Ronald Desrosiers

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Vaccine protection against simian immunodeficiency virus in monkeys using recombinant gamma-2 herpesvirus
    John P Bilello
    New England Primate Research Center, Harvard Medical School, Southborough, MA 01772 9102, USA
    J Virol 85:12708-20. 2011
  2. pmc Evidence against extracellular exposure of a highly immunogenic region in the C-terminal domain of the simian immunodeficiency virus gp41 transmembrane protein
    Thomas S Postler
    New England Primate Research Center, Department of Microbiology and Immunobiology, Harvard Medical School, Southborough, Massachusetts, USA
    J Virol 86:1145-57. 2012
  3. ncbi request reprint Prospects for an AIDS vaccine
    Ronald C Desrosiers
    New England Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    Nat Med 10:221-3. 2004
  4. pmc Impact of Nef-mediated downregulation of major histocompatibility complex class I on immune response to simian immunodeficiency virus
    Tomek Swigut
    New England Primate Research Center, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 78:13335-44. 2004
  5. ncbi request reprint Emergence of cytotoxic T lymphocyte escape mutations in nonpathogenic simian immunodeficiency virus infection
    A Kaur
    Division of Immunology, New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772, USA
    Eur J Immunol 31:3207-17. 2001
  6. pmc Identification of highly attenuated mutants of simian immunodeficiency virus
    R C Desrosiers
    New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    J Virol 72:1431-7. 1998
  7. pmc Induction of vigorous cytotoxic T-lymphocyte responses by live attenuated simian immunodeficiency virus
    R P Johnson
    Division of Immunology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA
    J Virol 71:7711-8. 1997
  8. ncbi request reprint Protective immunity induced by live attenuated simian immunodeficiency virus
    R P Johnson
    Division of Immunology, New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772, USA
    Curr Opin Immunol 10:436-43. 1998
  9. pmc Glycosylation of gp41 of simian immunodeficiency virus shields epitopes that can be targets for neutralizing antibodies
    Eloisa Yuste
    Department of Microbiology and Molecular Genetics, Harvard Medical School, New England Primate Research Center, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 82:12472-86. 2008
  10. ncbi request reprint Construction and in vitro properties of SIVmac mutants with deletions in "nonessential" genes
    J S Gibbs
    Harvard Medical School, New England Regional Primate Research Center, Southborough, Massachusetts 01772 9102
    AIDS Res Hum Retroviruses 10:607-16. 1994

Research Grants

  1. MOLECULAR BASIS FOR SIV PATHOGENICITY
    Ronald Desrosiers; Fiscal Year: 2007
  2. Rhesus Monkey Rhadinovirus Glycoproteins, Entry, and Neutralization
    Ronald Desrosiers; Fiscal Year: 2009
  3. MOLECULAR BASIS FOR SIV PATHOGENICITY
    Ronald Desrosiers; Fiscal Year: 2009
  4. Gamma-2 Herpesviruses as Vaccine Vectors for AIDS
    Ronald Desrosiers; Fiscal Year: 2009
  5. Rhesus Monkey Rhadinovirus Glycoproteins, Entry, and Neutralization
    Ronald C Desrosiers; Fiscal Year: 2010
  6. LIVE ATTENUATED MULTIPLY DELETED HIV1 VACCINE FOR AIDS
    Ronald Desrosiers; Fiscal Year: 2004
  7. Contributions of HIV qp41 TM Protein to Pathogenesis
    Ronald Desrosiers; Fiscal Year: 2005
  8. MOLECULAR BASIS FOR SIV PATHOGENICITY
    Ronald Desrosiers; Fiscal Year: 1993
  9. 19th Annual Symposium-Nonhuman Primate Models for AIDS
    Ronald Desrosiers; Fiscal Year: 2001
  10. MOLECULAR BASIS FOR SIV PATHOGENICITY
    Ronald C Desrosiers; Fiscal Year: 2010

Detail Information

Publications66

  1. pmc Vaccine protection against simian immunodeficiency virus in monkeys using recombinant gamma-2 herpesvirus
    John P Bilello
    New England Primate Research Center, Harvard Medical School, Southborough, MA 01772 9102, USA
    J Virol 85:12708-20. 2011
    ....
