Donald E Ingber

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Paxillin mediates sensing of physical cues and regulates directional cell motility by controlling lamellipodia positioning
    Julia E Sero
    Vascular Biology Program, Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 6:e28303. 2011
  2. pmc How changes in extracellular matrix mechanics and gene expression variability might combine to drive cancer progression
    Justin Werfel
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, United States of America
    PLoS ONE 8:e76122. 2013
  3. ncbi request reprint A microdevice for rapid optical detection of magnetically captured rare blood pathogens
    Ryan M Cooper
    Wyss Institute for Biologically Inspired Engineering at Harvard University, 3 Blackfan Circle, CLSB 517, Boston, MA 02118, USA
    Lab Chip 14:182-8. 2014
  4. doi request reprint Mechanobiology and developmental control
    Tadanori Mammoto
    Vascular Biology Program, Boston Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115 email
    Annu Rev Cell Dev Biol 29:27-61. 2013
  5. pmc Clear castable polyurethane elastomer for fabrication of microfluidic devices
    Karel Domansky
    Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA
    Lab Chip 13:3956-64. 2013
  6. doi request reprint Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation
    Hyun Jung Kim
    Wyss Institute for Biologically Inspired Engineering at Harvard University, CLSB Bldg 5th floor, 3 Blackfan Circle, Boston, MA 02115, USA
    Integr Biol (Camb) 5:1130-40. 2013
  7. doi request reprint Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment
    Kyung Jin Jang
    Wyss Institute for Biologically Inspired Engineering at Harvard University, CLSB Bldg 5th floor, 3 Blackfan Circle, Boston, MA 02115, USA
    Integr Biol (Camb) 5:1119-29. 2013
  8. pmc From cellular mechanotransduction to biologically inspired engineering: 2009 Pritzker Award Lecture, BMES Annual Meeting October 10, 2009
    Donald E Ingber
    Wyss Institute for Biologically Inspired Engineering, Harvard University, and Department of Pathology, Children s Hospital, Boston, MA 02115 5737, USA
    Ann Biomed Eng 38:1148-61. 2010
  9. pmc Tumor-derived endothelial cells exhibit aberrant Rho-mediated mechanosensing and abnormal angiogenesis in vitro
    Kaustabh Ghosh
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 105:11305-10. 2008
  10. ncbi request reprint Mechanical forces alter zyxin unbinding kinetics within focal adhesions of living cells
    Tanmay P Lele
    Department of Surgery, Vascular Biology Program, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Physiol 207:187-94. 2006

Collaborators

Detail Information

Publications105 found, 100 shown here

  1. pmc Paxillin mediates sensing of physical cues and regulates directional cell motility by controlling lamellipodia positioning
    Julia E Sero
    Vascular Biology Program, Department of Pathology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 6:e28303. 2011
    ..These findings also suggest that CDRs may correspond to invasive protrusions that drive cell migration through 3D extracellular matrices...
  2. pmc How changes in extracellular matrix mechanics and gene expression variability might combine to drive cancer progression
    Justin Werfel
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, United States of America
    PLoS ONE 8:e76122. 2013
    ..g., altered ECM mechanics) to induce a cancerous phenotype or accelerate cancer progression in a clonal population through local changes in cell geometry and increased phenotypic variability, even in the absence of gene mutation. ..
  3. ncbi request reprint A microdevice for rapid optical detection of magnetically captured rare blood pathogens
    Ryan M Cooper
    Wyss Institute for Biologically Inspired Engineering at Harvard University, 3 Blackfan Circle, CLSB 517, Boston, MA 02118, USA
    Lab Chip 14:182-8. 2014
    ..Using this device, we have been able to detect fungal pathogens in less than three hours after sample collection compared to days with current technology, and with an extremely high sensitivity (<1 cell mL(-1) of human blood). ..
  4. doi request reprint Mechanobiology and developmental control
    Tadanori Mammoto
    Vascular Biology Program, Boston Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115 email
    Annu Rev Cell Dev Biol 29:27-61. 2013
    ....
