BRUCE F DEMPLE

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Regulation of heme oxygenase-1 mRNA deadenylation and turnover in NIH3T3 cells by nitrosative or alkylation stress
    Veronica Leautaud
    Department of Genetics and Complex Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    BMC Mol Biol 8:116. 2007
  2. ncbi Radical ideas: genetic responses to oxidative stress
    B Demple
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Clin Exp Pharmacol Physiol 26:64-8. 1999
  3. ncbi Molecular and biological roles of Ape1 protein in mammalian base excision repair
    Bruce Demple
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    DNA Repair (Amst) 4:1442-9. 2005
  4. ncbi Dynamics and diversions in base excision DNA repair of oxidized abasic lesions
    Bruce Demple
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    Oncogene 21:8926-34. 2002
  5. ncbi Signal transduction by nitric oxide in cellular stress responses
    Bruce Demple
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    Mol Cell Biochem 234:11-8. 2002
  6. ncbi Genetic responses against nitric oxide toxicity
    B Demple
    Department of Cancer Cell Biology and Division of Biological Sciences Graduate Program, Harvard School of Public Health, Boston, MA 02115, USA
    Braz J Med Biol Res 32:1417-27. 1999
  7. ncbi Protection from the dark side of NO: signaling and cellular defenses against nitric oxide toxicity
    Bruce Demple
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    IUBMB Life 56:59-64. 2004
  8. ncbi Escherichia coli SoxR protein: sensor/transducer of oxidative stress and nitric oxide
    Bruce Demple
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Methods Enzymol 348:355-64. 2002
  9. pmc Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria
    Pingfang Liu
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Mol Cell Biol 28:4975-87. 2008
  10. pmc Transcription-defective soxR mutants of Escherichia coli: isolation and in vivo characterization
    Monica Chander
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Bacteriol 185:2441-50. 2003

