGeneral Institute for Neurodegenerative Disorders, 114 16th Street, Charlestown, Massachusetts 02129, USA
J Biomed Opt 13:014008. 2008....
Lifetime-based tomographic multiplexingScott B Raymond
The Harvard MIT Division of Health Sciences and Technology, Charlestown, Massachusetts 02129, USA
J Biomed Opt 15:046011. 2010..Finally, we demonstrate tomographic imaging of two and three fluorophores in nude mice with fluorophores that localize to distinct organ systems. This technique should be widely applicable to imaging multiple NIR fluorophores in 3-D...
Time-domain fluorescent plate reader for cell based protein-protein interaction and protein conformation assaysPhill B Jones
Athinoula A. Martinos Center for Biomedical Imaging, Harvard Medical School, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA
J Biomed Opt 11:054024. 2006..Presenilin 1 (PS1) is known to be important in Amyloid Precursor Protein (APP) processing in Alzheimer's disease. Using transfected cells, we demonstrate APP-PS1 interactions by FRET in a cell-based, 96-well plate format...
Sensing of transcription factor binding via cyanine dye pair fluorescence lifetime changesAlexei A Bogdanov
The Laboratory of Molecular Imaging Probes S6 434, Department of Radiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA
Mol Biosyst 8:2166-73. 2012..We anticipate that the observed effects will be instrumental for developing sensors enabling non-invasive imaging in cells that undergo activation of NF-κB...
The PEG-fluorochrome shielding approach for targeted probe designYanyan Guo
Center for Translational Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital, 149 13th Street, Charlestown, Massachusetts 02129, USA
J Am Chem Soc 134:19338-41. 2012..Using PEG to block fluorochrome-mediated interactions, rather than synthesizing de novo fluorochromes, can yield new approaches for the design of actively or passively targeted near-infrared fluorescent probes...
