Research Topics
| Gang BaoSummaryAffiliation: Georgia Institute of Technology Country: USA Publications
Research Grants
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Detail Information
Publications
Engineering nanostructured probes for sensitive intracellular gene detectionGang Bao
Departament of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Mech Chem Biosyst 1:23-36. 2004..The underlying biological and biochemical aspects are illustrated...
Fluorescent probes for live-cell RNA detectionGang Bao
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
Annu Rev Biomed Eng 11:25-47. 2009..It is expected that continued advancements in live cell imaging of RNA will open new and exciting opportunities in a wide range of biological and medical applications...- Hybridization of 2'-O-methyl and 2'-deoxy molecular beacons to RNA and DNA targetsAndrew Tsourkas
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Nucleic Acids Res 31:5168-74. 2003..For the pairs tested, the 2'-deoxy-beacon/DNA-target duplex showed the fastest hybridization kinetics. These findings have significant implications for the design and application of molecular beacons... - Self-assembly of phospholipid-PEG coating on nanoparticles through dual solvent exchangeSheng Tong
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, United States
Nano Lett 11:3720-6. 2011.... - Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cellsNitin Nitin
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Nucleic Acids Res 32:e58. 2004..The peptide-linked molecular beacons approach promises to open new and exciting opportunities in sensitive gene detection and quantification in vivo... - Translation inhibition reveals interaction of 2'-deoxy and 2'-O-methyl molecular beacons with mRNA targets in living cellsNitin Nitin
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Nucleic Acids Res 37:4977-86. 2009..This work may thus provide a significant insight into probe design for detection of RNA molecules in living cells and RNA biology... - Hybridization kinetics and thermodynamics of molecular beaconsAndrew Tsourkas
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Nucleic Acids Res 31:1319-30. 2003..Molecular beacons with very short stems have a lower signal-to-background ratio than molecular beacons with longer stems. These features have significant implications for the design of molecular beacons for various applications... - Hybridization of 2'-O-methyl and 2'-deoxy molecular beacons to RNA and DNA targetsAndrew Tsourkas
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Nucleic Acids Res 30:5168-74. 2002..For the pairs tested, the 2'-deoxy-beacon/DNA-target duplex showed the fastest hybridization kinetics. These findings have significant implications for the design and application of molecular beacons... 
Quantum dot-fluorescent protein FRET probes for sensing intracellular pHAllison M Dennis
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, Georgia 30332, United States
ACS Nano 6:2917-24. 2012..These probes have the potential to enjoy a wide range of intracellular pH imaging applications that may not be feasible with fluorescent proteins or organic fluorophores alone...- Spectroscopic features of dual fluorescence/luminescence resonance energy-transfer molecular beaconsAndrew Tsourkas
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
Anal Chem 75:3697-703. 2003..These new approaches allow for the ultrasensitive detection of target molecules in a way that could be readily applied to real-time imaging of gene expression in living cells... - Nanostructured probes for RNA detection in living cellsPhilip Santangelo
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA
Ann Biomed Eng 34:39-50. 2006..The nanostructured probes promise to open new and exciting opportunities in sensitive gene detection for a wide range of biological and medical applications... - Simultaneous detection of mRNA and protein stem cell markers in live cellsWon Jong Rhee
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
BMC Biotechnol 9:30. 2009..Here we report the detection of Oct-4 mRNA and SSEA-1 protein in live carcinoma stem cells using respectively molecular beacon and dye-labeled antibody, aiming to establish a new method for stem cells detection and isolation... - Target accessibility and signal specificity in live-cell detection of BMP-4 mRNA using molecular beaconsWon Jong Rhee
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Nucleic Acids Res 36:e30. 2008..Possible beacon design rules are identified and approaches for enhancing target accessibility are discussed. This has significant implications to MB design for live cell mRNA detection... - Tat peptide is capable of importing large nanoparticles across nuclear membrane in digitonin permeabilized cellsNitin Nitin
