Research Topics
| Alfred H Merrill JrSummaryAffiliation: Georgia Institute of Technology Country: USA Publications
Research Grants
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Detail Information
Publications
De novo sphingolipid biosynthesis: a necessary, but dangerous, pathwayAlfred H Merrill
School of Biology, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia 30332-0230, USA
J Biol Chem 277:25843-6. 2002- Sphingoid bases and de novo ceramide synthesis: enzymes involved, pharmacology and mechanisms of actionDavid S Menaldino
Department of Chemistry, Emory University, Atlanta, GA 30322-0230, USA
Pharmacol Res 47:373-81. 2003..Through studying such compounds, there is now a greater understanding of the metabolism and mechanism(s) of action of naturally occurring sphingoid bases as well as of these analogs... 
Characterization of ceramide synthase 2: tissue distribution, substrate specificity, and inhibition by sphingosine 1-phosphateElad L Laviad
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 283:5677-84. 2008....
An introduction to sphingolipid metabolism and analysis by new technologiesYanfeng Chen
School of Chemistry and Biochemistry, The Wallace H Coulter Department of Biomedical Engineering and the Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
Neuromolecular Med 12:306-19. 2010..This review summarizes the basic metabolism of SP and state-of-art mass spectrometric techniques that are producing insights into SP structure, metabolism, functions, and some of the dysfunctions of relevance to neuromedicine...- Elevation of sulfatides in ovarian cancer: an integrated transcriptomic and lipidomic analysis including tissue-imaging mass spectrometryYing Liu
School of Biology and the Petit Institute for Bioscience and Bioengineering, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, Georgia 30332 0363, USA
Mol Cancer 9:186. 2010.... - Integration of lipidomics and transcriptomics data towards a systems biology model of sphingolipid metabolismShakti Gupta
Department of Bioengineering, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA
BMC Syst Biol 5:26. 2011.... - (Glyco)sphingolipidology: an amazing challenge and opportunity for systems biologyAlfred H Merrill
School of Biology, Chemistry and Biochemistry, The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
Trends Biochem Sci 32:457-68. 2007..Dealing with such large data sets poses special challenges for systems biology, but the intrinsic and elegant interrelationships among these compounds might provide a key to dealing with the complexity of the sphingolipidome... - Sphingolipidomics: high-throughput, structure-specific, and quantitative analysis of sphingolipids by liquid chromatography tandem mass spectrometryAlfred H Merrill
School of Biology, Georgia Institute of Technology, Atlanta, GA 30332 0230, USA
Methods 36:207-24. 2005..Also discussed are some of the issues remaining to be resolved in the analysis of the full sphingolipidome... - Sphingolipidomics: a valuable tool for understanding the roles of sphingolipids in biology and diseaseAlfred H Merrill
School of Biology, Georgia Institute of Technology, Atlanta, GA 30332, USA
J Lipid Res 50:S97-102. 2009.... - Computational modeling of a metabolic pathway in ceramide de novo synthesisShobhika Dhingra
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Conf Proc IEEE Eng Med Biol Soc 2007:1405-8. 2007..Our future work includes more experimental validation of the model, dynamic rate constants assessment, and expansion of the model to include additional enzymes in the ceramide de novo synthesis pathway... - Quantitative analysis of sphingolipids for lipidomics using triple quadrupole and quadrupole linear ion trap mass spectrometersRebecca L Shaner
Schools of Chemistry and Biochemistry, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332 0230, USA
J Lipid Res 50:1692-707. 2009..The application of these methods is illustrated with RAW264.7 cells, a mouse macrophage cell line. These methods should be useful for a wide range of focused (sphingo)lipidomic investigations... - An interactive visualization tool and data model for experimental design in systems biologyShray Kapoor