  5. pmc Clear castable polyurethane elastomer for fabrication of microfluidic devices
    Karel Domansky
    Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA
    Lab Chip 13:3956-64. 2013
    ..Here we describe an attractive alternative material that is similar to PDMS in terms of optical transparency, flexibility and castability, but that is also resistant to absorption of small hydrophobic molecules. ..
  6. doi request reprint Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation
    Hyun Jung Kim
    Wyss Institute for Biologically Inspired Engineering at Harvard University, CLSB Bldg 5th floor, 3 Blackfan Circle, Boston, MA 02115, USA
    Integr Biol (Camb) 5:1130-40. 2013
    ....
  7. doi request reprint Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment
    Kyung Jin Jang
    Wyss Institute for Biologically Inspired Engineering at Harvard University, CLSB Bldg 5th floor, 3 Blackfan Circle, Boston, MA 02115, USA
    Integr Biol (Camb) 5:1119-29. 2013
    ..The in vivo-like pathophysiology observed in this system suggests that it might serve as a useful tool for evaluating human-relevant renal toxicity in preclinical safety studies. ..
  8. pmc From cellular mechanotransduction to biologically inspired engineering: 2009 Pritzker Award Lecture, BMES Annual Meeting October 10, 2009
    Donald E Ingber
    Wyss Institute for Biologically Inspired Engineering, Harvard University, and Department of Pathology, Children s Hospital, Boston, MA 02115 5737, USA
    Ann Biomed Eng 38:1148-61. 2010
    ....
  9. pmc Tumor-derived endothelial cells exhibit aberrant Rho-mediated mechanosensing and abnormal angiogenesis in vitro
    Kaustabh Ghosh
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115
    Proc Natl Acad Sci U S A 105:11305-10. 2008
    ....
  10. ncbi request reprint Mechanical forces alter zyxin unbinding kinetics within focal adhesions of living cells
    Tanmay P Lele
    Department of Surgery, Vascular Biology Program, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Physiol 207:187-94. 2006
    ....
  11. pmc Mechanochemical control of mesenchymal condensation and embryonic tooth organ formation
    Tadanori Mammoto
    Vascular Biology Program, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 21:758-69. 2011
    ....
  12. pmc TRPV4 channels mediate cyclic strain-induced endothelial cell reorientation through integrin-to-integrin signaling
    Charles K Thodeti
    Vascular Biology Program, Department of Surgery, 300 Longwood Avenue, Children s Hospital Harvard Medical School, Boston, MA 02115, USA
    Circ Res 104:1123-30. 2009
    ....
  13. ncbi request reprint Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension
    Kimberly A Moore
    Vascular Biology Program, Department of Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Dev Dyn 232:268-81. 2005
    ....
  14. ncbi request reprint Global cytoskeletal control of mechanotransduction in kidney epithelial cells
    Francis J Alenghat
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Exp Cell Res 301:23-30. 2004
    ....
  15. ncbi request reprint Breast cancer normalization induced by embryonic mesenchyme is mediated by extracellular matrix biglycan
    Ashley G Bischof
    Vascular Biology Program, Boston Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Integr Biol (Camb) 5:1045-56. 2013
    ..These studies confirm that embryonic mesenchyme retains the ability to induce partial breast cancer reversion, and that its inductive capability resides at least in part in the ECM protein biglycan that it produces...
  16. pmc Ultra-rapid activation of TRPV4 ion channels by mechanical forces applied to cell surface beta1 integrins
    Benjamin D Matthews
    Department of Medicine, Harvard Medical School and Children s Hospital, Boston, MA 02115, USA
    Integr Biol (Camb) 2:435-42. 2010
    ....
  17. ncbi request reprint Nanostructured magnetizable materials that switch cells between life and death
    Thomas R Polte
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, MA, USA
    Biomaterials 28:2783-90. 2007
    ....
  18. ncbi request reprint Ablation of cytoskeletal filaments and mitochondria in live cells using a femtosecond laser nanoscissor
    Nan Shen
    Department of Physics, Harvard University, USA
    Mech Chem Biosyst 2:17-25. 2005
    ..Using this approach, we unequivocally demonstrate that mitochondria are structurally independent functional units, and do not form a continuous network as suggested by some past studies...
