Tom Kirchhausen

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Single-handed recognition of a sorting traffic motif by the GGA proteins
    Tom Kirchhausen
    Nat Struct Biol 9:241-4. 2002
  2. pmc Use of dynasore, the small molecule inhibitor of dynamin, in the regulation of endocytosis
    Tom Kirchhausen
    Department of Cell Biology, Harvard Medical School, and IDI Immune Research Institute, Boston, Massachusetts, USA
    Methods Enzymol 438:77-93. 2008
  3. ncbi request reprint Clathrin adaptors really adapt
    Tom Kirchhausen
    Department of Cell Biology, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA
    Cell 109:413-6. 2002
  4. pmc Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriers
    Comert Kural
    Department of Cell Biology, Harvard Medical School, Boston and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, MA 02115, USA
    Cell Rep 2:1111-9. 2012
  5. pmc Clathrin-mediated endocytosis persists during unperturbed mitosis
    Silvia K Tacheva-Grigorova
    Program in Cellular and Molecular Medicine, Boston Children s Hospital, Boston, MA 02115, USA
    Cell Rep 4:659-68. 2013
  6. pmc The small molecule dispergo tubulates the endoplasmic reticulum and inhibits export
    Lei Lu
    Department of Cell Biology, Harvard Medical School, MA, USA
    Mol Biol Cell 24:1020-9. 2013
  7. pmc The first five seconds in the life of a clathrin-coated pit
    Emanuele Cocucci
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 150:495-507. 2012
  8. pmc Imaging endocytic clathrin structures in living cells
    Tom Kirchhausen
    Harvard Medical School Immune Disease Institute, W Alpert Building Room 128, Boston, MA 02115, USA
    Trends Cell Biol 19:596-605. 2009
  9. ncbi request reprint Bending membranes
    Tom Kirchhausen
    Department of Cell Biology, Harvard Medical School and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Cell Biol 14:906-8. 2012
  10. pmc Limited transferrin receptor clustering allows rapid diffusion of canine parvovirus into clathrin endocytic structures
    David K Cureton
    Immune Disease Institute and Program in Cellular and Molecular Medicine at Children s Hospital Boston, Boston, Massachusetts, USA
    J Virol 86:5330-40. 2012

Collaborators

  • Lei Lu
  • Steeve Boulant
  • Emmanuel Boucrot
  • Xiaobo Zhou
  • Deborah Krakow
  • Noelle D Dwyer
  • Jennifer L Moran
  • François Aguet
  • Paul L McNeil
  • Venkatesh Murthy
  • Rami N Hannoush
  • Daniel H Cohn
  • Emanuele Cocucci
  • Comert Kural
  • Silvia K Tacheva-Grigorova
  • Shan Jiang
  • Till Böcking
  • David K Cureton
  • Kazuya Hori
  • Patrick Smits
  • Stephen T C Wong
  • A Jamila Newton
  • Olivia Paleotti
  • Jean Christophe Zeeh
  • Marianne Boes
  • Iris Rapoport
  • Manuel Banzhaf
  • Anan Yu
  • António J M Santos
  • Carole E Harbison
  • Delfim Duarte
  • Thorsten Baust
  • Colin R Parrish
  • Anindya Sen
  • Spyros Artavanis-Tsakonas
  • Barry Merriman
  • Mary Prysak
  • Vincent Funari
  • Matthew L Warman
  • Andrea Superti-Furga
  • Stanley F Nelson
  • Eric D Boyden
  • Minh Hong
  • David R Beier
  • Andrew D Bolton
  • Shiro Ikegawa
  • Luisa Bonafe
  • Danielle K Manning
  • Mariagrazia Partisani
  • Pierre Chardin
  • Frederic Luton
  • Stephanie Klein
  • Michel Franco
  • Eric Macia
  • Ramiro Massol
  • Jan Cerny
  • Jianzhu Chen
  • Hidde L Ploegh
  • Marjolein Op den Brouw

