Dennis Brown

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc Basolateral targeting and microtubule-dependent transcytosis of the aquaporin-2 water channel
    Naofumi Yui
    Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 304:C38-48. 2013
  2. pmc New insights into the dynamic regulation of water and acid-base balance by renal epithelial cells
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Simches Research Center, Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 302:C1421-33. 2012
  3. pmc Molecular mechanisms of acid-base sensing by the kidney
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology, Boston, MA, USA
    J Am Soc Nephrol 23:774-80. 2012
  4. pmc Regulation of the V-ATPase in kidney epithelial cells: dual role in acid-base homeostasis and vesicle trafficking
    Dennis Brown
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, MA 02114, USA
    J Exp Biol 212:1762-72. 2009
  5. pmc Sensing, signaling and sorting events in kidney epithelial cell physiology
    Dennis Brown
    Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Traffic 10:275-84. 2009
  6. ncbi request reprint Compensatory membrane expression of the V-ATPase B2 subunit isoform in renal medullary intercalated cells of B1-deficient mice
    Teodor G Paunescu
    Div of Nephrology, Massachusetts General Hospital, 185 Cambridge St, CPZN 8150, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 293:F1915-26. 2007
  7. ncbi request reprint Relocalization of the V-ATPase B2 subunit to the apical membrane of epididymal clear cells of mice deficient in the B1 subunit
    Nicolas Da Silva
    Massachusetts General Hospital, Harvard Medical School, Program in Membrane Biology, Nephrology Div, 185 Cambridge St, CPZN 8150, Boston, MA 02114 2790, USA
    Am J Physiol Cell Physiol 293:C199-210. 2007
  8. ncbi request reprint Association of soluble adenylyl cyclase with the V-ATPase in renal epithelial cells
    Teodor G Paunescu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 294:F130-8. 2008
  9. pmc cAMP stimulates apical V-ATPase accumulation, microvillar elongation, and proton extrusion in kidney collecting duct A-intercalated cells
    Teodor G Paunescu
    MGH Center for Systems Biology, Program in Membrane Biology, and Division of Nephrology, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 298:F643-54. 2010
  10. pmc Acute hypertonicity alters aquaporin-2 trafficking and induces a MAPK-dependent accumulation at the plasma membrane of renal epithelial cells
    Udo Hasler
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts 02114 2790, USA
    J Biol Chem 283:26643-61. 2008

Collaborators

Detail Information

Publications76

  1. pmc Basolateral targeting and microtubule-dependent transcytosis of the aquaporin-2 water channel
    Naofumi Yui
    Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 304:C38-48. 2013
    ..Furthermore, our data indicate that protein polarity data obtained from biotinylation assays, which require cells to be cooled to 4°C during the labeling procedure, should be interpreted with caution...
  2. pmc New insights into the dynamic regulation of water and acid-base balance by renal epithelial cells
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Simches Research Center, Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 302:C1421-33. 2012
    ..This review summarizes recent advances in our understanding of these fundamental processes...
  3. pmc Molecular mechanisms of acid-base sensing by the kidney
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology, Boston, MA, USA
    J Am Soc Nephrol 23:774-80. 2012
    ..This review collates current knowledge on renal acid-base sensing in the context of a whole organ sensing and response process...
  4. pmc Regulation of the V-ATPase in kidney epithelial cells: dual role in acid-base homeostasis and vesicle trafficking
    Dennis Brown
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, MA 02114, USA
    J Exp Biol 212:1762-72. 2009
    ..Recent information about these pH-sensing mechanisms, which include the role of the V-ATPase itself as a pH sensor and the soluble adenylyl cyclase as a bicarbonate sensor, will be addressed in this review...
  5. pmc Sensing, signaling and sorting events in kidney epithelial cell physiology
    Dennis Brown
    Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Traffic 10:275-84. 2009
    ....
  6. ncbi request reprint Compensatory membrane expression of the V-ATPase B2 subunit isoform in renal medullary intercalated cells of B1-deficient mice
    Teodor G Paunescu
    Div of Nephrology, Massachusetts General Hospital, 185 Cambridge St, CPZN 8150, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 293:F1915-26. 2007
    ..However, our results show no increase in cell surface V-ATPase activity in response to metabolic acidosis in ICs from these animals, consistent with their inability to appropriately acidify their urine under these conditions...
