George Daley

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc A role for Lin28 in primordial germ-cell development and germ-cell malignancy
    Jason A West
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and the Dana Farber Cancer Institute, MA 02115, USA
    Nature 460:909-13. 2009
  2. pmc Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients
    Suneet Agarwal
    Division of Hematology Oncology, Children s Hospital Boston, Massachusetts 02115, USA
    Nature 464:292-6. 2010
  3. pmc Chromatin-modifying enzymes as modulators of reprogramming
    Tamer T Onder
    Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Manton Center for Orphan Disease Research, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 483:598-602. 2012
  4. pmc Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies
    Hao Zhu
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Nat Genet 42:626-30. 2010
  5. pmc Bone-marrow adipocytes as negative regulators of the haematopoietic microenvironment
    Olaia Naveiras
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Massachusetts 02115, USA
    Nature 460:259-63. 2009
  6. pmc Activation of tyrosine kinases by mutation of the gatekeeper threonine
    Mohammad Azam
    Karp Research Building, 7th Floor, Division of Pediatric Hematology Oncology, Children s Hospital of Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:1109-18. 2008
  7. pmc Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
    Kitai Kim
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Nat Biotechnol 29:1117-9. 2011
  8. pmc Euchromatin islands in large heterochromatin domains are enriched for CTCF binding and differentially DNA-methylated regions
    Bo Wen
    Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    BMC Genomics 13:566. 2012
  9. pmc Pluripotent stem cells in research and treatment of hemoglobinopathies
    Natasha Arora
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA, Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA Division of Pediatric Hematology Oncology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Med 2:a011841. 2012
  10. doi request reprint Cellular alchemy and the golden age of reprogramming
    George Q Daley
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 151:1151-4. 2012

Detail Information

Publications88

  1. pmc A role for Lin28 in primordial germ-cell development and germ-cell malignancy
    Jason A West
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and the Dana Farber Cancer Institute, MA 02115, USA
    Nature 460:909-13. 2009
    ....
  2. pmc Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients
    Suneet Agarwal
    Division of Hematology Oncology, Children s Hospital Boston, Massachusetts 02115, USA
    Nature 464:292-6. 2010
    ..Our results demonstrate that reprogramming restores telomere elongation in DC cells despite genetic lesions affecting telomerase, and show that strategies to increase TERC expression may be therapeutically beneficial in DC patients...
  3. pmc Chromatin-modifying enzymes as modulators of reprogramming
    Tamer T Onder
    Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Manton Center for Orphan Disease Research, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 483:598-602. 2012
    ....
  4. pmc Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies
    Hao Zhu
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts, USA
    Nat Genet 42:626-30. 2010
    ..Here we report a mouse that models the human phenotypes associated with genetic variation in the Lin28-Let-7 pathway...
  5. pmc Bone-marrow adipocytes as negative regulators of the haematopoietic microenvironment
    Olaia Naveiras
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Massachusetts 02115, USA
    Nature 460:259-63. 2009
    ..These data implicate adipocytes as predominantly negative regulators of the bone-marrow microenvironment, and indicate that antagonizing marrow adipogenesis may enhance haematopoietic recovery in clinical bone-marrow transplantation...
  6. pmc Activation of tyrosine kinases by mutation of the gatekeeper threonine
    Mohammad Azam
    Karp Research Building, 7th Floor, Division of Pediatric Hematology Oncology, Children s Hospital of Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:1109-18. 2008
    ..These results demonstrate that mutation of the gatekeeper threonine is a common mechanism of activation for tyrosine kinases and provide structural insights to guide the development of next-generation inhibitors...
  7. pmc Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells
    Kitai Kim
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Nat Biotechnol 29:1117-9. 2011
    ..Extended passage of some iPSC clones in culture did not improve their epigenetic resemblance to embryonic stem cells, implying that some human iPSCs retain a residual 'epigenetic memory' of their tissue of origin...
  8. pmc Euchromatin islands in large heterochromatin domains are enriched for CTCF binding and differentially DNA-methylated regions
    Bo Wen
    Center for Epigenetics and Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA
    BMC Genomics 13:566. 2012
    ..The microstructure of these regions has not previously been explored...
  9. pmc Pluripotent stem cells in research and treatment of hemoglobinopathies
    Natasha Arora
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA, Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA Division of Pediatric Hematology Oncology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Cold Spring Harb Perspect Med 2:a011841. 2012
    ..Here we review the progress, promise, and remaining hurdles in realizing the potential of PSCs for cell therapy...
