Richard Bouley

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. doi request reprint Corin: a key protein of an adaptive renal mechanism to respond to salt variation?
    Richard Bouley
    Program in Membrane Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Kidney Int 82:7-8. 2012
  2. ncbi request reprint Functional role of the NPxxY motif in internalization of the type 2 vasopressin receptor in LLC-PK1 cells
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Am J Physiol Cell Physiol 285:C750-62. 2003
  3. ncbi request reprint Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal degradation pathway
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital East, 149 13th St, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 288:C1390-401. 2005
  4. pmc Heterologous downregulation of vasopressin type 2 receptor is induced by transferrin
    Richard Bouley
    1Nephrology Division, MGH Center for Systems Biology, Program in Membrane Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 304:F553-64. 2013
  5. pmc Calcitonin has a vasopressin-like effect on aquaporin-2 trafficking and urinary concentration
    Richard Bouley
    Massachusetts General Hospital, Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Simches Research Center, Room 8202, 185 Cambridge Street, Boston, MA 02114, USA
    J Am Soc Nephrol 22:59-72. 2011
  6. pmc Acute hypertonicity alters aquaporin-2 trafficking and induces a MAPK-dependent accumulation at the plasma membrane of renal epithelial cells
    Udo Hasler
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts 02114 2790, USA
    J Biol Chem 283:26643-61. 2008
  7. ncbi request reprint Characterization of single channel currents from primary cilia of renal epithelial cells
    Malay K Raychowdhury
    Renal Unit, Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA
    J Biol Chem 280:34718-22. 2005
  8. ncbi request reprint Aquaporin 2 (AQP2) and vasopressin type 2 receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in 'endocytosis-resistant' membrane domains after vasopressin treatment
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Biol Cell 98:215-32. 2006
  9. ncbi request reprint Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra)
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Rm 8207, Massachusetts General Hospital East, 149 13th St Charlestown, MA 02129, USA
    Am J Physiol Renal Physiol 288:F1103-12. 2005
  10. pmc Phosphorylation events and the modulation of aquaporin 2 cell surface expression
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Curr Opin Nephrol Hypertens 17:491-8. 2008

