J C Powers

Summary

Affiliation: Georgia Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Cutaneous protease activity in the mouse ear vesicant model
    J C Powers
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30332 0400, USA
    J Appl Toxicol 20:S177-82. 2000
  2. ncbi request reprint Irreversible inhibitors of serine, cysteine, and threonine proteases
    James C Powers
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Chem Rev 102:4639-750. 2002
  3. ncbi request reprint Granzymes (lymphocyte serine proteases): characterization with natural and synthetic substrates and inhibitors
    C M Kam
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Biochim Biophys Acta 1477:307-23. 2000
  4. ncbi request reprint Inhibition of arginine gingipains (RgpB and HRgpA) with benzamidine inhibitors: zinc increases inhibitory potency
    Joel A Krauser
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30093 0400, USA
    Biol Chem 383:1193-8. 2002
  5. pmc Peptidyl alpha-ketoamides with nucleobases, methylpiperazine, and dimethylaminoalkyl substituents as calpain inhibitors
    Asli Ovat
    School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    J Med Chem 53:6326-36. 2010
  6. ncbi request reprint Peptidyl allyl sulfones: a new class of inhibitors for clan CA cysteine proteases
    Marion G Götz
    School of Chemistry and Biochemistry and the Petit Institute of Bioscience and Bioengineering, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Bioorg Med Chem 12:5203-11. 2004
  7. ncbi request reprint Subsite specificities of granzyme M: a study of inhibitors and newly synthesized thiobenzyl ester substrates
    Brian J Rukamp
    The School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Arch Biochem Biophys 422:9-22. 2004
  8. ncbi request reprint Aza-peptide epoxides: a new class of inhibitors selective for clan CD cysteine proteases
    Juliana L Asgian
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30332 0400, USA
    J Med Chem 45:4958-60. 2002
  9. ncbi request reprint Aza-peptide epoxides: potent and selective inhibitors of Schistosoma mansoni and pig kidney legumains (asparaginyl endopeptidases)
    Karen Ellis James
    School of Chemistry and Biochemistry, and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Biol Chem 384:1613-8. 2003
  10. doi request reprint Aza-peptidyl Michael acceptors. A new class of potent and selective inhibitors of asparaginyl endopeptidases (legumains) from evolutionarily diverse pathogens
    Marion G Götz
    School of Chemistry and Biochemistry and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    J Med Chem 51:2816-32. 2008

Research Grants

  1. CASPASE INHIBITORS
    James Powers; Fiscal Year: 2003

Collaborators

  • James McKerrow
  • Takahisa Imamura
  • J Potempa
  • Alan J Barrett
  • J Travis
  • K M Ricketts
  • R P Casillas
  • J J Buccafusco
  • Karen Ellis James
  • Zhao Zhao Li
  • Marion G Götz
  • Juliana L Asgian
  • Elizabeth Hansell
  • Conor R Caffrey
  • Asli Ovat
  • Ozlem Dogan Ekici
  • Ting Wai Lee
  • Guy S Salvesen
  • Jowita Mikolajczyk
  • Jan Dvorak
  • Rajkumar Ganesan
  • Brian J Rukamp
  • Petr Kopacek
  • Daniel Sojka
  • Jin Qian
  • Shirin Arastu-Kapur
  • Michael N G James
  • Maia M Cherney
  • Lindsay D Eltis
  • Jie Liu
  • Stjepan Jelakovic
  • Markus G Grutter
  • Amy J Campbell
  • Rasmus Kragh Jakobsen
  • Chih Min Kam
  • Wendy Carter
  • Joel A Krauser
  • Tinh V Tran
  • Seneshaw A Asress
  • Facundo M Fernandez
  • Christina Y Hampton
  • C M Kam
  • Jonathan D Glass
  • Michelle Brouner
  • Fanuel Muindi
  • Crystal Fagan
  • Carolyn I Phillips
  • Peter L Davies
  • Sharon Yeoh
  • Elizabeth L Ponder
  • Munira Grainger
  • Zhaozhao Li
  • Dominic Cuerrier
  • Marko Fonovic
  • Robert L Campbell
  • Matthew Bogyo
  • Ursa Pecar Fonović
  • Amritha Seshaadri
  • Fang Yuan
  • Carly Huitema
  • Shin Ono
  • Robert DeLotto
  • Mark J Smyth
  • Janice M Kelly
  • John R Rubin
  • Brad W Bolton
  • Sudah Natarajan
  • Karen A Ellis
  • Dorothy Hudig
  • D Hudig

