ALAN N ENGELMAN

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint The roles of cellular factors in retroviral integration
    A Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Curr Top Microbiol Immunol 281:209-38. 2003
  2. pmc The SET complex acts as a barrier to autointegration of HIV-1
    Nan Yan
    Department of Pediatrics, Immune Disease Institute, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 5:e1000327. 2009
  3. pmc A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo
    Fanny Turlure
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Nucleic Acids Res 34:1653-65. 2006
  4. pmc A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, London, United Kingdom
    PLoS Pathog 5:e1000259. 2009
  5. pmc Structural basis for functional tetramerization of lentiviral integrase
    Stephen Hare
    Division of Medicine, St Mary s Campus, Imperial College London, London, United Kingdom
    PLoS Pathog 5:e1000515. 2009
  6. pmc HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor
    Hao Wang
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA
    Nucleic Acids Res 40:11518-30. 2012
  7. pmc Biochemical characterization of novel retroviral integrase proteins
    Allison Ballandras-Colas
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 8:e76638. 2013
  8. pmc Nucleoporin NUP153 phenylalanine-glycine motifs engage a common binding pocket within the HIV-1 capsid protein to mediate lentiviral infectivity
    Kenneth A Matreyek
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 9:e1003693. 2013
  9. pmc Viral and cellular requirements for the nuclear entry of retroviral preintegration nucleoprotein complexes
    Kenneth A Matreyek
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Viruses 5:2483-511. 2013
  10. pmc Allosteric inhibition of HIV-1 integrase activity
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 450 Brookline Avenue, CLS 1010, Boston, MA 02215, USA
    Curr Opin Chem Biol 17:339-45. 2013

Research Grants

  1. HIV-1 Preintegration Trafficking and Nuclear Localization
    Alan Engelman; Fiscal Year: 2007
  2. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2009
  3. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2005
  4. Nuclear Localization of HIV-1 Preintegration Complexes
    Alan Engelman; Fiscal Year: 2006
  5. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2006
  6. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2006
  7. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2007
  8. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2007
  9. HIV-1 Preintegration Trafficking and Nuclear Localization
    Alan Engelman; Fiscal Year: 2009
  10. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009

