NANCY E KLECKNER

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. doi request reprint Crossover patterning by the beam-film model: analysis and implications
    Liangran Zhang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS Genet 10:e1004042. 2014
  2. pmc Dynamic trans interactions in yeast chromosomes
    Ekaterina V Mirkin
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 8:e75895. 2013
  3. ncbi request reprint Chiasma formation: chromatin/axis interplay and the role(s) of the synaptonemal complex
    Nancy Kleckner
    Harvard University, Cambridge, MA 02138, USA
    Chromosoma 115:175-94. 2006
  4. pmc A mechanical basis for chromosome function
    Nancy Kleckner
    Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 101:12592-7. 2004
  5. ncbi request reprint Coordinate variation in meiotic pachytene SC length and total crossover/chiasma frequency under conditions of constant DNA length
    Nancy Kleckner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Trends Genet 19:623-8. 2003
  6. ncbi request reprint ATR homolog Mec1 promotes fork progression, thus averting breaks in replication slow zones
    Rita S Cha
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 297:602-6. 2002
  7. ncbi request reprint Capturing chromosome conformation
    Job Dekker
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:1306-11. 2002
  8. pmc Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation
    David Bates
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 121:899-911. 2005
  9. pmc The Escherichia coli baby cell column: a novel cell synchronization method provides new insight into the bacterial cell cycle
    David Bates
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Microbiol 57:380-91. 2005
  10. doi request reprint Assaying chromosome pairing by FISH analysis of spread Saccharomyces cerevisiae nuclei
    Beth M Weiner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA
    Methods Mol Biol 558:37-51. 2009

Collaborators

Detail Information

Publications19

  1. doi request reprint Crossover patterning by the beam-film model: analysis and implications
    Liangran Zhang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS Genet 10:e1004042. 2014
    ..Relationships to other models are discussed. ..
  2. pmc Dynamic trans interactions in yeast chromosomes
    Ekaterina V Mirkin
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 8:e75895. 2013
    ..The three-dimensional organization of the yeast nucleus imposes a constraint on such pairing, presumably by dictating the probability with which the two sequences collide with one another. ..
  3. ncbi request reprint Chiasma formation: chromatin/axis interplay and the role(s) of the synaptonemal complex
    Nancy Kleckner
    Harvard University, Cambridge, MA 02138, USA
    Chromosoma 115:175-94. 2006
    ..Furthermore, we discuss implications and extensions of our recently proposed mechanical model for chiasma formation. Finally, we give a broader consideration to past and present models for the role of the synaptonemal complex...
  4. pmc A mechanical basis for chromosome function
    Nancy Kleckner
    Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 101:12592-7. 2004
    ..We propose that chromosome function is governed by internal mechanical forces generated by programmed tendencies for expansion of the DNA/chromatin fiber against constraining features...
  5. ncbi request reprint Coordinate variation in meiotic pachytene SC length and total crossover/chiasma frequency under conditions of constant DNA length
    Nancy Kleckner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Trends Genet 19:623-8. 2003
  6. ncbi request reprint ATR homolog Mec1 promotes fork progression, thus averting breaks in replication slow zones
    Rita S Cha
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 297:602-6. 2002
    ..Thus, Mec1 has important functions in normal S phase and the genome instability of mec1 (and, analogously, ATR-/-) mutants stems from defects in these basic roles...
  7. ncbi request reprint Capturing chromosome conformation
    Job Dekker
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Science 295:1306-11. 2002
    ..Furthermore, functionally distinct AT- and GC-rich domains were found to exhibit different conformations, and a population-average 3D model of chromosome III could be determined. Chromosome III emerges as a contorted ring...
  8. pmc Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation
    David Bates
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cell 121:899-911. 2005
    ..We propose that cell division licenses the next round of replication initiation via these changes...
  9. pmc The Escherichia coli baby cell column: a novel cell synchronization method provides new insight into the bacterial cell cycle
    David Bates
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Microbiol 57:380-91. 2005
    ..Second, at a conceptual level, replication-related events are more tightly linked to cell birth (i.e. cell division) than to absolute cell mass...
  10. doi request reprint Assaying chromosome pairing by FISH analysis of spread Saccharomyces cerevisiae nuclei
    Beth M Weiner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA
    Methods Mol Biol 558:37-51. 2009
    ..Described here is a method for FISH analysis and its application to analysis of chromosome pairing during meiosis in S. cerevisiae...
  11. pmc Yeast Pch2 promotes domainal axis organization, timely recombination progression, and arrest of defective recombinosomes during meiosis
    G Valentin Börner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 105:3327-32. 2008
    ..We further propose that regulatory surveillance of normal and abnormal interchromosomal interactions in mitotic and meiotic cells may involve "structure-dependent interchromosomal interaction" (SDIX) checkpoints...
  12. ncbi request reprint Physical and functional interactions among basic chromosome organizational features govern early steps of meiotic chiasma formation
    Yuval Blat
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 111:791-802. 2002
    ..These complexities are discussed in the context of chiasma formation as a series of coordinated local changes at the DNA and chromosome-axis levels...
  13. ncbi request reprint Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis
    G Valentin Börner
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Cell 117:29-45. 2004
    ....
  14. pmc Bioinformatic analyses implicate the collaborating meiotic crossover/chiasma proteins Zip2, Zip3, and Spo22/Zip4 in ubiquitin labeling
    Jason Perry
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 102:17594-9. 2005
    ..These and other findings suggest that Zip2, Zip3, and Zip4 act together to mediate a process that involves Zip3-mediated ubiquitin labeling, potentially as a unique type of ubiquitin-conjugating complex...
  15. pmc Meiotic double-strand breaks occur once per pair of (sister) chromatids and, via Mec1/ATR and Tel1/ATM, once per quartet of chromatids
    Liangran Zhang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 108:20036-41. 2011
    ..The two types of constraints on DSB formation provide two different safeguards against recombination failure during meiosis...
  16. pmc PR65, the HEAT-repeat scaffold of phosphatase PP2A, is an elastic connector that links force and catalysis
    Alison Grinthal
    Departments of Molecular and Cellular Biology, and Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 107:2467-72. 2010
    ..Given that helical-repeat proteins are involved in many cellular processes, the findings also encourage the view that mechanical forces may be of widespread importance...
  17. pmc Examination of interchromosomal interactions in vegetatively growing diploid Schizosaccharomyces pombe cells by Cre/loxP site-specific recombination
    Monika Molnar
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genetics 178:99-112. 2008
    ..We discuss the factors whose interplay sets the probabilities of chromosomal interactions in this organism and implications of the inferred organization for ectopic recombination...
  18. pmc Tsr-GFP accumulates linearly with time at cell poles, and can be used to differentiate 'old' versus 'new' poles, in Escherichia coli
    Liyan Ping
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Microbiol 69:1427-38. 2008
    ..The absolute level of Tsr-GFP at the old pole of a cell also provides a rough estimate of pole (and thus cell) age...
  19. pmc The structure of DNA overstretched from the 5'5' ends differs from the structure of DNA overstretched from the 3'3' ends
    Claudia Danilowicz
    Department of Physics, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 106:13196-201. 2009
    ..These differences and additional findings are well-accommodated by the corresponding structures predicted on theoretical grounds...