Research Grants

  1. RADIATION BIOLOGY TRAINING PROGRAM
    Bruce Demple; Fiscal Year: 2007

Collaborators

Detail Information

Publications30

  1. pmc Regulation of heme oxygenase-1 mRNA deadenylation and turnover in NIH3T3 cells by nitrosative or alkylation stress
    Veronica Leautaud
    Department of Genetics and Complex Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    BMC Mol Biol 8:116. 2007
    ..The mechanism of this stabilization remains unknown...
  2. ncbi Radical ideas: genetic responses to oxidative stress
    B Demple
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Clin Exp Pharmacol Physiol 26:64-8. 1999
    ..Adaptive resistance to NO depends strongly on the inducible heam oxygenase activity...
  3. ncbi Molecular and biological roles of Ape1 protein in mammalian base excision repair
    Bruce Demple
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    DNA Repair (Amst) 4:1442-9. 2005
    ..Notably, all these effects are reversed by expression of the unrelated protein Apn1 of S. cerevisiae, which shares only the enzymatic repair function with Ape1 (AP endonuclease)...
  4. ncbi Dynamics and diversions in base excision DNA repair of oxidized abasic lesions
    Bruce Demple
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    Oncogene 21:8926-34. 2002
  5. ncbi Signal transduction by nitric oxide in cellular stress responses
    Bruce Demple
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    Mol Cell Biochem 234:11-8. 2002
    ..NO-induced stabilization of mRNA is mediated by pre-existing proteins and points to the existence of an important new signaling pathway that counteracts the damage and stress exerted by this free radical...
  6. ncbi Genetic responses against nitric oxide toxicity
    B Demple
    Department of Cancer Cell Biology and Division of Biological Sciences Graduate Program, Harvard School of Public Health, Boston, MA 02115, USA
    Braz J Med Biol Res 32:1417-27. 1999
    ..Although nitrosylated SoxR is very stable in purified form, the spectroscopic signals for the nitrosylated iron-sulfur centers disappear rapidly in vivo, indicating an active process to reverse or eliminate them...
  7. ncbi Protection from the dark side of NO: signaling and cellular defenses against nitric oxide toxicity
    Bruce Demple
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    IUBMB Life 56:59-64. 2004
    ..In human fibroblasts, this stabilization increases directly with the degree of NO exposure. Novel regulatory pathways appear to underlie the pathways of inducible NO resistance and NO-mediated mRNA stability...
  8. ncbi Escherichia coli SoxR protein: sensor/transducer of oxidative stress and nitric oxide
    Bruce Demple
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Methods Enzymol 348:355-64. 2002
  9. pmc Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria
    Pingfang Liu
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Mol Cell Biol 28:4975-87. 2008
    ..The mitochondrial LP-BER pathway likely plays important roles in repairing dL lesions and other oxidative lesions and perhaps in normal mtDNA replication...
  10. pmc Transcription-defective soxR mutants of Escherichia coli: isolation and in vivo characterization
    Monica Chander
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Bacteriol 185:2441-50. 2003
    ..This work begins the general identification of the residues in the SoxR polypeptide that are critical for transducing oxidative stress signals into gene activation...
  11. ncbi Functional analysis of SoxR residues affecting transduction of oxidative stress signals into gene expression
    Monica Chander
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 279:41603-10. 2004
    ..These findings indicate that SoxR functions as a redox-responsive molecular switch in which subunit interactions transduce a subtle alteration in oxidation state into a profound change in DNA structure...
  12. pmc ATF4-dependent oxidative induction of the DNA repair enzyme Ape1 counteracts arsenite cytotoxicity and suppresses arsenite-mediated mutagenesis
    Hua Fung
    Department of Genetics and Complex Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    Mol Cell Biol 27:8834-47. 2007
    ..Therefore, the ATF4-mediated up-regulation of Ape1 and other genes plays a key role against arsenite-mediated toxicity and mutagenesis...
  13. ncbi DNA repair in mammalian mitochondria: Much more than we thought?
    Pingfang Liu
    Department of Radiation Oncology, Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Environ Mol Mutagen 51:417-26. 2010
    ..Therefore, multiple DNA repair pathways and controlled degradation of mtDNA function together to maintain the integrity of mitochondrial genome...
  14. ncbi Covalent trapping of human DNA polymerase beta by the oxidative DNA lesion 2-deoxyribonolactone
    Michael S DeMott
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 277:7637-40. 2002
    ..Consequently dL residues may not be readily repaired by "short-patch" base excision repair but instead function as suicide substrates in the formation of protein-DNA cross-links that may require alternative modes of repair...
  15. ncbi A key role for heme oxygenase-1 in nitric oxide resistance in murine motor neurons and glia
    Amy Bishop
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    Biochem Biophys Res Commun 325:3-9. 2004
    ..These results confirm the in vivo role of HO1 as a front-line defense against NO toxicity in neuronal cells...
  16. ncbi Action of human apurinic endonuclease (Ape1) on C1'-oxidized deoxyribose damage in DNA
    Yong Jie Xu
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    DNA Repair (Amst) 2:175-85. 2003
    ..The recent observation that Ape1-cleaved dL sites can covalently trap DNA polymerase beta during the abasic excision process suggests that efficient incision of dL by Ape1 may potentiate further problems in DNA repair...