Collaborators

Detail Information

Publications49

  1. ncbi request reprint The roles of cellular factors in retroviral integration
    A Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Curr Top Microbiol Immunol 281:209-38. 2003
    ..The potential to interfere with proper integration by blocking either integrase catalysis or the function of cellular integration cofactors is also discussed...
  2. pmc The SET complex acts as a barrier to autointegration of HIV-1
    Nan Yan
    Department of Pediatrics, Immune Disease Institute, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 5:e1000327. 2009
    ..Therefore, the SET complex facilitates HIV-1 infection by preventing suicidal autointegration...
  3. pmc A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo
    Fanny Turlure
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Nucleic Acids Res 34:1653-65. 2006
    ....
  4. pmc A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75
    Stephen Hare
    Division of Medicine, Imperial College London, St Mary s Campus, London, United Kingdom
    PLoS Pathog 5:e1000259. 2009
    ..These findings provide structural basis for the high affinity lentiviral IN-LEDGF interaction and pave the way for development of LEDGF-based targeting technologies for gene therapy...
  5. pmc Structural basis for functional tetramerization of lentiviral integrase
    Stephen Hare
    Division of Medicine, St Mary s Campus, Imperial College London, London, United Kingdom
    PLoS Pathog 5:e1000515. 2009
    ..Our structures moreover highlight adaptable changes at the interfaces of individual IN dimers that allow divergent lentiviruses to utilize a highly-conserved, common integration co-factor...
  6. pmc HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor
    Hao Wang
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA
    Nucleic Acids Res 40:11518-30. 2012
    ..These findings help to rule out the host factor-IN interactions as the primary antiviral targets of LEDGF/p75-binding site IN inhibitors...
  7. pmc Biochemical characterization of novel retroviral integrase proteins
    Allison Ballandras-Colas
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 8:e76638. 2013
    ..Given their desirable concerted DNA integration profiles, Rev-A and MMTV integrase proteins have been earmarked for structural biology studies. ..
  8. pmc Nucleoporin NUP153 phenylalanine-glycine motifs engage a common binding pocket within the HIV-1 capsid protein to mediate lentiviral infectivity
    Kenneth A Matreyek
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Pathog 9:e1003693. 2013
    ..We conclude that a subset of lentiviral CA proteins directly engage FG-motifs present on NUP153 to affect viral nuclear import...
  9. pmc Viral and cellular requirements for the nuclear entry of retroviral preintegration nucleoprotein complexes
    Kenneth A Matreyek
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA
    Viruses 5:2483-511. 2013
    ..In this review, we describe our current understanding of retroviral nuclear import, with emphasis on recent developments on the role of the HIV-1 capsid protein. ..
  10. pmc Allosteric inhibition of HIV-1 integrase activity
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 450 Brookline Avenue, CLS 1010, Boston, MA 02215, USA
    Curr Opin Chem Biol 17:339-45. 2013
    ..ALLINIs display steep dose response curves and synergize with INSTIs ex vivo, highlighting this novel inhibitor class for clinical development...
  11. pmc The structural biology of HIV-1: mechanistic and therapeutic insights
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
    Nat Rev Microbiol 10:279-90. 2012
    ..Here, we review recent advances in HIV-1 structural biology, focusing on the molecular mechanisms of viral replication and on the development of new therapeutics...
  12. pmc Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus
    Xiang Li
    Address correspondence to Alan Engelman
    J Virol 86:3861-79. 2012
    ....
  13. pmc Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase
    Mohd J Dar
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA, USA
    Retrovirology 6:94. 2009
    ..The F185K core domain mutation, which increases integrase protein solubility, was furthermore analyzed in a subset of mutants...
  14. pmc The ups and downs of gene expression and retroviral DNA integration
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:1275-6. 2005
  15. ncbi request reprint AIDS/HIV. A reversal of fortune in HIV-1 integration
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 316:1855-7. 2007
  16. pmc Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75
    Peter Cherepanov
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:17308-13. 2005
    ....
  17. pmc The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Pathog 4:e1000046. 2008
    ....
  18. pmc Quantitative analysis of HIV-1 preintegration complexes
    Alan Engelman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Division of AIDS, Harvard Medical School, 44 Binney Street, CLSB 1010, Boston, MA 02115, USA
    Methods 47:283-90. 2009
    ..This study details HIV-1 infection, PIC extraction, partial purification, and quantitative analyses of integrase 3' processing and DNA strand transfer activities...
  19. ncbi request reprint Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication
    Nick Vandegraaff
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Virology 346:415-26. 2006
    ..We conclude that cells knocked-out for LEDGF/p75 and/or HRP2 will be useful genetic tools to address the roles of these host cell factors in HIV-1 replication...
  20. ncbi request reprint Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness
    Shaila Rahman
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Virology 357:79-90. 2007
    ..Thus, the relative affinity of the in vitro IN-LEDGF/p75 interaction is not a universal predictor of IN mutant viral fitness...
  21. pmc The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase
    Lavanya Krishnan
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    J Virol 84:397-406. 2010
    ..We therefore conclude that capsid, not integrase, is the dominant viral factor that dictates transportin 3 dependency during HIV-1 infection...
  22. doi request reprint Identification of host proteins required for HIV infection through a functional genomic screen
    Abraham L Brass
    Department of Genetics, Center for Genetics and Genomics, Brigham and Women s Hospital, Howard Hughes Medical Institute, Harvard Medical School, Boston, MA 02115, USA
    Science 319:921-6. 2008
    ..This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing identify potential targets for therapy...
  23. pmc LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration
    Ming Chieh Shun
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 21:1767-78. 2007
    ..Akin to yeast retrotransposons, disrupting the lentiviral targeting mechanism significantly perturbs overall integration...
  24. pmc Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity
    Ming Chieh Shun
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute and Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    J Virol 82:11555-67. 2008
    ..This initial systematic mutagenesis of a PWWP domain identifies amino acid residues critical for chromatin binding function and the consequences of their changes on HIV-1 integration and infection...
  25. pmc Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import
    Ana Limon
    Department of Pathology, Harvard Medical School, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    J Virol 76:10598-607. 2002
    ....
  26. ncbi request reprint Human cell proteins and human immunodeficiency virus DNA integration
    Fanny Turlure
    Department of Pathology, Harvard Medical School, Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Front Biosci 9:3187-208. 2004
    ..We also present experimental results of human cell proteins that coimmunoprecipitated with HIV-1 IN following its expression in HeLa cells and discuss these results in light of the previously-identified integration cofactors...
  27. pmc LEDGF/p75 interferes with the formation of synaptic nucleoprotein complexes that catalyze full-site HIV-1 DNA integration in vitro: implications for the mechanism of viral cDNA integration
    Nidhanapati K Raghavendra
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Virology 360:1-5. 2007
