ELAINE ELION

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Making protein immunoprecipitates
    Elaine A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 284:1-14. 2004
  2. pmc Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene
    Xiaoyan Wang
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    BMC Mol Biol 12:51. 2011
  3. doi request reprint Analysis of mitogen-activated protein kinase activity in yeast
    Elaine A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 661:387-99. 2010
  4. ncbi request reprint Signal transduction. Signaling specificity in yeast
    Elaine A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Science 307:687-8. 2005
  5. ncbi request reprint The Ste5p scaffold
    E A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 114:3967-78. 2001
  6. ncbi request reprint far4, far5, and far6 define three genes required for efficient activation of MAPKs Fus3 and Kss1 and accumulation of glycogen
    V Cherkasova
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Curr Genet 40:13-26. 2001
  7. pmc The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae
    D M Lyons
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:4095-106. 1996
  8. ncbi request reprint The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5
    J E Kranz
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Genes Dev 8:313-27. 1994
  9. pmc Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p
    V Cherkasova
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 151:989-1004. 1999
  10. ncbi request reprint Nuclear shuttling of yeast scaffold Ste5 is required for its recruitment to the plasma membrane and activation of the mating MAPK cascade
    S K Mahanty
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 98:501-12. 1999

Research Grants

Collaborators

  • Y Wang
  • Vera A Cherkasova
  • Maosong Qi
  • David M Simpson
  • Xiaoyan Wang
  • S K Mahanty
  • K Y Choi
  • Annette Flotho
  • Jessica Andersson
  • F W Farley
  • Mark A Sheff
  • B N Lee
  • M A Leza
  • J E Kranz
  • B Satterberg
  • Y Feng
  • D M Lyons
  • E J Goldsmith
  • K S Park
  • L Y Song
  • E Kincaid
  • M Manandhar

