Research Topics
Genomes and Genes | John W ChenSummaryAffiliation: Harvard University Country: USA Publications
Research Grants
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Detail Information
Publications
Imaging of myeloperoxidase in mice by using novel amplifiable paramagnetic substratesJohn W Chen
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, 5404 Building 149, 13th St, Charlestown, MA 02129, USA
Radiology 240:473-81. 2006....- Vulnerable plaque imagingJohn W Chen
Division of Neuroradiology, Department of Radiology, Massachusetts General Hospital, Boston, MA, USA
Neuroimaging Clin N Am 15:609-21, xi. 2005..Although MR imaging, CT, and ultrasound provide data regarding single lesions, future investigations relying heavily on nuclear medicine techniques may offer functional assessment of the entire cardiovascular system... 
Myeloperoxidase-targeted imaging of active inflammatory lesions in murine experimental autoimmune encephalomyelitisJohn W Chen
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 5404 Building 149, 13th Street, Charlestown, MA 02129, USA
Brain 131:1123-33. 2008..This approach could be used in longitudinal studies to identify active demyelinating plaques as well as to more accurately track disease course following treatment in clinical trials...
Vasculitis: molecular imaging by targeting the inflammatory enzyme myeloperoxidaseHenry S Su
Center for Molecular Imaging Research, Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
Radiology 262:181-90. 2012..To determine if a molecular imaging approach targeting the highly oxidative enzyme myeloperoxidase (MPO) can help noninvasively identify and confirm sites of vascular wall inflammation in a murine model of vasculitis...- Demyelinating diseases: myeloperoxidase as an imaging biomarker and therapeutic targetReza Forghani
Center for Systems Biology, Harvard Medical School, Richard B Simches Research Center, Boston, MA 02114, USA
Radiology 263:451-60. 2012.... - Bevacizumab with angiostatin-armed oHSV increases antiangiogenesis and decreases bevacizumab-induced invasion in U87 gliomaWei Zhang
Brain Tumor Research Center, Department of Neurosurgery, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
Mol Ther 20:37-45. 2012.... - Distinguishing inflammation from tumor and peritumoral edema by myeloperoxidase magnetic resonance imagingAnne Kleijn
Brain Tumor Research Center, Massachusetts General Hospital, Boston, Massachusetts, USA
Clin Cancer Res 17:4484-93. 2011..The imaging results were compared with histopathologic and molecular analyses of the tumors for macrophage/microglia infiltration, virus persistence, and MPO levels... - Tracking the inflammatory response in stroke in vivo by sensing the enzyme myeloperoxidaseMichael O Breckwoldt
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, CNY 149, 13th Street, Charlestown, MA 02129, USA
Proc Natl Acad Sci U S A 105:18584-9. 2008..Our findings could allow efficient noninvasive serial screening of therapies targeting inflammation and the use of MPO imaging as an imaging biomarker to risk-stratify patients... - Human myeloperoxidase: a potential target for molecular MR imaging in atherosclerosisJohn W Chen
Center for Molecular Imagina Research, Massachusetts General Hospital, Charlestown 02129, USA
Magn Reson Med 52:1021-8. 2004..As a result, we were able to demonstrate MPO activity in enzyme solutions and in a model tissue-like system. These studies suggest that activatable paramagnetic MR imaging agents can be used to directly image MPO activity... - Single reporter for targeted multimodal in vivo imagingJohanna M Niers
Experimental Therapeutics and Molecular Imaging Laboratory, Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Boston, United States
J Am Chem Soc 134:5149-56. 2012.... - Ligation of the jugular veins does not result in brain inflammation or demyelination in miceWendy Atkinson
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Richard B Simches Research Center, Boston, Massachusetts, United States of America
PLoS ONE 7:e33671. 2012..Therefore, our murine model does not support CCSVI as a cause of demyelinating diseases such as multiple sclerosis... - Myeloperoxidase-rich Ly-6C+ myeloid cells infiltrate allografts and contribute to an imaging signature of organ rejection in miceFilip K Swirski
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
J Clin Invest 120:2627-34. 2010..Thus, MPO imaging represents a potential alternative to the current invasive clinical standard by which transplants are monitored... - Activatable magnetic resonance imaging agents for myeloperoxidase sensing: mechanism of activation, stability, and toxicityElisenda Rodriguez
Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
J Am Chem Soc 132:168-77. 2010..Gd kinetic lability and cytotoxicity studies further confirm stability of the Gd ion and low toxicity for the 5HT-based agents, suggesting that these agents are suitable for translational in vivo studies... - Combined magnetic resonance and fluorescence imaging of the living mouse brain reveals glioma response to chemotherapyCorey M McCann