  19. pmc Viscoelastic retraction of single living stress fibers and its impact on cell shape, cytoskeletal organization, and extracellular matrix mechanics
    Sanjay Kumar
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115 5737, USA
    Biophys J 90:3762-73. 2006
    ....
  20. ncbi request reprint Directional control of lamellipodia extension by constraining cell shape and orienting cell tractional forces
    Kevin Kit Parker
    Department of Pathology and Surgery, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    FASEB J 16:1195-204. 2002
    ..When cell tension was dissipated, lamellipodia extension ceased. Mechanical interactions between cells and ECM that modulate cytoskeletal tension may therefore play a key role in the control of directional cell motility...
  21. ncbi request reprint Magnetically-guided self-assembly of fibrin matrices with ordered nano-scale structure for tissue engineering
    Eben Alsberg
    Vascular Biology Program, Children s Hospital Harvard Medical School, Boston, Massachusetts 02115 5737, USA
    Tissue Eng 12:3247-56. 2006
    ..This magnetically-guided, biologically-inspired microfabrication system is unique in that large scaffolds may be formed with little starting material, and thus it may be useful for in vivo tissue engineering applications in the future...
  22. pmc A mechanosensitive transcriptional mechanism that controls angiogenesis
    Akiko Mammoto
    Vascular Biology Program, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 457:1103-8. 2009
    ..This is, to our knowledge, the first known functional cross-antagonism between transcription factors that controls tissue morphogenesis, and that responds to both mechanical and chemical cues...
  23. ncbi request reprint Tools to study cell mechanics and mechanotransduction
    Tanmay P Lele
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Methods Cell Biol 83:443-72. 2007
    ..In this chapter, we describe techniques like microcontact printing, magnetic twisting cytometry, and magnetic pulling cytometry that can be systematically used to study the molecular basis of cellular mechanotransduction...
  24. doi request reprint Reconstituting organ-level lung functions on a chip
    Dongeun Huh
    Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA
    Science 328:1662-8. 2010
    ....
  25. doi request reprint Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics
    Akiko Mammoto
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Commun 4:1759. 2013
    ....
  26. doi request reprint A human disease model of drug toxicity-induced pulmonary edema in a lung-on-a-chip microdevice
    Dongeun Huh
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
    Sci Transl Med 4:159ra147. 2012
    ....
  27. doi request reprint Methods for studying mechanical control of angiogenesis by the cytoskeleton and extracellular matrix
    Akiko Mammoto
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Methods Enzymol 443:227-59. 2008
    ..These techniques are useful as in vitro angiogenesis models and for analyzing the molecular and biophysical basis of vascular control...
  28. ncbi request reprint Geometric determinants of directional cell motility revealed using microcontact printing
    Amy Brock
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital, Harvard Medical School, Boston, Massachusetts
    Langmuir 19:1611-7. 2003
    ....
  29. doi request reprint Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation
    Nan Xia
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    FASEB J 22:1649-59. 2008
    ....
  30. ncbi request reprint Extracellular matrix controls myosin light chain phosphorylation and cell contractility through modulation of cell shape and cytoskeletal prestress
    Thomas R Polte
    Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Am J Physiol Cell Physiol 286:C518-28. 2004
    ....
  31. pmc ABL2/ARG tyrosine kinase mediates SEMA3F-induced RhoA inactivation and cytoskeleton collapse in human glioma cells
    Akio Shimizu
    Vascular Biology Program, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 283:27230-8. 2008
    ..Cells treated with p190RhoGAP small interfering RNA also did not inactivate RhoA. Together, these results suggested that ABL2/ARG is a novel mediator of SEMA3F-induced RhoA inactivation and collapsing activity...
  32. pmc Mechanosensitive mechanisms in transcriptional regulation
    Akiko Mammoto
    Vascular Biology Program, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    J Cell Sci 125:3061-73. 2012
    ..A deeper understanding of these mechanosensitive transcriptional control mechanisms should lead to new approaches to tissue engineering and regenerative medicine...
  33. pmc Rheological behavior of living cells is timescale-dependent
    Dimitrije Stamenovic
    Department of Biomedical Engineering, Boston University, and Department of Pathology, Children s Hospital, Boston, Massachusetts, USA
    Biophys J 93:L39-41. 2007
    ....
