Rosalyn Adam

Summary

Affiliation: Harvard University
Country: USA

Publications

  1. pmc JunB mediates basal- and TGFβ1-induced smooth muscle cell contractility
    Aruna Ramachandran
    Urological Diseases Research Center, Boston Children s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 8:e53430. 2013
  2. pmc The impact of discrete modes of spinal cord injury on bladder muscle contractility
    Abhishek Seth
    Urological Diseases Research Center, Boston Children s Hospital, Boston, MA 02115, USA
    BMC Urol 13:24. 2013
  3. pmc Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis
    Rosalyn M Adam
    Urological Diseases Research Center, Children s Hospital Boston and Department of Surgery, Harvard Medical School, Boston, MA 02115, USA
    BMC Cell Biol 9:30. 2008
  4. ncbi request reprint GATA-6 mediates human bladder smooth muscle differentiation: involvement of a novel enhancer element in regulating alpha-smooth muscle actin gene expression
    Akihiro Kanematsu
    Urological Diseases Research Center, John F Enders Research Laboratories, Rm 1077, 300 Longwood Ave, Boston, MA 02115, USA
    Am J Physiol Cell Physiol 293:C1093-102. 2007
  5. ncbi request reprint Recent insights into the cell biology of bladder smooth muscle
    Rosalyn M Adam
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, MA 02115, USA
    Nephron Exp Nephrol 102:e1-7. 2006
  6. ncbi request reprint Mechanical stretch is a highly selective regulator of gene expression in human bladder smooth muscle cells
    Rosalyn M Adam
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
    Physiol Genomics 20:36-44. 2004
  7. ncbi request reprint Signaling through PI3K/Akt mediates stretch and PDGF-BB-dependent DNA synthesis in bladder smooth muscle cells
    Rosalyn M Adam
    The Urologic Laboratory, Department of Urology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts, USA
    J Urol 169:2388-93. 2003
  8. ncbi request reprint Involvement of cholesterol-rich lipid rafts in interleukin-6-induced neuroendocrine differentiation of LNCaP prostate cancer cells
    Jayoung Kim
    The Urologic Laboratory, John F Enders Research Laboratories, Room 1161, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    Endocrinology 145:613-9. 2004
  9. ncbi request reprint Activation of the Erk mitogen-activated protein kinase pathway stimulates neuroendocrine differentiation in LNCaP cells independently of cell cycle withdrawal and STAT3 phosphorylation
    Jayoung Kim
    The Urologic Laboratory, Department of Urology, Children s Hospital, and the Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 62:1549-54. 2002
  10. pmc Cholesterol targeting alters lipid raft composition and cell survival in prostate cancer cells and xenografts
    Liyan Zhuang
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    J Clin Invest 115:959-68. 2005

Research Grants

  1. Akt-Regulated Gene Expression in Bladder Smooth Muscle
    Rosalyn Adam; Fiscal Year: 2005
  2. Mechanotransduction in bladder smooth muscle
    Rosalyn Adam; Fiscal Year: 2009
  3. Mechanotransduction in bladder smooth muscle
    Rosalyn M Adam; Fiscal Year: 2010
  4. Mechanotransduction in bladder smooth muscle
    Rosalyn M Adam; Fiscal Year: 2010

