Research Topics
Species | Robert M NeremSummaryAffiliation: Georgia Institute of Technology Country: USA Publications
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Publications
Regenerative medicine: the emergence of an industryRobert M Nerem
Parker H Petit Institute for Bioengineering and Bioscience, Georgia Tech Emory Center for Regenerative Medicine, Georgia Institute of Technology, 315 Ferst Drive, NW, Atlanta, GA 30332 0363, USA
J R Soc Interface 7:S771-5. 2010..Thus, there is the indication that this industry, and the related technology, may still achieve its potential and address the needs of millions of patients worldwide, in particular those with needs that currently are unmet...
Cell-based therapies: from basic biology to replacement, repair, and regenerationRobert M Nerem
Georgia Institute of Technology, Atlanta, GA 30332 0363, USA
Biomaterials 28:5074-7. 2007..Long-term clinical therapies and treatments must move beyond the replacement of tissues and organs to the harnessing of the intrinsic repair and regenerative potential of the human body...- Tissue engineering a blood vessel substitute: the role of biomechanicsR M Nerem
Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta 30332 0363, USA
Yonsei Med J 41:735-9. 2000..Only if an engineered blood vessel substitute possesses all of these functional characteristics, can one say that the functionality exhibited by a native vessel is being mimicked... - Vascular tissue engineeringR M Nerem
Georgia Tech Emory Center for the Engineering of Living Tissues, Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332 0363, USA
Annu Rev Biomed Eng 3:225-43. 2001..Specific examples are discussed to illustrate both the benefits and shortcomings of each methodology, as well as to underline common themes. Finally, a brief perspective on challenges for the future is presented... - The tissue engineering of blood vessels and the heartRobert M Nerem
Georgia Tech Emory Center for the Engineering of Living Tissues, Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA
Am J Transplant 4:36-42. 2004..Even though the progress has been considerable and there are even some approaches that have entered clinical trials, we are still a ways off from having FDA-approved replacements and/or repair strategies... - Role of mechanics in vascular tissue engineeringRobert M Nerem
Petit Institute for Bioengineering and Bioscience, 315 Ferst Drive, Georgia Institute of Technology, Atlanta, GA 30332 0363, USA
Biorheology 40:281-7. 2003..Thus, the proper incorporation of biomechanics into the design of a vascular substitute is critical to achieving success... - Tissue engineering of the vascular systemR M Nerem
Georgia Tech/Emory Center for the Engineering of Living Tissues, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta 30332-0363, USA
Vox Sang 87:158-60. 2004 - Incorporation of intact elastin scaffolds in tissue-engineered collagen-based vascular graftsJoseph D Berglund
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
Tissue Eng 10:1526-35. 2004..Future challenges include stimulating appropriate reorganization or synthesis of the collagen matrix to provide the necessary strength and viscoelastic properties for implantation... - Fluid shear stress alters the hemostatic properties of endothelial outgrowth cellsAnn E Ensley
Parker H Petit Institute for Bioengineering and Bioscience and Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA
Tissue Eng Part A 18:127-36. 2012..Accordingly, manipulation of EOC phenotype by mechanical forces may be important for the development of thrombo-resistant surfaces on engineered vascular implants... 
