David E Clapham

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Sorting out MIC, TRP, and CRAC ion channels
    David E Clapham
    Howard Hughes Medical Institute Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Gen Physiol 120:217-20. 2002
  2. pmc Citral sensing by Transient [corrected] receptor potential channels in dorsal root ganglion neurons
    Stephanie C Stotz
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 3:e2082. 2008
  3. pmc Anion-sensitive fluorophore identifies the Drosophila swell-activated chloride channel in a genome-wide RNA interference screen
    Stephanie C Stotz
    Howard Hughes Medical Institute, Department of Cardiology, Boston Children s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 7:e46865. 2012
  4. pmc Controlled delivery of bioactive molecules into live cells using the bacterial mechanosensitive channel MscL
    Julia F Doerner
    HHMI, Department of Cardiology, Boston Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Commun 3:990. 2012
  5. pmc Intracellular calcium strongly potentiates agonist-activated TRPC5 channels
    Nathaniel T Blair
    Howard Hughes Medical Institute, Department of Cardiology and Manton Center for Orphan Disease, Children s Hospital Boston, and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    J Gen Physiol 133:525-46. 2009
  6. pmc MCU encodes the pore conducting mitochondrial calcium currents
    Dipayan Chaudhuri
    Cardiovascular Research Center, Massachusetts General Hospital, Boston, United States Department of Cardiology, Howard Hughes Medical Institute, Boston Children s Hospital, Boston, United States
    elife 2:e00704. 2013
  7. ncbi Symmetry, selectivity, and the 2003 Nobel Prize
    David E Clapham
    Howard Hughes Medical Institute, Children s Hospital of Boston, Boston, MA 02115, USA
    Cell 115:641-6. 2003
  8. ncbi International Union of Pharmacology. L. Nomenclature and structure-function relationships of CatSper and two-pore channels
    David E Clapham
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115, USA
    Pharmacol Rev 57:451-4. 2005
  9. ncbi International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels
    David E Clapham
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115, USA
    Pharmacol Rev 57:427-50. 2005
  10. ncbi TRP channels as cellular sensors
    David E Clapham
    Howard Hughes Medical Institute, Pediatric Cardiology, Children s Hospital of Boston, Department of Neurobiology, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 426:517-24. 2003

Collaborators

Detail Information

Publications81

  1. pmc Sorting out MIC, TRP, and CRAC ion channels
    David E Clapham
    Howard Hughes Medical Institute Children s Hospital, Harvard Medical School, Boston, MA 02115, USA
    J Gen Physiol 120:217-20. 2002
  2. pmc Citral sensing by Transient [corrected] receptor potential channels in dorsal root ganglion neurons
    Stephanie C Stotz
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 3:e2082. 2008
    ..Citral's broad spectrum and prolonged sensory inhibition may prove more useful than capsaicin for allodynia, itch, or other types of pain involving superficial sensory nerves and skin...
  3. pmc Anion-sensitive fluorophore identifies the Drosophila swell-activated chloride channel in a genome-wide RNA interference screen
    Stephanie C Stotz
    Howard Hughes Medical Institute, Department of Cardiology, Boston Children s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 7:e46865. 2012
    ..dBest1 is an anion channel clearly responsive to swell; this activation depends upon its N-terminus...
  4. pmc Controlled delivery of bioactive molecules into live cells using the bacterial mechanosensitive channel MscL
    Julia F Doerner
    HHMI, Department of Cardiology, Boston Children s Hospital, Boston, Massachusetts 02115, USA
    Nat Commun 3:990. 2012
    ..We propose that MscL will be a useful tool for gated and controlled delivery of bioactive molecules into cells...
  5. pmc Intracellular calcium strongly potentiates agonist-activated TRPC5 channels
    Nathaniel T Blair
    Howard Hughes Medical Institute, Department of Cardiology and Manton Center for Orphan Disease, Children s Hospital Boston, and Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    J Gen Physiol 133:525-46. 2009
    ..Ca(2+)-dependent potentiation should result in TRPC5 channel activation preferentially during periods of repetitive firing or coincident neurotransmitter receptor activation...
