LAURIE GOODYEAR

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Targeting the AMP-activated protein kinase for the treatment of type 2 diabetes
    Nicolas Musi
    Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Womens s Hospital, Harvard Medical School, Boston, MA, USA
    Curr Drug Targets Immune Endocr Metabol Disord 2:119-27. 2002
  2. ncbi request reprint Akt signaling in skeletal muscle: regulation by exercise and passive stretch
    Kei Sakamoto
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 285:E1081-8. 2003
  3. ncbi request reprint Cloning and characterization of mouse 5'-AMP-activated protein kinase gamma3 subunit
    Haiyan Yu
    Research Division, Joslin Diabetes Center, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 286:C283-92. 2004
  4. pmc Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle
    Ho Jin Koh
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 107:15541-6. 2010
  5. pmc Kinetics of contraction-induced GLUT4 translocation in skeletal muscle fibers from living mice
    Hans P M M Lauritzen
    Research Division, Joslin Diabetes Center and Harvard Medical School, Boston, Massachusetts, USA
    Diabetes 59:2134-44. 2010
  6. pmc Contraction regulates site-specific phosphorylation of TBC1D1 in skeletal muscle
    Kanokwan Vichaiwong
    Research Division, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, U S A
    Biochem J 431:311-20. 2010
  7. pmc Insulin stimulation regulates AS160 and TBC1D1 phosphorylation sites in human skeletal muscle
    R J W Middelbeek
    Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA, USA
    Nutr Diabetes 3:e74. 2013
  8. ncbi request reprint AMP-activated protein kinase: a critical signaling intermediary for exercise-stimulated glucose transport?
    L J Goodyear
    Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
    Exerc Sport Sci Rev 28:113-6. 2000
  9. ncbi request reprint Metformin increases AMP-activated protein kinase activity in skeletal muscle of subjects with type 2 diabetes
    Nicolas Musi
    Research Division, Joslin Diabetes Center, Brigham and Women s Hospital and Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Diabetes 51:2074-81. 2002
  10. ncbi request reprint Targeting skeletal muscle AMP-activated protein kinase to treat type 2 diabetes
    Eric B Taylor
    The Joslin Diabetes Center and Harvard Medical School, 1 Joslin Place, Boston, MA 02215, USA
    Curr Diab Rep 7:399-401. 2007

Detail Information

Publications72

  1. ncbi request reprint Targeting the AMP-activated protein kinase for the treatment of type 2 diabetes
    Nicolas Musi
    Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Womens s Hospital, Harvard Medical School, Boston, MA, USA
    Curr Drug Targets Immune Endocr Metabol Disord 2:119-27. 2002
    ..While strong efforts are underway in order to identify novel AMPK-activating compounds, the safety of chronic pharmacological activation of AMPK remains to be determined...
  2. ncbi request reprint Akt signaling in skeletal muscle: regulation by exercise and passive stretch
    Kei Sakamoto
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 285:E1081-8. 2003
    ..We conclude that physiological exercise in vivo activates Akt in multiple skeletal muscle fiber types and that mechanical tension may be a part of the mechanism by which contraction activates Akt in fast-twitch muscles...
  3. ncbi request reprint Cloning and characterization of mouse 5'-AMP-activated protein kinase gamma3 subunit
    Haiyan Yu
    Research Division, Joslin Diabetes Center, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 286:C283-92. 2004
    ....
  4. pmc Sucrose nonfermenting AMPK-related kinase (SNARK) mediates contraction-stimulated glucose transport in mouse skeletal muscle
    Ho Jin Koh
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Proc Natl Acad Sci U S A 107:15541-6. 2010
    ..SNARK is activated by muscle contraction and is a unique mediator of contraction-stimulated glucose transport in skeletal muscle...
  5. pmc Kinetics of contraction-induced GLUT4 translocation in skeletal muscle fibers from living mice
    Hans P M M Lauritzen
    Research Division, Joslin Diabetes Center and Harvard Medical School, Boston, Massachusetts, USA
    Diabetes 59:2134-44. 2010
    ....
  6. pmc Contraction regulates site-specific phosphorylation of TBC1D1 in skeletal muscle
    Kanokwan Vichaiwong
    Research Division, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, U S A
    Biochem J 431:311-20. 2010
    ..AMPK and Akt regulate TBC1D1 phosphorylation, but there must be additional upstream kinases that mediate TBC1D1 phosphorylation in skeletal muscle...
  7. pmc Insulin stimulation regulates AS160 and TBC1D1 phosphorylation sites in human skeletal muscle
    R J W Middelbeek
    Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Boston, MA, USA
    Nutr Diabetes 3:e74. 2013
    ....
