CRAIG PATRICK HUNTER

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic patterning genes identifies conserved genetic interactions
    L Ryan Baugh
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Genome Biol 6:R45. 2005
  2. pmc Composition and regulation of maternal and zygotic transcriptomes reflects species-specific reproductive mode
    Shai S Shen-Orr
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA
    Genome Biol 11:R58. 2010
  3. ncbi request reprint Gene silencing: shrinking the black box of RNAi
    C P Hunter
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Curr Biol 10:R137-40. 2000
  4. ncbi request reprint Transport of sequence-specific RNA interference information between cells
    Antony M Jose
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Annu Rev Genet 41:305-30. 2007
  5. ncbi request reprint Transport of dsRNA into cells by the transmembrane protein SID-1
    Evan H Feinberg
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 301:1545-7. 2003
  6. doi request reprint Environmental RNA interference
    Jennifer S Whangbo
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Trends Genet 24:297-305. 2008
  7. pmc Export of RNA silencing from C. elegans tissues does not require the RNA channel SID-1
    Antony M Jose
    Department of Molecular and Cellular Biology, Harvard University, Room 3050, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 106:2283-8. 2009
  8. pmc Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression
    Itai Yanai
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Syst Biol 4:163. 2008
  9. pmc Caenorhabditis elegans SID-2 is required for environmental RNA interference
    William M Winston
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 104:10565-70. 2007
  10. pmc Regulators of the actin cytoskeleton mediate lethality in a Caenorhabditis elegans dhc-1 mutant
    Aleksandra J Gil-Krzewska
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Biol Cell 21:2707-20. 2010

Research Grants

Collaborators

  • L Ryan Baugh
  • Antony M Jose
  • Itai Yanai
  • Shai S Shen-Orr
  • William M Winston
  • Aleksandra J Gil-Krzewska
  • Edgar A Buttner
  • Amanda J Wright
  • Evan H Feinberg
  • Joseph D Shih
  • Marie Sutherlin
  • Jennifer S Whangbo
  • Yuan Qi
  • Daniel H Schott
  • Darcy Mootz
  • Nancy N Huang
  • Erica Farber
  • Yitzhak Pilpel
  • Michael C Fitzgerald
  • Jessica J Smith
  • Julia M Claggett
  • Donna K Slonim
  • Casey Roehrig
  • Andrew A Hill
  • Anandita K Rajpurohit
  • Patrycja E Missiuro
  • Tommi S Jaakkola
  • Hui Ge
  • David K Gifford
  • Ashish Kapoor
  • Sean P Whelan
  • David K Cureton
  • Diana M Ho
  • Marc Vidal
  • Darcy E Mootz
  • Christina Molodowitch
  • Albertha J M Walhout