  6. pmc MCU encodes the pore conducting mitochondrial calcium currents
    Dipayan Chaudhuri
    Cardiovascular Research Center, Massachusetts General Hospital, Boston, United States Department of Cardiology, Howard Hughes Medical Institute, Boston Children s Hospital, Boston, United States
    elife 2:e00704. 2013
    ..These analyses establish that MCU encodes the pore-forming subunit of the uniporter channel. DOI:http://dx.doi.org/10.7554/eLife.00704.001...
  7. ncbi Symmetry, selectivity, and the 2003 Nobel Prize
    David E Clapham
    Howard Hughes Medical Institute, Children s Hospital of Boston, Boston, MA 02115, USA
    Cell 115:641-6. 2003
  8. ncbi International Union of Pharmacology. L. Nomenclature and structure-function relationships of CatSper and two-pore channels
    David E Clapham
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115, USA
    Pharmacol Rev 57:451-4. 2005
  9. ncbi International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels
    David E Clapham
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115, USA
    Pharmacol Rev 57:427-50. 2005
  10. ncbi TRP channels as cellular sensors
    David E Clapham
    Howard Hughes Medical Institute, Pediatric Cardiology, Children s Hospital of Boston, Department of Neurobiology, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 426:517-24. 2003
    ..They are an ancient sensory apparatus for the cell, not just the multicellular organism, and they have been adapted to respond to all manner of stimuli, from both within and outside the cell...
  11. ncbi Signal transduction. Hot and cold TRP ion channels
    David E Clapham
    Howard Hughes Medical Institute, Cardiovascular Division, Children s Hospital, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA
    Science 295:2228-9. 2002
  12. ncbi Calcium signaling
    David E Clapham
    Aldo R Castañeda Professor of Cardiovascular Research, Professor of Neurobiology, Harvard Medical School, Howard Hughes Medical Institute, Enders 1309, Children s Hospital Boston, Boston, MA 02115, USA
    Cell 131:1047-58. 2007
    ..Also discussed is the highly localized nature of Ca(2+)-mediated signal transduction and its specific roles in excitability, exocytosis, motility, apoptosis, and transcription...
  13. pmc A thermodynamic framework for understanding temperature sensing by transient receptor potential (TRP) channels
    David E Clapham
    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:19492-7. 2011
    ..This postulate, along with the laws of thermodynamics and independent of mechanistic detail, leads to the conclusion that hot- and cold-sensing TRPs operate by identical conformational changes...
  14. ncbi Formation of novel TRPC channels by complex subunit interactions in embryonic brain
    Carsten Strübing
    Howard Hughes Medical Institute, Department of Cardiovascular Research, Children s Hospital and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:39014-9. 2003
    ..This complex assembly mechanism increases the diversity of TRPC channels in mammalian brain and may generate novel heteromers that have specific roles in the developing brain...
  15. ncbi Rapid vesicular translocation and insertion of TRP channels
    Vassilios J Bezzerides
    Howard Hughes Medical Institute, Cardiovascular Department, Children s Hospital and Harvard Medical School, Enders 1309 320 Longwood Avenue, Boston, MA 02115, USA
    Nat Cell Biol 6:709-20. 2004
    ..The increase in TRPC5 availability affects neurite extension rates in cultured hippocampal neurons, and may be a general mechanism for initiating Ca(2+) influx and cell morphological changes in response to stimuli...
  16. pmc TRPM1 forms ion channels associated with melanin content in melanocytes
    Elena Oancea
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
    Sci Signal 2:ra21. 2009
    ..We propose that TRPM1 is an ion channel whose function is critical to normal melanocyte pigmentation and is thus a potential target for pigmentation disorders...
  17. ncbi The TRPM7 ion channel functions in cholinergic synaptic vesicles and affects transmitter release
    Grigory Krapivinsky
    Howard Hughes Medical Institute, Cardiology, Children s Hospital Boston, 1309 Enders Building, 320 Longwood Avenue, Boston, MA, USA
    Neuron 52:485-96. 2006
    ..Targeted peptide interference of TRPM7's interaction with snapin affects the amplitudes and kinetics of postsynaptic EPSPs. Thus, vesicular TRPM7 channel activity is critical to neurotransmitter release in sympathetic neurons...
