Research Topics
| KEVIN C EGGANSummaryAffiliation: Harvard University Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Using stem cells and reprogramming to understand diseaseKevin Eggan
Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, 7 Divinity Avenue, Cambridge, MA 02138, USA
Regen Med 3:799-801. 2008..His accomplishments include cloning mice from olfactory sensory neurons, deriving embryonic germ cells and male gametes from embryonic stem cells and characterizing the abnormalities that sometimes arise as a result of nuclear transfer...
Recipient cell nuclear factors are required for reprogramming by nuclear transferDieter Egli
The Howard Hughes Medical Institute, Stowers Medical Institute, Harvard Stem Cell Institute and Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
Development 137:1953-63. 2010..Our findings demonstrate the remarkable flexibility of the reprogramming process and support the importance of nuclear transcriptional regulators in mediating reprogramming...
Dolly's legacy: human nuclear transplantation and better medicines for our childrenKevin Eggan
The Stowers Medical Institute, Harvard Stem Cell Institute and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Cloning Stem Cells 9:21-5. 2007- Short-circuiting epiblast developmentKevin Eggan
The Stowers Medical Institute, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
Cell Stem Cell 1:131-2. 2007..Two recent papers describe the derivation of stem cell lines from mouse epiblast that may provide an explanation for the unique character of human embryonic stem cells... - Progress toward the clinical application of patient-specific pluripotent stem cellsEvangelos Kiskinis
The Stowers Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
J Clin Invest 120:51-9. 2010.... - Nuclear reprogramming of somatic cells after fusion with human embryonic stem cellsChad A Cowan
Howard Hughes Medical Institute, Harvard Stem Cell Institute, Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
Science 309:1369-73. 2005..These results establish that hES cells can reprogram the transcriptional state of somatic nuclei and provide a system for investigating the underlying mechanisms... - Non-cell autonomous effect of glia on motor neurons in an embryonic stem cell-based ALS modelFrancesco Paolo Di Giorgio
The Stowers Medical Institute, the Harvard Stem Cell Institute Harvard University, 7 Divinity Ave, Cambridge, Massachusetts 02138, USA
Nat Neurosci 10:608-14. 2007..These phenotypes displayed in culture could provide cell-based assays for the identification of new ALS drugs... - Incomplete reactivation of Oct4-related genes in mouse embryos cloned from somatic nucleiAlex Bortvin
Howard Hughes Medical Institute, 9 Cambridge Center, Cambridge, MA 02142, USA
Development 130:1673-80. 2003..These observations suggest that failure to reactivate the full spectrum of these Oct4-related genes may contribute to embryonic lethality in somatic-cell clones... - Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutationFrancesco Paolo Di Giorgio
The Harvard Stem Cell Institute, The Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Cambridge, MA 02138, USA
Cell Stem Cell 3:637-48. 2008..Our findings demonstrate the relevance of these non-cell-autonomous effects to human motor neurons and more broadly demonstrate the utility of human embryonic stem cells for studying disease and identifying potential therapeutics... - Reprogramming after chromosome transfer into mouse blastomeresDieter Egli
Stowers Medical Institute, Harvard Stem Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02140, USA
Curr Biol 19:1403-9. 2009.... - Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neuronsJohn T Dimos
Harvard Stem Cell Institute, Stowers Medical Institute, Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
Science 321:1218-21. 2008..These patient-specific iPS cells possess properties of embryonic stem cells and were successfully directed to differentiate into motor neurons, the cell type destroyed in ALS... - Nuclear cloning, epigenetic reprogramming and cellular differentiationRudolf Jaenisch
Whitehead Institute, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
Novartis Found Symp 265:107-18; discussion 118-28. 2005..Finally, we discuss our recent studies on the reprogramming of nuclei from terminally differentiated neurons and from cancer cells... - Nuclear cloning, stem cells, and genomic reprogrammingRudolf Jeanisch
Whitehead Institute, Cambridge, Massachusetts 02142, USA
