S Breton

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. ncbi request reprint Postnatal development of H+ ATPase (proton-pump)-rich cells in rat epididymis
    S Breton
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital East, Charlestown 02129, USA
    Histochem Cell Biol 111:97-105. 1999
  2. ncbi request reprint The cellular physiology of carbonic anhydrases
    S Breton
    Program in Membrane Biology Renal Unit, Massachusetts General Hospital Charlestown, MA 02129, USA
    JOP 2:159-64. 2001
  3. ncbi request reprint The B1 subunit of the H+ATPase is a PDZ domain-binding protein. Colocalization with NHE-RF in renal B-intercalated cells
    S Breton
    Renal Unit and Program in Membrane Biology and the Molecular Neurogenetics Unit, Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA
    J Biol Chem 275:18219-24. 2000
  4. ncbi request reprint Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract
    S Breton
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Charlestown 02129, USA
    Am J Physiol Renal Physiol 278:F717-25. 2000
  5. ncbi request reprint H(+)V-ATPase-dependent luminal acidification in the kidney collecting duct and the epididymis/vas deferens: vesicle recycling and transcytotic pathways
    D Brown
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Boston, MA 02129, USA
    J Exp Biol 203:137-45. 2000
  6. ncbi request reprint Acidification of the male reproductive tract by a proton pumping (H+)-ATPase
    S Breton
    Renal Unit, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    Nat Med 2:470-2. 1996
  7. ncbi request reprint Na+/H+-exchange activity and immunolocalization of NHE3 in rat epididymis
    C Bagnis
    Program in Membrane Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Am J Physiol Renal Physiol 280:F426-36. 2001
  8. ncbi request reprint Immunolocalization of AE2 anion exchanger in rat and mouse epididymis
    L J Jensen
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Biol Reprod 61:973-80. 1999
  9. ncbi request reprint V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice
    R Lance Miller
    Dept of Pediatrics, Univ of Utah, 30 N 1900 East, Salt Lake City, UT 84132 2204, USA
    Am J Physiol Cell Physiol 288:C1134-44. 2005
  10. pmc The B1-subunit of the H(+) ATPase is required for maximal urinary acidification
    Karin E Finberg
    Departments of Genetics, Cellular and Molecular Physiology, Surgery, and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 102:13616-21. 2005

Collaborators

Detail Information

Publications35

  1. ncbi request reprint Postnatal development of H+ ATPase (proton-pump)-rich cells in rat epididymis
    S Breton
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital East, Charlestown 02129, USA
    Histochem Cell Biol 111:97-105. 1999
    ..These results demonstrate that differentiated subpopulations of proton-secreting epithelial cells appear early during epididymal development, and that the induction of H+ ATPase in these cells occurs prior to sexual maturation...
  2. ncbi request reprint The cellular physiology of carbonic anhydrases
    S Breton
    Program in Membrane Biology Renal Unit, Massachusetts General Hospital Charlestown, MA 02129, USA
    JOP 2:159-64. 2001
    ..The localization of these isoforms in selected epithelia that are involved in net acid/base transport, such as kidney proximal tubules and collecting ducts, and tubules from the male reproductive tract will be reviewed...
  3. ncbi request reprint The B1 subunit of the H+ATPase is a PDZ domain-binding protein. Colocalization with NHE-RF in renal B-intercalated cells
    S Breton
    Renal Unit and Program in Membrane Biology and the Molecular Neurogenetics Unit, Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA
    J Biol Chem 275:18219-24. 2000
    ..The colocalization of NHE-RF and H(+)ATPase in B- but not A-intercalated cells suggests a role in generating, maintaining, or modulating the variable H(+)ATPase polarity that characterizes the B-cell phenotype...
  4. ncbi request reprint Tetanus toxin-mediated cleavage of cellubrevin inhibits proton secretion in the male reproductive tract
    S Breton
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Charlestown 02129, USA
    Am J Physiol Renal Physiol 278:F717-25. 2000
    ..These results suggest that H(+)-ATPase is actively endocytosed and exocytosed in proton-secreting cells of the epididymis and vas deferens and that net proton secretion requires the participation of the v-SNARE cellubrevin...
