Research Topics
Genomes and Genes
| David E ClaphamSummaryAffiliation: Harvard University Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Sorting out MIC, TRP, and CRAC ion channelsDavid E Clapham
Howard Hughes Medical Institute Children's Hospital, Harvard Medical School, Boston, MA 02115, USA
J Gen Physiol 120:217-20. 2002- Citral sensing by Transient [corrected] receptor potential channels in dorsal root ganglion neuronsStephanie C Stotz
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Boston, Massachusetts, United States of America
PLoS ONE 3:e2082. 2008..Citral's broad spectrum and prolonged sensory inhibition may prove more useful than capsaicin for allodynia, itch, or other types of pain involving superficial sensory nerves and skin... 
Symmetry, selectivity, and the 2003 Nobel PrizeDavid E Clapham
Howard Hughes Medical Institute, Children's Hospital of Boston, Boston, MA 02115, USA
Cell 115:641-6. 2003- Calcium signalingDavid E Clapham
Aldo R Castañeda Professor of Cardiovascular Research, Professor of Neurobiology, Harvard Medical School, Howard Hughes Medical Institute, Enders 1309, Children s Hospital Boston, Boston, MA 02115, USA
Cell 131:1047-58. 2007..Also discussed is the highly localized nature of Ca(2+)-mediated signal transduction and its specific roles in excitability, exocytosis, motility, apoptosis, and transcription... - International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channelsDavid E Clapham
Howard Hughes Medical Institute, Children's Hospital, Boston, MA 02115, USA
Pharmacol Rev 57:427-50. 2005 - International Union of Pharmacology. L. Nomenclature and structure-function relationships of CatSper and two-pore channelsDavid E Clapham
Howard Hughes Medical Institute, Children's Hospital, Boston, MA 02115, USA
Pharmacol Rev 57:451-4. 2005 - TRP channels as cellular sensorsDavid E Clapham
Howard Hughes Medical Institute, Pediatric Cardiology, Children s Hospital of Boston, Department of Neurobiology, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 426:517-24. 2003..They are an ancient sensory apparatus for the cell, not just the multicellular organism, and they have been adapted to respond to all manner of stimuli, from both within and outside the cell... - Signal transduction. Hot and cold TRP ion channelsDavid E Clapham
Howard Hughes Medical Institute, Cardiovascular Division, Children's Hospital, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
Science 295:2228-9. 2002 - A thermodynamic framework for understanding temperature sensing by transient receptor potential (TRP) channelsDavid E Clapham
Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, and Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 108:19492-7. 2011..This postulate, along with the laws of thermodynamics and independent of mechanistic detail, leads to the conclusion that hot- and cold-sensing TRPs operate by identical conformational changes... - Rapid vesicular translocation and insertion of TRP channelsVassilios J Bezzerides
Howard Hughes Medical Institute, Cardiovascular Department, Children's Hospital and Harvard Medical School, Enders 1309 320 Longwood Avenue, Boston, MA 02115, USA
Nat Cell Biol 6:709-20. 2004..The increase in TRPC5 availability affects neurite extension rates in cultured hippocampal neurons, and may be a general mechanism for initiating Ca(2+) influx and cell morphological changes in response to stimuli... - Formation of novel TRPC channels by complex subunit interactions in embryonic brainCarsten Strübing
Howard Hughes Medical Institute, Department of Cardiovascular Research, Children s Hospital and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 278:39014-9. 2003..This complex assembly mechanism increases the diversity of TRPC channels in mammalian brain and may generate novel heteromers that have specific roles in the developing brain... 
