TOMAS L KIRCHHAUSEN

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Cholera toxin toxicity does not require functional Arf6- and dynamin-dependent endocytic pathways
    Ramiro H Massol
    Department of Cell Biology, Harvard Medical School and The Center for Blood Research for Biomedical Research, Boston, Massachusetts 02115, USA
    Mol Biol Cell 15:3631-41. 2004
  2. doi request reprint Bending membranes
    Tom Kirchhausen
    Department of Cell Biology, Harvard Medical School and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Cell Biol 14:906-8. 2012
  3. pmc Limited transferrin receptor clustering allows rapid diffusion of canine parvovirus into clathrin endocytic structures
    David K Cureton
    Immune Disease Institute and Program in Cellular and Molecular Medicine at Children s Hospital Boston, Boston, Massachusetts, USA
    J Virol 86:5330-40. 2012
  4. pmc Imaging endocytic clathrin structures in living cells
    Tom Kirchhausen
    Harvard Medical School Immune Disease Institute, W Alpert Building Room 128, Boston, MA 02115, USA
    Trends Cell Biol 19:596-605. 2009
  5. ncbi request reprint Making COPII coats
    Tomas Kirchhausen
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115 5701, USA
    Cell 129:1251-2. 2007
  6. ncbi request reprint Wiskott-Aldrich syndrome: a gene, a multifunctional protein and the beginnings of an explanation
    T Kirchhausen
    Center for Blood Research, Boston, MA 02115, USA
    Mol Med Today 4:300-4. 1998
  7. ncbi request reprint Clathrin
    T Kirchhausen
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Biochem 69:699-727. 2000
  8. ncbi request reprint Adaptors for clathrin-mediated traffic
    T Kirchhausen
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Cell Dev Biol 15:705-32. 1999
  9. ncbi request reprint Three ways to make a vesicle
    T Kirchhausen
    Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 1:187-98. 2000
  10. ncbi request reprint Vesicle formation: dynamic dynamin lives up to its name
    T Kirchhausen
    Department of Cell Biology and Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Biol 8:R792-4. 1998

Research Grants

  1. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2010
  2. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2006
  3. Dynamics of Clathrin Coat Formation in Cells
    Tomas Kirchhausen; Fiscal Year: 2007
  4. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2007
  5. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2002
  6. STRUCTURAL CHEMISTRY OF CLATHRIN & ASSOCIATED PROTEINS
    Tomas Kirchhausen; Fiscal Year: 1993