Detail Information

Publications21

  1. pmc Synthetic lethal analysis of Caenorhabditis elegans posterior embryonic patterning genes identifies conserved genetic interactions
    L Ryan Baugh
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Genome Biol 6:R45. 2005
    ..Genetic interactions within a homologous set of genes involved in vertebrate myogenesis indicate broad conservation of the muscle module and suggest that other genetic modules identified in C. elegans will be conserved...
  2. pmc Composition and regulation of maternal and zygotic transcriptomes reflects species-specific reproductive mode
    Shai S Shen-Orr
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA
    Genome Biol 11:R58. 2010
    ..The differences in maternal contributions between animals may be associated with gene regulatory changes detectable by the size and complexity of the associated regulatory regions...
  3. ncbi request reprint Gene silencing: shrinking the black box of RNAi
    C P Hunter
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Curr Biol 10:R137-40. 2000
    ..Recent results identifying cellular components required for RNAi in Caenorhabditis elegans indicate that the mechanism is conserved, ancient and may provide a defense against selfish DNA...
  4. ncbi request reprint Transport of sequence-specific RNA interference information between cells
    Antony M Jose
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Annu Rev Genet 41:305-30. 2007
    ..elegans. Identifying the transported silencing signal and deciphering the evolutionarily selected role of the transport machinery are some of the key challenges for the future...
  5. ncbi request reprint Transport of dsRNA into cells by the transmembrane protein SID-1
    Evan H Feinberg
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 301:1545-7. 2003
    ..Further analyses revealed that SID-1 enables passive cellular uptake of dsRNA. These data indicate that systemic RNAi in C. elegans involves SID-1-mediated intercellular transport of dsRNA...
  6. doi request reprint Environmental RNA interference
    Jennifer S Whangbo
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Trends Genet 24:297-305. 2008
    ..Here, we describe the characterization and current understanding of environmental RNAi and discuss its potential applications...
  7. pmc Export of RNA silencing from C. elegans tissues does not require the RNA channel SID-1
    Antony M Jose
    Department of Molecular and Cellular Biology, Harvard University, Room 3050, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 106:2283-8. 2009
    ..Thus, C. elegans can transport endogenous and exogenous RNA silencing signals between many different tissues via at least 2 SID-1 independent export pathways...
  8. pmc Pairing of competitive and topologically distinct regulatory modules enhances patterned gene expression
    Itai Yanai
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Syst Biol 4:163. 2008
    ..The coupling of asynchronous and topologically distinct modules may be a general principle of module assembly that functions to potentiate genetic switches...
  9. pmc Caenorhabditis elegans SID-2 is required for environmental RNA interference
    William M Winston
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 104:10565-70. 2007
    ..elegans. SID-2, when expressed in the environmental RNAi defective species Caenorhabditis briggsae, confers environmental RNAi...
  10. pmc Regulators of the actin cytoskeleton mediate lethality in a Caenorhabditis elegans dhc-1 mutant
    Aleksandra J Gil-Krzewska
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Mol Biol Cell 21:2707-20. 2010
    ..These results reveal an unexpected direct or indirect interaction between the actin cytoskeleton and dynein activity...
  11. pmc An antiviral role for the RNA interference machinery in Caenorhabditis elegans
    Daniel H Schott
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Proc Natl Acad Sci U S A 102:18420-4. 2005
    ..Our observation that multiple genes required for RNAi are also required for resistance to vesicular stomatitis virus suggests that the RNAi machinery functions in resistance to viruses in nature...
  12. ncbi request reprint The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo
    L Ryan Baugh
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Development 132:1843-54. 2005
    ..Transcription of targets is initiated in four temporal phases, which together with their spatial expression patterns leads to a model of the regulatory network specified by PAL-1...
  13. ncbi request reprint The STAR/Maxi-KH domain protein GLD-1 mediates a developmental switch in the translational control of C. elegans PAL-1
    Darcy Mootz
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Development 131:3263-72. 2004
    ..Hence, GLD-1 mediates a developmental switch in the control of PAL-1 repression, allowing MEX-3 to accumulate and take over the task of PAL-1 repression in the proximal germline, where GLD-1 protein levels decline...
  14. pmc Mutations in a beta-tubulin disrupt spindle orientation and microtubule dynamics in the early Caenorhabditis elegans embryo
    Amanda J Wright
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Mol Biol Cell 14:4512-25. 2003
    ..tbb-2 is required for centrosome stabilization during anaphase of the first cell division, suggesting that tbb-2 may be specialized for interactions with the cell cortex...
  15. ncbi request reprint Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1
    William M Winston
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
    Science 295:2456-9. 2002
    ..One of these loci, sid-1, encodes a conserved protein with predicted transmembrane domains. SID-1 is expressed in cells sensitive to RNAi, is localized to the cell periphery, and is required cell-autonomously for systemic RNAi...
  16. ncbi request reprint MEX-3 interacting proteins link cell polarity to asymmetric gene expression in Caenorhabditis elegans
    Nancy N Huang
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Development 129:747-59. 2002
    ..We propose that MEX-6 and SPN-4 act with MEX-3 to translate the temporal and spatial information provided by the early acting par genes into the asymmetric expression of the cell fate determinant PAL-1...
  17. pmc Comparison of diverse developmental transcriptomes reveals that coexpression of gene neighbors is not evolutionarily conserved
    Itai Yanai
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genome Res 19:2214-20. 2009
    ..Our results suggest that a significant fraction of the coexpression observed among gene neighbors may be accounted for by neutral processes, and further that these may be distinguished by comparative gene expression analyses...
  18. pmc The SID-1 double-stranded RNA transporter is not selective for dsRNA length
    Joseph D Shih
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    RNA 15:384-90. 2009
    ..Finally, we find that coexpressing mutant SID-1 with wild-type SID-1 in S2 cells interferes with SID-1 function, indicating that SID-1 may function as a multimer...
  19. ncbi request reprint Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome
    L Ryan Baugh
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
    Development 130:889-900. 2003
    ..Finally, a versatile analytical platform based on cluster analysis and developmental classification of genes is provided...
  20. pmc Progression from mitotic catastrophe to germ cell death in Caenorhabditis elegans lis-1 mutants requires the spindle checkpoint
    Edgar A Buttner
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138 2020, USA
    Dev Biol 305:397-410. 2007
    ..These results suggest that apoptosis may contribute to the cell-sparse pathology of lissencephaly...
  21. ncbi request reprint Semi-supervised analysis of gene expression profiles for lineage-specific development in the Caenorhabditis elegans embryo
    Yuan Qi
    Massachusetts Institute of Technology, Computer Science and Artificial Intelligence Laboratory, 32 Vassar Street, Cambridge, MA 02139, USA
    Bioinformatics 22:e417-23. 2006
    ..However, for many biological problems, little prior knowledge is available, which limits the prediction performance of most supervised methods...

Research Grants16

  1. Molecular Genetic Analysis of Extracellular RNAs in C. elegans
    Craig Hunter; Fiscal Year: 2009
    ..Understanding how dsRNA crosses cell membranes to trigger RNAi will lead to improvements in targeting and delivery efficiency, and will limit potential deleterious side effects of this exciting therapeutic strategy. ..
  2. Genetic and Biochemical Analysis of SID-1 and SID-2
    Craig Hunter; Fiscal Year: 2007
    ..Although RNAi has been shown to be effective, a major obstacle remains delivery of dsRNA into human cells both in culture and in vivo. ..
  3. Developmental Genomics in C. elegans
    Craig Hunter; Fiscal Year: 2006
    ..This analysis, although limited to those genes that are transcriptional targets of pal-1 activity, is the necessary first step to a complete description of the activity of a master regulator. ..
  4. TRANSLATIONAL CONTROL IN C. ELEGANS EMBRYOS
    Craig Hunter; Fiscal Year: 2004
    ..abstract_text> ..
  5. Molecular Genetic Analysis of Extracellular RNAs in C. elegans
    CRAIG PATRICK HUNTER; Fiscal Year: 2010
    ..Understanding how dsRNA crosses cell membranes to trigger RNAi will lead to improvements in targeting and delivery efficiency, and will limit potential deleterious side effects of this exciting therapeutic strategy. ..