- Thin PDMS films using long spin times or tert-butyl alcohol as a solventJohn H Koschwanez
Department of Electrical Engineering, University of Washington, Seattle, Washington, United States of America
PLoS ONE 4:e4572. 2009..The use of tert-butyl alcohol to spin thin PDMS films does not swell the underlying PDMS substrate, and we have used these films to construct multilayer PDMS devices...
- Improved use of a public good selects for the evolution of undifferentiated multicellularityJohn H Koschwanez
FAS Center for Systems Biology and Department of Molecular and Cellular Biology, Harvard University, Cambridge, United States
elife 2:e00367. 2013..Our study shows that combining rational design with experimental evolution can help evaluate hypotheses about evolutionary strategies. DOI:http://dx.doi.org/10.7554/eLife.00367.001...
- Sucrose utilization in budding yeast as a model for the origin of undifferentiated multicellularityJohn H Koschwanez
FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts, United States of America
PLoS Biol 9:e1001122. 2011..We propose that the prior use of public goods led to selection for the incomplete cell separation that first produced multicellularity...
- Easily fabricated magnetic traps for single-cell applicationsJohn H Koschwanez
Department of Electrical Engineering, University of Washington, Seattle, WA 98195 2500, USA
Rev Sci Instrum 78:044301. 2007..We demonstrate individual yeast cell capture and estimate the capture force (1.9-4.4 pN) by measuring the flow speed required to remove the cell from its trap in a microfluidic channel...