Collaborators

Detail Information

Publications24

  1. pmc Cholera toxin toxicity does not require functional Arf6- and dynamin-dependent endocytic pathways
    Ramiro H Massol
    Department of Cell Biology, Harvard Medical School and The Center for Blood Research for Biomedical Research, Boston, Massachusetts 02115, USA
    Mol Biol Cell 15:3631-41. 2004
    ..These results are consistent with the existence of an additional retrograde pathway used by CT to reach the ER...
  2. doi request reprint Bending membranes
    Tom Kirchhausen
    Department of Cell Biology, Harvard Medical School and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Cell Biol 14:906-8. 2012
    ..The second reports that membrane crowding, driven by protein-protein interactions, can also drive membrane bending, even in the absence of any protein insertion into the bilayer...
  3. pmc Limited transferrin receptor clustering allows rapid diffusion of canine parvovirus into clathrin endocytic structures
    David K Cureton
    Immune Disease Institute and Program in Cellular and Molecular Medicine at Children s Hospital Boston, Boston, Massachusetts, USA
    J Virol 86:5330-40. 2012
    ....
  4. pmc Imaging endocytic clathrin structures in living cells
    Tom Kirchhausen
    Harvard Medical School Immune Disease Institute, W Alpert Building Room 128, Boston, MA 02115, USA
    Trends Cell Biol 19:596-605. 2009
    ..Here, I outline descriptions of coat assembly in different circumstances that are consistent with what is known from X-ray crystallography and electron microscopy...
  5. ncbi request reprint Making COPII coats
    Tomas Kirchhausen
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115 5701, USA
    Cell 129:1251-2. 2007
    ..Their findings suggest a mechanism by which the COPII cage assembles and accommodates cargo of different sizes...
  6. ncbi request reprint Wiskott-Aldrich syndrome: a gene, a multifunctional protein and the beginnings of an explanation
    T Kirchhausen
    Center for Blood Research, Boston, MA 02115, USA
    Mol Med Today 4:300-4. 1998
    ..Thus, WASP appears to integrate these two basic and fundamental cellular mechanisms. Several groups are now focusing on understanding the function of WASP in detail, and translating this new knowledge into improved therapies...
  7. ncbi request reprint Clathrin
    T Kirchhausen
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Biochem 69:699-727. 2000
    ..This review summarizes and illustrates the recent structural results and outlines what is known about coated-vesicle assembly in the context of this information...
  8. ncbi request reprint Adaptors for clathrin-mediated traffic
    T Kirchhausen
    Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Cell Dev Biol 15:705-32. 1999
    ..This review focuses on the most recent advances dealing with the molecular basis for sorting by clathrin adaptors...
  9. ncbi request reprint Three ways to make a vesicle
    T Kirchhausen
    Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Rev Mol Cell Biol 1:187-98. 2000
    ..A general model to explain the formation of coated vesicles is starting to emerge but the picture is more complex than we had imagined...
  10. ncbi request reprint Vesicle formation: dynamic dynamin lives up to its name
    T Kirchhausen
    Department of Cell Biology and Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Biol 8:R792-4. 1998
    ....
  11. pmc Exo1: a new chemical inhibitor of the exocytic pathway
    Yan Feng
    Institute of Chemistry and Cell Biology, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:6469-74. 2003
    ..Thus, Exo1 allows the fatty acid exchange activity of Bars50 to be distinguished from ARF1 activity in the control of Golgi tubulation...
  12. pmc Retrograde transport of cholera toxin from the plasma membrane to the endoplasmic reticulum requires the trans-Golgi network but not the Golgi apparatus in Exo2-treated cells
    Yan Feng
    Department of Cell Biology, Institute of Chemistry and Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
    EMBO Rep 5:596-601. 2004
    ..We propose that even in the absence of Exo2 the glycolipid pathway that carries the toxin from plasma membrane into the ER bypasses the Golgi apparatus entirely...
  13. pmc Chemical genetic screening identifies sulfonamides that raise organellar pH and interfere with membrane traffic
    Thomas J F Nieland
    Harvard Medical School, Department of Cell Biology and The CBR Institute for Biomedical Research, 200 Longwood Avenue, Boston MA, 02115, USA
    Traffic 5:478-92. 2004
    ..Identification of this novel class of sulfonamide inhibitors provides new chemical tools to better understand the function of organelle pH in membrane traffic and the activity of V-ATPases in particular...
  14. pmc Vesicular stomatitis virus enters cells through vesicles incompletely coated with clathrin that depend upon actin for internalization
    David K Cureton
    Departments of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 5:e1000394. 2009
