C H Evans

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Orthopedic gene therapy in 2008
    Christopher H Evans
    Center for Molecular Orthopaedics, Harvard Medical School, Boston, Massachusetts, USA
    Mol Ther 17:231-44. 2009
  2. doi Progress and Prospects: genetic treatments for disorders of bones and joints
    C H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Gene Ther 16:944-52. 2009
  3. doi Advances in regenerative orthopedics
    Christopher H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA Collaborative Research Center, AO Foundation, Davos, Switzerland Electronic address
    Mayo Clin Proc 88:1323-39. 2013
  4. pmc Arthritis gene therapy and its tortuous path into the clinic
    Christopher H Evans
    Department of Orthopedic Surgery, Harvard Medical School, Boston, Mass, USA
    Transl Res 161:205-16. 2013
  5. pmc Gene delivery to bone
    C H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Adv Drug Deliv Rev 64:1331-40. 2012
  6. pmc Effects of short-term glucocorticoid treatment on changes in cartilage matrix degradation and chondrocyte gene expression induced by mechanical injury and inflammatory cytokines
    Yihong C S Lu
    Department of Biological Engineering, MIT, 500 Technology Square NE47 377, Cambridge, MA 02139, USA
    Arthritis Res Ther 13:R142. 2011
  7. pmc Barriers to the clinical translation of orthopedic tissue engineering
    Christopher H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Tissue Eng Part B Rev 17:437-41. 2011
  8. pmc Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer
    Andre F Steinert
    Orthopaedic Center for Musculoskeletal Research, Orthopaedic Clinic, König Ludwig Haus, Julius Maximilians University, Brettreichstrasse 11, 97074 Wurzburg, Germany
    Arthritis Res Ther 11:R148. 2009
  9. ncbi Use of genetically modified muscle and fat grafts to repair defects in bone and cartilage
    C H Evans
    Center for Molecular Orthopaedics, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Eur Cell Mater 18:96-111. 2009
  10. pmc Gene therapy of the rheumatic diseases: 1998 to 2008
    Christopher H Evans
    Center for Advanced Orthopaedic Studies, Harvard Medical School, BIDMC RN115, 330 Brookline Avenue, Boston, MA 02215, USA
    Arthritis Res Ther 11:209. 2009

Research Grants

  1. A Biological Basis for Repair of the ACL
    Christopher Evans; Fiscal Year: 2009
  2. Healing of Segmental Defects of Bone by Gene Transfer
    Christopher H Evans; Fiscal Year: 2010
  3. Transfer of IL-1Ra cDNA to Osteoarthritic Knee
    Christopher Evans; Fiscal Year: 2007
  4. Healing of Segmental Defects in Bone by Gene Transfer
    Christopher Evans; Fiscal Year: 2007