  34. doi request reprint Inhibition of mammary tumor growth using lysyl oxidase-targeting nanoparticles to modify extracellular matrix
    Mathumai Kanapathipillai
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, United States
    Nano Lett 12:3213-7. 2012
    ..This nanomaterials approach opens a new path for treating cancer with higher efficacy and decreased side effects...
  35. ncbi request reprint Mechanical properties of individual focal adhesions probed with a magnetic microneedle
    Benjamin D Matthews
    Vascular Biology Program, Department of Pathology, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Biochem Biophys Res Commun 313:758-64. 2004
    ....
  36. ncbi request reprint Microfabrication of human organs-on-chips
    Dongeun Huh
    1 Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, Massachusetts, USA 2 Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Nat Protoc 8:2135-57. 2013
    ..Also, we discuss experimental techniques that can be used to analyze the cells in these organ-on-chip devices. ..
  37. ncbi request reprint Loss of polycystin-1 in human cyst-lining epithelia leads to ciliary dysfunction
    Surya M Nauli
    Harvard Institutes of Medicine, Suite 520, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    J Am Soc Nephrol 17:1015-25. 2006
    ..This report proposes that calcium response to fluid-flow shear stress can be used as a readout of polycystin function and that loss of mechanosensation in the renal tubular epithelia is a feature of PKD cysts...
  38. ncbi request reprint Caldesmon-dependent switching between capillary endothelial cell growth and apoptosis through modulation of cell shape and contractility
    Yasushi Numaguchi
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Angiogenesis 6:55-64. 2003
    ....
  39. pmc Tensegrity-based mechanosensing from macro to micro
    Donald E Ingber
    Vascular Biology Program, Department of Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115 5737, USA
    Prog Biophys Mol Biol 97:163-79. 2008
    ....
  40. pmc Epoxyeicosanoids promote organ and tissue regeneration
    Dipak Panigrahy
    Vascular Biology Program and Department of Surgery, Boston Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:13528-33. 2013
    ..Thus, our observations indicate a central role for EETs in organ and tissue regeneration and their contribution to tissue homeostasis. ..
  41. ncbi request reprint Alpha-actinin-4 is required for normal podocyte adhesion
    Savita V Dandapani
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
    J Biol Chem 282:467-77. 2007
    ..These results suggest that alpha-actinin-4 interacts with integrins and strengthens the podocyte-GBM interaction thereby stabilizing glomerular architecture and preventing disease...
  42. pmc LRP5 regulates development of lung microvessels and alveoli through the angiopoietin-Tie2 pathway
    Tadanori Mammoto
    Vascular Biology Program, Department of Surgery, Boston Children s Hospital and Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 7:e41596. 2012
    ....
  43. ncbi request reprint Force meets chemistry: analysis of mechanochemical conversion in focal adhesions using fluorescence recovery after photobleaching
    Tanmay P Lele
    Vascular Biology Program, Department of Pathology and Surgery, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biochem 97:1175-83. 2006
    ..It also may facilitate future efforts to bridge biological experimentation and mathematical modeling in our quest for a systems biology level description of cell regulation...
  44. doi request reprint A combined micromagnetic-microfluidic device for rapid capture and culture of rare circulating tumor cells
    Joo H Kang
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
    Lab Chip 12:2175-81. 2012
    ....
  45. pmc Multizone paper platform for 3D cell cultures
    Ratmir Derda
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e18940. 2011
    ..This capability made it possible to compare the growth of 3D tumor models of different spatial composition, and to examine the migration of cells in these structures...
  46. doi request reprint Quantifying cell-generated mechanical forces within living embryonic tissues
    Otger Campàs
    1 School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA 2 Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, Massachusetts, USA 3 Vascular Biology Program, Children s Hospital, Boston, Massachusetts, USA 4 Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, USA 5
    Nat Methods 11:183-9. 2014
    ..4 nN μm(-2)) are dependent on myosin II activity and are more than twofold larger than stresses generated by cells of embryonic tooth mesenchyme, either within cultured aggregates or in developing whole mouse mandibles. ..