TRPM1 forms ion channels associated with melanin content in melanocytesElena Oancea
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
Sci Signal 2:ra21. 2009..We propose that TRPM1 is an ion channel whose function is critical to normal melanocyte pigmentation and is thus a potential target for pigmentation disorders...- Intracellular calcium strongly potentiates agonist-activated TRPC5 channelsNathaniel T Blair
Howard Hughes Medical Institute, Department of Cardiology and Manton Center for Orphan Disease, Children s Hospital Boston, and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
J Gen Physiol 133:525-46. 2009..Ca(2+)-dependent potentiation should result in TRPC5 channel activation preferentially during periods of repetitive firing or coincident neurotransmitter receptor activation... - All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motilityHuayu Qi
Department of Cardiology, Howard Hughes Medical Institute, and Department of Neurobiology, Harvard Medical School, Enders 1309, Children s Hospital Boston, 320 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:1219-23. 2007..Direct protein interactions among CatSpers, the sperm specificity of these proteins, and loss of ICatSper in each of the four CatSper-/- mice indicate that CatSpers are highly specialized flagellar proteins... - The mitochondrial calcium uniporter is a highly selective ion channelYuriy Kirichok
Howard Hughes Medical Institute, Department of Cardiovascular Research, Children's Hospital, andHarvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 427:360-4. 2004..The channel is inwardly rectifying, making it especially effective for Ca2+ uptake into energized mitochondria. Thus, we conclude that the properties of the current mediated by this novel channel are those of the MCU... - The TRPM7 ion channel functions in cholinergic synaptic vesicles and affects transmitter releaseGrigory Krapivinsky
Howard Hughes Medical Institute, Cardiology, Children s Hospital Boston, 1309 Enders Building, 320 Longwood Avenue, Boston, MA, USA
Neuron 52:485-96. 2006..Targeted peptide interference of TRPM7's interaction with snapin affects the amplitudes and kinetics of postsynaptic EPSPs. Thus, vesicular TRPM7 channel activity is critical to neurotransmitter release in sympathetic neurons... - Cleavage of TRPM7 releases the kinase domain from the ion channel and regulates its participation in Fas-induced apoptosisBimal N Desai
Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, and Department of Neurobiology, Harvard Medical School, Enders Building 1309, 320 Longwood Avenue, Boston, MA 02115, USA
Dev Cell 22:1149-62. 2012..These findings raise the possibility that other members of the TRP channel superfamily are also regulated by caspase-mediated cleavage, with wide-ranging implications for cell death and differentiation... - POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transportersGrigory Krapivinsky
Department of Cardiology, The Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 108:19234-9. 2011..Store depletion promotes STIM1-POST complex binding to smooth ER and plasma membrane Ca(2+) ATPases (SERCAs and PMCAs, respectively), Na/K-ATPase, as well as to the nuclear transporters, importins-β and exportins... - SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiationGrigory Krapivinsky
Howard Hughes Medical Institute, Children s Hospital, 1309 Enders Building, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
Neuron 43:563-74. 2004..siRNA-mediated SynGAP knockdown confirmed these results. These data implicate SynGAP in NMDAR- and CaMKII-dependent regulation of AMPAR trafficking... - Phosphatidylinositol 3-kinase activates ERK in primary sensory neurons and mediates inflammatory heat hyperalgesia through TRPV1 sensitizationZhi Ye Zhuang
Pain Research Center, Department of Anesthesiology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
J Neurosci 24:8300-9. 2004..Therefore, PI3K induces heat hyperalgesia, possibly by regulating TRPV1 activity, in an ERK-dependent manner. The PI3K pathway also appears to play a role that is distinct from ERK by regulating the early onset of inflammatory pain... - TRPM7 facilitates cholinergic vesicle fusion with the plasma membraneSebastian Brauchi
Department of Cardiology, Howard Hughes Medical Institute, Children s Hospital Boston, MA 02115, USA
Proc Natl Acad Sci U S A 105:8304-8. 2008..We conclude that the conductance of TRPM7 across the vesicle membrane is important in SSLV fusion... - The channel kinase, TRPM7, is required for early embryonic developmentJie Jin