    ..We therefore speculate that LEDGF/p75 function is restricted to a point in the viral lifecycle that occurs after the formation of the preintegration synaptic complex, for example, as a chromatin-associated tethering factor...
  28. ncbi request reprint Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase
    Peter Cherepanov
    Departments of Cancer Immunology and AIDS and Cancer Biology, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    J Biol Chem 279:48883-92. 2004
    ..LEDGF/p75 and HRP2 are predicted to share a similar domain organization and have an evident evolutionary and likely functional relationship...
  29. pmc Wild-type levels of nuclear localization and human immunodeficiency virus type 1 replication in the absence of the central DNA flap
    Ana Limon
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 76:12078-86. 2002
    ..Our results indicate that the central DNA flap does not play a major role in either preintegration complex nuclear import or HIV-1 replication in a variety of cell types...
  30. pmc The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes
    Chou Wen Lin
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 77:5030-6. 2003
    ..These data are consistent with the model that BAF functions as an integration cofactor in vivo...
  31. pmc Simian virus 40-based replication of catalytically inactive human immunodeficiency virus type 1 integrase mutants in nonpermissive T cells and monocyte-derived macrophages
    Richard Lu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Virol 78:658-68. 2004
    ..SV40-based HIV-1 integrase mutant replication in otherwise nonpermissive cells suggests alternative approaches to standard integrase-mediated retroviral gene transfer strategies...
  32. ncbi request reprint Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75
    Goedele Maertens
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    J Biol Chem 279:33421-9. 2004
    ..Moreover, a single amino acid change in the NLS was sufficient to exclude the mutant LEDGF/p75 protein from the nucleus and abolish nuclear import of HIV-1 integrase...
  33. pmc Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication
    Richard Lu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney St, Boston, MA 02115, USA
    J Virol 78:12735-46. 2004
    ..Since HIV-1(C130A) grew like the wild type, we conclude that Cys-130 is not essential for replication and speculate that perturbation of integrase structure contributed to the pleiotropic HIV-1(C130G) phenotype...
  34. ncbi request reprint Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75
    Peter Cherepanov
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 12:526-32. 2005
    ..The initial structure determination of a host cell factor that tightly binds to a retroviral enzyme lays the groundwork for understanding enzyme-host interactions important for viral replication...
  35. ncbi request reprint Transcriptional co-activator p75 binds and tethers the Myc-interacting protein JPO2 to chromatin
    Goedele N Maertens
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    J Cell Sci 119:2563-71. 2006
    ..Our results suggest a role for p75 in the Myc regulatory network, and indicate that p75 is a general adaptor protein tethering divergent factors to chromatin through its versatile integrase-binding domain...
  36. pmc Genetic analyses of conserved residues in the carboxyl-terminal domain of human immunodeficiency virus type 1 integrase
    Richard Lu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    J Virol 79:10356-68. 2005
    ....
  37. ncbi request reprint Intracellular transport of human immunodeficiency virus type 1 integrase
    Eric Devroe
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    J Cell Sci 116:4401-8. 2003
    ..These results provide new insight into human immunodeficiency virus type 1 integrase intracellular dynamics...
  38. ncbi request reprint Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1
    Felipe Diaz-Griffero
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    Virology 351:404-19. 2006
    ....
  39. pmc Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase
    Richard Lu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney St Boston, MA 02115, USA
    J Virol 79:2493-505. 2005
    ..The results of these analyses highlight conserved CCD residues that are important for HIV-1 replication and integration and define the relationship between DNA binding and catalysis that occurs during integration in vivo...
  40. pmc Lys-34, dispensable for integrase catalysis, is required for preintegration complex function and human immunodeficiency virus type 1 replication
    Richard Lu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
    J Virol 79:12584-91. 2005
    ..We therefore concluded that mutations like K34A disrupted higher-order interactions important for PIC function/maturation compared to the innate catalytic activity of IN enzyme...
  41. pmc Structure-based modeling of the functional HIV-1 intasome and its inhibition
    Lavanya Krishnan
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 107:15910-5. 2010
    ..Our findings highlight the structural basis for HIV-1 integration and define the mechanism of its inhibition, which should help in formulating new drugs to inhibit viruses resistant to first-in-class compounds...
  42. doi request reprint Isolation and analysis of HIV-1 preintegration complexes
    Alan Engelman
    Department of Cancer Immunology, Dana Farber Cancer Institute and Division of AIDS, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 485:135-49. 2009
    ..This chapter describes techniques for isolating HIV-1 PICs from cells as well as quantifying their level of integration activity in vitro...
  43. ncbi request reprint HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases
    Eric Devroe
    Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA
    Virology 331:181-9. 2005
    ..These studies identify two new virion-associated host cell enzymes and suggest a novel mechanism by which HIV-1 alters the intracellular environment of human cells...
  44. pmc Differential sensitivities of retroviruses to integrase strand transfer inhibitors
    Yasuhiro Koh
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney Street, CLS 1010, Boston, MA 02115, USA
    J Virol 85:3677-82. 2011
    ..The drugs intermediately inhibited intracisternal A-particle retrotransposition but were inactive against Sleeping Beauty transposition and long interspersed nucleotide element 1 (LINE-1) retrotransposition...
  45. pmc The requirement for nucleoporin NUP153 during human immunodeficiency virus type 1 infection is determined by the viral capsid
    Kenneth A Matreyek
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 450 Brookline Avenue, CLS 1010, Boston, MA 02215, USA
    J Virol 85:7818-27. 2011
    ..These results suggest that capsid, likely by the qualities of its uncoating, determines whether HIV-1 requires cellular NUP153 for PIC nuclear import...
  46. pmc Identification and characterization of persistent intracellular human immunodeficiency virus type 1 integrase strand transfer inhibitor activity
    Yasuhiro Koh
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Antimicrob Agents Chemother 55:42-9. 2011
    ..Combined intracellular persistence and virucidal activities suggest potential pre-exposure prophylaxis applications for EVG...
  47. pmc Evolution of a cytoplasmic tripartite motif (TRIM) protein in cows that restricts retroviral infection
    Zhihai Si
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:7454-9. 2006
    ..Particular features of this subset of cytoplasmic TRIM proteins may be conducive to the convergent evolution of virus-restricting factors...
  48. doi request reprint General guidelines for experimenting with HIV
    Karen B Byers
    Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Curr Protoc Immunol . 2004
    ..The first protocol in this unit establishes a general framework for working safely with HIV and the second describes the proper storage of HIV stocks and test supernatants...
  49. pmc Wild-type levels of human immunodeficiency virus type 1 infectivity in the absence of cellular emerin protein
    Ming Chieh Shun
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney St, Boston, MA 02115, USA
    J Virol 81:166-72. 2007
    ....

