Hanjoong Jo

Summary

Affiliation: Georgia Institute of Technology
Country: USA

Publications

  1. pmc Differential proinflammatory and prooxidant effects of bone morphogenetic protein-4 in coronary and pulmonary arterial endothelial cells
    Anna Csiszar
    Dept of Physiology, New York Medical College, Valhalla, New York 10595, USA
    Am J Physiol Heart Circ Physiol 295:H569-77. 2008
  2. pmc Partial carotid ligation is a model of acutely induced disturbed flow, leading to rapid endothelial dysfunction and atherosclerosis
    Douglas Nam
    Division of Cardiology, Department of Medicine, Emory University, Atlanta, GA, USA
    Am J Physiol Heart Circ Physiol 297:H1535-43. 2009
  3. ncbi request reprint Flow-dependent regulation of endothelial nitric oxide synthase: role of protein kinases
    Yong Chool Boo
    Wallace H Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, GA 30322, USA
    Am J Physiol Cell Physiol 285:C499-508. 2003
  4. ncbi request reprint Role of NADPH oxidases in disturbed flow- and BMP4- induced inflammation and atherosclerosis
    Hanjoong Jo
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, 30322, USA
    Antioxid Redox Signal 8:1609-19. 2006
  5. ncbi request reprint Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress induces monocyte adhesion by stimulating reactive oxygen species production from a nox1-based NADPH oxidase
    George P Sorescu
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    Circ Res 95:773-9. 2004
  6. ncbi request reprint Oscillatory shear stress stimulates endothelial production of O2- from p47phox-dependent NAD(P)H oxidases, leading to monocyte adhesion
    Jinah Hwang
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    J Biol Chem 278:47291-8. 2003
  7. ncbi request reprint Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and at
    Kyunghwa Chang
    Wallace H Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, GA 30322, USA
    Circulation 116:1258-66. 2007
  8. pmc Dynamic immune cell accumulation during flow-induced atherogenesis in mouse carotid artery: an expanded flow cytometry method
    Noah Alberts-Grill
    School of Medicine, Emory University, Woodruff Memorial Bldg, Rm 2005, Atlanta, GA 30322, USA
    Arterioscler Thromb Vasc Biol 32:623-32. 2012
  9. pmc Tetrahydrobiopterin deficiency and nitric oxide synthase uncoupling contribute to atherosclerosis induced by disturbed flow
    Li Li
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA
    Arterioscler Thromb Vasc Biol 31:1547-54. 2011
  10. ncbi request reprint Bone morphogenic protein-4 induces hypertension in mice: role of noggin, vascular NADPH oxidases, and impaired vasorelaxation
    Sumitra Miriyala
    Division of Cardiology, Emory University, Emory University, Atlanta, GA 30322, USA
    Circulation 113:2818-25. 2006

Research Grants

Collaborators

Detail Information

Publications69

  1. pmc Differential proinflammatory and prooxidant effects of bone morphogenetic protein-4 in coronary and pulmonary arterial endothelial cells
    Anna Csiszar
    Dept of Physiology, New York Medical College, Valhalla, New York 10595, USA
    Am J Physiol Heart Circ Physiol 295:H569-77. 2008
    ..The vascular bed-specific endothelial effects of BMP-4 are likely to contribute to its differential pathophysiological role in the systemic and pulmonary circulation...
  2. pmc Partial carotid ligation is a model of acutely induced disturbed flow, leading to rapid endothelial dysfunction and atherosclerosis
    Douglas Nam
    Division of Cardiology, Department of Medicine, Emory University, Atlanta, GA, USA
    Am J Physiol Heart Circ Physiol 297:H1535-43. 2009
    ..It also allows for easy and rapid intimal RNA isolation. This novel model and method could be used for genome-wide studies to determine molecular mechanisms underlying flow-dependent regulation of vascular biology and diseases...
  3. ncbi request reprint Flow-dependent regulation of endothelial nitric oxide synthase: role of protein kinases
    Yong Chool Boo
    Wallace H Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, GA 30322, USA
    Am J Physiol Cell Physiol 285:C499-508. 2003
    ..The functional significance of each phosphorylation site is still unclear. We have attempted to summarize the current status of understanding in shear-dependent eNOS regulation...