  10. doi request reprint Cellular alchemy and the golden age of reprogramming
    George Q Daley
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 151:1151-4. 2012
    ..In experiments performed nearly 50 years apart, Gurdon and Yamanaka made feasible the reawakening of pluripotency inherent in all cells and challenged forever our notions of cellular identity...
  11. pmc The promise and perils of stem cell therapeutics
    George Q Daley
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02138, USA
    Cell Stem Cell 10:740-9. 2012
    ..Numerous challenges and technical barriers must be overcome before novel stem cell therapies can achieve meaningful clinical impact...
  12. pmc Dynamic instability of genomic methylation patterns in pluripotent stem cells
    Steen Kt Ooi
    Department of Genetics and Development, Columbia University, New York, USA
    Epigenetics Chromatin 3:17. 2010
    ....
  13. ncbi request reprint Gametes from embryonic stem cells: a cup half empty or half full?
    George Q Daley
    Division of Pediatric Hematology Oncology, Children s Hospital Boston, Massachusetts, USA
    Science 316:409-10. 2007
    ..Formation of primordial germ cells is robust, but terminal gametogenesis remains inefficient and doubts about gamete function persist. Although useful for research, clinical use of ES cell-derived gametes appears a distant prospect...
  14. ncbi request reprint Ethics. The ISSCR guidelines for human embryonic stem cell research
    George Q Daley
    Children s Hospital, Boston, Massachusetts, USA
    Science 315:603-4. 2007
  15. doi request reprint Prospects for stem cell-based therapy
    George Q Daley
    Division of Hematology Oncology, Children s Hospital Boston, Boston, MA 02115, USA
    Cell 132:544-8. 2008
    ..However, a number of technical hurdles must be overcome before therapies based on pluripotent human stem cells can enter the clinic...
  16. pmc microRNAs become macro players in somatic cell reprogramming
    Tamer T Onder
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Division of Hematology, Brigham and Women s Hospital, Boston, MA 02115, USA
    Genome Med 3:40. 2011
    ..This new method raises interesting questions about the mechanisms of reprogramming and is likely to facilitate the generation of induced pluripotent stem cells for potential future clinical use...
  17. pmc Stem cells: roadmap to the clinic
    George Q Daley
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Boston, Massachusetts, USA
    J Clin Invest 120:8-10. 2010
    ..They also highlight the challenges to be faced in translating what is indeed highly promising science into proven therapies that will regenerate and repair diseased tissues...
  18. pmc A robust approach to identifying tissue-specific gene expression regulatory variants using personalized human induced pluripotent stem cells
    Je Hyuk Lee
    Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 5:e1000718. 2009
    ..We show that our approach to mapping cis-regulatory variants reduces in vitro experimental noise and reveals additional tissue-specific variants using skin-derived human iPS cells...
  19. ncbi request reprint Therapeutic potential of embryonic stem cells
    Paul H Lerou
    Children s Hospital, Boston, MA 02115, USA
    Blood Rev 19:321-31. 2005
    ..In addition to discussing the therapeutic potential of hESC, this chapter will cover limitations to using hESC for replacement cell therapy, strategies to overcome these limitations, and alternative methods of deriving hESC...
  20. ncbi request reprint Histocompatible embryonic stem cells by parthenogenesis
    Kitai Kim
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Science 315:482-6. 2007
    ..Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation...
  21. pmc Embryonic stem cell-derived hematopoietic stem cells
    Yuan Wang
    Division of Hematology Oncology, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Division of Hematology, Brigham and Women s Hospital, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 102:19081-6. 2005
    ..These data document the cardinal stem cell features of self-renewal and multilineage differentiation of ESC-derived hematopoietic stem cells...
  22. ncbi request reprint LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells
    Laurence Daheron
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
    Stem Cells 22:770-8. 2004
    ..Feeder-free culture conditions that maintain hESCs in an undifferentiated state do not show activation of STAT3, suggesting that distinct signaling mechanisms govern the self-renewal of hESCs...
