Research Topics
| MARK PRAUSNITZSummaryAffiliation: Georgia Institute of Technology Country: USA Publications
Research Grants
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Detail Information
Publications
Ultrasound-enhanced drug transport and distribution in the brainYing Liu
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0100, USA
AAPS PharmSciTech 11:1005-17. 2010..Altogether, these studies demonstrate that ultrasound can increase drug permeation in the brain in vitro and in vivo under conditions that did not cause detectable damage...- Microneedle-based vaccinesMark R Prausnitz
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332 0100, USA
Curr Top Microbiol Immunol 333:369-93. 2009.... - Transdermal drug deliveryMark R Prausnitz
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Dr, Atlanta, Georgia 30332 0100, USA
Nat Biotechnol 26:1261-8. 2008..Using these novel second- and third-generation enhancement strategies, transdermal delivery is poised to significantly increase its impact on medicine... 
Coating formulations for microneedlesHarvinder S Gill
Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, GA 30332 0535, USA
Pharm Res 24:1369-80. 2007....- Coated microneedles for transdermal deliveryHarvinder S Gill
Wallace H Coulter Department of Biomedical Engineering at the Georgia Institute of Technology and Emory University, Atlanta, GA 30332 0100, USA
J Control Release 117:227-37. 2007..In conclusion, this study presents a simple, versatile, and controllable method to coat microneedles with proteins, DNA, viruses and microparticles for rapid delivery into the skin... - Transdermal delivery of insulin using microneedles in vivoWijaya Martanto
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
Pharm Res 21:947-52. 2004..CONCLUSIONS: Solid metal microneedles are capable of increasing transdermal insulin delivery and lowering blood glucose levels by as much as 80% in diabetic hairless rats in vivo... - Polymer microneedles for controlled-release drug deliveryJung-Hwan Park
Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
Pharm Res 23:1008-19. 2006..CONCLUSIONS: Biodegradable polymer microneedles can encapsulate drug to provide controlled-release delivery in skin for hours to months... - Insertion of microneedles into skin: measurement and prediction of insertion force and needle fracture forceShawn P Davis
School of Chemical and Biomolecular Engineering, 311 Ferst Drive, Georgia Institute of Technology, Atlanta, GA 30332-0100, USA
J Biomech 37:1155-63. 2004..Together, these results provide the ability to predict insertion and fracture forces, which facilitates rational design of microneedles with robust mechanical properties... - Improved protection against avian influenza H5N1 virus by a single vaccination with virus-like particles in skin using microneedlesJae Min Song
Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA
Antiviral Res 88:244-7. 2010..Thus, this study provides evidence that skin delivery of H5 VLP vaccines using microneedles designed for self-administration induces improved protection compared to conventional intramuscular immunization... - Biodegradable polymer microneedles: fabrication, mechanics and transdermal drug deliveryJung Hwan Park
The Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, GA 30332, USA
J Control Release 104:51-66. 2005..Altogether, these results indicate that biodegradable polymer microneedles can be fabricated with an appropriate geometry and sufficient strength to insert into skin, and thereby dramatically increase transdermal transport of molecules... - Microneedle delivery of H5N1 influenza virus-like particles to the skin induces long-lasting B- and T-cell responses in miceJae Min Song
Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA 30322, USA
Clin Vaccine Immunol 17:1381-9. 2010..Microneedle vaccination in the skin with H5 VLPs represents a promising approach for a self-administered vaccine against viruses with pandemic potential... - Dissolving polymer microneedle patches for influenza vaccinationSean P Sullivan
Wallace H Coulter Department of Biomedical Engineering, Emory University, Atlanta, Georgia, USA
Nat Med 16:915-20. 2010..These results suggest that dissolving microneedle patches can provide a new technology for simpler and safer vaccination with improved immunogenicity that could facilitate increased vaccination coverage... - Current status and future potential of transdermal drug deliveryMark R Prausnitz