    ..This work provides new mechanistic insights into the process of virus internalization as well as uptake of unconventional cargo by the clathrin-dependent endocytic machinery...
  15. pmc HIV Nef-mediated major histocompatibility complex class I down-modulation is independent of Arf6 activity
    Jakob E Larsen
    Department of Cell Biology and The Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 15:323-31. 2004
    ..We conclude that the apparent Arf6 dependency of Nef-mediated MHC class I down-modulation is due to nonspecific perturbations in membrane traffic...
  16. pmc Endosomal recycling controls plasma membrane area during mitosis
    Emmanuel Boucrot
    Department of Cell Biology and CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:7939-44. 2007
    ..We propose a mechanism by which modulation of endosomal recycling controls cell area and surface expression of membrane-bound proteins during cell division...
  17. pmc Distinct dynamics of endocytic clathrin-coated pits and coated plaques
    Saveez Saffarian
    Department of Cell Biology, Harvard Medical School, Children s Hospital and Immune Disease Institute, Boston, Massachusetts, USA
    PLoS Biol 7:e1000191. 2009
    ....
  18. pmc Discovery of chemical inhibitors of the selective transfer of lipids mediated by the HDL receptor SR-BI
    Thomas J F Nieland
    Department of Cell Biology, Harvard Medical School, and Center for Blood Research, Boston, MA 02115 5701, USA
    Proc Natl Acad Sci U S A 99:15422-7. 2002
    ....
  19. pmc Differential evanescence nanometry: live-cell fluorescence measurements with 10-nm axial resolution on the plasma membrane
    Saveez Saffarian
    Department of Cell Biology and Immune Disease Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biophys J 94:2333-42. 2008
    ..We find a gradually increasing axial separation between the centroids of clathrin and AP-2 distribution, up to a final value of 30 nm just before coated-pit pinching and formation of the coated vesicle...
  20. ncbi request reprint Targeting of AMSH to endosomes is required for epidermal growth factor receptor degradation
    Yu May Ma
    CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 282:9805-12. 2007
    ....
  21. pmc Association of Dishevelled with the clathrin AP-2 adaptor is required for Frizzled endocytosis and planar cell polarity signaling
    Anan Yu
    Department of Cell Biology and CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 12:129-41. 2007
    ..We suggest that the direct interaction of Dvl2 with AP-2 is important for Frizzled internalization and Frizzled/PCP signaling...
  22. ncbi request reprint Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitro
    Henry E Pelish
    Department of Cell Biology and The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Chem Biol 2:39-46. 2006
    ..RhoGDI-dependent Cdc42 inhibition by secramine illustrates a new way to inhibit Rho GTPases with small molecules and provides a new means to study Cdc42, RhoGDI and the cellular processes they mediate...
  23. ncbi request reprint Endocytosis by random initiation and stabilization of clathrin-coated pits
    Marcelo Ehrlich
    Department of Cell Biology, Harvard Medical School and the CBR Institute for Biomedical Research, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell 118:591-605. 2004
    ..Cargo incorporation occurs primarily or exclusively in a newly formed coated pit. Our data lead to a model in which coated pits initiate randomly but collapse unless stabilized, perhaps by cargo capture...
  24. ncbi request reprint Possible role of deep tubular invaginations of the plasma membrane in MHC-I trafficking
    Ramiro H Massol
    Department of Cell Biology and The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Exp Cell Res 306:142-9. 2005
    ..Finally, live-cell imaging also shows internalization of MHC-I molecules into these tubules, suggesting that this is an additional route for MHC-I traffic...

Research Grants24

  1. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2010
    ..By the end of this grant period, we aim to be able to study the dynamics of clathrin coat assembly and disassembly in a reconstituted system, in parallel with our work on coat dynamics in living cells. ..
  2. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2006
    ..abstract_text> ..
  3. Dynamics of Clathrin Coat Formation in Cells
    Tomas Kirchhausen; Fiscal Year: 2007
    ....
  4. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2007
    ..By the end of this grant period, we aim to be able to study the dynamics of clathrin coat assembly and disassembly in a reconstituted system, in parallel with our work on coat dynamics in living cells. ..
  5. MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLES
    Tomas Kirchhausen; Fiscal Year: 2002
    ..We thus hope to provide a molecular picture of the key steps in sorting and coat assembly. ..
  6. STRUCTURAL CHEMISTRY OF CLATHRIN & ASSOCIATED PROTEINS
    Tomas Kirchhausen; Fiscal Year: 1993
    ..We will modify DNA sequences and transfect cells in an effort to influence aspects of coat formation and function...