Research Topics
| George DaleySummaryAffiliation: Harvard University Country: USA Publications
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Publications
Pluripotent stem cells in research and treatment of hemoglobinopathiesNatasha Arora
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA, Harvard Stem Cell Institute, Cambridge, Massachusetts 02138, USA Division of Pediatric Hematology Oncology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
Cold Spring Harb Perspect Med 2:a011841. 2012..Here we review the progress, promise, and remaining hurdles in realizing the potential of PSCs for cell therapy...
Cellular alchemy and the golden age of reprogrammingGeorge Q Daley
Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
Cell 151:1151-4. 2012..In experiments performed nearly 50 years apart, Gurdon and Yamanaka made feasible the reawakening of pluripotency inherent in all cells and challenged forever our notions of cellular identity...- The promise and perils of stem cell therapeuticsGeorge Q Daley
Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02138, USA
Cell Stem Cell 10:740-9. 2012..Numerous challenges and technical barriers must be overcome before novel stem cell therapies can achieve meaningful clinical impact... - Dynamic instability of genomic methylation patterns in pluripotent stem cellsSteen Kt Ooi
Department of Genetics and Development, Columbia University, New York, USA
Epigenetics Chromatin 3:17. 2010.... - microRNAs become macro players in somatic cell reprogrammingTamer T Onder
Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Children s Hospital Boston and Dana Farber Cancer Institute, Division of Hematology, Brigham and Women s Hospital, Boston, MA 02115, USA
Genome Med 3:40. 2011..This new method raises interesting questions about the mechanisms of reprogramming and is likely to facilitate the generation of induced pluripotent stem cells for potential future clinical use... - Prospects for stem cell-based therapyGeorge Q Daley
Division of Hematology Oncology, Children s Hospital Boston, Boston, MA 02115, USA
Cell 132:544-8. 2008..However, a number of technical hurdles must be overcome before therapies based on pluripotent human stem cells can enter the clinic... 
Gametes from embryonic stem cells: a cup half empty or half full?George Q Daley
Division of Pediatric Hematology Oncology, Children s Hospital Boston, Massachusetts, USA
Science 316:409-10. 2007..Formation of primordial germ cells is robust, but terminal gametogenesis remains inefficient and doubts about gamete function persist. Although useful for research, clinical use of ES cell-derived gametes appears a distant prospect...- Ethics. The ISSCR guidelines for human embryonic stem cell researchGeorge Q Daley
Children's Hospital, Boston, Massachusetts, USA
Science 315:603-4. 2007 - Stem cells: roadmap to the clinicGeorge Q Daley
Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Boston, Massachusetts, USA
J Clin Invest 120:8-10. 2010..They also highlight the challenges to be faced in translating what is indeed highly promising science into proven therapies that will regenerate and repair diseased tissues... - A robust approach to identifying tissue-specific gene expression regulatory variants using personalized human induced pluripotent stem cellsJe Hyuk Lee
Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Genet 5:e1000718. 2009..We show that our approach to mapping cis-regulatory variants reduces in vitro experimental noise and reveals additional tissue-specific variants using skin-derived human iPS cells... - Therapeutic potential of embryonic stem cellsPaul H Lerou
Children s Hospital, Boston, MA 02115, USA
Blood Rev 19:321-31. 2005..In addition to discussing the therapeutic potential of hESC, this chapter will cover limitations to using hESC for replacement cell therapy, strategies to overcome these limitations, and alternative methods of deriving hESC... - Histocompatible embryonic stem cells by parthenogenesisKitai Kim
Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
Science 315:482-6. 2007..Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation... - LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cellsLaurence Daheron
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
Stem Cells 22:770-8. 2004..Feeder-free culture conditions that maintain hESCs in an undifferentiated state do not show activation of STAT3, suggesting that distinct signaling mechanisms govern the self-renewal of hESCs... - Embryonic stem cell-derived hematopoietic stem cellsYuan Wang
