Affiliation: Harvard University
Hosu B, Berg H. CW and CCW Conformations of the E. coli Flagellar Motor C-Ring Evaluated by Fluorescence Anisotropy. Biophys J. 2018;114:641-649 pubmed publisher
Shrivastava A, Lele P, Berg H. A rotary motor drives Flavobacterium gliding. Curr Biol. 2015;25:338-41 pubmed publisher
..However, we found that a gliding motor runs at constant speed rather than at constant torque. Now, there are three rotary motors powered by protonmotive force: the bacterial flagellar motor, the Fo ATP synthase, and the gliding motor. ..
Shrivastava A, Berg H. Towards a model for Flavobacterium gliding. Curr Opin Microbiol. 2015;28:93-7 pubmed publisher
..Recent advances in F. johnsoniae research include the discovery of mobile cell-surface adhesins and rotary motors. The puzzle is how rotary motion leads to linear motion. We suggest a possible mechanism, inspired by the snowmobile. ..
Hosu B, Nathan V, Berg H. Internal and external components of the bacterial flagellar motor rotate as a unit. Proc Natl Acad Sci U S A. 2016;113:4783-7 pubmed publisher
..Here, by using polarized light to bleach and probe an internal YFP-FliN fusion, we show that the innermost components of the cytoplasmic ring rotate at a rate similar to that of the hook. ..
Turner L, Ping L, Neubauer M, Berg H. Visualizing Flagella while Tracking Bacteria. Biophys J. 2016;111:630-639 pubmed publisher
..If a smaller fraction of filaments were involved in tumbles, the tumble intervals were shorter and the angles between runs were smaller. ..
Shrivastava A, Roland T, Berg H. The Screw-Like Movement of a Gliding Bacterium Is Powered by Spiral Motion of Cell-Surface Adhesins. Biophys J. 2016;111:1008-13 pubmed publisher
..Thus, if SprB were to adhere to the glass rather than to a nanoparticle, the cell would move forward along a right-handed trajectory, as observed, but in a direction opposite to that of the nanoparticle. ..
Turner L, Berg H. Labeling Bacterial Flagella with Fluorescent Dyes. Methods Mol Biol. 2018;1729:71-76 pubmed publisher
..For imaging in vivo, blurring can be prevented by strobing the light source or by using a camera with a fast shutter. The former method is preferred, since it minimizes bleaching. ..
Branch R, Sayegh M, Shen C, Nathan V, Berg H. Adaptive remodelling by FliN in the bacterial rotary motor. J Mol Biol. 2014;426:3314-3324 pubmed publisher
..The behaviour of FliM and FliN is consistent with a kinetic and stoichiometric model that contradicts the traditional view of a packed, rigid motor architecture. ..