  47. ncbi request reprint Cellular adaptation to mechanical stress: role of integrins, Rho, cytoskeletal tension and mechanosensitive ion channels
    Benjamin D Matthews
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital, Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 119:508-18. 2006
    ..Thus, cells use multiple mechanisms to sense and respond to static and dynamic changes in the level of mechanical stress applied to integrins...
  48. pmc Mechanical control of cAMP signaling through integrins is mediated by the heterotrimeric Galphas protein
    Francis J Alenghat
    Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biochem 106:529-38. 2009
    ....
  49. ncbi request reprint Discovery of potent and practical antiangiogenic agents inspired by cortistatin A
    Barbara Czakó
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    J Am Chem Soc 131:9014-9. 2009
    ....
  50. doi request reprint Microengineered physiological biomimicry: organs-on-chips
    Dongeun Huh
    Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA
    Lab Chip 12:2156-64. 2012
    ..We also explore challenges that lie ahead if this field is to fulfil its promise to transform the future of drug development and chemical safety testing...
  51. doi request reprint Cytoskeletal control of growth and cell fate switching
    Akiko Mammoto
    Vascular Biology Program, Department of Surgery, Harvard Medical School and Children s Hospital, Boston, MA 02115, USA
    Curr Opin Cell Biol 21:864-70. 2009
    ..This interplay between soluble, insoluble, and mechanical cues helps to explain how cell behaviors responsible for tissue formation are controlled in the physical context of living tissues...
  52. pmc A mini-microscope for in situ monitoring of cells
    Sang Bok Kim
    Center for Biomedical Engineering, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Lab Chip 12:3976-82. 2012
    ..Given its cost effectiveness, robust imaging and portability, the presented platform may be useful for a range of applications for real-time cellular imaging using lab-on-a-chip devices at low cost...
  53. ncbi request reprint Tensegrity II. How structural networks influence cellular information processing networks
    Donald E Ingber
    Department of Surgery, Children s Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 116:1397-408. 2003
    ..In addition, it examines how cell structural networks affect gene and protein signaling networks to produce characteristic phenotypes and cell fate transitions during tissue development...
  54. pmc Paper-supported 3D cell culture for tissue-based bioassays
    Ratmir Derda
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 106:18457-62. 2009
    ..Cell cultures in stacked, paper-supported gels offer a uniquely flexible approach to study cell responses to 3D molecular gradients and to mimic tissue- and organ-level functions...
  55. pmc SLLISWD sequence in the 10FNIII domain initiates fibronectin fibrillogenesis
    Elaine P S Gee
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA
    J Biol Chem 288:21329-40. 2013
    ....
  56. ncbi request reprint Filamin links cell shape and cytoskeletal structure to Rho regulation by controlling accumulation of p190RhoGAP in lipid rafts
    Akiko Mammoto
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 120:456-67. 2007
    ....
  57. pmc Tumor growth and angiogenesis are dependent on the presence of immature dendritic cells
    Ofer Fainaru
    Vascular Biology Program and Department of Surgery, Childrens Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    FASEB J 24:1411-8. 2010
    ..Thus, cancer immunotherapies that promote DC maturation may act by both augmenting the host immune response to the tumor and by suppressing tumor angiogenesis...
  58. ncbi request reprint Methods for measuring rates of protein binding to insoluble scaffolds in living cells: histone H1-chromatin interactions
    Tanmay Lele
    Department of Pathology, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Cell Biochem 99:1334-42. 2006
    ..This work brings clarity to the interpretation of FRAP experiments and provides a way to determine binding kinetics for nuclear proteins and other cellular molecules that interact with insoluble scaffolds within living cells...
  59. ncbi request reprint Cell fates as high-dimensional attractor states of a complex gene regulatory network
    Sui Huang
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Phys Rev Lett 94:128701. 2005
    ....
  60. ncbi request reprint Transcriptome-wide noise controls lineage choice in mammalian progenitor cells
    Hannah H Chang
    Vascular Biology Programme, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 453:544-7. 2008
    ....
  61. doi request reprint Shear-activated nanotherapeutics for drug targeting to obstructed blood vessels
    Netanel Korin
    Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
    Science 337:738-42. 2012
    ....