  4. ncbi request reprint Role of NADPH oxidases in disturbed flow- and BMP4- induced inflammation and atherosclerosis
    Hanjoong Jo
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, 30322, USA
    Antioxid Redox Signal 8:1609-19. 2006
    ..This emerging field of shear stress, BMP4, NADPH oxidases, inflammation, and atherosclerosis is reviewed...
  5. ncbi request reprint Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress induces monocyte adhesion by stimulating reactive oxygen species production from a nox1-based NADPH oxidase
    George P Sorescu
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    Circ Res 95:773-9. 2004
    ..Together, these results suggest that BMP4 produced in ECs by OS stimulates ROS release from the nox1-dependent NADPH oxidase leading to inflammation, a critical early atherogenic step...
  6. ncbi request reprint Oscillatory shear stress stimulates endothelial production of O2- from p47phox-dependent NAD(P)H oxidases, leading to monocyte adhesion
    Jinah Hwang
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    J Biol Chem 278:47291-8. 2003
    ....
  7. ncbi request reprint Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and at
    Kyunghwa Chang
    Wallace H Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, Atlanta, GA 30322, USA
    Circulation 116:1258-66. 2007
    ..BMP activity is regulated by the level of BMP antagonists. Until now it was not known whether shear also regulates the expression of BMP antagonists and whether they play a role in EC pathophysiology...
  8. pmc Dynamic immune cell accumulation during flow-induced atherogenesis in mouse carotid artery: an expanded flow cytometry method
    Noah Alberts-Grill
    School of Medicine, Emory University, Woodruff Memorial Bldg, Rm 2005, Atlanta, GA 30322, USA
    Arterioscler Thromb Vasc Biol 32:623-32. 2012
    ..However, the detailed changes in the composition and quantity of leukocytes in the arterial wall during atherogenesis are not fully understood in part because of the lack of suitable methods and animal models...
  9. pmc Tetrahydrobiopterin deficiency and nitric oxide synthase uncoupling contribute to atherosclerosis induced by disturbed flow
    Li Li
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA
    Arterioscler Thromb Vasc Biol 31:1547-54. 2011
    ..We therefore aimed to determine whether atherosclerosis induced by disturbed flow is due to BH(4) deficiency and NOS uncoupling and whether increasing BH(4) would prevent endothelial dysfunction, plaque inflammation, and atherosclerosis...
  10. ncbi request reprint Bone morphogenic protein-4 induces hypertension in mice: role of noggin, vascular NADPH oxidases, and impaired vasorelaxation
    Sumitra Miriyala
    Division of Cardiology, Emory University, Emory University, Atlanta, GA 30322, USA
    Circulation 113:2818-25. 2006
    ....
  11. pmc Laminar shear inhibits tubule formation and migration of endothelial cells by an angiopoietin-2 dependent mechanism
    Sarah L Tressel
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
    Arterioscler Thromb Vasc Biol 27:2150-6. 2007
    ..We hypothesized that LS inhibits EC migration and tubule formation, 2 functions important in angiogenesis, by inhibiting the secretion of proangiogenic factors...
  12. pmc Angiopoietin-2 stimulates blood flow recovery after femoral artery occlusion by inducing inflammation and arteriogenesis
    Sarah L Tressel
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University, Atlanta, GA 30322, USA
    Arterioscler Thromb Vasc Biol 28:1989-95. 2008
    ..We hypothesized that Ang2 plays an important role in blood flow recovery after arterial occlusion in vivo by regulating angiogenesis and arteriogenesis...
  13. pmc Elevated cyclic stretch induces aortic valve calcification in a bone morphogenic protein-dependent manner
    Kartik Balachandran
    The Wallace H Coulter School of Biomedical Engineering, Georgia Institute of Technology, 313 Ferst Dr, Suite 1121, Atlanta, GA 30332 0535, USA
    Am J Pathol 177:49-57. 2010
    ..These results underline the potential role of BMPs in valve calcification due to altered stretch...
