Research Topics
Genomes and Genes
| Anita SidhuSummaryAffiliation: Georgetown University Country: USA Publications
Research Grants
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Detail Information
Publications
Does alpha-synuclein modulate dopaminergic synaptic content and tone at the synapse?Anita Sidhu
Department of Pediatrics, Georgetown University, Washington, DC, USA
FASEB J 18:637-47. 2004....
Region-specific tauopathy and synucleinopathy in brain of the alpha-synuclein overexpressing mouse model of Parkinson's diseaseTiffany Kaul
Department of Biochemistry and Molecular and Cell Biology, University of California San Diego, La Jolla, California, USA
BMC Neurosci 12:79. 2011..The current studies were undertaken to analyze the distribution of tauopathy in different brain regions in a widely used mouse model of PD, the α-Syn overexpressing mouse...- alpha-Synuclein regulation of the dopaminergic transporter: a possible role in the pathogenesis of Parkinson's diseaseAnita Sidhu
Laboratory of Molecular Neurochemistry, Department of Pediatrics, Georgetown University, The Research Building, Room W222, 3970 Reservoir Road, NW, Washington, DC 20007, USA
FEBS Lett 565:1-5. 2004.... - The role of alpha-synuclein in both neuroprotection and neurodegenerationAnita Sidhu
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Ann N Y Acad Sci 1035:250-70. 2004.... - Hyperphosphorylated Tau in an α-synuclein-overexpressing transgenic model of Parkinson's diseaseThomas Haggerty
Laboratory of Molecular Neurochemistry, Department of Biochemistry and Molecular and Cell Biology, Georgetown University Medical Center, The New Research Building, Room W222, Washington, DC 20007, USA
Eur J Neurosci 33:1598-610. 2011..The elevated state of tauopathy seen in these platelet-derived growth factor-α-synuclein mice provides further confirmation that PD may be a tauopathic disease... - Modulation of dopamine transporter function by alpha-synuclein is altered by impairment of cell adhesion and by induction of oxidative stressChristophe Wersinger
Department of Pediatrics, Georgetown University, Washington, DC, USA
FASEB J 17:2151-3. 2003..These studies suggest a novel normative role for alpha-synuclein in regulating DA synaptic availability and homeostasis, which is relevant to the pathophysiology of PD... - Mutations in the lipid-binding domain of alpha-synuclein confer overlapping, yet distinct, functional properties in the regulation of dopamine transporter activityChristophe Wersinger
Department of Pediatrics, Georgetown University Medical Center, 3870 Reservoir Road NW, Washington, DC 20007, USA
Mol Cell Neurosci 24:91-105. 2003.... - Dopamine promotes striatal neuronal apoptotic death via ERK signaling cascadesJun Chen
Department of Pediatrics, Georgetown University, Washington DC 20007, USA
Eur J Neurosci 29:287-306. 2009..These data provide unique insight into a novel role of p-ERK in striatal neurodegeneration... - Trypsin disrupts the trafficking of the human dopamine transporter by alpha-synuclein and its A30P mutantChristophe Wersinger
Department of Pediatrics, Georgetown University, Washington, D.C. 20007, USA
Biochemistry 43:1242-53. 2004..These results show that alpha-synuclein modulates hDAT function through trafficking of the transporter in a process that can be disrupted by trypsin... - Bimodal induction of dopamine-mediated striatal neurotoxicity is mediated through both activation of D1 dopamine receptors and autoxidationChristophe Wersinger
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Mol Cell Neurosci 25:124-37. 2004..The reduction of DA striatal neurotoxicity through blockade of D1 DA receptors, suggests novel therapeutic approaches in the management of striatal neurodegeneration... - Differential cytotoxicity of human wild type and mutant alpha-synuclein in human neuroblastoma SH-SY5Y cells in the presence of dopamineCharbel E-H Moussa
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Biochemistry 43:5539-50. 2004..These findings suggest a specific sequence of events triggered by dopamine and differentially exacerbated by alpha-synuclein and the A30P mutant... - Chronic stimulation of D1 dopamine receptors in human SK-N-MC neuroblastoma cells induces nitric-oxide synthase activation and cytotoxicityJun Chen
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
J Biol Chem 278:28089-100. 2003..These data demonstrate a novel pathway of dopamine-mediated postsynaptic oxidative stress and cell death through direct activation of NOS enzymes by D1 dopamine receptors and its associated signaling pathways... - Partial regulation of serotonin transporter function by gamma-synucleinChristophe Wersinger
Department of Biochemistry, Georgetown University, Washington, DC 20007, USA
Neurosci Lett 453:157-61. 2009..These studies document a novel physiological role for gamma-Syn in regulating 5-HT synaptic availability and homeostasis, and may be of relevance in depression and mood disorders, where SERT function is dysregulated... 