Department of Cardiology, Manton Center for Orphan Disease, Children s Hospital Boston, Howard Hughes Medical Institute, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 109:E225-33. 2012..The in vivo and in vitro results demonstrate a temporal requirement for the Trpm7 channel kinase during embryogenesis... - Camphor activates and strongly desensitizes the transient receptor potential vanilloid subtype 1 channel in a vanilloid-independent mechanismHaoxing Xu
Howard Hughes Medical Institute, Children's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Neurosci 25:8924-37. 2005..We also found that camphor inhibited several other related TRP channels, including ankyrin-repeat TRP 1 (TRPA1). The camphor-induced desensitization of TRPV1 and block of TRPA1 may underlie the analgesic effects of camphor... - A voltage-gated proton-selective channel lacking the pore domainI Scott Ramsey
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 440:1213-6. 2006..The data presented here identify H(v)1 as a long-sought voltage-gated H+ channel and establish H(v)1 as the founding member of a family of mammalian VSD proteins... - A superfamily of voltage-gated sodium channels in bacteriaRyuta Koishi
Howard Hughes Medical Institute, Department of Cardiovascular Research, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 279:9532-8. 2004..Thus, the Na(V)Bac superfamily is fertile ground for crystallographic, electrophysiological, and microbiological studies... - KSper, a pH-sensitive K+ current that controls sperm membrane potentialBetsy Navarro
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:7688-92. 2007..Alkalinization activates the pH(i)-sensitive I(KSper), setting the membrane potential to negative potentials where Ca2+ entry via I(CatSper) is maximized. I(KSper) is one of two dominant ion currents of capacitated sperm cells... - Oregano, thyme and clove-derived flavors and skin sensitizers activate specific TRP channelsHaoxing Xu
Howard Hughes Medical Institute, Harvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
Nat Neurosci 9:628-35. 2006..Our results support a role for temperature-sensitive TRP channels in chemesthesis in oral and nasal epithelium and suggest that TRPV3 may be a molecular target of plant-derived skin sensitizers... - Ion channels that control fertility in mammalian spermatozoaBetsy Navarro
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Department of Neurobiology, Harvard Medical School, Enders 1309, Children s Hospital Boston, 320 Longwood Avenue, Boston, MA 02115, USA
Int J Dev Biol 52:607-13. 2008..Intracellular alkalinization activates both channels and induces hyperactivated motility... - Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channelYuriy Kirichok
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital and Harvard Medical School Enders 1309, Boston, Massachusetts 02115, USA
Nature 439:737-40. 2006..Our results identify CatSper1 as a component of the key flagellar calcium channel, and suggest that intracellular alkalinization potentiates CatSper current to increase intraflagellar calcium and induce sperm hyperactivation... - TRPC5 is a regulator of hippocampal neurite length and growth cone morphologyAnna Greka
Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
Nat Neurosci 6:837-45. 2003..Dominant-negative TRPC5 expression allowed significantly longer neurites and filopodia to form. We conclude that TRPC5 channels are important components of the mechanism controlling neurite extension and growth cone morphology... - ATP-activated P2X2 current in mouse spermatozoaBetsy Navarro
Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 108:14342-7. 2011..However, fertility of P2rx2(-/-) males declines with frequent mating over days, suggesting that P2X2 receptor adds a selection advantage under these conditions... - Transient receptor potential cation channel, subfamily C, member 5 (TRPC5) is a cold-transducer in the peripheral nervous systemKatharina Zimmermann
Department of Cardiology, Manton Center for Orphan Disease, and Harvard Medical School, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 108:18114-9. 2011..These findings demonstrate that highly cold-sensitive TRPC5 channels are a molecular component for detection and regional adaptation to cold temperatures in the peripheral nervous system that is distinct from noxious cold sensing... - Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporterDawei Jiang
Department of Cardiology, Howard Hughes Medical Institute, Children s Hospital Boston, Manton Center for Orphan Disease, and Department of Neurobiology, Harvard Medical School, Enders Building 1309, 320 Longwood Avenue, Boston, MA 02115, USA