  14. pmc Expression of CYP1A1 and CYP1B1 in human endothelial cells: regulation by fluid shear stress
    Daniel E Conway
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology Emory University School of Medicine, 313 Ferst Drive, Suite 2116, Atlanta, GA 30332 0535, USA
    Cardiovasc Res 81:669-77. 2009
    ....
  15. pmc HuR regulates the expression of stress-sensitive genes and mediates inflammatory response in human umbilical vein endothelial cells
    Won Jong Rhee
    Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Proc Natl Acad Sci U S A 107:6858-63. 2010
    ..Taken together, these results suggest that HuR plays a critical role in inducing inflammatory response of endothelial cells under mechanical and biochemical stresses...
  16. pmc GTP cyclohydrolase I phosphorylation and interaction with GTP cyclohydrolase feedback regulatory protein provide novel regulation of endothelial tetrahydrobiopterin and nitric oxide
    Li Li
    Division of Cardiology, Emory University School of Medicine, 1639 Pierce Dr WMBR 319, Atlanta, GA 30322, USA
    Circ Res 106:328-36. 2010
    ....
  17. pmc Early determinants of H2O2-induced endothelial dysfunction
    Beth M Boulden
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    Free Radic Biol Med 41:810-7. 2006
    ..Dysfunction required O(2)(*-), ONOO(-), or a functional NADPH oxidase. Repeated activation of the NADPH oxidase by ROS may act as a feed forward system to promote endothelial dysfunction...
  18. pmc Discovery of shear- and side-specific mRNAs and miRNAs in human aortic valvular endothelial cells
    Casey J Holliday
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA
    Am J Physiol Heart Circ Physiol 301:H856-67. 2011
    ..Using miRWalk and several filtering steps, we identified shear-sensitive mRNAs potentially targeted by shear-sensitive miRNAs. These genes and signaling pathways could act as therapeutic targets of AV disease...
  19. pmc Discovery of novel mechanosensitive genes in vivo using mouse carotid artery endothelium exposed to disturbed flow
    Chih Wen Ni
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA
    Blood 116:e66-73. 2010
    ..Determining the functional importance of these novel mechanosensitive genes may provide important insights into understanding vascular biology and atherosclerosis...
  20. doi request reprint Elevated cyclic stretch alters matrix remodeling in aortic valve cusps: implications for degenerative aortic valve disease
    Kartik Balachandran
    The Wallace H Coulter School of Biomedical Engineering, Georgia Inst of Technology, 313 Ferst Dr, Ste 1121, Atlanta, GA 30332 0535, USA
    Am J Physiol Heart Circ Physiol 296:H756-64. 2009
    ..This may trigger a cascade of events leading to an accelerated valve degeneration and disease progression...
  21. pmc Anti-inflammatory and antiatherogenic role of BMP receptor II in endothelial cells
    Chan Woo Kim
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    Arterioscler Thromb Vasc Biol 33:1350-9. 2013
    ..BMP actions are mediated by 2 different types of BMP receptors (BMPRI and BMPRII). Here, we show a surprising finding that loss of BMPRII expression causes endothelial inflammation and atherosclerosis...
  22. ncbi request reprint Laminar shear stress up-regulates peroxiredoxins (PRX) in endothelial cells: PRX 1 as a mechanosensitive antioxidant
    Amy L Mowbray
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    J Biol Chem 283:1622-7. 2008
    ..However, PRX 5 depletion did not. Together, these results suggest that PRX 1 is a novel mechanosensitive antioxidant, playing an important role in shear-dependent regulation of endothelial biology and atherosclerosis...
  23. doi request reprint Shear stress and plaque development
    Saurabh S Dhawan
    Division of Cardiology, Department of Medicine, Emory University, Atlanta, GA 30322, USA
    Expert Rev Cardiovasc Ther 8:545-56. 2010
    ..Clinical and experimental data are emerging that suggest the pathobiology associated with abnormal wall shear stress results in atherosclerotic plaque development and progression...