Collaborators

Detail Information

Publications31

  1. doi request reprint Corin: a key protein of an adaptive renal mechanism to respond to salt variation?
    Richard Bouley
    Program in Membrane Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Kidney Int 82:7-8. 2012
    ..This suggests that corin has an important role in a new adaptive mechanism of the response to variations of salt in the diet...
  2. ncbi request reprint Functional role of the NPxxY motif in internalization of the type 2 vasopressin receptor in LLC-PK1 cells
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Am J Physiol Cell Physiol 285:C750-62. 2003
    ..However, this motif is not essential for the apical/basolateral sorting and polarized distribution of the V2R in LLC-PK1a cells or for adenylyl cyclase-mediated signal transduction...
  3. ncbi request reprint Downregulation of the vasopressin type 2 receptor after vasopressin-induced internalization: involvement of a lysosomal degradation pathway
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital East, 149 13th St, Charlestown, MA 02129, USA
    Am J Physiol Cell Physiol 288:C1390-401. 2005
    ..This degradative pathway may be an adaptive response to allow receptor-ligand association in the hypertonic and acidic environment of the renal medulla...
  4. pmc Heterologous downregulation of vasopressin type 2 receptor is induced by transferrin
    Richard Bouley
    1Nephrology Division, MGH Center for Systems Biology, Program in Membrane Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 304:F553-64. 2013
    ..It also provides new insights into urine-concentrating defects observed in rat models of hemochromatosis...
  5. pmc Calcitonin has a vasopressin-like effect on aquaporin-2 trafficking and urinary concentration
    Richard Bouley
    Massachusetts General Hospital, Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Simches Research Center, Room 8202, 185 Cambridge Street, Boston, MA 02114, USA
    J Am Soc Nephrol 22:59-72. 2011
    ..This suggests that calcitonin has a potential therapeutic use in nephrogenic diabetes insipidus...
  6. pmc Acute hypertonicity alters aquaporin-2 trafficking and induces a MAPK-dependent accumulation at the plasma membrane of renal epithelial cells
    Udo Hasler
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts 02114 2790, USA
    J Biol Chem 283:26643-61. 2008
    ..This may have important implications on adaptational processes governing transcellular water flux and/or cell survival under extreme conditions of hypertonicity...
  7. ncbi request reprint Characterization of single channel currents from primary cilia of renal epithelial cells
    Malay K Raychowdhury
    Renal Unit, Massachusetts General Hospital East, Charlestown, Massachusetts 02129, USA
    J Biol Chem 280:34718-22. 2005
    ..Ion channel activity in the primary cilium of renal cells may be an important component of its role as a sensory transducer...
  8. ncbi request reprint Aquaporin 2 (AQP2) and vasopressin type 2 receptor (V2R) endocytosis in kidney epithelial cells: AQP2 is located in 'endocytosis-resistant' membrane domains after vasopressin treatment
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    Biol Cell 98:215-32. 2006
    ..In the present study, we further investigated AQP2 accumulation at the cell surface, and compared it with V2R (VP type 2 receptor) trafficking using cells that express epitope-tagged AQP2 and V2R...
  9. ncbi request reprint Stimulation of AQP2 membrane insertion in renal epithelial cells in vitro and in vivo by the cGMP phosphodiesterase inhibitor sildenafil citrate (Viagra)
    Richard Bouley
    Program in Membrane Biology and Renal Unit, Rm 8207, Massachusetts General Hospital East, 149 13th St Charlestown, MA 02129, USA
    Am J Physiol Renal Physiol 288:F1103-12. 2005
    ....
  10. pmc Phosphorylation events and the modulation of aquaporin 2 cell surface expression
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Curr Opin Nephrol Hypertens 17:491-8. 2008
    ....
  11. pmc Simvastatin enhances aquaporin-2 surface expression and urinary concentration in vasopressin-deficient Brattleboro rats through modulation of Rho GTPase
    Wei Li
    Center for Systems Biology, Program in Membrane Biology, Division of Nephrology, Dept of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 301:F309-18. 2011
    ..Our study provides an alternative mechanism to regulate AQP2 trafficking, bypassing the VP-vasopressin receptor signaling pathway...
  12. pmc Effects of the renal medullary pH and ionic environment on vasopressin binding and signaling
    Elena A Zalyapin
    Program in Membrane Biology and Nephrology Division, MGH Center for Systems Biology, Boston, Massachusetts 02114, USA
    Kidney Int 74:1557-67. 2008
    ..While the gradient is important for urinary concentration, it may also play a substantial role in fine-tuning of the vasopressin response through the V2R...
  13. ncbi request reprint Inhibition of endocytosis causes phosphorylation (S256)-independent plasma membrane accumulation of AQP2
    Hua Lu
    Program in Membrane Biology, Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 286:F233-43. 2004
    ....
  14. pmc Visualizing microtubule-dependent vasopressin type 2 receptor trafficking using a new high-affinity fluorescent vasopressin ligand
    Sylvia Chen
    Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Endocrinology 152:3893-904. 2011
    ..We conclude that the new VP(TMR) ligand is suitable for dissecting V2R and VP internalization and trafficking in cells, and that V2R trafficking and down-regulation is an MT-dependent mechanism...
  15. pmc Bypassing vasopressin receptor signaling pathways in nephrogenic diabetes insipidus
    Richard Bouley
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Semin Nephrol 28:266-78. 2008
    ....
  16. ncbi request reprint Membrane organization and function of M1 and M23 isoforms of aquaporin-4 in epithelial cells
    Claudia Silberstein
    Renal Unit and Program in Membrane Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 287:F501-11. 2004
    ....
  17. pmc Vasopressin-induced differential stimulation of AQP4 splice variants regulates the in-membrane assembly of orthogonal arrays