Research Grants43

  1. ILLEGITIMATE RECOMBINATION BY DRUG RESISTANCE ELEMENTS
    NANCY E KLECKNER; Fiscal Year: 2010
    ..Since strategies for public health depend upon, and are nucleated by, an understanding of basic processes, research from this relatively new perspective is likely to lead ultimately to new approaches and thus to improved human health. ..
  2. ILLEGITIMATE RECOMBINATION BY DRUG RESISTANCE ELEMENTS
    Nancy Kleckner; Fiscal Year: 2004
    ..We will begin to investigate the hypothesis that cell cycle progression is governed by SLS's (sensors of local stress) and that Mec1/Esr1 is an integral component of this machinery and is a stress-dependent kinase. ..
  3. MEIOTIC CHROMOSOME SYNAPSIS AND RECOMBINATION IN YEAST
    Nancy Kleckner; Fiscal Year: 2002
    ..We will further investigate chromosomal protein Bdf1 and variations in histone status in this regard by genetic, C-SCAN and other methods. Supercoiling status of loops may be addressed. ..
  4. MEIOTIC CHROMOSOME SYNAPSIS AND RECOMBINATION IN YEAST
    NANCY E KLECKNER; Fiscal Year: 2010
    ..g. Downs syndrome). Basic understanding of these processes is crucial for diagnosis and potential amelioration of these conditions. ..
  5. MEIOTIC CHROMOSOME SYNAPSIS AND RECOMBINATION IN YEAST
    NANCY E KLECKNER; Fiscal Year: 2010
    ..g. Downs syndrome). Basic understanding of these processes is crucial for diagnosis and potential amelioration of these conditions. ..
  6. MEIOTIC CHROMOSOME SYNAPSIS AND RECOMBINATION IN YEAST
    Nancy Kleckner; Fiscal Year: 2009
    ..g. Downs syndrome). Basic understanding of these processes is crucial for diagnosis and potential amelioration of these conditions. ..
  7. MEIOTIC CHROMOSOME SYNAPSIS AND RECOMBINATION IN YEAST
    Nancy Kleckner; Fiscal Year: 2007
    ..g. Downs syndrome). Basic understanding of these processes is crucial for diagnosis and potential amelioration of these conditions. ..
  8. ILLEGITIMATE RECOMBINATION BY DRUG RESISTANCE ELEMENTS
    Nancy Kleckner; Fiscal Year: 2007
    ....
  9. Meiotic chromosome synapsis and recombination in yeast.
    Nancy Kleckner; Fiscal Year: 2006
    ..We will examine the dynamics of synaptonemal complex twisting in vivo. We will begin to develop methods for isolating, analyzing and physically manipulating pachytene chromosomes in vitro. ..
  10. ILLEGITIMATE RECOMBINATION BY DRUG RESISTANCE ELEMENTS
    Nancy Kleckner; Fiscal Year: 1990
    ..7) Regulation of IS10 transposition by dam methylation: kinetics of methylation on non-replicating and replicating DNA in vivo and in vitro...
  11. ILLEGITIMATE RECOMBINATION BY DRUG RESISTANCE ELEMENTS
    Nancy Kleckner; Fiscal Year: 1980
    ..We hope that the results will give us new insight into the ways in which known DNA sites and segments interact during Tn10-promoted recombination events...
  12. MEIOTIC CHROMOSOME SYNAPSIS AND RECOMBINATION IN YEAST
    Nancy Kleckner; Fiscal Year: 1993
    ..Genes for major structural proteins, i.e. SC components, should be preferentially recovered; new meiosis-specific structures could also be identified...