S Buratowski

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Bur1 kinase is required for efficient transcription elongation by RNA polymerase II
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 23:7005-18. 2003
  2. ncbi request reprint Distinct pathways for snoRNA and mRNA termination
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 24:723-34. 2006
  3. pmc The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:795-804. 2008
  4. ncbi request reprint Snapshots of RNA polymerase II transcription initiation
    S Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Curr Opin Cell Biol 12:320-5. 2000
  5. ncbi request reprint Connections between mRNA 3' end processing and transcription termination
    Stephen Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Opin Cell Biol 17:257-61. 2005
  6. pmc The role of cotranscriptional histone methylations
    S Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Symp Quant Biol 75:95-102. 2010
  7. pmc Progression through the RNA polymerase II CTD cycle
    Stephen Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 36:541-6. 2009
  8. pmc Bromodomain factor 1 corresponds to a missing piece of yeast TFIID
    O Matangkasombut
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 14:951-62. 2000
  9. pmc The essential interaction between yeast mRNA capping enzyme subunits is not required for triphosphatase function in vivo
    Y Takase
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 20:9307-16. 2000
  10. pmc Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain
    E J Cho
    Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston, Massachusetts 02115, USA
    Genes Dev 15:3319-29. 2001

Research Grants

  1. RNA POLYMERASE II TRANSCRIPTION INITIATION COMPLEX
    Stephen Buratowski; Fiscal Year: 1999
  2. MRNA CAPPING ENZYME
    Stephen Buratowski; Fiscal Year: 2002
  3. MRNA CAPPING ENZYME
    Stephen Buratowski; Fiscal Year: 2006
  4. RNA POLYMERASE II TRANSCRIPTION INITIATION COMPLEX
    Stephen Buratowski; Fiscal Year: 2007
  5. The RNA polymerase II transcription complex
    Stephen Buratowski; Fiscal Year: 2010
  6. RNA POLYMERASE II TRANSCRIPTION INITIATION COMPLEX
    Stephen Buratowski; Fiscal Year: 2003
  7. MRNA CAPPING ENZYME
    Stephen Buratowski; Fiscal Year: 2010
  8. MRNA CAPPING ENZYME
    Stephen Buratowski; Fiscal Year: 2007
  9. The RNA polymerase II transcription complex
    Stephen Buratowski; Fiscal Year: 2009

Collaborators

Detail Information

Publications65

  1. pmc Bur1 kinase is required for efficient transcription elongation by RNA polymerase II
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 23:7005-18. 2003
    ..These results show that Bur1 functions in transcription elongation but may phosphorylate a substrate other than the CTD...
  2. ncbi request reprint Distinct pathways for snoRNA and mRNA termination
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 24:723-34. 2006
    ..These results suggest that in yeast the transcribing RNA polymerase II can choose between two distinct termination mechanisms but keeps both options available during elongation...
  3. pmc The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nat Struct Mol Biol 15:795-804. 2008
    ..Nrd1 recruitment to genes involves a combination of interactions with CTD and Nab3...
  4. ncbi request reprint Snapshots of RNA polymerase II transcription initiation
    S Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Curr Opin Cell Biol 12:320-5. 2000
    ..A high resolution picture of the transcription complex is likely to be available within the foreseeable future. The challenge is to determine the roles of individual proteins within this surprisingly large molecular machine...
  5. ncbi request reprint Connections between mRNA 3' end processing and transcription termination
    Stephen Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Curr Opin Cell Biol 17:257-61. 2005
    ..Some recent reports are shedding light on these connections...
  6. pmc The role of cotranscriptional histone methylations
    S Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cold Spring Harb Symp Quant Biol 75:95-102. 2010
    ....
  7. pmc Progression through the RNA polymerase II CTD cycle
    Stephen Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 36:541-6. 2009
    ..Recent papers help to explain how the changes in CTD phosphorylation pattern are linked to the progression from initiation through elongation to termination...
