D Brown

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc An extended nomenclature for mammalian V-ATPase subunit genes and splice variants
    Kevin C Miranda
    Program in Membrane Biology and Division of Nephrology, Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 5:e9531. 2010
  2. ncbi Role of V-ATPase-rich cells in acidification of the male reproductive tract
    D Brown
    Renal Unit, Massachusetts General Hospital, Boston, USA
    J Exp Biol 200:257-62. 1997
  3. ncbi Mitochondria-rich, proton-secreting epithelial cells
    D Brown
    Renal Unit, Massachusetts General Hospital, Boston 02129, USA
    J Exp Biol 199:2345-58. 1996
  4. ncbi H(+)V-ATPase-dependent luminal acidification in the kidney collecting duct and the epididymis/vas deferens: vesicle recycling and transcytotic pathways
    D Brown
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Boston, MA 02129, USA
    J Exp Biol 203:137-45. 2000
  5. ncbi Targeting of membrane transporters in renal epithelia: when cell biology meets physiology
    D Brown
    Program in Membrane Biology, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 278:F192-201. 2000
  6. ncbi Sorting proteins to their target membranes
    D Brown
    Renal Unit, Massachusetts General Hospital, USA
    Kidney Int 57:816-24. 2000
  7. ncbi Imaging protein trafficking
    Dennis Brown
    Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass 02114, USA
    Nephron Exp Nephrol 103:e55-61. 2006
  8. ncbi The ins and outs of aquaporin-2 trafficking
    Dennis Brown
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital, Charlestown 02129, USA
    Am J Physiol Renal Physiol 284:F893-901. 2003
  9. ncbi Expression of gp330 and gp330/alpha 2-macroglobulin receptor-associated protein in renal tubular differentiation
    M Abbate
    Renal Unit, Massachusetts General Hospital, Boston
    J Am Soc Nephrol 4:2003-15. 1994
  10. pmc Detection of two forms of GP330. Their role in Heymann nephritis
    D R Bachinsky
    Department of Pathology, Massachusetts General Hospital, Charlestown 02129
    Am J Pathol 143:598-611. 1993

Collaborators

Detail Information

Publications120 found, 100 shown here

  1. pmc An extended nomenclature for mammalian V-ATPase subunit genes and splice variants
    Kevin C Miranda
    Program in Membrane Biology and Division of Nephrology, Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 5:e9531. 2010
    ..It is envisioned this catalogue will form a new platform on which future studies into tissue- and organelle-specific V-ATPase expression, localization and function can be based...
  2. ncbi Role of V-ATPase-rich cells in acidification of the male reproductive tract
    D Brown
    Renal Unit, Massachusetts General Hospital, Boston, USA
    J Exp Biol 200:257-62. 1997
    ....
  3. ncbi Mitochondria-rich, proton-secreting epithelial cells
    D Brown
    Renal Unit, Massachusetts General Hospital, Boston 02129, USA
    J Exp Biol 199:2345-58. 1996
    ..This review emphasizes important structural and functional features of proton-secreting cells, describes the tissue distribution of these cells and discusses the known functions of these cells in their respective epithelia...
  4. ncbi H(+)V-ATPase-dependent luminal acidification in the kidney collecting duct and the epididymis/vas deferens: vesicle recycling and transcytotic pathways
    D Brown
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Boston, MA 02129, USA
    J Exp Biol 203:137-45. 2000
    ..We propose that the 'polarity reversal' of the H(+)V-ATPase in B-intercalated cells is mediated by a physiologically regulated transcytotic pathway that may be similar to that existing in some other cell types...
  5. ncbi Targeting of membrane transporters in renal epithelia: when cell biology meets physiology
    D Brown
    Program in Membrane Biology, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 278:F192-201. 2000
    ..Examples of these events in renal epithelial cells are highlighted throughout this review and are related to the physiology of the kidney...
  6. ncbi Sorting proteins to their target membranes
    D Brown
    Renal Unit, Massachusetts General Hospital, USA
    Kidney Int 57:816-24. 2000
    ..In some cases, the mutated protein is retained and degraded intracellularly, while in others it may not be delivered to the cell surface after the appropriate physiological stimulation...
  7. ncbi Imaging protein trafficking
    Dennis Brown
    Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass 02114, USA
    Nephron Exp Nephrol 103:e55-61. 2006
    ..This review briefly introduces and discusses some of the systems and methodologies that are available from several manufacturers, including laser scanning and spinning disk confocal microscopy, and total internal reflectance microscopy...
  8. ncbi The ins and outs of aquaporin-2 trafficking
    Dennis Brown
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital, Charlestown 02129, USA
    Am J Physiol Renal Physiol 284:F893-901. 2003
    ..Understanding cAMP-independent mechanisms for membrane insertion of AQP2 is especially relevant to the therapeutic bypassing of the mutated, dysfunctional vasopressin receptor in patients with X-linked nephrogenic diabetes insipidus...
  9. ncbi Expression of gp330 and gp330/alpha 2-macroglobulin receptor-associated protein in renal tubular differentiation
    M Abbate
    Renal Unit, Massachusetts General Hospital, Boston
    J Am Soc Nephrol 4:2003-15. 1994
    ....