  2. pmc Evidence against extracellular exposure of a highly immunogenic region in the C-terminal domain of the simian immunodeficiency virus gp41 transmembrane protein
    Thomas S Postler
    New England Primate Research Center, Department of Microbiology and Immunobiology, Harvard Medical School, Southborough, Massachusetts, USA
    J Virol 86:1145-57. 2012
    ..Together, these data support the conventional model of SIV envelope as a type Ia transmembrane protein with a single membrane-spanning domain and without any extracellular loops...
  3. ncbi request reprint Prospects for an AIDS vaccine
    Ronald C Desrosiers
    New England Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    Nat Med 10:221-3. 2004
  4. pmc Impact of Nef-mediated downregulation of major histocompatibility complex class I on immune response to simian immunodeficiency virus
    Tomek Swigut
    New England Primate Research Center, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 78:13335-44. 2004
    ..These results indicate that nef does serve to limit the virus-specific CD8 cellular response of the host and that the ability to downregulate MHC class I contributes importantly to the totality of nef function...
  5. ncbi request reprint Emergence of cytotoxic T lymphocyte escape mutations in nonpathogenic simian immunodeficiency virus infection
    A Kaur
    Division of Immunology, New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772, USA
    Eur J Immunol 31:3207-17. 2001
    ..These results document the occurrence of CTL escape in a host that does not develop AIDS, and adds to the growing body of evidence that CTL exert significant selective pressure in SIV infection...
  6. pmc Identification of highly attenuated mutants of simian immunodeficiency virus
    R C Desrosiers
    New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    J Virol 72:1431-7. 1998
    ....
  7. pmc Induction of vigorous cytotoxic T-lymphocyte responses by live attenuated simian immunodeficiency virus
    R P Johnson
    Division of Immunology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA
    J Virol 71:7711-8. 1997
    ....
  8. ncbi request reprint Protective immunity induced by live attenuated simian immunodeficiency virus
    R P Johnson
    Division of Immunology, New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772, USA
    Curr Opin Immunol 10:436-43. 1998
    ....
  9. pmc Glycosylation of gp41 of simian immunodeficiency virus shields epitopes that can be targets for neutralizing antibodies
    Eloisa Yuste
    Department of Microbiology and Molecular Genetics, Harvard Medical School, New England Primate Research Center, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 82:12472-86. 2008
    ....
  10. ncbi request reprint Construction and in vitro properties of SIVmac mutants with deletions in "nonessential" genes
    J S Gibbs
    Harvard Medical School, New England Regional Primate Research Center, Southborough, Massachusetts 01772 9102
    AIDS Res Hum Retroviruses 10:607-16. 1994
    ....
  11. ncbi request reprint Effects of reverse-transcriptase mutations M184V and E89G on simian immunodeficiency virus in Rhesus monkeys
    M C Newstein
    Department of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts, USA
    J Infect Dis 184:1262-7. 2001
    ..These results demonstrate that the E89G mutation has a significant negative impact on SIVmac fitness, whereas SIVmac bearing M184V achieved high, sustained virus loads, perhaps with a compensatory effect of the P272S mutation...
  12. ncbi request reprint Construction and in vitro properties of SIVmac mutants with deletions in "nonessential" genes
    J S Gibbs
    Harvard Medical School, New England Regional Primate Research Center, Southborough, Massachusetts 01772 9102
    AIDS Res Hum Retroviruses 10:333-42. 1994
    ....
  13. pmc Ability of the V3 loop of simian immunodeficiency virus to serve as a target for antibody-mediated neutralization: correlation of neutralization sensitivity, growth in macrophages, and decreased dependence on CD4
    R E Means
    Department of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772-9102, USA
    J Virol 75:3903-15. 2001
    ..In addition, these studies suggest a correlation between decreased dependence on CD4 and increased sensitivity to antibody-mediated neutralization...
