Manu O Platt
Affiliation: Georgia Institute of Technology
- Patient specific proteolytic activity of monocyte-derived macrophages and osteoclasts predicted with temporal kinase activation states during differentiationKeon Young Park
Department of Biomedical Engineering and Parker H Petit Institute of Bioengineering and Biosciences, Georgia Institute of Technology and Emory University, 315 Ferst Drive, Atlanta, GA 30332, USA
Integr Biol (Camb) 4:1459-69. 2012..Such analyses may provide beneficial tools for personalized kinase and protease inhibitor therapies for tissue destructive diseases...
- T-cell phosphokinome as a fingerprint of effective graft versus leukemiaManu O Platt
Wallace H Coulter Department of Biomedical Engineering and Institute for Bioengineering and Biosciences, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Front Biosci (Elite Ed) 4:721-33. 2012..Higher throughput methods to characterize these signals and different model systems will be discussed, along with benefits and challenges of using the T cell phosphokinome as a fingerprint to predict GvHD and GvL...
- Multiplex zymography captures stage-specific activity profiles of cathepsins K, L, and S in human breast, lung, and cervical cancerBinbin Chen
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, GA 30332, Atlanta, USA
J Transl Med 9:109. 2011..Here, we have developed multiplex cathepsin zymography to profile cathepsins K, L, and S activity in 10 μg human breast, lung, and cervical tumors by exploiting unique electrophoretic mobility and renaturation properties...
- Sickle cell biomechanicsGilda A Barabino
The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
Annu Rev Biomed Eng 12:345-67. 2010..This review discusses the characterization of the biomechanical properties of sickle RBCs and sickle blood as well as their implications toward a better understanding of the pathophysiology of the disease...
- Manipulating substrate and pH in zymography protocols selectively distinguishes cathepsins K, L, S, and V activity in cells and tissuesCatera L Wilder
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, 30332, USA
Arch Biochem Biophys 516:52-7. 2011....
- Expression of cathepsin K is regulated by shear stress in cultured endothelial cells and is increased in endothelium in human atherosclerosisManu O Platt
Wallace H Coulter Dept of Biomedical Engineering at Georgia Tech and Emory Univ, 308D WMB, Atlanta, GA 30322, USA
Am J Physiol Heart Circ Physiol 292:H1479-86. 2007..These findings suggest that cathepsin K is a mechanosensitive, extracellular matrix protease that, in turn, may be involved in arterial wall remodeling and atherosclerosis...