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
Nat Rev Drug Discov 3:115-24. 2004.... - Prediction of ultrasound-mediated disruption of cell membranes using machine learning techniques and statistical analysis of acoustic spectraEva K Lee
Department of Radiation Oncology, Emory University School of Medicine, Atlanta, GA 30322, USA
IEEE Trans Biomed Eng 51:82-9. 2004..These rules and the methods used to derive them could be used for real-time feedback about ultrasound's biological effects... - Microneedles for transdermal drug deliveryMark R Prausnitz
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332 0100, USA
Adv Drug Deliv Rev 56:581-7. 2004..Together, these results suggest that microneedles represent a promising technology to deliver therapeutic compounds into the skin for a range of possible applications... - Synergistic enhancement of skin permeability by N-lauroylsarcosine and ethanolYeu Chun Kim
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 0100, USA
Int J Pharm 352:129-38. 2008..We conclude that NLS in aqueous ethanol formulations can dramatically increase skin permeability by a mechanism involving synergistic lipid-fluidization activity in the stratum corneum... - Improved influenza vaccination in the skin using vaccine coated microneedlesYeu Chun Kim
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
Vaccine 27:6932-8. 2009..These findings suggest that vaccination in the skin using a microneedle patch can improve protective immunity, and simplify delivery of influenza and possibly other vaccines... - Formulation and coating of microneedles with inactivated influenza virus to improve vaccine stability and immunogenicityYeu Chun Kim
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
J Control Release 142:187-95. 2010..Overall, these results show that antigen activity loss during microneedle coating can be largely prevented through optimized formulation and that stabilized microneedle patches can be used for effective vaccination... - Biochemical enhancement of transdermal delivery with magainin peptide: modification of electrostatic interactions by changing pHYeu Chun Kim
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 0100, USA
Int J Pharm 362:20-8. 2008..We conclude that magainin increases transdermal delivery by a mechanism involving electrostatic interaction between magainin peptides and drugs... - Microfabricated needles for transdermal delivery of macromolecules and nanoparticles: fabrication methods and transport studiesDevin V McAllister
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta 30332, USA
Proc Natl Acad Sci U S A 100:13755-60. 2003..Hollow microneedles permitted flow of microliter quantities into skin in vivo, including microinjection of insulin to reduce blood glucose levels in diabetic rats... - Mechanism of intracellular delivery by acoustic cavitationRobyn K Schlicher
The Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, Georgia 30332-0100, USA
Ultrasound Med Biol 32:915-24. 2006..In this way, noninvasively focused ultrasound could deliver drugs and genes to targeted tissues, thereby minimizing side effects, lowering drug dosages, and improving efficacy... - Microinfusion using hollow microneedlesWijaya Martanto
School of Chemical and Biomolecular Engineering and Center for Drug Design, Development and Delivery, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
Pharm Res 23:104-13. 2006.... - Mechanism of fluid infusion during microneedle insertion and retractionWijaya Martanto
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332-0100, USA
J Control Release 112:357-61. 2006..Altogether, these results suggest that microneedle insertion to penetrate into the skin followed by microneedle retraction to relieve skin compaction is an effective approach to infuse fluid into the skin in a minimally invasive manner... - Recovery of skin barrier properties after sonication in human subjectsJyoti Gupta
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA
Ultrasound Med Biol 35:1405-8. 2009..Therefore, sonication can increase skin permeability for prolonged periods of time, especially under the effect of occlusion, and has potential to facilitate continuous transdermal drug delivery and diagnostic metabolite extraction... - Precise microinjection into skin using hollow microneedlesPing M Wang
School of Chemical and Biomolecular Engineering, Atlanta, Georgia, USA
J Invest Dermatol 126:1080-7. 2006.... - An electrically active microneedle array for electroporationSeong O Choi