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Ann Biomed Eng 37:2018-27. 2009..These results have significant implications for the development of new approaches for delivery of cargo into the nuclei of living cells... - Dual FRET molecular beacons for mRNA detection in living cellsPhilip J Santangelo
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Nucleic Acids Res 32:e57. 2004..2. We further revealed intriguing details of K-ras and survivin mRNA localization in living cells. The dual FRET molecular beacons approach provides a novel technique for sensitive RNA detection and quantification in living cells... - Block copolymer-based gadolinium nanoparticles as MRI contrast agents with high T1 relaxivitySijian Hou
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University Atlanta, GA 30332, USA
Nanomedicine (Lond) 7:211-8. 2012..To synthesize block copolymer-based gadolinium nanoparticles (Gd-NPs) and evaluate their potential as a new MRI contrast agent... - In vitro quantification of specific microRNA using molecular beaconsMeredith B Baker
Division of Cardiology, Emory University School of Medicine, Atlanta, GA 30322, USA
Nucleic Acids Res 40:e13. 2012..These results suggest that the molecular beacon-based approach to assess miRNA expression and distinguish mature and precursor miRNA species is quite robust, and has the promise for assessing miRNA levels in biological samples... - In vivo evaluation of (64)Cu-labeled magnetic nanoparticles as a dual-modality PET/MR imaging agentCharles Glaus
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA
Bioconjug Chem 21:715-22. 2010..Biodistribution and in vivo PET/CT imaging studies of the probes showed a circulation half-life of 143 min and high initial blood retention with moderate liver uptake, making them an attractive contrast agent for disease studies... - Surface ligand effects on metal-affinity coordination to quantum dots: implications for nanoprobe self-assemblyAllison M Dennis
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
Bioconjug Chem 21:1160-70. 2010..The results of this systematic comparison can provide a basis for rational design of self-assembled biomolecule-QD complexes for biomedical applications... - Direct visualization of mRNA colocalization with mitochondria in living cells using molecular beaconsPhilip J Santangelo
Georgia Institute of Technology and Emory University, Department of Biomedical Engineering, Atlanta, Georgia 30332, USA
J Biomed Opt 10:44025. 2005..Our observation reveals intriguing subcellular associations of mRNA with organelles such as mitochondria, which may provide new insight into the transport, dynamics, and functions of mRNA and mRNA-protein interactions... - Molecular beacons can assess changes in expression and 3'-polyadenylation of human eNOS mRNARachel Jones
Div of Cardiology, Emory Univ School of Medicine, Atlanta, GA 30322, USA
Am J Physiol Cell Physiol 296:C498-504. 2009..This approach could easily be adapted for studies of other endothelial genes and has promise for applications in live endothelial cells... - Use of molecular beacons to image effects of titanium surface microstructure on beta1 integrin expression in live osteoblast-like cellsFrances E Lennon
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332 0363, USA
Biomaterials 31:7640-7. 2010..This technique overcomes the limitations of traditional gene assays (PCR, immunofluorescence) by allowing for the real-time measurement and tracking of specific mRNAs in individual live cells prior to confluence... - Slow non-specific accumulation of 2'-deoxy and 2'-O-methyl oligonucleotide probes at mitochondria in live cellsWon Jong Rhee
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Nucleic Acids Res 38:e109. 2010..These results may help design and optimize fluorescence imaging probes for long-time RNA detection and monitoring in living cells... - HuR regulates the expression of stress-sensitive genes and mediates inflammatory response in human umbilical vein endothelial cellsWon Jong Rhee
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Proc Natl Acad Sci U S A 107:6858-63. 2010..Taken together, these results suggest that HuR plays a critical role in inducing inflammatory response of endothelial cells under mechanical and biochemical stresses... - Coating optimization of superparamagnetic iron oxide nanoparticles for high T2 relaxivitySheng Tong
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, United States
Nano Lett 10:4607-13. 2010..With 14 nm core and PEG1000 coating, SPIOs can have T2 relaxivity of 385 s-1 mM-1, which is among the highest per-Fe atom relaxivities. In vivo tumor imaging results demonstrated the potential of the SPIOs for clinical applications... - Dynamics of filamentous viral RNPs prior to egressPhilip J Santangelo