College of Computing, Georgia Institute of Technology, Atlanta, GA 30332, USA
Conf Proc IEEE Eng Med Biol Soc 2008:2423-6. 2008..We aim to build a comprehensive and interactive communication platform to improve experimental design for scientists globally in high-throughput lipid systems biology research... - Adaptive control model reveals systematic feedback and key molecules in metabolic pathway regulationChang F Quo
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
J Comput Biol 18:169-82. 2011..This new modeling approach and the implementation routines designed for this case study may be extended to other systems. Supplementary Material is available at www.liebertonline.com/cmb ... - Ceramides and other bioactive sphingolipid backbones in health and disease: lipidomic analysis, metabolism and roles in membrane structure, dynamics, signaling and autophagyWenjing Zheng
School of Biology, Chemistry and Biochemistry, Petit Institute of Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332-0230, USA
Biochim Biophys Acta 1758:1864-84. 2006.... - Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodiesHyejung Park
School of Biology and Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
J Lipid Res 51:480-9. 2010.... - Biodiversity of sphingoid bases ("sphingosines") and related amino alcoholsSarah T Pruett
Department of Chemistry, Emory University, Atlanta, GA 30322, USA
J Lipid Res 49:1621-39. 2008..This thematic review gives an overview of the biodiversity of the backbones of sphingolipids and the broader field of naturally occurring and synthetic sphingoid base-like compounds... - Fumonisins and fumonisin analogs as inhibitors of ceramide synthase and inducers of apoptosisKena Desai
School of Biology, Georgia Institute of Technology, Atlanta, GA 30332-0230, USA
Biochim Biophys Acta 1585:188-92. 2002..These findings underscore the complexity of sphingolipid metabolism and the difficulty of identifying the pertinent mediators unless a full profile of the potentially bioactive species is evaluated... - Structure-specific, quantitative methods for analysis of sphingolipids by liquid chromatography-tandem mass spectrometry: "inside-out" sphingolipidomicsM Cameron Sullards
School of Biology, and the Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA
Methods Enzymol 432:83-115. 2007.... - Cyclic AMP-stimulated interaction between steroidogenic factor 1 and diacylglycerol kinase theta facilitates induction of CYP17Donghui Li
School of Biology, Georgia Institute of Technology, Atlanta, GA 30332 0230, USA
Mol Cell Biol 27:6669-85. 2007..We conclude that ACTH/cAMP stimulates PA production in the nucleus of H295R cells and that this increase in PA concentrations facilitates CYP17 induction... - Imaging MALDI mass spectrometry using an oscillating capillary nebulizer matrix coating system and its application to analysis of lipids in brain from a mouse model of Tay-Sachs/Sandhoff diseaseYanfeng Chen
School of Chemistry and Biochemistry, The Parker H Petit Institute for Bioengineering and Bioscience, 315 Ferst Drive, Georgia Institute of Technology, Atlanta, Georgia 30332 0363, USA
Anal Chem 80:2780-8. 2008..These results illustrate the usefulness of tissue-imaging MALDI-MS with matrix deposition by OCN for histologic comparison of lipids in tissues such as brains from this mouse model of Tay-Sachs and Sandhoff disease... - Synthesis of 1-deoxysphingosine derivatives with conformationally restricted pyrrolidinediol head groupsAnn M Dougherty
Department of Chemistry, Emory University, Atlanta, Georgia 30322, USA
Org Lett 8:649-52. 2006..The synthetic chemistry for pyrrolidine ring closure at C3-C4 features ring-closing metathesis followed by hydroboration-oxidation... - ACTH regulates steroidogenic gene expression and cortisol biosynthesis in the human adrenal cortex via sphingolipid metabolismTuba Ozbay
School of Biology, Parker H Institute for Bioengineering and Biosciences, Georgia Institute of Technology, Atlanta, Georgia, USA