Science 326:144-7. 2009..RNAi knockdown, overexpression, and liposome reconstitution of the purified Letm1 protein demonstrate that Letm1 is a mitochondrial Ca2+/H+ antiporter... - Deletion of Trpm7 disrupts embryonic development and thymopoiesis without altering Mg2+ homeostasisJie Jin
Department of Cardiology, Howard Hughes Medical Institute, Children s Hospital Boston, Boston, MA 02115, USA
Science 322:756-60. 2008..The thymic medullary cells lost signal transducer and activator of transcription 3 activity, which accounts for their depletion when Trpm7 is disrupted in thymocytes... - Controlled delivery of bioactive molecules into live cells using the bacterial mechanosensitive channel MscLJulia F Doerner
HHMI, Department of Cardiology, Boston Children s Hospital, Boston, Massachusetts 02115, USA
Nat Commun 3:990. 2012..We propose that MscL will be a useful tool for gated and controlled delivery of bioactive molecules into cells... - A spontaneous, recurrent mutation in divalent metal transporter-1 exposes a calcium entry pathwayHaoxing Xu
Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Boston, Massachusetts, USA
PLoS Biol 2:E50. 2004..Here we show that G185R mutant DMT1 exhibits a new, constitutive Ca(2+) permeability, suggesting a gain of function that contributes to remutation and the mk and b phenotypes... - An aqueous H+ permeation pathway in the voltage-gated proton channel Hv1I Scott Ramsey
Howard Hughes Medical Institute, Department of Cardiology and Manton Center for Orphan Disease, Children s Hospital Boston, Boston, Massachusetts, USA
Nat Struct Mol Biol 17:869-75. 2010..Our results indicate that Hv1 most likely forms an internal water wire for selective proton transfer and that interactions between water molecules and S4 arginines may underlie coupling between voltage- and pH-gradient sensing... - Calpain cleaves and activates the TRPC5 channel to participate in semaphorin 3A-induced neuronal growth cone collapseJ Stefan Kaczmarek
Program in Neuroscience, Division of Medical Sciences, Harvard University, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 109:7888-92. 2012..Thus, TRPC5 acts downstream of semaphorin signaling to cause changes in neuronal growth cone morphology and nervous system development... - TRPV3 is a calcium-permeable temperature-sensitive cation channelHaoxing Xu
Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
Nature 418:181-6. 2002..On the basis of these properties, we propose that hTRPV3 is thermosensitive in the physiological range of temperatures between TRPM8 and TRPV1... - International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channelsDavid E Clapham
Howard Hughes Medical Institute, Children s Hospital, 1309 Enders Building, 320 Longwood Ave, Boston, MA 02115, USA
Pharmacol Rev 55:591-6. 2003..The complete Compendium, including data tables for each member of the TRP channel family, can be found at http://www.iuphar-db.org/iuphar-ic/... - The NMDA receptor is coupled to the ERK pathway by a direct interaction between NR2B and RasGRF1Grigory Krapivinsky
Howard Hughes Medical Institute, Children s Hospital, 1309 Enders Building, 320 Longwood Avenue, Boston, MA 02115, USA
Neuron 40:775-84. 2003..The specific association of RasGRF1 with the NR2B subunit and study of ERK activation in neurons with varied content of NR2B suggests that NR2B-containing channels are the dominant activators of the NMDA-dependent ERK pathway... - The voltage-gated proton channel Hv1 enhances brain damage from ischemic strokeLong Jun Wu
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, USA
Nat Neurosci 15:565-73. 2012.... - TRP ion channels in the nervous systemMagdalene M Moran
Department of Cardiology, Harvard Medical School, 1309 Enders Building, 320 Longwood Avenue, Children's Hospital, Boston, MA 02115, USA
Curr Opin Neurobiol 14:362-9. 2004..TRP channels are emerging as essential cellular switches that allow animals to respond to their environments... - TATA-binding protein (TBP)-like factor (TLF) is a functional regulator of transcription: reciprocal regulation of the neurofibromatosis type 1 and c-fos genes by TLF/TRF2 and TBPJayhong A Chong
Department of Cardiology, Children s Hospital, Enders 1309, 320 Longwood Ave, Boston, MA 02115, USA
Mol Cell Biol 25:2632-43. 2005..We conclude that TLF is a functional regulator of transcription with targets distinct from those of TBP... - Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic developmentHao Zhou
Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, and Harvard Medical School, 1309 Enders, 320 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 106:15750-5. 2009..We conclude that MagT1 and TUSC3 are indispensable members of the vertebrate plasma membrane Mg(2+) transport system... - Real-time imaging of nuclear permeation by EGFP in single intact cellsXunbin Wei
Howard Hughes Medical Institute, Children's Hospital, Boston, Massachusetts 02115, USA
Biophys J 84:1317-27. 2003..We also found that EGFP exchange was not altered significantly during the cell cycle... - Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burstI Scott Ramsey
Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, and Department of Cardiovascular Research, Manton Center for Orphan Disease, Children s Hospital, 320 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 106:7642-7. 2009..Despite significant reduction in superoxide production, Hv1(-/-) mice are able to clear several types of bacterial infections... - Essential role for TRPC5 in amygdala function and fear-related behaviorAntonio Riccio
Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, MA 02115, USA
Cell 137:761-72. 2009..These experiments provide genetic evidence that TRPC5, activated via G protein-coupled neuronal receptors, has an essential function in innate fear... - Functional TRPM7 channels accumulate at the plasma membrane in response to fluid flowElena Oancea
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
Circ Res 98:245-53. 2006..In vascular smooth muscle cells, but not in several types of endothelial cells, fluid flow increased endogenous native TRPM7 current amplitude. We hypothesize that TRPM7 plays a role in pathological response to vessel wall injury... - An introduction to TRP channelsI Scott Ramsey
Howard Hughes Medical Institute, Cardiovascular Department, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
Annu Rev Physiol 68:619-47. 2006..The TRP channel gene family and its nomenclature, the encoded proteins and alternatively spliced variants, and the rapidly expanding pharmacology of TRP channels are summarized in online supplemental material... - CACNA1H mutations in autism spectrum disordersIgor Splawski
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Boston, Massachusetts 02115, USA
J Biol Chem 281:22085-91. 2006..2 channel activity and thus could affect neuronal function and potentially brain development. We conclude that the identified mutations could contribute to the development of the ASD phenotype... - The cation selectivity filter of the bacterial sodium channel, NaChBacLixia Yue
Howard Hughes Medical Institute, Children's Hospital, Boston, MA 02115, USA
J Gen Physiol 120:845-53. 2002..When additional aspartates were incorporated (LDDWAD), the mutant channel resulted in a highly expressing voltage-gated Ca(2+)-selective conductance... - Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heartSean M Wu
Division of Hematology/Oncology, Children's Hospital, Boston, MA 02115, USA
Cell 127:1137-50. 2006..5(+) cells from mouse embryos and demonstrated their capacity for bipotential differentiation in vivo. Taken together, these results support the existence of a common precursor for cardiovascular lineages in the mammalian heart... - TRPV4 is a regulator of adipose oxidative metabolism, inflammation, and energy homeostasisLi Ye
Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
Cell 151:96-110. 2012..This role of TRPV4 as a cell-autonomous mediator for both the thermogenic and proinflammatory programs in adipocytes could offer a target for treating obesity and related metabolic diseases... - Targeted cytosolic delivery of cell-impermeable compounds by nanoparticle-mediated, light-triggered endosome disruptionSébastien Febvay
Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, Massachusetts 02115, USA
Nano Lett 10:2211-9. 2010..As an example, we delivered a cell-impermeable fluorescent compound exclusively to the cytosol of multidrug resistant cancer cells in a mixed population... - Activating mutation in a mucolipin transient receptor potential channel leads to melanocyte loss in varitint-waddler miceHaoxing Xu
Department of Cardiology, Howard Hughes Medical Institute, Children s Hospital Boston, Harvard Medical School, Enders Building 1309, 320 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 104:18321-6. 2007..We conclude that the Va phenotype is caused by mutation-induced TRPML3 gain-of-function, resulting in cell death... - International Union of Basic and Clinical Pharmacology. LXXVI. Current progress in the mammalian TRP ion channel familyLong Jun Wu
Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston, 320 Longwood Avenue, Boston, MA 02115, USA
Pharmacol Rev 62:381-404. 2010..Here we review the recent progress on the physiology, pharmacology and pathophysiological function of mammalian TRP channels... - Mechanism of persistent protein kinase D1 translocation and activationElena Oancea
HHMI, Children's Hospital, Harvard Medical School, 1309 Enders Building, 320 Longwood Avenue, Boston, MA 02115, USA
Dev Cell 4:561-74. 2003..We incorporate the kinetics of PKD1 translocation into a three-state model that suggests how PKD1 binding to DAG and Galphaq uniquely encodes frequency-dependent PKD1 signaling... - TRPM7, the Mg(2+) inhibited channel and kinaseChris Bates-Withers
Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, 02115 Boston, MA, USA
Adv Exp Med Biol 704:173-83. 2011..In disease states, TRPM7 has been linked to Guamanian amyotrophic lateral sclerosis and parkinsonian dementia (ALS/PD), various forms of neoplasia, hypertension and delayed neuronal death following cerebral ischemia... - Anion-sensitive fluorophore identifies the Drosophila swell-activated chloride channel in a genome-wide RNA interference screenStephanie C Stotz
Howard Hughes Medical Institute, Department of Cardiology, Boston Children s Hospital, Boston, Massachusetts, United States of America
PLoS ONE 7:e46865. 2012..dBest1 is an anion channel clearly responsive to swell; this activation depends upon its N-terminus... - TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal functionJochen Reiser
Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02129, USA
Nat Genet 37:739-44. 2005..Two of the TRPC6 mutants had increased current amplitudes. These data show that TRPC6 channel activity at the slit diaphragm is essential for proper regulation of podocyte structure and function... - Modified herpes simplex virus delivery of enhanced GFP into the central nervous systemVladislav M Sandler
HHMI, Children's Hospital, Boston, MA, USA
J Neurosci Methods 121:211-9. 2002..We conclude that the HSV-1 amplicon is a valuable tool for gene delivery in the rat central nervous system... - The TRPM7 channel is inactivated by PIP(2) hydrolysisLoren W Runnels
Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, MA 02115, USA
Nat Cell Biol 4:329-36. 2002..We conclude that receptor-mediated activation of PLC results in the hydrolysis of localized PIP(2), leading to inactivation of the TRPM7 channel... - Calbindin-D28K dynamically controls TRPV5-mediated Ca2+ transportTim T Lambers
Department of Physiology, Radboud University Nijmegen Medical Centre, The Netherlands
EMBO J 25:2978-88. 2006..In summary, CaBP(28K) acts in Ca(2+)-transporting epithelia as a dynamic Ca(2+) buffer, regulating [Ca(2+)] in close vicinity to the TRPV5 pore by direct association with the channel... - CatSper1 required for evoked Ca2+ entry and control of flagellar function in spermAnne E Carlson
Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
Proc Natl Acad Sci U S A 100:14864-8. 2003..We propose that CatSper1 functions as a voltage-gated Ca2+ channel that controls Ca2+ entry to mediate the hyperactivated motility needed late in the preparation of sperm for fertilization... - A unified nomenclature for the superfamily of TRP cation channelsCraig Montell
Mol Cell 9:229-31. 2002 - International Union of Pharmacology. LIV. Nomenclature and molecular relationships of inwardly rectifying potassium channelsYoshihiro Kubo
Division of Biophysics and Neurobiology, Department of Molecular Physiology, National Institute for Physiological Sciences, Nishigoh-naka 38, Myodaiji, Okazaki, Aichi 444-8585, Japan
Pharmacol Rev 57:509-26. 2005 - CatSperbeta, a novel transmembrane protein in the CatSper channel complexJin Liu
Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
J Biol Chem 282:18945-52. 2007..CatSperbeta protein is absent in CatSper1(-/-) sperm, suggesting that it is required for trafficking or formation of a stable channel complex. CatSperbeta is the first identified auxiliary protein to the CatSper channel... - The voltage-gated Na+ channel NaVBP has a role in motility, chemotaxis, and pH homeostasis of an alkaliphilic BacillusMasahiro Ito
Faculty of Life Sciences, Toyo University, Oura-gun, Gunma 374-0193, Japan
Proc Natl Acad Sci U S A 101:10566-71. 2004..We hypothesize that activation of Na(V)BP at high pH supports diverse physiological processes by a combination of direct and indirect effects on the Na(+) cycle and the chemotaxis system...
Research Grants
- Canonical Transient Receptor Potential (TRPC) subfamily function in Hippocampus.David E Clapham; Fiscal Year: 2010....