Nicholas Dyson

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi The regulation of E2F by pRB-family proteins
    N Dyson
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129 USA
    Genes Dev 12:2245-62. 1998
  2. pmc A kinase shRNA screen links LATS2 and the pRB tumor suppressor
    Katrin Tschop
    Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Genes Dev 25:814-30. 2011
  3. pmc Requirements for dE2F function in proliferating cells and in post-mitotic differentiating cells
    A Brook
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    EMBO J 15:3676-83. 1996
  4. ncbi Targeted disruption of p107: functional overlap between p107 and Rb
    M H Lee
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA
    Genes Dev 10:1621-32. 1996
  5. pmc Histone deacetylase-dependent transcriptional repression by pRB in yeast occurs independently of interaction through the LXCXE binding cleft
    B K Kennedy
    Massachusetts General Hospital Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 98:8720-5. 2001
  6. pmc Nuclear organization of DNA replication in primary mammalian cells
    B K Kennedy
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA
    Genes Dev 14:2855-68. 2000
  7. pmc pRB plays an essential role in cell cycle arrest induced by DNA damage
    E A Harrington
    Laboratory of Molecular Oncology, MGH Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 95:11945-50. 1998
  8. pmc Mutation of Drosophila Lsd1 disrupts H3-K4 methylation, resulting in tissue-specific defects during development
    Luisa Di Stefano
    Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Curr Biol 17:808-12. 2007
  9. pmc Distinct mechanisms of E2F regulation by Drosophila RBF1 and RBF2
    Olivier Stevaux
    Massachusetts General Hospital Cancer Center, Laboratory of Molecular Oncology, Charlestown, MA 02129, USA
    EMBO J 21:4927-37. 2002
  10. pmc Nuclear reorganization of mammalian DNA synthesis prior to cell cycle exit
    David A Barbie
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Mol Cell Biol 24:595-607. 2004

Research Grants

  1. The E2F/RBF network
    Nicholas Dyson; Fiscal Year: 2006
  2. MUTAGENESIS OF THE PRB POCKET DOMAIN
    Nicholas Dyson; Fiscal Year: 2004
  3. E2F-dependent cell proliferation
    Nicholas Dyson; Fiscal Year: 2007
  4. Mutagenesis of the pRB pocket
    Nicholas Dyson; Fiscal Year: 2009
  5. The Enhancement and Suppression of E2F-Dependent Apoptosis
    Nicholas J Dyson; Fiscal Year: 2010
  6. CONTROL OF CELL PROLIFERATION BY RB-FAMILY PROTEINS
    Nicholas Dyson; Fiscal Year: 1999
  7. E2F-dependent cell proliferation
    Nicholas J Dyson; Fiscal Year: 2010

Collaborators

  • Benjamin B Andken
  • B K Kennedy
  • Jun Yuan Ji
  • Larisa Litovchick
  • Jiyong Zhao
  • W Du
  • M Vidal
  • Mong Hong Lee
  • E A Harrington
  • Lea Harrington
  • L Yamasaki
  • Michael Rape
  • William Kaelin
  • Marie K Classon
  • Nam Sung Moon
  • Maxim V Frolov
  • Olivier Stevaux
  • Erick J Morris
  • Luisa Di Stefano
  • Ulrich K BinnĂ©
  • Frederick A Dick
  • Anabel Herr
  • Ed Harlow
  • Katrin Tschop
  • Dessislava K Dimova
  • Barbie Taylor-Harding
  • Sander van den Heuvel
  • Michelle S Longworth
  • Anders M Naar
  • Christian E Isaac
  • Eun Jeong Kwon
  • Desssislava K Dimova
  • David A Barbie
  • Matthew J Kohn
  • Dessislava Dimova
  • Jeffrey Settleman
  • James A DeCaprio
  • Andrew R Conery
  • Kevin M Haigis
  • Fajun Yang
  • Kristin White
  • Reena Patel
  • Wenyi Wei
  • James W Rocco
  • Natalie Erdmann
  • Nathalie G Berube
  • William A Michaud
  • Laurie A Seifried
  • Sarah M Francis
  • Alison L Martens
  • Piotr Sicinski
  • Lisa M Julian
  • Brett R Johnson
  • Brian A Kudlow
  • Michael Korenjak
  • Richard Frock
  • Alexander Brehm
  • Roderick T Bronson
  • A Brook
  • Erick Morris
  • J E Xie

