Research Topics
| FREDERICK AUSUBELSummaryAffiliation: Harvard University Country: USA Publications
Research Grants
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Publications
Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorialDaniel G Lee
Department of Molecular Biology, Massachusetts General Hospital, Cambridge Street, Boston, Massachusetts 02114, USA
Genome Biol 7:R90. 2006..We therefore propose that virulence in this organism is both multifactorial and combinatorial, the result of a pool of pathogenicity-related genes that interact in various combinations in different genetic backgrounds...
Are innate immune signaling pathways in plants and animals conserved?Frederick M Ausubel
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
Nat Immunol 6:973-9. 2005....- Caenorhabditis elegans as a model host for Staphylococcus aureus pathogenesisCosti D Sifri
Division of Infectious Diseases, Department of Molecular Biology, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
Infect Immun 71:2208-17. 2003..These results suggest that key aspects of S. aureus pathogenesis have been conserved, irrespective of the host, and that specific C. elegans host factors can alter susceptibility to this gram-positive human pathogen... - Distinct pathogenesis and host responses during infection of C. elegans by P. aeruginosa and S. aureusJavier E Irazoqui
Program of Developmental Immunology, Department of Pediatrics, Massachusetts General Hospital, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Pathog 6:e1000982. 2010..aeruginosa nor the S. aureus-triggered response requires canonical TLR signaling, they imply the existence of unidentified mechanisms for pathogen detection in C. elegans, with potentially conserved roles also in mammals... - Caenorhabditis elegans as a host for the study of host-pathogen interactionsAlejandro Aballay
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, USA
Curr Opin Microbiol 5:97-101. 2002..elegans mutants that exhibit altered susceptibility to pathogen attack. The use of Caenorhabditis elegans as the host for a variety of human pathogens is discussed... - Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulenceHemantha Kulasakara
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 103:2839-44. 2006.... - A metasystem of framework model organisms to study emergence of new host-microbe adaptationsSuresh Gopalan
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, USA
PLoS ONE 3:e3891. 2008..This would aid in the study of emergence and progression of host-microbe maladaptations in a controlled environment... - RESISTANCE TO FUSARIUM OXYSPORUM 1, a dominant Arabidopsis disease-resistance gene, is not race specificAndrew C Diener
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
Genetics 171:305-21. 2005..matthioli, f. conglutinans, and f. raphani. Thus, RFO1 encodes a novel type of dominant disease-resistance protein that confers resistance to a broad spectrum of Fusarium races... - Identification of virulence genes in a pathogenic strain of Pseudomonas aeruginosa by representational difference analysisJi Young Choi
Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
J Bacteriol 184:952-61. 2002..This suggests that the increased virulence of P. aeruginosa strain PA14 compared to PAO1 may relate to specific genomic differences identifiable by RDA... - Role for beta-catenin and HOX transcription factors in Caenorhabditis elegans and mammalian host epithelial-pathogen interactionsJavier E Irazoqui
Department of Genetics, Harvard Medical School, and Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 105:17469-74. 2008..Overexpression of human homologs of egl-5 modulated NF-kappaB-dependent TLR2 signaling in epithelial cells. These data suggest that beta-catenin and homeobox genes play an important and conserved role in innate immune defense... - Virulence effect of Enterococcus faecalis protease genes and the quorum-sensing locus fsr in Caenorhabditis elegans and miceCosti D Sifri
Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
Infect Immun 70:5647-50. 2002..These data show that extracellular proteases and the quorum-sensing fsr system are important for E. faecalis virulence in two highly divergent hosts: nematodes and mice... - A conserved p38 MAP kinase pathway in Caenorhabditis elegans innate immunityDennis H Kim
Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
Science 297:623-6. 2002..These data suggest that this MAP kinase signaling cassette represents an ancient feature of innate immune responses in evolutionarily diverse species... - p38 MAPK regulates expression of immune response genes and contributes to longevity in C. elegansEmily R Troemel
Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Genet 2:e183. 2006..The contribution of the PMK-1 pathway to the enhanced lifespan of daf-2 mutants suggests that innate immunity is an important determinant of longevity... - Identification of novel antimicrobials using a live-animal infection modelTerence I Moy
Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 103:10414-9. 2006..Our findings indicate that the whole-animal C. elegans screen identifies not only traditional antibiotics, but also compounds that target bacterial virulence or stimulate host defense... - Cytotoxicity of hydrogen peroxide produced by Enterococcus faeciumTerence I Moy
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, 02114, USA
Infect Immun 72:4512-20. 2004..faecium-mediated killing. These results suggest that hydrogen peroxide produced by E. faecium has cytotoxic effects and highlight the utility of C. elegans pathogenicity models for identifying bacterial virulence factors... - The art of serendipity: killing of Caenorhabditis elegans by human pathogens as a model of bacterial and fungal pathogenesisEleftherios Mylonakis
Harvard Medical School, Massachusetts General Hospital, Division of Infectious Diseases, 55 Fruit Street, Gray 5, GRJ 504, Boston, MA 02114, USA
Expert Rev Anti Infect Ther 1:167-73. 2003..In contrast, the Caenorhabditis elegans model allows rapid identification of mutants in microbial genes associated with pathogenesis and then these phenotypes can be confirmed in a relevant mammalian model... - Evolutionary perspectives on innate immunity from the study of Caenorhabditis elegansDennis H Kim
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA
Curr Opin Immunol 17:4-10. 2005..We anticipate that the study of pathogen resistance in C. elegans will continue to provide evolutionary and mechanistic insights into the signal transduction and physiology of innate immunity... - Staphylococcus aureus virulence factors identified by using a high-throughput Caenorhabditis elegans-killing modelJakob Begun
Department of Molecular Biology, Massachusetts General Hospital, 50 Blossom Street, Boston, MA 02114, USA
Infect Immun 73:872-7. 2005..Interestingly, Tn917 was shown to have a very strong bias for insertions near the terminus of DNA replication... - Activation of defense response pathways by OGs and Flg22 elicitors in Arabidopsis seedlingsCarine Denoux
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, 185 Cambridge St, Simches 7700, Boston, MA 02114, USA
Mol Plant 1:423-45. 2008..Expression patterns of amino-cyclopropane-carboxylate synthase genes also implicate ethylene biosynthesis in regulation of the late innate immune response... - Long-lived C. elegans daf-2 mutants are resistant to bacterial pathogensDanielle A Garsin
Department of Genetics, Massachusetts General Hospital, Boston, MA 02114, USA
Science 300:1921. 2003 - Conjugating berberine to a multidrug efflux pump inhibitor creates an effective antimicrobialAnthony R Ball
Department of Biology and Antimicrobial Discovery Center, Northeastern University, Boston, Massachusetts 02115, USA
ACS Chem Biol 1:594-600. 2006..The hybrid molecule showed good efficacy in a Caenorhabditis elegans model of enterococcal infection, curing worms of the pathogen... 
Models of Caenorhabditis elegans infection by bacterial and fungal pathogensJennifer R Powell
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA
Methods Mol Biol 415:403-27. 2008..Here, we describe pathogen assays for a selection of the most commonly studied bacterial and fungal pathogens using the C. elegans model system...- Arabidopsis local resistance to Botrytis cinerea involves salicylic acid and camalexin and requires EDS4 and PAD2, but not SID2, EDS5 or PAD4Simone Ferrari
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, USA
Plant J 35:193-205. 2003..cinerea requires ethylene-, jasmonate-, and SA-mediated signaling, that the SA affecting this resistance does not require ICS1 and is likely synthesized via PAL, and that camalexin limits lesion development... - The Enterococcus faecalis fsrB gene, a key component of the fsr quorum-sensing system, is associated with virulence in the rabbit endophthalmitis modelEleftherios Mylonakis
Division of Infectious Diseases, Harvard Medical School, Boston, Massachusetts 02114, USA
Infect Immun 70:4678-81. 2002..Complementation of mutation restored virulence. These data corroborate the role of fsrB in E. faecalis pathogenesis and suggest that the rabbit endophthalmitis model can be used to study the in vivo role of quorum sensing... - Use of the Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesisSachiko Miyata
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