  24. pmc Piperlongumine inhibits atherosclerotic plaque formation and vascular smooth muscle cell proliferation by suppressing PDGF receptor signaling
    Dong Ju Son
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30322, USA
    Biochem Biophys Res Commun 427:349-54. 2012
    ..In conclusion, our findings demonstrate a novel, therapeutic mechanism by which PL suppresses atherosclerosis plaque formation in vivo...
  25. pmc Preferential activation of SMAD1/5/8 on the fibrosa endothelium in calcified human aortic valves--association with low BMP antagonists and SMAD6
    Randall F Ankeny
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America
    PLoS ONE 6:e20969. 2011
    ..Here, we tested the hypothesis that side-dependent activation of bone morphogenic protein (BMP) pathway in the endothelium of the ventricularis and fibrosa is associated with human AV calcification...
  26. ncbi request reprint NAD(P)H oxidase-derived hydrogen peroxide mediates endothelial nitric oxide production in response to angiotensin II
    Hua Cai
    Division of Cardiology, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Biol Chem 277:48311-7. 2002
    ..Because angiotensin II also stimulates O(2)() production from the NAD(P)H oxidase, the H(2)O(2) stimulation of NO(.) may facilitate peroxynitrite formation in response to this octapeptide...
  27. ncbi request reprint Plasma membrane requirements for 1alpha,25(OH)2D3 dependent PKC signaling in chondrocytes and osteoblasts
    Barbara D Boyan
    Wallace H Coulter Department of Biomedical Engineering at Georgia, Tech and Emory University, Georgia Institute of Technology, Atlanta, 30332 0363, USA
    Steroids 71:286-90. 2006
    ..Disruption of lipid rafts using beta-cyclodextrin blocks the activation of PKC by 1alpha,25(OH)(2)D(3) and reduces the ability of 1alpha,25(OH)(2)D(3) to regulate [(35)S]-sulfate incorporation...
  28. ncbi request reprint Endothelial NO synthase phosphorylated at SER635 produces NO without requiring intracellular calcium increase
    Yong Chool Boo
    Wallace H Coulter Department of Biomedical Engineering at Georgia Institute of Technology and Emory University, Emory University School of Medicine, Atlanta, GA 30322, USA
    Free Radic Biol Med 35:729-41. 2003
    ..PKA-dependent phosphorylation of eNOS S635 and subsequent basal NO production in a Ca2+-independent manner may play an important role in regulating vascular biology and pathophysiology...
  29. ncbi request reprint Expression of cathepsin K is regulated by shear stress in cultured endothelial cells and is increased in endothelium in human atherosclerosis
    Manu O Platt
    Wallace H Coulter Dept of Biomedical Engineering at Georgia Tech and Emory Univ, 308D WMB, Atlanta, GA 30322, USA
    Am J Physiol Heart Circ Physiol 292:H1479-86. 2007
    ..These findings suggest that cathepsin K is a mechanosensitive, extracellular matrix protease that, in turn, may be involved in arterial wall remodeling and atherosclerosis...
  30. pmc MicroRNA-663 upregulated by oscillatory shear stress plays a role in inflammatory response of endothelial cells
    Chih Wen Ni
    Coulter Department of Biomedical Engineering, Emory University, Atlanta, GA, USA
    Am J Physiol Heart Circ Physiol 300:H1762-9. 2011
    ..These OS-sensitive miRNAs may mediate atherosclerosis induced by disturbed flow...
  31. ncbi request reprint Cyclic pressure and shear stress regulate matrix metalloproteinases and cathepsin activity in porcine aortic valves
    Manu O Platt
    The Wallace H Coulter School of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA
    J Heart Valve Dis 15:622-9. 2006
    ..It was hypothesized that exposure of the aortic valves to pressure or shear stress would regulate valvular expression of proteases such as matrix metalloproteinases (MMPs) and cathepsins...
  32. pmc Endothelial metallothionein expression and intracellular free zinc levels are regulated by shear stress
    Daniel E Conway
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, Georgia 30332 0535, USA
    Am J Physiol Cell Physiol 299:C1461-7. 2010
    ..The sensitivity of intracellular free zinc to differences in shear stress suggests that intracellular zinc levels are important in the regulation of the endothelium and in the progression of vascular disease...