Detail Information

Publications28

  1. ncbi The regulation of E2F by pRB-family proteins
    N Dyson
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129 USA
    Genes Dev 12:2245-62. 1998
  2. pmc A kinase shRNA screen links LATS2 and the pRB tumor suppressor
    Katrin Tschop
    Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Genes Dev 25:814-30. 2011
    ..Our results reveal a functional connection between the pRB and Hippo tumor suppressor pathways, and suggest that low levels of LATS2 may undermine the ability of pRB to induce a permanent cell cycle arrest in tumor cells...
  3. pmc Requirements for dE2F function in proliferating cells and in post-mitotic differentiating cells
    A Brook
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    EMBO J 15:3676-83. 1996
    ..These results show that dE2F is required at multiple stages of development and suggest that E2F may have an important function in post-mitotic cells in addition to its role during cell proliferation...
  4. ncbi Targeted disruption of p107: functional overlap between p107 and Rb
    M H Lee
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA
    Genes Dev 10:1621-32. 1996
    ..These results provide the first in vivo evidence that p107 and Rb have overlapping functions in some tissues of the developing and adult mouse...
  5. pmc Histone deacetylase-dependent transcriptional repression by pRB in yeast occurs independently of interaction through the LXCXE binding cleft
    B K Kennedy
    Massachusetts General Hospital Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 98:8720-5. 2001
    ..By comparing the genetic requirements of DB-pRB repression in yeast to those of other DB-repressor fusions, we can suggest a mechanism by which pRB recruits histone deacetylase activity...
  6. pmc Nuclear organization of DNA replication in primary mammalian cells
    B K Kennedy
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA
    Genes Dev 14:2855-68. 2000
    ..These findings indicate that in normal mammalian cells, the onset of DNA synthesis is coordinately regulated at a small number of previously unrecognized perinucleolar sites that are selected in early G(1)-phase...
  7. pmc pRB plays an essential role in cell cycle arrest induced by DNA damage
    E A Harrington
    Laboratory of Molecular Oncology, MGH Cancer Center, Building 149, 13th Street, Charlestown, MA 02129, USA
    Proc Natl Acad Sci U S A 95:11945-50. 1998
    ..Furthermore, because many cancer therapies act by damaging DNA, these findings also have implications for the treatment of tumors in which pRB is inactivated...
  8. pmc Mutation of Drosophila Lsd1 disrupts H3-K4 methylation, resulting in tissue-specific defects during development
    Luisa Di Stefano
    Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Curr Biol 17:808-12. 2007
    ..Taken together, these results show that dLsd1-mediated H3-K4 demethylation has a significant and specific role in Drosophila development...
  9. pmc Distinct mechanisms of E2F regulation by Drosophila RBF1 and RBF2
    Olivier Stevaux
    Massachusetts General Hospital Cancer Center, Laboratory of Molecular Oncology, Charlestown, MA 02129, USA
    EMBO J 21:4927-37. 2002
    ..These results suggest that there is a remarkable degree of symmetry in the arrangement of E2F and RB family members in mammalian cells and in DROSOPHILA...
  10. pmc Nuclear reorganization of mammalian DNA synthesis prior to cell cycle exit
    David A Barbie
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Mol Cell Biol 24:595-607. 2004
    ..These results suggest that mammalian cells undergo a large-scale reorganization of chromatin during the rounds of DNA replication that precede cell cycle exit...
  11. pmc Adenovirus E1A makes two distinct contacts with the retinoblastoma protein
    N Dyson
    Massachusetts General Hospital Cancer Center, Charlestown, 02129
    J Virol 66:4606-11. 1992
    ..These data support the notion that the pRB-binding domain of E1A contains at least two functional elements...
  12. ncbi Molecular mechanisms of E2F-dependent activation and pRB-mediated repression
    Maxim V Frolov
    Massachusetts General Hospital Cancer Center, Bldg 149, 13th Street, Charlestown, MA 02129, USA
    J Cell Sci 117:2173-81. 2004
    ..Such repression is evident in both actively proliferating cells and in cells that have withdrawn from the cell cycle...
  13. ncbi The E2F transcriptional network: old acquaintances with new faces
    Desssislava K Dimova
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Oncogene 24:2810-26. 2005
    ..In this review, we will discuss these recent developments and describe how they are beginning to shape a new and revised picture of the E2F transcriptional program...
  14. pmc dDP is needed for normal cell proliferation
    Maxim V Frolov
    Massachusetts General Hospital Cancer Center, Bldg 149, 13th St, Charlestown, MA 02129, USA
    Mol Cell Biol 25:3027-39. 2005
    ..Thus, dDP is not essential for developmental control of the G1-to-S transition, but it is required for normal cell proliferation, for optimal DNA synthesis, and for efficient G2/M progression...
  15. pmc p55, the Drosophila ortholog of RbAp46/RbAp48, is required for the repression of dE2F2/RBF-regulated genes
    Barbie Taylor-Harding
