Research Topics
| XANDRA OWENS BREAKEFIELDSummaryAffiliation: Harvard University Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Neural precursor cells and their role in neuro-oncologyKhalid Shah
Department of Neurology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA, USA
Dev Neurosci 26:118-30. 2004..Examples are provided of monitoring migration of NPCs by bioluminescence imaging in living animals and of using them to deliver the apoptotic protein, TRAIL, to kill tumor cells...
RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controlsMikkel Noerholm
Department of Neurology, Neurosurgery and Radiology, Massachusetts General Hospital and Program in Neuroscience, Harvard Medical School, Boston, MA 02114, USA
BMC Cancer 12:22. 2012..RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients...- Healing genes in the nervous systemXandra O Breakefield
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Neuron 68:178-81. 2010.... 
The pathophysiological basis of dystoniasXandra O Breakefield
Department of Neurology and Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
Nat Rev Neurosci 9:222-34. 2008....- HSV-1 virions engineered for specific binding to cell surface receptorsPaola Grandi
Department of Neurology and Department of Radiology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, MA 02129, USA
Mol Ther 9:419-27. 2004.... - Molecular cloning and expression of rat torsinA in the normal and genetically dystonic (dt) ratPhilipp Ziefer
Molecular Neurogenetics Unit, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Brain Res Mol Brain Res 101:132-5. 2002..5 kb. In situ hybridization reveals a widespread distribution of torsinA mRNA within brain. No mutations were identified in the coding region of the gene in the genetically dystonic (dt) rat... - Mutant torsinA interferes with protein processing through the secretory pathway in DYT1 dystonia cellsJeffrey W Hewett
Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, and Program in Neuroscience, Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 104:7271-6. 2007..These studies demonstrate the exquisite sensitivity of this reporter system for quantitation of processing through the secretory pathway and support a role for torsinA as an ER chaperone protein... - Intravascular delivery of neural stem cell lines to target intracranial and extracranial tumors of neural and non-neural originAlice B Brown
Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USA
Hum Gene Ther 14:1777-85. 2003.... - Dynamics of transgene expression in human glioblastoma cells mediated by herpes simplex virus/adeno-associated virus amplicon vectorsPaula Lam
Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Boston, MA 02129, USA
Hum Gene Ther 13:2147-59. 2002..Taken together, these data support employing AAV ITRs, in the context of HSV-1 amplicon vectors, to enhance short-term levels of transgene expression... - Dopamine release is impaired in a mouse model of DYT1 dystoniaAygul Balcioglu
MassGeneral Institute for Neurodegenerative Disease, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, USA
J Neurochem 102:783-8. 2007..The defect in DA release as observed may contribute to the abnormalities in motor learning as previously documented in this transgenic mouse model, and may contribute to the clinical symptoms of the human disorder... - TorsinA and early-onset torsion dystoniaD Cristopher Bragg
Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, USA
Adv Neurol 94:87-93. 2004 - TorsinA in PC12 cells: localization in the endoplasmic reticulum and response to stressJeffrey Hewett
Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, Massachusetts, USA
J Neurosci Res 72:158-68. 2003..Mutant torsinA may interfere with and/or compromise ER functions, especially in dopaminergic neurons, which have high levels of torsinA and are intrinsically vulnerable to oxidative stress... - Herpes simplex virus/adeno-associated virus hybrid vectors for gene transfer to neurons. Preparation and useLauren C Costantini
Neuroregeneration Laboratory, Harvard Medical School, McLean Hospital, Belmont, MA, USA
Methods Mol Med 69:461-79. 2002 - Effects of genetic variations in the dystonia protein torsinA: identification of polymorphism at residue 216 as protein modifierNorman Kock
Department of Neurology, Massachusetts General Hospital, Boston, MA 02114, USA
Hum Mol Genet 15:1355-64. 2006..They also suggest possible connections between the allelic polymorphism at residue 216 and the penetrance of DYT1 dystonia, as well as a possible role for this polymorphism in related disease states... - TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeletonFlavia C Nery
Molecular Neurogenetics Unit, Department of Neurology and Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital and Program in Neuroscience, Harvard Medical School, Boston, MA 02114, USA