School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
Biomed Microdevices 12:263-73. 2010..This study demonstrates the mechanical and electrical functionalities of the first MEMS-fabricated microneedle array for electroporation, designed for DNA vaccine delivery... - Dissolving microneedles for transdermal drug deliveryJeong W Lee
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332, USA
Biomaterials 29:2113-24. 2008..We conclude that dissolving microneedles can be designed to gently encapsulate molecules, insert into skin, and enable bolus or sustained release delivery... - Electroporation-mediated delivery of molecules to model intestinal epitheliaEsi B Ghartey-Tagoe
The Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, GA 30332, USA
Int J Pharm 270:127-38. 2004..These findings suggest that electroporation may be useful for intracellular delivery into monolayers to study epithelial biology and, possibly, for drug delivery to intestinal epithelium... - Coated microneedles for drug delivery to the eyeJason Jiang
Emory Eye Center, Emory University, Atlanta, Georgia 30322, USA
Invest Ophthalmol Vis Sci 48:4038-43. 2007..To test the hypothesis that coated microneedles can deliver drugs into the eye via intrascleral and intracorneal routes in a minimally invasive manner... - Immunization by vaccine-coated microneedle arrays protects against lethal influenza virus challengeQiyun Zhu
Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
Proc Natl Acad Sci U S A 106:7968-73. 2009..These results show that MNs are highly effective as a simple method of vaccine delivery to elicit protective immune responses against virus infection... - Ultrasonically targeted delivery into endothelial and smooth muscle cells in ex vivo arteriesDaniel M Hallow
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332 0100, USA
J Control Release 118:285-93. 2007..We conclude that significant intracellular uptake of molecules can be targeted into ECs and SMCs by ultrasound-enhanced delivery suggesting possible applications for treatment of cardiovascular diseases and dysfunctions... - Increased permeability of intestinal epithelial monolayers mediated by electroporationEsi B Ghartey-Tagoe
The Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech, Atlanta, GA 30332, USA
J Control Release 103:177-90. 2005..These findings could form the basis for the development of electroporation as a clinical tool to increase intestinal permeability and, thereby, increase the absorption of poorly absorbed drugs... - Intradermal vaccination with influenza virus-like particles by using microneedles induces protection superior to that with intramuscular immunizationFu Shi Quan
Department of Microbiology and Immunology and Emory Vaccine Center, Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Virol 84:7760-9. 2010.... - Tissue electroporation: quantification and analysis of heterogeneous transport in multicellular environmentsPaul J Canatella
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332-0100, USA
Biophys J 86:3260-8. 2004.... 
Fabrication of dissolving polymer microneedles for controlled drug encapsulation and delivery: Bubble and pedestal microneedle designsLeonard Y Chu
Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
J Pharm Sci 99:4228-38. 2010..018 to 0.053 microL per needle. Altogether, these novel microneedle designs provide a new set of tools to fabricate dissolving polymer microneedles with improved control over drug encapsulation, loading, and delivery...- Gene packaging with lipids, peptides and viruses inhibits transfection by electroporation in vitroArlena L Coulberson
School of Chemical Engineering, Georgia Institute of Technology, Atlanta 30332-0100, USA
J Control Release 86:361-70. 2003..The combination of retroviral vectors and electroporation transfected fewer cells than retrovirus alone. We conclude that the combination of electroporation with chemical or viral vectors does not improve gene transfection in vitro... - Enhanced memory responses to seasonal H1N1 influenza vaccination of the skin with the use of vaccine-coated microneedlesYeu Chun Kim
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Emory University School of Medicine, Atlanta, Georgia 30332, USA
J Infect Dis 201:190-8. 2010..Morbidity and mortality due to influenza could be reduced by improved vaccination... - Equilibrium loading of cells with macromolecules by ultrasound: effects of molecular size and acoustic energyHéctor R Guzmán