Collaborators

Detail Information

Publications46

  1. pmc Orthopedic gene therapy in 2008
    Christopher H Evans
    Center for Molecular Orthopaedics, Harvard Medical School, Boston, Massachusetts, USA
    Mol Ther 17:231-44. 2009
    ....
  2. doi Progress and Prospects: genetic treatments for disorders of bones and joints
    C H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Gene Ther 16:944-52. 2009
    ..For tissue repair, the major research questions are still which genes to use and how best to deliver them...
  3. doi Advances in regenerative orthopedics
    Christopher H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA Collaborative Research Center, AO Foundation, Davos, Switzerland Electronic address
    Mayo Clin Proc 88:1323-39. 2013
    ..More expeditious approaches make full use of intrinsic biological processes in vivo to avoid the need for ex vivo expansion of autologous cells and multiple procedures. Clinical translation remains a bottleneck. ..
  4. pmc Arthritis gene therapy and its tortuous path into the clinic
    Christopher H Evans
    Department of Orthopedic Surgery, Harvard Medical School, Boston, Mass, USA
    Transl Res 161:205-16. 2013
    ..It is to be hoped that the recent successes in treating rare, Mendelian diseases by gene therapy will lead to accelerated development of genetic treatments for common, non-Mendelian diseases, such as arthritis...
  5. pmc Gene delivery to bone
    C H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    Adv Drug Deliv Rev 64:1331-40. 2012
    ..These include healing large segmental defects in long bones and the cranium, as well as spinal fusion and treating avascular necrosis...
  6. pmc Effects of short-term glucocorticoid treatment on changes in cartilage matrix degradation and chondrocyte gene expression induced by mechanical injury and inflammatory cytokines
    Yihong C S Lu
    Department of Biological Engineering, MIT, 500 Technology Square NE47 377, Cambridge, MA 02139, USA
    Arthritis Res Ther 13:R142. 2011
    ....
  7. pmc Barriers to the clinical translation of orthopedic tissue engineering
    Christopher H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Tissue Eng Part B Rev 17:437-41. 2011
    ..Some strategies for achieving this are suggested...
  8. pmc Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer
    Andre F Steinert
    Orthopaedic Center for Musculoskeletal Research, Orthopaedic Clinic, König Ludwig Haus, Julius Maximilians University, Brettreichstrasse 11, 97074 Wurzburg, Germany
    Arthritis Res Ther 11:R148. 2009
    ..The present study compares bone morphogenetic protein (BMP)-4 and BMP-2 gene transfer as agents of chondrogenesis and hypertrophy in human primary mesenchymal stem cells (MSCs) maintained as pellet cultures...
  9. ncbi Use of genetically modified muscle and fat grafts to repair defects in bone and cartilage
    C H Evans
    Center for Molecular Orthopaedics, Brigham and Women s Hospital, Harvard Medical School, Boston, MA, USA
    Eur Cell Mater 18:96-111. 2009
    ..Further development of these methods should provide ways to heal bone and cartilage more expeditiously, and at lower cost, than is presently possible...
  10. pmc Gene therapy of the rheumatic diseases: 1998 to 2008
    Christopher H Evans
    Center for Advanced Orthopaedic Studies, Harvard Medical School, BIDMC RN115, 330 Brookline Avenue, Boston, MA 02215, USA
    Arthritis Res Ther 11:209. 2009
    ..The translational research necessary to turn these advances into effective genetic medicines requires sustained funding and continuity of effort...
  11. doi Gene therapy for bone healing
    Christopher H Evans
    Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, RN 115, Boston, MA 02215, USA
    Expert Rev Mol Med 12:e18. 2010
    ..Thus, progress in developing a clinically useful gene therapy for bone healing is determined not only by scientific considerations, but also by financial constraints and the ambient regulatory environment...
  12. pmc Getting arthritis gene therapy into the clinic
    Christopher H Evans
    Center for Advanced Orthopedic Studies, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Avenue, RN 115, Boston, MA 02215, USA
    Nat Rev Rheumatol 7:244-9. 2011
    ..This article summarizes the status in 2010 of the clinical development of gene therapy for arthritis, identifies certain constraints to progress and suggests possible solutions...
  13. pmc Gene therapy for the regeneration of bone
    Christopher Evans