  10. pmc Detection of two forms of GP330. Their role in Heymann nephritis
    D R Bachinsky
    Department of Pathology, Massachusetts General Hospital, Charlestown 02129
    Am J Pathol 143:598-611. 1993
    ..The complexes that are shed in Heymann nephritis contain either a portion of gp330 cleaved from the full-length molecule or a form of gp330 that lacks the cytoplasmic domain...
  11. pmc Loss of antigens associated with the apical endocytotic pathway in proximal tubules from rats with heymann nephritis
    E J Gutmann
    Department of Pathology, Massachusetts General Hospital, Boston
    Am J Pathol 138:1243-55. 1991
    ..The results also suggest that antibody-antigen complexes may be shed from the plasma membrane in both the glomerulus and the proximal tubule in this disease...
  12. ncbi Cold-induced microtubule disruption and relocalization of membrane proteins in kidney epithelial cells
    S Breton
    Renal Unit, Massachusetts General Hospital and Harvard Medical School, Boston 02129, USA
    J Am Soc Nephrol 9:155-66. 1998
    ..Thus, cold preservation of tissues might impair, at least transiently, the polarized membrane expression and function of some transport proteins in renal epithelial cells...
  13. ncbi Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract
    S Breton
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Charlestown 02129, USA
    Am J Physiol Renal Physiol 278:F717-25. 2000
    ..These results suggest that H(+)-ATPase is actively endocytosed and exocytosed in proton-secreting cells of the epididymis and vas deferens and that net proton secretion requires the participation of the v-SNARE cellubrevin...
  14. ncbi Colchicine-induced redistribution of an apical membrane glycoprotein (gp330) in proximal tubules
    E J Gutmann
    Department of Pathology, Massachusetts General Hospital, Boston 02114
    Am J Physiol 257:C397-407. 1989
    ..Our results indicate that although microtubules are involved in the accumulation of gp330-containing vesicles at the apical pole of the cell, other factors must be required for fusion with the plasma membrane to occur...
  15. ncbi Controversies in nephrology: response to 'renal albumin handling, facts, and artifacts'
    L M Russo
    Program in Membrane Biology, Renal Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Kidney Int 72:1195-7. 2007
    ....
  16. ncbi Relocalization of the V-ATPase B2 subunit to the apical membrane of epididymal clear cells of mice deficient in the B1 subunit
    Nicolas Da Silva
    Massachusetts General Hospital, Harvard Medical School, Program in Membrane Biology, Nephrology Div, 185 Cambridge St, CPZN 8150, Boston, MA 02114 2790, USA
    Am J Physiol Cell Physiol 293:C199-210. 2007
    ..This relocation compensates for the absence of functional B1 and maintains the luminal pH in an acidic range that is compatible with fertility...
  17. ncbi Aquaporin-4 is expressed in basolateral membranes of proximal tubule S3 segments in mouse kidney
    A N van Hoek
    Program in Membrane Biology Renal Unit and Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 278:F310-6. 2000
    ..The functional significance of the apparent species-dependent expression of AQP4 in proximal tubules is unknown, but may relate to physiological differences between rats and mice...
  18. ncbi The normal kidney filters nephrotic levels of albumin retrieved by proximal tubule cells: retrieval is disrupted in nephrotic states
    L M Russo
    Program in Membrane Biology, Renal Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Kidney Int 71:504-13. 2007
    ..Thus, restoration of the defective endocytotic and processing function of PT epithelial cells might represent an effective strategy to limit urinary albumin loss, at least in some types of nephrotic syndrome...
  19. ncbi The mercurial insensitive water channel (AQP-4) forms orthogonal arrays in stably transfected Chinese hamster ovary cells
    B Yang
    Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, California 94143 0521, USA
    J Biol Chem 271:4577-80. 1996
    ..These results provide direct evidence that a molecular water channel can spontaneously assemble in regular arrays...
  20. ncbi Acidification of the male reproductive tract by a proton pumping (H+)-ATPase
    S Breton
    Renal Unit, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    Nat Med 2:470-2. 1996
    ..This acidification mechanism is a potential target for novel strategies aimed at modulating the acidification capacity of parts of the male reproductive tract and, therefore, in regulating male fertility...
  21. ncbi Aquaporin 2 (AQP2) and vasopressin type 2 receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in 'endocytosis-resistant' membrane domains after vasopressin treatment
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Biol Cell 98:215-32. 2006
    ..In the present study, we further investigated AQP2 accumulation at the cell surface, and compared it with V2R (VP type 2 receptor) trafficking using cells that express epitope-tagged AQP2 and V2R...
  22. ncbi Inhibition of endocytosis causes phosphorylation (S256)-independent plasma membrane accumulation of AQP2
    Hua Lu
    Program in Membrane Biology, Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 286:F233-43. 2004
    ....