Division of Hematology/Oncology, Children's Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Division of Hematology, Brigham and Women's Hospital, Harvard Stem Cell Institute, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 102:19081-6. 2005..These data document the cardinal stem cell features of self-renewal and multilineage differentiation of ESC-derived hematopoietic stem cells... - Altered nuclear transfer in stem-cell research - a flawed proposalDouglas A Melton
Harvard Stem Cell Institute, Boston, MA, USA
N Engl J Med 351:2791-2. 2004 - Differentiation potential of histocompatible parthenogenetic embryonic stem cellsClaudia Lengerke
Division of Pediatric Hematology Oncology, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
Ann N Y Acad Sci 1106:209-18. 2007..Here, we explore the differentiation potential of murine pESCs derived in our laboratory... - Stem cell research: science, ethics and policyGeorge Q Daley
Division of Hematology/Oncology, Children's Hospital, Boston, MA, USA
Med Ethics 12:5. 2005 - Scientific and clinical opportunities for modeling blood disorders with embryonic stem cellsM William Lensch
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
Blood 107:2605-12. 2006..The recent availability of human embryonic stem (hES) cells suggests that such a system is now at hand. This review highlights the potential of hES cells to model human hematologic processes in vitro with an emphasis on disease targets... - Ras-MAPK signaling promotes trophectoderm formation from embryonic stem cells and mouse embryosChi Wei Lu
Division of Pediatric Hematology and Oncology, Children s Hospital Boston and Dana Faber Cancer Institute, Boston, Massachusetts 02115, USA
Nat Genet 40:921-6. 2008..These data show that ectopic Ras activation can divert ES cells toward extraembryonic trophoblastic fates and implicate Ras-MAPK signaling in promoting trophectoderm formation from mouse embryos... - microRNA expression during trophectoderm specificationSrinivas R Viswanathan
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA, USA
PLoS ONE 4:e6143. 2009..We have compared miRNA expression in embryonic stem cell (ESC)-derived trophectoderm and in staged murine embryos to identify a set of candidate miRNAs likely to be involved in trophectoderm specification... - Human embryonic stem cells flock togetherM William Lensch
Division of Hematology/Oncology, Children's Hospital Boston, Karp Building, 7th Floor 300 Longwood Ave. Boston, MA 02115, USA
Nat Biotechnol 25:748-50. 2007 - Molecular basis of pluripotencyLingyi Chen
Division of Pediatric Hematology Oncology, Children s Hospital Boston, Dana Farber Cancer Institute, Boston, MA 02115, USA
Hum Mol Genet 17:R23-7. 2008..In this article, we discuss the roles of transcriptional regulation, epigenetic regulation and miRNAs in the maintenance of pluripotency and the differentiation of ES cells... - Selective blockade of microRNA processing by Lin28Srinivas R Viswanathan
Stem Cell Program, Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02115, USA
Science 320:97-100. 2008..Our results identify Lin28 as a negative regulator of miRNA biogenesis and suggest that Lin28 may play a central role in blocking miRNA-mediated differentiation in stem cells and in certain cancers... - Teratoma formation assays with human embryonic stem cells: a rationale for one type of human-animal chimeraM William Lensch
Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
Cell Stem Cell 1:253-8. 2007..We argue that this experiment raises no significant moral concerns and should be the jurisdiction of animal care and use committees and exempt from formal review by the stem cell research oversight process... - Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasisTrista E North
Stem Cell Program and Division of Hematology Oncology, Children s Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 447:1007-11. 2007..The conserved role for PGE2 in the regulation of vertebrate HSC homeostasis indicates that modulation of the prostaglandin pathway may facilitate expansion of HSC number for therapeutic purposes... - Reprogramming of human somatic cells to pluripotency with defined factorsIn Hyun Park
Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
Nature 451:141-6. 2008..These data demonstrate that defined factors can reprogramme human cells to pluripotency, and establish a method whereby patient-specific cells might be established in culture... - Isolation of hematopoietic stem cells from mouse embryonic stem cellsShannon L McKinney-Freeman
Division of Pediatric Hematology Oncology, Children s Hospital, Boston, Massachusetts, USA
Curr Protoc Stem Cell Biol . 2008.... - Human iPS cell derivation/reprogrammingIn Hyun Park