    Center for Advanced Orthopaedic Studies, Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center, BIDMC RN 115, 330, Brookline Avenue, Boston, MA 02215, United States
    Injury 42:599-604. 2011
    ..Nevertheless, the overall climate for gene therapy is improving, permitting optimism that applications in bone regeneration will eventually become available...
  14. pmc Orthopedic gene therapy--lost in translation?
    C H Evans
    Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
    J Cell Physiol 227:416-20. 2012
    ..We hope that orthopedic applications will benefit collaterally from this upswing and move expeditiously into advanced clinical trials...
  15. ncbi Human intervertebral disc cells are genetically modifiable by adenovirus-mediated gene transfer: implications for the clinical management of intervertebral disc disorders
    S H Moon
    Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Spine (Phila Pa 1976) 25:2573-9. 2000
    ....
  16. doi A simple, lanthanide-based method to enhance the transduction efficiency of adenovirus vectors
    G D Palmer
    Center for Molecular Orthopaedics, Harvard Medical School, Boston, MA 2115, USA
    Gene Ther 15:357-63. 2008
    ..Our findings suggest that this lanthanide-based method holds much promise for expediting both experimental and clinical applications of gene transfer with adenoviral vectors...
  17. ncbi Protective effects of IL-1Ra or vIL-10 gene transfer on a murine model of wear debris-induced osteolysis
    S Y Yang
    Department of Orthopaedic Surgery, Wayne State University, Detroit, MI, USA
    Gene Ther 11:483-91. 2004
    ....
  18. ncbi Toward a biochemical understanding of human intervertebral disc degeneration and herniation. Contributions of nitric oxide, interleukins, prostaglandin E2, and matrix metalloproteinases
    J D Kang
    Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, Pennsylvania, USA
    Spine (Phila Pa 1976) 22:1065-73. 1997
    ..Endogenously produced nitric oxide appears to have a strong inhibitory effect on the production of interleukin-6, which suggests that autocrine mechanisms play an important role in the regulation of disc cell metabolism...
  19. ncbi Herniated lumbar intervertebral discs spontaneously produce matrix metalloproteinases, nitric oxide, interleukin-6, and prostaglandin E2
    J D Kang
    Department of Orthopaedic Surgery, University of Pittsburgh Medical Center, Pennsylvania, USA
    Spine (Phila Pa 1976) 21:271-7. 1996
    ..Their exact roles certainly need further investigation, but their mere presence implicates biochemical processes in intervertebral disc degeneration...
  20. ncbi Adenovirus-mediated gene transfer to nucleus pulposus cells. Implications for the treatment of intervertebral disc degeneration
    K Nishida
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pennsylvania, USA
    Spine (Phila Pa 1976) 23:2437-42; discussion 2443. 1998
    ....
  21. ncbi Effects of cytokine gene therapy on particulate-induced inflammation in the murine air pouch
    S Sud
    Department of Orthopaedic Surgery, Wayne State University School of Medicine, Detroit, Ml 48201, USA
    Inflammation 25:361-72. 2001
    ..The data suggest that the air pouch model represents a useful tool to evaluate gene therapy, and demonstrate that IL-1Ra gene therapy may be an appropriate therapeutic approach to inflammation...
  22. ncbi Adenovirus-mediated gene transfer of glutamine: fructose-6-phosphate amidotransferase antagonizes the effects of interleukin-1beta on rat chondrocytes
    J N Gouze
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    Osteoarthritis Cartilage 12:217-24. 2004
    ..To determine whether overexpression of glutamine: fructose-6-phosphate amidotransferase (GFAT) in synoviocytes will antagonize the response to interleukin-1beta (IL-1beta) of chondrocytes and synovial fibroblasts in co-culture...
  23. ncbi Gene delivery to cartilage defects using coagulated bone marrow aspirate
    A Pascher
    Center for Molecular Orthopaedics, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Gene Ther 11:133-41. 2004
    ....
  24. ncbi Osteoarthritis gene therapy
    C H Evans
    Center for Molecular Orthopaedics, Harvard Medical School, MA 02115, USA
    Gene Ther 11:379-89. 2004
    ..Of the available vector systems, recombinant adeno-associated virus may provide the best combination of safety with in vivo delivery using current technology...
  25. ncbi Gene therapy for rheumatoid arthritis
    J N Gouze
    Department of Orthopedic Surgery, Harvard Medical School, Boston, MA, USA
    Hand Surg 6:211-9. 2001