  23. pmc Segmental expression of the bradykinin type 2 receptor in rat efferent ducts and epididymis and its role in the regulation of aquaporin 9
    C Belleannee
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Biol Reprod 80:134-43. 2009
    ..This effect was inhibited by BAPTA-AM, demonstrating the participation of calcium in this process. This study, therefore, identifies BK as an important regulator of AQP9...
  24. ncbi Remodeling the cellular profile of collecting ducts by chronic carbonic anhydrase inhibition
    C Bagnis
    Program in Membrane Biology, Massachusetts General Hospital, Boston, Massachusetts 02120, USA
    Am J Physiol Renal Physiol 280:F437-48. 2001
    ....
  25. ncbi Location of gp330/alpha 2-m receptor-associated protein (alpha 2-MRAP) and its binding sites in kidney: distribution of endogenous alpha 2-MRAP is modified by tissue processing
    M Abbate
    Renal Unit, Massachusetts General Hospital, Boston
    Eur J Cell Biol 61:139-49. 1993
    ....
  26. ncbi Role of megalin (gp330) in transcytosis of thyroglobulin by thyroid cells. A novel function in the control of thyroid hormone release
    M Marino
    Pathology Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    J Biol Chem 275:7125-37. 2000
    ..Tg transcytosis is a novel function of megalin, which usually transports ligands to lysosomes. Megalin-mediated transcytosis may regulate the extent of thyroid hormone release...
  27. pmc Induction of Heymann nephritis with a gp330/megalin fusion protein
    R Raychowdhury
    Department of Pathology, Massachusetts General Hospital, Charlestown 02129, USA
    Am J Pathol 148:1613-23. 1996
    ..The results show that the region of gp330 spanning amino acid residues 1114 to 1250 contains peptides capable of inducing pathogenic antibodies of Heymann nephritis without a contributory role of RAP...
  28. ncbi Absence of aquaporin-4 water channels from kidneys of the desert rodent Dipodomys merriami merriami
    Y Huang
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital and Harvard Medical School, Boston 02114, USA
    Am J Physiol Renal Physiol 280:F794-802. 2001
    ....
  29. ncbi Aquaporin 9 expression along the male reproductive tract
    N Pastor-Soler
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Biol Reprod 65:384-93. 2001
    ....
  30. ncbi Distinct expression patterns of different subunit isoforms of the V-ATPase in the rat epididymis
    C Pietrement
    Program in Membrane Biology, Massachusetts General Hospital, Boston, MA 02114, USA
    Biol Reprod 74:185-94. 2006
    ..In principal cells, the ATP6V0D1 isoform may have a physiological function that is distinct from its role in proton transport via the V-ATPase complex...
  31. ncbi Modulation of the actin cytoskeleton via gelsolin regulates vacuolar H+-ATPase recycling
    Valerie Beaulieu
    Program in Membrane Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    J Biol Chem 280:8452-63. 2005
    ..Therefore, modulation of the actin cortex by this severing and capping protein may represent a common mechanism by which these cells regulate their rate of proton secretion...
  32. pmc Acute hypertonicity alters aquaporin-2 trafficking and induces a MAPK-dependent accumulation at the plasma membrane of renal epithelial cells
    Udo Hasler
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts 02114 2790, USA
    J Biol Chem 283:26643-61. 2008
    ..This may have important implications on adaptational processes governing transcellular water flux and/or cell survival under extreme conditions of hypertonicity...
  33. ncbi Membrane organization and function of M1 and M23 isoforms of aquaporin-4 in epithelial cells
    Claudia Silberstein
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 287:F501-11. 2004
    ....
  34. ncbi Association of soluble adenylyl cyclase with the V-ATPase in renal epithelial cells
    Teodor G Paunescu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 294:F130-8. 2008
    ..This suggests that these enzymes have a close association and could be part of a protein complex that is involved in regulating renal distal proton secretion...
  35. ncbi Postnatal development of H+ ATPase (proton-pump)-rich cells in rat epididymis
    S Breton
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital East, Charlestown 02129, USA
    Histochem Cell Biol 111:97-105. 1999
    ..These results demonstrate that differentiated subpopulations of proton-secreting epithelial cells appear early during epididymal development, and that the induction of H+ ATPase in these cells occurs prior to sexual maturation...
  36. pmc cAMP stimulates apical V-ATPase accumulation, microvillar elongation, and proton extrusion in kidney collecting duct A-intercalated cells
    Teodor G Paunescu
    MGH Center for Systems Biology, Program in Membrane Biology, and Division of Nephrology, Massachusetts General Hospital, and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 298:F643-54. 2010
    ....
  37. ncbi Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra)
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Rm 8207, Massachusetts General Hospital East, 149 13th St Charlestown, MA 02129, USA
    Am J Physiol Renal Physiol 288:F1103-12. 2005
    ....
  38. ncbi Aquaporin-2 localization in clathrin-coated pits: inhibition of endocytosis by dominant-negative dynamin
    Tian Xiao Sun
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 282:F998-1011. 2002
    ..We propose that the IMP clusters previously associated with vasopressin action represent sites of dynamin-dependent, clathrin-mediated endocytosis in which AQP2 is concentrated before internalization...