  8. ncbi request reprint AMP-activated protein kinase: a critical signaling intermediary for exercise-stimulated glucose transport?
    L J Goodyear
    Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
    Exerc Sport Sci Rev 28:113-6. 2000
    ..This brief report will discuss an emerging hypothesis that suggests that the AMP-activated protein kinase is a key signaling intermediary leading to exercise-stimulated GLUT4 translocation in skeletal muscle...
  9. ncbi request reprint Metformin increases AMP-activated protein kinase activity in skeletal muscle of subjects with type 2 diabetes
    Nicolas Musi
    Research Division, Joslin Diabetes Center, Brigham and Women s Hospital and Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Diabetes 51:2074-81. 2002
    ..These findings suggest that the metabolic effects of metformin in subjects with type 2 diabetes may be mediated by the activation of AMPK alpha2...
  10. ncbi request reprint Targeting skeletal muscle AMP-activated protein kinase to treat type 2 diabetes
    Eric B Taylor
    The Joslin Diabetes Center and Harvard Medical School, 1 Joslin Place, Boston, MA 02215, USA
    Curr Diab Rep 7:399-401. 2007
  11. ncbi request reprint Muscle-specific overexpression of wild type and R225Q mutant AMP-activated protein kinase gamma3-subunit differentially regulates glycogen accumulation
    Haiyan Yu
    Joslin Diabetes Center, Boston, MA 02215, USA
    Am J Physiol Endocrinol Metab 291:E557-65. 2006
    ..Furthermore, mutation of the gamma3-subunit is associated with decreases in AMPKalpha2 isoform-specific activity and impairment in AICAR- and phenformin-stimulated skeletal muscle glucose uptake...
  12. pmc Dissociation of AMP-activated protein kinase and p38 mitogen-activated protein kinase signaling in skeletal muscle
    Richard C Ho
    Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
    Biochem Biophys Res Commun 362:354-9. 2007
    ..Furthermore, AMPKalpha2i TG mice lacking contraction-stimulated AMPK activity have normal p38 MAPK phosphorylation. These results suggest that p38 MAPK is not a downstream component of AMPK-mediated signaling in skeletal muscle...
  13. ncbi request reprint AMP-activated protein kinase alpha2 activity is not essential for contraction- and hyperosmolarity-induced glucose transport in skeletal muscle
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 280:39033-41. 2005
    ..Muscle contraction- and hyperosmolarity-induced glucose transport may be regulated by a redundant mechanism in which AMPK alpha2 is one of multiple signaling pathways...
  14. ncbi request reprint Effect of AICAR treatment on glycogen metabolism in skeletal muscle
    William G Aschenbach
    Research Division, Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 51:567-73. 2002
    ..Rather, the increased glycogen is due to the well-known effects of AICAR to increase glucose uptake...
  15. pmc Discovery of TBC1D1 as an insulin-, AICAR-, and contraction-stimulated signaling nexus in mouse skeletal muscle
    Eric B Taylor
    The Joslin Diabetes Center Section on Metabolism and Proteomics Core and Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    J Biol Chem 283:9787-96. 2008
    ..TBC1D1 is a major PAS immunoreactive protein in skeletal muscle that is phosphorylated in vivo by insulin, AICAR, and contraction. Both Akt and AMPK phosphorylate TBC1D1, but AMPK may be the more robust regulator...
  16. pmc Ablation of AMP-activated protein kinase alpha2 activity exacerbates insulin resistance induced by high-fat feeding of mice
    Nobuharu Fujii
    Department of Medicine, Research Division, Joslin Diabetes Center, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    Diabetes 57:2958-66. 2008
    ..We determined whether muscle AMP-activated protein kinase (AMPK) has a role in the development of insulin resistance...
  17. pmc Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle
    Jonas T Treebak
    Joslin Diabetes Center, Section on Metabolism, Harvard Medical School, Boston, MA 02215, USA
    Am J Physiol Cell Physiol 298:C377-85. 2010
    ..S711 is a novel TBC1D4 phosphorylation site regulated by AMPK in skeletal muscle...
  18. ncbi request reprint Loss of class IA PI3K signaling in muscle leads to impaired muscle growth, insulin response, and hyperlipidemia
    Ji Luo
    Department of Systems Biology, Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA
    Cell Metab 3:355-66. 2006
    ..Our finding also indicates that compromised muscle PI3K signaling could contribute to symptoms of hyperlipidemia associated with human type 2 diabetes...