    Alfred N Van Hoek
    Simches Research Center, 8 208, 185 Cambridge St, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 296:F1396-404. 2009
    ....
  18. ncbi request reprint Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking
    Hua A J Lu
    Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    J Biol Chem 282:28721-32. 2007
    ..To summarize, we have identified the 70-kDa heat shock proteins as a AQP2 interactors and have shown for hsc70 that this interaction is involved in AQP2 trafficking...
  19. pmc Basolateral targeting and microtubule-dependent transcytosis of the aquaporin-2 water channel
    Naofumi Yui
    Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 304:C38-48. 2013
    ..Furthermore, our data indicate that protein polarity data obtained from biotinylation assays, which require cells to be cooled to 4°C during the labeling procedure, should be interpreted with caution...
  20. pmc A fluorimetry-based ssYFP secretion assay to monitor vasopressin-induced exocytosis in LLC-PK1 cells expressing aquaporin-2
    Paula Nunes
    Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 295:C1476-87. 2008
    ..These results support the idea that, in addition to its role in reducing AQP2 endocytosis, VP increases AQP2 exocytosis...
  21. pmc The phosphorylation state of serine 256 is dominant over that of serine 261 in the regulation of AQP2 trafficking in renal epithelial cells
    Hua Jenny Lu
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 295:F290-4. 2008
    ..The potential role of S261 phosphorylation/dephosphorylation in vasopressin action remains to be determined...
  22. pmc New insights into the dynamic regulation of water and acid-base balance by renal epithelial cells
    Dennis Brown
    MGH Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Simches Research Center, Massachusetts General Hospital, Boston, MA 02114, USA
    Am J Physiol Cell Physiol 302:C1421-33. 2012
    ..This review summarizes recent advances in our understanding of these fundamental processes...
  23. pmc Sustained cyclic AMP production by parathyroid hormone receptor endocytosis
    Sebastien Ferrandon
    Endocrine Unit, Division of Nephrology, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Nat Chem Biol 5:734-42. 2009
    ..Such marked differences suggest a mechanism by which PTH and PTHrP induce differential responses, and these results indicate that the central tenet that cAMP production originates exclusively at the cell membrane must be revised...
  24. pmc Physical and functional connection between auxilin and dynamin during endocytosis
    Sanja Sever
    Renal Unit, Nephrology Division, Department of Medicine, Harvard Medical School and Massachusetts General Hospital, Charlestown, MA 02129, USA
    EMBO J 25:4163-74. 2006
    ..Together, our findings support the model that the GTP-bound conformation of dynamin tetramers stimulates formation of constricted coated pits at the plasma membrane by regulating the chaperone activity of hsc70/auxilin...
  25. ncbi request reprint Aquaporin-2 localization in clathrin-coated pits: inhibition of endocytosis by dominant-negative dynamin
    Tian Xiao Sun
    Program in Membrane Biology and Renal Unit, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114, USA
    Am J Physiol Renal Physiol 282:F998-1011. 2002
    ..We propose that the IMP clusters previously associated with vasopressin action represent sites of dynamin-dependent, clathrin-mediated endocytosis in which AQP2 is concentrated before internalization...
  26. pmc Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress
    Aditi U Gurkar
    Cutaneous Biology Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    Nat Commun 4:2189. 2013
    ..These results show that ROCK1 acts as a prominent upstream regulator of Beclin1-mediated autophagy and maintains a homeostatic balance between apoptosis and autophagy. ..
  27. pmc Angiotensin II and hypertonicity modulate proximal tubular aquaporin 1 expression
    Richard Bouley
    Center for Systems Biology, Program in Membrane Biology, Nephrology Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    Am J Physiol Renal Physiol 297:F1575-86. 2009
    ....
  28. pmc AQP2 is necessary for vasopressin- and forskolin-mediated filamentous actin depolymerization in renal epithelial cells
    Naofumi Yui
    Massachusetts General Hospital Center for Systems Biology, Program in Membrane Biology and Nephrology Division, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Biol Open 1:101-8. 2012
    ..This is the first report showing that VP/FK mediated F-actin depolymerization is dependent on AQP2 protein expression in renal epithelial cells, and that this is not dependent on the polarity of AQP2 membrane insertion...
  29. pmc Dragon enhances BMP signaling and increases transepithelial resistance in kidney epithelial cells
    Yin Xia
    Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Am Soc Nephrol 21:666-77. 2010
    ..Taken together, these results suggest that Dragon may enhance BMP signaling in renal tubular epithelial cells and maintain normal renal physiology...
  30. ncbi request reprint Alix (AIP1) is a vasopressin receptor (V2R)-interacting protein that increases lysosomal degradation of the V2R
    Xianhua Yi
    Cellular Biophysics Laboratory, Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas, USA
    Am J Physiol Renal Physiol 292:F1303-13. 2007
    ..These data suggest that Alix increases the rate of lysosomal degradation of V2R and may play an important regulatory role in the VP response by modulating V2R downregulation...
  31. ncbi request reprint Hypertonicity is involved in redirecting the aquaporin-2 water channel into the basolateral, instead of the apical, plasma membrane of renal epithelial cells
    Bas W M van Balkom
    Department of Cell Physiology, Nijmegen Center for Molecular Life Sciences, UMC St Radboud, The Netherlands
    J Biol Chem 278:1101-7. 2003
    ..These data reveal for the first time that chronic hypertonicity can program cells in vitro and in vivo to change the insertion of a protein into the basolateral membrane instead of the apical membrane...