Research Grants34

  1. HIV-1 Preintegration Trafficking and Nuclear Localization
    Alan Engelman; Fiscal Year: 2007
    ..This knowledge in the long run will define novel targets for therapeutic interaction to develop new anti-viral drugs in the fight against HIV/AIDS. ..
  2. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  3. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..
  4. Nuclear Localization of HIV-1 Preintegration Complexes
    Alan Engelman; Fiscal Year: 2006
    ..abstract_text> ..
  5. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2006
    ..abstract_text> ..
  6. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2006
    ....
  7. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2007
    ....
  8. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2007
    ..abstract_text> ..
  9. HIV-1 Preintegration Trafficking and Nuclear Localization
    Alan Engelman; Fiscal Year: 2009
    ..This knowledge in the long run will define novel targets for therapeutic interaction to develop new anti-viral drugs in the fight against HIV/AIDS. ..
  10. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  11. HIV-1 Preintegration Trafficking and Nuclear Localization
    ALAN N ENGELMAN; Fiscal Year: 2010
    ..This knowledge in the long run will define novel targets for therapeutic interaction to develop new anti-viral drugs in the fight against HIV/AIDS. ..
  12. HIV-1 Integrase Structural Biology
    ALAN N ENGELMAN; Fiscal Year: 2010
    ..abstract_text> ..
  13. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2004
    ..The results of these experiments will be used to formulate a detailed model of the structural and functional organization of HIV-1 PICs, which will aid the design of antiviral drugs targeted against HIV-1 integration. ..
  14. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2000
    ..The results of these experiments will be used to formulate a detailed model of the structural and functional organization of HIV-1 PICs, which will aid the design of antiviral drugs targeted against HIV-1 integration. ..
  15. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2001
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  16. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2002
    ..The results of these experiments will be used to formulate a detailed model of the structural and functional organization of HIV-1 PICs, which will aid the design of antiviral drugs targeted against HIV-1 integration. ..
  17. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2002
    ..The results of these experiments will be used to formulate a detailed model of the structural and functional organization of HIV-1 PICs, which will aid the design of antiviral drugs targeted against HIV-1 integration. ..
  18. Nuclear Localization of HIV-1 Preintegration Complexes
    Alan Engelman; Fiscal Year: 2003
    ..abstract_text> ..
  19. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2003
    ..The results of these experiments will be used to formulate a detailed model of the structural and functional organization of HIV-1 PICs, which will aid the design of antiviral drugs targeted against HIV-1 integration. ..
  20. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 1999
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  21. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 1999
    ..C. Examination of higher order protein-protein and protein-DNA interactions important for preintegration complex activity. D. Develop an assay for authentic integration of exogenous HIV-1 DNA. ..
  22. HIV-1 Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2009
    ..abstract_text> ..
  23. MECHANISM OF NON-INTEGRASE-MEDIATED HIV-1 REPLICATION
    Alan Engelman; Fiscal Year: 2000
    ..Further aims are to develop gene therapy vectors based on non-integrating retroviruses. ..
  24. BIOCHEMICAL MECHANISM OF HIV DNA INTEGRATION
    Alan Engelman; Fiscal Year: 2001
    ..The results of these experiments will be used to formulate a detailed model of the structural and functional organization of HIV-1 PICs, which will aid the design of antiviral drugs targeted against HIV-1 integration. ..
  25. LEDGF-Integrase Structural Biology
    Alan Engelman; Fiscal Year: 2005
    ..abstract_text> ..