  8. pmc Bromodomain factor 1 corresponds to a missing piece of yeast TFIID
    O Matangkasombut
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 14:951-62. 2000
    ..The structural and functional similarities suggest that Bdf1 corresponds to the carboxy-terminal region of higher eukaryotic TAF(II)250 and that the interaction between TFIID and Bdf1 is important for proper gene expression...
  9. pmc The essential interaction between yeast mRNA capping enzyme subunits is not required for triphosphatase function in vivo
    Y Takase
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 20:9307-16. 2000
    ..These results strongly suggest that the primary physiological role of the Ceg1-Cet1 interaction is to allosterically activate Ceg1, rather than to recruit Cet1 to the Pol II complex...
  10. pmc Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain
    E J Cho
    Harvard Medical School, Department of Biological Chemistry and Molecular Pharmacology, Boston, Massachusetts 02115, USA
    Genes Dev 15:3319-29. 2001
    ..Our results suggest that this regulation may occur by modulating levels of Ser 2 phosphorylation, which in turn, may regulate the association of elongation factors with the polymerase...
  11. pmc Yeast homologues of higher eukaryotic TFIID subunits
    Z Moqtaderi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 93:14654-8. 1996
    ..Yeast TAF61 is significantly larger than its higher eukaryotic homologues, and deletion analysis demonstrates that the evolutionarily conserved, histone-like domain is sufficient and necessary to support viability...
  12. pmc The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein
    H Morehouse
    Department of Biological Chemistry, Harvard Medical School, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 96:12542-7. 1999
    ..Kap114 binds TBP in vitro, and binding is disrupted in the presence of GTPgammaS. Therefore, Kap114 is an importer of TBP into the nucleus, but alternative import pathways must also exist...
  13. pmc Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain
    E J Cho
    Harvard Medical School, Boston, Massachusetts 02115 USA
    Genes Dev 12:3482-7. 1998
    ..Therefore, the yeast mRNA guanylyltransferase is regulated by allosteric interactions with both the triphosphatase and CTD...
  14. pmc Conditional mutants of the yeast mRNA capping enzyme show that the cap enhances, but is not required for, mRNA splicing
    L D Fresco
    Harvard Medical School, Boston, Massachusetts 02115, USA
    RNA 2:584-96. 1996
    ..In addition, steady-state levels of several mRNAs were decreased, perhaps due to increased degradation of uncapped mRNAs. In contrast to splicing, mRNA polyadenylation and transport to the cytoplasm were unaffected...
  15. ncbi request reprint Histone-like TAFs are essential for transcription in vivo
    B Michel
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 2:663-73. 1998
    ..Therefore, in contrast to previous studies in yeast that found only limited roles for TAFs in transcription, we find that the histone-like TAFs are generally required for in vivo transcription...
  16. pmc mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain
    E J Cho
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 11:3319-26. 1997
    ..Our results provide in vitro and in vivo evidence that capping enzyme is recruited to the transcription complex via phosphorylation of the RNA polymerase CTD...
  17. pmc TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo
    P B Komarnitsky
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 13:2484-9. 1999
    ..Here we show that mutations in the yeast TFIID-specific protein Taf40 lead to a general cessation of transcription, even in the presence of excess TBP, suggesting that the TFIID complex is required at most promoters in vivo...
  18. pmc A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme
    C R Rodriguez
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Nucleic Acids Res 27:2181-8. 1999
    ..Ctl1 is the second member of the yeast RNA triphosphatase family, but is probably involved in an RNA processing event other than mRNA capping...
  19. pmc Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II
    C R Rodriguez
    Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 20:104-12. 2000
    ..Pta1 in yeast extracts binds specifically to the phosphorylated CTD, suggesting that this interaction may mediate coupling of polyadenylation and transcription...