Human mesenchymal stem cells form multicellular structures in response to applied cyclic strainAdele M Doyle
Parker H Petit Institute for Biomedical Engineering and Biosciences, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332 0363, USA
Ann Biomed Eng 37:783-93. 2009..This system can thus be used to study the mechanoresponse over hours to days of MSCs exposed to applied cyclic strain in the context of cell-cell and cell-matrix interactions...- Embryonic stem cell-derived endothelial cells may lack complete functional maturation in vitroKara E McCloskey
The Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA
J Vasc Res 43:411-21. 2006..These results indicate that ESC-derived EC share some key characteristics of 'mature' EC, while retaining markers of alternate phenotypes including immature endothelium... - Phenotype modulation in vascular tissue engineering using biochemical and mechanical stimulationJan P Stegemann
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA
Ann Biomed Eng 31:391-402. 2003.... - Valvular endothelial cells regulate the phenotype of interstitial cells in co-culture: effects of steady shear stressJonathan T Butcher
Petit Institute for Bioengineering and Bioscience, Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, 30332, USA
Tissue Eng 12:905-15. 2006..Additionally, we show that tissue-engineered models can be used to discover and understand complex biomechanical relationships between cells that interact in vivo... - Mechanical, biochemical, and extracellular matrix effects on vascular smooth muscle cell phenotypeJan P Stegemann
Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
J Appl Physiol 98:2321-7. 2005..Future improvements in creating more complex in vitro culture environments will lead to a better understanding of VSMC biology, new treatments for vascular disease, as well as improved blood vessel substitutes... - Use of embryonic stem cell-derived endothelial cells as a cell source to generate vessel structures in vitroKara E McCloskey
The Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
Tissue Eng 11:497-505. 2005.... - In vitro derivation and expansion of endothelial cells from embryonic stem cellsKara E McCloskey
School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, USA
Methods Mol Biol 330:287-301. 2006..Using this in vitro derivation procedure, millions of cells at various stages of differentiation may be obtained and expanded up to 25 population doublings... - Viscoelastic testing methodologies for tissue engineered blood vesselsJoseph D Berglund
School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
J Biomech Eng 127:1176-84. 2005..These findings emphasize the importance of viscoelastic characterization when evaluating the mechanical performance of TEBVs. Limits of testing methods and modeling systems are presented and discussed... - Equibiaxial strain stimulates fibroblastic phenotype shift in smooth muscle cells in an engineered tissue model of the aortic wallJonathan T Butcher
Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA
Biomaterials 27:5252-8. 2006..We conclude that this system is effective in stimulating cells with cyclic equibiaxial strain in 3D cultures, and can be applied to a variety of biomaterial and tissue engineering applications... - Properties of engineered vascular constructs made from collagen, fibrin, and collagen-fibrin mixturesChristopher L Cummings
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
Biomaterials 25:3699-706. 2004..These results demonstrate that the properties of engineered vascular tissues can be modulated by the combination of selected extracellular matrix components, and the application of mechanical stimulation... - Altered response of vascular smooth muscle cells to exogenous biochemical stimulation in two- and three-dimensional cultureJan P Stegemann
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA
Exp Cell Res 283:146-55. 2003..These findings have implications for the study of vascular biology in vitro, as well as for the development of engineered vascular tissues... - Discovery of shear- and side-specific mRNAs and miRNAs in human aortic valvular endothelial cellsCasey J Holliday
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA
Am J Physiol Heart Circ Physiol 301:H856-67. 2011..Using miRWalk and several filtering steps, we identified shear-sensitive mRNAs potentially targeted by shear-sensitive miRNAs. These genes and signaling pathways could act as therapeutic targets of AV disease... - Porcine aortic valve interstitial cells in three-dimensional culture: comparison of phenotype with aortic smooth muscle cellsJonathan T Butcher
Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332, USA