  23. ncbi request reprint Human embryonic stem cells flock together
    M William Lensch
    Division of Hematology Oncology, Children s Hospital Boston, Karp Building, 7th Floor 300 Longwood Ave Boston, MA 02115, USA
    Nat Biotechnol 25:748-50. 2007
  24. ncbi request reprint Altered nuclear transfer in stem-cell research - a flawed proposal
    Douglas A Melton
    Harvard Stem Cell Institute, Boston, MA, USA
    N Engl J Med 351:2791-2. 2004
  25. doi request reprint Molecular basis of pluripotency
    Lingyi Chen
    Division of Pediatric Hematology Oncology, Children s Hospital Boston, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Hum Mol Genet 17:R23-7. 2008
    ..In this article, we discuss the roles of transcriptional regulation, epigenetic regulation and miRNAs in the maintenance of pluripotency and the differentiation of ES cells...
  26. pmc Ras-MAPK signaling promotes trophectoderm formation from embryonic stem cells and mouse embryos
    Chi Wei Lu
    Division of Pediatric Hematology and Oncology, Children s Hospital Boston and Dana Faber Cancer Institute, Boston, Massachusetts 02115, USA
    Nat Genet 40:921-6. 2008
    ..These data show that ectopic Ras activation can divert ES cells toward extraembryonic trophoblastic fates and implicate Ras-MAPK signaling in promoting trophectoderm formation from mouse embryos...
  27. pmc Scientific and clinical opportunities for modeling blood disorders with embryonic stem cells
    M William Lensch
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Blood 107:2605-12. 2006
    ..The recent availability of human embryonic stem (hES) cells suggests that such a system is now at hand. This review highlights the potential of hES cells to model human hematologic processes in vitro with an emphasis on disease targets...
  28. ncbi request reprint Differentiation potential of histocompatible parthenogenetic embryonic stem cells
    Claudia Lengerke
    Division of Pediatric Hematology Oncology, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    Ann N Y Acad Sci 1106:209-18. 2007
    ..Here, we explore the differentiation potential of murine pESCs derived in our laboratory...
  29. pmc microRNA expression during trophectoderm specification
    Srinivas R Viswanathan
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA, USA
    PLoS ONE 4:e6143. 2009
    ..We have compared miRNA expression in embryonic stem cell (ESC)-derived trophectoderm and in staged murine embryos to identify a set of candidate miRNAs likely to be involved in trophectoderm specification...
  30. ncbi request reprint Stem cell research: science, ethics and policy
    George Q Daley
    Division of Hematology Oncology, Children s Hospital, Boston, MA, USA
    Med Ethics (Burlingt Mass) 12:5. 2005
  31. pmc Distinct and combinatorial functions of Jmjd2b/Kdm4b and Jmjd2c/Kdm4c in mouse embryonic stem cell identity
    Partha Pratim Das
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Division of Hematology Oncology, Boston Children s Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 53:32-48. 2014
    ..Thus, two HDMs of the same subclass exhibit distinct and combinatorial functions in control of the ESC state. Such complexity of HDM function reveals an aspect of multilayered transcriptional control. ..
  32. doi request reprint Isolation of hematopoietic stem cells from mouse embryonic stem cells
    Shannon L McKinney-Freeman
    Division of Pediatric Hematology Oncology, Children s Hospital, Boston, Massachusetts, USA
    Curr Protoc Stem Cell Biol . 2008
    ....
  33. ncbi request reprint Reprogramming of human somatic cells to pluripotency with defined factors
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    Nature 451:141-6. 2008
    ..These data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture...
  34. pmc Upping the ante: recent advances in direct reprogramming
    Lars U W Müller
    Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Mol Ther 17:947-53. 2009
    ..We also highlight recent advances that eliminate stable genetic modification from the reprogramming process, and summarize preclinical models that provide proof-of-concept for ES/iPS cell-based regenerative medicine...
  35. doi request reprint Human iPS cell derivation/reprogramming
    In Hyun Park
    Children s Hospital Boston and Dana Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts, USA
    Curr Protoc Stem Cell Biol . 2009
    ..Human iPS cells share similarities with hES cells including the expression of pluripotency genes, and differentiation as embryoid bodies in vitro into three germ layers (EB) and in vivo as teratomas...
  36. pmc Selective blockade of microRNA processing by Lin28
    Srinivas R Viswanathan
    Stem Cell Program, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
    Science 320:97-100. 2008
    ..Our results identify Lin28 as a negative regulator of miRNA biogenesis and suggest that Lin28 may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers...