  14. pmc Efficient transcription and replication of simian immunodeficiency virus in the absence of NF-kappaB and Sp1 binding elements
    P O Ilyinskii
    New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    J Virol 70:3118-26. 1996
    ..Therefore, the surprisingly high replicative capacity of NF-kappaB and Sp1 binding site mutants of SIVmac is due to unique features or the enhancer/promoter region...
  15. ncbi request reprint Construction and in vitro properties of HIV-1 mutants with deletions in "nonessential" genes
    J S Gibbs
    Harvard Medical School, New England Regional Primate Research Center, Southborough, Massachusetts 01772 9102
    AIDS Res Hum Retroviruses 10:343-50. 1994
    ..This collection of HIV-1 deletion mutants will be useful for elucidating the functions of these genes and for investigating antiviral immunity in animal models...
  16. ncbi request reprint Immune evasion strategies of the primate lentiviruses
    D T Evans
    Division of Microbiology, New England Regional Primate Research Center, Harvard Medical School, Southborough, MA 01772-9102, USA
    Immunol Rev 183:141-58. 2001
    ..Here we review these mechanisms of immune evasion considering contributions from both human and non-human primate systems...
  17. ncbi request reprint Comparison of simian immunodeficiency virus isolates
    H W Kestler
    New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
    Nature 331:619-22. 1988
    ..4). These results and other observations provide strong evidence that isolates previously referred to as STLV-IIIAGM and HTLV-IV by others are not authentic, but were derived from cell cultures infected with SIVMAC-251...
  18. pmc Rhesus monkey rhadinovirus ORF57 induces gH and gL glycoprotein expression through posttranscriptional accumulation of target mRNAs
    Young C Shin
    New England Primate Research Center, 1 Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 85:7810-7. 2011
    ....
  19. pmc Simian immunodeficiency virus from the sooty mangabey and rhesus macaque is modified with O-linked carbohydrate
    Elizabeth Stansell
    New England Primate Research Center, Harvard Medical School, Southborough, MA 01772 9102, USA
    J Virol 85:582-95. 2011
    ..These data demonstrate the presence of non- and monosialylated core 1 O-linked carbohydrate on the gp120s of SIVmac and SIVsm and the lack of these modifications on HIV-1 and SIVcpz gp120s...
  20. pmc Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation
    S C Murthy
    New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772
    J Virol 63:3307-14. 1989
    ..5 kilobases). Although some analogies can be drawn between STP and LMP (lymphocyte membrane protein) of Epstein-Barr virus, STP is not related in sequence to LMP...
  21. pmc Simian homologues of human herpesvirus 8
    B Damania
    New England Regional Primate Research Center, Harvard Medical School, One Pine Hill Drive, Box 9102, Southborough, MA 01772-9102, USA
    Philos Trans R Soc Lond B Biol Sci 356:535-43. 2001
    ..Rhadinoviruses that infect hominoids and Old World monkeys include Kaposi's sarcoma-associated herpesvirus, also known as HHV-8, and rhesus monkey rhadinovirus...
  22. pmc A genetic system for rhesus monkey rhadinovirus: use of recombinant virus to quantitate antibody-mediated neutralization
    John P Bilello
    New England Primate Research Center Harvard Medical School, One Pine Hill Drive, P O Box 9102, Southborough, MA 01772 9102, USA
    J Virol 80:1549-62. 2006
    ..This cosmid-based genetic system and the reporter virus neutralization assay will facilitate study of the contribution of individual RRV glycoproteins to entry into different cell types, particularly fibroblasts and B cells...
  23. ncbi request reprint HIV vaccine design: insights from live attenuated SIV vaccines
    Wayne C Koff
    International AIDS Vaccine Initiative, New York, New York 10038, USA
    Nat Immunol 7:19-23. 2006
    ..Here, the strategies defining key components of the protective immune response elicited by these vaccines are discussed...