J Heart Valve Dis 13:478-85; discussion 485-6. 2004..Other potential cell sources for heart valve tissue engineering may not be able to mimic adequately some of these functions, and their use may impair tissue function in the long term... - Transcriptional profiles of valvular and vascular endothelial cells reveal phenotypic differences: influence of shear stressJonathan T Butcher
Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA
Arterioscler Thromb Vasc Biol 26:69-77. 2006.... - Tissue engineering of a collagen-based vascular media: Demonstration of functionalityStacey C Schutte
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA
Organogenesis 6:204-11. 2010..This highlights the need for functional testing of engineered tissue and the importance of cell-matrix interactions in vasoactivity... - Genetic modification of smooth muscle cells to control phenotype and function in vascular tissue engineeringJan P Stegemann
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332-0363, USA
Tissue Eng 10:189-99. 2004.... - Purified and proliferating endothelial cells derived and expanded in vitro from embryonic stem cellsKara E McCloskey
Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
Endothelium 10:329-36. 2003..Our findings indicate that it is possible to obtain proliferating populations of homogeneous endothelial cells from mouse ES cells without genetically manipulating the ES cells or coculturing with feeder cells... - Endothelial connexin 37, connexin 40, and connexin 43 respond uniquely to substrate and shear stressTiffany L Johnson
Georgia Tech Emory Center for the Engineering of Living Tissues, Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA
Endothelium 14:215-26. 2007..Individual isotypes show differential responses and RNAi knockdown provides new insight into connexin function and potential roles in the vasculature... - Vessel wall-embedded dendritic cells induce T-cell autoreactivity and initiate vascular inflammationJi W Han
Kathleen B and Mason I Lowance Center for Human Immunology, Emory University School of Medicine, Atlanta, GA 30322, USA
Circ Res 102:546-53. 2008..DCs are superior in sensing pathogen-derived motifs and are highly efficient in breaking T-cell tolerance, guiding T cells toward proinflammatory and tissue-invasive behavior... - Mechanical strain-stimulated remodeling of tissue-engineered blood vessel constructsDror Seliktar
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA
Tissue Eng 9:657-66. 2003..Taken together, these results illustrate the importance of mechanical stimulus as a major regulatory component of tissue-engineered blood vessel remodeling... - Unique morphology and focal adhesion development of valvular endothelial cells in static and fluid flow environmentsJonathan T Butcher
Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
Arterioscler Thromb Vasc Biol 24:1429-34. 2004.... - A biological hybrid model for collagen-based tissue engineered vascular constructsJoseph D Berglund
School of Chemical Engineering, Georgia Institute of Technology, 30332, Atlanta, GA, USA
Biomaterials 24:1241-54. 2003..Construct compaction, cell viability, and histological examination demonstrated that the function of most cells remained unimpaired with the incorporation of the biological support sleeve... - Differences in valvular and vascular cell responses to strain in osteogenic mediaZannatul Ferdous
Institute of Bioengineering and Biosciences, Georgia Institute of Technology, 315 Ferst Drive, Mail code 0363, Atlanta, GA 30332, USA
Biomaterials 32:2885-93. 2011..Further investigation is necessary for improved understanding and to detect biomarkers for early detection or prevention of these diseases... - Cyclic strain improves strength and function of a collagen-based tissue-engineered vascular mediaStacey C Schutte
Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
Tissue Eng Part A 16:3149-57. 2010..Mechanical stimulation promoted a more contractile phenotype and led to a greater contractile response to the vasoconstrictor endothelin-1... - Poly(glycerol sebacate) supports the proliferation and phenotypic protein expression of primary baboon vascular cellsJin Gao
Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
J Biomed Mater Res A 83:1070-5. 2007..These results suggest that PGS is an appropriate scaffold material for blood vessel tissue engineering... - Fluid-induced shear stress stimulates chondrocyte proliferation partially mediated via TGF-beta1Prasanna Malaviya
Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA
Tissue Eng 8:581-90. 2002..These results show that fluid shear stress upregulates chondrocyte proliferation and that this effect is partially mediated by TGF-beta1... - The role of matrix metalloproteinase-2 in the remodeling of cell-seeded vascular constructs subjected to cyclic strainD Seliktar
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, USA