  18. pmc Ion channel-kinase TRPM7 is required for maintaining cardiac automaticity
    Rajan Sah
    Howard Hughes Medical Institute, Department of Cardiology, Boston Children s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:E3037-46. 2013
    ..We conclude that TRPM7 influences diastolic membrane depolarization and automaticity in SAN indirectly via regulation of Hcn4 expression...
  19. pmc Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter
    Dawei Jiang
    Department of Cardiology, Howard Hughes Medical Institute, Children s Hospital Boston, Manton Center for Orphan Disease, and Department of Neurobiology, Harvard Medical School, Enders Building 1309, 320 Longwood Avenue, Boston, MA 02115, USA
    Science 326:144-7. 2009
    ..RNAi knockdown, overexpression, and liposome reconstitution of the purified Letm1 protein demonstrate that Letm1 is a mitochondrial Ca2+/H+ antiporter...
  20. ncbi The mitochondrial calcium uniporter is a highly selective ion channel
    Yuriy Kirichok
    Howard Hughes Medical Institute, Department of Cardiovascular Research, Children s Hospital, andHarvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 427:360-4. 2004
    ..The channel is inwardly rectifying, making it especially effective for Ca2+ uptake into energized mitochondria. Thus, we conclude that the properties of the current mediated by this novel channel are those of the MCU...
  21. pmc All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility
    Huayu Qi
    Department of Cardiology, Howard Hughes Medical Institute, and Department of Neurobiology, Harvard Medical School, Enders 1309, Children s Hospital Boston, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:1219-23. 2007
    ..Direct protein interactions among CatSpers, the sperm specificity of these proteins, and loss of ICatSper in each of the four CatSper-/- mice indicate that CatSpers are highly specialized flagellar proteins...
  22. pmc Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome
    Dawei Jiang
    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 110:E2249-54. 2013
    ..We conclude that loss of Letm1 contributes to the pathology of Wolf-Hirschhorn syndrome in humans and may contribute to seizure phenotypes by reducing glucose oxidation and other specific metabolic alterations...
  23. pmc Cleavage of TRPM7 releases the kinase domain from the ion channel and regulates its participation in Fas-induced apoptosis
    Bimal N Desai
    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, and Department of Neurobiology, Harvard Medical School, Enders Building 1309, 320 Longwood Avenue, Boston, MA 02115, USA
    Dev Cell 22:1149-62. 2012
    ..These findings raise the possibility that other members of the TRP channel superfamily are also regulated by caspase-mediated cleavage, with wide-ranging implications for cell death and differentiation...
  24. pmc POST, partner of stromal interaction molecule 1 (STIM1), targets STIM1 to multiple transporters
    Grigory Krapivinsky
    Department of Cardiology, The Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:19234-9. 2011
    ..Store depletion promotes STIM1-POST complex binding to smooth ER and plasma membrane Ca(2+) ATPases (SERCAs and PMCAs, respectively), Na/K-ATPase, as well as to the nuclear transporters, importins-β and exportins...
  25. ncbi SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation
    Grigory Krapivinsky
    Howard Hughes Medical Institute, Children s Hospital, 1309 Enders Building, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Neuron 43:563-74. 2004
    ..siRNA-mediated SynGAP knockdown confirmed these results. These data implicate SynGAP in NMDAR- and CaMKII-dependent regulation of AMPAR trafficking...
  26. ncbi Camphor activates and strongly desensitizes the transient receptor potential vanilloid subtype 1 channel in a vanilloid-independent mechanism
    Haoxing Xu
    Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Neurosci 25:8924-37. 2005
    ..We also found that camphor inhibited several other related TRP channels, including ankyrin-repeat TRP 1 (TRPA1). The camphor-induced desensitization of TRPV1 and block of TRPA1 may underlie the analgesic effects of camphor...