  37. pmc Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells
    Sabine Loewer
    Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Manton Center for Orphan Disease Research, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Nat Genet 42:1113-7. 2010
    ....
  38. doi request reprint Cross-regulation of the Nanog and Cdx2 promoters
    Lingyi Chen
    Division of Pediatric Hematology Oncology, Children s Hospital Boston, Dana Farber Cancer Institute, Boston, MA, USA
    Cell Res 19:1052-61. 2009
    ..However, whereas Nanog knockout results in detectable Cdx2 expression in the ICM, we observe no overt disruption of blastocyst development, indicating that Nanog plays a subservient role to Oct4 in segregation of the ICM and TE...
  39. ncbi request reprint Missed opportunities in embryonic stem-cell research
    George Q Daley
    Children s Hospital and Harvard Medical School, Boston, USA
    N Engl J Med 351:627-8. 2004
  40. doi request reprint Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells
    Elayne M Chan
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts, USA
    Nat Biotechnol 27:1033-7. 2009
    ..Our data define molecular markers of the fully reprogrammed state and highlight the need for rigorous characterization and standardization of putative iPS cells...
  41. ncbi request reprint Male germ cells
    Niels Geijsen
    Harvard Stem Cell Institute, Center for Regenerative Medicine and Technology, Boston, Massachusetts, USA
    Methods Enzymol 418:307-14. 2006
    ..This chapter details methods for in vitro derivation of germ lineage elements and discusses potential applications of these techniques in germ cell research...
  42. ncbi request reprint High-efficiency RNA interference in human embryonic stem cells
    Holm Zaehres
    Harvard Stem Cell Institute and Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology and Division of Hematology Oncology, Children s Hospital, Boston, Massachusetts 02115, USA
    Stem Cells 23:299-305. 2005
    ..Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal...
  43. ncbi request reprint In vitro gametogenesis from embryonic stem cells
    Jason A West
    Graduate Program of Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Opin Cell Biol 16:688-92. 2004
    ....
  44. doi request reprint Lin28: A microRNA regulator with a macro role
    Srinivas R Viswanathan
    Children s Hospital Boston and Dana Farber Cancer Institute, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, and the Howard Hughes Medical Institute, MA 02115, USA
    Cell 140:445-9. 2010
    ..This role for Lin28 has important implications for our mechanistic understanding of pluripotency, the timing of development, and oncogenesis...
  45. ncbi request reprint In vitro generation of germ cells from murine embryonic stem cells
    Jason A West
    Graduate Program of Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA
    Nat Protoc 1:2026-36. 2006
    ..It serves as a platform for studying the poorly understood process of germ cell allocation, imprint erasure and gamete formation, with 4-6 weeks being required to isolate PGCs as well as haploid cells...
  46. pmc Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis
    Trista E North
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 447:1007-11. 2007
    ..The conserved role for PGE2 in the regulation of vertebrate HSC homeostasis indicates that modulation of the prostaglandin pathway may facilitate expansion of HSC number for therapeutic purposes...
  47. ncbi request reprint Teratoma formation assays with human embryonic stem cells: a rationale for one type of human-animal chimera
    M William Lensch
    Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell Stem Cell 1:253-8. 2007
    ..We argue that this experiment raises no significant moral concerns and should be the jurisdiction of animal care and use committees and exempt from formal review by the stem cell research oversight process...
  48. pmc Genomic approaches to deconstruct pluripotency
    Yuin Han Loh
    Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Children s Hospital Boston, Massachusetts 02115, USA
    Annu Rev Genomics Hum Genet 12:165-85. 2011
    ..In this review, we will discuss recent advances gleaned from application of global "omics" techniques to dissect the molecular mechanisms that define the pluripotent state...
  49. pmc Generation of induced pluripotent stem cells from human blood
    Yuin Han Loh
    Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Blood 113:5476-9. 2009
    ..The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage...
  50. pmc Biomechanical forces promote embryonic haematopoiesis
    Luigi Adamo
    Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 459:1131-5. 2009
    ..Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development...
  51. pmc Lin28 promotes transformation and is associated with advanced human malignancies
    Srinivas R Viswanathan
    Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA, USA
    Nat Genet 41:843-8. 2009
    ..Our work provides a mechanism for the coordinate repression of let-7 miRNAs observed in a subset of human cancers, and associates activation of LIN28 and LIN28B with poor clinical prognosis...