    ..This study has confirmed that it is possible to transfer genes safely to human joints. It should pave the way for additional application of gene therapy to arthritis and other orthopaedic conditions...
  26. ncbi Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects
    O B Betz
    Center for Molecular Orthopaedics, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
    Gene Ther 14:1039-44. 2007
    ..BMP-2 until 5 or 10 days after surgery enables a greater percentage of critical-sized, segmental defects to achieve radiological union, producing a repair tissue with enhanced mineralization and greater mechanical strength...
  27. ncbi Cartilage injury and repair
    S C Ghivizzani
    Department of Orthopaedic Surgery, Harvard Medical School, Boston, Massachusetts, USA
    Phys Med Rehabil Clin N Am 11:289-307, vi. 2000
    ....
  28. ncbi Gene therapeutic approaches-transfer in vivo
    C H Evans
    Center for Molecular Orthopaedics, Harvard Medical School, Boston, MA 02115, USA
    Adv Drug Deliv Rev 58:243-58. 2006
    ..Adeno-associated virus has been used in a phase I study for the gene therapy of rheumatoid arthritis. Its use in a clinical trial for treating OA is pending...
  29. ncbi Intermittent sub-ambient interstitial hydrostatic pressure as a potential mechanical stimulator for chondrocyte metabolism
    J K Suh
    Department of Orthopaedic Surgery, University of Pittsburgh, PA 15213, USA
    Osteoarthritis Cartilage 7:71-80. 1999
    ....
  30. ncbi Gene therapy for rheumatoid arthritis
    E Gouze
    Center for Molecular Orthopaedics, Harvard Medical School, Boston, MA 02115, USA
    Expert Opin Biol Ther 1:971-8. 2001
    ..This clinical trial has been successfully completed and two other Phase I trials are in progress. A Phase II study is now being planned to investigate the efficacy of gene transfer to the joints of patients with early stage RA...
  31. ncbi Modulation of the biologic activity of the rabbit intervertebral disc by gene therapy: an in vivo study of adenovirus-mediated transfer of the human transforming growth factor beta 1 encoding gene
    K Nishida
    Department of Orthopaedic Surgery, University of Pittsburgh, Pennsylvania, USA
    Spine (Phila Pa 1976) 24:2419-25. 1999
    ..Gene therapy therefore may have useful applications for study of the basic science of the intervertebral disc and for clinical management of degenerative disc disease...
  32. ncbi Nitric oxide inhibits chondrocyte response to IGF-I: inhibition of IGF-IRbeta tyrosine phosphorylation
    R K Studer
    Ferguson Laboratory, Musculoskeletal Research Center, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Am J Physiol Cell Physiol 279:C961-9. 2000
    ..These studies show that NO is responsible for part of arthritic cartilage/chondrocyte insensitivity to anabolic actions of IGF-I; inhibition of receptor autophosphorylation is potentially responsible for this effect...
  33. pmc Lessons learned from gene transfer approaches
    C H Evans
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Arthritis Res 1:21-4. 1999
    ..This article reviews briefly the results of experiments in which selected genes have been transferred to the knee joints of healthy rabbits and rabbits with antigen-induced arthritis...
  34. ncbi Gene therapy for rheumatoid arthritis
    E Gouze
    Center for Molecular Orthopaedics, Harvard Medical School, 221 Longwood Avenue, BL-152, Boston, MA 02115, USA
    Curr Rheumatol Rep 3:79-85. 2001
    ..Limited duration of gene expression impedes the development of a clinically useful genetic treatment for arthritis...
  35. pmc Future of adenoviruses in the gene therapy of arthritis
    C H Evans
    Center for Molecular Orthopaedics, Harvard Medical School, Boston, MA 02115, USA
    Arthritis Res 3:142-6. 2001
    ..Whether these will offer advantages over existing vectors, which may already provide safe, long-term gene expression following in vivo delivery, remains to be seen...
  36. ncbi Gene therapy for autoimmune disorders
    C H Evans
    Center for Molecular Orthopaedics, Department of Orthopaedic Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Clin Immunol 20:334-46. 2000
    ..Two additional clinical trials are in progress. It is likely that gene therapy will provide effective new treatments for a wide range of autoimmune disorders...
  37. ncbi Blocking cytokines with genes
    C H Evans
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pennsylvania, USA
    J Leukoc Biol 64:55-61. 1998
    ..The success of these laboratory studies has led to the implementation of a Phase I clinical trial to asses the safety and feasibility of using gene therapy in the treatment of RA...