  19. ncbi request reprint Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle
    Henning F Kramer
    Section Head, Metabolism, Joslin Diabetes Center, One Joslin Pl, Boston, MA 02215, USA
    Diabetes 55:2067-76. 2006
    ..While Akt and AMPK alpha2 activities are essential for AS160 phosphorylation by insulin and AICAR, respectively, neither kinase is indispensable for the entire effects of contraction on AS160 phosphorylation...
  20. ncbi request reprint Calmodulin-binding domain of AS160 regulates contraction- but not insulin-stimulated glucose uptake in skeletal muscle
    Henning F Kramer
    Department of Metabolism, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Diabetes 56:2854-62. 2007
    ..This raises the possibility that contraction-stimulated increases in Ca(2+)/calmodulin could also modulate AS160 function...
  21. ncbi request reprint The exercise pill--too good to be true?
    Laurie J Goodyear
    Joslin Diabetes Center and Harvard Medical School, Boston, USA
    N Engl J Med 359:1842-4. 2008
  22. pmc Muscle-specific PPARgamma-deficient mice develop increased adiposity and insulin resistance but respond to thiazolidinediones
    Andrew W Norris
    Research Division, Joslin Diabetes Center, One Joslin Place, Boston, Massachusetts 02215, USA
    J Clin Invest 112:608-18. 2003
    ..The tissue crosstalk mediating these effects is perhaps due to altered lipid metabolism in muscle...
  23. ncbi request reprint Role of Akt2 in contraction-stimulated cell signaling and glucose uptake in skeletal muscle
    Kei Sakamoto
    The Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 291:E1031-7. 2006
    ....
  24. ncbi request reprint Increased alpha2 subunit-associated AMPK activity and PRKAG2 cardiomyopathy
    Ferhaan Ahmad
    Department of Genetics, Harvard Medical School, Howard Hughes Medical Institute, Boston, MA, USA
    Circulation 112:3140-8. 2005
    ..Mice overexpressing a dominant-negative alpha2 subunit of AMPK (TGalpha2DN) provide a tool for selectively inhibiting alpha2, but not alpha1, subunit-associated AMPK activity...
  25. ncbi request reprint Skeletal muscle adaptation to exercise training: AMP-activated protein kinase mediates muscle fiber type shift
    Katja S C Röckl
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital, and Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 56:2062-9. 2007
    ..AMPK is not necessary for exercise training-induced increases in mitochondrial markers, but it is essential for fiber type IIb to IIa/x transformation and increases in hexokinase II protein...
  26. pmc LKB1 and AMPK and the regulation of skeletal muscle metabolism
    Ho Jin Koh
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Curr Opin Clin Nutr Metab Care 11:227-32. 2008
    ..To address the role of LKB1 and AMP-activated protein kinase (AMPK) in glucose transport, fatty acid oxidation, and metabolic adaptations in skeletal muscle...
  27. ncbi request reprint Ca2+/calmodulin-dependent protein kinase kinase-alpha regulates skeletal muscle glucose uptake independent of AMP-activated protein kinase and Akt activation
    Carol A Witczak
    Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
    Diabetes 56:1403-9. 2007
    ..Collectively, these results implicate CaMKKalpha in the regulation of skeletal muscle glucose uptake independent of AMPK and Akt activation...
  28. ncbi request reprint Regulation of dishevelled and beta-catenin in rat skeletal muscle: an alternative exercise-induced GSK-3beta signaling pathway
    William G Aschenbach
    Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Am J Physiol Endocrinol Metab 291:E152-8. 2006
    ..In conclusion, exercise in vivo, but not insulin, increases the association between Dvl and GSK-3beta in skeletal muscle, an event paralleled by beta-catenin dephosphorylation...
  29. ncbi request reprint Role of AMP-activated protein kinase in exercise capacity, whole body glucose homeostasis, and glucose transport in skeletal muscle -insight from analysis of a transgenic mouse model-
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, and the Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, One Joslin Place, Boston, MA 02215, USA
    Diabetes Res Clin Pract 77:S92-8. 2007
    ..However, contraction- or hyperosmolarity-induced glucose transport was nearly normal. These results suggest that AMPK alpha2 activation is essential for some, but not all insulin-independent glucose transport...
  30. ncbi request reprint Invited review: intracellular signaling in contracting skeletal muscle
    Kei Sakamoto
    Research Division, Joslin Diabetes Center, Boston, Massachusetts 02215, USA
    J Appl Physiol 93:369-83. 2002
    ..A fundamental goal of signaling research is to determine the biological consequences of exercise-induced signaling through these molecules, and this review also provides an update of progress in this area...
