Christine K Payne

Summary

Affiliation: Georgia Institute of Technology
Country: USA

Publications

  1. pmc Lysosome transport as a function of lysosome diameter
    Debjyoti Bandyopadhyay
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America
    PLoS ONE 9:e86847. 2014
  2. pmc Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran
    William H Humphries
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States of America
    PLoS ONE 6:e26626. 2011
  3. ncbi request reprint Imaging gene delivery with fluorescence microscopy
    Christine K Payne
    Georgia Institute of Technology, School of Chemistry and Biochemistry, Atlanta, GA 30332 0400, USA
    Nanomedicine (Lond) 2:847-60. 2007
  4. pmc Imaging lysosomal enzyme activity in live cells using self-quenched substrates
    William H Humphries
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Anal Biochem 424:178-83. 2012
  5. doi request reprint Cellular binding of nanoparticles in the presence of serum proteins
    Gerard W Doorley
    School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    Chem Commun (Camb) 47:466-8. 2011
  6. doi request reprint Imaging intracellular quantum dots: fluorescence microscopy and transmission electron microscopy
    Craig J Szymanski
    Georgia Institute of Technology, Atlanta, GA, USA
    Methods Mol Biol 1026:21-33. 2013
  7. doi request reprint Nanoparticles act as protein carriers during cellular internalization
    Gerard W Doorley
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    Chem Commun (Camb) 48:2961-3. 2012
  8. ncbi request reprint Single particle tracking as a method to resolve differences in highly colocalized proteins
    Craig J Szymanski
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    Analyst 136:3527-33. 2011
  9. doi request reprint Intracellular degradation of low-density lipoprotein probed with two-color fluorescence microscopy
    William H Humphries
    Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, 30332, USA
    Integr Biol (Camb) 2:536-44. 2010
  10. pmc Nanoparticle surface charge mediates the cellular receptors used by protein-nanoparticle complexes
    Candace C Fleischer
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    J Phys Chem B 116:8901-7. 2012

Research Grants

Collaborators

  • Ye Li
  • Niren Murthy
  • William H Humphries
  • Craig J Szymanski
  • Gerard W Doorley
  • Debjyoti Bandyopadhyay
  • Edwin H Shin
  • Khalilah G Reddie
  • Candace C Fleischer
  • Nicole C Fay
  • Gaelen T Hess
  • Oliver Hayden
  • Austin Cyphersmith
  • Jairo A Zapata
  • Y Joseph Kim
  • Elizabeth R Wright
  • Joshua L Liu
  • Umesh Kumar
  • Hong Yi
  • Xianren Zhang
  • Hursh V Sureka
  • Melissa L Kemp
  • Charlo P Bain