  5. ncbi request reprint H(+)V-ATPase-dependent luminal acidification in the kidney collecting duct and the epididymis/vas deferens: vesicle recycling and transcytotic pathways
    D Brown
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Department of Pathology, Harvard Medical School, Boston, MA 02129, USA
    J Exp Biol 203:137-45. 2000
    ..We propose that the 'polarity reversal' of the H(+)V-ATPase in B-intercalated cells is mediated by a physiologically regulated transcytotic pathway that may be similar to that existing in some other cell types...
  6. ncbi request reprint Acidification of the male reproductive tract by a proton pumping (H+)-ATPase
    S Breton
    Renal Unit, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    Nat Med 2:470-2. 1996
    ..This acidification mechanism is a potential target for novel strategies aimed at modulating the acidification capacity of parts of the male reproductive tract and, therefore, in regulating male fertility...
  7. ncbi request reprint Na+/H+-exchange activity and immunolocalization of NHE3 in rat epididymis
    C Bagnis
    Program in Membrane Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Am J Physiol Renal Physiol 280:F426-36. 2001
    ..These data indicate that HOE694- and EIPA-sensitive Na+/H+ exchange may participate, together with the H+-ATPase, in luminal acidification in the male excurrent duct...
  8. ncbi request reprint Immunolocalization of AE2 anion exchanger in rat and mouse epididymis
    L J Jensen
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Biol Reprod 61:973-80. 1999
    ..Basolateral AE2 may participate in bicarbonate reabsorption and luminal acidification, and/or may be involved in intracellular pH homeostasis of epithelial cells of the male reproductive tract...
  9. ncbi request reprint V-ATPase B1-subunit promoter drives expression of EGFP in intercalated cells of kidney, clear cells of epididymis and airway cells of lung in transgenic mice
    R Lance Miller
    Dept of Pediatrics, Univ of Utah, 30 N 1900 East, Salt Lake City, UT 84132 2204, USA
    Am J Physiol Cell Physiol 288:C1134-44. 2005
    ....
  10. pmc The B1-subunit of the H(+) ATPase is required for maximal urinary acidification
    Karin E Finberg
    Departments of Genetics, Cellular and Molecular Physiology, Surgery, and Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510, USA
    Proc Natl Acad Sci U S A 102:13616-21. 2005
    ....
  11. ncbi request reprint Postnatal expression of aquaporins in epithelial cells of the rat epididymis
    Nicolas Da Silva
    Massachusetts General Hospital, Program in Membrane Biology Nephrology Division, Boston, Massachusetts 02114, USA
    Biol Reprod 74:427-38. 2006
    ..AQPs may be involved not only in the transepithelial transport of water in the epididymis but also in the postnatal development of this organ, as suggested by the differential expression of AQP2...
  12. ncbi request reprint Pendrin regulation in mouse kidney primarily is chloride-dependent
    Marion Vallet
    INSERM U652, IFR58, Institut des Cordeliers, Paris, France
    J Am Soc Nephrol 17:2153-63. 2006
    ..These results suggest that factors that are associated with changes in distal chloride delivery govern pendrin expression in the connecting tubule and cortical collecting duct...
  13. ncbi request reprint New insights into the regulation of V-ATPase-dependent proton secretion
    Sylvie Breton
    Program in Membrane Biology, Nephrology Division, Massachusetts General Hospital, and Department of Medicine, Harvard Medical School, Boston, Massachusetts 02114 2790, USA
    Am J Physiol Renal Physiol 292:F1-10. 2007
    ..Modulation of the V-ATPase-rich cell phenotype and the pathophysiology of the V-ATPase in humans and experimental animals are also discussed...
  14. ncbi request reprint Relocalization of the V-ATPase B2 subunit to the apical membrane of epididymal clear cells of mice deficient in the B1 subunit
    Nicolas Da Silva
    Massachusetts General Hospital, Harvard Medical School, Program in Membrane Biology, Nephrology Div, 185 Cambridge St, CPZN 8150, Boston, MA 02114 2790, USA
    Am J Physiol Cell Physiol 293:C199-210. 2007
    ..This relocation compensates for the absence of functional B1 and maintains the luminal pH in an acidic range that is compatible with fertility...