  20. ncbi request reprint Evidence that transcription factor IIB is required for a post-assembly step in transcription initiation
    E J Cho
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 274:25807-13. 1999
    ..This step may be related to the yeast-specific spacing between TATA elements and start sites since mutations of the corresponding glutamate in mammalian TFIIB do not produce a similar effect...
  21. ncbi request reprint Phosphorylation of serine 2 within the RNA polymerase II C-terminal domain couples transcription and 3' end processing
    Seong Hoon Ahn
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 13:67-76. 2004
    ..Therefore, Serine 2 phosphorylation by Ctk1 recruits factors for cotranscriptional 3' end processing in vivo...
  22. ncbi request reprint The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 432:517-22. 2004
    ..These findings support a model in which poly(A) site cleavage and subsequent degradation of the 3'-downstream RNA by Rat1 trigger transcription termination...
  23. pmc Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation
    JESSICA L DERMODY
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    PLoS ONE 3:e3273. 2008
    ..This work defines a novel role for Npl3 in elongation and its regulation by phosphorylation...
  24. ncbi request reprint Mobility shift DNA-binding assay using gel electrophoresis
    S Buratowski
    Harvard Medical School, Boston, Massachusetts, USA
    Curr Protoc Mol Biol . 2001
    ..Three additional protocols describe a competition assay using unlabeled competitor DNA, an antibody supershift assay, and multicomponent gel shift assays...
  25. pmc Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 284:26421-6. 2009
    ..The basal factor TFIIH can phosphorylate Ser-7 in vitro and is necessary for Ser-7(P) in vivo. Interestingly, deletion of the CTD Ser-5(P) phosphatase Rtr1 leads to an increase in Ser-5(P) but not Ser-7(P)...
  26. pmc Transitions in RNA polymerase II elongation complexes at the 3' ends of genes
    Minkyu Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    EMBO J 23:354-64. 2004
    ..Therefore, transcription termination and polyadenylation involve transitions at the 3' end of genes that may include an exchange of elongation and polyadenylation/termination factors...
  27. pmc Npl3 is an antagonist of mRNA 3' end formation by RNA polymerase II
    Miriam E Bucheli
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 24:2150-60. 2005
    ..These results suggest that elongation rate and mRNA packaging can influence polyadenylation and termination...
  28. ncbi request reprint Two Saccharomyces cerevisiae JmjC domain proteins demethylate histone H3 Lys36 in transcribed regions to promote elongation
    Taesoo Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 282:20827-35. 2007
    ..Taken together, these findings indicate that a general function of histone demethylases for H3 Lys(36) is to promote transcription elongation by antagonizing repressive Lys(36) methylation by Set2...
  29. pmc Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions
    N H Kuldell
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 17:5288-98. 1997
    ..Finally, we have demonstrated the biological importance of TFIIE in pol II-mediated transcription by depleting the Tfa1 protein from the cells and observing a concomitant decrease in total poly(A)+ mRNA...
  30. doi request reprint Transcription termination and RNA degradation contribute to silencing of RNA polymerase II transcription within heterochromatin
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 29:313-23. 2008
    ..The Nrd1/Sen1/exosome pathway also contributes to silencing at telomeric loci. These results suggest that silencing of heterochromatic genes in Saccharomyces cerevisiae occurs at both transcriptional and posttranscriptional levels...
  31. pmc Reconstitution of heterochromatin-dependent transcriptional gene silencing
    Aaron Johnson
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell 35:769-81. 2009
    ..These results define a minimal set of components that mediate heterochromatic gene silencing and demonstrate distinct contributions for histone deacetylation and nucleosome binding in the silencing mechanism...
  32. pmc Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 22:1288-97. 2002
    ..Surprisingly, these phosphorylation site mutants appear to destabilize the association of the cyclin subunit within the context of TFIIH but not within the trimer complex...