  17. ncbi Genomic instability and aging-like phenotype in the absence of mammalian SIRT6
    Raul Mostoslavsky
    Howard Hughes Medical Institute, The Children s Hospital, CBR Institute for Biomedical Research, Harvard University Medical School, Boston, MA 02115, USA
    Cell 124:315-29. 2006
    ..We conclude that one function of SIRT6 is to promote normal DNA repair, and that SIRT6 loss leads to abnormalities in mice that overlap with aging-associated degenerative processes...
  18. ncbi Nitric oxide-mediated upregulation of the TGF-beta-inducible early response gene-1 (TIEG1) in human fibroblasts by mRNA stabilization independent of TGF-beta
    Minako Mitsumoto
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    Free Radic Biol Med 34:1607-13. 2003
    ..The TIEG1 mRNA now joins heme oxygenase-1 mRNA in displaying regulation by NO-mediated stabilization. It remains to be determined whether the same control mechanism operates on these and perhaps other messages...
  19. ncbi Long-patch base excision DNA repair of 2-deoxyribonolactone prevents the formation of DNA-protein cross-links with DNA polymerase beta
    Jung Suk Sung
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 280:39095-103. 2005
    ..Our observations reveal a cellular repair process for dL lesions that avoids formation of DPCs that would threaten the integrity of DNA and perhaps cell viability...
  20. pmc Constitutive soxR mutations contribute to multiple-antibiotic resistance in clinical Escherichia coli isolates
    Anastasia Koutsolioutsou
    Department of Genetics and Complex Diseases and Division of Biological Sciences, Harvard School of Public Health, 665 Huntington Ave, Boston, Massachusetts 02115, USA
    Antimicrob Agents Chemother 49:2746-52. 2005
    ..The mechanism of such increased soxS expression remains unclear. The same E. coli clinical isolates harbored polymorphic soxR and soxS DNA sequences, also seen for the first time...
  21. ncbi A vital role for Ape1/Ref1 protein in repairing spontaneous DNA damage in human cells
    Hua Fung
    Department of Genetics and Complex Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
    Mol Cell 17:463-70. 2005
    ..Accumulation of abasic DNA damage from intrinsic sources appears sufficient to trigger cell death when Ape1-mediated repair is deficient...
  22. ncbi Nitric oxide-induced apoptosis in lymphoblastoid and fibroblast cells dependent on the phosphorylation and activation of p53
    Laura M McLaughlin
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115 6021, USA
    Cancer Res 65:6097-104. 2005
    ....
  23. ncbi Grasping the message: regulated mRNA stability in free radical stress responses. Rodríguez-Gabriel MA, Burns G, McDonald WH et al. RNA-binding protein Csx1 mediates global control of gene expression in response to oxidative stress. EMBO J 2003; 22: 6256-6
    Bruce Demple
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    Redox Rep 9:3-5. 2004
  24. ncbi Global adjustment of microbial physiology during free radical stress
    Pablo J Pomposiello
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, MA 02115, USA
    Adv Microb Physiol 46:319-41. 2002
    ....
  25. pmc Intrinsic 5'-deoxyribose-5-phosphate lyase activity in Saccharomyces cerevisiae Trf4 protein with a possible role in base excision DNA repair
    Lionel Gellon
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115, USA
    DNA Repair (Amst) 7:187-98. 2008
    ..Finally, we demonstrate that Trf5 significantly affects MMS sensitivity and thus probably BER efficiency in cells expressing either wild-type Trf4 or a C-terminus-deleted form...
  26. ncbi Modulation of the 3'-->5'-exonuclease activity of human apurinic endonuclease (Ape1) by its 5'-incised Abasic DNA product
    Donny Wong
    Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 278:36242-9. 2003
    ..These results suggest roles for the Ape1 exonuclease during BER after both DNA repair synthesis and excision of the abasic deoxyribose-5-phosphate by polymerase beta...
  27. ncbi Resistance to nitric oxide-induced necrosis in heme oxygenase-1 overexpressing pulmonary epithelial cells associated with decreased lipid peroxidation
    Tiffany A Reiter
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 281:36603-12. 2006
    ....
  28. ncbi The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death
    Dipanjan Chowdhury
    CBR Institute for Biomedical Research, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 23:133-42. 2006
    ..After granzyme A activates NM23-H1 to make single-stranded nicks, TREX1 removes nucleotides from the nicked 3' end to reduce the possibility of repair by rejoining the nicked ends...
  29. ncbi Carbon monoxide mediates protection against nitric oxide toxicity in HeLa cells
    Tiffany A Reiter
    Department of Genetics and Complex Diseases, Harvard School of Public Health, 665 Huntington Avenue 1 512, Boston, MA 02115, USA
    Free Radic Biol Med 39:1075-88. 2005
    ..Last, inhibition of p38 MAPK activation fully blocked the CO-protective effect, indicating the involvement of this signaling pathway(s) in the CO response...
  30. ncbi Modulation of the 5'-deoxyribose-5-phosphate lyase and DNA synthesis activities of mammalian DNA polymerase beta by apurinic/apyrimidinic endonuclease 1
    Donny Wong
    Department of Genetics and Complex Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA
    J Biol Chem 279:25268-75. 2004
    ..Simultaneous monitoring of 5'-dRP excision and DNA synthesis by pol beta demonstrated that the 5'-dRP lyase activity lags behind the polymerase activity despite the coordination of these two steps by Ape1 during BER...

Research Grants2

  1. RADIATION BIOLOGY TRAINING PROGRAM
    Bruce Demple; Fiscal Year: 2007
    ....