Raina Fichorova

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Biocompatibility of solid-dosage forms of anti-human immunodeficiency virus type 1 microbicides with the human cervicovaginal mucosa modeled ex vivo
    Radiana T Trifonova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, 221 Longwood Avenue, Boston, MA 02115, USA
    Antimicrob Agents Chemother 50:4005-10. 2006
  2. pmc The villain team-up or how Trichomonas vaginalis and bacterial vaginosis alter innate immunity in concert
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Sex Transm Infect 89:460-6. 2013
  3. pmc Homeostatic properties of Lactobacillus jensenii engineered as a live vaginal anti-HIV microbicide
    Hidemi S Yamamoto
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    BMC Microbiol 13:4. 2013
  4. pmc Endobiont viruses sensed by the human host - beyond conventional antiparasitic therapy
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School and Brigham and Women s Hospital, Boston, MA, USA
    PLoS ONE 7:e48418. 2012
  5. pmc Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study
    Raina N Fichorova
    Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Anal Chem 80:4741-51. 2008
  6. pmc Polyanionic microbicides modify Toll-like receptor-mediated cervicovaginal immune responses
    R T Trifonova
    Department of Obstetrics, Brigham and Women s Hospital, Laboratory of Genital Tract Biology, Boston, MA 02115, USA
    Antimicrob Agents Chemother 53:1490-500. 2009
  7. pmc Impact of T. vaginalis infection on innate immune responses and reproductive outcome
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02155, USA
    J Reprod Immunol 83:185-9. 2009
  8. doi request reprint Baseline variation and associations between subject characteristics and five cytokine biomarkers of vaginal safety among healthy non-pregnant women in microbicide trials
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Cytokine 55:134-40. 2011
  9. pmc Novel vaginal microflora colonization model providing new insight into microbicide mechanism of action
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Boston, Massachusetts, USA
    MBio 2:e00168-11. 2011
  10. ncbi request reprint Differential expression of immunobiological mediators by immortalized human cervical and vaginal epithelial cells
    R N Fichorova
    Fearing Research Laboratory, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biol Reprod 60:508-14. 1999