  33. pmc RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast
    Tae Soo Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard University, 240 Longwood Avenue, Boston, MA 02115, USA
    Genome Biol 11:R75. 2010
    ..However, changes in mRNA abundance reflect the combined effect of changes in RNA production, processing, and degradation, and thus, mRNA levels provide an occluded view of transcriptional regulation...
  34. ncbi request reprint An RNA 5'-triphosphatase related to the protein tyrosine phosphatases
    T Takagi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 89:867-73. 1997
    ..These results broaden the superfamily of PTP-like phosphatases to include enzymes with RNA substrates...
  35. ncbi request reprint Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts
    Lidia Vasiljeva
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Cell 21:239-48. 2006
    ..Since Nrd1 is known to bind RNA polymerase II and be important for sn/snoRNA 3' end processing, Nrd1 may link transcription and RNA 3' end formation with surveillance by the exosome...
  36. pmc Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment
    JESSICA L DERMODY
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 285:33671-9. 2010
    ..Together, these results suggest that association of Paf1C with RNA stabilizes its localization at actively transcribed regions where it influences chromatin structure...
  37. pmc Polyadenylation site choice in yeast is affected by competition between Npl3 and polyadenylation factor CFI
    Miriam E Bucheli
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    RNA 13:1756-64. 2007
    ..Our results support a model in which balanced competition of Npl3 with mRNA processing factors may promote recognition of proper polyadenylation sites while suppressing cryptic sites...
  38. pmc The Rpb4 subunit of RNA polymerase II contributes to cotranscriptional recruitment of 3' processing factors
    Vanessa M Runner
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell Biol 28:1883-91. 2008
    ..Furthermore, loss of Rpb4 results in altered polyadenylation site usage at the RNA14 gene. Together, these results indicate that Rpb4 contributes to proper cotranscriptional 3'-end processing in vivo...
  39. pmc The basal initiation machinery: beyond the general transcription factors
    Timothy W Sikorski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, United States
    Curr Opin Cell Biol 21:344-51. 2009
    ..Recent studies are expanding our understanding of the roles of these factors, demonstrating that their functions are both broader and more context dependent than previously realized...
  40. ncbi request reprint A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nature 439:497-501. 2006
    ..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint...
  41. ncbi request reprint Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in Saccharomyces cerevisiae
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 257:1-16. 2004
    ..Although this technique was originally designed to assay DNA binding proteins, it can also be used to monitor mRNA processing factors associated with transcription complexes...
  42. pmc Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2
    Chika Sawa
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Biol 24:4734-42. 2004
    ..A similar mechanism may be responsible for phosphorylation of the C-terminal region of mammalian TAF1. These findings suggest that CK2 phosphorylation of Bdf1 may regulate RNA polymerase II transcription and/or chromatin structure...
  43. ncbi request reprint Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function
    Roy Auty
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    J Biol Chem 279:49973-81. 2004
    ..TFIID supports basal transcription and activated transcription, both of which are enhanced by TFIIA...
  44. ncbi request reprint Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell 123:593-605. 2005
    ..This pathway apparently acts to negatively regulate transcription because deleting the genes for Set2 or Rpd3C(S) bypasses the requirement for the positive elongation factor Bur1/Bur2...
  45. ncbi request reprint Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation
    Oranart Matangkasombut
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Mol Cell 11:353-63. 2003
    ..Bromodomain point mutations that block Bdf1 binding to histones disrupt transcription and reduce Bdf1 association with chromatin in vivo. Therefore, bromodomains with different specificity generate further complexity of the histone code...
  46. ncbi request reprint The Caenorhabditis elegans mRNA 5'-capping enzyme. In vitro and in vivo characterization
    Toshimitsu Takagi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:14174-84. 2003
    ..elegans embryos shows that lack of CEL-1 causes development to arrest with a phenotype similar to that seen when RNA polymerase II elongation activity is disrupted. Therefore, capping is essential for gene expression in metazoans...