  38. ncbi Potential treatment of osteoarthritis by gene therapy
    C H Evans
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pennsylvania, USA
    Rheum Dis Clin North Am 25:333-44. 1999
    ..These genes may be transferred to the synovium or cartilage of affected joints by in vivo or ex vivo means using a variety of vectors. Transfer of such genes to chondroprogenitor cells is a particularly attractive approach...
  39. ncbi Design, characterisation and in vivo testing of a new, adjustable stiffness, external fixator for the rat femur
    V Glatt
    Center for Advanced Orthopaedic Studies, Harvard Medical School, Boston, MA
    Eur Cell Mater 23:289-98; discussion 299. 2012
    ..Our data suggest that, if alteration of the mechanical environment is to be used to modulate the healing of large segmental defects, this needs to be performed before the tissue properties become dominant...
  40. ncbi Herniated cervical intervertebral discs spontaneously produce matrix metalloproteinases, nitric oxide, interleukin-6, and prostaglandin E2
    J D Kang
    Musculoskeletal Research Center, Ferguson Laboratory, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, PA, USA
    Spine (Phila Pa 1976) 20:2373-8. 1995
    ..These products may be intimately involved in the biochemistry of disc degeneration and the pathophysiology of radiculopathy...
  41. ncbi Delivery of plasmid DNA to articular chondrocytes via novel collagen-glycosaminoglycan matrices
    R E Samuel
    Department of Orthopedic Surgery, Brigham and Women s Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115, USA
    Hum Gene Ther 13:791-802. 2002
    ..Thus, GSCG matrices can be fabricated to provide sustained release of plasmid DNA carrying a potential therapeutic gene...
  42. pmc Approaches to enhancing the retroviral transduction of human synoviocytes
    M A Del Vecchio
    Department of Human Genetics, Graduate School of Public Health, University of Pittsburgh, PA, USA
    Arthritis Res 3:259-63. 2001
    ..However, the shortfall could be remedied by increasing the time of transduction under static conditions, transducing under flow-through conditions, or transducing during centrifugation...
  43. ncbi IL-1Ra and vIL-10 gene transfer using retroviral vectors ameliorates particle-associated inflammation in the murine air pouch model
    S Yang
    Department of Orthopaedic Surgery, Wayne State University and the John D Dingle VA Medical Center, Hutzel Hospital, Detroit, MI 48201, USA
    Inflamm Res 51:342-50. 2002
    ..This study examined anti- inflammatory gene therapy to ameliorate tissue responses to ultra high molecular weight polyethylene (UHMWPE) particles in the murine air pouch...
  44. ncbi Effect of rhBMP-2 on the osteogenic potential of bone marrow stromal cells from an osteogenesis imperfecta mouse (oim)
    M L Balk
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Bone 21:7-15. 1997
    ..Under basal conditions, however, the stromal cells from oim mice exhibited significantly lower levels of alkaline phosphatase activity than their normal littermates...
  45. ncbi On the TRAIL of an arthritis cure
    C H Evans
    Center for Molecular Orthopaedics, Harvard Medical School, USA
    Gene Ther 11:735-6. 2004
  46. ncbi Cytokines and the role they play in the healing of ligaments and tendons
    C H Evans
    Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA
    Sports Med 28:71-6. 1999
    ..Slow release devices and gene transfer are being evaluated as strategies for obviating this limitation...

Research Grants14

  1. A Biological Basis for Repair of the ACL
    Christopher Evans; Fiscal Year: 2009
    ..MRI, histology and immunohistochemistry will also be used to evaluate the healed ligament. These studies will advance our knowledge of the biology of the injured ACL and suggest novel, biologically-based approaches to healing. ..
  2. Healing of Segmental Defects of Bone by Gene Transfer
    Christopher H Evans; Fiscal Year: 2010
    ..The method involves the converting muscle into bone by transferring a gene encoding bone morphogenetic protein- 2 using an adenovirus. ..
  3. Transfer of IL-1Ra cDNA to Osteoarthritic Knee
    Christopher Evans; Fiscal Year: 2007
    ..abstract_text> ..
  4. Healing of Segmental Defects in Bone by Gene Transfer
    Christopher Evans; Fiscal Year: 2007
    ..The results of this project will serve as the basis for planning early phase clinical protocols, or will have identified the critical, key matters that future research needs to address prior to developing such protocols. ..