  31. ncbi request reprint Large GLUT4 vesicles are stationary while locally and reversibly depleted during transient insulin stimulation of skeletal muscle of living mice: imaging analysis of GLUT4-enhanced green fluorescent protein vesicle dynamics
    Hans P M M Lauritzen
    Research Division, Joslin Diabetes Center and Harvard Medical School, Boston, MA 02215, USA
    Diabetes 57:315-24. 2008
    ..We studied in living animals the recruitment of GLUT4 vesicles in more detail than previously done and, for the first time, analyzed the steady-state recycling and subsequent re-internalization of GLUT4 on an insulin bolus...
  32. ncbi request reprint Exercise induces hypoglycemia in rats with islet transplantation
    Abdulkadir Omer
    Section on Islet Transplantation and Cell Biology, Joslin Diabetes Center, Boston, Massachusetts, USA
    Diabetes 53:360-5. 2004
    ..C-peptide and glucagon responses to exercise were very different in rats with transplanted islets compared with controls. This islet dysfunction led to exercise-induced hypoglycemia...
  33. ncbi request reprint Glucose metabolism and energy homeostasis in mouse hearts overexpressing dominant negative alpha2 subunit of AMP-activated protein kinase
    Yanqiu Xing
    Nuclear Magnetic Resonance Laboratory for Physiological Chemistry, Cardiovascular Division and Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:28372-7. 2003
    ..Thus, AMPK alpha2 is necessary for maintaining myocardial energy homeostasis during ischemia. It is likely that the functional role of AMPK in myocardial energy metabolism resides both in energy supply and utilization...
  34. ncbi request reprint p38gamma MAPK regulation of glucose transporter expression and glucose uptake in L6 myotubes and mouse skeletal muscle
    Richard C Ho
    Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    Am J Physiol Regul Integr Comp Physiol 286:R342-9. 2004
    ..These findings are consistent with the hypothesis that activation of stress kinases such as p38 are negative regulators of stimulated glucose uptake in peripheral tissues...
  35. pmc CaMKII regulates contraction- but not insulin-induced glucose uptake in mouse skeletal muscle
    Carol A Witczak
    Joslin Diabetes Center, Research Division, Department of Medicine, Brigham and Women sHospital, and Harvard Medical School, Boston, MA, 02215, USA
    Am J Physiol Endocrinol Metab 298:E1150-60. 2010
    ..These results demonstrate that CaMKII does not regulate insulin-stimulated glucose uptake in skeletal muscle. However, CaMKII plays a critical role in the regulation of contraction-induced glucose uptake in mouse skeletal muscle...
  36. pmc Reduced expression of the murine p85alpha subunit of phosphoinositide 3-kinase improves insulin signaling and ameliorates diabetes
    Franck Mauvais-Jarvis
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Invest 109:141-9. 2002
    ..Furthermore, Pik3r1 heterozygosity protects mice with genetic insulin resistance from developing diabetes. These data suggest that regulation of p85alpha levels may provide a novel therapeutic target for the treatment of type 2 diabetes...
  37. pmc Aberrant activation of AMP-activated protein kinase remodels metabolic network in favor of cardiac glycogen storage
    Ivan Luptak
    NMR Laboratory for Physiological Chemistry, Division of Cardiovascular Medicine, Brigham and Women s Hospital and Harvard Medical School, 221 Longwood Avenue, Boston, MA 02115, USA
    J Clin Invest 117:1432-9. 2007
    ..These findings are of particular importance in considering AMPK as a target for the treatment of metabolic diseases...
  38. pmc TBC1D1 regulates insulin- and contraction-induced glucose transport in mouse skeletal muscle
    Ding An
    Research Division, Joslin Diabetes Center, Boston, Massachusetts, USA
    Diabetes 59:1358-65. 2010
    ..Genetic linkage analyses show a TBC1D1 R125W missense variant confers risk for severe obesity in humans. The objective of this study was to determine whether TBC1D1 regulates glucose transport in skeletal muscle...
  39. ncbi request reprint Exercise regulates Akt and glycogen synthase kinase-3 activities in human skeletal muscle
    Kei Sakamoto
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
    Biochem Biophys Res Commun 319:419-25. 2004
    ....
  40. ncbi request reprint Regulation of glucose transport by the AMP-activated protein kinase
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02215, USA
    Proc Nutr Soc 63:205-10. 2004
    ..Thus, during the next several years considerably more research will be necessary in order to fully understand the role of AMPK in regulating glucose transport in skeletal muscle...