School of Chemical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0100, USA
J Pharm Sci 91:1693-701. 2002..The results demonstrate that ultrasound provides a means to load viable cells with large numbers of macromolecules, which may be of use for laboratory and possible clinical drug delivery applications... - Intrascleral drug delivery to the eye using hollow microneedlesJason Jiang
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA 30332 0100, USA
Pharm Res 26:395-403. 2009..This study tested the hypothesis that hollow microneedles can infuse solutions containing soluble molecules, nanoparticles, and microparticles into sclera in a minimally invasive manner... - Bioeffects caused by changes in acoustic cavitation bubble density and cell concentration: a unified explanation based on cell-to-bubble ratio and blast radiusHéctor R Guzmán
School of Chemical Engineering, Georgia Institute of Technology, Atlanta, GA 30332 0100, USA
Ultrasound Med Biol 29:1211-22. 2003..Combined, these results suggest that extensive molecular uptake into cells at high viability occurs for low-energy exposure US applied at a high cell-to-bubble ratio... - Selective removal of stratum corneum by microdermabrasion to increase skin permeabilityHarvinder S Gill
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332 0100, USA
Eur J Pharm Sci 38:95-103. 2009..In conclusion, microdermabrasion can selectively remove full-thickness stratum corneum with little damage to deeper tissues and thereby increase skin permeability... - Effect of microneedle design on pain in human volunteersHarvinder S Gill
Center for Drug Design, Development and Delivery, Georgia Institute of Technology, Atlanta, GA 30332 0100, USA
Clin J Pain 24:585-94. 2008.... - Minimally invasive extraction of dermal interstitial fluid for glucose monitoring using microneedlesPing M Wang
School of Chemical and Biomolecular Engineering and Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332-0100, USA
Diabetes Technol Ther 7:131-41. 2005..CONCLUSIONS: These results suggest that microneedle devices can be used to extract ISF for painless glucose monitoring... - Hollow metal microneedles for insulin delivery to diabetic ratsShawn P Davis
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
IEEE Trans Biomed Eng 52:909-15. 2005..Direct measurement of plasma insulin levels showed a peak value of 0.43 ng/ml. Together, these data suggest that microneedles can be fabricated and used for in vivo insulin delivery... - Plasmid DNA and siRNA transfection of intestinal epithelial monolayers by electroporationEsi B Ghartey-Tagoe
The Wallace H. Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Georgia Institute of Technology, Atlanta, GA 30332, USA
Int J Pharm 315:122-33. 2006.... - Measurement and correlation of acoustic cavitation with cellular bioeffectsDaniel M Hallow
School of Chemical and Biomolecular Engineering, Georgia Insitute of Technology, Atlanta, GA 30332-0100, USA
Ultrasound Med Biol 32:1111-22. 2006..These results demonstrate that broadband noise correlates with bioeffects over a broad range of experimental conditions, which suggests a noninvasive feedback method to control ultrasound's bioeffects in real time... - Synergistic effect of ultrasound and PEI on DNA transfection in vitroMangesh C Deshpande
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0100, USA
J Control Release 118:126-35. 2007..Electrophoresis assay showed no damage to DNA or PEI-DNA complexes after sonication. Overall, these results suggest that the combination of ultrasound and PEI can have a synergistic effect to increase DNA transfection... - Mucoadhesive microdiscs engineered for ophthalmic drug delivery: effect of particle geometry and formulation on preocular residence timeYoung Bin Choy
School of Chemical and Biomolecular Engineering and Center for Drug Design, Development and Delivery, Georgia Institute of Technology, Atlanta, Georgia 30332 0100, USA
Invest Ophthalmol Vis Sci 49:4808-15. 2008..To test the hypothesis that mucoadhesive microdiscs formulated in a rapidly dissolving tablet can increase preocular residence time... - Influence of the cell wall on intracellular delivery to algal cells by electroporation and sonicationHarold R Azencott
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA
Ultrasound Med Biol 33:1805-17. 2007.... - Transdermal delivery enhanced by magainin pore-forming peptideYeu Chun Kim
