Research Topics
Genomes and Genes | TOMAS L KIRCHHAUSENSummaryAffiliation: Harvard University Country: USA Publications
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Publications
Cholera toxin toxicity does not require functional Arf6- and dynamin-dependent endocytic pathwaysRamiro H Massol
Department of Cell Biology, Harvard Medical School and The Center for Blood Research for Biomedical Research, Boston, Massachusetts 02115, USA
Mol Biol Cell 15:3631-41. 2004..These results are consistent with the existence of an additional retrograde pathway used by CT to reach the ER...
Bending membranesTom Kirchhausen
Department of Cell Biology, Harvard Medical School and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, Massachusetts 02115, USA
Nat Cell Biol 14:906-8. 2012..The second reports that membrane crowding, driven by protein-protein interactions, can also drive membrane bending, even in the absence of any protein insertion into the bilayer...- Limited transferrin receptor clustering allows rapid diffusion of canine parvovirus into clathrin endocytic structuresDavid K Cureton
Immune Disease Institute and Program in Cellular and Molecular Medicine at Children s Hospital Boston, Boston, Massachusetts, USA
J Virol 86:5330-40. 2012.... - Imaging endocytic clathrin structures in living cellsTom Kirchhausen
Harvard Medical School Immune Disease Institute, W Alpert Building Room 128, Boston, MA 02115, USA
Trends Cell Biol 19:596-605. 2009..Here, I outline descriptions of coat assembly in different circumstances that are consistent with what is known from X-ray crystallography and electron microscopy... 
Making COPII coatsTomas Kirchhausen
Department of Cell Biology, Harvard Medical School, Boston, MA 02115 5701, USA
Cell 129:1251-2. 2007..Their findings suggest a mechanism by which the COPII cage assembles and accommodates cargo of different sizes...- Wiskott-Aldrich syndrome: a gene, a multifunctional protein and the beginnings of an explanationT Kirchhausen
Center for Blood Research, Boston, MA 02115, USA
Mol Med Today 4:300-4. 1998..Thus, WASP appears to integrate these two basic and fundamental cellular mechanisms. Several groups are now focusing on understanding the function of WASP in detail, and translating this new knowledge into improved therapies... - ClathrinT Kirchhausen
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Annu Rev Biochem 69:699-727. 2000..This review summarizes and illustrates the recent structural results and outlines what is known about coated-vesicle assembly in the context of this information... - Adaptors for clathrin-mediated trafficT Kirchhausen
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
Annu Rev Cell Dev Biol 15:705-32. 1999..This review focuses on the most recent advances dealing with the molecular basis for sorting by clathrin adaptors... - Three ways to make a vesicleT Kirchhausen
Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
Nat Rev Mol Cell Biol 1:187-98. 2000..A general model to explain the formation of coated vesicles is starting to emerge but the picture is more complex than we had imagined... - Vesicle formation: dynamic dynamin lives up to its nameT Kirchhausen
Department of Cell Biology and Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
Curr Biol 8:R792-4. 1998.... - Exo1: a new chemical inhibitor of the exocytic pathwayYan Feng
Institute of Chemistry and Cell Biology, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:6469-74. 2003..Thus, Exo1 allows the fatty acid exchange activity of Bars50 to be distinguished from ARF1 activity in the control of Golgi tubulation... - Retrograde transport of cholera toxin from the plasma membrane to the endoplasmic reticulum requires the trans-Golgi network but not the Golgi apparatus in Exo2-treated cellsYan Feng
Department of Cell Biology, Institute of Chemistry and Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA
EMBO Rep 5:596-601. 2004..We propose that even in the absence of Exo2 the glycolipid pathway that carries the toxin from plasma membrane into the ER bypasses the Golgi apparatus entirely... - Chemical genetic screening identifies sulfonamides that raise organellar pH and interfere with membrane trafficThomas J F Nieland
Harvard Medical School, Department of Cell Biology and The CBR Institute for Biomedical Research, 200 Longwood Avenue, Boston MA, 02115, USA
Traffic 5:478-92. 2004..Identification of this novel class of sulfonamide inhibitors provides new chemical tools to better understand the function of organelle pH in membrane traffic and the activity of V-ATPases in particular... - Vesicular stomatitis virus enters cells through vesicles incompletely coated with clathrin that depend upon actin for internalizationDavid K Cureton