J Cell Sci 121:3476-86. 2008.... - Potentiated gene delivery to tumors using herpes simplex virus/Epstein-Barr virus/RV tribrid amplicon vectorsJürgen A Hampl
Molecular Neurogenetics Unit, Department of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
Hum Gene Ther 14:611-26. 2003..The application of this system, which can integrate a transgene cassette into tumors with therapeutic bystander effects, could increase the local amplification effect to a level of clinical relevance... - The early onset dystonia protein torsinA interacts with kinesin light chain 1Christoph Kamm
Molecular Neurogenetics Unit, Departments of Neurology and Radiology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02114, USA
J Biol Chem 279:19882-92. 2004..These studies suggest that wild-type torsinA undergoes anterograde transport along microtubules mediated by kinesin and may act as a molecular chaperone regulating kinesin activity and/or cargo binding... - Targeting HSV amplicon vectorsPaola Grandi
Departments of Neurology and Radiology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Charlestown, MA 02129, USA
Methods 33:179-86. 2004..Retargeted vectors can provide an additional tool for increasing the efficiency of gene delivery to specific cell types... - TorsinB--perinuclear location and association with torsinAJeffrey W Hewett
Departments of Neurology and Radiology, Molecular Neurogenetics Unit, Massachusetts General Hospital, 13th Street, Charlestown, MA 02129, USA
J Neurochem 89:1186-94. 2004..We conclude that torsinB and torsinA are localized in overlapping cell compartments within the same protein complex, and thus may carry out related functions in vivo... - Targeted integration of functional human ATM cDNA into genome mediated by HSV/AAV hybrid amplicon vectorMaria L Cortes
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
Mol Ther 16:81-8. 2008.... - Improved HSV-1 amplicon packaging system using ICP27-deleted, oversized HSV-1 BAC DNAYoshinaga Saeki
Molecular Neuro-Oncology Lab, Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA
Methods Mol Med 76:51-60. 2003 - Modified herpes simplex virus delivery of enhanced GFP into the central nervous systemVladislav M Sandler
HHMI, Children's Hospital, Boston, MA, USA
J Neurosci Methods 121:211-9. 2002..We conclude that the HSV-1 amplicon is a valuable tool for gene delivery in the rat central nervous system... - siRNA knock-down of mutant torsinA restores processing through secretory pathway in DYT1 dystonia cellsJeffrey W Hewett
Molecular Neurogenetics Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
Hum Mol Genet 17:1436-45. 2008..The ability of allele-specific siRNA for torsinADeltaE to normalize secretory function in DYT1 patient cells supports its potential role as a therapeutic agent in early onset torsion dystonia... - In vivo imaging of S-TRAIL-mediated tumor regression and apoptosisKhalid Shah
Center for Molecular Imaging Research, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Mol Ther 11:926-31. 2005..The use of S-TRAIL as a therapeutic protein and the ability to image noninvasively both apoptosis and any other cellular events in real time have important clinical implications... - Glioma therapy and real-time imaging of neural precursor cell migration and tumor regressionKhalid Shah
Department of Neurology, Massachusetts General Hospital East, Harvard Medical School, 13th Street, Building 149, Charlestown, MA 02129, USA
Ann Neurol 57:34-41. 2005..These studies demonstrate the potential of NPCs as therapeutically effective delivery vehicles for the treatment of gliomas and also provide important tools to evaluate the migration of NPCs and changes in glioma burden in vivo... - Treatment of schwannomas with an oncolytic recombinant herpes simplex virus in murine models of neurofibromatosis type 2Shanta M Messerli
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, and Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
Hum Gene Ther 17:20-30. 2006.... - Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivoBakhos A Tannous
Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA
Mol Ther 11:435-43. 2005.... - Developmental patterns of torsinA and torsinB expressionAnju Vasudevan
Developmental Neurobiology, Massachusetts General Hospital, 13th Street, Building 149, 6th Floor, Charlestown, MA 02129, USA
Brain Res 1073:139-45. 2006.... - HSV amplicon vectors for cancer therapyKhalid Shah
Center for Molecular Imaging Research and Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, USA
Curr Gene Ther 6:361-70. 2006..Amplicon vectors have also proven to be a versatile tool to explore imaging modalities to monitor gene delivery and tumor responses to therapeutic intervention... - Preventing growth of brain tumors by creating a zone of resistanceCasey A Maguire
Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, Massachusetts 02129, USA