  7. ncbi request reprint Relocalization of the V-ATPase B2 subunit to the apical membrane of epididymal clear cells of mice deficient in the B1 subunit
    Nicolas Da Silva
    Massachusetts General Hospital, Harvard Medical School, Program in Membrane Biology, Nephrology Div, 185 Cambridge St, CPZN 8150, Boston, MA 02114 2790, USA
    Am J Physiol Cell Physiol 293:C199-210. 2007
    ..This relocation compensates for the absence of functional B1 and maintains the luminal pH in an acidic range that is compatible with fertility...
  8. ncbi request reprint Association of soluble adenylyl cyclase with the V-ATPase in renal epithelial cells
    Teodor G Paunescu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 294:F130-8. 2008
    ..This suggests that these enzymes have a close association and could be part of a protein complex that is involved in regulating renal distal proton secretion...
  9. pmc cAMP stimulates apical V-ATPase accumulation, microvillar elongation, and proton extrusion in kidney collecting duct A-intercalated cells
    Teodor G Paunescu
    MGH Center for Systems Biology, Program in Membrane Biology, and Division of Nephrology, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 298:F643-54. 2010
    ....
  10. pmc Acute hypertonicity alters aquaporin-2 trafficking and induces a MAPK-dependent accumulation at the plasma membrane of renal epithelial cells
    Udo Hasler
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts 02114 2790, USA
    J Biol Chem 283:26643-61. 2008
    ..This may have important implications on adaptational processes governing transcellular water flux and/or cell survival under extreme conditions of hypertonicity...
  11. pmc Calcitonin has a vasopressin-like effect on aquaporin-2 trafficking and urinary concentration
    Richard Bouley
    Massachusetts General Hospital, Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Simches Research Center, Room 8202, 185 Cambridge Street, Boston, MA 02114, USA
    J Am Soc Nephrol 22:59-72. 2011
    ..This suggests that calcitonin has a potential therapeutic use in nephrogenic diabetes insipidus...
  12. pmc Role of purinergic signaling pathways in V-ATPase recruitment to apical membrane of acidifying epididymal clear cells
    Clémence Belleannee
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Am J Physiol Cell Physiol 298:C817-30. 2010
    ....
  13. ncbi request reprint Aquaporin 2 (AQP2) and vasopressin type 2 receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in 'endocytosis-resistant' membrane domains after vasopressin treatment
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Biol Cell 98:215-32. 2006
    ..In the present study, we further investigated AQP2 accumulation at the cell surface, and compared it with V2R (VP type 2 receptor) trafficking using cells that express epitope-tagged AQP2 and V2R...
  14. pmc Heterologous downregulation of vasopressin type 2 receptor is induced by transferrin
    Richard Bouley
    1Nephrology Division, MGH Center for Systems Biology, Program in Membrane Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 304:F553-64. 2013
    ..It also provides new insights into urine-concentrating defects observed in rat models of hemochromatosis...
  15. ncbi request reprint Modulation of the actin cytoskeleton via gelsolin regulates vacuolar H+-ATPase recycling
    Valerie Beaulieu
    Program in Membrane Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    J Biol Chem 280:8452-63. 2005
    ..Therefore, modulation of the actin cortex by this severing and capping protein may represent a common mechanism by which these cells regulate their rate of proton secretion...
  16. ncbi request reprint V-ATPase interacts with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway
    Andres Hurtado-Lorenzo
    Program in Membrane Biology and Nephrology Division, Richard Simches Research Center, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, MA 02114, USA
    Nat Cell Biol 8:124-36. 2006
    ....
  17. pmc Regulation of V-ATPase recycling via a RhoA- and ROCKII-dependent pathway in epididymal clear cells
    Winnie Waichi Shum
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Cell Physiol 301:C31-43. 2011
    ..We propose that inhibition of RhoA-ROCKII might be part of the intracellular signaling cascade that is triggered upon agonist-induced apical membrane V-ATPase accumulation...
  18. pmc Differential, phosphorylation dependent trafficking of AQP2 in LLC-PK1 cells
    William L Rice
    Program in Membrane Biology, Division of Nephrology, Center for Systems Biology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 7:e32843. 2012
    ..Overall, our data provide evidence supporting the role of S256 and S269 in the maintenance of AQP2 at the cell surface and reveal the dynamics of internalization and recycling of differentially phosphorylated AQP2 in cell culture...