  24. ncbi request reprint p6gag of human and simian immunodeficiency viruses is tolerant to small in-frame deletions downstream of the late domain
    Cheryl A Pikora
    New England Primate Research Center, Harvard Medical School, One Pine Hill Drive, Southborough, MA 01772, USA
    Virology 346:479-89. 2006
    ..These results demonstrate an overall high tolerance of SIV and HIV to two amino acid deletions within p6gag downstream of the late domain...
  25. pmc Identification of two N-linked glycosylation sites within the core of the simian immunodeficiency virus glycoprotein whose removal enhances sensitivity to soluble CD4
    Cheryl Pikora
    New England Primate Research Center, Harvard Medical School, One Pine Hill Drive, Southborough, MA 01722, USA
    J Virol 79:12575-83. 2005
    ..Thus, glycans at positions 3 and 11 of SIV239 gp120 may be particularly important for shielding the CD4-binding site from antibody recognition...
  26. pmc Virion envelope content, infectivity, and neutralization sensitivity of simian immunodeficiency virus
    Eloisa Yuste
    New England Primate Research Center, Department of Microbiology and Molecular Genetics, Harvard Medical School, Southborough, MA 01772 9102, USA
    J Virol 79:12455-63. 2005
    ..The striking increase in infectivity that results from truncation in the context of SIV316 appears to be due principally to an increase in inherent infectivity per spike...
  27. pmc Effect of CD8+ lymphocyte depletion on virus containment after simian immunodeficiency virus SIVmac251 challenge of live attenuated SIVmac239delta3-vaccinated rhesus macaques
    Jörn E Schmitz
    Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, RE 113, 330 Brookline Ave, Boston, Massacusetts 02215, USA
    J Virol 79:8131-41. 2005
    ....
  28. pmc Simian immunodeficiency virus engrafted with human immunodeficiency virus type 1 (HIV-1)-specific epitopes: replication, neutralization, and survey of HIV-1-positive plasma
    Eloisa Yuste
    New England Primate Research Center, Department of Microbiology and Molecular Genetics, Harvard Medical Scool, One Pine Hill Drive, Box 9102, Southborough, Massachusetts 01772 9102, USA
    J Virol 80:3030-41. 2006
    ..We further conclude that the structures of gp41 from SIVmac239 and HIV-1 are sufficiently similar such that epitopes engrafted into SIVmac239 can be readily recognized by the cognate anti-HIV-1 monoclonal antibodies...
  29. pmc Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus
    Amitinder Kaur
    New England Primate Research Center, Harvard Medical School, One Pine Hill Drive, P O Box 9102, Southborough, MA 01772 9102, USA
    Virology 357:199-214. 2007
    ..05) and peak neutralizing antibody titers pre-challenge (P value 0.06). These findings support continued study of recombinant herpesviruses as a vaccine approach for AIDS...
  30. pmc Induction of a virus-specific effector-memory CD4+ T cell response by attenuated SIV infection
    Marie Claire Gauduin
    Division of Immunology, New England Primate Research Center, Harvard Medical School, Southborough, MA 01772, and Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital, Boston 02115, USA
    J Exp Med 203:2661-72. 2006
    ..The ability of SIVDeltanef to induce a high frequency virus-specific CD4+ T cell response with direct effector function may play a key role in protective immunity produced by vaccination with attenuated SIV strains...
  31. ncbi request reprint A comparative immunogenicity study in rabbits of disulfide-stabilized, proteolytically cleaved, soluble trimeric human immunodeficiency virus type 1 gp140, trimeric cleavage-defective gp140 and monomeric gp120
    Simon Beddows
    Department of Microbiology and Immunology, Weill Medical College of Cornell University, 1300 York Avenue, Room W 805, New York, NY 10021, USA
    Virology 360:329-40. 2007
    ..When antibodies able to neutralize HIV-1(JR-FL) were detected, antigen depletion studies showed they were not directed at the V3 region but were targeted at other, undefined gp120 and also non-gp120 epitopes...
