Affiliation: Harvard University
- Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindlesA Desai
Marine Biological Laboratory, Woods Hole, Massachusetts, USA
J Cell Biol 141:703-13. 1998..We discuss the possible biological implications of the existence of two distinct anaphase A mechanisms and their differential contributions to poleward chromosome movement in different cell types...
- Tubulin and FtsZ structures: functional and therapeutic implicationsA Desai
Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
Bioessays 20:523-7. 1998..This structural inferiority complex has finally been lifted by two recent papers describing the structures of the alpha beta tubulin dimer and FtsZ, a protein similar to tubulin that is essential for cell division in prokaryotes...
- Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindlesT J Mitchison
Marine Biological Laboratory, Woods Hole, MA 02543, USA
Mol Biol Cell 16:3064-76. 2005..The response to microtubule destabilization suggests that an unidentified tensile element acts in parallel with these conventional factors, generating spindle shortening force...
- The spindle: a dynamic assembly of microtubules and motorsT Wittmann
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
Nat Cell Biol 3:E28-34. 2001..Here we describe the structural and dynamic properties of the spindle, and introduce the current concepts regarding how a bipolar spindle is assembled and how it functions to segregate chromosomes...
- Bipolarization and poleward flux correlate during Xenopus extract spindle assemblyT J Mitchison
Cell Division Group, Marine Biological Laboratory, Woods Hole, MA 02543, USA
Mol Biol Cell 15:5603-15. 2004..Our results highlight the importance of two processes, chromatin-initiated microtubule nucleation, and sliding forces generated between antiparallel microtubules, in self-organization of spindle bipolarity and poleward flux...
- XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assemblyC E Walczak
Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143 0450, USA
Cell 84:37-47. 1996..XKCM1 thus exhibits a novel activity for a kinesin-related protein by promoting microtubule depolymerization during mitotic spindle assembly...
- Functional analysis of kinetochore assembly in Caenorhabditis elegansK Oegema
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
J Cell Biol 153:1209-26. 2001..Furthermore, kinetochore assembly and the recruitment of CeINCENP to chromosomes are independent. These results suggest distinct roles for the kinetochore and the chromosomal passengers in mitotic chromosome segregation...
- Implication of a novel multiprotein Dam1p complex in outer kinetochore functionI M Cheeseman
Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
J Cell Biol 155:1137-45. 2001..These and other analyses implicate the four newly identified subunits and the Dam1p complex as a whole in outer kinetochore function where they are well positioned to facilitate the association of chromosomes with spindle microtubules...
- Cytoplasmic forms of fission yeast casein kinase-1 associate primarily with the particulate fraction of the cellP C Wang
Cold Spring Harbor Laboratory, New York 11724 2220
J Biol Chem 269:12014-23. 1994..These results suggest that of the four members of the casein kinase-1 family recognized in fission yeast, one member, Cki2, may contribute to the regulation of cell morphology...
- Purification and assay of a septin complex from Drosophila embryosK Oegema
Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143, USA
Methods Enzymol 298:279-95. 1998
- Lysozyme refolding with cyclodextrins: structure-activity relationshipA Desai
Department of Chemistry, Central Michigan University, Dow 346, Mt. Pleasant, MI 48859, USA
Biochimie 88:1435-45. 2006..CDs may show properties similar to chaotropic agents, which may help explain their anti-aggregation and protein refolding ability. Besides alpha-CD, a number of other neutral CDs were found to be effective protein folding aids...