Infect Immun 71:2404-13. 2003..mellonella model and the results of cytopathology assays performed with a mammalian tissue culture system validated the use of G. mellonella for the study of the P. aeruginosa TTSS... - Caenorhabditis elegans innate immune response triggered by Salmonella enterica requires intact LPS and is mediated by a MAPK signaling pathwayAlejandro Aballay
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, 02114, Boston, MA, USA
Curr Biol 13:47-52. 2003..However, a presumptive C. elegans TOLL signaling pathway did not appear to be required for the PCD response to Salmonella. These results establish a PMK-1-dependant PCD pathway as a C. elegans innate immune response to Salmonella... - Hypersusceptibility of cystic fibrosis mice to chronic Pseudomonas aeruginosa oropharyngeal colonization and lung infectionFadie T Coleman
Channing Laboratory, Department of Medicine, Brigham and Women s Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:1949-54. 2003..aeruginosa colonization and infection and can be used for evaluations of lung pathophysiology, bacterial virulence, and development of therapies aimed at treating CF lung disease... - The G protein-coupled receptor FSHR-1 is required for the Caenorhabditis elegans innate immune responseJennifer R Powell
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 106:2782-7. 2009..FSHR-1 may act generally to boost the nematode immune response, or it may function as a pathogen receptor... - Comparing insertion libraries in two Pseudomonas aeruginosa strains to assess gene essentialityNicole T Liberati
Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA
Methods Mol Biol 416:153-69. 2008.... - An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutantsNicole T Liberati
Department of Genetics, Harvard Medical School, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 103:2833-8. 2006..Comparison of the genes disrupted in the PA14 transposon insertion library with an independently constructed insertion library in P. aeruginosa strain PAO1 provides an estimate of the number of P. aeruginosa essential genes... - High-throughput screen for novel antimicrobials using a whole animal infection modelTerence I Moy
Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
ACS Chem Biol 4:527-33. 2009..elegans animals but do not affect the growth of the pathogen in vitro, thus acting by a mechanism of action distinct from antibiotics currently in clinical use... - Using PATIMDB to create bacterial transposon insertion mutant librariesJonathan M Urbach
Massachusetts General Hospital, Boston, Massachusetts, USA
Curr Protoc Mol Biol . 2009..The protocols in this unit describe installation and use of PATIMDB software... - Prospects for plant-derived antibacterialsKim Lewis
Antimicrobial Discovery Center and Department of Biology, Northeastern University, Boston, Massachusetts 02115, USA
Nat Biotechnol 24:1504-7. 2006..Can weakly active phytochemicals be combined synergistically to produce new antibacterial treatments?.. - Microsporidia are natural intracellular parasites of the nematode Caenorhabditis elegansEmily R Troemel
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA
PLoS Biol 6:2736-52. 2008..elegans and its natural microsporidian parasites provides a system in which to dissect intracellular intestinal infection in vivo and insight into the diversity of pathogenic mechanisms used by intracellular microbes... - Tandemly duplicated Arabidopsis genes that encode polygalacturonase-inhibiting proteins are regulated coordinately by different signal transduction pathways in response to fungal infectionSimone Ferrari
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
Plant Cell 15:93-106. 2003..Therefore, gene duplication followed by the divergence of promoter regions may result in different modes of regulation of similar defensive proteins, thereby enhancing the likelihood of defense gene activation during pathogen infection... - Pseudomonas biofilm formation and antibiotic resistance are linked to phenotypic variationEliana Drenkard
Department of Genetics, Harvard Medical School, Massahusetts General Hospital, Boston, MA 02114, USA
Nature 416:740-3. 2002..Compounds that affect PvrR function could have an important role in the treatment of CF infections... - Requirement for a conserved Toll/interleukin-1 resistance domain protein in the Caenorhabditis elegans immune responseNicole T Liberati
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, USA
Proc Natl Acad Sci U S A 101:6593-8. 2004..These data reveal the involvement of a previously uncharacterized, evolutionarily conserved TIR domain protein in innate immunity that is functionally distinct from other known TIR domain signaling adapters... - Challenge of Drosophila melanogaster with Cryptococcus neoformans and role of the innate immune responseYiorgos Apidianakis