Departments of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Pathog 5:e1000394. 2009..This work provides new mechanistic insights into the process of virus internalization as well as uptake of unconventional cargo by the clathrin-dependent endocytic machinery... - HIV Nef-mediated major histocompatibility complex class I down-modulation is independent of Arf6 activityJakob E Larsen
Department of Cell Biology and The Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA
Mol Biol Cell 15:323-31. 2004..We conclude that the apparent Arf6 dependency of Nef-mediated MHC class I down-modulation is due to nonspecific perturbations in membrane traffic... - Endosomal recycling controls plasma membrane area during mitosisEmmanuel Boucrot
Department of Cell Biology and CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:7939-44. 2007..We propose a mechanism by which modulation of endosomal recycling controls cell area and surface expression of membrane-bound proteins during cell division... - Distinct dynamics of endocytic clathrin-coated pits and coated plaquesSaveez Saffarian
Department of Cell Biology, Harvard Medical School, Children s Hospital and Immune Disease Institute, Boston, Massachusetts, USA
PLoS Biol 7:e1000191. 2009.... - Discovery of chemical inhibitors of the selective transfer of lipids mediated by the HDL receptor SR-BIThomas J F Nieland
Department of Cell Biology, Harvard Medical School, and Center for Blood Research, Boston, MA 02115 5701, USA
Proc Natl Acad Sci U S A 99:15422-7. 2002.... - Differential evanescence nanometry: live-cell fluorescence measurements with 10-nm axial resolution on the plasma membraneSaveez Saffarian
Department of Cell Biology and Immune Disease Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Biophys J 94:2333-42. 2008..We find a gradually increasing axial separation between the centroids of clathrin and AP-2 distribution, up to a final value of 30 nm just before coated-pit pinching and formation of the coated vesicle... - Targeting of AMSH to endosomes is required for epidermal growth factor receptor degradationYu May Ma
CBR Institute for Biomedical Research, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 282:9805-12. 2007.... - Association of Dishevelled with the clathrin AP-2 adaptor is required for Frizzled endocytosis and planar cell polarity signalingAnan Yu
Department of Cell Biology and CBR Institute for Biomedical Research, Harvard Medical School, Boston, MA 02115, USA
Dev Cell 12:129-41. 2007..We suggest that the direct interaction of Dvl2 with AP-2 is important for Frizzled internalization and Frizzled/PCP signaling... - Secramine inhibits Cdc42-dependent functions in cells and Cdc42 activation in vitroHenry E Pelish
Department of Cell Biology and The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
Nat Chem Biol 2:39-46. 2006..RhoGDI-dependent Cdc42 inhibition by secramine illustrates a new way to inhibit Rho GTPases with small molecules and provides a new means to study Cdc42, RhoGDI and the cellular processes they mediate... - Endocytosis by random initiation and stabilization of clathrin-coated pitsMarcelo Ehrlich
Department of Cell Biology, Harvard Medical School and the CBR Institute for Biomedical Research, 200 Longwood Avenue, Boston, MA 02115, USA
Cell 118:591-605. 2004..Cargo incorporation occurs primarily or exclusively in a newly formed coated pit. Our data lead to a model in which coated pits initiate randomly but collapse unless stabilized, perhaps by cargo capture... - Possible role of deep tubular invaginations of the plasma membrane in MHC-I traffickingRamiro H Massol
Department of Cell Biology and The CBR Institute for Biomedical Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Exp Cell Res 306:142-9. 2005..Finally, live-cell imaging also shows internalization of MHC-I molecules into these tubules, suggesting that this is an additional route for MHC-I traffic...
Research Grants
- MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLESTomas Kirchhausen; Fiscal Year: 2010..By the end of this grant period, we aim to be able to study the dynamics of clathrin coat assembly and disassembly in a reconstituted system, in parallel with our work on coat dynamics in living cells. ..
- MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLESTomas Kirchhausen; Fiscal Year: 2006..abstract_text> ..
- Dynamics of Clathrin Coat Formation in CellsTomas Kirchhausen; Fiscal Year: 2007....
- MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLESTomas Kirchhausen; Fiscal Year: 2007..By the end of this grant period, we aim to be able to study the dynamics of clathrin coat assembly and disassembly in a reconstituted system, in parallel with our work on coat dynamics in living cells. ..
- MOLECULAR INTERACTIONS IN CLATHRIN COATED VESICLESTomas Kirchhausen; Fiscal Year: 2002..We thus hope to provide a molecular picture of the key steps in sorting and coat assembly. ..
- STRUCTURAL CHEMISTRY OF CLATHRIN & ASSOCIATED PROTEINSTomas Kirchhausen; Fiscal Year: 1993..We will modify DNA sequences and transfect cells in an effort to influence aspects of coat formation and function...