  52. pmc All-trans retinoic acid directs urothelial specification of murine embryonic stem cells via GATA4/6 signaling mechanisms
    Joshua R Mauney
    Urological Diseases Research Center, Children s Hospital Boston, Boston, Massachusetts, USA
    PLoS ONE 5:e11513. 2010
    ..Collectively, these data suggest that RA mediates ESC specification toward a urothelial lineage via GATA4/6-dependent processes...
  53. pmc Interaction of retinoic acid and scl controls primitive blood development
    Jill L O de Jong
    Stem Cell Program and Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, USA
    Blood 116:201-9. 2010
    ..Our studies establish a new connection between RA and scl during development that may participate in stem cell self-renewal and hematopoietic differentiation...
  54. pmc Disease-specific induced pluripotent stem cells
    In Hyun Park
    Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 134:877-86. 2008
    ..Such disease-specific stem cells offer an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development...
  55. doi request reprint From fibroblasts to iPS cells: induced pluripotency by defined factors
    Rui Zhao
    Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
    J Cell Biochem 105:949-55. 2008
    ..In addition, though not yet proven experimentally, overcoming cellular senescence of fibroblasts by inactivating Rb and p53 pathways and up-regulating telomerase activity may also be required...
  56. doi request reprint Human embryonic stem cell derivation from poor-quality embryos
    Paul H Lerou
    Division of Newborn Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    Nat Biotechnol 26:212-4. 2008
    ..Early-arrested or highly fragmented embryos only rarely yield cell lines, whereas those that have achieved blastocyst stage are a robust source of normal hES cells...
  57. doi request reprint Enhanced plating efficiency of trypsin-adapted human embryonic stem cells is reversible and independent of trisomy 12/17
    Elayne M Chan
    Division of Hematology Oncology, Children s Hospital, Boston, Massachusetts 02115, USA
    Cloning Stem Cells 10:107-18. 2008
    ..Nevertheless, the high plating efficiency of trypsin passaged hESCs is a reversible phenotype, regardless of chromosomal abnormalities, suggesting that epigenetic events are responsible for the switch in phenotype...
  58. pmc Modulation of murine embryonic stem cell-derived CD41+c-kit+ hematopoietic progenitors by ectopic expression of Cdx genes
    Shannon L McKinney-Freeman
    Division of Pediatric Hematology Oncology, Children s Hospital, Boston, MA
    Blood 111:4944-53. 2008
    ..The behavior of Cdx genes in vitro suggests how derangement of these developmental regulators might contribute to leukemogenesis...
  59. doi request reprint BMP and Wnt specify hematopoietic fate by activation of the Cdx-Hox pathway
    Claudia Lengerke
    Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell Stem Cell 2:72-82. 2008
    ..Our data suggest that BMP signaling plays two distinct and sequential roles during blood formation, initially as an inducer of mesoderm, and later to specify blood via activation of Wnt signaling and the Cdx-Hox pathway...
  60. doi request reprint Determinants of microRNA processing inhibition by the developmentally regulated RNA-binding protein Lin28
    Elena Piskounova
    Stem Cell Program, Children s Hospital Boston, the Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    J Biol Chem 283:21310-4. 2008
    ..These findings establish a regulatory role for the terminal loop of precursors in miRNA maturation and provide insight into the mechanism by which Lin28 negatively regulates let-7 processing...
  61. doi request reprint Generation of human-induced pluripotent stem cells
    In Hyun Park
    Division of Pediatric Hematology Oncology, Children s Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Protoc 3:1180-6. 2008
    ..Overall, it takes 2 months to complete reprogramming human primary fibroblasts starting from biopsy...
  62. pmc Hemogenic endothelial progenitor cells isolated from human umbilical cord blood
    Xiao Wu
    Vascular Biology Program and Department of Surgery, Children s Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Stem Cells 25:2770-6. 2007
    ..Three of 17 clonal cell populations gave rise to early CFU-GEMM hematopoietic progenitors and burst-forming unit-erythroid progenitors. These results provide evidence for hemogenic endothelial cells in human umbilical cord blood...
  63. ncbi request reprint Nuclear transplantation, embryonic stem cells and the potential for cell therapy
    Konrad Hochedlinger
    Whitehead Institute, Cambridge, MA, USA
    Hematol J 5:S114-7. 2004
    ..Moreover, we will discuss the potential use of nuclear transfer to study the role of reversible genomic (epigenetic) modifications during tumorigenesis...