  39. ncbi Renal concentrating defect in mice lacking group IV cytosolic phospholipase A(2)
    P Downey
    Medical and Anesthesia Services, Massachusetts General Hospital, Charlestown, 02129, USA
    Am J Physiol Renal Physiol 280:F607-18. 2001
    ..Thus mice lacking cPLA(2) develop an age-related defect in renal concentration that may be related to abnormal trafficking and/or folding of AQP1 in the proximal tubule, implicating cPLA(2) in these processes...
  40. ncbi Transcytosis of retinol-binding protein across renal proximal tubule cells after megalin (gp 330)-mediated endocytosis
    M Marino
    Pathology Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts
    J Am Soc Nephrol 12:637-48. 2001
    ....
  41. ncbi Depletion of intercalated cells from collecting ducts of carbonic anhydrase II-deficient (CAR2 null) mice
    S Breton
    Renal Unit, Massachusetts General Hospital, Boston, USA
    Am J Physiol 269:F761-74. 1995
    ..Elucidation of the mechanism(s) responsible for this phenotype will be of importance in understanding the origin and development of intercalated cells in the kidney...
  42. pmc Role of purinergic signaling pathways in V-ATPase recruitment to apical membrane of acidifying epididymal clear cells
    Clémence Belleannee
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Am J Physiol Cell Physiol 298:C817-30. 2010
    ....
  43. pmc Tetrameric assembly of CHIP28 water channels in liposomes and cell membranes: a freeze-fracture study
    J M Verbavatz
    Renal Unit, Massachusetts General Hospital
    J Cell Biol 123:605-18. 1993
    ..5 nm. These results provide a morphological signature for CHIP28 water channels and evidence for a tetrameric assembly of CHIP28 monomers in reconstituted proteoliposomes and cell membranes...
  44. ncbi V-ATPase interacts with ARNO and Arf6 in early endosomes and regulates the protein degradative pathway
    Andres Hurtado-Lorenzo
    Program in Membrane Biology and Nephrology Division, Richard Simches Research Center, Massachusetts General Hospital and Department of Medicine, Harvard Medical School, Boston, MA 02114, USA
    Nat Cell Biol 8:124-36. 2006
    ....
  45. pmc Bicarbonate-regulated adenylyl cyclase (sAC) is a sensor that regulates pH-dependent V-ATPase recycling
    Nuria Pastor-Soler
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    J Biol Chem 278:49523-9. 2003
    ..As sAC is expressed in other acid/base transporting epithelia, including kidney and choroid plexus, this cAMP-dependent signal transduction pathway may be a widespread mechanism that allows cells to sense and modulate extracellular pH...
  46. ncbi Alkaline pH- and cAMP-induced V-ATPase membrane accumulation is mediated by protein kinase A in epididymal clear cells
    NURIA M PASTOR-SOLER
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, A915 Scaife Hall, 3550 Terrace St, Pittsburgh, PA 15263, USA
    Am J Physiol Cell Physiol 294:C488-94. 2008
    ..We conclude that direct stimulation of PKA activity by cAMP is necessary and sufficient for the alkaline pH-induced accumulation of V-ATPase in clear cell apical microvilli...
  47. ncbi Differential expression and targeting of endogenous Arf1 and Arf6 small GTPases in kidney epithelial cells in situ
    Jaafar El-Annan
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Boston, MA 02129 2020, USA
    Am J Physiol Cell Physiol 286:C768-78. 2004
    ..The results provide a framework on which to base and interpret future studies on the role of Arf GTPases in the multitude of cellular trafficking events that occur in renal tubular epithelial cells...
  48. ncbi Antigen retrieval reveals widespread basolateral expression of syntaxin 3 in renal epithelia
    Sylvie Breton
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 282:F523-9. 2002
    ..The exclusive basolateral location of syntaxin 3 in situ, however, contrasts with the apical location of this SNARE protein in some kidney epithelial cells in culture...
  49. ncbi Subcellular localization of presenilin 2 endoproteolytic C-terminal fragments
    T L Tekirian
    Genetics and Aging Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Brain Res Mol Brain Res 96:14-20. 2001
    ..In summary, these data suggest that the two presenilin 2 CTFs have different patterns of subcellular localization and that the N141I PS2 mutation alters the localization pattern of the PS2 caspase fragment...
  50. ncbi A plasma membrane proton ATPase in specialized cells of rat epididymis
    D Brown
    Renal Unit, Massachusetts General Hospital, Boston
    Am J Physiol 263:C913-6. 1992
    ..By analogy with kidney intercalated cells, these cell types may be specialized for acid secretion in the epididymis...
  51. ncbi Intra-endosomal pH-sensitive recruitment of the Arf-nucleotide exchange factor ARNO and Arf6 from cytoplasm to proximal tubule endosomes
    B Maranda
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, and Department of Medicine, Harvard Medical School, Boston, Massachusetts, 02129 2020, USA
    J Biol Chem 276:18540-50. 2001
    ..This mechanism may play an important role in the pH-dependent regulation of receptor-mediated endocytosis in proximal tubules in situ...