  33. pmc Animal, in vitro, and ex vivo models of flow-dependent atherosclerosis: role of oxidative stress
    Amir Rezvan
    Division of Cardiology, Department of Medicine, Emory University, Atlanta, Georgia 30322, USA
    Antioxid Redox Signal 15:1433-48. 2011
    ..Here, we review these flow models in the context of the role of oxidative stress in flow-dependent inflammation, a critical proatherogenic step, and atherosclerosis...
  34. ncbi request reprint Transcriptional profiles of valvular and vascular endothelial cells reveal phenotypic differences: influence of shear stress
    Jonathan T Butcher
    Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA
    Arterioscler Thromb Vasc Biol 26:69-77. 2006
    ..The similarities between valvular and vascular lesions suggest pathological initiation mediated through endothelium, but the role of hemodynamics in valvular endothelial biology is poorly understood...
  35. ncbi request reprint Identification of mechanosensitive genes in osteoblasts by comparative microarray studies using the rotating wall vessel and the random positioning machine
    Mamta J Patel
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30322, USA
    J Cell Biochem 101:587-99. 2007
    ....
  36. doi request reprint Systems analysis of the role of bone morphogenic protein 4 in endothelial inflammation
    Weiwei Yin
    Integrative BioSystems Institute, Georgia Institute of Technology, 313 Ferst Drive, Atlanta, GA 30332, USA
    Ann Biomed Eng 38:291-307. 2010
    ..In particular, it demonstrates the crucial role of Bone Morphogenic Protein 4 and p47(phox)-dependent NADPH oxidases in endothelial inflammation...
  37. ncbi request reprint Reactive oxygen species-selective regulation of aortic inflammatory gene expression in Type 2 diabetes
    Alejandra San Martin
    Division of Cardiology, Department of Medicine, Emory University, 1639 Pierce Dr, Atlanta, GA 30322, USA
    Am J Physiol Heart Circ Physiol 292:H2073-82. 2007
    ..The ability of Tempol to reverse ROS production as well as OPN and BMP-4, but not CTGF, induction suggests that DM-induced vascular inflammation involves both ROS-sensitive and -insensitive pathways...
  38. ncbi request reprint Bovine caveolin-2 cloning and effects of shear stress on its localization in bovine aortic endothelial cells
    Nolan Boyd
    Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Atlanta, Georgia 30322, USA
    Endothelium 11:189-98. 2004
    ..Therefore, the interaction of caveolin-2 with caveolin-1 could play an important role in caveolae biogenesis and shear stimulated mechano-signal transduction...
  39. pmc Differences in valvular and vascular cell responses to strain in osteogenic media
    Zannatul Ferdous
    Institute of Bioengineering and Biosciences, Georgia Institute of Technology, 315 Ferst Drive, Mail code 0363, Atlanta, GA 30332, USA
    Biomaterials 32:2885-93. 2011
    ..Further investigation is necessary for improved understanding and to detect biomarkers for early detection or prevention of these diseases...
  40. pmc Low magnitude and high frequency mechanical loading prevents decreased bone formation responses of 2T3 preosteoblasts
    Mamta J Patel
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30322, USA
    J Cell Biochem 106:306-16. 2009
    ..The mechanosensitive genes identified here provide potential targets for pharmaceutical treatments that may be used in combination with low level mechanical loading to better treat osteoporosis or disuse-induced bone loss...
  41. doi request reprint Design of an ex vivo culture system to investigate the effects of shear stress on cardiovascular tissue
    Philippe Sucosky
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Parker H Petit Biotechnology Building, Atlanta, GA 30332 0363, USA
    J Biomech Eng 130:035001. 2008
    ..Ultimately, this knowledge will permit the production of functional tissue engineered heart valves, and a better understanding of heart valve biology and disease progression...
  42. ncbi request reprint Laminar shear stress inhibits cathepsin L activity in endothelial cells
    Manu O Platt
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Emory University, 308D WMB, Atlanta, GA 30322, USA
    Arterioscler Thromb Vasc Biol 26:1784-90. 2006
    ..It is not known, however, whether shear regulates cathepsins. We examined the hypothesis that shear stress regulates cathepsin activity in endothelial cells...