  32. ncbi request reprint Modulation of HIV and SIV neutralization sensitivity by DC-SIGN and mannose-binding lectin
    Andrea Marzi
    Institute for Clinical and Molecular Virology, University Hospital Erlangen, 91054 Erlangen, Germany
    Virology 368:322-30. 2007
    ..Moreover, we provide evidence that mannose-binding lectin (MBL) can interfere with HIV-1 neutralization by the carbohydrate-specific antibody 2G12...
  33. pmc Infectivity and neutralization of simian immunodeficiency virus with FLAG epitope insertion in gp120 variable loops
    Melissa E Laird
    New England Primate Research Center, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 81:10838-48. 2007
    ....
  34. pmc Vaccine protection by live, attenuated simian immunodeficiency virus in the absence of high-titer antibody responses and high-frequency cellular immune responses measurable in the periphery
    Keith Mansfield
    New England Primate Research Center, Harvard Medical School, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 82:4135-48. 2008
    ....
  35. pmc Potent antibody-mediated neutralization and evolution of antigenic escape variants of simian immunodeficiency virus strain SIVmac239 in vivo
    Shuji Sato
    New England Primate Research Center, Department of Microbiology and Molecular Genetics, Harvard Medical School, Southborough, MA 01772, USA
    J Virol 82:9739-52. 2008
    ....
  36. pmc Importance of the V1/V2 loop region of simian-human immunodeficiency virus envelope glycoprotein gp120 in determining the strain specificity of the neutralizing antibody response
    Melissa E Laird
    New England Primate Research Center, One Pine Hill Drive, Southborough, MA 01772 9102, USA
    J Virol 82:11054-65. 2008
    ..These data demonstrate that the V1/V2 region of HIV-1 gp120 is principally responsible for the strain specificity of the neutralizing antibody response in monkeys infected with these prototypic SHIVs...
  37. pmc Immunization of macaques with single-cycle simian immunodeficiency virus (SIV) stimulates diverse virus-specific immune responses and reduces viral loads after challenge with SIVmac239
    David T Evans
    New England Primate Research Center, Department of Microbiology and Molecular Genetics, Harvard Medical School, One Pine Hill Drive, Southborough, MA 01772 9102
    J Virol 79:7707-20. 2005
    ..Given the extraordinary difficulty in protecting against SIV(mac)239, these results are encouraging and support further evaluation of lentiviruses that are limited to a single cycle of infection as a preclinical AIDS vaccine approach...
  38. pmc Retroviral recombination in vivo: viral replication patterns and genetic structure of simian immunodeficiency virus (SIV) populations in rhesus macaques after simultaneous or sequential intravaginal inoculation with SIVmac239Deltavpx/Deltavpr and SIVmac23
    Eun Young Kim
    Division of Infectious Diseases, The Feinberg School of Medicine at Northwestern University, Chicago, Illinois, USA
    J Virol 79:4886-95. 2005
    ..These findings show that recombination can occur readily in vivo after mucosal SIV exposure and thus contributes to the generation of viral genetic diversity and enhancement of viral fitness...
  39. pmc Direct relationship between suppression of virus-specific immunity and emergence of cytomegalovirus disease in simian AIDS
    Amitinder Kaur
    Division of Immunology, New England Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772, USA
    J Virol 77:5749-58. 2003
    ..The underlying mechanism may be a dysfunction of memory B and CD8(+) T lymphocytes associated with SIV-induced impairment of CMV-specific CD4(+) T-cell help...
  40. pmc Effects of cytotoxic T lymphocytes (CTL) directed against a single simian immunodeficiency virus (SIV) Gag CTL epitope on the course of SIVmac239 infection
    Todd M Allen
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI 53715, USA
    J Virol 76:10507-11. 2002
    ..By themselves, these strong CTL responses failed to control SIVmac239 replication...