Collaborators

Detail Information

Publications34

  1. pmc JunB mediates basal- and TGFβ1-induced smooth muscle cell contractility
    Aruna Ramachandran
    Urological Diseases Research Center, Boston Children s Hospital, Boston, Massachusetts, United States of America
    PLoS ONE 8:e53430. 2013
    ..Together these findings demonstrate a novel function for JunB in regulating visceral smooth muscle cell contractility through effects on both myosin and the actin cytoskeleton...
  2. pmc The impact of discrete modes of spinal cord injury on bladder muscle contractility
    Abhishek Seth
    Urological Diseases Research Center, Boston Children s Hospital, Boston, MA 02115, USA
    BMC Urol 13:24. 2013
    ..The goal of this study is to compare the impact of complete spinal cord transection versus clip compression at thoracic vertebra eight (T8) on bladder muscle contractility...
  3. pmc Rapid preparation of nuclei-depleted detergent-resistant membrane fractions suitable for proteomics analysis
    Rosalyn M Adam
    Urological Diseases Research Center, Children s Hospital Boston and Department of Surgery, Harvard Medical School, Boston, MA 02115, USA
    BMC Cell Biol 9:30. 2008
    ..To address these issues, we have modified a published differential detergent extraction method to enable rapid DRM isolation that minimizes nuclear contamination and yields fractions compatible with mass spectrometry...
  4. ncbi request reprint GATA-6 mediates human bladder smooth muscle differentiation: involvement of a novel enhancer element in regulating alpha-smooth muscle actin gene expression
    Akihiro Kanematsu
    Urological Diseases Research Center, John F Enders Research Laboratories, Rm 1077, 300 Longwood Ave, Boston, MA 02115, USA
    Am J Physiol Cell Physiol 293:C1093-102. 2007
    ..In addition, these findings demonstrate that GATA-6 regulates human alpha-SMA expression via a novel regulatory cis element in the alpha-SMA promoter-enhancer...
  5. ncbi request reprint Recent insights into the cell biology of bladder smooth muscle
    Rosalyn M Adam
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, MA 02115, USA
    Nephron Exp Nephrol 102:e1-7. 2006
    ..In this review, I will summarize our current understanding of cell signaling in bladder smooth muscle and highlight potential targets for drug-based treatment of tissue remodeling in the lower urinary tract...
  6. ncbi request reprint Mechanical stretch is a highly selective regulator of gene expression in human bladder smooth muscle cells
    Rosalyn M Adam
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
    Physiol Genomics 20:36-44. 2004
    ..Moreover, they suggest that mechanical and growth factor signals converge on common transcriptional regulators that include members of the AP-1 family...
  7. ncbi request reprint Signaling through PI3K/Akt mediates stretch and PDGF-BB-dependent DNA synthesis in bladder smooth muscle cells
    Rosalyn M Adam
    The Urologic Laboratory, Department of Urology, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts, USA
    J Urol 169:2388-93. 2003
    ..The effects of growth factor stimulation on pathway activation in bladder SMC were also investigated...
  8. ncbi request reprint Involvement of cholesterol-rich lipid rafts in interleukin-6-induced neuroendocrine differentiation of LNCaP prostate cancer cells
    Jayoung Kim
    The Urologic Laboratory, John F Enders Research Laboratories, Room 1161, Children s Hospital Boston, 300 Longwood Avenue, Boston, MA 02115, USA
    Endocrinology 145:613-9. 2004
    ..Our results also suggest a mechanistic role for membrane cholesterol in cell signaling events relevant to PCa progression...
  9. ncbi request reprint Activation of the Erk mitogen-activated protein kinase pathway stimulates neuroendocrine differentiation in LNCaP cells independently of cell cycle withdrawal and STAT3 phosphorylation
    Jayoung Kim
    The Urologic Laboratory, Department of Urology, Children s Hospital, and the Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 62:1549-54. 2002
    ..They also demonstrate that withdrawal from the cell cycle is not a prerequisite for expression of NE characteristics by PCa...
  10. pmc Cholesterol targeting alters lipid raft composition and cell survival in prostate cancer cells and xenografts
    Liyan Zhuang
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    J Clin Invest 115:959-68. 2005
    ..Our results implicate membrane cholesterol in Akt signaling in both normal and malignant cells and provide evidence that PCa cells can become dependent on a cholesterol-regulated Akt pathway for cell survival...
  11. ncbi request reprint Cholesterol sensitivity of endogenous and myristoylated Akt
    Rosalyn M Adam
    Urological Diseases Research Center, Children s Hospital Boston, Boston, Massachusetts 02115, USA
    Cancer Res 67:6238-46. 2007
    ....
  12. ncbi request reprint An oxidative stress mechanism mediates chelerythrine-induced heparin-binding EGF-like growth factor ectodomain shedding
    Jayoung Kim
    The Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Massachusetts 02115, USA
    J Cell Biochem 94:39-49. 2005
    ..However, JNK signaling was involved in chelerythrine-stimulated apoptosis. Our results suggest that HB-EGF shedding induced by chelerythrine is mediated predominantly via the production of reactive oxygen species...
  13. pmc Heterogeneous nuclear ribonucleoprotein K is a novel regulator of androgen receptor translation
    Nishit K Mukhopadhyay
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, USA