Children s Hospital Boston and Dana Farber Cancer Institute, Harvard Medical School, Harvard Stem Cell Institute, Boston, Massachusetts, USA
Curr Protoc Stem Cell Biol . 2009..Human iPS cells share similarities with hES cells including the expression of pluripotency genes, and differentiation as embryoid bodies in vitro into three germ layers (EB) and in vivo as teratomas... - In vitro generation of germ cells from murine embryonic stem cellsJason A West
Graduate Program of Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts, USA
Nat Protoc 1:2026-36. 2006..It serves as a platform for studying the poorly understood process of germ cell allocation, imprint erasure and gamete formation, with 4-6 weeks being required to isolate PGCs as well as haploid cells... - Missed opportunities in embryonic stem-cell researchGeorge Q Daley
Children's Hospital and Harvard Medical School, Boston, USA
N Engl J Med 351:627-8. 2004 - Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cellsElayne M Chan
Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts, USA
Nat Biotechnol 27:1033-7. 2009..Our data define molecular markers of the fully reprogrammed state and highlight the need for rigorous characterization and standardization of putative iPS cells... - A role for Lin28 in primordial germ-cell development and germ-cell malignancyJason A West
Division of Pediatric Hematology Oncology, Children s Hospital Boston and the Dana Farber Cancer Institute, MA 02115, USA
Nature 460:909-13. 2009.... - Cross-regulation of the Nanog and Cdx2 promotersLingyi Chen
Division of Pediatric Hematology Oncology, Children s Hospital Boston, Dana Farber Cancer Institute, Boston, MA, USA
Cell Res 19:1052-61. 2009..However, whereas Nanog knockout results in detectable Cdx2 expression in the ICM, we observe no overt disruption of blastocyst development, indicating that Nanog plays a subservient role to Oct4 in segregation of the ICM and TE... - Male germ cellsNiels Geijsen
Harvard Stem Cell Institute, Center for Regenerative Medicine and Technology, Boston, Massachusetts, USA
Methods Enzymol 418:307-14. 2006..This chapter details methods for in vitro derivation of germ lineage elements and discusses potential applications of these techniques in germ cell research... - In vitro gametogenesis from embryonic stem cellsJason A West
Graduate Program of Biological and Biomedical Sciences, Harvard Medical School, Boston, Massachusetts 02115, USA
Curr Opin Cell Biol 16:688-92. 2004.... - High-efficiency RNA interference in human embryonic stem cellsHolm Zaehres
Harvard Stem Cell Institute and Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology and Division of Hematology Oncology, Children s Hospital, Boston, Massachusetts 02115, USA
Stem Cells 23:299-305. 2005..Gene knockdown of Oct4 and Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal... - Lin28: A microRNA regulator with a macro roleSrinivas R Viswanathan
Children s Hospital Boston and Dana Farber Cancer Institute, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, and the Howard Hughes Medical Institute, MA 02115, USA
Cell 140:445-9. 2010..This role for Lin28 has important implications for our mechanistic understanding of pluripotency, the timing of development, and oncogenesis... - Upping the ante: recent advances in direct reprogrammingLars U W Müller
Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
Mol Ther 17:947-53. 2009..We also highlight recent advances that eliminate stable genetic modification from the reprogramming process, and summarize preclinical models that provide proof-of-concept for ES/iPS cell-based regenerative medicine... - Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cellsSabine Loewer
Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Manton Center for Orphan Disease Research, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts, USA
Nat Genet 42:1113-7. 2010.... - Genomic approaches to deconstruct pluripotencyYuin Han Loh
Stem Cell Transplantation Program, Division of Pediatric Hematology Oncology, Children s Hospital Boston, Massachusetts 02115, USA
Annu Rev Genomics Hum Genet 12:165-85. 2011..In this review, we will discuss recent advances gleaned from application of global "omics" techniques to dissect the molecular mechanisms that define the pluripotent state... - Generation of human-induced pluripotent stem cellsIn Hyun Park
Division of Pediatric Hematology Oncology, Children s Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
Nat Protoc 3:1180-6. 2008..Overall, it takes 2 months to complete reprogramming human primary fibroblasts starting from biopsy... - Generation of induced pluripotent stem cells from human bloodYuin Han Loh
Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, Dana Farber Cancer Institute, Boston, MA 02115, USA
Blood 113:5476-9. 2009..The ability to reprogram cells from human blood will allow the generation of patient-specific stem cells for diseases in which the disease-causing somatic mutations are restricted to cells of the hematopoietic lineage... - Determinants of microRNA processing inhibition by the developmentally regulated RNA-binding protein Lin28Elena Piskounova
Stem Cell Program, Children s Hospital Boston, the Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
J Biol Chem 283:21310-4. 2008..These findings establish a regulatory role for the terminal loop of precursors in miRNA maturation and provide insight into the mechanism by which Lin28 negatively regulates let-7 processing... - All-trans retinoic acid directs urothelial specification of murine embryonic stem cells via GATA4/6 signaling mechanismsJoshua R Mauney
Urological Diseases Research Center, Children s Hospital Boston, Boston, Massachusetts, USA
PLoS ONE 5:e11513. 2010..Collectively, these data suggest that RA mediates ESC specification toward a urothelial lineage via GATA4/6-dependent processes... - Interaction of retinoic acid and scl controls primitive blood developmentJill L O de Jong
Stem Cell Program and Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Harvard Medical School, Boston, MA, USA
Blood 116:201-9. 2010..Our studies establish a new connection between RA and scl during development that may participate in stem cell self-renewal and hematopoietic differentiation... - Disease-specific induced pluripotent stem cellsIn Hyun Park
Department of Medicine, Division of Pediatric Hematology Oncology, Children s Hospital Boston, and Dana Farber Cancer Institute, Boston, MA 02115, USA
Cell 134:877-86. 2008..Such disease-specific stem cells offer an unprecedented opportunity to recapitulate both normal and pathologic human tissue formation in vitro, thereby enabling disease investigation and drug development... - Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patientsSuneet Agarwal
Division of Hematology Oncology, Children s Hospital Boston, Massachusetts 02115, USA
Nature 464:292-6. 2010..Our results demonstrate that reprogramming restores telomere elongation in DC cells despite genetic lesions affecting telomerase, and show that strategies to increase TERC expression may be therapeutically beneficial in DC patients... - Biomechanical forces promote embryonic haematopoiesisLuigi Adamo
Center for Excellence in Vascular Biology, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
Nature 459:1131-5. 2009..Collectively, these data reveal a critical role for biomechanical forces in haematopoietic development... - Lin28 promotes transformation and is associated with advanced human malignanciesSrinivas R Viswanathan
Children s Hospital Boston, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA, USA
Nat Genet 41:843-8. 2009..Our work provides a mechanism for the coordinate repression of let-7 miRNAs observed in a subset of human cancers, and associates activation of LIN28 and LIN28B with poor clinical prognosis... - From fibroblasts to iPS cells: induced pluripotency by defined factorsRui Zhao
Division of Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, Massachusetts 02215, USA
J Cell Biochem 105:949-55. 2008..In addition, though not yet proven experimentally, overcoming cellular senescence of fibroblasts by inactivating Rb and p53 pathways and up-regulating telomerase activity may also be required... - BMP and Wnt specify hematopoietic fate by activation of the Cdx-Hox pathwayClaudia Lengerke
Division of Pediatric Hematology Oncology, Children s Hospital Boston and Dana Farber Cancer Institute, Boston, MA 02115, USA
Cell Stem Cell 2:72-82. 2008..Our data suggest that BMP signaling plays two distinct and sequential roles during blood formation, initially as an inducer of mesoderm, and later to specify blood via activation of Wnt signaling and the Cdx-Hox pathway... - Modulation of murine embryonic stem cell-derived CD41+c-kit+ hematopoietic progenitors by ectopic expression of Cdx genesShannon L McKinney-Freeman
Division of Pediatric Hematology Oncology, Children s Hospital, Boston, MA
Blood 111:4944-53. 2008..The behavior of Cdx genes in vitro suggests how derangement of these developmental regulators might contribute to leukemogenesis... - Enhanced plating efficiency of trypsin-adapted human embryonic stem cells is reversible and independent of trisomy 12/17Elayne M Chan
Division of Hematology Oncology, Children s Hospital, Boston, Massachusetts 02115, USA