  41. ncbi request reprint Beta-cell secretory dysfunction in the pathogenesis of low birth weight-associated diabetes: a murine model
    Josep C Jimenez-Chillaron
    Research Division, Joslin Diabetes Center, Boston, Massachusetts, USA
    Diabetes 54:702-11. 2005
    ..5-fold increase in hexokinase activity. Taken together, these data suggest that, at least in mice, primary beta-cell dysfunction may play a significant role in the pathogenesis of LBW-associated type 2 diabetes...
  42. ncbi request reprint Contraction signaling to glucose transport in skeletal muscle
    Niels Jessen
    Research Div, Joslin Diabetes Center, and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02215, USA
    J Appl Physiol 99:330-7. 2005
    ..Further research is needed because full elucidation of an insulin-independent signal leading to glucose transport would be a promising pharmacological target for the treatment of Type 2 diabetes...
  43. ncbi request reprint Regulation of IkappaB kinase and NF-kappaB in contracting adult rat skeletal muscle
    Richard C Ho
    Research Division, Joslin Diabetes Center, 1 Joslin Pl, Boston, Massachusetts 02215, USA
    Am J Physiol Cell Physiol 289:C794-801. 2005
    ..In conclusion, acute submaximal exercise transiently stimulates NF-kappaB signaling in skeletal muscle. This activation is a local event because it can occur in the absence of exercise-derived systemic factors...
  44. ncbi request reprint Insulin signaling after exercise in insulin receptor substrate-2-deficient mice
    Kirsten F Howlett
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    Diabetes 51:479-83. 2002
    ..IRS-2 signaling can partially account for the increase in insulin-stimulated phosphotyrosine-associated PI 3-kinase activity after exercise...
  45. pmc Signaling mechanisms in skeletal muscle: acute responses and chronic adaptations to exercise
    Katja S C Röckl
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA, USA
    IUBMB Life 60:145-53. 2008
    ....
  46. pmc Effects of exercise training on subcutaneous and visceral adipose tissue in normal- and high-fat diet-fed rats
    Katja S C Gollisch
    Research Division, Department of Medicine, and Harvard Medical School, Joslin Diabetes Center, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 297:E495-504. 2009
    ..Improved glucose tolerance was accompanied by an increase in cytokine gene expression in subcutaneous fat. This finding raises the possibility of a specific role of subcutaneous adipose tissue in adaptive responses to exercise training...
  47. ncbi request reprint Contraction regulation of Akt in rat skeletal muscle
    Kei Sakamoto
    Research Division, Joslin Diabetes Center and the Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 277:11910-7. 2002
    ..Akt signaling is not necessary for the regulation of glycogen synthase activity in contracting skeletal muscle...
  48. ncbi request reprint cDNA cloning and functional characterization of a novel splice variant of c-Cbl-associated protein from mouse skeletal muscle
    Oscar Alcazar
    The Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, MA 02215, USA
    Biochem Biophys Res Commun 317:285-93. 2004
    ..These data demonstrate the existence of a novel CAP isoform expressed in skeletal muscle, and suggest the involvement of the CAP/Cbl pathway in the regulation of insulin-stimulated glucose uptake in L6 myotubes...
  49. pmc Skeletal muscle-selective knockout of LKB1 increases insulin sensitivity, improves glucose homeostasis, and decreases TRB3
    Ho Jin Koh
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Mol Cell Biol 26:8217-27. 2006
    ..LKB1-mediated TRB3 expression provides a novel link between LKB1 and Akt, critical kinases involved in both tumor genesis and cell metabolism...
  50. ncbi request reprint Functional role of AMP-activated protein kinase in the heart during exercise
    Nicolas Musi
    Research Division, Joslin Diabetes Center, One Joslin Place, Boston, MA 02215, USA
    FEBS Lett 579:2045-50. 2005
    ..We found that inactivation of AMPK did not result in abnormal ATP and glycogen consumption during exercise, cardiac function assessed by heart rhythm telemetry and stress echocardiography, or in maximal exercise capacity...
  51. ncbi request reprint AMP-activated protein kinase and the regulation of glucose transport
    Nobuharu Fujii
    Research Division, Joslin Diabetes Center and Department of Medicine, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Endocrinol Metab 291:E867-77. 2006
    ..The identification of AMPK as a novel glucose transport mediator in skeletal muscle is providing important insights for the treatment and prevention of type 2 diabetes...
  52. ncbi request reprint 5' adenosine monophosphate-activated protein kinase, metabolism and exercise
    William G Aschenbach
    Research Division, Joslin Diabetes Center and Harvard Medical School, Boston, Massachusetts, USA
    Sports Med 34:91-103. 2004
    ..This review discusses the putative roles of AMPK in acute and chronic exercise responses, and suggests avenues for future AMPK research in exercise physiology and biochemistry...