  19. ncbi request reprint Role of NHERF1, cystic fibrosis transmembrane conductance regulator, and cAMP in the regulation of aquaporin 9
    Christine Pietrement
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Simches Research Center, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA
    J Biol Chem 283:2986-96. 2008
    ..We propose that CFTR is an important regulator of AQP9 and that the interaction between AQP9, NHERF1, and CFTR may facilitate the activation of AQP9 by cAMP...
  20. ncbi request reprint Membrane organization and function of M1 and M23 isoforms of aquaporin-4 in epithelial cells
    Claudia Silberstein
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 287:F501-11. 2004
    ....
  21. pmc Simvastatin enhances aquaporin-2 surface expression and urinary concentration in vasopressin-deficient Brattleboro rats through modulation of Rho GTPase
    Wei Li
    Center for Systems Biology, Program in Membrane Biology, Division of Nephrology, Dept of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 301:F309-18. 2011
    ..Our study provides an alternative mechanism to regulate AQP2 trafficking, bypassing the VP-vasopressin receptor signaling pathway...
  22. ncbi request reprint Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking
    Hua A J Lu
    Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 282:28721-32. 2007
    ..To summarize, we have identified the 70-kDa heat shock proteins as a AQP2 interactors and have shown for hsc70 that this interaction is involved in AQP2 trafficking...
  23. pmc Bicarbonate-regulated adenylyl cyclase (sAC) is a sensor that regulates pH-dependent V-ATPase recycling
    Nuria Pastor-Soler
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    J Biol Chem 278:49523-9. 2003
    ..As sAC is expressed in other acid/base transporting epithelia, including kidney and choroid plexus, this cAMP-dependent signal transduction pathway may be a widespread mechanism that allows cells to sense and modulate extracellular pH...
  24. ncbi request reprint Functional role of the NPxxY motif in internalization of the type 2 vasopressin receptor in LLC-PK1 cells
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Am J Physiol Cell Physiol 285:C750-62. 2003
    ..However, this motif is not essential for the apical/basolateral sorting and polarized distribution of the V2R in LLC-PK1a cells or for adenylyl cyclase-mediated signal transduction...
  25. pmc Phosphorylation events and the modulation of aquaporin 2 cell surface expression
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Curr Opin Nephrol Hypertens 17:491-8. 2008
    ....
  26. ncbi request reprint Methyl-beta-cyclodextrin induces vasopressin-independent apical accumulation of aquaporin-2 in the isolated, perfused rat kidney
    Leileata M Russo
    Program in Membrane Biology, Division of Nephrology, Massachusetts General Hospital Harvard Medical School, Simches Research Center, 185 Cambridge St, Rm 8100, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 291:F246-53. 2006
    ....
  27. pmc Aldolase directly interacts with ARNO and modulates cell morphology and acidic vesicle distribution
    Maria Merkulova
    Program in Membrane Biology and Nephrology Division, Center for Systems Biology, Simches Research Center, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 300:C1442-55. 2011
    ....
  28. pmc Altered V-ATPase expression in renal intercalated cells isolated from B1 subunit-deficient mice by fluorescence-activated cell sorting
    Luca Vedovelli
    Center for Systems Biology, Program in Membrane Biology, Division of Nephrology, Massachusetts General Hospitaland Harvard Medical School, Boston, Massachusetts, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 304:F522-32. 2013
    ..We conclude that under baseline conditions B2 upregulation compensates for the lack of B1 and is sufficient to maintain basal acid-base homeostasis, even when other V-ATPase subunits are downregulated...
  29. pmc Visualizing microtubule-dependent vasopressin type 2 receptor trafficking using a new high-affinity fluorescent vasopressin ligand
    Sylvia Chen
    Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Endocrinology 152:3893-904. 2011
    ..We conclude that the new VP(TMR) ligand is suitable for dissecting V2R and VP internalization and trafficking in cells, and that V2R trafficking and down-regulation is an MT-dependent mechanism...
  30. pmc Vasopressin-induced differential stimulation of AQP4 splice variants regulates the in-membrane assembly of orthogonal arrays
    Alfred N Van Hoek
    Simches Research Center, 8 208, 185 Cambridge St, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 296:F1396-404. 2009
    ....