  64. ncbi request reprint Derivation of embryonic germ cells and male gametes from embryonic stem cells
    Niels Geijsen
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
    Nature 427:148-54. 2004
    ..Our ability to derive germ cells from embryonic stem cells provides an accessible in vitro model system for studies of germline epigenetic modification and mammalian gametogenesis...
  65. ncbi request reprint Cloning and stem cells--handicapping the political and scientific debates
    George Q Daley
    Whitehead Institute for Biomedical Research, Cambridge, USA
    N Engl J Med 349:211-2. 2003
  66. ncbi request reprint ES cells prove egg-straordinary
    George Q Daley
    Nat Biotechnol 21:760-1. 2003
  67. ncbi request reprint Prospects for stem cell therapeutics: myths and medicines
    George Q Daley
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge Massachusetts 02142, USA
    Curr Opin Genet Dev 12:607-13. 2002
    ..These cells have yielded modest and preliminary hints of functional reconstitution in animal models. Although encouraging, significant hurdles remain before the promise of stem cells will be realized in the clinic...
  68. ncbi request reprint Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy
    William M Rideout
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 109:17-27. 2002
    ..Mature myeloid and lymphoid cells as well as immunoglobulins became detectable 3-4 weeks after transplantation. Our results establish a paradigm for the treatment of a genetic disorder by combining therapeutic cloning with gene therapy...
  69. ncbi request reprint Patterning definitive hematopoietic stem cells from embryonic stem cells
    Claudia Lengerke
    Department of Hematology and Oncology, University Medical Center II, Tuebingen, Germany
    Exp Hematol 33:971-9. 2005
    ....
  70. ncbi request reprint Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systems
    Stephen M Dang
    Institute of Biomaterials and Biomedical Engineering, 4 Taddle Creek Road, Rm 407, Rosebrugh Building, Toronto ON, Canada, M5S 3G9
    Biotechnol Bioeng 78:442-53. 2002
    ..These results provide a foundation for development of efficient, scalable bioprocesses for ES cell differentiation, and inform novel methods for the production of hematopoietic tissues...
  71. ncbi request reprint HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors
    Michael Kyba
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Cell 109:29-37. 2002
    ..This strategy for blood engraftment enables modeling of hematopoietic transplantation from ES cells...
  72. ncbi request reprint Current prospects for the generation of patient-specific pluripotent cells from adult tissues
    Suneet Agarwal
    Regen Med 2:743-52. 2007
  73. ncbi request reprint A role for thrombopoietin in hemangioblast development
    Rita C R Perlingeiro
    Whitehead Institute, Cambridge, Massachusetts 02142, USA
    Stem Cells 21:272-80. 2003
    ..These results establish that Mpl signaling plays a role in the earliest stages of hematopoietic development and that TPO represents a third growth factor influencing hemangioblast formation...
  74. ncbi request reprint From embryos to embryoid bodies: generating blood from embryonic stem cells
    George Q Daley
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Ann N Y Acad Sci 996:122-31. 2003
    ..Comparable approaches with human ES cells are being developed to lay the foundation for cellular therapies in patients with a variety of bone marrow diseases...
  75. ncbi request reprint Debugging cellular reprogramming
    In Hyun Park
    Nat Cell Biol 9:871-3. 2007
  76. pmc Acceleration of mesoderm development and expansion of hematopoietic progenitors in differentiating ES cells by the mouse Mix-like homeodomain transcription factor
    Stephen Willey
    Department of Medicine, Mt Sinai School of Medicine, New York, NY 10029 6574, USA
    Blood 107:3122-30. 2006
    ..Therefore, the mouse Mix gene functions early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages...
  77. pmc Enhanced hematopoietic differentiation of embryonic stem cells conditionally expressing Stat5
    Michael Kyba
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 100:11904-10. 2003
    ....
  78. ncbi request reprint Hematopoiesis from embryonic stem cells: lessons from and for ontogeny
    Michael Kyba
    Center for Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Tex, USA
    Exp Hematol 31:994-1006. 2003
    ..In this review, we evaluate the work that has been done to date on the hematopoietic differentiation of ES cells, and discuss this in the context of what is known about the embryonic origin of the hematopoietic stem cell...