Mol Ther 16:1695-702. 2008..To our knowledge this is the first direct demonstration of the efficacy of targeting gene delivery exclusively to normal brain cells for brain tumor therapy... - A highly sensitive assay for monitoring the secretory pathway and ER stressChristian E Badr
Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, Boston, Massachusetts, United States of America Program in Neuroscience, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS ONE 2:e571. 2007..We have developed a highly sensitive assay to monitor processing of proteins through the secretory pathway and endoplasmic reticulum (ER) stress in real-time based on the naturally secreted Gaussia luciferase (Gluc)... - A secreted luciferase for ex vivo monitoring of in vivo processesThomas Wurdinger
Molecular Neurogenetics Unit, Department of Neurology, Harvard Medical School, 149 13th St, Charlestown, Massachusetts 02129, USA
Nat Methods 5:171-3. 2008.... - miR-296 regulates growth factor receptor overexpression in angiogenic endothelial cellsThomas Wurdinger
Department of Neurology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA
Cancer Cell 14:382-93. 2008..Furthermore, inhibition of miR-296 with antagomirs reduces angiogenesis in tumor xenografts in vivo... - Mutant sodium channel for tumor therapyBakhos A Tannous
Department of Neurology, Molecular Neurogenetics Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, USA
Mol Ther 17:810-9. 2009..The advantage of this direct mode of tumor therapy is that all types of tumor cells become susceptible and death is rapid with no time for the tumor cells to become resistant... - Herpes simplex virus type 1 amplicons and their hybrid virus partners, EBV, AAV, and retrovirusAngelika Oehmig
Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA
Curr Gene Ther 4:385-408. 2004.... - Inhibition of N-linked glycosylation prevents inclusion formation by the dystonia-related mutant form of torsinAD Cristopher Bragg
Departments of Neurology and Radiology Massachusetts General Hospital, Charlestown, MA 02129, USA
Mol Cell Neurosci 27:417-26. 2004..These results thus indicate that torsinADeltaE must achieve a specific conformation to induce formation of intracellular membrane inclusions... - Herpes simplex virus type 1 amplicon vector-mediated gene transfer to muscleYaming Wang
Department of Anesthesia, Brigham and Women s Hospital, Massachusetts General Hospital, Boston, MA 02115, USA
Hum Gene Ther 13:261-73. 2002..We conclude that the HSV-1 amplicon vector is a promising vehicle for gene delivery in DMD. However, new strategies need to be evaluated to increase the stability of transgene expression... - TorsinA protein and neuropathology in early onset generalized dystonia with GAG deletionKevin Rostasy
Pediatric Neurology Floating Hospital, Boston, MA, USA
Neurobiol Dis 12:11-24. 2003.... - Single HSV-amplicon vector mediates drug-induced gene expression via dimerizer systemSamuel Wang
Department of Neurology, and Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
Mol Ther 7:790-800. 2003..Gene induction in several neuronal models, including primary cell culture and organotypic cultures, as well as in rodent brain, was observed... - Distribution and ultrastructural localization of torsinA immunoreactivity in the human brainSarah J Augood
Neurology Service, Massachusetts General Hospital and Harvard Medical School, CNY 114 2300, 114 16th Street, Charlestown, MA 02129, USA
Brain Res 986:12-21. 2003.... - Dopamine transmission in DYT1 dystoniaSarah J Augood
Center for Aging, Genetics, and Neurodegeneration, Massachusetts General Hospital, Charlestown, Massachusetts, USA
Adv Neurol 94:53-60. 2004 - In vivo imaging of HIV protease activity in amplicon vector-transduced gliomasKhalid Shah
Center for Molecular Imaging Research, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, USA
Cancer Res 64:273-8. 2004..These findings may be directly applicable in using viral protease expression as a transgene marker in tumor therapy and may have implications in testing the efficacy of HIV-1PR inhibitors in vivo... - A heteroplasmic mitochondrial complex I gene mutation in adult-onset dystoniaDavid K Simon
Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, Boston, MA 02115, USA
Neurogenetics 4:199-205. 2003..01). This difference remains significant even after excluding the index patient (P=0.04). These data suggest that, among haplogroup H subjects, the presence of the A3796G mutation increases the risk of developing adult-onset dystonia... - Inducible release of TRAIL fusion proteins from a proapoptotic form for tumor therapyKhalid Shah
Molecular Neurogenetics Unit, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
Cancer Res 64:3236-42. 2004..This study serves as a template for design of recombinant proteins to enhance and control apoptosis of tumor cells via specific viral proteases and for use of viral proteases as in vivo reporters for cancer therapy... - Intrafamilial phenotypic variability of the DYT1 dystonia: from asymptomatic TOR1A gene carrier status to dystonic stormPuneet Opal