Cloning Stem Cells 10:107-18. 2008..Nevertheless, the high plating efficiency of trypsin passaged hESCs is a reversible phenotype, regardless of chromosomal abnormalities, suggesting that epigenetic events are responsible for the switch in phenotype... - Human embryonic stem cell derivation from poor-quality embryosPaul H Lerou
Division of Newborn Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
Nat Biotechnol 26:212-4. 2008..Early-arrested or highly fragmented embryos only rarely yield cell lines, whereas those that have achieved blastocyst stage are a robust source of normal hES cells... - Hemogenic endothelial progenitor cells isolated from human umbilical cord bloodXiao Wu
Vascular Biology Program and Department of Surgery, Children s Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
Stem Cells 25:2770-6. 2007..Three of 17 clonal cell populations gave rise to early CFU-GEMM hematopoietic progenitors and burst-forming unit-erythroid progenitors. These results provide evidence for hemogenic endothelial cells in human umbilical cord blood... - Nuclear transplantation, embryonic stem cells and the potential for cell therapyKonrad Hochedlinger
Whitehead Institute, Cambridge, MA, USA
Hematol J 5:S114-7. 2004..Moreover, we will discuss the potential use of nuclear transfer to study the role of reversible genomic (epigenetic) modifications during tumorigenesis... - Cloning and stem cells--handicapping the political and scientific debatesGeorge Q Daley
Whitehead Institute for Biomedical Research, Cambridge, USA
N Engl J Med 349:211-2. 2003 - ES cells prove egg-straordinaryGeorge Q Daley
Nat Biotechnol 21:760-1. 2003 - Patterning definitive hematopoietic stem cells from embryonic stem cellsClaudia Lengerke
Department of Hematology and Oncology, University Medical Center II, Tuebingen, Germany
Exp Hematol 33:971-9. 2005.... - Prospects for stem cell therapeutics: myths and medicinesGeorge Q Daley
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge Massachusetts 02142, USA
Curr Opin Genet Dev 12:607-13. 2002..These cells have yielded modest and preliminary hints of functional reconstitution in animal models. Although encouraging, significant hurdles remain before the promise of stem cells will be realized in the clinic... - Correction of a genetic defect by nuclear transplantation and combined cell and gene therapyWilliam M Rideout
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell 109:17-27. 2002..Mature myeloid and lymphoid cells as well as immunoglobulins became detectable 3-4 weeks after transplantation. Our results establish a paradigm for the treatment of a genetic disorder by combining therapeutic cloning with gene therapy... - Derivation of embryonic germ cells and male gametes from embryonic stem cellsNiels Geijsen
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Nature 427:148-54. 2004..Our ability to derive germ cells from embryonic stem cells provides an accessible in vitro model system for studies of germline epigenetic modification and mammalian gametogenesis... - A role for thrombopoietin in hemangioblast developmentRita C R Perlingeiro
Whitehead Institute, Cambridge, Massachusetts 02142, USA
Stem Cells 21:272-80. 2003..These results establish that Mpl signaling plays a role in the earliest stages of hematopoietic development and that TPO represents a third growth factor influencing hemangioblast formation... - From embryos to embryoid bodies: generating blood from embryonic stem cellsGeorge Q Daley
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Ann N Y Acad Sci 996:122-31. 2003..Comparable approaches with human ES cells are being developed to lay the foundation for cellular therapies in patients with a variety of bone marrow diseases... - HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitorsMichael Kyba
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell 109:29-37. 2002..This strategy for blood engraftment enables modeling of hematopoietic transplantation from ES cells... - Enhanced hematopoietic differentiation of embryonic stem cells conditionally expressing Stat5Michael Kyba
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
Proc Natl Acad Sci U S A 100:11904-10. 2003.... - Hematopoiesis from embryonic stem cells: lessons from and for ontogenyMichael Kyba
Center for Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Tex, USA
Exp Hematol 31:994-1006. 2003..In this review, we evaluate the work that has been done to date on the hematopoietic differentiation of ES cells, and discuss this in the context of what is known about the embryonic origin of the hematopoietic stem cell... - Development of hematopoietic repopulating cells from embryonic stem cellsMichael Kyba