  31. pmc A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2
    Paula Nunes
    Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 295:C1476-87. 2008
    ..These results support the idea that, in addition to its role in reducing AQP2 endocytosis, VP increases AQP2 exocytosis...
  32. pmc Effects of the renal medullary pH and ionic environment on vasopressin binding and signaling
    Elena A Zalyapin
    Program in Membrane Biology and Nephrology Division, MGH Center for Systems Biology, Boston, Massachusetts 02114, USA
    Kidney Int 74:1557-67. 2008
    ..While the gradient is important for urinary concentration, it may also play a substantial role in fine-tuning of the vasopressin response through the V2R...
  33. pmc Proteomic analysis of V-ATPase-rich cells harvested from the kidney and epididymis by fluorescence-activated cell sorting
    Nicolas Da Silva
    Harvard Medical School, Boston, MA, USA
    Am J Physiol Cell Physiol 298:C1326-42. 2010
    ..These proteomic databases provide a framework for comprehensive future analysis of the common and distinct functions of V-ATPase-B1-expressing cells in the kidney and epididymis...
  34. ncbi request reprint Postnatal expression of aquaporins in epithelial cells of the rat epididymis
    Nicolas Da Silva
    Massachusetts General Hospital, Program in Membrane Biology Nephrology Division, Boston, Massachusetts 02114, USA
    Biol Reprod 74:427-38. 2006
    ..AQPs may be involved not only in the transepithelial transport of water in the epididymis but also in the postnatal development of this organ, as suggested by the differential expression of AQP2...
  35. pmc A dense network of dendritic cells populates the murine epididymis
    Nicolas Da Silva
    Program in Membrane Biology and Division of Nephrology, Center for Systems Biology, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge Street, CPZN 8 206, Boston, Massachusetts 02114 2790, USA
    Reproduction 141:653-63. 2011
    ..eDCs are, therefore, strategically positioned to regulate the complex interplay between immune tolerance and activation, a balance that is fundamental to male fertility...
  36. ncbi request reprint Expression of the 56-kDa B2 subunit isoform of the vacuolar H(+)-ATPase in proton-secreting cells of the kidney and epididymis
    Teodor G Paunescu
    Program in Membrane Biology Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 287:C149-62. 2004
    ..These findings indicate that in addition to its role in the acidification of intracellular organelles, the B2 isoform could also contribute to transepithelial proton secretion and the maintenance of acid-base homeostasis...
  37. ncbi request reprint Inhibition of endocytosis causes phosphorylation (S256)-independent plasma membrane accumulation of AQP2
    Hua Lu
    Program in Membrane Biology, Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 286:F233-43. 2004
    ....
  38. pmc Vasopressin induces apical expression of caveolin in rat kidney collecting duct principal cells
    Teodor G Paunescu
    Program in Membrane Biology Division of Nephrology, Massachusetts General Hospital, Simches Research Center, 185 Cambridge St, CPZN8150, Boston, MA 02114
    Am J Physiol Renal Physiol 305:F1783-95. 2013
    ..Importantly, the absence of apical caveolae indicates that while Cavs may have an as yet undetermined role in vasopressin-regulated signaling processes, this is probably unrelated to AQP2 internalization by caveolae. ..
  39. doi request reprint Circulating aldosterone induces the apical accumulation of the proton pumping V-ATPase and increases proton secretion in clear cells in the caput epididymis
    Jeremy W Roy
    Center for Systems Biology Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts and
    Am J Physiol Cell Physiol 305:C436-46. 2013
    ..This study, therefore, identifies aldosterone as an active member of the RAAS for the regulation of luminal acidification in the proximal epididymis. ..
  40. pmc Aquaporin 2 promotes cell migration and epithelial morphogenesis
    Ying Chen
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Simches Research Center, 185 Cambridge Street, Boston, MA 02114, USA
    J Am Soc Nephrol 23:1506-17. 2012
    ..In summary, these data suggest that the water channel AQP2 interacts with integrins to promote renal epithelial cell migration, contributing to the structural and functional integrity of the mammalian kidney...
  41. pmc Bypassing vasopressin receptor signaling pathways in nephrogenic diabetes insipidus
    Richard Bouley
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Semin Nephrol 28:266-78. 2008
    ....