  62. ncbi request reprint Novel dynamic rheological behavior of individual focal adhesions measured within single cells using electromagnetic pulling cytometry
    Darryl R Overby
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Acta Biomater 1:295-303. 2005
    ..These results are considered in context with the hierarchical nature of cytoskeletal architecture...
  63. ncbi request reprint Role of RhoA, mDia, and ROCK in cell shape-dependent control of the Skp2-p27kip1 pathway and the G1/S transition
    Akiko Mammoto
    Vascular Biology Program, Department of Pathology, Children s Hospital Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:26323-30. 2004
    ....
  64. pmc Fibronectin unfolding revisited: modeling cell traction-mediated unfolding of the tenth type-III repeat
    Elaine P S Gee
    Graduate Program in Biophysics, Harvard University, Cambridge, Massachusetts, USA
    PLoS ONE 3:e2373. 2008
    ..This process enables cells to bind and unfold additional fibronectin modules - a method that propagates matrix assembly...
  65. doi request reprint Rho signaling and mechanical control of vascular development
    Akiko Mammoto
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Opin Hematol 15:228-34. 2008
    ..To discuss how mechanical cues and Rho signaling contribute to control of vascular development and hematopoiesis...
  66. pmc Evidence by molecular profiling for a placental origin of infantile hemangioma
    Carmen M Barnes
    Vascular Biology Program and Department of Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:19097-102. 2005
    ..Furthermore, it suggests that the unique self-limited growth of infantile hemangioma may, in fact, mirror the lifetime of placental endothelium...
  67. ncbi request reprint Tensile forces stimulate vascular remodeling and epidermal cell proliferation in living skin
    Giorgio Pietramaggiori
    Tissue Engineering and Wound Healing Laboratory, Division of Plastic Surgery, Department of Surgery, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Ann Surg 246:896-902. 2007
    ..To quantify tissue remodeling induced by static and cyclical application of tensional forces in a living perfused tissue...
  68. ncbi request reprint A computational tensegrity model predicts dynamic rheological behaviors in living cells
    Cornel Sultan
    Vascular Biology Program, Department of Pathology, Children s Hospital Harvard Medical School, Boston, MA 02115, USA
    Ann Biomed Eng 32:520-30. 2004
    ....
  69. pmc Multistable and multistep dynamics in neutrophil differentiation
    Hannah H Chang
    Vascular Biology Program, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    BMC Cell Biol 7:11. 2006
    ..However, the dynamics of mammalian cell differentiation has not been analyzed with respect to bistability...
  70. pmc Can cancer be reversed by engineering the tumor microenvironment?
    Donald E Ingber
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, MA, United States
    Semin Cancer Biol 18:356-64. 2008
    ....
  71. doi request reprint From 3D cell culture to organs-on-chips
    Dongeun Huh
    Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA
    Trends Cell Biol 21:745-54. 2011
    ....
  72. doi request reprint Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow
    Hyun Jung Kim
    Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA
    Lab Chip 12:2165-74. 2012
    ....
  73. ncbi request reprint Tensegrity I. Cell structure and hierarchical systems biology
    Donald E Ingber
    Department of Surgery, Children s Hospital and Harvard Medical School, Enders 1007, 300 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 116:1157-73. 2003
    ..Part II, which focuses on how these structural networks influence information processing networks, appears in the next issue...
  74. ncbi request reprint Cancer as a disease of epithelial-mesenchymal interactions and extracellular matrix regulation
    Donald E Ingber
    Department of Pathology and Surgery, Harvard Medical School and Children s Hospital, Boston, MA 02115, USA
    Differentiation 70:547-60. 2002
    ..The contributions of integrins, cytoskeleton, tensegrity and local variations in extracellular matrix mechanics to these processes are discussed, as are the implications of this work for future studies on cancer formation...
  75. ncbi request reprint Cellular mechanotransduction: putting all the pieces together again
    Donald E Ingber
    Vascular Biology Program, Karp Family Research Laboratories 11 127, Department of Pathology, Harvard Medical School and Children s Hospital, 300 Longwood Ave, Boston, Massachusetts 02115, USA
    FASEB J 20:811-27. 2006
    ..Future research in this area will therefore require analysis, understanding, and modeling of tensionally integrated (tensegrity) systems of mechanochemical control...