    Cancer Res 69:2210-8. 2009
    ..HnRNP-K is the first protein identified that directly interacts with and regulates the AR translational apparatus...
  14. ncbi request reprint Caveolin-1 interacts with a lipid raft-associated population of fatty acid synthase
    Dolores Di Vizio
    The Urological Diseases Research Center and Department of Surgery, Children s Hospital Boston, Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell Cycle 7:2257-67. 2008
    ..These findings suggest that FASN and Cav-1 physically and functionally interact in PCa cells. They also imply that palmitoylation within this complex is involved in tumor growth and survival...
  15. ncbi request reprint The zinc finger protein ras-responsive element binding protein-1 is a coregulator of the androgen receptor: implications for the role of the Ras pathway in enhancing androgenic signaling in prostate cancer
    Nishit K Mukhopadhyay
    Department of Urology Surgery, Children s Hospital Boston, Harvard Medical School, 300 Longwood Avenue, Boston, Massachusetts 02115, USA
    Mol Endocrinol 21:2056-70. 2007
    ..Collectively, our data for the first time identify RREB-1 as a repressor of AR and further implicate the Ras/MAPK kinase pathway as a likely antagonist of the inhibitory effects of RREB-1 on androgenic signaling...
  16. pmc Transit of hormonal and EGF receptor-dependent signals through cholesterol-rich membranes
    Michael R Freeman
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, United States
    Steroids 72:210-7. 2007
    ..Understanding the manner in which these pathways converge within cholesterol-rich membranes may present new avenues for therapeutic intervention in hormone-dependent cancers...
  17. ncbi request reprint JNK/SAPK and p38 SAPK-2 mediate mechanical stretch-induced apoptosis via caspase-3 and -9 in NRK-52E renal epithelial cells
    Hiep T Nguyen
    Department of Urology, University of California, San Francisco, California, USA
    Nephron Exp Nephrol 102:e49-61. 2006
    ..In this study, we sought to further define the role of JNK and p38 SAPK-2 pathway and caspase activation in stretch-induced apoptosis...
  18. ncbi request reprint Calcium regulates the PI3K-Akt pathway in stretched osteoblasts
    Theodora E Danciu
    Department of Orthopaedic Surgery, John F Enders Research Laboratories, Room 1230, Children s Hospital Boston, 300 Longwood Ave, Boston, MA 02115, USA
    FEBS Lett 536:193-7. 2003
    ....
  19. pmc The pro-apoptotic kinase Mst1 and its caspase cleavage products are direct inhibitors of Akt1
    Bekir Cinar
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA, USA
    EMBO J 26:4523-34. 2007
    ..These results position Mst1/2 within a novel branch of the phosphoinositide 3-kinase/Akt pathway and suggest an important role in cancer progression...
  20. ncbi request reprint Growth and stretch response of human exstrophy bladder smooth muscle cells: molecular evidence of normal intrinsic function
    Anna Orsola
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston and Harvard Medical School, 300 Longwood Avenue, Boston, MA 02115, USA
    BJU Int 95:144-8. 2005
    ..To establish primary cultures of smooth muscle cells (SMC) from human exstrophic bladders (E-SMC), and determine their in vitro growth dynamics and responses to mechanical stretch...
  21. ncbi request reprint Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function
    Rosalyn M Adam
    The Urologic Laboratory, Department of Urology, Children s Hospital Boston and Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Endocrinology 143:4599-608. 2002
    ..These findings also emphasize the potential role of pathways other than the AR axis in acquisition of androgen independence...
  22. ncbi request reprint A quantitative proteomic analysis of growth factor-induced compositional changes in lipid rafts of human smooth muscle cells
    Dawn L MacLellan
    Department of Urology, The Urological Diseases Research Center, Boston, MA, USA
    Proteomics 5:4733-42. 2005
    ..In addition, eight Rho family members were localized to rafts by ICAT analysis. Collectively, these observations suggest a role for lipid rafts in regulation of PDGF-stimulated changes in the cytoskeleton...
  23. pmc An Akt- and Fra-1-dependent pathway mediates platelet-derived growth factor-induced expression of thrombomodulin, a novel regulator of smooth muscle cell migration
    Aruna Ramachandran
    Urological Diseases Research Center, John F Enders Research Laboratories, Room 1077, Children s Hospital Boston, 300 Longwood Ave, Boston, MA 02115, USA
    Am J Pathol 177:119-31. 2010
    ..Moreover, TM induction on bladder injury suggests that it may be a biomarker for pathological smooth muscle remodeling...
  24. ncbi request reprint Inhibition of EGFR signaling abrogates smooth muscle proliferation resulting from sustained distension of the urinary bladder
    Carlos R Estrada
    Urological Diseases Research Center, Department of Urology, Children s Hospital Boston, Boston, MA 02115, USA
    Lab Invest 86:1293-302. 2006
    ..Our results indicate that the EGFR pathway is a physiologically relevant signaling mechanism in hypertrophic bladder disease resulting from mechanical distension and may be amenable to pharmacologic intervention...
  25. pmc Oncosome formation in prostate cancer: association with a region of frequent chromosomal deletion in metastatic disease
    Dolores Di Vizio
    The Urological Diseases Research Center, Children s Hospital Boston, Boston, MA 02115, USA
    Cancer Res 69:5601-9. 2009