  52. ncbi Immunolocalization and tissue-specific splicing of AE2 anion exchanger in mouse kidney
    A K Stuart-Tilley
    Molecular Medicine Unit, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    J Am Soc Nephrol 9:946-59. 1998
    ..Unlike in rat, the mouse AE2c2 mRNA splice variant encoded a polypeptide with a novel predicted N-terminal amino acid sequence...
  53. ncbi The B1 subunit of the H+ATPase is a PDZ domain-binding protein. Colocalization with NHE-RF in renal B-intercalated cells
    S Breton
    Renal Unit and Program in Membrane Biology and the Molecular Neurogenetics Unit, Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA
    J Biol Chem 275:18219-24. 2000
    ..The colocalization of NHE-RF and H(+)ATPase in B- but not A-intercalated cells suggests a role in generating, maintaining, or modulating the variable H(+)ATPase polarity that characterizes the B-cell phenotype...
  54. pmc V-ATPase expression in the mouse olfactory epithelium
    Teodor G Paunescu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital, Massachusetts 02114, USA
    Am J Physiol Cell Physiol 295:C923-30. 2008
    ..These findings suggest that V-ATPase may be involved in proton secretion in the OE and, as such, may be important for the pH homeostasis of the neuroepithelial mucous layer and/or for signal transduction in CO(2) detection...
  55. pmc Phosphorylation events and the modulation of aquaporin 2 cell surface expression
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Curr Opin Nephrol Hypertens 17:491-8. 2008
    ....
  56. pmc A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2
    Paula Nunes
    Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 295:C1476-87. 2008
    ..These results support the idea that, in addition to its role in reducing AQP2 endocytosis, VP increases AQP2 exocytosis...
  57. ncbi Postnatal expression of aquaporins in epithelial cells of the rat epididymis
    Nicolas Da Silva
    Massachusetts General Hospital, Program in Membrane Biology Nephrology Division, Boston, Massachusetts 02114, USA
    Biol Reprod 74:427-38. 2006
    ..AQPs may be involved not only in the transepithelial transport of water in the epididymis but also in the postnatal development of this organ, as suggested by the differential expression of AQP2...
  58. pmc Bypassing vasopressin receptor signaling pathways in nephrogenic diabetes insipidus
    Richard Bouley
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Semin Nephrol 28:266-78. 2008
    ....
  59. pmc Hydrocortisone modulates cholera toxin endocytosis by regulating immature enterocyte plasma membrane phospholipids
    Lei Lu
    Developmental Gastroenterology Laboratory, Massachusetts General Hospital for Children, Boston, Massachusetts, USA
    Gastroenterology 135:185-193.e1. 2008
    ..Our aim was to characterize the developmental difference in cholera toxin (CT)-GM1-mediated endocytosis...
  60. ncbi Methyl-beta-cyclodextrin induces vasopressin-independent apical accumulation of aquaporin-2 in the isolated, perfused rat kidney
    Leileata M Russo
    Program in Membrane Biology, Division of Nephrology, Massachusetts General Hospital Harvard Medical School, Simches Research Center, 185 Cambridge St, Rm 8100, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 291:F246-53. 2006
    ....
  61. pmc The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells
    Hua Jenny Lu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 295:F290-4. 2008
    ..The potential role of S261 phosphorylation/dephosphorylation in vasopressin action remains to be determined...
  62. doi The soluble transforming growth factor-beta receptor: advantages and applications
    Leileata M Russo
    Program in Membrane Biology, Center for Systems Biology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Int J Biochem Cell Biol 41:472-6. 2009
    ..Here we discuss the design, use and advantages of the highly specific TGF-beta binding molecule, the soluble human TGF-beta receptor (sTbetaR.Fc) as a TGF-beta sequestering agent...
  63. ncbi Evidence for a role of transforming growth factor (TGF)-beta1 in the induction of postglomerular albuminuria in diabetic nephropathy: amelioration by soluble TGF-beta type II receptor
    Leileata M Russo
    Program in Membrane Biology, Division of Nephrology, Department of Medicine, Massachusetts General Hospital Harvard Medical School, Simches Research Center, 185 Cambridge Street, Boston, MA 02114, USA
    Diabetes 56:380-8. 2007
    ..Fc is a potential new agent for the treatment of fibrosis and albuminuria in diabetic nephropathy and may reduce albuminuria by reducing TGF-beta1-induced disruptions of renal proximal tubule cell uptake of albumin...
  64. pmc Calcitonin has a vasopressin-like effect on aquaporin-2 trafficking and urinary concentration
    Richard Bouley
    Massachusetts General Hospital, Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Simches Research Center, Room 8202, 185 Cambridge Street, Boston, MA 02114, USA
    J Am Soc Nephrol 22:59-72. 2011
    ..This suggests that calcitonin has a potential therapeutic use in nephrogenic diabetes insipidus...
  65. ncbi Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking
    Hua A J Lu
    Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 282:28721-32. 2007
    ..To summarize, we have identified the 70-kDa heat shock proteins as a AQP2 interactors and have shown for hsc70 that this interaction is involved in AQP2 trafficking...