  79. ncbi request reprint Development of hematopoietic repopulating cells from embryonic stem cells
    Michael Kyba
    Center for Developmental Biology, UT Southwestern Medical Center, Dallas, Texas 75390 9133, USA
    Methods Enzymol 365:114-29. 2003
  80. pmc Multivariate proteomic analysis of murine embryonic stem cell self-renewal versus differentiation signaling
    Wendy Prudhomme
    Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:2900-5. 2004
    ..Our results demonstrate how a computational systems biology approach can elucidate key sets of intracellular signaling protein activities that combine to govern cell phenotypic responses to extracellular cues...
  81. ncbi request reprint Taking stock and planning for the next decade: realistic prospects for stem cell therapies for the nervous system
    Evan Y Snyder
    The Burnham Institute, La Jolla, California 92037, USA
    J Neurosci Res 76:157-68. 2004
    ..Despite the admonitions to be circumspect, we also suggest disease processes that may be within the grasp of proven stem cell properties and might be approachable in the relatively near future...
  82. ncbi request reprint Origins of mammalian hematopoiesis: in vivo paradigms and in vitro models
    M William Lensch
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
    Curr Top Dev Biol 60:127-96. 2004
    ....
  83. ncbi request reprint Customized human embryonic stem cells
    George Q Daley
    Nat Biotechnol 23:826-8. 2005
  84. pmc The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiation
    Atsushi Kubo
    Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
    Blood 105:4590-7. 2005
    ....
  85. ncbi request reprint Inducible transgene expression in mouse stem cells
    David T Ting
    Harvard M I T Program in Health Sciences and Technology, Harvard Medical School Boston, MA, USA
    Methods Mol Med 105:23-46. 2005
    ..This demonstration is one of many possible uses for this powerful and versatile system...
  86. ncbi request reprint Bayesian analysis of signaling networks governing embryonic stem cell fate decisions
    Peter J Woolf
    Department of Chemical Engineering, University of Michigan, Room 3320, G G Brown Building, 2300 Hayward Street, Ann Arbor, MI 48109 2125, USA
    Bioinformatics 21:741-53. 2005
    ..Our experimental dataset includes measurements for 28 signaling protein phosphorylation states across 16 different factorial combinations of cytokine and matrix stimuli as reported previously...

Research Grants27

  1. Hematopoietic stem cells from totipotent stem cell types
    George Daley; Fiscal Year: 2004
    ..Future efforts will explore the similarities and differences in the hematopoietic potential of murine and human ES cells, making use of repopulation studies of human cells in NOD/Scid mice. ..
  2. Therapeutic Mechanisms in Chronic Myelogenous Leukemia
    George Daley; Fiscal Year: 2001
    ..This proposal seeks to advance our fundamental knowledge of CML biology and therapeutic mechanisms in hopes of refining future treatment strategies. ..
  3. Hematopoietic Stem Cells from Pluripotent Embryonic Stem Cells
    George Daley; Fiscal Year: 2009
    ..These experiments address classic questions in developmental hematopoiesis, and establish important principles for future attempts that we will make to derive HSCs from human ESCs (WA09/H9 and UC06/HSF6). ..
  4. Hematopoietic Stem Cells from Pluripotent Embryonic Stem Cells
    George Daley; Fiscal Year: 2007
    ..These experiments address classic questions in developmental hematopoiesis, and establish important principles for future attempts that we will make to derive HSCs from human ESCs (WA09/H9 and UC06/HSF6). ..
  5. Therapeutic Mechanisms in Chronic Myelogenous Leukemia
    George Daley; Fiscal Year: 2004
    ..This proposal seeks to advance our fundamental knowledge of CML biology and therapeutic mechanisms in hopes of refining future treatment strategies. ..
  6. Modeling Developmental Hematopoiesis with Embryonic Stem
    George Daley; Fiscal Year: 2004
    ..Our goal is to carefully define hematopoietic development from human ES cells, and to establish a pre-clinical platform for the hES- based cell therapies of the future. ..
  7. Hematopoietic stem cells from totipotent stem cell types
    George Daley; Fiscal Year: 2003
    ..Future efforts will explore the similarities and differences in the hematopoietic potential of murine and human ES cells, making use of repopulation studies of human cells in NOD/Scid mice. ..
  8. Therapeutic Mechanisms in Chronic Myelogenous Leukemia
    George Daley; Fiscal Year: 2003
    ..This proposal seeks to advance our fundamental knowledge of CML biology and therapeutic mechanisms in hopes of refining future treatment strategies. ..