  41. pmc Importance of B-cell responses for immunological control of variant strains of simian immunodeficiency virus
    Welkin E Johnson
    New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    J Virol 77:375-81. 2003
    ....
  42. pmc Mucosal priming of simian immunodeficiency virus-specific cytotoxic T-lymphocyte responses in rhesus macaques by the Salmonella type III secretion antigen delivery system
    David T Evans
    New England Regional Primate Research Center, Harvard Medical School, One Pine Hill Drive, Southborough, MA 01772 9102, USA
    J Virol 77:2400-9. 2003
    ..Nevertheless, this study demonstrates the potential of mucosal priming by the Salmonella type III secretion system to direct SIV-specific cellular immune responses to the gastrointestinal mucosa in a primate model...
  43. pmc A nucleotide substitution in the tRNA(Lys) primer binding site dramatically increases replication of recombinant simian immunodeficiency virus containing a human immunodeficiency virus type 1 reverse transcriptase
    Kelly Soderberg
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    J Virol 76:5803-6. 2002
    ..RT/SHIV/TC provides an improved system for study of the impact of drug resistance mutations in HIV-1 RT in a relevant animal model...
  44. pmc Tat-vaccinated macaques do not control simian immunodeficiency virus SIVmac239 replication
    Todd M Allen
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53715, USA
    J Virol 76:4108-12. 2002
    ..Despite the induction of Tat-specific CTL, there was no significant reduction in either peak or viral set point compared to that of controls...
  45. pmc Determinants of increased replicative capacity of serially passaged simian immunodeficiency virus with nef deleted in rhesus monkeys
    Louis Alexander
    Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut, USA
    J Virol 77:6823-35. 2003
    ..These results delineate sequence changes in SIV that can compensate for the loss of the nef gene to partially restore replicative and pathogenic potential in rhesus monkeys...
  46. pmc Assorted mutations in the envelope gene of simian immunodeficiency virus lead to loss of neutralization resistance against antibodies representing a broad spectrum of specificities
    Welkin E Johnson
    New England Regional Primate Research Center, Department of Microbiology and Molecular Genetics, Harvard Medical School, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 77:9993-10003. 2003
    ..This reagent set and information should now be useful for defining the physical, structural, thermodynamic, and kinetic factors that influence relative sensitivity to antibody-mediated neutralization...
  47. pmc CD4 independence of simian immunodeficiency virus Envs is associated with macrophage tropism, neutralization sensitivity, and attenuated pathogenicity
    Bridget A Puffer
    Department of Microbiology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Virol 76:2595-605. 2002
    ..Finally, genetic modification of viral Envs to enhance CD4 independence may also result in improved humoral immune responses...
  48. pmc Removal of N-linked glycosylation sites in the V1 region of simian immunodeficiency virus gp120 results in redirection of B-cell responses to V3
    Kelly Stefano Cole
    Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Virol 78:1525-39. 2004
    ..In addition, our results demonstrate that the absence of N-linked carbohydrates at specific sites can influence the exposure of epitopes quite distant in the linear sequence...
  49. ncbi request reprint Public health. A sound rationale needed for phase III HIV-1 vaccine trials
    Dennis R Burton
    Scripps Research Institute, La Jolla, California, USA
    Science 303:316. 2004
  50. pmc Cell tropism of simian immunodeficiency virus in culture is not predictive of in vivo tropism or pathogenesis
    Juan T Borda
    Tulane National Primate Research Center, Tulane University, Covington, LA 70433, USA
    Am J Pathol 165:2111-22. 2004
    ..These data combined with the absence of macrophage-associated lesions in SIVmac239/316-infected animals indicate that in vitro cell tropism is not predictive of in vivo tropism or disease pathogenesis...
  51. ncbi request reprint Viral persistence: HIV's strategies of immune system evasion
    Welkin E Johnson
    New England Regional Primate Research Center, One Pine Hill Drive, Southborough, Massachusetts 01772 9102, USA
    Annu Rev Med 53:499-518. 2002
    ..The future success of antiviral therapies and vaccination strategies may depend largely on understanding how and to what degree each of these factors (and presumably others) contributes to immune evasion...