  43. doi request reprint The effects of combined cyclic stretch and pressure on the aortic valve interstitial cell phenotype
    Patrick Thayer
    W H Coulter School of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA
    Ann Biomed Eng 39:1654-67. 2011
    ..Our study therefore demonstrates how cyclic stretch and pressure may possibly act together to modulate the AVIC phenotype...
  44. ncbi request reprint Embryonic stem cell-derived endothelial cells may lack complete functional maturation in vitro
    Kara E McCloskey
    The Parker H Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA
    J Vasc Res 43:411-21. 2006
    ..These results indicate that ESC-derived EC share some key characteristics of 'mature' EC, while retaining markers of alternate phenotypes including immature endothelium...
  45. pmc An ex vivo study of the biological properties of porcine aortic valves in response to circumferential cyclic stretch
    Kartik Balachandran
    The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
    Ann Biomed Eng 34:1655-65. 2006
    ..Most interestingly, the contractile and fibrotic phenotypic expression of valve interstitial cells was enhanced. These results show that circumferential cyclic stretch is a possible mediator for AVL remodeling activity...
  46. ncbi request reprint Bone morphogenic protein 4 produced in endothelial cells by oscillatory shear stress stimulates an inflammatory response
    George P Sorescu
    Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 278:31128-35. 2003
    ..We suggest that BMP4 is a mechanosensitive, inflammatory factor playing a critical role in early steps of atherogenesis in the lesion-prone areas...
  47. pmc The role of the vascular dendritic cell network in atherosclerosis
    Noah Alberts-Grill
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA
    Am J Physiol Cell Physiol 305:C1-21. 2013
    ..We further provide suggestions for distinguishing DCs from macrophages and discuss important future directions for the field. ..
  48. doi request reprint Aortic valve: mechanical environment and mechanobiology
    Sivakkumar Arjunon
    The Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Room 2119 U A Whitaker Building, 313 Ferst Drive, Atlanta, GA 30332 0535, USA
    Ann Biomed Eng 41:1331-46. 2013
    ..Here, we review the current knowledge of the local AV mechanical environment and its effect on valve biology, focusing on in vitro and ex vivo approaches...
  49. pmc Disturbed flow: p53 SUMOylation in the turnover of endothelial cells
    Wakako Takabe
    Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30322, USA
    J Cell Biol 193:805-7. 2011
    ..This leads to p53 SUMOylation and its export to the cytosol, where it binds to the antiapoptotic protein Bcl-2 to induce apoptosis...
  50. ncbi request reprint Optimization of isolation and functional characterization of primary murine aortic endothelial cells
    Richard Magid
    Wallace Coulter Department of Biomedical Engineering, Georgia Institute of Technology Emory University School of Medicine, Atlanta, GA 30322, USA
    Endothelium 10:103-9. 2003
    ....
  51. doi request reprint Strain magnitude-dependent calcific marker expression in valvular and vascular cells
    Zannatul Ferdous
    Institute of Bioengineering and Biosciences, Atlanta, GA, USA
    Cells Tissues Organs 197:372-83. 2013
    ..Our results suggest that cellular differences exist between vascular and valvular cells in their response to altered levels of cyclic strain during calcification...
  52. ncbi request reprint Regulation of growth plate chondrocytes by 1,25-dihydroxyvitamin D3 requires caveolae and caveolin-1
    Barbara D Boyan
    Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0363, USA
    J Bone Miner Res 21:1637-47. 2006
    ..We found that caveolae are required for rapid 1alpha,25(OH)(2)D(3)-dependent PKC signaling, and caveolin-1 must be present based on studies using chondrocytes from Cav-1(-/-) mice...
  53. ncbi request reprint Mechanical inhibition of RANKL expression is regulated by H-Ras-GTPase
    Janet Rubin
    Department of Medicine, Emory University School of Medicine and Veterans Affairs Medical Center, Atlanta, Georgia 30033, USA
    J Biol Chem 281:1412-8. 2006
    ..By partitioning signals this way, cells may be able to generate different downstream responses through seemingly similar signaling cascades...