Wallace H Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, GA 30332, USA
Nucleic Acids Res 35:3602-11. 2007.... - Live-cell characterization and analysis of a clinical isolate of bovine respiratory syncytial virus, using molecular beaconsPhilip Santangelo
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Dr, Atlanta, GA 30332, USA
J Virol 80:682-8. 2006..Taken together, the use of molecular beacons for active virus imaging provides a powerful tool for rapid viral infection detection, the characterization of RNA viruses, and the design of new antiviral drugs... - NLS peptide conjugated molecular beacons for visualizing nuclear RNA in living cellsNitin Nitin
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
Bioconjug Chem 19:2205-11. 2008..The results were validated using in situ hybridization as positive control and random beacons as negative control. This novel approach may be applied to imaging other nuclear RNAs and pre-mRNAs in living cells... - MicroRNA Expression Profile in CAD Patients and the Impact of ACEI/ARBMartina Weber
Division of Cardiology, Emory University School of Medicine, Atlanta, GA 30322, USA
Cardiol Res Pract 2011:532915. 2011..Importantly, medication use may significantly alter miRNA expression. These findings may have significant implications for identifying and managing individuals that either have CAD or are at risk of developing the disease... - A filtration-based protein microarray techniqueYangqing Xu
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
Anal Chem 75:5345-51. 2003..The potential clinical applications of the filtration-based protein microarrays were demonstrated by detecting carcinoembryonic antigen in human plasma samples... - Quantum dot-fluorescent protein pairs as novel fluorescence resonance energy transfer probesAllison M Dennis
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 313 Ferst Drive, Atlanta, Georgia 30332, USA
Nano Lett 8:1439-45. 2008..This provides a novel approach to developing QD-based FRET probes for biomedical applications... - Engineering imaging probes and molecular machines for nanomedicineSheng Tong
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Sci China Life Sci 55:843-61. 2012..The challenges in translating nanomedicine approaches to clinical applications are discussed... - Shedding light on health and disease using molecular beaconsAndrew Tsourkas
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA
Brief Funct Genomic Proteomic 1:372-84. 2003..The aim of this paper is to foster the development of new molecular beacon-based assays and to stimulate the application of this technology in laboratory and clinical studies of health and disease... - Coating thickness of magnetic iron oxide nanoparticles affects R2 relaxivityLeslie E W LaConte
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA
J Magn Reson Imaging 26:1634-41. 2007..To evaluate the effect of coating thickness on the relaxivity of iron oxide nanoparticles... - Structure-function relationships of shared-stem and conventional molecular beaconsAndrew Tsourkas
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 315 Ferst Drive, Suite 2306, Atlanta, GA 30332, USA
Nucleic Acids Res 30:4208-15. 2002..These findings should enable better design of molecular beacons for various applications... - DNA functionalized single-walled carbon nanotubes for electrochemical detectionChenguo Hu
Department of Applied Physics, Chongqing University, Chongqing 400044, China
J Phys Chem B 109:20072-6. 2005..This was demonstrated by highly selective and sensitive detection of a low concentration of dopamine in the presence of excess ascorbic acid... - An in vitro uniaxial stretch model for axonal injuryBryan J Pfister
Department of Neurosurgery, University of Pennsylvania, Philadelphia, PA, USA
Ann Biomed Eng 31:589-98. 2003..This device can be used to investigate a wide range of biomechanical issues involved in diffuse axonal injury...
Research Grants
- Nanomachines for RNA Synthesis and DNA Repair (RMI)Gang Bao; Fiscal Year: 2004..The detailed plan for the GNC will be developed from results of this workshop and also through further preliminary studies that couple nanotechnology tools and models with the selected biological phenomena. ..
- In vivo gene detection for cancer analysisGang Bao; Fiscal Year: 2007....
- Nanotechnology: Detection & Analysis of Plaque FormationGang Bao; Fiscal Year: 2007..In additional to basic knowledge on cardiology, this PEN is expected to yield methodologies that can direct impact on clinical practice of early detection, diagnosis and treatment of atherosclerosis. ..
- Nanomedicine Center for Nucleoprotein MachinesGang Bao; Fiscal Year: 2007..Our long-term vision is to provide genetic cures for common human diseases based on the ability to manipulate the somatic human genome using Nanomedicine. ..