Endocr Res 30:787-94. 2004..Finally, these findings suggest that sphingolipids may serve as signaling mediators in ACTH-stimulated cortisol biosynthesis... - Steroidogenic factor-1 is a sphingolipid binding proteinAarti N Urs
School of Biology and the Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 310 Ferst Drive, Atlanta, GA 30332 0230, United States
Mol Cell Endocrinol 265:174-8. 2007..Finally, these findings highlight a central role for bioactive lipids as mediators of trophic hormone-stimulated steroid hormone biosynthesis... - Enigmol: a novel sphingolipid analogue with anticancer activity against cancer cell lines and in vivo models for intestinal and prostate cancerHolly Symolon
School of Biology, 310 Ferst Drive, Georgia Institute of Technology, Atlanta, GA 30332, USA
Mol Cancer Ther 10:648-57. 2011..Thus, Enigmol represents a novel category of sphingoid base analogue that is orally bioavailable and has the potential to be effective against multiple types of cancer... - Dietary soy sphingolipids suppress tumorigenesis and gene expression in 1,2-dimethylhydrazine-treated CF1 mice and ApcMin/+ miceHolly Symolon
Program in Nutrition and Health Science, Emory University, Atlanta, GA 30322, USA
J Nutr 134:1157-61. 2004..Because soy contains relatively high amounts of GlcCer, sphingolipids may partially account for the anticancer benefits attributed to soy-based foods... - Serine palmitoyltransferase subunit 1 is present in the endoplasmic reticulum, nucleus and focal adhesions, and functions in cell morphologyJia Wei
Petit Institute for Bioengineering and Bioscience, Atlanta, GA 30332, USA
Biochim Biophys Acta 1791:746-56. 2009..Thus, in addition to its "traditional" localization in the ER for de novo sphingolipid biosynthesis, SPT1 is present in other cellular compartments, including focal adhesions where it is associated with cell morphology... - Quantitation of fatty acyl-coenzyme As in mammalian cells by liquid chromatography-electrospray ionization tandem mass spectrometryChristopher A Haynes
School of Biology, Georgia Institute of Technology, Atlanta, GA 30332 0230, USA
J Lipid Res 49:1113-25. 2008..This simple and robust method should facilitate the inclusion of this family of compounds in "lipidomics" and "metabolomics" studies... - Kdo2-lipid A, a TLR4-specific agonist, induces de novo sphingolipid biosynthesis in RAW264.7 macrophages, which is essential for induction of autophagyKacee Sims
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, USA
J Biol Chem 285:38568-79. 2010.... - Safingol toxicology after oral administration to TRAMP mice: demonstration of safingol uptake and metabolism by N-acylation and N-methylationPablo R Morales
Division of Animal Resources, Emory University School of Medicine, Atlanta, Georgia 30322, USA
Drug Chem Toxicol 30:197-216. 2007..Safingol and its N-methyl metabolites were cytotoxic to both a human prostate cell line (DU145) and mouse BALB 3T3 cells; therefore, the host and potential antitumor toxicity may be due to multiple molecular species of safingol... - Imaging MALDI mass spectrometry of sphingolipids using an oscillating capillary nebulizer matrix application systemYanfeng Chen
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA
Methods Mol Biol 656:131-46. 2010..In addition, the observation of numerous lipid subclasses with distinct localizations in the brain slices demonstrates that imaging MALDI-MS could be effectively used for "lipidomic" studies... - Sphingosine-phosphate lyase enhances stress-induced ceramide generation and apoptosisUlrike Reiss
Children s Hospital Oakland Research Institute, Oakland, California 94609 1673, USA
J Biol Chem 279:1281-90. 2004.... - Two mammalian longevity assurance gene (LAG1) family members, trh1 and trh4, regulate dihydroceramide synthesis using different fatty acyl-CoA donorsChristian Riebeling