  76. ncbi request reprint Micromechanical control of cell and tissue development: implications for tissue engineering
    Kaustabh Ghosh
    KFRL 11 127, Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Adv Drug Deliv Rev 59:1306-18. 2007
    ....
  77. pmc Mechanosensation through integrins: cells act locally but think globally
    Donald E Ingber
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 100:1472-4. 2003
  78. ncbi request reprint A non-genetic basis for cancer progression and metastasis: self-organizing attractors in cell regulatory networks
    Sui Huang
    Vascular Biology Program, Department of Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Breast Dis 26:27-54. 2006
    ....
  79. ncbi request reprint Mechanobiology and diseases of mechanotransduction
    Donald E Ingber
    Vascular Biology Program, Department of Surgery, Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Ann Med 35:564-77. 2003
    ..Insights into the mechanical basis of tissue regulation also may lead to development of improved medical devices, engineered tissues, and biologically-inspired materials for tissue repair and reconstruction...
  80. ncbi request reprint What lies at the interface of regenerative medicine and developmental biology?
    Donald E Ingber
    Vascular Biology Program, Department of Pathology, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Development 134:2541-7. 2007
    ..These dichotomies raise questions regarding the definition of regenerative medicine, but offer the promise of exciting new interdisciplinary approaches to tissue and organ regeneration, if effective alliances can be established...
  81. ncbi request reprint Mechanical signaling and the cellular response to extracellular matrix in angiogenesis and cardiovascular physiology
    Donald E Ingber
    Vascular Biology Program, Departments of Pathology and Surgery, Harvard Medical School and Children s Hospital, Boston, Mass 02115, USA
    Circ Res 91:877-87. 2002
    ..Thus, the mechanism by which cells perform mechanochemical transduction and the implications of these findings for morphogenetic control are discussed in the wider context of vascular development and cardiovascular physiology...
  82. pmc Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology
    Amy Brock
    Vascular Biology Program, Department of Pathology, Harvard Medical School and Children s Hospital, Enders 1007, 300 Longwood Ave, Boston, MA 02115, USA
    BMC Cell Biol 4:6. 2003
    ....
  83. pmc Mechanical control of tissue growth: function follows form
    Donald E Ingber
    Harvard Medical School and Children s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:11571-2. 2005
  84. ncbi request reprint Micropatterning tractional forces in living cells
    Ning Wang
    Physiology Program, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Cell Motil Cytoskeleton 52:97-106. 2002
    ....
  85. pmc Epoxyeicosanoids stimulate multiorgan metastasis and tumor dormancy escape in mice
    Dipak Panigrahy
    Vascular Biology Program, Children s Hospital Boston, Boston, Massachusetts, USA
    J Clin Invest 122:178-91. 2012
    ..Thus, our data indicate a central role for EETs in tumorigenesis, offering a mechanistic link between lipid signaling and cancer and emphasizing the critical importance of considering possible effects of EET-modulating drugs on cancer...
  86. pmc Mechanical control of tissue and organ development
    Tadanori Mammoto
    Vascular Biology Program, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Development 137:1407-20. 2010
    ....
  87. doi request reprint A Wnt-bmp feedback circuit controls intertissue signaling dynamics in tooth organogenesis
    Daniel J O'Connell
    Division of Genetics, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Sci Signal 5:ra4. 2012
    ....
  88. pmc Tensegrity and mechanotransduction
    Donald E Ingber
    Vascular Biology Program, Department of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115 5737, USA
    J Bodyw Mov Ther 12:198-200. 2008
    ..This structure-based system provides a mechanistic basis to explain how application of physical therapies might influence cell and tissue physiology...
  89. ncbi request reprint A discrete cell cycle checkpoint in late G(1) that is cytoskeleton-dependent and MAP kinase (Erk)-independent
    Sui Huang
    Department of Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Exp Cell Res 275:255-64. 2002
    ....
  90. ncbi request reprint Cell tension, matrix mechanics, and cancer development
    Sui Huang
    Harvard Medical School and Children s Hospital, Boston, Massachusetts 02115, USA
    Cancer Cell 8:175-6. 2005
    ..This mechanical "autocrine loop" brings solid-state mechanotransduction on a par with oncogenic signaling pathways in malignant transformation...