Center for Molecular Imaging Research, Massachusetts General Hospital, 185 Cambridge Street, Boston, Massachusetts 02114, USA
Neuroimage 45:360-9. 2009..Our results reveal that combined FMT-MR imaging of fluorescent molecular probes could be valuable for brain tumor drug development and other neurological and somatic imaging applications... - Myeloperoxidase activity imaging using (67)Ga labeled substrateManel Querol Sans
Department of Radiology, University of Massachusetts Medical School, 55 Lake Ave North, Worcester, MA 01655, USA
Mol Imaging Biol 7:403-10. 2005..CONCLUSION: (67)Ga-I can be used to image MPO activity in a model system. The accumulation mechanism is based on a differential pharmacokinetics because of the size increase resulting from (67)Ga-I interaction with the target enzyme... - Angiotensin II Drives the Production of Tumor-Promoting MacrophagesVirna Cortez-Retamozo
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Immunity 38:296-308. 2013..These findings indicate that AngII acts upstream of a potent macrophage amplification program and that tumors can remotely exploit the hormone's pathway to stimulate cancer-promoting immunity... - Selective factor XIIa inhibition attenuates silent brain ischemia: application of molecular imaging targeting coagulation pathwayJohn W Chen
Center for Systems Biology, Boston, Massachusetts 02114, USA
JACC Cardiovasc Imaging 5:1127-38. 2012..The purpose of this study was use molecular imaging targeting coagulation pathway and inflammation to better understand the pathophysiology of silent brain ischemia (SBI) and monitor the effects of factor XIIa inhibition... - Activatable magnetic resonance imaging agent reports myeloperoxidase activity in healing infarcts and noninvasively detects the antiinflammatory effects of atorvastatin on ischemia-reperfusion injuryMatthias Nahrendorf
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Circulation 117:1153-60. 2008..We hypothesized that MPO imaging could be used to measure inflammatory responses after myocardial ischemia locally and noninvasively in a murine model... - Enhanced in vivo imaging of metabolically biotinylated cell surface reportersJohanna M Niers
Neuroscience Center, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
Anal Chem 83:994-9. 2011..These findings have practical applications in designing more efficient targeting and imaging strategies... - DTPA-bisamide-based MR sensor agents for peroxidase imagingManuel Querol
Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
Org Lett 7:1719-22. 2005..Both derivatives bear tyramido or 5-hydroxytryptamido groups that could be oligomerized in situ in the presence of peroxidase/H(2)O(2) pair resulting in a net increase in longitudinal (R1) relaxivity... - Origins of tumor-associated macrophages and neutrophilsVirna Cortez-Retamozo
Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 109:2491-6. 2012..The present study sheds light on the origins of TAMs and TANs, and positions the spleen as an important extramedullary site, which can continuously supply growing tumors with these cells... - Metabolic biotinylation of cell surface receptors for in vivo imagingBakhos A Tannous
Center for Molecular Imaging Research, Massachusetts General Hospital-East, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
Nat Methods 3:391-6. 2006..This BAP-TM allows noninvasive real-time imaging of any cell type transduced to express this reporter protein in culture or in vivo... - Oncogenic EGFR signaling cooperates with loss of tumor suppressor gene functions in gliomagenesisHaihao Zhu
Molecular Oncology Research Institute, Department of Neurosurgery, Tufts University School of Medicine, Tufts Medical Center, 800 Washington Street, Boston, MA 02111, USA
Proc Natl Acad Sci U S A 106:2712-6. 2009..Our findings provide critical insights into the role of mutant EGFR signaling function in GBM tumor biology and set the stage for testing of targeted therapeutic agents in the preclinical models described herein... - A paramagnetic contrast agent with myeloperoxidase-sensing propertiesManel Querol
University of Massachusetts Medical School, 55 Lake Avenue, Worcester, Massachusetts 01655, USA
Org Biomol Chem 4:1887-95. 2006..We expected that upon activation by MPO/H2O2 L1-Gd3+ would not only oligomerise but also bind to other macromolecules present in the media and that the overall process will give rise to a net T1-weighted MRI signal increase...
Research Grants
- Myeloperoxidase, imaging biomarker and treatment target for multiple sclerosisJohn W Chen; Fiscal Year: 2010....
- Molecular imaging of vascular pathologyJohn Chen; Fiscal Year: 2007....
- Imaging and Treating Inflammation in Stroke by Targeting MyeloperoxidaseJohn W Chen; Fiscal Year: 2010....