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 278:43452-9. 2003..and Futerman, A. H. (2002) FEBS Lett. 528, 3-4) that Lag1p family members regulate (dihydro)ceramide synthases responsible for production of sphingolipids containing different fatty acids... - Diabetes alters sphingolipid metabolism in the retina: a potential mechanism of cell death in diabetic retinopathyTodd E Fox
Department of Pharmacology H078, Penn State College of Medicine, Hershey, PA 17033, USA
Diabetes 55:3573-80. 2006..Thus, dysfunctional glycosphingolipid metabolism may contribute to metabolic stress in diabetes, and therapeutic strategies to restore normal sphingolipid metabolism may be a viable approach for treatment of diabetic retinopathy... - N-(4-Hydroxyphenyl)retinamide increases dihydroceramide and synergizes with dimethylsphingosine to enhance cancer cell killingHongtao Wang
Childrens Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, California, USA
Mol Cancer Ther 7:2967-76. 2008..These studies suggest that enhanced clinical efficacy of 4-HPR may be realized through regimens containing agents that modulate sphingoid base metabolism... - Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cellsKrishnan Venkataraman
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel
J Biol Chem 277:35642-9. 2002.... - Activity of partially inhibited serine palmitoyltransferase is sufficient for normal sphingolipid metabolism and viability of HSN1 patient cellsVadim N Dedov
Neurobiology Laboratory, ANZAC Research Institute, Concord Repatriation General Hospital, Hospital Road, Concord, NSW 2139, Australia
Biochim Biophys Acta 1688:168-75. 2004.... - Sphingolipid metabolism and signaling minireview seriesWilliam L Smith
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan 48824, USA
J Biol Chem 277:25841-2. 2002 - Fumonisins disrupt sphingolipid metabolism, folate transport, and neural tube development in embryo culture and in vivo: a potential risk factor for human neural tube defects among populations consuming fumonisin-contaminated maizeWalter F O Marasas
Medical Research Council, PROMEC Unit, Tygerberg 7505, South Africa
J Nutr 134:711-6. 2004..Hence, we propose that fumonisins are potential risk factors for NTD, craniofacial anomalies, and other birth defects arising from neural crest cells because of their apparent interference with folate utilization... - Cooperative prosurvival activity by ERK and Akt in human alveolar macrophages is dependent on high levels of acid ceramidase activityMartha M Monick
Division of Pulmonary, Critical Care, and Occupational Medicine, Roy J and Lucille A Carver College of Medicine, Eckstein Medical Research Building Room 100, University of Iowa, Iowa City, IA 52242, USA
J Immunol 173:123-35. 2004..These studies suggest an important role for sphingolipids in prolonging survival of human alveolar macrophages via distinct survival pathways... - A comprehensive classification system for lipidsEoin Fahy
San Diego Supercomputer Center, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0505, USA
J Lipid Res 46:839-61. 2005..This structured vocabulary will facilitate the systematization of lipid biology and enable the cataloging of lipids and their properties in a way that is compatible with other macromolecular databases... - Ceramide transfer protein function is essential for normal oxidative stress response and lifespanRaghavendra Pralhada Rao
Laboratory of Cell and Developmental Signaling, National Cancer Institute Frederick, Frederick, MD 21702, USA
Proc Natl Acad Sci U S A 104:11364-9. 2007.... - LMSD: LIPID MAPS structure databaseManish Sud
LIPID MAPS Bioinformatics Core, San Diego Supercomputer Center San Diego, La Jolla, CA 92093, USA
Nucleic Acids Res 35:D527-32. 2007..Search results, in addition to structure and annotations, also include relevant links to external databases. The LMSD is publicly available at www.lipidmaps.org/data/structure/... - Hydrolyzed fumonisins HFB1 and HFB2 are acylated in vitro and in vivo by ceramide synthase to form cytotoxic N-acyl-metabolitesMichaela Seiferlein
Institute for Food Chemistry, University of Munster, Munster, Germany