  53. ncbi request reprint Overexpression or ablation of JNK in skeletal muscle has no effect on glycogen synthase activity
    Nobuharu Fujii
    Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA
    Am J Physiol Cell Physiol 287:C200-8. 2004
    ..JNK activation is unlikely to be the major mechanism by which contractile activity increases glycogen synthase activity in skeletal muscle...
  54. pmc Muscle-specific deletion of the Glut4 glucose transporter alters multiple regulatory steps in glycogen metabolism
    Young Bum Kim
    Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, 99 Brookline Avenue, Boston, MA 02215, USA
    Mol Cell Biol 25:9713-23. 2005
    ..This, combined with decreased glycogen phosphorylase activity, results in increased glycogen content in muscle in the fasted state when glucose transport is reduced...
  55. pmc Highly efficient, functional engraftment of skeletal muscle stem cells in dystrophic muscles
    Massimiliano Cerletti
    Section on Developmental and Stem Cell Biology, Joslin Diabetes Center, One Joslin Place, Boston, MA 02115, USA
    Cell 134:37-47. 2008
    ..Together, these studies indicate the presence in adult skeletal muscle of prospectively isolatable muscle-forming stem cells and directly demonstrate the efficacy of myogenic stem cell transplant for treating muscle degenerative disease...
  56. ncbi request reprint Exercise, MAPK, and NF-kappaB signaling in skeletal muscle
    Henning F Kramer
    Metabolism, Research Division, Joslin Diabetes Center, Brigham and Women s Hospital and Harvard Medical School, Boston, USA
    J Appl Physiol 103:388-95. 2007
    ..The objectives herein are twofold: 1) to determine how and why exercise activates MAPK and NF-kappaB; and 2) to resolve their paradoxical activation during diseased and healthy conditions...
  57. pmc Adrenaline is a critical mediator of acute exercise-induced AMP-activated protein kinase activation in adipocytes
    Ho Jin Koh
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
    Biochem J 403:473-81. 2007
    ..Our findings suggest that adrenaline plays a critical role in exercise-stimulated AMPKalpha1 and alpha2 activities in adipocytes, and that AMPK can function in the regulation of lipolysis...
  58. pmc Estradiol stimulates Akt, AMP-activated protein kinase (AMPK) and TBC1D1/4, but not glucose uptake in rat soleus
    Nicole H Rogers
    Obesity and Metabolism, JM USDA HNRCA at Tufts University, Boston, MA 02111, USA
    Biochem Biophys Res Commun 382:646-50. 2009
    ..This study is the first to demonstrate that estradiol stimulates Akt, AMPK, and TBC1D1/4 in intact skeletal muscle, but surprisingly, estradiol does not stimulate muscle glucose uptake...
  59. ncbi request reprint Insulin resistance and lipodystrophy in mice lacking ribosomal S6 kinase 2
    Karim El-Haschimi
    Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215, USA
    Diabetes 52:1340-6. 2003
    ....
  60. pmc Exercise increases TBC1D1 phosphorylation in human skeletal muscle
    Niels Jessen
    Joslin Diabetes Center, Boston, MA 02215, USA
    Am J Physiol Endocrinol Metab 301:E164-71. 2011
    ..These data demonstrate that a single bout of exercise regulates TBC1D1 and AS160 phosphorylation on multiple sites in human skeletal muscle...
  61. pmc p50alpha/p55alpha phosphoinositide 3-kinase knockout mice exhibit enhanced insulin sensitivity
    Dong Chen
    Research Division, Joslin Diabetes Center and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02215, USA
    Mol Cell Biol 24:320-9. 2004
    ..Taken together, these data indicate that p50alpha and p55alpha play an important role in insulin signaling and action, especially in lipid and glucose metabolism...
  62. pmc Ablation of LKB1 in the heart leads to energy deprivation and impaired cardiac function
    Niels Jessen
    Joslin Diabetes Center, Boston, MA 02215, USA
    Biochim Biophys Acta 1802:593-600. 2010
    ..Ablation of LKB1 leads to a decreased cardiac efficiency despite normal mitochondrial oxidative metabolism...
  63. ncbi request reprint AMP-activated protein kinase activates transcription of the UCP3 and HKII genes in rat skeletal muscle
    James Stoppani
    The John B Pierce Laboratory and Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut 06519, USA
    Am J Physiol Endocrinol Metab 283:E1239-48. 2002
    ..These data provide evidence that AMPK signaling is linked to the transcriptional regulation of select metabolic genes in skeletal muscle...