Ann Biomed Eng 29:923-34. 2001..Our observations suggest that a better understanding of the complex relation between mechanical stimulation and construct remodeling is key for the proper design of tissue-engineered blood vessel substitutes... - Dynamic mechanical conditioning of collagen-gel blood vessel constructs induces remodeling in vitroD Seliktar
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta 30332 0363, USA
Ann Biomed Eng 28:351-62. 2000..This concept, in conjunction with a proper biochemical environment, could present a better model for engineering vascular constructs... - Maintenance of a functional endothelial cell monolayer on a fibroblast/polymer substrate under physiologically relevant shear stress conditionsLinda G Braddon
Georgia Institute of Technology, Atlanta, Georgia, USA
Tissue Eng 8:695-708. 2002..These results demonstrate that a fibroblast/polymer scaffold can serve as a suitable substrate for ECs and that such a monolayer demonstrates the normal EC responsiveness to flow... - Tissue engineering: the hope, the hype, and the futureRobert M Nerem
Georgia Tech/Emory Center for the Engineering of Living Tissues, Georgia Institute of Technology, Atlanta, Georgia, USA
Tissue Eng 12:1143-50. 2006 - The response of endothelial cells to fluid shear stress using a co-culture model of the arterial wallB Imberti
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA
Endothelium 9:11-23. 2002..These results suggest that EC response to fluid shear stress in a collagen co-culture model is influenced by the underlying substrate, and one that in this study is modified by strain preconditioning... - Bioengineered tissues: the science, the technology, and the industryT Ahsan
Georgia Tech Emory Center for the Engineering of Living Tissues, Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 0363, USA
Orthod Craniofac Res 8:134-40. 2005..The goal is the repair, replacement, and/or the regeneration of tissues and organs. The objective of this paper is to identify and discuss the major issues that have become apparent... - Tissue engineering: confronting the transplantation crisisRobert M Nerem
Georgia Tech/Emory Center For the Engineering of Living Tissue, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332-0363, USA
Adv Exp Med Biol 534:1-9. 2003 - Mechanical compression alters gene expression and extracellular matrix synthesis by chondrocytes cultured in collagen I gelsChristopher J Hunter
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta 30332-0535, USA
Biomaterials 23:1249-59. 2002..This suggests that mechanical stimuli may be useful in modulating chondrocyte metabolism in tissue engineering systems using fibrillar protein scaffolds... - Uniaxial strain upregulates matrix-degrading enzymes produced by human vascular smooth muscle cellsKazuhiko Asanuma
Division of Cardiology, Department of Medicine, Emory University School of Medicine, 1639 Pierce Drive, Atlanta, GA 30332, USA
Am J Physiol Heart Circ Physiol 284:H1778-84. 2003..We suggest that local increases in stationary mechanical strain resulting from stenting, hypertension, or atherosclerosis may lead to enhanced matrix degradation by SMC... - Modeling of cryopreservation of engineered tissues with one-dimensional geometryZ F Cui
Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, USA
Biotechnol Prog 18:354-61. 2002..The outcome of such a model study can be used to evaluate the construct design on its survivability during cryopreservation and to select a cryopreservation protocol to achieve maximum cell survival... - Tissue engineering: confronting the transplantation crisisR M Nerem
Georgia Tech Emory Center for the Engineering of Living Tissues, Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta 30332 0363, USA
Proc Inst Mech Eng H 214:95-9. 2000..With the development of these core technologies, tissue engineering will evolve from an art form to a technology based on science and engineering... - Potential of baboon endothelial progenitor cells for tissue engineered vascular graftsMonica T Hinds
Department of Biomedical Engineering, Oregon Health and Science University, Portland, Oregon 97239, USA
J Biomed Mater Res A 86:804-12. 2008..Thus while the lack of eNOS expression is worthy of investigation, EPCs are an attractive cell source for tissue engineered vascular grafts and the baboon model has great potential for continuing evaluations of these cells... - Editorial: advancing tissue engineering and regenerative medicineKiki B Hellman
Tissue Eng 13:2823-4. 2007 - Valvular endothelial cells and the mechanoregulation of valvular pathologyJonathan T Butcher
Department of Biomedical Engineering, 270 Olin Hall, Cornell University, Ithaca, NY 14850, USA
Philos Trans R Soc Lond B Biol Sci 362:1445-57. 2007..Further understanding of these mechanisms may help lead to earlier diagnostic tools and therapeutic strategies...