Center for Drug Design, Development and Delivery and the Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, United States
J Control Release 122:375-83. 2007..This study also presents the novel concept of using a first chemical enhancer to increase penetration of a second chemical enhancer into the skin to synergistically increase skin permeability to a model drug... - Quantification of optison bubble size and lifetime during sonication dominant role of secondary cavitation bubbles causing acoustic bioeffectsPavel P Kamaev
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, Georgia 30332-0100, USA
J Acoust Soc Am 115:1818-25. 2004..Because Optison solutions presonicated to destroy all detectable bubbles also caused significant bioeffects, the indirect mechanism involving secondary cavitation bubbles is more likely... - Model of transient drug diffusion across corneaWensheng Zhang
Department of Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA
J Control Release 99:241-58. 2004..Because of its mechanistic basis, this model will be useful to predict drug transport kinetics and bioavailability for new compounds and in diseased eyes... - Microneedles permit transdermal delivery of a skin-impermeant medication to humansDaniel P Wermeling
Department of Pharmacy Practice, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA
Proc Natl Acad Sci U S A 105:2058-63. 2008..These findings set the stage for future human studies of skin-impermeant medications and biopharmaceuticals for clinical applications... - Tapered conical polymer microneedles fabricated using an integrated lens technique for transdermal drug deliveryJung Hwan Park
Department of BioNano Technology, Kyungwon University, Gachon BioNano Research Institute, Gyeonggi do 461 701, Korea
IEEE Trans Biomed Eng 54:903-13. 2007..quot;Long" polymer microneedles were also demonstrated in human subjects to insert deeply without failure... - Future directions for therapeutic ultrasoundPaul Campbell
Ultrasound Med Biol 33:657. 2007 - A peptide chaperone for transdermal drug deliveryMark R Prausnitz
Nat Biotechnol 24:416-7. 2006 - Flux across [corrected] microneedle-treated skin is increased by increasing charge of naltrexone and naltrexol in vitroStan L Banks
Department of Pharmaceutical Sciences, University of Kentucky College of Pharmacy, Lexington, Kentucky 40536 0082, USA
Pharm Res 25:1677-85. 2008..In a second set of experiments, permeability of the major active metabolite 6-beta-naltrexol base (NTXOL) in the primarily unionized (unprotonated) form at pH 8.5 was compared to the ionized form (pH 4.5)... - The effect of heat on skin permeabilityJung Hwan Park
Department of BioNano Technology and Gachon BioNano Research Institute, Kyungwon University, Seongnam, Geonggi do 461 701, Republic of Korea
Int J Pharm 359:94-103. 2008..We conclude that heating the skin with short, high temperature pulses can increase skin permeability by orders of magnitude due to structural disruption and removal of stratum corneum...
Research Grants
- Microneedle-based immunization against pandemic influenzaMARK PRAUSNITZ; Fiscal Year: 2009..Relevance: During an influenza pandemic, microneedle-based vaccination should save lives by rapidly immunizing millions of people using a self-administered, dose-sparing, transdermal patch ..
- Microneedle-based immunization against pandemic influenzaMARK PRAUSNITZ; Fiscal Year: 2009..Relevance: During an influenza pandemic, microneedle-based vaccination should save lives by rapidly immunizing millions of people using a self-administered, dose-sparing, transdermal patch ..
- Microneedle-based immunization against pandemic influenzaMARK PRAUSNITZ; Fiscal Year: 2007..Relevance: During an influenza pandemic, microneedle-based vaccination should save lives by rapidly immunizing millions of people using a self-administered, dose-sparing, transdermal patch ..
- Mechanisms of Ultrasound-Mediated BioeffectsMARK PRAUSNITZ; Fiscal Year: 2006..During the final year, mechanistic findings will be used to design and test ultrasound protocols that optimize molecular uptake and cell viability. ..
- MICROFABRICATED MICRONEEDLES FOR DRUG DELIVERYMARK PRAUSNITZ; Fiscal Year: 2003..g., proteins, DNA) over hours to days. ..
- Microneedle-based immunization against pandemic influenzaMark R Prausnitz; Fiscal Year: 2010..Relevance: During an influenza pandemic, microneedle-based vaccination should save lives by rapidly immunizing millions of people using a self-administered, dose-sparing, transdermal patch ..