Detail Information

Publications22

  1. ncbi request reprint Cutaneous protease activity in the mouse ear vesicant model
    J C Powers
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30332 0400, USA
    J Appl Toxicol 20:S177-82. 2000
    ....
  2. ncbi request reprint Irreversible inhibitors of serine, cysteine, and threonine proteases
    James C Powers
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    Chem Rev 102:4639-750. 2002
  3. ncbi request reprint Granzymes (lymphocyte serine proteases): characterization with natural and synthetic substrates and inhibitors
    C M Kam
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Biochim Biophys Acta 1477:307-23. 2000
    ..Studies of substrate and inhibitor kinetics are providing valuable information to identify the most likely natural granzyme substrates and provide tools for the study of key reactions in the cytolytic mechanism...
  4. ncbi request reprint Inhibition of arginine gingipains (RgpB and HRgpA) with benzamidine inhibitors: zinc increases inhibitory potency
    Joel A Krauser
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30093 0400, USA
    Biol Chem 383:1193-8. 2002
    ..In summary, we have discovered a new series of effective inhibitors for the gingipains and found a novel way to increase inhibitor potency with the HRgpA and RgpB gingipains using zinc...
  5. pmc Peptidyl alpha-ketoamides with nucleobases, methylpiperazine, and dimethylaminoalkyl substituents as calpain inhibitors
    Asli Ovat
    School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    J Med Chem 53:6326-36. 2010
    ..These inhibitors have good potential to be used in the treatment of neurodegenerative diseases...
  6. ncbi request reprint Peptidyl allyl sulfones: a new class of inhibitors for clan CA cysteine proteases
    Marion G Götz
    School of Chemistry and Biochemistry and the Petit Institute of Bioscience and Bioengineering, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Bioorg Med Chem 12:5203-11. 2004
    ..It was observed that the stereochemistry of the vinyl sulfone precursor played a role in the potency of the dipeptidyl allyl sulfone inhibitor...
  7. ncbi request reprint Subsite specificities of granzyme M: a study of inhibitors and newly synthesized thiobenzyl ester substrates
    Brian J Rukamp
    The School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Arch Biochem Biophys 422:9-22. 2004
    ..The observations in this paper will be valuable in development of new potent inhibitors for granzyme M as well as assist in determining the biological function of the enzyme...
  8. ncbi request reprint Aza-peptide epoxides: a new class of inhibitors selective for clan CD cysteine proteases
    Juliana L Asgian
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta 30332 0400, USA
    J Med Chem 45:4958-60. 2002
    ..The aza-Asn derivatives are effective legumain inhibitors, while the aza-Asp epoxides were specific for caspases. The inhibitors have little or no inhibition with other proteases such as chymotrypsin, papain, or cathepsin B...
  9. ncbi request reprint Aza-peptide epoxides: potent and selective inhibitors of Schistosoma mansoni and pig kidney legumains (asparaginyl endopeptidases)
    Karen Ellis James
    School of Chemistry and Biochemistry, and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Biol Chem 384:1613-8. 2003
    ..The inhibitors have little or no inhibitory activity with other proteases such as caspases, chymotrypsin, papain, cathepsin B, granzyme B, and various aspartyl proteases...
  10. doi request reprint Aza-peptidyl Michael acceptors. A new class of potent and selective inhibitors of asparaginyl endopeptidases (legumains) from evolutionarily diverse pathogens
    Marion G Götz
    School of Chemistry and Biochemistry and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    J Med Chem 51:2816-32. 2008
    ..The preferred P1' residues have aromatic substituents. Aza-asparaginyl Michael acceptors react with thiols, which provides insight into the mechanism of their inhibition of asparaginyl endopeptidases...
  11. ncbi request reprint Design, synthesis, and evaluation of aza-peptide Michael acceptors as selective and potent inhibitors of caspases-2, -3, -6, -7, -8, -9, and -10
    Ozlem Dogan Ekici
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    J Med Chem 49:5728-49. 2006
    ....
  12. ncbi request reprint Aza-peptide Michael acceptors: a new class of inhibitors specific for caspases and other clan CD cysteine proteases
    Ozlem Dogan Ekici
    School of Chemistry and Biochemistry and the Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    J Med Chem 47:1889-92. 2004