    ..They also show that DRF3 is a physiologically relevant protein that seems to regulate this process...
  26. ncbi request reprint Trafficking of nuclear heparin-binding epidermal growth factor-like growth factor into an epidermal growth factor receptor-dependent autocrine loop in response to oxidative stress
    Jayoung Kim
    The Urological Diseases Research Center, Childrens Hospital, Boston, Massachusetts, USA
    Cancer Res 65:8242-9. 2005
    ..These findings show that the nucleus can serve as an intracellular reservoir for a secreted EGFR ligand and, thus, can contribute to an autocrine loop leading to cell proliferation and protection from apoptotic stimuli...
  27. ncbi request reprint Caveolae are negative regulators of transforming growth factor-beta1 signaling in ureteral smooth muscle cells
    Maximilian Stehr
    Department of Urology Urological Diseases Research Center, Children s Hospital Boston, Massachusetts 02115, USA
    J Urol 172:2451-5. 2004
    ..In this study we determined whether growth inhibitory signaling by transforming growth factor-beta1 (TGF-beta1) is also regulated by caveolae in USMC...
  28. ncbi request reprint Tumor cell-associated neuropilin-1 and vascular endothelial growth factor expression as determinants of tumor growth in neuroblastoma
    Karen Marcus
    Department of Radiation Oncology, Children s Hospital and Harvard Medical School, Boston, Massachusetts, USA
    Neuropathology 25:178-87. 2005
    ..Our studies indicate that VEGF and VEGF receptor expression in NBL tumor cells are associated with tumor growth and that angiogenic factors may serve as a biological marker together with already established MYCN amplification...
  29. ncbi request reprint Measurement of plasma levels of vascular endothelial growth factor in prostate cancer patients: relationship with clinical stage, Gleason score, prostate volume, and serum prostate-specific antigen
    José Luis Ferreira Duque
    Brigham and Women s Hospital, Children s Hospital, Harvard Medical School, Boston, MA, USA
    Clinics (Sao Paulo) 61:401-8. 2006
    ..This study focused on circulating levels of vascular endothelial growth factor in patients with prostate cancer compared to a normal population...
  30. ncbi request reprint A nuclear form of the heparin-binding epidermal growth factor-like growth factor precursor is a feature of aggressive transitional cell carcinoma
    Rosalyn M Adam
    Departments of Urology and Surgery, Children s Hospital Boston and Harvard Medical School, Boston, Massachusetts 02115, USA
    Cancer Res 63:484-90. 2003
    ..In addition, our findings suggest that nuclear proHB-EGF may play a role in disease progression in bladder cancer and possibly other cancers...
  31. pmc All-trans retinoic acid directs urothelial specification of murine embryonic stem cells via GATA4/6 signaling mechanisms
    Joshua R Mauney
    Urological Diseases Research Center, Children s Hospital Boston, Boston, Massachusetts, USA
    PLoS ONE 5:e11513. 2010
    ..Collectively, these data suggest that RA mediates ESC specification toward a urothelial lineage via GATA4/6-dependent processes...
  32. ncbi request reprint The decision to undergo DNA or protein synthesis is determined by the degree of mechanical deformation in human bladder muscle cells
    Anna Orsola
    Urologic Laboratory, Department of Urology, Children s Hospital Boston and Department of Surgery, Harvard Medical School, Boston, Massachusetts 02115, USA
    Urology 59:779-83. 2002
    ....
  33. ncbi request reprint Platelet derived growth factor-BB is a potent mitogen for rat ureteral and human bladder smooth muscle cells: dependence on lipid rafts for cell signaling
    Maximilain Stehr
    J Urol 169:1165-70. 2003
    ..In this study we asked whether B, T and U smooth muscle cells (SMC) respond to several SMC growth factors and explored the role of caveolae/lipid raft membrane microdomains in signaling by one of these factors, PDGF-BB...
  34. pmc Induction of smooth muscle cell-like phenotype in marrow-derived cells among regenerating urinary bladder smooth muscle cells
    Akihiro Kanematsu
    Department of Urology, Graduate School of Medicine, Kyoto University, 54 Shogoin Kawaracho, Sakyo, Kyoto, Japan 606 8507
    Am J Pathol 166:565-73. 2005
    ..These findings suggest that marrow cells differentiate into smooth muscle on acellular matrix grafts in response to the environment created by SMC...

Research Grants5

  1. Akt-Regulated Gene Expression in Bladder Smooth Muscle
    Rosalyn Adam; Fiscal Year: 2005
    ..The information obtained from these studies will underpin and inform future studies that will be directed towards elucidation of the mechanism of action of Akt-regulated genes in bladder muscle. ..
  2. Mechanotransduction in bladder smooth muscle
    Rosalyn Adam; Fiscal Year: 2009
    ..We anticipate this analysis will shed new light on fundamental mechanisms underlying bladder muscle physiology and may also provide insight into new treatment strategies. ..
  3. Mechanotransduction in bladder smooth muscle
    Rosalyn M Adam; Fiscal Year: 2010
    ..We anticipate this analysis will shed new light on fundamental mechanisms underlying bladder muscle physiology and may also provide insight into new treatment strategies. ..
  4. Mechanotransduction in bladder smooth muscle
    Rosalyn M Adam; Fiscal Year: 2010
    ..We anticipate this analysis will shed new light on fundamental mechanisms underlying bladder muscle physiology and may also provide insight into new treatment strategies. ..