  54. pmc Target accessibility and signal specificity in live-cell detection of BMP-4 mRNA using molecular beacons
    Won Jong Rhee
    Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
    Nucleic Acids Res 36:e30. 2008
    ..Possible beacon design rules are identified and approaches for enhancing target accessibility are discussed. This has significant implications to MB design for live cell mRNA detection...
  55. ncbi request reprint Role of p47(phox) in vascular oxidative stress and hypertension caused by angiotensin II
    Ulf Landmesser
    Division of Cardiology, Emory University School of Medicine and Atlanta Veterans Administration Hospital, Atlanta, GA 30322, USA
    Hypertension 40:511-5. 2002
    ..These results suggest a pivotal role of the NAD(P)H oxidase and its subunit p47(phox) in the vascular oxidant stress and the blood pressure response to angiotensin II in vivo...
  56. ncbi request reprint Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism
    Yong Chool Boo
    Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Atlanta, GA 30322, USA
    Am J Physiol Heart Circ Physiol 283:H1819-28. 2002
    ..Phosphorylation of Ser(635) may play an important role in chronic regulation of eNOS in response to mechanical and humoral stimuli...
  57. ncbi request reprint IkappaBalpha-dependent regulation of low-shear flow-induced NF-kappa B activity: role of nitric oxide
    Sumathy Mohan
    Department of Pathology, University of Texas Health Science Center, San Antonio, Texas 78229 3900, USA
    Am J Physiol Cell Physiol 284:C1039-47. 2003
    ....
  58. ncbi request reprint Chronic shear induces caveolae formation and alters ERK and Akt responses in endothelial cells
    Nolan L Boyd
    Wallace H Coulter Dept of Biomedical Engineering at Georgia Tech and Emory University, Atlanta, GA 30322, USA
    Am J Physiol Heart Circ Physiol 285:H1113-22. 2003
    ..These results suggest that chronic shear stimulates caveolae formation by translocating caveolin-1 from the Golgi to the luminal plasma membrane and alters cell signaling responses...
  59. ncbi request reprint Role of xanthine oxidoreductase and NAD(P)H oxidase in endothelial superoxide production in response to oscillatory shear stress
    J Scott McNally
    Division of Cardiology and Molecular and Systems Pharmacology Program, Emory University, Atlanta, GA 30322, USA
    Am J Physiol Heart Circ Physiol 285:H2290-7. 2003
    ..These data suggest that the NAD(P)H oxidase maintains endothelial cell XO levels and that XO is responsible for increased reactive oxygen species production in response to oscillatory shear stress...
  60. ncbi request reprint Shear stress stimulates phosphorylation of endothelial nitric-oxide synthase at Ser1179 by Akt-independent mechanisms: role of protein kinase A
    Yong Chool Boo
    Wallace H Coulter Department of Biomedical Engineering at Georgia Tech and Emory University, Atlanta, Georgia 30322, USA
    J Biol Chem 277:3388-96. 2002
    ..A coordinated interaction between Akt and PKA may be an important mechanism by which eNOS activity is regulated in response to physiological stimuli such as shear stress...
  61. ncbi request reprint Caveolin-1 is transiently dephosphorylated by shear stress-activated protein tyrosine phosphatase mu
    Jaeyoung Shin
    Department of Molecular Biology and Institute of Nanosensor and Biotechnology, Dankook University, San 8, Hannam Dong, Yongsan Ku, Seoul 140 714, Republic of Korea
    Biochem Biophys Res Commun 339:737-41. 2006
    ..We also observed that protein tyrosine phosphatase mu was transiently activated by shear stress, suggesting its role in the dephosphorylation of caveolin-1...
  62. pmc NFKB1 promoter variation implicates shear-induced NOS3 gene expression and endothelial function in prehypertensives and stage I hypertensives
    Joon Young Park
    Department of Kinesiology, College of Health Professions, Temple University, Philadelphia, Pennsylvania 19122, USA
    Am J Physiol Heart Circ Physiol 293:H2320-7. 2007
    ..We conclude that the (-94)NFKB1 I/D promoter variation contributes to the modulation of vascular function and adaptability to exercise-induced flow shear stress, most likely due to differences in NFKB1 gene transactivity...