  42. pmc Transepithelial projections from basal cells are luminal sensors in pseudostratified epithelia
    Winnie Wai Chi Shum
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, MA 02114, USA
    Cell 135:1108-17. 2008
    ..We propose a paradigm in which basal cells scan and sense the luminal environment of pseudostratified epithelia and modulate epithelial function by a mechanism involving crosstalk with other epithelial cells...
  43. ncbi request reprint Differential expression and targeting of endogenous Arf1 and Arf6 small GTPases in kidney epithelial cells in situ
    Jaafar El-Annan
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Boston, MA 02129 2020, USA
    Am J Physiol Cell Physiol 286:C768-78. 2004
    ..The results provide a framework on which to base and interpret future studies on the role of Arf GTPases in the multitude of cellular trafficking events that occur in renal tubular epithelial cells...
  44. pmc Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones
    NURIA M PASTOR-SOLER
    Program in Membrane Biology and Nephrology Division, Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Reproduction 139:613-21. 2010
    ..Our data show that expression of AQP9 in the developing rat epididymis is downregulated by neonatal DES, GNRHa, EE, and flutamide, and that the effects mediated by estrogens can be prevented by testosterone administration...
  45. pmc Loss of the V-ATPase B1 subunit isoform expressed in non-neuronal cells of the mouse olfactory epithelium impairs olfactory function
    Teodor G Paunescu
    MGH Center for Systems Biology, Division of Nephrology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 7:e45395. 2012
    ..We conclude that V-ATPase-mediated H(+) secretion in the olfactory epithelium is required for optimal olfactory function...
  46. pmc V-ATPase expression in the mouse olfactory epithelium
    Teodor G Paunescu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital, Massachusetts 02114, USA
    Am J Physiol Cell Physiol 295:C923-30. 2008
    ..These findings suggest that V-ATPase may be involved in proton secretion in the OE and, as such, may be important for the pH homeostasis of the neuroepithelial mucous layer and/or for signal transduction in CO(2) detection...
  47. pmc The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells
    Hua Jenny Lu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 295:F290-4. 2008
    ..The potential role of S261 phosphorylation/dephosphorylation in vasopressin action remains to be determined...
  48. doi request reprint Regulation of luminal acidification by the V-ATPase
    Sylvie Breton
    Program in Membrane Biology, Center for Systems Biology, Nephrology Division, Massachusetts General Hospital, Boston, MA, USA
    Physiology (Bethesda) 28:318-29. 2013
    ..This review focuses on the regulation of V-ATPase-dependent luminal acidification in renal intercalated cells and epididymal clear cells, which are key players in these physiological processes. ..
  49. pmc High resolution helium ion scanning microscopy of the rat kidney
    William L Rice
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS ONE 8:e57051. 2013
    ..Its use in future studies should allow the study of fine cellular details and provide significant advances in our understanding of cell surface structures and membrane organization...
  50. doi request reprint Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease
    Kevin C Miranda
    Division of Nephrology, Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Kidney Int 78:191-9. 2010
    ..Our study provides an experimental strategy for the routine isolation and use of urinary microvesicles as a novel and non-invasive source of nucleic acids to further renal disease biomarker discovery...
  51. pmc Regulation of luminal acidification in the male reproductive tract via cell-cell crosstalk
    Winnie W C Shum
    Center for Systems Biology, Program in Membrane Biology, Nephrology Division, Massachusetts General Hospital, Boston, MA 02114, USA
    J Exp Biol 212:1753-61. 2009
    ..Thus, a complex interplay between the different cell types present in the epithelium leads to activation of the luminal acidifying capacity of the epididymis, a process that is crucial for sperm maturation and storage...
  52. pmc Impaired tubular uptake explains albuminuria in early diabetic nephropathy
    Leileata M Russo
    Program in Membrane Biology, Division of Nephrology, Massachusetts General Hospital Harvard Medical School, Simches Research Center, Boston, MA 02114, USA
    J Am Soc Nephrol 20:489-94. 2009
    ..In conclusion, in the early stages of diabetic nephropathy, the PT plays a major role in the development of albuminuria, which may be preceded by peptideuria...
  53. ncbi request reprint New insights into the regulation of V-ATPase-dependent proton secretion
    Sylvie Breton
    Program in Membrane Biology, Nephrology Division, Massachusetts General Hospital, and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114 2790, USA
    Am J Physiol Renal Physiol 292:F1-10. 2007
    ..Modulation of the V-ATPase-rich cell phenotype and the pathophysiology of the V-ATPase in humans and experimental animals are also discussed...