  15. ncbi request reprint Compensatory membrane expression of the V-ATPase B2 subunit isoform in renal medullary intercalated cells of B1-deficient mice
    Teodor G Paunescu
    Div of Nephrology, Massachusetts General Hospital, 185 Cambridge St, CPZN 8150, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 293:F1915-26. 2007
    ..However, our results show no increase in cell surface V-ATPase activity in response to metabolic acidosis in ICs from these animals, consistent with their inability to appropriately acidify their urine under these conditions...
  16. ncbi request reprint Association of soluble adenylyl cyclase with the V-ATPase in renal epithelial cells
    Teodor G Paunescu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 294:F130-8. 2008
    ..This suggests that these enzymes have a close association and could be part of a protein complex that is involved in regulating renal distal proton secretion...
  17. ncbi request reprint Role of NHERF1, cystic fibrosis transmembrane conductance regulator, and cAMP in the regulation of aquaporin 9
    Christine Pietrement
    Center for Systems Biology, Program in Membrane Biology Nephrology Division, Simches Research Center, Massachusetts General Hospital, 185 Cambridge Street, Boston, MA 02114, USA
    J Biol Chem 283:2986-96. 2008
    ..We propose that CFTR is an important regulator of AQP9 and that the interaction between AQP9, NHERF1, and CFTR may facilitate the activation of AQP9 by cAMP...
  18. ncbi request reprint Modulation of the actin cytoskeleton via gelsolin regulates vacuolar H+-ATPase recycling
    Valerie Beaulieu
    Program in Membrane Biology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    J Biol Chem 280:8452-63. 2005
    ..Therefore, modulation of the actin cortex by this severing and capping protein may represent a common mechanism by which these cells regulate their rate of proton secretion...
  19. ncbi request reprint Increased luminal pH in the epididymis of infertile c-ros knockout mice and the expression of sodium-hydrogen exchangers and vacuolar proton pump H+-ATPase
    Ching Hei Yeung
    Institute of Reproductive Medicine, University of Munster, Domagkstrasse 11, D 48129 Munster, Germany
    Mol Reprod Dev 68:159-68. 2004
    ..The increase in luminal fluid pH in the KO mice could also be contributed to by other epithelial regulating factors including the Na(+)-dependent glutamate transporter EAAC1 formerly reported to be down regulated in the KO...
  20. ncbi request reprint Alkaline pH- and cAMP-induced V-ATPase membrane accumulation is mediated by protein kinase A in epididymal clear cells
    NURIA M PASTOR-SOLER
    Renal Electrolyte Division, Department of Medicine, University of Pittsburgh School of Medicine, A915 Scaife Hall, 3550 Terrace St, Pittsburgh, PA 15263, USA
    Am J Physiol Cell Physiol 294:C488-94. 2008
    ..We conclude that direct stimulation of PKA activity by cAMP is necessary and sufficient for the alkaline pH-induced accumulation of V-ATPase in clear cell apical microvilli...
  21. ncbi request reprint Detection of ClC-3 and ClC-5 in epididymal epithelium: immunofluorescence and RT-PCR after LCM
    Corinne Isnard-Bagnis
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital, 149 13th Street, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 284:C220-32. 2003
    ..ClC-5 might facilitate endosome trafficking in the epididymis, as has been proposed in the kidney...
  22. ncbi request reprint Modulation of the onset of postnatal development of H(+)-ATPase-rich cells by steroid hormones in rat epididymis
    Jane S Fisher
    Medical Research Council, Human Reproductive Sciences Unit, Edinburgh EH16 4SB, United Kingdom
    Biol Reprod 67:1106-14. 2002
    ....
  23. ncbi request reprint A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse
    Carsten A Wagner
    Institute of Physiology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland
    Pflugers Arch 446:623-32. 2003
    ..This technique will facilitate further characterization of kidney function in normal and genetically manipulated animals...
  24. pmc Bicarbonate-regulated adenylyl cyclase (sAC) is a sensor that regulates pH-dependent V-ATPase recycling
    Nuria Pastor-Soler
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    J Biol Chem 278:49523-9. 2003
    ..As sAC is expressed in other acid/base transporting epithelia, including kidney and choroid plexus, this cAMP-dependent signal transduction pathway may be a widespread mechanism that allows cells to sense and modulate extracellular pH...