  63. ncbi request reprint Emerging role of IGF-1R in stretch-induced neointimal hyperplasia in venous grafts
    Hannah Song
    Arterioscler Thromb Vasc Biol 27:1679-81. 2007
  64. ncbi request reprint Downregulation of bone morphogenetic protein 4 expression in coronary arterial endothelial cells: role of shear stress and the cAMP/protein kinase A pathway
    Anna Csiszar
    Department of Physiology, New York Medical College, Valhalla, New York 10595, USA
    Arterioscler Thromb Vasc Biol 27:776-82. 2007
    ..Recent studies suggested that atheroprotective levels of shear stress control endothelial BMP-4 expression; however, the underlying mechanisms remained unknown...
  65. ncbi request reprint Activation of c-Jun N-terminal kinase and apoptosis in endothelial cells mediated by endogenous generation of hydrogen peroxide
    Anup Ramachandran
    Department of Pathology, University of Alabama at Birmingham, 35294, USA
    Biol Chem 383:693-701. 2002
    ..However, a detailed analysis reveals important differences in both the duration and profile for activation of these signaling pathways...
  66. ncbi request reprint Ascorbic acid synthesis due to L-gulono-1,4-lactone oxidase expression enhances NO production in endothelial cells
    Hyo Jung Kim
    Department of Molecular Medicine, Kyungpook National University School of Medicine, Junggu, Daegu, Republic of Korea
    Biochem Biophys Res Commun 345:1657-62. 2006
    ..The present study strongly suggests that intracellular AA plays critical roles in vascular physiology through enhancing endothelial NO production...
  67. ncbi request reprint Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase
    Philip M Bauer
    Department of Pharmacology and Vascular Cell Signaling and Therapeutics Program, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA
    J Biol Chem 278:14841-9. 2003
    ..Ser-617 may not be important for directly regulating NO release but is important as a modulator of phosphorylation at other sites and protein-protein interactions...
  68. ncbi request reprint High glucose induced NF-kappaB DNA-binding activity in HAEC is maintained under low shear stress but inhibited under high shear stress: role of nitric oxide
    Sumathy Mohan
    Department of Pathology, The University of Texas Health Science Center, San Antonio, TX 78229, USA
    Atherosclerosis 171:225-34. 2003
    ..These observations explain, in part, the mechanism by means of which hyperglycemia accelerates the focal development of atherosclerotic lesions in low shear (lesion prone) areas of the arterial tree...
  69. ncbi request reprint Caveolin-1 knockout mice have increased bone size and stiffness
    Janet Rubin
    Department of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    J Bone Miner Res 22:1408-18. 2007
    ..Cell studies showed that cav-1 deficiency leads to increased osteoblast differentiation. These results suggest that cav-1 helps to maintain osteoblast progenitors in a less differentiated state...

Research Grants12

  1. How does blood flow regulate endothelial function?
    Hanjoong Jo; Fiscal Year: 2003
    ..Aim I. Define the role of caveolin in shear- dependent activation of ERK. Aim II. Determine the role of caveolae in shear-dependent activation of ERK. ..
  2. Shear Stress, BMP and Aortic Valve Inflammation
    Hanjoong Jo; Fiscal Year: 2007
    ..Identification of BMP4 as the mechanosensitive, pro-inflammatory cytokine leading to AV inflammation and calcification may provide a potential non-surgical therapeutic avenue to treat and prevent AV diseases. ..
  3. Reactive nitrogen species in shear-dependent signaling
    Hanjoong Jo; Fiscal Year: 2005
    ..Aim 2: Determine the effect of laminar shear on RNS productions. Aim 4: Define the role of NO and RNS in laminar shear-dependent, anti-atherogenic responses of endothelial cells. ..
  4. Shear Stress, BMP and Aortic Valve Inflammation
    Hanjoong Jo; Fiscal Year: 2010
    ..Identification of BMP4 as the mechanosensitive, pro-inflammatory cytokine leading to AV inflammation and calcification may provide a potential non-surgical therapeutic avenue to treat and prevent AV diseases. ..