Desipramine modulation of alpha-, gamma-synuclein, and the norepinephrine transporter in an animal model of depressionALEXIS M JEANNOTTE
Department of Biochemistry and Molecular and Cell Biology, Georgetown University Medical Center, Georgetown University, Washington, DC, USA
Neuropsychopharmacology 34:987-98. 2009..These results are the first to implicate alpha-Syn or gamma-Syn in the pathophysiology of depression and suggest that targeting synucleins may provide a new therapeutic option for depression...- Elevated tauopathy and alpha-synuclein pathology in postmortem Parkinson's disease brains with and without dementiaJonathan Wills
Department of Biochemistry and Molecular and Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
Exp Neurol 225:210-8. 2010.... - Alterations of striatal glutamate transmission in rotenone-treated mice: MRI/MRS in vivo studiesCharbel E H Moussa
Laboratory of Molecular Neurochemistry, Department of Biochemistry, Molecular and Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
Exp Neurol 209:224-33. 2008..These studies provide novel insights on the effects of environmental toxins on glutamate and other amino acid neurotransmitters in human neurodegenerative diseases... - The neurotoxin, MPP+, induces hyperphosphorylation of Tau, in the presence of alpha-Synuclein, in SH-SY5Y neuroblastoma cellsTetyana Duka
Department of Biochemistry and Molecular and Cellular Biology, Georgetown University, Washington DC 20007, USA
Neurotox Res 10:1-10. 2006..These data indicate that alpha-Syn and p-Tau modulate the pathogenicity of one another, suggesting a novel convergent mechanism of neurodegeneration... - Modulation of the trafficking of the human serotonin transporter by human alpha-synucleinChristophe Wersinger
Department of Biochemistry, Molecular and Cell Biology, Georgetown University, The Research Building, Room W222, 3970 Reservoir Road, NW, Washington, DC 20007, USA
Eur J Neurosci 24:55-64. 2006..These studies suggest a novel physiological role for alpha-Syn in regulating SERT activity and may be of relevance in certain mental illnesses and in depression, in which SERT function is believed to be dysregulated... - Alpha-synuclein induces hyperphosphorylation of Tau in the MPTP model of parkinsonismTetyana Duka
Department of Biochemistry, Molecular and Cellular Biology, Georgetown University, Washington, DC, USA
FASEB J 20:2302-12. 2006.... - Alpha-Synuclein contributes to GSK-3beta-catalyzed Tau phosphorylation in Parkinson's disease modelsTetyana Duka
Department of Biochemistry, Molecular and Cellular Biology, Georgetown University, Washington, District of Columbia, USA
FASEB J 23:2820-30. 2009..These data unveil a previously unappreciated role of alpha-Syn in the induction of p-GSK-3beta, and demonstrate the importance of this kinase in the genesis and maintenance of neurodegenerative changes associated with PD... - Regulated interactions of the norepineprhine transporter by the actin and microtubule cytoskeletonsALEXIS M JEANNOTTE
Interdisciplinary Program in Neuroscience, Department of Biochemistry and Molecular and Cell Biology, Georgetown University, Washington, DC 20007, USA
J Neurochem 105:1668-82. 2008..This is the first direct demonstration of NET activity being regulated via actin and modulated by interactions with alpha-Syn... - Tauopathic changes in the striatum of A53T α-synuclein mutant mouse model of Parkinson's diseaseJonathan Wills
Department of Biochemistry, Molecular and Cellular Biology, Georgetown University, Washington, DC, United States of America
PLoS ONE 6:e17953. 2011..Together, these data demonstrate an elevated state of tauopathy in striata of the A53T α-Syn mutant mice, suggesting that tauopathy is a common feature of synucleinopathies... - Dopamine differentially induces aggregation of A53T mutant and wild type alpha-synuclein: insights into the protein chemistry of Parkinson's diseaseCharbel E H Moussa
Department of Biochemistry, Molecular and Cell Biology, and Georgetown University, Washington, DC 20007, USA
Biochem Biophys Res Commun 365:833-9. 2008..The present data indicate that mutation in the alpha-synuclein gene may predispose the protein to dopamine-induced aggregation, thereby contributing to disease pathogenesis... - Desipramine induced changes in the norepinephrine transporter, alpha- and gamma-synuclein in the hippocampus, amygdala and striatumALEXIS M JEANNOTTE
Department of Biochemistry and Molecular and Cell Biology, Georgetown University Medical Center, Georgetown University, Washington, DC 20007, United States
Neurosci Lett 467:86-9. 2009.... - D1 dopamine receptor mediates dopamine-induced cytotoxicity via the ERK signal cascadeJun Chen
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
J Biol Chem 279:39317-30. 2004..These data provide novel mechanistic insights into the role of ERK in the cytotoxicity mediated upon activation of the D1 dopamine receptor... - Attenuation of the norepinephrine transporter activity and trafficking via interactions with alpha-synucleinChristophe Wersinger
Department of Biochemistry, Molecular and Cell Biology, Georgetown University, Washington, DC 20007, USA
Eur J Neurosci 24:3141-52. 2006.... - Dopamine D1 receptor-mediated toxicity in human SK-N-MC neuroblastoma cellsCharbel E-H Moussa