Department of Neurology, Baylor College of Medicine, Houston, Texas 77030, USA
Mov Disord 17:339-45. 2002..We conclude that marked phenotypic heterogeneity characterizes some families with DYT1 dystonia, suggesting a role for genetic, environmental, or other modifiers. These findings have implications for genetic testing and counseling... - Generation of stable retrovirus packaging cell lines after transduction with herpes simplex virus hybrid amplicon vectorsMiguel Sena-Esteves
Molecular Neurogenetics Unit and Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
J Gene Med 4:229-39. 2002..Conversion of cells with migratory or tumor/tissue homing properties could result in expansion of the spatial distribution or targeting capacity, respectively, of gene delivery by retrovirus vectors in vivo... - Impaired motor learning in mice expressing torsinA with the DYT1 dystonia mutationNutan Sharma
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
J Neurosci 25:5351-5. 2005..Together, these data suggest that these transgenic mice provide a useful model of the DeltaGAG carrier state that can be used to probe genetic and environmental factors that can trigger the dystonic state... - RNAi blocks DYT1 mutant torsinA inclusions in neuronsNorman Kock
Departments of Neurology and Radiology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, USA
Neurosci Lett 395:201-5. 2006..Vector-delivered siRNAs have the potential to decrease the adverse effects of this mutant protein in neurons without affecting wild-type protein... - Update on herpesvirus amplicon vectorsAngelika Oehmig
Department of Neurology, Massachusetts General Hospital, and Program in Neuroscience, Harvard Medical School, Boston, MA 02114, USA
Mol Ther 10:630-43. 2004 - Dystonia-causing mutant torsinA inhibits cell adhesion and neurite extension through interference with cytoskeletal dynamicsJeffrey W Hewett
Molecular Neurogenetics Unit, Departments of Neurology and Radiology, Massachusetts General Hospital, and Program in Neuroscience, Harvard Medical School, Boston, MA 02114, USA
Neurobiol Dis 22:98-111. 2006.... - Degradation of fibrillar collagen in a human melanoma xenograft improves the efficacy of an oncolytic herpes simplex virus vectorTrevor D McKee
Edwin L. Steele Laboratory, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachussetts 02114, USA
Cancer Res 66:2509-13. 2006..Thus, fibrillar collagen can be a formidable barrier to viral distribution and matrix-modifying treatments can significantly enhance the therapeutic response... - A novel method for imaging apoptosis using a caspase-1 near-infrared fluorescent probeShanta M Messerli
Departments of Neurology and Radiology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02115, USA
Neoplasia 6:95-105. 2004..This novel ICE-NIRF probe should prove useful in monitoring endogenous and vector-expressed caspase-1 activity, and potentially apoptosis in cell culture and in vivo... - Refined linkage to the RDP/DYT12 locus on 19q13.2 and evaluation of GRIK5 as a candidate geneChristoph Kamm
Molecular Neurogenetics Unit, Department of Neurology, Neuroscience Program, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
Mov Disord 19:845-7. 2004..9 cM interval flanked by markers D19S224 and D19S900. Evaluation of a positional candidate gene, the glutamate receptor subunit GRIK5, revealed no mutations... - Microfluidic isolation and transcriptome analysis of serum microvesiclesChihchen Chen
BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
Lab Chip 10:505-11. 2010..RNA of high quality can be extracted from these microvesicles providing a source of information about the genetic status of tumors to serve as biomarkers for diagnosis and prognosis of cancer... - Critical issues in gene therapy for neurologic diseaseGary Hsich
Molecular Neurogenetics Unit, Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, MA 02114, USA
Hum Gene Ther 13:579-604. 2002.... - FGF-2 regulates neurogenesis and degeneration in the dentate gyrus after traumatic brain injury in miceShinichi Yoshimura
Neuroscience Center, Radiology Department, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, Massachusetts, USA
J Clin Invest 112:1202-10. 2003..These results suggest that FGF-2 upregulates neurogenesis and protects neurons against degeneration in the adult hippocampus after TBI, and that FGF-2 supplementation via gene transfer can reduce GCL degeneration after TBI... - Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkersJohan Skog
Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, 149 13th Street, Charlestown, MA 02129, USA
Nat Cell Biol 10:1470-6. 2008..Thus, tumour-derived microvesicles may provide diagnostic information and aid in therapeutic decisions for cancer patients through a blood test... - Downregulated microRNA-200a in meningiomas promotes tumor growth by reducing E-cadherin and activating the Wnt/beta-catenin signaling pathwayOkay Saydam