Cloning Stem Cells 4:389-96. 2002..In contrast to reproductive cloning, faulty reprogramming of the donor nucleus does not tend to interfere with the application of nuclear transfer technology for therapeutic purposes (therapeutic cloning)... - Mediators of reprogramming: transcription factors and transitions through mitosisDieter Egli
The Stowers Medical Institute, Harvard Stem Cell Institute and the Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA
Nat Rev Mol Cell Biol 9:505-16. 2008.... - Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewalKathy K Niakan
Stowers Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA
Genes Dev 24:312-26. 2010..By elaborating the function of Sox17, our results provide insight into how the transcriptional network promoting ES cell self-renewal is interrupted, allowing cellular differentiation... - Shushing down the epigenetic landscape towards stem cell differentiationJustin K Ichida
The Howard Hughes Medical Institute, Stowers Medical Institute, Harvard Stem Cell Institute and the Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA
Development 137:2455-60. 2010.... - A transcriptional logic for nuclear reprogrammingKit T Rodolfa
The Stowers Medical Institute, Harvard Stem Cell Institute, Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, MA 02138, USA
Cell 126:652-5. 2006..In this issue of Cell, Takahashi and Yamanaka (2006) take a rational approach to identifying a suite of embryonic transcription factors whose overexpression restores pluripotency to adult somatic cells... - Ovulated oocytes in adult mice derive from non-circulating germ cellsKevin Eggan
The Stowers Medical Institute, Harvard Stem Cell Institute and The Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 441:1109-14. 2006..Instead, cells that travelled to the ovary through the bloodstream exhibited properties characteristic of committed blood leukocytes... - Micromanipulating dosage compensation: understanding X-chromosome inactivation through nuclear transplantationKevin Eggan
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
Semin Cell Dev Biol 14:349-58. 2003..These results suggest epigenetic information established during embryonic X-inactivation is functionally equivalent to the gametic imprint... - Mice cloned from olfactory sensory neuronsKevin Eggan
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Nature 428:44-9. 2004.... - Derivation of embryonic germ cells and male gametes from embryonic stem cellsNiels Geijsen
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Nature 427:148-54. 2004..Our ability to derive germ cells from embryonic stem cells provides an accessible in vitro model system for studies of germline epigenetic modification and mammalian gametogenesis... - Abnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nucleiDavid Humpherys
Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
Proc Natl Acad Sci U S A 99:12889-94. 2002..Our results demonstrate frequent abnormal gene expression in clones, in which most expression abnormalities appear common to the NT procedure whereas others appear to reflect the particular donor nucleus... - Male and female mice derived from the same embryonic stem cell clone by tetraploid embryo complementationKevin Eggan
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
Nat Biotechnol 20:455-9. 2002..Our data also indicate that ES cells have inherently unstable karyotypes, but this instability does not interfere with production of adult ES cell tetraploid mice... - Developmental reprogramming after chromosome transfer into mitotic mouse zygotesDieter Egli
The Stowers Medical Institute, Harvard Stem Cell Institute and Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Nature 447:679-85. 2007..Thus, human zygotes and perhaps human embryonic blastomeres may be useful supplements to human oocytes for the creation of patient-derived human embryonic stem cells... - Dnmt1 overexpression causes genomic hypermethylation, loss of imprinting, and embryonic lethalityDetlev Biniszkiewicz
Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, Massachusetts 02142, USA
Mol Cell Biol 22:2124-35. 2002..Injection of Dnmt1-overexpressing embryonic stem cells in diploid or tetraploid blastocysts resulted in lethality of the embryo, which resembled embryonic lethality caused by Dnmt1 deficiency... - Differentiation of F1 embryonic stem cells into viable male and female mice by tetraploid embryo complementationKevin Eggan
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Methods Enzymol 365:25-39. 2003 - Flp recombinase regulated lacZ expression at the ROSA26 locusRichard Possemato
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Genesis 32:184-6. 2002
Research Grants
- Developmental Reprogramming after Nuclear TransferKEVIN C EGGAN; Fiscal Year: 2010..abstract_text> ..