Detail Information

Publications21

  1. ncbi request reprint Single-handed recognition of a sorting traffic motif by the GGA proteins
    Tom Kirchhausen
    Nat Struct Biol 9:241-4. 2002
  2. pmc Use of dynasore, the small molecule inhibitor of dynamin, in the regulation of endocytosis
    Tom Kirchhausen
    Department of Cell Biology, Harvard Medical School, and IDI Immune Research Institute, Boston, Massachusetts, USA
    Methods Enzymol 438:77-93. 2008
    ..Here we present a detailed synthesis protocol for dynasore, and describe a series of experiments used to analyze the inhibitory effects of dynasore on dynamin in vitro and to study the effects of dynasore on endocytosis in cells...
  3. ncbi request reprint Clathrin adaptors really adapt
    Tom Kirchhausen
    Department of Cell Biology, Harvard Medical School and The Center for Blood Research, Boston, MA 02115, USA
    Cell 109:413-6. 2002
    ..A paper in this issue of Cell describes the atomic structure of the adaptor-protein 2 (AP-2) core, the portion that makes contacts with the membrane and cytosolic tails of cargo proteins...
  4. pmc Dynamics of intracellular clathrin/AP1- and clathrin/AP3-containing carriers
    Comert Kural
    Department of Cell Biology, Harvard Medical School, Boston and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, MA 02115, USA
    Cell Rep 2:1111-9. 2012
    ..This observation suggests that an alternative budding mechanism, yet to be discovered, is responsible for the scission step of clathrin/AP1 and clathrin/AP3 carriers...
  5. pmc Clathrin-mediated endocytosis persists during unperturbed mitosis
    Silvia K Tacheva-Grigorova
    Program in Cellular and Molecular Medicine, Boston Children s Hospital, Boston, MA 02115, USA
    Cell Rep 4:659-68. 2013
    ..Thus, our data show that endocytosis is unaffected during all stages of natural cell division. ..
  6. pmc The small molecule dispergo tubulates the endoplasmic reticulum and inhibits export
    Lei Lu
    Department of Cell Biology, Harvard Medical School, MA, USA
    Mol Biol Cell 24:1020-9. 2013
    ..Because dispergo is the first small molecule that reversibly tubulates the ER and inhibits its export function, it will be useful in studying these complex processes...
  7. pmc The first five seconds in the life of a clathrin-coated pit
    Emanuele Cocucci
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Cell 150:495-507. 2012
    ..This objective picture of coated pit initiation also shows that methods outlined here will be broadly useful for studies of dynamic assemblies in living cells...
  8. pmc Imaging endocytic clathrin structures in living cells
    Tom Kirchhausen
    Harvard Medical School Immune Disease Institute, W Alpert Building Room 128, Boston, MA 02115, USA
    Trends Cell Biol 19:596-605. 2009
    ..Here, I outline descriptions of coat assembly in different circumstances that are consistent with what is known from X-ray crystallography and electron microscopy...
  9. ncbi request reprint Bending membranes
    Tom Kirchhausen
    Department of Cell Biology, Harvard Medical School and Program in Cellular and Molecular Medicine at Boston Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Cell Biol 14:906-8. 2012
    ..The second reports that membrane crowding, driven by protein-protein interactions, can also drive membrane bending, even in the absence of any protein insertion into the bilayer...
  10. pmc Limited transferrin receptor clustering allows rapid diffusion of canine parvovirus into clathrin endocytic structures
    David K Cureton
    Immune Disease Institute and Program in Cellular and Molecular Medicine at Children s Hospital Boston, Boston, Massachusetts, USA
    J Virol 86:5330-40. 2012
    ....
  11. pmc Live-cell imaging of clathrin coats
    Comert Kural
    Department of Cell Biology, Harvard Medical School, and Immune Disease Institute and Program in Cellular and Molecular Medicine at Children s Hospital, Boston, Massachusetts, USA
    Methods Enzymol 505:59-80. 2012
    ....
  12. pmc Cisternal organization of the endoplasmic reticulum during mitosis
    Lei Lu
    Department of Cell Biology and Immune Disease Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Biol Cell 20:3471-80. 2009
    ..In contrast, during interphase, the ER displays the familiar reticular network of convolved cisternae linked to tubules...
  13. pmc Key interactions for clathrin coat stability
    Till Böcking
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA Centre for Vascular Research, University of New South Wales, Sydney NSW 2052 Australia Electronic address
    Structure 22:819-29. 2014
    ..Coats destabilized by these mutations require fewer Hsc70 molecules to initiate disassembly, as predicted by a model in which Hsc70 traps conformational distortions during the auxilin- and Hsc70:ATP-mediated uncoating reaction. ..
  14. pmc Synergy between the ESCRT-III complex and Deltex defines a ligand-independent Notch signal
    Kazuya Hori
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Biol 195:1005-15. 2011
    ..This activation mode of the receptor emphasizes the complexity of Notch signal modulation in a cell and has significant implications for both development and disease...
  15. pmc Lethal skeletal dysplasia in mice and humans lacking the golgin GMAP-210
    Patrick Smits
    Orthopedic Research Laboratories, Department of Orthopedic Surgery, Children s Hospital, Boston, MA 02115, USA
    N Engl J Med 362:206-16. 2010
    ..Establishing the genetic basis of phenotypes such as skeletal dysplasia in model organisms can provide insights into biologic processes and their role in human disease...
  16. ncbi request reprint Tracking molecular particles in live cells using fuzzy rule-based system
    Shan Jiang
    HCNR Center for Bioinformatics, Harvard Medical School and Brigham and Women s Hospital, Boston, Massachusetts 02215, USA
    Cytometry A 71:576-84. 2007
    ..Results show that this method can accurately track most of the particles in the high volume images...
  17. ncbi request reprint T-cell engagement of dendritic cells rapidly rearranges MHC class II transport
    Marianne Boes
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 418:983-8. 2002
    ..We propose that such tubulation serves to facilitate the ensuing T-cell response...
  18. ncbi request reprint The small G-protein Arf6GTP recruits the AP-2 adaptor complex to membranes
    Olivia Paleotti
    Institut de Pharmacologie Moleculaire et Cellulaire, CNRS Unité Mixte de Recherche 6097, 660 route des Lucioles, 06560 Valbonne Sophia Antipolis, France
    J Biol Chem 280:21661-6. 2005
    ..These findings strongly suggest that Arf6 plays a major role in clathrin-mediated endocytosis by directly controlling the assembly of the AP-2/clathrin coat...
  19. ncbi request reprint Detection of molecular particles in live cells via machine learning
    Shan Jiang
    Department of Mathematical Sciences, Tsinghua University, Beijing, 100084, People s Republic of China
    Cytometry A 71:563-75. 2007
    ..In order to get the accurate boundaries of these particles, several post-processing methods are applied and signal-to-noise ratio analysis is also performed to rule out the weak spots...
  20. ncbi request reprint An emergency response team for membrane repair
    Paul L McNeil
    Department of Anatomy and Cellular Biology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30921, USA
    Nat Rev Mol Cell Biol 6:499-505. 2005
    ....
  21. pmc Inhibition of dynamin completely blocks compensatory synaptic vesicle endocytosis
    A Jamila Newton
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 103:17955-60. 2006
    ..In sum, our data indicate that dynamin is essential for all forms of compensatory synaptic vesicle endocytosis including any kiss-and-run events...