  9. Modeling Developmental Hematopoiesis with Embryonic Stem
    George Daley; Fiscal Year: 2003
    ..Our goal is to carefully define hematopoietic development from human ES cells, and to establish a pre-clinical platform for the hES- based cell therapies of the future. ..
  10. Hematopoietic stem cells from totipotent stem cell types
    George Daley; Fiscal Year: 2001
    ..Future efforts will explore the similarities and differences in the hematopoietic potential of murine and human ES cells, making use of repopulation studies of human cells in NOD/Scid mice. ..
  11. Murine Models for Regenerative Medicine
    George Daley; Fiscal Year: 2009
    ..These studies will provide an important test of the feasibility of therapeutic applications of embryonic stem cells. ..
  12. MOLECULAR PATHWAYS IN MYELOPROLIFERATIVE DISEASE
    George Daley; Fiscal Year: 2002
    ....
  13. Modeling Developmental Hematopoiesis with Embryonic Stem
    George Daley; Fiscal Year: 2002
    ..Our goal is to carefully define hematopoietic development from human ES cells, and to establish a pre-clinical platform for the hES- based cell therapies of the future. ..
  14. Murine Models for Regenerative Medicine
    George Daley; Fiscal Year: 2007
    ..These studies will provide an important test of the feasibility of therapeutic applications of embryonic stem cells. ..
  15. Hematopoietic Stem Cells from Pluripotent Embryonic Stem Cells
    George Daley; Fiscal Year: 2006
    ..These experiments address classic questions in developmental hematopoiesis, and establish important principles for future attempts that we will make to derive HSCs from human ESCs (WA09/H9 and UC06/HSF6). ..
  16. Murine Models for Regenerative Medicine
    George Daley; Fiscal Year: 2006
    ..These studies will provide an important test of the feasibility of therapeutic applications of embryonic stem cells. ..
  17. Hematopoietic Stem Cells from Pluripotent Embryonic Stem Cells
    George Daley; Fiscal Year: 2005
    ..These experiments address classic questions in developmental hematopoiesis, and establish important principles for future attempts that we will make to derive HSCs from human ESCs (WA09/H9 and UC06/HSF6). ..
  18. Therapeutic Mechanisms in Chronic Myelogenous Leukemia
    George Daley; Fiscal Year: 2005
    ..This proposal seeks to advance our fundamental knowledge of CML biology and therapeutic mechanisms in hopes of refining future treatment strategies. ..
  19. Modeling Developmental Hematopoiesis with Embryonic Stem
    George Daley; Fiscal Year: 2005
    ..Our goal is to carefully define hematopoietic development from human ES cells, and to establish a pre-clinical platform for the hES- based cell therapies of the future. ..
  20. Therapeutic Mechanisms in Chronic Myelogenous Leukemia
    George Daley; Fiscal Year: 2003
    ..This proposal seeks to advance our fundamental knowledge of CML biology and therapeutic mechanisms in hopes of refining future treatment strategies. ..
  21. Therapeutic Mechanisms in Chronic Myelogenous Leukemia
    George Daley; Fiscal Year: 2002
    ..This proposal seeks to advance our fundamental knowledge of CML biology and therapeutic mechanisms in hopes of refining future treatment strategies. ..
  22. Modeling Developmental Hematopoiesis with Embryonic Stem
    George Daley; Fiscal Year: 2003
    ..Our goal is to carefully define hematopoietic development from human ES cells, and to establish a pre-clinical platform for the hES- based cell therapies of the future. ..
  23. Hematopoietic stem cells from totipotent stem cell types
    George Daley; Fiscal Year: 2002
    ..Future efforts will explore the similarities and differences in the hematopoietic potential of murine and human ES cells, making use of repopulation studies of human cells in NOD/Scid mice. ..
  24. Hematopoietic stem cells from totipotent stem cell types
    George Daley; Fiscal Year: 2003
    ..Future efforts will explore the similarities and differences in the hematopoietic potential of murine and human ES cells, making use of repopulation studies of human cells in NOD/Scid mice. ..
  25. Murine Models for Regenerative Medicine
    George Daley; Fiscal Year: 2009
    ..These studies will provide an important test of the feasibility of therapeutic applications of embryonic stem cells. ..