    Massachusetts General Hospital, Center for Cancer Research, Building 149, 13th St, Charlestown, MA 02129, USA
    Mol Cell Biol 24:9124-36. 2004
    ....
  16. ncbi A revised picture of the E2F transcriptional network and RB function
    Olivier Stevaux
    Massachusetts General Hospital Cancer Center, Laboratory of Molecular Oncology, Charlestown, MA 02129, USA
    Curr Opin Cell Biol 14:684-91. 2002
    ..One thorny issue remains: do our current molecular models of E2F and retinoblastoma action explain all of the functions of these proteins in vivo?..
  17. ncbi Dp1 is required for extra-embryonic development
    Matthew J Kohn
    Department of Biological Sciences, Columbia University, New York, NY 10027 USA
    Development 130:1295-305. 2003
    ..Thus, DP1 is absolutely required for extra-embryonic development and consequently embryonic survival, consistent with E2F/DP1 normally acting to promote growth in vivo...
  18. pmc E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8
    Erick J Morris
    Laboratory of Molecular Oncology, Massachusetts General Hospital Cancer Center and Harvard Medical School, 13th Street, Building 149, Charlestown, Massachusetts 02129, USA
    Nature 455:552-6. 2008
    ..Thus, by retaining RB1 and amplifying CDK8, colorectal tumour cells select conditions that collectively suppress E2F1 and enhance the activity of beta-catenin...
  19. doi Conserved functions of the pRB and E2F families
    Sander van den Heuvel
    Developmental Biology, Utrecht University, Utrecht, The Netherlands
    Nat Rev Mol Cell Biol 9:713-24. 2008
    ....
  20. pmc E2F and p53 induce apoptosis independently during Drosophila development but intersect in the context of DNA damage
    Nam Sung Moon
    Massachusetts General Hospital Cancer Research Center, Charlestown, Massachusetts, United States of America
    PLoS Genet 4:e1000153. 2008
    ....
  21. pmc RBF1 promotes chromatin condensation through a conserved interaction with the Condensin II protein dCAP-D3
    Michelle S Longworth
    Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Genes Dev 22:1011-24. 2008
    ..These results uncover an unexpected link between pRB/RBF1 and chromatin condensation, providing a mechanism by which the functional inactivation of RB family members in human tumor cells may contribute to genome instability...
  22. pmc Three regions of the pRB pocket domain affect its inactivation by human papillomavirus E7 proteins
    Frederick A Dick
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA
    J Virol 76:6224-34. 2002
    ..These residues most likely form ionic interactions with conserved acidic amino acids on E7 since a stable pRB/E7 interaction was restored when the lysine residues on pRB and the acidic residues on E7 were interchanged...
  23. ncbi Retinoblastoma protein and anaphase-promoting complex physically interact and functionally cooperate during cell-cycle exit
    Ulrich K Binné
    Massachusetts General Hospital Cancer Center and Harvard Medical School, Charlestown, Massachusetts, MA 02129, USA
    Nat Cell Biol 9:225-32. 2007
    ..The results reveal an unexpected physical convergence between the pRB tumour-suppressor protein and E3 ligase complexes, and raise the possibility that pRB may direct APC/C to additional targets during pRB-mediated cell-cycle exit...
  24. pmc Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo
    Erick J Morris
    Massachusetts General Hospital Cancer Center, Laboratory of Molecular Oncology, Charlestown, Massachusetts, United States of America
    PLoS Genet 2:e196. 2006
    ..Therefore, inhibition of Api5 function might offer a possible mechanism for antitumor exploitation...
  25. pmc The retinoblastoma protein regulates pericentric heterochromatin
    Christian E Isaac
    Cancer Research Labs, 790 Commissioners Road East, London, Ontario, Canada, N6A 4L6
    Mol Cell Biol 26:3659-71. 2006
    ..These results demonstrate the surprising finding that pRb uses the LXCXE binding cleft to control chromatin structure for the regulation of events beyond the G(1)-to-S-phase transition...
  26. ncbi Retinoblastoma family 2 is required in vivo for the tissue-specific repression of dE2F2 target genes
    Olivier Stevaux
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts 02129, USA
    Cell Cycle 4:1272-80. 2005
    ..These results demonstrate that RBF2 has a unique function in repressing E2F-regulated differentiation markers and that dE2F2 and RBF2 are required to regulate different sets of target genes in different tissues...
  27. pmc Cell cycle-dependent and cell cycle-independent control of transcription by the Drosophila E2F/RB pathway
    Dessislava K Dimova
    Massachusetts General Hospital Cancer Center, Charlestown, Massachusetts, 02129 USA
    Genes Dev 17:2308-20. 2003
    ..As a result, dE2F/RBF regulation is used to link gene expression with cell cycle progression at some targets while simultaneously providing stable repression at others...
  28. pmc G1 cyclin-dependent kinases are insufficient to reverse dE2F2-mediated repression
    Maxim V Frolov
    Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA
    Genes Dev 17:723-8. 2003
    ..The implication of these results is that cells containing dE2F2 require dE2F1 to either prevent, or reverse, dE2F-mediated repression...