  54. pmc Deletion of Cavin/PTRF causes global loss of caveolae, dyslipidemia, and glucose intolerance
    Libin Liu
    Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
    Cell Metab 8:310-7. 2008
    ..Our results underscore the multiorgan role of caveolae in metabolic regulation and the obligate presence of Cavin for caveolae formation...
  55. pmc Angiotensin II and hypertonicity modulate proximal tubular aquaporin 1 expression
    Richard Bouley
    Center for Systems Biology, Program in Membrane Biology, Nephrology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 297:F1575-86. 2009
    ....
  56. ncbi request reprint Segmental and cellular expression of aquaporins in the male excurrent duct
    Nicolas Da Silva
    Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02114 2790, USA
    Biochim Biophys Acta 1758:1025-33. 2006
    ..The present article reviews our current knowledge regarding the distribution and function of aquaporins in the male excurrent duct...
  57. pmc Inhibitory effects of Robo2 on nephrin: a crosstalk between positive and negative signals regulating podocyte structure
    Xueping Fan
    Renal Section, Department of Medicine, Boston University Medical Center, Boston, MA 02118, USA
    Cell Rep 2:52-61. 2012
    ..These results suggest that Robo2 signaling acts as a negative regulator on nephrin to influence podocyte foot process architecture...
  58. ncbi request reprint Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens
    Nuria Pastor-Soler
    Renal Unit and Program in Membrane Biology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Biol Reprod 66:1716-22. 2002
    ..These data indicate that the expression of AQP9, a likely candidate for apical transepithelial fluid and solute transport in several regions of the male reproductive tract, is modulated by androgens in the adult rat epididymis...
  59. ncbi request reprint Detection of ClC-3 and ClC-5 in epididymal epithelium: immunofluorescence and RT-PCR after LCM
    Corinne Isnard-Bagnis
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 284:C220-32. 2003
    ..ClC-5 might facilitate endosome trafficking in the epididymis, as has been proposed in the kidney...
  60. ncbi request reprint The ins and outs of aquaporin-2 trafficking
    Dennis Brown
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital, Charlestown 02129, USA
    Am J Physiol Renal Physiol 284:F893-901. 2003
    ..Understanding cAMP-independent mechanisms for membrane insertion of AQP2 is especially relevant to the therapeutic bypassing of the mutated, dysfunctional vasopressin receptor in patients with X-linked nephrogenic diabetes insipidus...
  61. doi request reprint The soluble transforming growth factor-beta receptor: advantages and applications
    Leileata M Russo
    Program in Membrane Biology, Center for Systems Biology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Int J Biochem Cell Biol 41:472-6. 2009
    ..Here we discuss the design, use and advantages of the highly specific TGF-beta binding molecule, the soluble human TGF-beta receptor (sTbetaR.Fc) as a TGF-beta sequestering agent...
  62. ncbi request reprint Physiological importance of endosomal acidification: potential role in proximal tubulopathies
    Vladimir Marshansky
    Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Boston 02129 2020, USA
    Curr Opin Nephrol Hypertens 11:527-37. 2002
    ....
  63. pmc Is caveolin involved in normal proximal tubule function? Presence in model PT systems but absence in situ
    Zhenjie Zhuang
    Massachusetts General Hospital, Center for Systems Biology, Simches Research Bldg, Massachusetts General Hospital and Harvard Medical School, 185 Cambridge St, CPZN 8150, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 300:F199-206. 2011
    ....
  64. pmc Commensal-associated molecular patterns induce selective toll-like receptor-trafficking from apical membrane to cytoplasmic compartments in polarized intestinal epithelium
    Elke Cario
    Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Am J Pathol 160:165-73. 2002
    ..The results of this dynamic epithelial surveillance can then be conveyed to the underlying cell populations of the lamina propria via these innate immune pattern recognition receptors...
  65. ncbi request reprint Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal degradation pathway
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital East, 149 13th St, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 288:C1390-401. 2005
    ..This degradative pathway may be an adaptive response to allow receptor-ligand association in the hypertonic and acidic environment of the renal medulla...