Detail Information

Publications27

  1. pmc Biocompatibility of solid-dosage forms of anti-human immunodeficiency virus type 1 microbicides with the human cervicovaginal mucosa modeled ex vivo
    Radiana T Trifonova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, 221 Longwood Avenue, Boston, MA 02115, USA
    Antimicrob Agents Chemother 50:4005-10. 2006
    ..This study identifies immunoinflammatory biomarkers that can discriminate between formulations better than toxicity assays and should be clinically validated in relevance to the risk of HIV-1 acquisition...
  2. pmc The villain team-up or how Trichomonas vaginalis and bacterial vaginosis alter innate immunity in concert
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Sex Transm Infect 89:460-6. 2013
    ....
  3. pmc Homeostatic properties of Lactobacillus jensenii engineered as a live vaginal anti-HIV microbicide
    Hidemi S Yamamoto
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    BMC Microbiol 13:4. 2013
    ....
  4. pmc Endobiont viruses sensed by the human host - beyond conventional antiparasitic therapy
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School and Brigham and Women s Hospital, Boston, MA, USA
    PLoS ONE 7:e48418. 2012
    ..Metronidazole treatment amplified these proinflammatory responses. Thus, a new paradigm targeting the protozoan viruses along with the protozoan host may prevent trichomoniasis-attributable inflammatory sequelae...
  5. pmc Biological and technical variables affecting immunoassay recovery of cytokines from human serum and simulated vaginal fluid: a multicenter study
    Raina N Fichorova
    Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Anal Chem 80:4741-51. 2008
    ..This investigation provides the first standardized analytic approach for assessing differences in mucosal cytokine levels and may improve strategies for monitoring immune responses at the vaginal mucosal interface...
  6. pmc Polyanionic microbicides modify Toll-like receptor-mediated cervicovaginal immune responses
    R T Trifonova
    Department of Obstetrics, Brigham and Women s Hospital, Laboratory of Genital Tract Biology, Boston, MA 02115, USA
    Antimicrob Agents Chemother 53:1490-500. 2009
    ..Further studies are warranted to determine the impact of this interference on HIV-1 transmission risk...
  7. pmc Impact of T. vaginalis infection on innate immune responses and reproductive outcome
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02155, USA
    J Reprod Immunol 83:185-9. 2009
    ..The molecular mechanisms underlying reproductive complications and epidemiologic risks associated with T. vaginalis infection remain to be elucidated...
  8. doi request reprint Baseline variation and associations between subject characteristics and five cytokine biomarkers of vaginal safety among healthy non-pregnant women in microbicide trials
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Cytokine 55:134-40. 2011
    ....
  9. pmc Novel vaginal microflora colonization model providing new insight into microbicide mechanism of action
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Boston, Massachusetts, USA
    MBio 2:e00168-11. 2011
    ....
  10. ncbi request reprint Differential expression of immunobiological mediators by immortalized human cervical and vaginal epithelial cells
    R N Fichorova
    Fearing Research Laboratory, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biol Reprod 60:508-14. 1999
    ....
  11. ncbi request reprint The molecular basis of nonoxynol-9-induced vaginal inflammation and its possible relevance to human immunodeficiency virus type 1 transmission
    R N Fichorova
    Fearing Research Laboratory, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, 75 Francis St, Thorn 217, Boston, MA 02115, USA
    J Infect Dis 184:418-28. 2001
    ..Furthermore, this study identifies in vitro and in vivo model systems for monitoring undesirable proinflammatory effects of microbicides and other vaginal products...
  12. ncbi request reprint Interleukin (IL)-1, IL-6, and IL-8 predict mucosal toxicity of vaginal microbicidal contraceptives
    R N Fichorova
    Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biol Reprod 71:761-9. 2004
    ..The described evaluation system is a valuable tool in identifying novel vaginal contraceptive microbicides, selecting out candidates that may enhance, rather than decrease, HIV transmission...
  13. pmc Anti-human immunodeficiency virus type 1 microbicide cellulose acetate 1,2-benzenedicarboxylate in a human in vitro model of vaginal inflammation
    R N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women s Hospital, 221 Longwood Ave RF468, Boston, MA 02115, USA
    Antimicrob Agents Chemother 49:323-35. 2005
    ..The described system may be useful for predicting proinflammatory side effects of other microbicide candidates for vaginal application...
  14. doi request reprint The relationship between early concentrations of 25 blood proteins and cerebral white matter injury in preterm newborns: the ELGAN study
    Alan Leviton
    Neuroepidemiology Unit, Department of Neurology, Children s Hospital Boston, Harvard Medical School, Boston, MA, USA
    J Pediatr 158:897-903.e1-5. 2011
    ..To evaluate whether concentrations of inflammation-related proteins are elevated in the blood of preterm newborns who develop cerebral white matter damage...
  15. pmc Distinct proinflammatory host responses to Neisseria gonorrhoeae infection in immortalized human cervical and vaginal epithelial cells
    R N Fichorova
    Fearing Research Laboratory, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Infect Immun 69:5840-8. 2001
    ..gonorrhoeae...
  16. pmc Caveolin-1 modifies the immunity to Pseudomonas aeruginosa
    Mihaela Gadjeva
    Department of Medicine, Channing Laboratory, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 184:296-302. 2010