Research Grants34

  1. The E2F/RBF network
    Nicholas Dyson; Fiscal Year: 2006
    ..In the previous period we discovered that cells homozygous mutant for both de2fl and de2f2 can proliferate. Aim 3 will use E2F- or DP-deficient cells to determine which aspects of cell cycle regulation require E2F activity. ..
  2. MUTAGENESIS OF THE PRB POCKET DOMAIN
    Nicholas Dyson; Fiscal Year: 2004
    ..The LXCXE-binding cleft is maintained in all pRB-homologs that have been identified, suggesting that it is critical for a conserved function of pRB. ..
  3. E2F-dependent cell proliferation
    Nicholas Dyson; Fiscal Year: 2007
    ..Starting from the list of genes isolated in the screen we plan to identify and characterize proteins that limit E2F-dependent proliferation, and proteins that are needed for the activation of dE2F1-dependent transcription. ..
  4. Mutagenesis of the pRB pocket
    Nicholas Dyson; Fiscal Year: 2009
    ..By examining cells as they respond to different types of cell cycle arrest signals we will also discover whether pRB recruits different types of repressor complexes in different contexts. ..
  5. The Enhancement and Suppression of E2F-Dependent Apoptosis
    Nicholas J Dyson; Fiscal Year: 2010
    ..abstract_text> ..
  6. CONTROL OF CELL PROLIFERATION BY RB-FAMILY PROTEINS
    Nicholas Dyson; Fiscal Year: 1999
    ..3) identify and characterize a novel regulator E2F that is specifically upregulated in cells lacking both p1O7 and p130. ..
  7. E2F-dependent cell proliferation
    Nicholas J Dyson; Fiscal Year: 2010
    ..Starting from the list of genes isolated in the screen we plan to identify and characterize proteins that limit E2F-dependent proliferation, and proteins that are needed for the activation of dE2F1-dependent transcription. ..