KEVIN C EGGAN

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Dolly's legacy: human nuclear transplantation and better medicines for our children
    Kevin Eggan
    The Stowers Medical Institute, Harvard Stem Cell Institute and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cloning Stem Cells 9:21-5. 2007
  2. pmc Recipient cell nuclear factors are required for reprogramming by nuclear transfer
    Dieter Egli
    The Howard Hughes Medical Institute, Stowers Medical Institute, Harvard Stem Cell Institute and Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Development 137:1953-63. 2010
  3. doi request reprint Using stem cells and reprogramming to understand disease
    Kevin Eggan
    Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Regen Med 3:799-801. 2008
  4. doi request reprint Short-circuiting epiblast development
    Kevin Eggan
    The Stowers Medical Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Cell Stem Cell 1:131-2. 2007
  5. pmc Progress toward the clinical application of patient-specific pluripotent stem cells
    Evangelos Kiskinis
    The Stowers Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    J Clin Invest 120:51-9. 2010
  6. pmc Non-cell autonomous effect of glia on motor neurons in an embryonic stem cell-based ALS model
    Francesco Paolo Di Giorgio
    The Stowers Medical Institute, the Harvard Stem Cell Institute Harvard University, 7 Divinity Ave, Cambridge, Massachusetts 02138, USA
    Nat Neurosci 10:608-14. 2007
  7. ncbi request reprint Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells
    Chad A Cowan
    Howard Hughes Medical Institute, Harvard Stem Cell Institute, Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Science 309:1369-73. 2005
  8. ncbi request reprint Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei
    Alex Bortvin
    Howard Hughes Medical Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
    Development 130:1673-80. 2003
  9. doi request reprint Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation
    Francesco Paolo Di Giorgio
    The Harvard Stem Cell Institute, The Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Cambridge, MA 02138, USA
    Cell Stem Cell 3:637-48. 2008
  10. pmc Reprogramming after chromosome transfer into mouse blastomeres
    Dieter Egli
    Stowers Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02140, USA
    Curr Biol 19:1403-9. 2009