    ..Aza-Lys and aza-Orn derivatives were potent inhibitors of gingipain K and clostripain. Aza-peptide Michael acceptors showed no cross reactivity toward papain, cathepsin B, and calpain...
  13. ncbi request reprint Design, synthesis, and evaluation of aza-peptide epoxides as selective and potent inhibitors of caspases-1, -3, -6, and -8
    Karen Ellis James
    School of Chemistry and Biochemistry and the Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    J Med Chem 47:1553-74. 2004
    ....
  14. ncbi request reprint Dipeptidyl peptidase I: importance of progranzyme activation sequences, other dipeptide sequences, and the N-terminal amino group of synthetic substrates for enzyme activity
    Tinh V Tran
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332 0400, USA
    Arch Biochem Biophys 403:160-70. 2002
    ..The results reported in this paper should be useful in the design of better DPPI inhibitors to block granzyme maturation and granzyme-dependent apoptosis...
  15. doi request reprint Aza-peptidyl Michael acceptor and epoxide inhibitors--potent and selective inhibitors of Schistosoma mansoni and Ixodes ricinus legumains (asparaginyl endopeptidases)
    Asli Ovat
    School of Chemistry and Biochemistry, Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0400, USA
    J Med Chem 52:7192-210. 2009
    ..Given the central functions of these enzymes in both parasites, the data presented here may facilitate the eventual design of selective antiparasitic drugs...
  16. ncbi request reprint Crystal structures of the main peptidase from the SARS coronavirus inhibited by a substrate-like aza-peptide epoxide
    Ting Wai Lee
    Canadian Institute of Health Research Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alta, Canada T6G 2H7
    J Mol Biol 353:1137-51. 2005
    ..5, contrary to the previously determined crystal structures of unbound M(pro) in the space group P2(1)...
  17. pmc Cocrystal structures of primed side-extending alpha-ketoamide inhibitors reveal novel calpain-inhibitor aromatic interactions
    Jin Qian
    Department of Biochemistry, Queen s University, Kingston, Ontario, K7L 3N6, Canada
    J Med Chem 51:5264-70. 2008
    ..Moreover, stacking orients the adenine such that it can be used as a scaffold for designing novel primed-side address regions, which could be incorporated into future inhibitors to enhance their calpain specificity...
  18. doi request reprint Identification of proteases that regulate erythrocyte rupture by the malaria parasite Plasmodium falciparum
    Shirin Arastu-Kapur
    Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305, USA
    Nat Chem Biol 4:203-13. 2008
    ..These results suggest that two mechanistically distinct proteases function to regulate processing of downstream substrates required for efficient release of parasites from host red blood cells...
  19. ncbi request reprint MHP-133, a drug with multiple CNS targets: potential for neuroprotection and enhanced cognition
    Jerry J Buccafusco
    Alzheimer s Research Center, Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, GA 30912, USA
    Neurochem Res 32:1224-37. 2007
    ..The results of this study are consistent with the potential use of MHP-133 in the treatment of neurodegenerative disorders such as Alzheimer's disease...
  20. ncbi request reprint Crystal structures reveal an induced-fit binding of a substrate-like Aza-peptide epoxide to SARS coronavirus main peptidase
    Ting Wai Lee
    Group in Protein Structure and Function, Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada
    J Mol Biol 366:916-32. 2007
    ..The structural data further suggest that the binding of APE to M(pro) follows an induced-fit model. The substrate likely also binds in an induced-fit manner in a process that may help drive the catalytic cycle...
  21. ncbi request reprint Exploring the S4 and S1 prime subsite specificities in caspase-3 with aza-peptide epoxide inhibitors
    Rajkumar Ganesan
    Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH 8057 Zurich, Switzerland
    Biochemistry 45:9059-67. 2006
    ....
  22. ncbi request reprint Fluorescently labeled inhibitors detect localized serine protease activities in Drosophila melanogaster pole cells, embryos, and ovarian egg chambers
    Rasmus Kragh Jakobsen
    Department of Genetics, Institute of Molecular Biology, University of Copenhagen, Øster Farimagsgade 2A, 1353 Copenhagen K, Denmark
    Histochem Cell Biol 123:51-60. 2005
    ..Our results suggest that this technique holds promise to identify new spatially restricted activities in adult Drosophila tissues and developing embryos...

Research Grants4

  1. CASPASE INHIBITORS
    James Powers; Fiscal Year: 2003
    ..This research should provide information of the active site structure of the various caspases and lead to the design of better drugs. ..