  27. ncbi Phosphatidylinositol 3-kinase activates ERK in primary sensory neurons and mediates inflammatory heat hyperalgesia through TRPV1 sensitization
    Zhi Ye Zhuang
    Pain Research Center, Department of Anesthesiology, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
    J Neurosci 24:8300-9. 2004
    ..Therefore, PI3K induces heat hyperalgesia, possibly by regulating TRPV1 activity, in an ERK-dependent manner. The PI3K pathway also appears to play a role that is distinct from ERK by regulating the early onset of inflammatory pain...
  28. ncbi TRPC5 is a regulator of hippocampal neurite length and growth cone morphology
    Anna Greka
    Howard Hughes Medical Institute, Children s Hospital and Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Neurosci 6:837-45. 2003
    ..Dominant-negative TRPC5 expression allowed significantly longer neurites and filopodia to form. We conclude that TRPC5 channels are important components of the mechanism controlling neurite extension and growth cone morphology...
  29. ncbi Oregano, thyme and clove-derived flavors and skin sensitizers activate specific TRP channels
    Haoxing Xu
    Howard Hughes Medical Institute, Harvard Medical School, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Neurosci 9:628-35. 2006
    ..Our results support a role for temperature-sensitive TRP channels in chemesthesis in oral and nasal epithelium and suggest that TRPV3 may be a molecular target of plant-derived skin sensitizers...
  30. pmc TRPM7 facilitates cholinergic vesicle fusion with the plasma membrane
    Sebastian Brauchi
    Department of Cardiology, Howard Hughes Medical Institute, Children s Hospital Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 105:8304-8. 2008
    ..We conclude that the conductance of TRPM7 across the vesicle membrane is important in SSLV fusion...
  31. pmc The channel kinase, TRPM7, is required for early embryonic development
    Jie Jin
    Department of Cardiology, Manton Center for Orphan Disease, Children s Hospital Boston, Howard Hughes Medical Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 109:E225-33. 2012
    ..The in vivo and in vitro results demonstrate a temporal requirement for the Trpm7 channel kinase during embryogenesis...
  32. pmc A voltage-gated proton-selective channel lacking the pore domain
    I Scott Ramsey
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 440:1213-6. 2006
    ..The data presented here identify H(v)1 as a long-sought voltage-gated H+ channel and establish H(v)1 as the founding member of a family of mammalian VSD proteins...
  33. ncbi A superfamily of voltage-gated sodium channels in bacteria
    Ryuta Koishi
    Howard Hughes Medical Institute, Department of Cardiovascular Research, Children s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 279:9532-8. 2004
    ..Thus, the Na(V)Bac superfamily is fertile ground for crystallographic, electrophysiological, and microbiological studies...
  34. pmc Decreased anxiety-like behavior and Gαq/11-dependent responses in the amygdala of mice lacking TRPC4 channels
    Antonio Riccio
    Howard Hughes Medical Institute, Department of Cardiology, Boston Children s Hospital, Boston, Massachusetts 02115, Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, Department of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, Massachusetts 02478, and Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St Petersburg 194223, Russia
    J Neurosci 34:3653-67. 2014
    ..Thus, TRPC4 and the structurally and functionally related subunit, TRPC5, may both contribute to the mechanisms underlying regulation of innate fear responses. ..
  35. ncbi TRPV3 is a calcium-permeable temperature-sensitive cation channel
    Haoxing Xu
    Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 418:181-6. 2002
    ..On the basis of these properties, we propose that hTRPV3 is thermosensitive in the physiological range of temperatures between TRPM8 and TRPV1...
  36. ncbi Primary cilia are specialized calcium signalling organelles
    Markus Delling
    1 Howard Hughes Medical Institute, Department of Cardiology, Boston Children s Hospital, 320 Longwood Avenue, Boston, Massachusetts 02115, USA 2
    Nature 504:311-4. 2013
    ..PKD1L1-PKD2L1 acts as a ciliary calcium channel controlling ciliary calcium concentration and thereby modifying SMO-activated GLI2 translocation and GLI1 expression. ..
  37. pmc KSper, a pH-sensitive K+ current that controls sperm membrane potential
    Betsy Navarro
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:7688-92. 2007
    ..Alkalinization activates the pH(i)-sensitive I(KSper), setting the membrane potential to negative potentials where Ca2+ entry via I(CatSper) is maximized. I(KSper) is one of two dominant ion currents of capacitated sperm cells...