  64. ncbi request reprint Genetic model for the chronic activation of skeletal muscle AMP-activated protein kinase leads to glycogen accumulation
    Laura Barré
    Department of Medicine, Dartmouth Medical School, Dartmouth College, Hanover, NH 03755 3833, USA
    Am J Physiol Endocrinol Metab 292:E802-11. 2007
    ..Transgene expression of this mutant AMPKgamma1 subunit may provide a useful model for the chronic activation of AMPK in other tissues to clarify its multiple roles in the regulation of metabolism and other physiological processes...
  65. ncbi request reprint Long-term AICAR administration and exercise prevents diabetes in ZDF rats
    Rasmus Pold
    Medical Research Laboratory and Medical Department M Endocrinology and Diabetes, Aarhus University Hospital, Aarhus, Denmark
    Diabetes 54:928-34. 2005
    ..Our results suggest that activation of AMPK may represent a therapeutic approach to improve insulin action and prevent a decrease in beta-cell function associated with type 2 diabetes...
  66. ncbi request reprint AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse skeletal muscle
    Henning F Kramer
    Joslin Diabetes Center Research Division, Metabolism Section, Massachusetts 02215, USA
    J Biol Chem 281:31478-85. 2006
    ..Our findings directly implicate AS160 as a critical convergence factor for independent stimulators of skeletal muscle glucose uptake...
  67. ncbi request reprint 5'-AMP-activated protein kinase activity and subunit expression in exercise-trained human skeletal muscle
    Jakob N Nielsen
    Institute of Exercise and Sport Sciences, Copenhagen Muscle Research Centre, University of Copenhagen, Denmark
    J Appl Physiol (1985) 94:631-41. 2003
    ..01). In conclusion, trained human skeletal muscle has increased alpha(1)-AMPK protein levels and blunted AMPK activation during exercise...
  68. ncbi request reprint Insulin promotes glycogen synthesis in the absence of GSK3 phosphorylation in skeletal muscle
    Michale Bouskila
    Am J Physiol Endocrinol Metab 294:E28-35. 2008
    ..This suggests that allosteric regulation of GS by G-6-P may play a key role in insulin-stimulated muscle glycogen synthesis in vivo...
  69. ncbi request reprint Insulin-stimulated insulin receptor substrate-2-associated phosphatidylinositol 3-kinase activity is enhanced in human skeletal muscle after exercise
    Kirsten F Howlett
    Center for Physical Activity and Nutrition C PAN, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria 3125, Australia
    Metabolism 55:1046-52. 2006
    ..In conclusion, in the immediate period after an acute bout of exercise, insulin-stimulated IRS-2 signaling is enhanced in human skeletal muscle...
  70. ncbi request reprint Insulin resistance and improvements in signal transduction
    Nicolas Musi
    Texas Diabetes Institute and University of Texas Health Science Center at San Antonio, USA
    Endocrine 29:73-80. 2006
    ..The effect of AMPK to stimulate muscle glucose disposal and to control appetite makes it an important pharmacologic target for the treatment of type 2 diabetes and obesity...
  71. ncbi request reprint Exercise training decreases the concentration of malonyl-CoA and increases the expression and activity of malonyl-CoA decarboxylase in human muscle
    Jeanette E Kuhl
    Dept of Molecular Medicine and Surgery, Karolinska Institutet, S 171 76 Stockholm, Sweden
    Am J Physiol Endocrinol Metab 290:E1296-303. 2006
    ..These changes persist after the increases in AMPK activity and whole body insulin sensitivity and fatty acid oxidation, typically caused by an acute bout of exercise in healthy individuals, have dissipated...
  72. pmc Oxidative stress stimulates skeletal muscle glucose uptake through a phosphatidylinositol 3-kinase-dependent pathway
    Yasuki Higaki
    Department of Preventive Medicine, Faculty of Medicine, Saga University, Saga, Japan
    Am J Physiol Endocrinol Metab 294:E889-97. 2008
    ..These results demonstrate that acute exposure of muscle to ROS is a potent stimulator of skeletal muscle glucose uptake and that this occurs through a PI3K-dependent mechanism...

Research Grants36

  1. Regulation of Serine/Threonine Kinases in Skeletal Muscle
    Laurie J Goodyear; Fiscal Year: 2010
    ..This will lead to a better understanding of the beneficial effects of exercise in diabetes and could help to identify novel therapies for diabetes. ..
  2. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2003
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  3. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 2003
    ..Ultimately, these studies should provide us with a better understanding of how regular physical exercise exerts salutary effects on several human diseases. ..