Research Grants

Collaborators

Detail Information

Publications28

  1. ncbi request reprint Dolly's legacy: human nuclear transplantation and better medicines for our children
    Kevin Eggan
    The Stowers Medical Institute, Harvard Stem Cell Institute and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Cloning Stem Cells 9:21-5. 2007
  2. pmc Recipient cell nuclear factors are required for reprogramming by nuclear transfer
    Dieter Egli
    The Howard Hughes Medical Institute, Stowers Medical Institute, Harvard Stem Cell Institute and Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Development 137:1953-63. 2010
    ..Our findings demonstrate the remarkable flexibility of the reprogramming process and support the importance of nuclear transcriptional regulators in mediating reprogramming...
  3. doi request reprint Using stem cells and reprogramming to understand disease
    Kevin Eggan
    Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Regen Med 3:799-801. 2008
    ..His accomplishments include cloning mice from olfactory sensory neurons, deriving embryonic germ cells and male gametes from embryonic stem cells and characterizing the abnormalities that sometimes arise as a result of nuclear transfer...
  4. doi request reprint Short-circuiting epiblast development
    Kevin Eggan
    The Stowers Medical Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Cell Stem Cell 1:131-2. 2007
    ..Two recent papers describe the derivation of stem cell lines from mouse epiblast that may provide an explanation for the unique character of human embryonic stem cells...
  5. pmc Progress toward the clinical application of patient-specific pluripotent stem cells
    Evangelos Kiskinis
    The Stowers Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    J Clin Invest 120:51-9. 2010
    ....
  6. pmc Non-cell autonomous effect of glia on motor neurons in an embryonic stem cell-based ALS model
    Francesco Paolo Di Giorgio
    The Stowers Medical Institute, the Harvard Stem Cell Institute Harvard University, 7 Divinity Ave, Cambridge, Massachusetts 02138, USA
    Nat Neurosci 10:608-14. 2007
    ..These phenotypes displayed in culture could provide cell-based assays for the identification of new ALS drugs...
  7. ncbi request reprint Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells
    Chad A Cowan
    Howard Hughes Medical Institute, Harvard Stem Cell Institute, Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Science 309:1369-73. 2005
    ..These results establish that hES cells can reprogram the transcriptional state of somatic nuclei and provide a system for investigating the underlying mechanisms...
  8. ncbi request reprint Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nuclei
    Alex Bortvin
    Howard Hughes Medical Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
    Development 130:1673-80. 2003
    ..These observations suggest that failure to reactivate the full spectrum of these Oct4-related genes may contribute to embryonic lethality in somatic-cell clones...
  9. doi request reprint Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation
    Francesco Paolo Di Giorgio
    The Harvard Stem Cell Institute, The Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Cambridge, MA 02138, USA
    Cell Stem Cell 3:637-48. 2008
    ..Our findings demonstrate the relevance of these non-cell-autonomous effects to human motor neurons and more broadly demonstrate the utility of human embryonic stem cells for studying disease and identifying potential therapeutics...
  10. pmc Reprogramming after chromosome transfer into mouse blastomeres
    Dieter Egli
    Stowers Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02140, USA
    Curr Biol 19:1403-9. 2009
    ....
  11. doi request reprint Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons
    John T Dimos
    Harvard Stem Cell Institute, Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Science 321:1218-21. 2008
    ..These patient-specific iPS cells possess properties of embryonic stem cells and were successfully directed to differentiate into motor neurons, the cell type destroyed in ALS...
  12. doi request reprint Mediators of reprogramming: transcription factors and transitions through mitosis
    Dieter Egli
    The Stowers Medical Institute, Harvard Stem Cell Institute and the Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA
    Nat Rev Mol Cell Biol 9:505-16. 2008
    ....
  13. ncbi request reprint Nuclear cloning, stem cells, and genomic reprogramming
    Rudolf Jeanisch
    Whitehead Institute, Cambridge, Massachusetts 02142, USA
    Cloning Stem Cells 4:389-96. 2002
    ..In contrast to reproductive cloning, faulty reprogramming of the donor nucleus does not tend to interfere with the application of nuclear transfer technology for therapeutic purposes (therapeutic cloning)...
  14. ncbi request reprint Nuclear cloning, epigenetic reprogramming and cellular differentiation
    Rudolf Jaenisch
    Whitehead Institute, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Novartis Found Symp 265:107-18; discussion 118-28. 2005
    ..Finally, we discuss our recent studies on the reprogramming of nuclei from terminally differentiated neurons and from cancer cells...
  15. ncbi request reprint A transcriptional logic for nuclear reprogramming
    Kit T Rodolfa
    The Stowers Medical Institute, Harvard Stem Cell Institute, Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
    Cell 126:652-5. 2006
    ..In this issue of Cell, Takahashi and Yamanaka (2006) take a rational approach to identifying a suite of embryonic transcription factors whose overexpression restores pluripotency to adult somatic cells...
  16. ncbi request reprint Derivation of embryonic germ cells and male gametes from embryonic stem cells
    Niels Geijsen
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 427:148-54. 2004
    ..Our ability to derive germ cells from embryonic stem cells provides an accessible in vitro model system for studies of germline epigenetic modification and mammalian gametogenesis...
  17. doi request reprint Shushing down the epigenetic landscape towards stem cell differentiation
    Justin K Ichida
    The Howard Hughes Medical Institute, Stowers Medical Institute, Harvard Stem Cell Institute and the Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
    Development 137:2455-60. 2010
    ....
  18. ncbi request reprint Mice cloned from olfactory sensory neurons
    Kevin Eggan
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 428:44-9. 2004
    ....
  19. ncbi request reprint Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementation
    Kevin Eggan
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Nat Biotechnol 20:455-9. 2002
    ..Our data also indicate that ES cells have inherently unstable karyotypes, but this instability does not interfere with production of adult ES cell tetraploid mice...
  20. ncbi request reprint Micromanipulating dosage compensation: understanding X-chromosome inactivation through nuclear transplantation
    Kevin Eggan
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Semin Cell Dev Biol 14:349-58. 2003
    ..These results suggest epigenetic information established during embryonic X-inactivation is functionally equivalent to the gametic imprint...
  21. pmc Abnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nuclei
    David Humpherys
    Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 99:12889-94. 2002
    ..Our results demonstrate frequent abnormal gene expression in clones, in which most expression abnormalities appear common to the NT procedure whereas others appear to reflect the particular donor nucleus...
  22. ncbi request reprint Ovulated oocytes in adult mice derive from non-circulating germ cells
    Kevin Eggan
    The Stowers Medical Institute, Harvard Stem Cell Institute and The Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 441:1109-14. 2006
    ..Instead, cells that travelled to the ovary through the bloodstream exhibited properties characteristic of committed blood leukocytes...
  23. pmc Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal
    Kathy K Niakan
    Stowers Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA
    Genes Dev 24:312-26. 2010
    ..By elaborating the function of Sox17, our results provide insight into how the transcriptional network promoting ES cell self-renewal is interrupted, allowing cellular differentiation...
  24. pmc Dnmt1 overexpression causes genomic hypermethylation, loss of imprinting, and embryonic lethality
    Detlev Biniszkiewicz
    Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
    Mol Cell Biol 22:2124-35. 2002
    ..Injection of Dnmt1-overexpressing embryonic stem cells in diploid or tetraploid blastocysts resulted in lethality of the embryo, which resembled embryonic lethality caused by Dnmt1 deficiency...
  25. ncbi request reprint Developmental reprogramming after chromosome transfer into mitotic mouse zygotes
    Dieter Egli
    The Stowers Medical Institute, Harvard Stem Cell Institute and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Nature 447:679-85. 2007
    ..Thus, human zygotes and perhaps human embryonic blastomeres may be useful supplements to human oocytes for the creation of patient-derived human embryonic stem cells...
  26. ncbi request reprint Differentiation of F1 embryonic stem cells into viable male and female mice by tetraploid embryo complementation
    Kevin Eggan
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Methods Enzymol 365:25-39. 2003
  27. ncbi request reprint Flp recombinase regulated lacZ expression at the ROSA26 locus
    Richard Possemato
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Genesis 32:184-6. 2002

Research Grants6

  1. Developmental Reprogramming after Nuclear Transfer
    KEVIN C EGGAN; Fiscal Year: 2010
    ..abstract_text> ..