  38. doi Ion channels that control fertility in mammalian spermatozoa
    Betsy Navarro
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Department of Neurobiology, Harvard Medical School, Enders 1309, Children s Hospital Boston, 320 Longwood Avenue, Boston, MA 02115, USA
    Int J Dev Biol 52:607-13. 2008
    ..Intracellular alkalinization activates both channels and induces hyperactivated motility...
  39. ncbi Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel
    Yuriy Kirichok
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital and Harvard Medical School Enders 1309, Boston, Massachusetts 02115, USA
    Nature 439:737-40. 2006
    ..Our results identify CatSper1 as a component of the key flagellar calcium channel, and suggest that intracellular alkalinization potentiates CatSper current to increase intraflagellar calcium and induce sperm hyperactivation...
  40. pmc ATP-activated P2X2 current in mouse spermatozoa
    Betsy Navarro
    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:14342-7. 2011
    ..However, fertility of P2rx2(-/-) males declines with frequent mating over days, suggesting that P2X2 receptor adds a selection advantage under these conditions...
  41. pmc EMRE is an essential component of the mitochondrial calcium uniporter complex
    Yasemin Sancak
    Department of Molecular Biology, Massachusetts General Hospital, Department of Systems Biology, Harvard Medical School, Boston, MA, USA
    Science 342:1379-82. 2013
    ..EMRE was required for the interaction of MCU with MICU1 and MICU2. Hence, EMRE is essential for in vivo uniporter current and additionally bridges the calcium-sensing role of MICU1 and MICU2 with the calcium-conducting role of MCU. ..
  42. pmc A spontaneous, recurrent mutation in divalent metal transporter-1 exposes a calcium entry pathway
    Haoxing Xu
    Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Biol 2:E50. 2004
    ..Here we show that G185R mutant DMT1 exhibits a new, constitutive Ca(2+) permeability, suggesting a gain of function that contributes to remutation and the mk and b phenotypes...
  43. pmc Transient receptor potential cation channel, subfamily C, member 5 (TRPC5) is a cold-transducer in the peripheral nervous system
    Katharina Zimmermann
    Department of Cardiology, Manton Center for Orphan Disease, and Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 108:18114-9. 2011
    ..These findings demonstrate that highly cold-sensitive TRPC5 channels are a molecular component for detection and regional adaptation to cold temperatures in the peripheral nervous system that is distinct from noxious cold sensing...
  44. pmc Deletion of Trpm7 disrupts embryonic development and thymopoiesis without altering Mg2+ homeostasis
    Jie Jin
    Department of Cardiology, Howard Hughes Medical Institute, Children s Hospital Boston, Boston, MA 02115, USA
    Science 322:756-60. 2008
    ..The thymic medullary cells lost signal transducer and activator of transcription 3 activity, which accounts for their depletion when Trpm7 is disrupted in thymocytes...
  45. ncbi Mechanism of persistent protein kinase D1 translocation and activation
    Elena Oancea
    HHMI, Children s Hospital, Harvard Medical School, 1309 Enders Building, 320 Longwood Avenue, Boston, MA 02115, USA
    Dev Cell 4:561-74. 2003
    ..We incorporate the kinetics of PKD1 translocation into a three-state model that suggests how PKD1 binding to DAG and Galphaq uniquely encodes frequency-dependent PKD1 signaling...
  46. pmc Real-time imaging of nuclear permeation by EGFP in single intact cells
    Xunbin Wei
    Howard Hughes Medical Institute, Children s Hospital, Boston, Massachusetts 02115, USA
    Biophys J 84:1317-27. 2003
    ..We also found that EGFP exchange was not altered significantly during the cell cycle...
  47. pmc Direct recording and molecular identification of the calcium channel of primary cilia
    Paul G DeCaen
    1 Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston, 320 Longwood Avenue, Boston, Massachusetts 02115, USA 2
    Nature 504:315-8. 2013
    ..In conjunction with an accompanying manuscript, we show that the PKD1L1-PKD2L1 heteromeric channel establishes the cilia as a unique calcium compartment within cells that modulates established hedgehog pathways. ..