  66. ncbi Compensatory membrane expression of the V-ATPase B2 subunit isoform in renal medullary intercalated cells of B1-deficient mice
    Teodor G Paunescu
    Div of Nephrology, Massachusetts General Hospital, 185 Cambridge St, CPZN 8150, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 293:F1915-26. 2007
    ..However, our results show no increase in cell surface V-ATPase activity in response to metabolic acidosis in ICs from these animals, consistent with their inability to appropriately acidify their urine under these conditions...
  67. pmc Effects of the renal medullary pH and ionic environment on vasopressin binding and signaling
    Elena A Zalyapin
    Program in Membrane Biology and Nephrology Division, MGH Center for Systems Biology, Boston, Massachusetts 02114, USA
    Kidney Int 74:1557-67. 2008
    ..While the gradient is important for urinary concentration, it may also play a substantial role in fine-tuning of the vasopressin response through the V2R...
  68. ncbi Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal degradation pathway
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital East, 149 13th St, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 288:C1390-401. 2005
    ..This degradative pathway may be an adaptive response to allow receptor-ligand association in the hypertonic and acidic environment of the renal medulla...
  69. pmc Angiotensin II and hypertonicity modulate proximal tubular aquaporin 1 expression
    Richard Bouley
    Center for Systems Biology, Program in Membrane Biology, Nephrology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 297:F1575-86. 2009
    ....
  70. pmc Regulation of the V-ATPase in kidney epithelial cells: dual role in acid-base homeostasis and vesicle trafficking
    Dennis Brown
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, MA 02114, USA
    J Exp Biol 212:1762-72. 2009
    ..Recent information about these pH-sensing mechanisms, which include the role of the V-ATPase itself as a pH sensor and the soluble adenylyl cyclase as a bicarbonate sensor, will be addressed in this review...
  71. pmc Vasopressin-induced differential stimulation of AQP4 splice variants regulates the in-membrane assembly of orthogonal arrays
    Alfred N Van Hoek
    Simches Research Center, 8 208, 185 Cambridge St, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 296:F1396-404. 2009
    ....
  72. ncbi Functional role of the NPxxY motif in internalization of the type 2 vasopressin receptor in LLC-PK1 cells
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Am J Physiol Cell Physiol 285:C750-62. 2003
    ..However, this motif is not essential for the apical/basolateral sorting and polarized distribution of the V2R in LLC-PK1a cells or for adenylyl cyclase-mediated signal transduction...
  73. ncbi Detection of ClC-3 and ClC-5 in epididymal epithelium: immunofluorescence and RT-PCR after LCM
    Corinne Isnard-Bagnis
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 284:C220-32. 2003
    ..ClC-5 might facilitate endosome trafficking in the epididymis, as has been proposed in the kidney...
  74. ncbi Expression of the 56-kDa B2 subunit isoform of the vacuolar H(+)-ATPase in proton-secreting cells of the kidney and epididymis
    Teodor G Paunescu
    Program in Membrane Biology Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 287:C149-62. 2004
    ..These findings indicate that in addition to its role in the acidification of intracellular organelles, the B2 isoform could also contribute to transepithelial proton secretion and the maintenance of acid-base homeostasis...
  75. ncbi Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens
    Nuria Pastor-Soler
    Renal Unit and Program in Membrane Biology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Biol Reprod 66:1716-22. 2002
    ..These data indicate that the expression of AQP9, a likely candidate for apical transepithelial fluid and solute transport in several regions of the male reproductive tract, is modulated by androgens in the adult rat epididymis...
  76. pmc Sensing, signaling and sorting events in kidney epithelial cell physiology
    Dennis Brown
    Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Traffic 10:275-84. 2009
    ....
  77. pmc Impaired tubular uptake explains albuminuria in early diabetic nephropathy
    Leileata M Russo
    Program in Membrane Biology, Division of Nephrology, Massachusetts General Hospital Harvard Medical School, Simches Research Center, Boston, MA 02114, USA
    J Am Soc Nephrol 20:489-94. 2009
    ..In conclusion, in the early stages of diabetic nephropathy, the PT plays a major role in the development of albuminuria, which may be preceded by peptideuria...
  78. pmc Commensal-associated molecular patterns induce selective toll-like receptor-trafficking from apical membrane to cytoplasmic compartments in polarized intestinal epithelium
    Elke Cario
    Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Am J Pathol 160:165-73. 2002
    ..The results of this dynamic epithelial surveillance can then be conveyed to the underlying cell populations of the lamina propria via these innate immune pattern recognition receptors...
  79. pmc RGS4 and RGS2 bind coatomer and inhibit COPI association with Golgi membranes and intracellular transport
    B M Sullivan
    Renal Unit, Program in Membrane Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Mol Biol Cell 11:3155-68. 2000
    ..Thus, these data support the hypothesis that these RGS proteins sequester coatomer in the cytoplasm and inhibit its recruitment onto Golgi membranes, which may in turn modulate Golgi-plasma membrane or intra-Golgi transport...