  52. pmc A novel approach for producing lentiviruses that are limited to a single cycle of infection
    David T Evans
    New England Primate Research Center, Harvard Medical School, Southborough, Massachusetts, USA
    J Virol 78:11715-25. 2004
    ..Single-cycle SIV produced by this approach will be useful for addressing questions relating to viral dynamics and viral pathogenesis and for evaluation as an experimental AIDS vaccine in rhesus macaques...
  53. pmc A replication-competent, neutralization-sensitive variant of simian immunodeficiency virus lacking 100 amino acids of envelope
    Welkin E Johnson
    Department of Microbiology and Molecular Genetics, New England Regional Primate Research Center, One Pine Hill Drive, Box 9102, Southborough, MA 01772 9102, USA
    J Virol 76:2075-86. 2002
    ....
  54. ncbi request reprint HIV vaccine design and the neutralizing antibody problem
    Dennis R Burton
    Departments of Immunology and Molecular Biology, Scripps Research Institute, La Jolla, California, USA
    Nat Immunol 5:233-6. 2004
  55. ncbi request reprint Reversion of CTL escape-variant immunodeficiency viruses in vivo
    Thomas C Friedrich
    Wisconsin National Primate Research Center, Madison, Wisconsin 53715, USA
    Nat Med 10:275-81. 2004
    ..In the absence of selective pressure upon transmission to new hosts, these original escape mutations can be lost. This suggests that some HIV CTL epitopes will be maintained in human populations...
  56. pmc Immunization of rhesus macaques with a DNA prime/modified vaccinia virus Ankara boost regimen induces broad simian immunodeficiency virus (SIV)-specific T-cell responses and reduces initial viral replication but does not prevent disease progression follow
    Helen Horton
    Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, Wisconsin 53715, USA
    J Virol 76:7187-202. 2002
    ..01). However, despite the induction of virus-specific cellular immune responses and reduced peak viral loads, most animals still suffered from gradual CD4 depletion and progressed to disease...
  57. pmc Extreme dependence of gH and gL expression on ORF57 and association with highly unusual codon usage in rhesus monkey rhadinovirus
    John P Bilello
    New England Primate Research Center, Harvard Medical School, Southborough, MA 01772 9102, USA
    J Virol 82:7231-7. 2008
    ....
  58. pmc Envelope glycoprotein cytoplasmic domains from diverse lentiviruses interact with the prenylated Rab acceptor
    David T Evans
    New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772 9102, USA
    J Virol 76:327-37. 2002
    ..Thus, PRA1 associates with envelope glycoproteins from widely divergent lentiviruses...
  59. pmc Cloning and analysis of microRNAs encoded by the primate gamma-herpesvirus rhesus monkey rhadinovirus
    Alexandra Schäfer
    Department of Molecular Genetics and Microbiology and Center for Virology, Duke University Medical Center, Durham, NC 27710, USA
    Virology 364:21-7. 2007
    ..These data set the stage for the mutational ablation and phenotypic analysis of RRV mutants lacking one or more viral microRNAs...
  60. ncbi request reprint PRA1 co-localizes with envelope but does not influence primate lentivirus production, infectivity or envelope incorporation
    Philippe Blancou
    New England Regional Primate Research Center, Harvard Medical School, One Pine Hill Drive, Southborough, MA 01772 9102, USA
    J Gen Virol 86:1785-90. 2005
    ..However, variation in the levels of PRA1 expression did not influence virion production, infectivity or envelope incorporation under the conditions of these assays...
  61. ncbi request reprint A plea for justice for jailed medical workers
    Sunil K Ahuja
    Science 314:924-5. 2006
  62. pmc Infection and persistence of rhesus monkey rhadinovirus in immortalized B-cell lines
    John P Bilello
    New England Primate Research Center, Harvard Medical School, One Pine Hill Drive, Southborough, MA 01772 9102, USA
    J Virol 80:3644-9. 2006
    ..These results establish a naturalistic cell culture system for the study of infection and persistence by RRV in rhesus monkey B cells...