Department of Neurology, Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston, Massachusetts 02129, USA
Mol Cell Biol 29:5923-40. 2009.... - Live visualization of herpes simplex virus type 1 compartment dynamicsAnna Paula de Oliveira
Institute of Virology, University of Zurich, Winterthurerstrasse 266a, CH 8057 Zurich, Switzerland
J Virol 82:4974-90. 2008..Similar strategies can also be applied to assess other dynamic events in the virus life cycle, such as entry and trafficking... - Epsilon-sarcoglycan mutations found in combination with other dystonia gene mutationsChristine Klein
Department of Neurology, Medical University of Lubeck, Lubeck, Germany
Ann Neurol 52:675-9. 2002..In both of the latter families, we also found a mutation of SGCE. The molecular mechanisms through which the detected mutations may contribute to myoclonus-dystonia remain to be determined... - HSV-1 amplicon peptide display vectorMatthew A Spear
Gene Therapy Program, Radiation Oncology, UCSD Cancer Center, UCSD Medical Center, University of California San Diego, MC 8757, 200 West Arbor Drive, La Jolla, CA, USA
J Virol Methods 107:71-9. 2003.... - Viral vectors for gene delivery to the nervous systemBeverly L Davidson
Program in Gene Therapy, Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
Nat Rev Neurosci 4:353-64. 2003 - TorsinA and heat shock proteins act as molecular chaperones: suppression of alpha-synuclein aggregationPamela J McLean
Alzheimer's Disease Research Unit, Center for Aging, Genetics and Neurodegeneration, Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA
J Neurochem 83:846-54. 2002..Moreover, these data support a role for chaperone proteins, including torsinA and heat shock proteins, in cellular responses to neurodegenerative inclusions... - Identification of a novel gene (HSN2) causing hereditary sensory and autonomic neuropathy type II through the Study of Canadian Genetic IsolatesRonald G Lafreniere
Xenon Genetics Research, Xenon Genetics Inc, Burnaby, British Columbia V5G 4W8, Canada
Am J Hum Genet 74:1064-73. 2004..The HSN2 protein may play a role in the development and/or maintenance of peripheral sensory neurons or their supporting Schwann cells... - Role of parkin mutations in 111 community-based patients with early-onset parkinsonismMartin Kann
Department of Neurology, Medical University of Lubeck, Lubeck, Germany
Ann Neurol 51:621-5. 2002..In summary, parkin mutations accounted for a low but significant percentage of early-onset parkinsonism patients in a community-derived sample... - TorsinA in the nuclear envelopeTeresa V Naismith
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA
Proc Natl Acad Sci U S A 101:7612-7. 2004..These results suggest that changes in interactions involving torsinA in the NE could be important for the pathogenesis of dystonia and point to torsinA and related proteins as a class of ATPases that may operate in the NE... - Metabolic biotinylation of cell surface receptors for in vivo imagingBakhos A Tannous
Center for Molecular Imaging Research, Massachusetts General Hospital-East, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA
Nat Methods 3:391-6. 2006..This BAP-TM allows noninvasive real-time imaging of any cell type transduced to express this reporter protein in culture or in vivo...
Research Grants
- IDENTIFICATION OF THE DYSTONIA GENEXandra Breakefield; Fiscal Year: 1993..Eventually this work will lead to identification of the dystonia gene and understanding of its function, which in turn should provide insight into the molecular etiology of this disease state and help in designing new therapies...
- IDENTIFICATION OF THE DYSTONIA GENEXandra Breakefield; Fiscal Year: 2001..These studies should provide us with insights into the possible function of dystonin in the basal ganglia. ..
- Tumor exosomes as agents of genetic changeXandra Breakefield; Fiscal Year: 2009..The priority score reflects the average of all the scores given by the full committee after a thorough discussion. ..
- Tumor exosomes as agents of genetic changeXANDRA OWENS BREAKEFIELD; Fiscal Year: 2010..abstract_text> ..
- Targeted Gene Therapy for Neurologic diseaseXandra Breakefield; Fiscal Year: 2005..abstract_text> ..
- Molecular Genetics of Inherited Neurological DiseasesXandra Breakefield; Fiscal Year: 2005..Collectively these studies provide a concerted effort towards understanding the neurologic functions of NF2 and TSC genes and treating disease manifestations associated with these diseases. ..
- Characterization of Dystonia Gene and ProteinXandra Breakefield; Fiscal Year: 2006..Dystonia represents a special class of neurologic diseases, which do not manifest apparent neurodegeneration. This class of diseases may be amenable to therapy informed by the molecular etiology of dysfunction at the cellular level. ..
- Visualizing synaptic circuitry in the retinaXandra Breakefield; Fiscal Year: 2006..abstract_text> ..