  80. ncbi Membrane localization and topology of leukotriene C4 synthase
    Peter Christmas
    Renal Unit and Program in Membrane Biology, Department of Medicine, Harvard Medical School and Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA
    J Biol Chem 277:28902-8. 2002
    ..The differential localization of two very similar integral membrane proteins suggests that mechanisms other than size-dependent exclusion regulate their passage to the inner nuclear membrane...
  81. ncbi Role of NHERF1, cystic fibrosis transmembrane conductance regulator, and cAMP in the regulation of aquaporin 9
    Christine Pietrement
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Simches Research Center, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA
    J Biol Chem 283:2986-96. 2008
    ..We propose that CFTR is an important regulator of AQP9 and that the interaction between AQP9, NHERF1, and CFTR may facilitate the activation of AQP9 by cAMP...
  82. ncbi Nephrin dissociates from actin, and its expression is reduced in early experimental membranous nephropathy
    Huaiping Yuan
    Renal Section and Department of Medicine, Evans Biomedical Research Center, Boston University Medical Center, 650 Albany Street, Boston, MA 02118, USA
    J Am Soc Nephrol 13:946-56. 2002
    ..These events may underlie the loss of podocyte barrier function in membranous nephropathy...
  83. pmc Deletion of Cavin/PTRF causes global loss of caveolae, dyslipidemia, and glucose intolerance
    Libin Liu
    Department of Biochemistry, Boston University School of Medicine, 715 Albany Street, Boston, MA 02118, USA
    Cell Metab 8:310-7. 2008
    ..Our results underscore the multiorgan role of caveolae in metabolic regulation and the obligate presence of Cavin for caveolae formation...
  84. pmc Proteomic analysis of V-ATPase-rich cells harvested from the kidney and epididymis by fluorescence-activated cell sorting
    Nicolas Da Silva
    Harvard Medical School, Boston, MA, USA
    Am J Physiol Cell Physiol 298:C1326-42. 2010
    ..These proteomic databases provide a framework for comprehensive future analysis of the common and distinct functions of V-ATPase-B1-expressing cells in the kidney and epididymis...
  85. ncbi Organ distribution in rats of two members of the low-density lipoprotein receptor gene family, gp330 and LRP/alpha 2MR, and the receptor-associated protein (RAP)
    G Zheng
    Department of Pathology, Massachusetts General Hospital, Boston
    J Histochem Cytochem 42:531-42. 1994
    ..Since RAP is found largely within cells, its major physiological function may be concerned with intracellular assembly or trafficking of the receptors to which it binds...
  86. pmc Expression of the mouse Na-K-2Cl cotransporter, mBSC2, in the terminal inner medullary collecting duct, the glomerular and extraglomerular mesangium, and the glomerular afferent arteriole
    M R Kaplan
    Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts, USA
    J Clin Invest 98:723-30. 1996
    ....
  87. ncbi Aberrant 11beta-hydroxysteroid dehydrogenase-1 activity in the cpk mouse: implications for regulation by the Ke 6 gene
    N Aziz
    Department of Medicine, Children s Hospital, Boston, Massachusetts 02115, USA
    Endocrinology 137:5581-8. 1996
    ..These results suggest that down-regulation of the Ke 6 gene may lead to elevated corticosterone levels, mediated through an inhibition of 11betaHSD-1 activity...
  88. pmc Inhibitory effects of omacetaxine on leukemic stem cells and BCR-ABL-induced chronic myeloid leukemia and acute lymphoblastic leukemia in mice
    Y Chen
    Division of Hematology Oncology, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Leukemia 23:1446-54. 2009
    ..Treatment with omacetaxine decreased the number of leukemia stem cells and prolonged the survival of mice with BCR-ABL-induced CML or B-ALL...
  89. ncbi The actin-binding proteins adseverin and gelsolin are both highly expressed but differentially localized in kidney and intestine
    A Lueck
    Brigham and Women s Hospital, Harvard Medical School, Department of Medicine, Division of Experimental Medicine, Boston, MA 02115, USA
    J Cell Sci 111:3633-43. 1998
    ..The observations indicate high level expression of adseverin in specific cells of the kidney and colon, and suggest a previously unrecognized function of adseverin in epithelial cell function...
  90. pmc Aquaporin 9 expression in the developing rat epididymis is modulated by steroid hormones
    NURIA M PASTOR-SOLER
    Program in Membrane Biology and Nephrology Division, Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Reproduction 139:613-21. 2010
    ..Our data show that expression of AQP9 in the developing rat epididymis is downregulated by neonatal DES, GNRHa, EE, and flutamide, and that the effects mediated by estrogens can be prevented by testosterone administration...
  91. ncbi New insights into the regulation of V-ATPase-dependent proton secretion
    Sylvie Breton
    Program in Membrane Biology, Nephrology Division, Massachusetts General Hospital, and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114 2790, USA
    Am J Physiol Renal Physiol 292:F1-10. 2007
    ..Modulation of the V-ATPase-rich cell phenotype and the pathophysiology of the V-ATPase in humans and experimental animals are also discussed...