Detail Information

Publications14

  1. pmc Lysosome transport as a function of lysosome diameter
    Debjyoti Bandyopadhyay
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, United States of America
    PLoS ONE 9:e86847. 2014
    ..We find, as expected, the diffusive component of intracellular transport is decreased proportional to the increased lysosome diameter. Active transport of the enlarged lysosomes is not affected by the increased lysosome diameter. ..
  2. pmc Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran
    William H Humphries
    School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, United States of America
    PLoS ONE 6:e26626. 2011
    ..We find no correlation with M6PR but do find that Rab7-vesicles undergo significantly fewer fusion events than LAMP1- or Rab7/LAMP1-vesicles suggesting that the distribution of fluid phase cargo is driven by vesicle dynamics...
  3. ncbi request reprint Imaging gene delivery with fluorescence microscopy
    Christine K Payne
    Georgia Institute of Technology, School of Chemistry and Biochemistry, Atlanta, GA 30332 0400, USA
    Nanomedicine (Lond) 2:847-60. 2007
    ..The focus of this review is the use of synthetic gene-delivery vectors, especially polyethylenimine, and the live-cell imaging and single-particle tracking techniques that reveal the intracellular dynamics of the gene-delivery process...
  4. pmc Imaging lysosomal enzyme activity in live cells using self-quenched substrates
    William H Humphries
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Anal Biochem 424:178-83. 2012
    ..Using live cell imaging and single particle tracking, we find that the degradation of bovine serum albumin occurs in an endo-lysosomal vesicle that is positive for LAMP1...
  5. doi request reprint Cellular binding of nanoparticles in the presence of serum proteins
    Gerard W Doorley
    School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    Chem Commun (Camb) 47:466-8. 2011
    ..Cationic nanoparticles associate with serum proteins in solution and bind to the cell surface as a single anionic complex. Displacement of serum proteins from the nanoparticles was found to be protein dependent...
  6. doi request reprint Imaging intracellular quantum dots: fluorescence microscopy and transmission electron microscopy
    Craig J Szymanski
    Georgia Institute of Technology, Atlanta, GA, USA
    Methods Mol Biol 1026:21-33. 2013
    ..To overcome the difficulties associated with using TEM to image individual QDs in cells, we have utilized a silver enhancement method that significantly improves the contrast of QDs in TEM images...
  7. doi request reprint Nanoparticles act as protein carriers during cellular internalization
    Gerard W Doorley
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    Chem Commun (Camb) 48:2961-3. 2012
    ..This study demonstrates the importance of nanoparticle-protein interactions in cellular applications...
  8. ncbi request reprint Single particle tracking as a method to resolve differences in highly colocalized proteins
    Craig J Szymanski
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    Analyst 136:3527-33. 2011
    ..This research demonstrates the use of single particle tracking as a tool to resolve functional differences in highly colocalized proteins in intact live cells...
  9. doi request reprint Intracellular degradation of low-density lipoprotein probed with two-color fluorescence microscopy
    William H Humphries
    Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, 30332, USA
    Integr Biol (Camb) 2:536-44. 2010
    ..More generally, this research provides a model for the intracellular degradation of extracellular cargo and a method for its study in living cells...
  10. pmc Nanoparticle surface charge mediates the cellular receptors used by protein-nanoparticle complexes
    Candace C Fleischer
    School of Chemistry and Biochemistry and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    J Phys Chem B 116:8901-7. 2012
    ..As nanoparticles become increasingly important for in vivo applications, we expect these results will inform the design of nanoparticles with improved cellular binding...
  11. pmc Membrane potential mediates the cellular binding of nanoparticles
    Edwin H Shin
    School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332, USA
    Nanoscale 5:5879-86. 2013
    ....
  12. pmc Fluorescent coumarin thiols measure biological redox couples
    Khalilah G Reddie
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30032, USA
    Org Lett 14:680-3. 2012
    ..3-HTC reacts reversibly with thiols and disulfides enabling its use to measure dynamic GSH/GSSH ratios in vitro as well as to monitor the reversible redox status of whole cell lysates...
  13. pmc Cellular binding, motion, and internalization of synthetic gene delivery polymers
    Gaelen T Hess
    Biophysics Program, Harvard University, Cambridge, MA 02138, USA
    Biochim Biophys Acta 1773:1583-8. 2007
    ..Understanding the interaction of polyarginine and polyethylenimine with the plasma membrane may assist in designing more efficient gene delivery systems...
  14. ncbi request reprint Nanophotonic light sources for fluorescence spectroscopy and cellular imaging
    Oliver Hayden
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA
    Angew Chem Int Ed Engl 44:1395-8. 2005

Research Grants4

  1. Single-Molecule Imaging Studies of the Polyomaviruses
    Christine Payne; Fiscal Year: 2006
    ..Comparisons to SV40 and influenza will provide a broad understanding of viral infection that will be essential in the development of antiviral drugs. ..
  2. Polyomavirus transport: Vesicles, motor proteins, and endocytosis.
    Christine Payne; Fiscal Year: 2007
    ..Overall, this research is expected to contribute significantly to our understanding of three interrelated fields; viral infection, imaging technology, and intracellular transport. ..
  3. Intracellular delivery and targeting of nanoparticles
    Christine Payne; Fiscal Year: 2009
    ..These studies will use a modular scheme for functionalization that can be extended to a wide variety of nanoparticles for broad impact in many different disciplines including cancer therapy, gene delivery, and cellular imaging. ..