  48. pmc An aqueous H+ permeation pathway in the voltage-gated proton channel Hv1
    I Scott Ramsey
    Howard Hughes Medical Institute, Department of Cardiology and Manton Center for Orphan Disease, Children s Hospital Boston, Boston, Massachusetts, USA
    Nat Struct Mol Biol 17:869-75. 2010
    ..Our results indicate that Hv1 most likely forms an internal water wire for selective proton transfer and that interactions between water molecules and S4 arginines may underlie coupling between voltage- and pH-gradient sensing...
  49. pmc Timing of myocardial trpm7 deletion during cardiogenesis variably disrupts adult ventricular function, conduction, and repolarization
    Rajan Sah
    Howard Hughes Medical Institute, Department of Cardiology, Manton Center for Orphan Disease, Children s Hospital Boston, 320 Longwood Ave, Enders 1309, Boston, MA 02115, USA
    Circulation 128:101-14. 2013
    ..TRPM7 is a divalent-permeant channel and kinase of unknown function, recently implicated in the pathogenesis of atrial fibrillation; however, its function in ventricular myocardium remains unexplored...
  50. ncbi Developmental origin of a bipotential myocardial and smooth muscle cell precursor in the mammalian heart
    Sean M Wu
    Division of Hematology Oncology, Children s Hospital, Boston, MA 02115, USA
    Cell 127:1137-50. 2006
    ..5(+) cells from mouse embryos and demonstrated their capacity for bipotential differentiation in vivo. Taken together, these results support the existence of a common precursor for cardiovascular lineages in the mammalian heart...
  51. pmc The cation selectivity filter of the bacterial sodium channel, NaChBac
    Lixia Yue
    Howard Hughes Medical Institute, Children s Hospital, Boston, MA 02115, USA
    J Gen Physiol 120:845-53. 2002
    ..When additional aspartates were incorporated (LDDWAD), the mutant channel resulted in a highly expressing voltage-gated Ca(2+)-selective conductance...
  52. pmc TATA-binding protein (TBP)-like factor (TLF) is a functional regulator of transcription: reciprocal regulation of the neurofibromatosis type 1 and c-fos genes by TLF/TRF2 and TBP
    Jayhong A Chong
    Department of Cardiology, Children s Hospital, Enders 1309, 320 Longwood Ave, Boston, MA 02115, USA
    Mol Cell Biol 25:2632-43. 2005
    ..We conclude that TLF is a functional regulator of transcription with targets distinct from those of TBP...
  53. pmc Rheotaxis guides mammalian sperm
    Kiyoshi Miki
    Howard Hughes Medical Institute, Department of Cardiology, Manton Center for Orphan Disease Research, Boston Children s Hospital, 320 Longwood Avenue, Enders 1309, Boston, MA 02115, USA
    Curr Biol 23:443-52. 2013
    ..Currently, chemotaxis, thermotaxis, and rheotaxis have not been definitively established in mammals...
  54. pmc The voltage-gated proton channel Hv1 enhances brain damage from ischemic stroke
    Long Jun Wu
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston and Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, USA
    Nat Neurosci 15:565-73. 2012
    ....
  55. pmc Calpain cleaves and activates the TRPC5 channel to participate in semaphorin 3A-induced neuronal growth cone collapse
    J Stefan Kaczmarek
    Program in Neuroscience, Division of Medical Sciences, Harvard University, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 109:7888-92. 2012
    ..Thus, TRPC5 acts downstream of semaphorin signaling to cause changes in neuronal growth cone morphology and nervous system development...
  56. ncbi The NMDA receptor is coupled to the ERK pathway by a direct interaction between NR2B and RasGRF1
    Grigory Krapivinsky
    Howard Hughes Medical Institute, Children s Hospital, 1309 Enders Building, 320 Longwood Avenue, Boston, MA 02115, USA
    Neuron 40:775-84. 2003
    ..The specific association of RasGRF1 with the NR2B subunit and study of ERK activation in neurons with varied content of NR2B suggests that NR2B-containing channels are the dominant activators of the NMDA-dependent ERK pathway...