  4. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2002
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  5. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2002
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  6. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 2005
    ..Ultimately, these studies should provide us with a better understanding of how regular physical exercise exerts salutary effects on several human diseases. ..
  7. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 2006
    ..Ultimately, these studies should provide us with a better understanding of how regular physical exercise exerts salutary effects on several human diseases. ..
  8. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2006
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  9. Regultion of Serine/Threonine Kinases in Skeletal Muscle
    LAURIE GOODYEAR; Fiscal Year: 2007
    ..This work should lead to a better understanding of the molecular signaling mechanisms responsible for the beneficial effects of physical exercise in skeletal muscle. ..
  10. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2007
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  11. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 2009
    ..The goal of this proposal is to determine the molecular basis for these beneficial effects in exercising muscles, which may then be used to develop breakthrough drugs designed to treat diabetes. ..
  12. Regulation of Serine/Threonine Kinases in Skeletal Muscle
    LAURIE GOODYEAR; Fiscal Year: 2009
    ..This will lead to a better understanding of the beneficial effects of exercise in diabetes and could help to identify novel therapies for diabetes. ..
  13. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    Laurie J Goodyear; Fiscal Year: 2010
    ..The goal of this proposal is to determine the molecular basis for these beneficial effects in exercising muscles, which may then be used to develop breakthrough drugs designed to treat diabetes. ..
  14. Exercise Regulation of Glucose Metabolism in Skeletal Muscle
    Laurie J Goodyear; Fiscal Year: 2010
    ..g. LKB1, SNARK, TRB3) that are likely to be important in mediating the beneficial effects of exercise and their elucidation could lead to the development of novel therapeutic options for individuals with type 2 diabetes. ..
  15. RNAi Technology to Study In Vivo Muscle Metabolism
    LAURIE GOODYEAR; Fiscal Year: 2005
    ..Ultimately, this approach could provide the basis for treatment of skeletal muscle insulin resistance and other chronic muscle diseases such as the dystrophies. ..
  16. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2004
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  17. Regulation of Serine/Threonine Kinase in Skeletal Muscle
    LAURIE GOODYEAR; Fiscal Year: 2004
    ..This work should lead to a better understanding of the molecular signaling mechanisms responsible for the beneficial effects of physical exercise in skeletal muscle. ..
  18. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 2001
    ..Ultimately, these studies should provide us with a better understanding of how regular physical exercise exerts salutary effects on human diseases. ..
  19. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2001
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  20. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 2002
    ..Ultimately, these studies should provide us with a better understanding of how regular physical exercise exerts salutary effects on several human diseases. ..
  21. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 2004
    ..Ultimately, these studies should provide us with a better understanding of how regular physical exercise exerts salutary effects on several human diseases. ..
  22. Regultion of Serine/Threonine Kinases in Skeletal Muscle
    LAURIE GOODYEAR; Fiscal Year: 2006
    ..This work should lead to a better understanding of the molecular signaling mechanisms responsible for the beneficial effects of physical exercise in skeletal muscle. ..
  23. Regultion of Serine/Threonine Kinases in Skeletal Muscle
    LAURIE GOODYEAR; Fiscal Year: 2005
    ..This work should lead to a better understanding of the molecular signaling mechanisms responsible for the beneficial effects of physical exercise in skeletal muscle. ..
  24. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 1999
    ..Ultimately, these studies should provide us with a better understanding of how regular physical exercise exerts salutary effects on human diseases. ..
  25. Exercise Regulation of Glucose Metabolism in Skeletal Muscle
    LAURIE GOODYEAR; Fiscal Year: 2009
    ..g. LKB1, SNARK, TRB3) that are likely to be important in mediating the beneficial effects of exercise and their elucidation could lead to the development of novel therapeutic options for individuals with type 2 diabetes. ..
  26. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 1999
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  27. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2000
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  28. EXERCISE AND SKELETAL MUSCLE SIGNALING MECHANISMS
    LAURIE GOODYEAR; Fiscal Year: 2000
    ..Ultimately, these studies should provide us with a better understanding of how regular physical exercise exerts salutary effects on human diseases. ..
  29. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2005
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..
  30. AMPK: Impact on Metabolism and Disease
    LAURIE GOODYEAR; Fiscal Year: 2006
    ....
  31. EXERCISE REGULATION OF GLUCOSE UPTAKE IN SKELETAL MUSCLE
    LAURIE GOODYEAR; Fiscal Year: 2002
    ..Ultimately, these studies should provide us with a better understanding of glucose regulation during exercise in healthy people and in individuals with diabetes. ..