  92. doi Renal outcomes with different fixed-dose combination therapies in patients with hypertension at high risk for cardiovascular events (ACCOMPLISH): a prespecified secondary analysis of a randomised controlled trial
    George L Bakris
    Hypertensive Diseases Unit, Department of Medicine, University of Chicago Pritzker School of Medicine, Chicago, IL 60637, USA
    Lancet 375:1173-81. 2010
    ..We assessed the effects of these drug combinations on progression of chronic kidney disease...
  93. doi Low-level persistence of human papillomavirus 16 DNA in a cohort of closely followed adolescent women
    B Weaver
    Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA
    J Med Virol 83:1362-9. 2011
    ..5 days. In adolescent females, duration of HPV 16 detection was significantly longer when TSN-PCR-16 was combined with LA-HPV. Some apparently cleared HPV 16 could be shown to persist at low levels using nested PCR...
  94. ncbi Physiological importance of endosomal acidification: potential role in proximal tubulopathies
    Vladimir Marshansky
    Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Boston 02129 2020, USA
    Curr Opin Nephrol Hypertens 11:527-37. 2002
    ....
  95. pmc Dragon enhances BMP signaling and increases transepithelial resistance in kidney epithelial cells
    Yin Xia
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Am Soc Nephrol 21:666-77. 2010
    ..Taken together, these results suggest that Dragon may enhance BMP signaling in renal tubular epithelial cells and maintain normal renal physiology...
  96. pmc Transepithelial projections from basal cells are luminal sensors in pseudostratified epithelia
    Winnie Wai Chi Shum
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, MA 02114, USA
    Cell 135:1108-17. 2008
    ..We propose a paradigm in which basal cells scan and sense the luminal environment of pseudostratified epithelia and modulate epithelial function by a mechanism involving crosstalk with other epithelial cells...
  97. pmc TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function
    Jochen Reiser
    Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02129, USA
    Nat Genet 37:739-44. 2005
    ..Two of the TRPC6 mutants had increased current amplitudes. These data show that TRPC6 channel activity at the slit diaphragm is essential for proper regulation of podocyte structure and function...
  98. ncbi Segmental and cellular expression of aquaporins in the male excurrent duct
    Nicolas Da Silva
    Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02114 2790, USA
    Biochim Biophys Acta 1758:1025-33. 2006
    ..The present article reviews our current knowledge regarding the distribution and function of aquaporins in the male excurrent duct...
  99. ncbi Cryptdin gene expression in developing mouse small intestine
    D Darmoul
    Gastrointestinal Unit, Massachusetts General Hospital, Boston, USA
    Am J Physiol 272:G197-206. 1997
    ....
  100. ncbi Identification of cytoskeletal regulatory proteins required for efficient phagocytosis in Drosophila
    Alan M Pearson
    Department of Pediatrics, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Microbes Infect 5:815-24. 2003
    ..This study demonstrates that Drosophila provides a viable model system in which to dissect the complex interactions that regulate phagocytosis...
  101. ncbi In vivo molecular probing of cellular compartments with gold nanoparticles and nanoaggregates
    Janina Kneipp
    Wellman Center for Photomedicine, Harvard University, Medical School, Boston, Massachusetts 02114, USA
    Nano Lett 6:2225-31. 2006
    ..The results have implications for medical and biotechnology applications of SERS nanosensors in cells...

Research Grants21

  1. An MMi CellCut Laser Mircrodissection System
    Dennis Brown; Fiscal Year: 2006
    ..Providing laser microdissection access and expertise to the MGH research community will be an important part of our mission and bridges the gap between pure microscopy studies and functional genomics that take advantage ..
  2. CELLULAR BIOLOGY OF RENAL FUNCTION AND DISEASE
    Dennis Brown; Fiscal Year: 2007
    ....
  3. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2005
    ..abstract_text> ..
  4. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2002
    ..abstract_text> ..
  5. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 1993
    ..An important and unique aspect of the proposed studies is that they will mostly be carried out in situ using markers of endogenous proteins that are of functional importance in kidney physiology...
  6. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 1991
    ..An important and unique aspect of the proposed studies is that they will mostly be carried out in situ using markers of endogenous proteins that are of functional importance in kidney physiology...
  7. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2006
    ..abstract_text> ..
  8. JEOL JEM-1011 Transmission Electron Microscope
    Dennis Brown; Fiscal Year: 2005
    ..abstract_text> ..
  9. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2004
    ..abstract_text> ..
  10. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2003
    ..abstract_text> ..
  11. Ultraview LCI Spinning Disk Confocal Microscope System
    Dennis Brown; Fiscal Year: 2003
    ..Thus, a wide-range of projects and investigators will benefit from this equipment, which will be integrated into a Core facility that has been operational for over 15 years. ..
  12. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2001
    ....
  13. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 2000
    ....
  14. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 1999
    ....
  15. CELLULAR REGULATION OF KIDNEY EPITHELIAL CELL POLARITY
    Dennis Brown; Fiscal Year: 1992
    ..An important and unique aspect of the proposed studies is that they will mostly be carried out in situ using markers of endogenous proteins that are of functional importance in kidney physiology...