Detail Information

Publications27

  1. ncbi request reprint Making protein immunoprecipitates
    Elaine A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 284:1-14. 2004
    ..The Notes section gives recommendations on how to troubleshoot potential problems that can arise while doing these methodologies...
  2. pmc Ste11p MEKK signals through HOG, mating, calcineurin and PKC pathways to regulate the FKS2 gene
    Xiaoyan Wang
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    BMC Mol Biol 12:51. 2011
    ..The major signal transduction pathways that activate transcription of the FKS2 gene include the cell wall integrity and calcineurin pathways, and the Ste11p pathway...
  3. doi request reprint Analysis of mitogen-activated protein kinase activity in yeast
    Elaine A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 661:387-99. 2010
    ..Here, we describe an assay to measure Fus3 activity in immune complexes prepared from S. cerevisiae extracts. The assay conditions are applicable to other MAPKs, as well...
  4. ncbi request reprint Signal transduction. Signaling specificity in yeast
    Elaine A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Science 307:687-8. 2005
  5. ncbi request reprint The Ste5p scaffold
    E A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 114:3967-78. 2001
    ....
  6. ncbi request reprint far4, far5, and far6 define three genes required for efficient activation of MAPKs Fus3 and Kss1 and accumulation of glycogen
    V Cherkasova
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Curr Genet 40:13-26. 2001
    ..Finally, BIM1 and BIK1 have been identified as CEN suppressors of far5, suggesting that the microtubule apparatus may regulate the ability of the pheromone response pathway to promote G1 arrest...
  7. pmc The SH3-domain protein Bem1 coordinates mitogen-activated protein kinase cascade activation with cell cycle control in Saccharomyces cerevisiae
    D M Lyons
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 16:4095-106. 1996
    ..These and other results suggest that Bem1 may cross-link the Ste5-MAPK cascade complex to upstream activators and specific downstream substrates at the shmoo tip, thus enabling efficient circuitry for G1 arrest and mating...
  8. ncbi request reprint The MAP kinase Fus3 associates with and phosphorylates the upstream signaling component Ste5
    J E Kranz
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Genes Dev 8:313-27. 1994
    ..These observations suggest the possibility that Ste5 promotes signal transduction by tethering Fus3 to its activating protein kinase(s)...
  9. pmc Fus3p and Kss1p control G1 arrest in Saccharomyces cerevisiae through a balance of distinct arrest and proliferative functions that operate in parallel with Far1p
    V Cherkasova
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 151:989-1004. 1999
    ..Thus, Fus3p and Kss1p control G1 arrest through a balance of arrest functions that inhibit the Cdc28p machinery and proliferative functions that bypass this inhibition...
  10. ncbi request reprint Nuclear shuttling of yeast scaffold Ste5 is required for its recruitment to the plasma membrane and activation of the mating MAPK cascade
    S K Mahanty
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 98:501-12. 1999
    ..This novel regulatory scheme may ensure that cytoplasmic Ste5 does not activate downstream kinases in the absence of pheromone and could be applicable to other membrane-recruited signaling proteins...
  11. ncbi request reprint Functional binding between Gbeta and the LIM domain of Ste5 is required to activate the MEKK Ste11
    Y Feng
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Curr Biol 8:267-78. 1998
    ..Ste5 is thought to associate with Gbeta in a pheromone-independent manner, but it is not known if this interaction affects signaling...
  12. pmc FUS3 phosphorylates multiple components of the mating signal transduction cascade: evidence for STE12 and FAR1
    E A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Mol Biol Cell 4:495-510. 1993
    ..These data support a model in which FUS3 mediates transcription and G1 arrest by direct activation of STE12 and FAR1 and phosphorylates many other proteins involved in the response to pheromone...
  13. pmc Characterization of Fus3 localization: active Fus3 localizes in complexes of varying size and specific activity
    K Y Choi
    Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School, Boston, Massachusetts 02115 5701, USA
    Mol Biol Cell 10:1553-68. 1999
    ..Collectively, these results support Ste5's role as a tether and suggest that association of Fus3 in complexes in the presence of pheromone may prevent inactivation in addition to enhancing activation...
  14. ncbi request reprint Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae
    K Y Choi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115
    Cell 78:499-512. 1994
    ..Ste5 also increases the amount of Ste11 complexed to Ste7 and Fus3 and is required for Ste11 to function. These results substantiate a novel signal transduction component that physically links multiple kinases within a single cascade...
  15. pmc Relative dependence of different outputs of the Saccharomyces cerevisiae pheromone response pathway on the MAP kinase Fus3p
    F W Farley
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 151:1425-44. 1999
    ..Finally, suppression analysis argues that Kss1p contributes to the overall pheromone response in a wild-type strain, but that Fus3p is the critical kinase for all of the outputs tested...
  16. pmc POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2
    M A Leza
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genetics 151:531-43. 1999
    ..These and other results suggest that POG1 may regulate additional genes during vegetative growth and recovery...
  17. pmc The MAPKKK Ste11 regulates vegetative growth through a kinase cascade of shared signaling components
    B N Lee
    Department of Biological Chemistry, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:12679-84. 1999
    ..We predict that general proliferative functions may also exist for other MAPK cascades thought only to perform specialized functions...
  18. ncbi request reprint Pheromone response, mating and cell biology
    E A Elion
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Opin Microbiol 3:573-81. 2000
    ..Far1 and Cdc24 also undergo nuclear shuttling and the nuclear pool of Far1 may temporally regulate access of Cdc24 to the cell cortex...
  19. ncbi request reprint A novel functional link between MAP kinase cascades and the Ras/cAMP pathway that regulates survival
    Vera A Cherkasova
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Curr Biol 13:1220-6. 2003
    ..The conserved RasGEF Cdc25 is a likely control point, because Kss1 and Fus3 complexes associate with and phosphorylate Cdc25. Cross-regulation of Cdc25 may be a general way that MAPKs control Ras signaling networks...
  20. ncbi request reprint Formin-induced actin cables are required for polarized recruitment of the Ste5 scaffold and high level activation of MAPK Fus3
    Maosong Qi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, MA 02115, USA
    J Cell Sci 118:2837-48. 2005
    ..These findings reveal that Bni1 mediates the formation of a Ste5 scaffold/Fus3 MAPK signaling complex at polarized sites, and suggests that a pool of Ste5 may translocate along formin-induced actin cables to the cell cortex...
  21. ncbi request reprint Cdc24 regulates nuclear shuttling and recruitment of the Ste5 scaffold to a heterotrimeric G protein in Saccharomyces cerevisiae
    Yunmei Wang
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:13084-96. 2005
    ..Collectively, these findings suggest that Cdc24 mediates site-specific localization of Ste5 to a heterotrimeric G protein and may therefore ensure localized activation of the associated MAPK cascade...
  22. ncbi request reprint Localized feedback phosphorylation of Ste5p scaffold by associated MAPK cascade
    Annette Flotho
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:47391-401. 2004
    ..These findings provide evidence of a spatially regulated mechanism for post-activation control of a signaling scaffold that potentiates pathway activation...
  23. pmc Differential input by Ste5 scaffold and Msg5 phosphatase route a MAPK cascade to multiple outcomes
    Jessica Andersson
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 23:2564-76. 2004
    ..These findings reveal the versatility of scaffolds and how a single MAPK cascade mediates different outputs...
  24. ncbi request reprint MAP kinase pathways
    Maosong Qi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    J Cell Sci 118:3569-72. 2005
  25. pmc Methods for analyzing MAPK cascades
    Elaine A Elion
    Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, 240 Longwood Avenue, Building C1 302, Boston 02115 5730, USA
    Methods 40:207-8. 2006
  26. ncbi request reprint Constructing recombinant DNA molecules by PCR
    Elaine A Elion
    Harvard Medical School, Boston, Massachusetts, USA
    Curr Protoc Mol Biol . 2007
    ....
  27. pmc Nuclear export and plasma membrane recruitment of the Ste5 scaffold are coordinated with oligomerization and association with signal transduction components
    Yunmei Wang
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Biol Cell 14:2543-58. 2003
    ....

Research Grants17

  1. CELL CYCLE ARREST AND SIGNAL TRANSDUCTION IN YEAST
    ELAINE ELION; Fiscal Year: 2004
    ..abstract_text> ..
  2. CELL CYCLE ARREST AND SIGNAL TRANSDUCTION IN YEAST
    ELAINE ELION; Fiscal Year: 2000
    ..4. The role of Ste5 in mediating pathway specificity will be investigated by isolating and analyzing mutant forms of Ste5 and Ste11 that activate other MAP kinase cascades. ..
  3. CELL CYCLE ARREST AND SIGNAL TRANSDUCTION IN YEAST
    ELAINE ELION; Fiscal Year: 1993
    ..3) To identify proteins which either interact with FUS3 or are its functional homologues by genetic screens...
  4. CELL CYCLE ARREST AND SIGNAL TRANSDUCTION IN YEAST
    ELAINE ELION; Fiscal Year: 2009
    ..abstract_text> ..