Center for Developmental Biology, UT Southwestern Medical Center, Dallas, Texas 75390-9133, USA
Methods Enzymol 365:114-29. 2003 - Multivariate proteomic analysis of murine embryonic stem cell self-renewal versus differentiation signalingWendy Prudhomme
Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 101:2900-5. 2004..Our results demonstrate how a computational systems biology approach can elucidate key sets of intracellular signaling protein activities that combine to govern cell phenotypic responses to extracellular cues... - Acceleration of mesoderm development and expansion of hematopoietic progenitors in differentiating ES cells by the mouse Mix-like homeodomain transcription factorStephen Willey
Department of Medicine, Mt Sinai School of Medicine, New York, NY 10029 6574, USA
Blood 107:3122-30. 2006..Therefore, the mouse Mix gene functions early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages... - Current prospects for the generation of patient-specific pluripotent cells from adult tissuesSuneet Agarwal
Regen Med 2:743-52. 2007 - Debugging cellular reprogrammingIn-Hyun Park
Nat Cell Biol 9:871-3. 2007 - Customized human embryonic stem cellsGeorge Q Daley
Nat Biotechnol 23:826-8. 2005 - The homeobox gene HEX regulates proliferation and differentiation of hemangioblasts and endothelial cells during ES cell differentiationAtsushi Kubo
Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA
Blood 105:4590-7. 2005.... - Inducible transgene expression in mouse stem cellsDavid T Ting
Harvard-M.I.T. Program in Health Sciences and Technology, Harvard Medical School. Boston, MA, USA
Methods Mol Med 105:23-46. 2005..This demonstration is one of many possible uses for this powerful and versatile system... - Bayesian analysis of signaling networks governing embryonic stem cell fate decisionsPeter J Woolf
Department of Chemical Engineering, University of Michigan, Room 3320, G G Brown Building, 2300 Hayward Street, Ann Arbor, MI 48109 2125, USA
Bioinformatics 21:741-53. 2005..We demonstrate that the Bayesian networks can capture the linear, nonlinear and multistate logic interactions that connect extracellular cues, intracellular signals and consequent cell functional responses... - Origins of mammalian hematopoiesis: in vivo paradigms and in vitro modelsM William Lensch
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Curr Top Dev Biol 60:127-96. 2004.... - Taking stock and planning for the next decade: realistic prospects for stem cell therapies for the nervous systemEvan Y Snyder
The Burnham Institute, La Jolla, California 92037, USA
J Neurosci Res 76:157-68. 2004..Despite the admonitions to be circumspect, we also suggest disease processes that may be within the grasp of proven stem cell properties and might be approachable in the relatively near future... - Efficiency of embryoid body formation and hematopoietic development from embryonic stem cells in different culture systemsStephen M Dang
Institute of Biomaterials and Biomedical Engineering, 4 Taddle Creek Road, Rm 407, Rosebrugh Building, Toronto ON, Canada, M5S 3G9
Biotechnol Bioeng 78:442-53. 2002..These results provide a foundation for development of efficient, scalable bioprocesses for ES cell differentiation, and inform novel methods for the production of hematopoietic tissues...
Research Grants
- Modeling Developmental Hematopoiesis with Embryonic StemGeorge Daley; Fiscal Year: 2005..Our goal is to carefully define hematopoietic development from human ES cells, and to establish a pre-clinical platform for the hES- based cell therapies of the future. ..
- Therapeutic Mechanisms in Chronic Myelogenous LeukemiaGeorge Daley; Fiscal Year: 2005..This proposal seeks to advance our fundamental knowledge of CML biology and therapeutic mechanisms in hopes of refining future treatment strategies. ..
- Hematopoietic Stem Cells from Pluripotent Embryonic Stem CellsGeorge Daley; Fiscal Year: 2009..These experiments address classic questions in developmental hematopoiesis, and establish important principles for future attempts that we will make to derive HSCs from human ESCs (WA09/H9 and UC06/HSF6). ..
- MOLECULAR PATHWAYS IN MYELOPROLIFERATIVE DISEASEGeorge Daley; Fiscal Year: 2002....
- Hematopoietic stem cells from totipotent stem cell typesGeorge Daley; Fiscal Year: 2004..Future efforts will explore the similarities and differences in the hematopoietic potential of murine and human ES cells, making use of repopulation studies of human cells in NOD/Scid mice. ..
- Murine Models for Regenerative MedicineGeorge Daley; Fiscal Year: 2009..These studies will provide an important test of the feasibility of therapeutic applications of embryonic stem cells. ..