Department of Surgery, Massachusetts General Hospital. Shriner's Burns Institute, Boston, Massachusetts 02114, USA
Eukaryot Cell 3:413-9. 2004..However, the Toll pathway was necessary for the clearance of C. neoformans introduced directly into the hemolymph of D. melanogaster and for the survival of systemically infected flies... - Strains of Escherichia coli O157:H7 differ primarily by insertions or deletions, not single-nucleotide polymorphismsIndira T Kudva
Division of Infectious Diseases, Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
J Bacteriol 184:1873-9. 2002..coli K-12 (O islands), suggesting that strain-to-strain variation occurs in these O islands. These results may be utilized to devise novel strain-typing tools for this pathogen... - Glucosinolate metabolites required for an Arabidopsis innate immune responseNicole K Clay
Department of Genetics, Harvard Medical School, Massachusetts General Hospital, Boston, MA 02114, USA
Science 323:95-101. 2009..Our study shows that well-studied plant metabolites, previously identified as important in avoiding damage by herbivores, are also required as a component of the plant defense response against microbial pathogens... - DAF-16-dependent suppression of immunity during reproduction in Caenorhabditis elegansSachiko Miyata
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
Genetics 178:903-18. 2008..The timing of DAF-16-dependent gene activation in sterile mutants coincides with the onset of embryonic development in wild-type animals, suggesting that signals from developing embryos normally downregulate the immune response... - Polymorphic amplified typing sequences provide a novel approach to Escherichia coli O157:H7 strain typingIndira T Kudva
Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
J Clin Microbiol 40:1152-9. 2002..These data suggest that typing by PATS may provide a simple procedure for strain typing of O157 and other bacteria and that further evaluation of the utility of this method for epidemiologic investigations is warranted... - Integration of Caenorhabditis elegans MAPK pathways mediating immunity and stress resistance by MEK-1 MAPK kinase and VHP-1 MAPK phosphataseDennis H Kim
Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, Boston, 02114, USA
Proc Natl Acad Sci U S A 101:10990-4. 2004..In addition, these data point to mechanisms in multicellular organisms by which signals transduced by distinct MAPK pathways may be subject to physiological integration at the level of regulation of MAPK activity by MAPKKs and MKPs... - Prevention and control of pests and diseasesJenifer Bush
Department of Molecular Biology, Massachusetts General Hospital, Boston, USA
Methods Mol Biol 323:13-25. 2006..Bacterial and viral infections of Arabidopsis, though they do occur, tend to be less common and can usually be controlled by maintaining optimal growth conditions and promptly disposing of dead or diseased plant material... - MAP kinase signalling cascade in Arabidopsis innate immunityTsuneaki Asai
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02114, USA
Nature 415:977-83. 2002..Activation of this MAPK cascade confers resistance to both bacterial and fungal pathogens, suggesting that signalling events initiated by diverse pathogens converge into a conserved MAPK cascade... - Evolution of host innate defence: insights from Caenorhabditis elegans and primitive invertebratesJavier E Irazoqui
Department of Pediatrics, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
Nat Rev Immunol 10:47-58. 2010..What, therefore, do we know about host defence mechanisms in C. elegans and what can they tell us about innate immunity in higher organisms?.. - Innate immune responses activated in Arabidopsis roots by microbe-associated molecular patternsYves A Millet
Department of Genetics, Harvard Medical School, Boston, MA 02114, U SA
Plant Cell 22:973-90. 2010..These experiments demonstrate the presence of highly orchestrated and tissue-specific MAMP responses in roots and potential pathogen-encoded mechanisms to block these MAMP-elicited signaling pathways... - Galleria mellonella as a model system to study Cryptococcus neoformans pathogenesisEleftherios Mylonakis
Division of Infectious Diseases, Massachusetts General Hospital, Gray Jackson 504, 55 Fruit St, Boston, Massachusetts 02114, USA
Infect Immun 73:3842-50. 2005..The G. mellonella-C. neoformans pathogenicity model may be a substitute for mammalian models of infection with C. neoformans and may facilitate the in vivo study of fungal virulence and efficacy of antifungal therapies... - Cryptococcus neoformans Kin1 protein kinase homologue, identified through a Caenorhabditis elegans screen, promotes virulence in mammalsEleftherios Mylonakis
Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA
Mol Microbiol 54:407-19. 2004..These findings show that the C. neoformans Kin1 kinase homologue is required for full virulence in disparate hosts and that C. elegans can be used as a substitute host to identify novel factors involved in fungal pathogenesis in mammals... - Signals involved in Arabidopsis resistance to Trichoplusia ni caterpillars induced by virulent and avirulent strains of the phytopathogen Pseudomonas syringaeJianping Cui
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138, USA
Plant Physiol 129:551-64. 2002..ni resistance and overrides the SA-mediated increase in T. ni susceptibility, and a SA-independent systemic response induced by virulent pathogens that leads to enhanced susceptibility to T. ni... - Staphylococcal biofilm exopolysaccharide protects against Caenorhabditis elegans immune defensesJakob Begun
Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America
PLoS Pathog 3:e57. 2007.... - Attenuation of Pseudomonas aeruginosa virulence by medicinal plants in a Caenorhabditis elegans model systemAllison Adonizio
Department of Biological Sciences, College of Arts and Sciences, Florida International University, Miami, FL 33199, USA
J Med Microbiol 57:809-13. 2008..All extracts inhibited nematode death by P. aeruginosa without host toxicity, indicating their potential for further development as anti-infectives... - Contribution of gelatinase, serine protease, and fsr to the pathogenesis of Enterococcus faecalis endophthalmitisMichael Engelbert
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
Infect Immun 72:3628-33. 2004..This increased attenuation suggests that there are possible additional pleiotropic effects of the defect in fsr on expression of traits contributing to the pathogenesis of enterococcal infection... - Caenorhabditis elegans-based screen identifies Salmonella virulence factors required for conserved host-pathogen interactionsJennifer L Tenor
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710 USA
Curr Biol 14:1018-24. 2004.... - Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesisEleftherios Mylonakis
Division of Infectious Diseases and Department of Molecular Biology, Massachusetts General Hospital, Boston 02114, USA
Proc Natl Acad Sci U S A 99:15675-80. 2002..neoformans with environmental predators such as free-living nematodes and amoebae and suggest that C. elegans can be used as a simple model host in which C. neoformans pathogenesis can be readily studied... - The worm has turned--microbial virulence modeled in Caenorhabditis elegansCosti D Sifri
Division of Infectious Diseases and International Health, University of Virginia Health System, MR4, Charlottesville, VA 22908, USA
Trends Microbiol 13:119-27. 2005..C. elegans-based pathogenesis systems hold the potential to simultaneously explore the molecular genetic determinants of both pathogen virulence and host defense... - Animal models for host-pathogen interactionsBruno Lemaitre
Curr Opin Microbiol 11:249-50. 2008 - A high-throughput, near-saturating screen for type III effector genes from Pseudomonas syringaeJeff H Chang
Department of Biology CB 3280, Carolina Center for Genome Sciences, University of North Carolina, Chapel Hill, NC 27599, USA
Proc Natl Acad Sci U S A 102:2549-54. 2005..syringae pathovars and show that type III effector protein suites are highly variable in this pathogen, presumably reflecting the evolutionary selection imposed by the various host plants... - Pseudomonas syringae manipulates systemic plant defenses against pathogens and herbivoresJianping Cui
Department of Organismic and Evolutionary Biology, Harvard University, 26 Oxford Street, Cambridge, MA 02138, USA
Proc Natl Acad Sci U S A 102:1791-6. 2005..Rather, consistent with its role as a JA mimic, COR induces systemic resistance to T. ni. These data highlight the complexity of defense signaling interactions among plants, pathogens, and herbivores... - Construction of an Enterococcus faecalis Tn917-mediated-gene-disruption library offers insight into Tn917 insertion patternsDanielle A Garsin
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center at Houston, 6431 Fannin Street, JFB 1 711, Houston, TX 77030, USA
J Bacteriol 186:7280-9. 2004..2 Mb), the only E. faecalis strain whose sequence is in the public domain, over 10% of the Tn917 insertions appear to be in a OG1RF-specific sequence, suggesting that there are significant genomic differences among E. faecalis strains... - Mediation of pathogen resistance by exudation of antimicrobials from rootsHarsh P Bais