Mol Nutr Food Res 51:1120-30. 2007..The contribution of acylated HFB1 and HFB2 metabolites to fumonisin toxicity in vivo warrants further investigation... - Aging up-regulates expression of inflammatory mediators in mouse adipose tissueDayong Wu
Nutritional Immunology Laboratory, Jean Mayer Unites States Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111, USA
J Immunol 179:4829-39. 2007.... - The cell surface glycosphingolipids SSEA-3 and SSEA-4 are not essential for human ESC pluripotencySandii N Brimble
Novocell, Athens, Georgia, USA
Stem Cells 25:54-62. 2007.... - Effects of sphingosine-1-phosphate and ceramide-1-phosphate on rat intestinal smooth muscle cells: implications for postoperative ileusMihaela Dragusin
, University Bonn, Germany
FASEB J 20:1930-2. 2006..Thus, the sustained elevated concentration of the two bioactive sphingolipids in this tissue could at least in part explain postoperative intestinal dysmotility... - Sphingosine-1-phosphate phosphohydrolase regulates endoplasmic reticulum-to-golgi trafficking of ceramidePaola Giussani
Department of Biochemistry, Virginia Commonwealth University School of Medicine, 1101 E Marshall Street, Room 2 011 Sanger Hall, Richmond, VA 23298 0614, USA
Mol Cell Biol 26:5055-69. 2006..Our results suggest that SPP-1 regulates ceramide levels in the ER and thus influences the anterograde membrane transport of both ceramide and proteins from the ER to the Golgi apparatus... - SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolismMichael Maceyka
Department of Biochemistry, Virginia Commonwealth University School of Medicine, Richmond, Virginia 23298, USA
J Biol Chem 280:37118-29. 2005..Our results demonstrate that SphK1 and SphK2 have opposing roles in the regulation of ceramide biosynthesis and suggest that the location of sphingosine 1-phosphate production dictates its functions... - Human cytomegalovirus regulates bioactive sphingolipidsNicholas J Machesky
Department of Pathology, The Ohio State University, Columbus, Ohio 43210, USA
J Biol Chem 283:26148-60. 2008..Together, these findings demonstrate that host cell sphingolipids are dynamically regulated upon infection with a herpes virus in a manner that impacts virus replication... - Ceramide kinase uses ceramide provided by ceramide transport protein: localization to organelles of eicosanoid synthesisNadia F Lamour
Department of Biochemistry, Medical College of Virginia, Virginia Commonwealth University, Richmond, VA 23298, USA
J Lipid Res 48:1293-304. 2007..In conclusion, these results demonstrate that CERK localizes to areas of eicosanoid synthesis and uses a ceramide "pool" transported in an active manner via CERT...
Research Grants
- STRUCTURE/FUNCTION STUDIES OF LONG-CHAIN BASESALFRED MERRILL; Fiscal Year: 2003....
- MODULATION BY GROWTH FACTORS AND SIGNAL TRANSDUCTIONALFRED MERRILL; Fiscal Year: 2003..abstract_text> ..
- Role of Small GTPases in Muscarinic Receptor TraffickingALFRED MERRILL; Fiscal Year: 2005..abstract_text> ..
- 2006 Glycolipid & Sphingolipid Gordon ConferenceALFRED MERRILL; Fiscal Year: 2006....
- Characterization of mammalian ceramide synthasesALFRED MERRILL; Fiscal Year: 2007....
- 2006 Glycolipid & Sphingolipid Gordon ConferenceALFRED MERRILL; Fiscal Year: 2007..abstract_text> ..
- Characterization of mammalian ceramide synthasesALFRED MERRILL; Fiscal Year: 2009....
- Gordon Conference on Glycolipid & Sphingolipid BiologyALFRED MERRILL; Fiscal Year: 2002....
- STRUCTURE/FUNCTION STUDIES OF LONG-CHAIN BASESALFRED MERRILL; Fiscal Year: 1993..These investigations will provide fundamental information about the biochemistry and cell biology of sphingosine, and could provide valuable tools and insight into long-chain bases as modulators of cell behavior...
- STRUCTURE/FUNCTION STUDIES OF LONG-CHAIN BASESALFRED MERRILL; Fiscal Year: 1999..These investigations will provide fundamental information about the biochemistry and cell biology of sphingosine, and could provide valuable tools and insight into long-chain bases as modulators of cell behavior. ..
- Characterization of mammalian ceramide synthasesALFRED HARRISON MERRILL; Fiscal Year: 2010....