  47. pmc Divergent subunit interactions among fungal mRNA 5'-capping machineries
    Toshimitsu Takagi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 021151, USA
    Eukaryot Cell 1:448-57. 2002
    ..cerevisiae GTase. The C. albicans capping enzyme subunits do interact with each other. However, this interaction is not essential in vivo. Our results reveal an unexpected diversity among the fungal capping machineries...
  48. pmc Dimethylation of H3K4 by Set1 recruits the Set3 histone deacetylase complex to 5' transcribed regions
    Taesoo Kim
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA
    Cell 137:259-72. 2009
    ....
  49. pmc H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway
    Nihal Terzi
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA
    Mol Cell Biol 31:3569-83. 2011
    ..We speculate that Set1 promotes proper early termination by the Nrd1-Nab3-Sen1 complex by affecting the kinetics of Pol II transcription in early elongation...
  50. ncbi request reprint Structure and function of the TFIID complex
    Oranart Matangkasombut
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02215, USA
    Adv Protein Chem 67:67-92. 2004
  51. pmc Interdependent interactions between TFIIB, TATA binding protein, and DNA
    Robin M Buratowski
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell Biol 22:8735-43. 2002
    ..The TBPs with increased affinity could not suppress TFIIB(G204D), leading us to propose a two-step model for the interaction between TFIIB and the TBP-DNA complex...
  52. pmc The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4
    Michael Christopher Keogh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Genes Dev 20:660-5. 2006
    ..Function-specific modifications may help explain how the same component of chromatin can function in diverse pathways...
  53. ncbi request reprint gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair
    Dipanjan Chowdhury
    CBR Institute for Biomedical Research and The Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 20:801-9. 2005
    ..The effect of PP2A on gamma-H2AX levels is independent of ATM, ATR, or DNA-PK activity...
  54. ncbi request reprint Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends
    Eduard Nedea
    Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5G 1L6, Canada
    J Biol Chem 278:33000-10. 2003
    ..Ref2 and Pta1 similarly affect at least one snoRNA transcript...
  55. pmc RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach
    Nevan J Krogan
    Banting and Best Department of Medical Research Department of Molecular and Medical Genetics, University of Toronto TYPO, Toronto Yeast Proteomics Organization, Toronto, Ontario, Canada
    Mol Cell Biol 22:6979-92. 2002
    ..Spt5, Spt6, and Iws1 are phosphorylated on consensus CKII sites in vivo, conceivably by the Chd1/CKII associated with Spt16/Pob3. All the elongation factors but Elongator copurified with RNAPII...
  56. pmc Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II
    Nevan J Krogan
    Banting and Best Department of Medical Research, Toronto Yeast Proteomics Organization, University of Toronto, Toronto, Ontario, Canada M5G 1L6
    Mol Cell Biol 23:4207-18. 2003
    ..SET2 also interacts genetically with components of the Set1 and Set3 complexes, suggesting that Set1, Set2, and Set3 similarly affect transcription by RNAPII...
  57. ncbi request reprint The CTD code
    Stephen Buratowski
    Nat Struct Biol 10:679-80. 2003
  58. ncbi request reprint Gene regulation: expression and silencing coupled
    Stephen Buratowski
    Nature 435:1174-5. 2005
  59. ncbi request reprint Dynamics of replication-independent histone turnover in budding yeast
    Michael F Dion
    Faculty of Arts and Sciences, Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA
    Science 315:1405-8. 2007
    ..In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states...
  60. pmc Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4
    Nevan J Krogan
    Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario, Canada M5G 1L6
    Proc Natl Acad Sci U S A 101:13513-8. 2004
    ..Because SWR-C-dependent recruitment of Htz1 occurs in both transcribed and centromeric regions, a NuA4/SWR-C/Htz1 pathway may regulate both transcription and centromere function in S. cerevisiae...
  61. ncbi request reprint Proteasome involvement in the repair of DNA double-strand breaks
    Nevan J Krogan
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario M5G 1L6, Canada
    Mol Cell 16:1027-34. 2004
    ....