  91. ncbi request reprint Using self-assembled monolayers to pattern ECM proteins and cells on substrates
    Emanuele Ostuni
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA
    Methods Mol Biol 522:183-94. 2009
    ..Neurosci. Res. 13:213-20, 1985; Biotech. Bioeng. 43:792-800, 1994)...
  92. ncbi request reprint Tissue engineering and developmental biology: going biomimetic
    Donald E Ingber
    Vascular Biology Program, Children s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Tissue Eng 12:3265-83. 2006
    ..Collectively, the faculty expressed a remarkable level of enthusiasm for bridging the gaps between developmental biology and tissue engineering and offered new ideas on how to facilitate the interaction between the two fields...
  93. ncbi request reprint Control of embryonic lung branching morphogenesis by the Rho activator, cytotoxic necrotizing factor 1
    Kimberly A Moore
    Department of Surgery, Children s Hospital and Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    J Surg Res 104:95-100. 2002
    ..Since the small GTPase Rho plays an important role in the control of cell tension generation, we carried out studies to test the hypothesis that changes in Rho-mediated cell tension may influence branching morphogenesis...
  94. ncbi request reprint Angiopoietin-1 requires p190 RhoGAP to protect against vascular leakage in vivo
    Tadanori Mammoto
    Department of Medicine, Division of Interdisciplinary Medicine and Biotechnology and Center for Vascular Biology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    J Biol Chem 282:23910-8. 2007
    ..These results provide mechanistic evidence that targeting the endothelium through Tie-2 may offer specific therapeutic strategies in life-threatening endotoxemic conditions such as sepsis and acute respiratory distress syndrome...
  95. ncbi request reprint Mechanical control of tissue morphogenesis during embryological development
    Donald E Ingber
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, MA 02115, USA
    Int J Dev Biol 50:255-66. 2006
    ..Taken together, this work confirms that mechanical forces generated in the cytoskeleton of individual cells and exerted on ECM scaffolds, play a critical role in the sculpting of the embryo...
  96. pmc Polymeric nanomaterials for islet targeting and immunotherapeutic delivery
    Kaustabh Ghosh
    Vascular Biology Program, Departments of Pathology and Surgery, Children s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Nano Lett 12:203-8. 2012
    ..Our findings also underscore the need to carefully tailor drug loading and nanoparticle dosage to achieve maximal vascular targeting and immunosuppression...
  97. pmc Extracellular matrix, mechanotransduction and structural hierarchies in heart tissue engineering
    Kevin K Parker
    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
    Philos Trans R Soc Lond B Biol Sci 362:1267-79. 2007
    ..Through this process, we delineate various design principles that may be useful for engineering artificial heart tissue in the future...
  98. pmc Self-assembly of three-dimensional prestressed tensegrity structures from DNA
    Tim Liedl
    Department of Cancer Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Nanotechnol 5:520-4. 2010
    ..In addition to being building blocks for nanostructures, tensile structural elements made of single-stranded DNA could be used to study molecular forces, cellular mechanotransduction and other fundamental biological processes...
  99. doi request reprint Unexpected strength and toughness in chitosan-fibroin laminates inspired by insect cuticle
    Javier G Fernandez
    Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115, USA
    Adv Mater 24:480-4. 2012
    ..The bioinspired laminate exhibits strength and toughness that are ten times greater than the unstructured component blend and twice that of its strongest constituent...
  100. doi request reprint Micromagnetic-microfluidic blood cleansing device
    Chong Wing Yung
    Vascular Biology Program, Department of Surgery and Pathology, Children s Hospital and Harvard Medical School, 300 Longwood Ave, KFRL 11 127, Boston, MA 02115 5737, USA
    Lab Chip 9:1171-7. 2009
    ....
  101. pmc Microtubules can bear enhanced compressive loads in living cells because of lateral reinforcement
    Clifford P Brangwynne
    Department of Physics, Harvard University, Cambridge, MA 02138, USA
    J Cell Biol 173:733-41. 2006
    ....