Department of Horticulture and Landscape Architecture, and Center for Rhizosphere Biology, Colorado State University, Fort Collins, Colorado 80523-1173, USA
Nature 434:217-21. 2005..We also show that the ability of this P. syringae strain to block antimicrobial exudation is dependent on the type III secretory system... - Antifungal chemical compounds identified using a C. elegans pathogenicity assayJulia Breger
Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America
PLoS Pathog 3:e18. 2007..Compounds identified in the screen that affect the virulence of Candida in vivo can potentially be used as "probe compounds" and may have antifungal activity against other fungi... - Resistance to Botrytis cinerea induced in Arabidopsis by elicitors is independent of salicylic acid, ethylene, or jasmonate signaling but requires PHYTOALEXIN DEFICIENT3Simone Ferrari
Dipartimento Territorio e Sistemi Agro Forestali, Universita degli Studi di Padova, 23 35020 Legnaro, Italy
Plant Physiol 144:367-79. 2007.... - Characterization of the integrated filamentous phage Pf5 and its involvement in small-colony formationMarlies J Mooij
Department of Medical Microbiology and Infection Control, VU Medical Centre, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands
Microbiology 153:1790-8. 2007..aeruginosa SCVs... - BifA, a cyclic-Di-GMP phosphodiesterase, inversely regulates biofilm formation and swarming motility by Pseudomonas aeruginosa PA14Sherry L Kuchma
Department of Microbiology and Immunology, Dartmouth Medical School, Rm 505, Vail Building, North College St, Hanover, NH 03755, USA
J Bacteriol 189:8165-78. 2007..The DeltabifA mutation also results in decreased flagellar reversals. Based on epistasis studies with the previously described sadB gene, we propose that BifA functions upstream of SadB in the control of biofilm formation and swarming... - The Caenorhabditis elegans MAPK phosphatase VHP-1 mediates a novel JNK-like signaling pathway in stress responseTomoaki Mizuno
Department of Molecular Biology, Graduate School of Science, Institute for Advanced Research, Nagoya University, Nagoya, Japan
EMBO J 23:2226-34. 2004..These results suggest that VHP-1 plays a pivotal role in the integration and fine-tuning of the stress response regulated by the KGB-1 MAPK pathway... - Exploiting amoeboid and non-vertebrate animal model systems to study the virulence of human pathogenic fungiEleftherios Mylonakis
PLoS Pathog 3:e101. 2007..This review aims to assist researchers in identifying appropriate invertebrate systems for the study of particular aspects of fungal pathogenesis...
Research Grants
- GENETIC ANALYSIS OF THE PLANT DEFENSE RESPONSEFREDERICK AUSUBEL; Fiscal Year: 2007..We will identify signaling pathways that lead to the biosynthesis of antimicrobial compounds. Finally, we will enter the data from this project into IMDS, a public, web-accessible relational database. ..
- Novel whole-animal screens for anti-microbialsFREDERICK AUSUBEL; Fiscal Year: 2007..2) Develop a high throughput C. elegans - S. enterica curing assay. 3) Develop a high throughput screen for identifying MDR pump inhibitors in the C. elegans curing assays. ..
- Studies of Caenorhabditis elegans innate immunityFREDERICK AUSUBEL; Fiscal Year: 2007..elegans immune response and to delineate the relationship between longevity and pathogen resistance. ..
- Novel whole-animal screens for anti-microbialsFREDERICK AUSUBEL; Fiscal Year: 2009..2) Develop a high throughput C. elegans - S. enterica curing assay. 3) Develop a high throughput screen for identifying MDR prump inhibitors in the C. elegans curing assays. ..
- Studies of Caenorhabditis elegans innate immunityFREDERICK AUSUBEL; Fiscal Year: 2009..elegans immune response and to delineate the relationship between longevity and pathogen resistance. ..
- GENETIC ANALYSIS OF THE PLANT DEFENSE RESPONSEFREDERICK AUSUBEL; Fiscal Year: 2004..elegans esp mutants. Finally, we propose to use genomic and reverse genetic approaches, including DNA microarray analysis, to identify C. elegans and Arabidopsis defense-related genes. ..
- GENETIC ANALYSIS OF THE PLANT DEFENSE RESPONSEFREDERICK AUSUBEL; Fiscal Year: 1993....
- GENETIC ANALYSIS OF THE PLANT DEFENSE RESPONSEFREDERICK AUSUBEL; Fiscal Year: 2000..Finally, in Aim 5, we will clone and characterize selected Arabidopsis defense-related genes using a map-based positional cloning strategy. ..
- Identifying novel anti-infectives by high through-put screening in whole animalsFrederick M Ausubel; Fiscal Year: 2010..Rather than simply preventing bacteria from growing, these new sophisticated drugs will prevent disease by interfering with a microbe's ability to interact with the human body. ..