  63. pmc Modulation of Env content in virions of simian immunodeficiency virus: correlation with cell surface expression and virion infectivity
    Eloisa Yuste
    New England Primate Research Center, Harvard Medical School, Southborough, MA 01772 9102, USA
    J Virol 78:6775-85. 2004
    ....
  64. ncbi request reprint An AIDS vaccine: no time to give up
    Dennis R Burton
    Lancet 364:1938. 2004
  65. ncbi request reprint Small nuclear RNAs encoded by Herpesvirus saimiri upregulate the expression of genes linked to T cell activation in virally transformed T cells
    Heidi L Cook
    Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06536, USA
    Curr Biol 15:974-9. 2005
    ..These changes indicate an unexpected role for the HSURs in regulating a remarkably defined and physiologically relevant set of host targets involved in the activation of virally transformed T cells during latency...
  66. pmc Epstein-Barr virus microRNAs are evolutionarily conserved and differentially expressed
    Xuezhong Cai
    Center for Virology, Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA
    PLoS Pathog 2:e23. 2006
    ..Moreover, the differential regulation of EBV miRNA expression implies distinct roles during infection of different human tissues...

Research Grants29

  1. MOLECULAR BASIS FOR SIV PATHOGENICITY
    Ronald Desrosiers; Fiscal Year: 2007
    ..Finally, a potential role for O-linked carbohydrates in shielding antibody access will be investigated. Results from these studies have the potential to enlighten immunogen design for the elicitation of neutralizing antibodies. ..
  2. Rhesus Monkey Rhadinovirus Glycoproteins, Entry, and Neutralization
    Ronald Desrosiers; Fiscal Year: 2009
    ..The experiments will also facilitate use of HHV-8 as a vaccine or gene therapy vector. ..
  3. MOLECULAR BASIS FOR SIV PATHOGENICITY
    Ronald Desrosiers; Fiscal Year: 2009
    ..Results from these studies have the potential to enlighten immunogen design for the elicitation of neutralizing antibodies. ..
  4. Gamma-2 Herpesviruses as Vaccine Vectors for AIDS
    Ronald Desrosiers; Fiscal Year: 2009
    ..Thus, it is not unreasonable to envision a recombinant KSHV that is missing several genes and capable of expressing HIV antigens for use as a vaccine in people. ..
  5. Rhesus Monkey Rhadinovirus Glycoproteins, Entry, and Neutralization
    Ronald C Desrosiers; Fiscal Year: 2010
    ..The experiments will also facilitate use of HHV-8 as a vaccine or gene therapy vector. ..
  6. LIVE ATTENUATED MULTIPLY DELETED HIV1 VACCINE FOR AIDS
    Ronald Desrosiers; Fiscal Year: 2004
    ..Through these experiments we will begin the understand the mechanisms of protective immunity and what is needed to induce it. ..
  7. Contributions of HIV qp41 TM Protein to Pathogenesis
    Ronald Desrosiers; Fiscal Year: 2005
    ..Better understanding of these parameters will eventually fuel vaccine and drug development efforts. ..
  8. MOLECULAR BASIS FOR SIV PATHOGENICITY
    Ronald Desrosiers; Fiscal Year: 1993
    ..If nef is required for the pathogenic potential of the virus, the nef gene product will become an important target for drug development...
  9. 19th Annual Symposium-Nonhuman Primate Models for AIDS
    Ronald Desrosiers; Fiscal Year: 2001
    ..The conference will also include an opening reception on the day of arrival, an evening poster session with accompanying reception and an evening banquet with a topical speaker. ..
  10. MOLECULAR BASIS FOR SIV PATHOGENICITY
    Ronald C Desrosiers; Fiscal Year: 2010
    ..Results from these studies have the potential to enlighten immunogen design for the elicitation of neutralizing antibodies. ..