  25. ncbi request reprint Expression of aquaporin 9 in the adult rat epididymal epithelium is modulated by androgens
    Nuria Pastor-Soler
    Renal Unit and Program in Membrane Biology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Biol Reprod 66:1716-22. 2002
    ..These data indicate that the expression of AQP9, a likely candidate for apical transepithelial fluid and solute transport in several regions of the male reproductive tract, is modulated by androgens in the adult rat epididymis...
  26. ncbi request reprint Expression of the 56-kDa B2 subunit isoform of the vacuolar H(+)-ATPase in proton-secreting cells of the kidney and epididymis
    Teodor G Paunescu
    Program in Membrane Biology Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 287:C149-62. 2004
    ..These findings indicate that in addition to its role in the acidification of intracellular organelles, the B2 isoform could also contribute to transepithelial proton secretion and the maintenance of acid-base homeostasis...
  27. ncbi request reprint Renal principal cell-specific expression of green fluorescent protein in transgenic mice
    Ludmilla Zharkikh
    Department of Pediatrics, University of Utah, Salt Lake City, Utah 84132, USA
    Am J Physiol Renal Physiol 283:F1351-64. 2002
    ..This transgenic mouse model can be used in future studies of gene expression during the development, differentiation, and maturation of renal principal cells...
  28. ncbi request reprint Antigen retrieval reveals widespread basolateral expression of syntaxin 3 in renal epithelia
    Sylvie Breton
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 282:F523-9. 2002
    ..The exclusive basolateral location of syntaxin 3 in situ, however, contrasts with the apical location of this SNARE protein in some kidney epithelial cells in culture...
  29. ncbi request reprint Regulation of vacuolar proton pumping ATPase-dependent luminal acidification in the epididymis
    Nicolas Da Silva
    Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Boston, MA 02114, USA
    Asian J Androl 9:476-82. 2007
    ..In the present paper, selected aspects of V-ATPase regulation in clear cells are described and potential pathologies associated with mutations of some of the V-ATPase subunits are discussed...
  30. ncbi request reprint Hypertonicity is involved in redirecting the aquaporin-2 water channel into the basolateral, instead of the apical, plasma membrane of renal epithelial cells
    Bas W M van Balkom
    Department of Cell Physiology, Nijmegen Center for Molecular Life Sciences, UMC St Radboud, The Netherlands
    J Biol Chem 278:1101-7. 2003
    ..These data reveal for the first time that chronic hypertonicity can program cells in vitro and in vivo to change the insertion of a protein into the basolateral membrane instead of the apical membrane...
  31. ncbi request reprint Segmental and cellular expression of aquaporins in the male excurrent duct
    Nicolas Da Silva
    Program in Membrane Biology Nephrology Division, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, Boston, MA 02114 2790, USA
    Biochim Biophys Acta 1758:1025-33. 2006
    ..The present article reviews our current knowledge regarding the distribution and function of aquaporins in the male excurrent duct...
  32. ncbi request reprint Loss of basolateral invaginations in proximal tubules of cadmium-intoxicated rats is independent of microtubules and clathrin
    Ivan Sabolic
    Unit of Molecular Toxicology, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, P O Box 291, HR 10001 Zagreb, Croatia
    Toxicology 218:149-63. 2006
    ..This loss is accompanied by, but it is not dependent on perturbation of microtubule organization and loss of membrane-associated clathrin...
  33. ncbi request reprint Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra)
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Rm 8207, Massachusetts General Hospital East, 149 13th St Charlestown, MA 02129, USA
    Am J Physiol Renal Physiol 288:F1103-12. 2005
    ....
  34. ncbi request reprint Differential expression and targeting of endogenous Arf1 and Arf6 small GTPases in kidney epithelial cells in situ
    Jaafar El-Annan
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Boston, MA 02129 2020, USA
    Am J Physiol Cell Physiol 286:C768-78. 2004
    ..The results provide a framework on which to base and interpret future studies on the role of Arf GTPases in the multitude of cellular trafficking events that occur in renal tubular epithelial cells...
  35. ncbi request reprint Renal vacuolar H+-ATPase
    Carsten A Wagner
    Institute of Physiology, Univ of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Physiol Rev 84:1263-314. 2004
    ..This regulation is at least in part mediated by various hormones and protein-protein interactions between regulatory proteins and multiple subunits of the pump...