  57. ncbi International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels
    David E Clapham
    Howard Hughes Medical Institute, Children s Hospital, 1309 Enders Building, 320 Longwood Ave, Boston, MA 02115, USA
    Pharmacol Rev 55:591-6. 2003
    ..The complete Compendium, including data tables for each member of the TRP channel family, can be found at http://www.iuphar-db.org/iuphar-ic/...
  58. pmc TRPV4 is a regulator of adipose oxidative metabolism, inflammation, and energy homeostasis
    Li Ye
    Department of Cancer Biology, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Cell 151:96-110. 2012
    ..This role of TRPV4 as a cell-autonomous mediator for both the thermogenic and proinflammatory programs in adipocytes could offer a target for treating obesity and related metabolic diseases...
  59. ncbi Functional TRPM7 channels accumulate at the plasma membrane in response to fluid flow
    Elena Oancea
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston, Harvard Medical School, Boston, MA 02115, USA
    Circ Res 98:245-53. 2006
    ..In vascular smooth muscle cells, but not in several types of endothelial cells, fluid flow increased endogenous native TRPM7 current amplitude. We hypothesize that TRPM7 plays a role in pathological response to vessel wall injury...
  60. ncbi The TRPM7 channel is inactivated by PIP(2) hydrolysis
    Loren W Runnels
    Howard Hughes Medical Institute, Children s Hospital, Harvard Medical School, Enders 1309, 320 Longwood Avenue, Boston, MA 02115, USA
    Nat Cell Biol 4:329-36. 2002
    ..We conclude that receptor-mediated activation of PLC results in the hydrolysis of localized PIP(2), leading to inactivation of the TRPM7 channel...
  61. ncbi CACNA1H mutations in autism spectrum disorders
    Igor Splawski
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital, Boston, Massachusetts 02115, USA
    J Biol Chem 281:22085-91. 2006
    ..2 channel activity and thus could affect neuronal function and potentially brain development. We conclude that the identified mutations could contribute to the development of the ASD phenotype...
  62. pmc Mammalian MagT1 and TUSC3 are required for cellular magnesium uptake and vertebrate embryonic development
    Hao Zhou
    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, and Harvard Medical School, 1309 Enders, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:15750-5. 2009
    ..We conclude that MagT1 and TUSC3 are indispensable members of the vertebrate plasma membrane Mg(2+) transport system...
  63. ncbi TRP ion channels in the nervous system
    Magdalene M Moran
    Department of Cardiology, Harvard Medical School, 1309 Enders Building, 320 Longwood Avenue, Children s Hospital, Boston, MA 02115, USA
    Curr Opin Neurobiol 14:362-9. 2004
    ..TRP channels are emerging as essential cellular switches that allow animals to respond to their environments...
  64. pmc Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst
    I Scott Ramsey
    Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, and Department of Cardiovascular Research, Manton Center for Orphan Disease, Children s Hospital, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:7642-7. 2009
    ..Despite significant reduction in superoxide production, Hv1(-/-) mice are able to clear several types of bacterial infections...
  65. ncbi An introduction to TRP channels
    I Scott Ramsey
    Howard Hughes Medical Institute, Cardiovascular Department, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    Annu Rev Physiol 68:619-47. 2006
    ..The TRP channel gene family and its nomenclature, the encoded proteins and alternatively spliced variants, and the rapidly expanding pharmacology of TRP channels are summarized in online supplemental material...
  66. pmc Essential role for TRPC5 in amygdala function and fear-related behavior
    Antonio Riccio
    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, MA 02115, USA
    Cell 137:761-72. 2009
    ..These experiments provide genetic evidence that TRPC5, activated via G protein-coupled neuronal receptors, has an essential function in innate fear...
  67. doi TRPM7, the Mg(2+) inhibited channel and kinase
    Chris Bates-Withers
    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, 02115 Boston, MA, USA
    Adv Exp Med Biol 704:173-83. 2011
    ..In disease states, TRPM7 has been linked to Guamanian amyotrophic lateral sclerosis and parkinsonian dementia (ALS/PD), various forms of neoplasia, hypertension and delayed neuronal death following cerebral ischemia...