    ..aeruginosa infection in the setting of both acute pneumonia and chronic infection typical of CF...
  17. pmc Inflammation-related proteins in the blood of extremely low gestational age newborns. The contribution of inflammation to the appearance of developmental regulation
    Alan Leviton
    Neurology Department, Children s Hospital Boston, Harvard Medical School, Boston, MA, USA
    Cytokine 53:66-73. 2011
    ..We wanted to assess to what extent concentrations of circulating proteins appear to be developmentally regulated, and to what extent such regulation is influenced by intra-uterine inflammation...
  18. ncbi request reprint Response to Neisseria gonorrhoeae by cervicovaginal epithelial cells occurs in the absence of toll-like receptor 4-mediated signaling
    Raina N Fichorova
    Fearing Research Laboratory, Department of Obstetrics and Gynecology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Immunol 168:2424-32. 2002
    ..These data demonstrate that the response to N. gonorrhoeae and other Gram-negative bacteria at the mucosal surface of the female genital tract occurs in the absence of endotoxin recognition and TLR4-mediated signaling...
  19. ncbi request reprint Preclinical assessment of the proinflammatory potential of microbicide candidates
    Gustavo F Doncel
    CONRAD, Intramural Preclinical Research, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, VA 23507, USA
    J Acquir Immune Defic Syndr 37:S174-80. 2004
    ..This combined, sequential, preclinical evaluation system based on VK-2 cells in culture and a refined rabbit vaginal irritation model represents a valuable tool to assess the local safety profile of anti-HIV microbicide candidates...
  20. ncbi request reprint Biomarkers of leukocyte traffic and activation in the vaginal mucosa
    Radiana T Trifonova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Biomarkers 12:608-22. 2007
    ..These biomarkers, which were also detected in human vaginal secretions, may be used to enhance the characterization of mucosal safety of vaginally applied compounds, both in animal as well as clinical studies...
  21. pmc Guiding the vaginal microbicide trials with biomarkers of inflammation
    Raina N Fichorova
    Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Acquir Immune Defic Syndr 37:S184-93. 2004
    ....
  22. pmc Trichomonas vaginalis lipophosphoglycan triggers a selective upregulation of cytokines by human female reproductive tract epithelial cells
    Raina N Fichorova
    Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology and Reproductive Biology, Harvard Medical School, Brigham and Women s Hospital, 221 Longwood Avenue, RF468, Boston, MA 02115, USA
    Infect Immun 74:5773-9. 2006
    ..These effects were dose dependent and sustained in the absence of cytotoxicity and IL-1beta release and utilized, at least in part, a signaling pathway independent from the Toll-like/IL-1 receptor adaptor protein MyD88...
  23. ncbi request reprint Fourteen-day safety and acceptability study of 6% cellulose sulfate gel: a randomized double-blind Phase I safety study
    Jill L Schwartz
    Department of Obstetrics and Gynecology, CONRAD, Eastern Virginia Medical School, Arlington, VA 22209, USA
    Contraception 74:133-40. 2006
    ..Cellulose sulfate (CS) is a noncytotoxic antifertility agent that exhibits in vitro antimicrobial activity against sexually transmitted pathogens, including HIV...
  24. ncbi request reprint Detection of implantation-related cytokines in cervicovaginal secretions and peripheral blood of fertile women during ovulatory menstrual cycles
    Antonio R Gargiulo
    Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    Fertil Steril 82:1226-34. 2004
    ..To determine whether cytokines implicated in uterine receptivity are detectable in cervicovaginal secretions and/or serum of fertile women, and whether their concentrations undergo hormonal regulation during the menstrual cycle...
  25. ncbi request reprint Selective suppression of monocyte chemoattractant protein-1 expression by human papillomavirus E6 and E7 oncoproteins in human cervical epithelial and epidermal cells
    Kerstin Kleine-Lowinski
    Department of Medical Oncology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Int J Cancer 107:407-15. 2003
    ..These observations are consistent with a model in which MCP-1 expression by infected keratinocytes, which would stimulate an immune attack on HPV-transformed cells, is suppressed for invasive cervical cancer to appear...
  26. ncbi request reprint Expression and function of bactericidal/permeability-increasing protein in human genital tract epithelial cells
    Geraldine O Canny
    Center for Experimental Therapeutics and Reperfusion Injury, Department of Pathology, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, 02115, USA
    J Infect Dis 194:498-502. 2006
    ..The results of the present study suggest that BPI is tightly regulated and functionally expressed by epithelial cells in the female reproductive tract and may play a role in regulating bacterial colonization in the genital mucosa...

Research Grants4

  1. Trichomonas Lipophosphoglycan in Regulation Immune Responses
    Raina Fichorova; Fiscal Year: 2007
    ..vaginalis infections and complications. ..
  2. Molecular T. vaginalis-host interactions in relevance to inflammatory sequelae
    Raina Fichorova; Fiscal Year: 2009
    ..vaginalis-host interactions in relevance to inflammatory sequelae" is to unveil molecular mechanisms of parasite-host interactions associated with inflammatory symptoms and susceptibility to infection. ..
  3. Molecular T. vaginalis-host interactions in relevance to inflammatory sequelae
    Raina N Fichorova; Fiscal Year: 2010
    ..vaginalis-host interactions in relevance to inflammatory sequelae" is to unveil molecular mechanisms of parasite-host interactions associated with inflammatory symptoms and susceptibility to infection. ..