  66. pmc AQP2 is necessary for vasopressin- and forskolin-mediated filamentous actin depolymerization in renal epithelial cells
    Naofumi Yui
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Biol Open 1:101-8. 2012
    ..This is the first report showing that VP/FK mediated F-actin depolymerization is dependent on AQP2 protein expression in renal epithelial cells, and that this is not dependent on the polarity of AQP2 membrane insertion...
  67. ncbi request reprint Aquaporin-2 localization in clathrin-coated pits: inhibition of endocytosis by dominant-negative dynamin
    Tian Xiao Sun
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 282:F998-1011. 2002
    ..We propose that the IMP clusters previously associated with vasopressin action represent sites of dynamin-dependent, clathrin-mediated endocytosis in which AQP2 is concentrated before internalization...
  68. ncbi request reprint Imaging protein trafficking
    Dennis Brown
    Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass 02114, USA
    Nephron Exp Nephrol 103:e55-61. 2006
    ..This review briefly introduces and discusses some of the systems and methodologies that are available from several manufacturers, including laser scanning and spinning disk confocal microscopy, and total internal reflectance microscopy...
  69. ncbi request reprint Evidence for a role of transforming growth factor (TGF)-beta1 in the induction of postglomerular albuminuria in diabetic nephropathy: amelioration by soluble TGF-beta type II receptor
    Leileata M Russo
    Program in Membrane Biology, Division of Nephrology, Department of Medicine, Massachusetts General Hospital Harvard Medical School, Simches Research Center, 185 Cambridge Street, Boston, MA 02114, USA
    Diabetes 56:380-8. 2007
    ..Fc is a potential new agent for the treatment of fibrosis and albuminuria in diabetic nephropathy and may reduce albuminuria by reducing TGF-beta1-induced disruptions of renal proximal tubule cell uptake of albumin...
  70. pmc Hydrocortisone modulates cholera toxin endocytosis by regulating immature enterocyte plasma membrane phospholipids
    Lei Lu
    Developmental Gastroenterology Laboratory, Massachusetts General Hospital for Children, Boston, Massachusetts, USA
    Gastroenterology 135:185-193.e1. 2008
    ..Our aim was to characterize the developmental difference in cholera toxin (CT)-GM1-mediated endocytosis...
  71. pmc Dragon enhances BMP signaling and increases transepithelial resistance in kidney epithelial cells
    Yin Xia
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Am Soc Nephrol 21:666-77. 2010
    ..Taken together, these results suggest that Dragon may enhance BMP signaling in renal tubular epithelial cells and maintain normal renal physiology...
  72. ncbi request reprint Nephrin dissociates from actin, and its expression is reduced in early experimental membranous nephropathy
    Huaiping Yuan
    Renal Section and Department of Medicine, Evans Biomedical Research Center, Boston University Medical Center, 650 Albany Street, Boston, MA 02118, USA
    J Am Soc Nephrol 13:946-56. 2002
    ..These events may underlie the loss of podocyte barrier function in membranous nephropathy...
  73. ncbi request reprint In vivo molecular probing of cellular compartments with gold nanoparticles and nanoaggregates
    Janina Kneipp
    Wellman Center for Photomedicine, Harvard University, Medical School, Boston, Massachusetts 02114, USA
    Nano Lett 6:2225-31. 2006
    ..The results have implications for medical and biotechnology applications of SERS nanosensors in cells...
  74. ncbi request reprint Antigen retrieval reveals widespread basolateral expression of syntaxin 3 in renal epithelia
    Sylvie Breton
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 282:F523-9. 2002
    ..The exclusive basolateral location of syntaxin 3 in situ, however, contrasts with the apical location of this SNARE protein in some kidney epithelial cells in culture...
  75. pmc TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function
    Jochen Reiser
    Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02129, USA
    Nat Genet 37:739-44. 2005
    ..Two of the TRPC6 mutants had increased current amplitudes. These data show that TRPC6 channel activity at the slit diaphragm is essential for proper regulation of podocyte structure and function...
  76. ncbi request reprint Identification of cytoskeletal regulatory proteins required for efficient phagocytosis in Drosophila
    Alan M Pearson
    Department of Pediatrics, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Microbes Infect 5:815-24. 2003
    ..This study demonstrates that Drosophila provides a viable model system in which to dissect the complex interactions that regulate phagocytosis...