  62. ncbi request reprint A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1
    Nevan J Krogan
    Banting and Best Department of Medical Research, University of Toronto, 112 College Street, Toronto, Ontario, Canada M5G 1L6
    Mol Cell 12:1565-76. 2003
    ..We show that recruitment of Htz1 to chromatin requires the SWR-C. Moreover, like Htz1 and Bdf1, the SWR-C promotes gene expression near silent heterochromatin...
  63. pmc Genes with internal repeats require the THO complex for transcription
    Vladimir Voynov
    Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 103:14423-8. 2006
    ..The Tho- defect in transcription is suppressed by overexpression of topoisomerase I, suggesting that the THO complex functions to rectify aberrant structures that arise during transcription...
  64. pmc Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals
    Pritilekha Deka
    Department of Chemistry, University of Washington, Seattle, WA 98195, USA
    J Mol Biol 375:136-50. 2008
    ....
  65. pmc Single-nucleosome mapping of histone modifications in S. cerevisiae
    Chih Long Liu
    Bauer Center for Genomics Research, Harvard University, Cambridge, Massachusetts, USA
    PLoS Biol 3:e328. 2005
    ..g., promoter nucleosomes) from those at another location (e.g., over the 3' ends of coding regions). These results are consistent with the idea of a simple, redundant histone code, in which multiple modifications share the same role...

Research Grants29

  1. RNA POLYMERASE II TRANSCRIPTION INITIATION COMPLEX
    Stephen Buratowski; Fiscal Year: 1999
    ..The proteins identified as suppressors (whether known factors or new proteins) will be characterized as to their interactions with the transcription machinery. ..
  2. MRNA CAPPING ENZYME
    Stephen Buratowski; Fiscal Year: 2002
    ..Although many aspects are conserved over evolution, there appear to major differences between yeast and higher eukaryotic capping enzymes that could be exploited for antifungal drug targeting. ..
  3. MRNA CAPPING ENZYME
    Stephen Buratowski; Fiscal Year: 2006
    ..A clear understanding of the fundamental mechanisms of gene expression will provide the groundwork for future therapies, including gene replacement therapies and direct modulation of cellular and viral gene expression. ..
  4. RNA POLYMERASE II TRANSCRIPTION INITIATION COMPLEX
    Stephen Buratowski; Fiscal Year: 2007
    ..Since improper gene expression is often at the root of cancer and developmental diseases, a clear understanding of the gene expression machinery will be required to develop effective therapeutics. ..
  5. The RNA polymerase II transcription complex
    Stephen Buratowski; Fiscal Year: 2010
    ..This fundamental knowledge is essential for understanding how mutations in transcription factors and histone modifying enzymes lead to diseases such as cancer and developmental defects. ..
  6. RNA POLYMERASE II TRANSCRIPTION INITIATION COMPLEX
    Stephen Buratowski; Fiscal Year: 2003
    ..The proteins identified as suppressors (whether known factors or new proteins) will be characterized as to their interactions with the transcription machinery. ..
  7. MRNA CAPPING ENZYME
    Stephen Buratowski; Fiscal Year: 2010
    ..A clear understanding of the fundamental mechanisms of gene expression will provide the groundwork for future therapies, including gene replacement therapies and direct modulation of cellular and viral gene expression. ..
  8. MRNA CAPPING ENZYME
    Stephen Buratowski; Fiscal Year: 2007
    ..A clear understanding of the fundamental mechanisms of gene expression will provide the groundwork for future therapies, including gene replacement therapies and direct modulation of cellular and viral gene expression. ..
  9. The RNA polymerase II transcription complex
    Stephen Buratowski; Fiscal Year: 2009
    ..This fundamental knowledge is essential for understanding how mutations in transcription factors and histone modifying enzymes lead to diseases such as cancer and developmental defects. ..