  68. pmc Activating mutation in a mucolipin transient receptor potential channel leads to melanocyte loss in varitint-waddler mice
    Haoxing Xu
    Department of Cardiology, Howard Hughes Medical Institute, Children s Hospital Boston, Harvard Medical School, Enders Building 1309, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 104:18321-6. 2007
    ..We conclude that the Va phenotype is caused by mutation-induced TRPML3 gain-of-function, resulting in cell death...
  69. pmc International Union of Basic and Clinical Pharmacology. LXXVI. Current progress in the mammalian TRP ion channel family
    Long Jun Wu
    Howard Hughes Medical Institute, Department of Cardiology, Children s Hospital Boston, 320 Longwood Avenue, Boston, MA 02115, USA
    Pharmacol Rev 62:381-404. 2010
    ..Here we review the recent progress on the physiology, pharmacology and pathophysiological function of mammalian TRP channels...
  70. pmc Targeted cytosolic delivery of cell-impermeable compounds by nanoparticle-mediated, light-triggered endosome disruption
    Sébastien Febvay
    Department of Cardiology, Howard Hughes Medical Institute, Manton Center for Orphan Disease, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Nano Lett 10:2211-9. 2010
    ..As an example, we delivered a cell-impermeable fluorescent compound exclusively to the cytosol of multidrug resistant cancer cells in a mixed population...
  71. pmc TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function
    Jochen Reiser
    Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02129, USA
    Nat Genet 37:739-44. 2005
    ..Two of the TRPC6 mutants had increased current amplitudes. These data show that TRPC6 channel activity at the slit diaphragm is essential for proper regulation of podocyte structure and function...
  72. ncbi Modified herpes simplex virus delivery of enhanced GFP into the central nervous system
    Vladislav M Sandler
    HHMI, Children s Hospital, Boston, MA, USA
    J Neurosci Methods 121:211-9. 2002
    ..We conclude that the HSV-1 amplicon is a valuable tool for gene delivery in the rat central nervous system...
  73. pmc Calbindin-D28K dynamically controls TRPV5-mediated Ca2+ transport
    Tim T Lambers
    Department of Physiology, Radboud University Nijmegen Medical Centre, The Netherlands
    EMBO J 25:2978-88. 2006
    ..In summary, CaBP(28K) acts in Ca(2+)-transporting epithelia as a dynamic Ca(2+) buffer, regulating [Ca(2+)] in close vicinity to the TRPV5 pore by direct association with the channel...
  74. ncbi International Union of Pharmacology. LIV. Nomenclature and molecular relationships of inwardly rectifying potassium channels
    Yoshihiro Kubo
    Division of Biophysics and Neurobiology, Department of Molecular Physiology, National Institute for Physiological Sciences, Nishigoh naka 38, Myodaiji, Okazaki, Aichi 444 8585, Japan
    Pharmacol Rev 57:509-26. 2005
  75. pmc The voltage-gated Na+ channel NaVBP has a role in motility, chemotaxis, and pH homeostasis of an alkaliphilic Bacillus
    Masahiro Ito
    Faculty of Life Sciences, Toyo University, Oura gun, Gunma 374 0193, Japan
    Proc Natl Acad Sci U S A 101:10566-71. 2004
    ..We hypothesize that activation of Na(V)BP at high pH supports diverse physiological processes by a combination of direct and indirect effects on the Na(+) cycle and the chemotaxis system...
  76. ncbi A unified nomenclature for the superfamily of TRP cation channels
    Craig Montell
    Mol Cell 9:229-31. 2002
  77. ncbi CatSperbeta, a novel transmembrane protein in the CatSper channel complex
    Jin Liu
    Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 282:18945-52. 2007
    ..CatSperbeta protein is absent in CatSper1(-/-) sperm, suggesting that it is required for trafficking or formation of a stable channel complex. CatSperbeta is the first identified auxiliary protein to the CatSper channel...
  78. pmc CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm
    Anne E Carlson
    Department of Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 100:14864-8. 2003
    ..We propose that CatSper1 functions as a voltage-gated Ca2+ channel that controls Ca2+ entry to mediate the hyperactivated motility needed late in the preparation of sperm for fertilization...

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