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Neurochem Int 48:226-34. 2006..The present studies provide novel insight into a possible patho-physiological staging of cytotoxic events that are mediated by activation of D(1) receptor... - Mice expressing the A53T mutant form of human alpha-synuclein exhibit hyperactivity and reduced anxiety-like behaviorDianca R Graham
Department of Biochemistry, Molecular and Cellular Biology, Georgetown University, Washington, DC 20007, USA
J Neurosci Res 88:1777-83. 2010..These results indicate a possible role of the A53T alpha-Syn mutation in anxiety-like and hyperactive behaviors in a PD mouse model, suggesting that these behaviors might be comorbid with this disease... - Regulation of the norepinephrine transporter by alpha-synuclein-mediated interactions with microtubulesALEXIS M JEANNOTTE
Department of Biochemistry and Molecular and Cell Biology, Georgetown University, Washington, DC 20007, USA
Eur J Neurosci 26:1509-20. 2007..These findings suggest that the surface localization and activity of NET is modulated by alpha-Syn in a manner that is both dependent on interactions with the MT cytoskeleton and varies across brain regions... - Attenuation of dopamine transporter activity by alpha-synucleinChristophe Wersinger
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Neurosci Lett 340:189-92. 2003..Thus, under normal (non-toxic) expression conditions, alpha-synuclein negatively modulates dopamine uptake by DAT... - Abnormal migration of human wild-type alpha-synuclein upon gel electrophoresisCharbel E-H Moussa
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Neurosci Lett 371:239-43. 2004..These studies raise important considerations about the type of protein gel electrophoresis system suitable to study the alterations of alpha-synuclein associated with neurodegeneration, PD and other synucleinopathies... - Differential cytotoxicity of dopamine and H2O2 in a human neuroblastoma divided cell line transfected with alpha-synuclein and its familial Parkinson's disease-linked mutantsChristophe Wersinger
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Neurosci Lett 342:124-8. 2003..These results show an absence of protective effects for the A30P/A53T mutants, and a differential cytoprotective role of alpha-synuclein against oxidants, which varies according to expression levels... - Disruption of the interaction of alpha-synuclein with microtubules enhances cell surface recruitment of the dopamine transporterChristophe Wersinger
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Biochemistry 44:13612-24. 2005.... - Comparative analyses of alpha-synuclein expression levels in rat brain tissues and transfected cellsChristophe Wersinger
Department of Pediatrics, Georgetown University, Washington, DC 20007, USA
Neurosci Lett 358:95-8. 2004.... - Inflammation and Parkinson's diseaseChristophe Wersinger
Laboratory of Molecular Neurochemistry, Department of Pediatrics, Georgetown University Medical Center, 3970 Reservoir Road, NW, Washington, DC 20007, USA
Curr Drug Targets Inflamm Allergy 1:221-42. 2002..The major similarities and differences between PD and other neurodegenerative disorders are discussed... - An inflammatory pathomechanism for Parkinson's disease?Christophe Wersinger
Laboratory of Molecular Neurochemistry, Department of Pediatrics, Georgetown University Medical Center, 3970 Reservoir Road, NW, Washington, DC 20007, USA
Curr Med Chem 13:591-602. 2006..The current status of anti-inflammatory agents, either already tried in PD clinical trials or putatively usefull as adjuvant therapies for PD, is also discussed... - Synuclein modulation of monoamine transportersAdam W Oaks
Department of Biochemistry, Molecular, Cellular Biology and Lombardi Cancer Center, Georgetown University, Washington, DC 20007, United States
FEBS Lett 585:1001-6. 2011.... - Modulation of D1-like dopamine receptor function by aldehydic products of lipid peroxidationYangmee Shin
Department of Pediatrics, Georgetown University Medical Center, 3970 Reservoir Road, N.W, 20057, Washington, DC, USA
Brain Res 968:102-13. 2003..Our data suggests that aldehydic products of lipid peroxidation can directly modulate the binding and functional properties of D1/D5 receptors, as well as effector proteins within their signaling pathway... - The degeneration of dopamine neurons in Parkinson's disease: insights from embryology and evolution of the mesostriatocortical systemPhilippe Vernier
Development, Evolution, Plasticity of the Nervous System, Institute of Neurobiology A Fessard, CNRS, Gif sur Yvette, France
Ann N Y Acad Sci 1035:231-49. 2004.... - Delineation of the conserved functional properties of D1A, D1B and D1C dopamine receptor subtypes in vertebratesStephane Le Crom
Developpement, Evolution, Plasticité du Système Nerveux UPR2197, Institut de Neurobiologie Alfred Fessard, CNRS, Gif sur Yvette, 91198, France
Biol Cell 96:383-94. 2004..These properties have been instrumental to adapt dopamine system to the physiology of the numerous neuronal networks and functions they control in the large and complex brains of vertebrates... - Highly potent and specific GSK-3beta inhibitors that block tau phosphorylation and decrease alpha-synuclein protein expression in a cellular model of Parkinson's diseaseAlan P Kozikowski
Drug Discovery Program, College of Pharmacy, University of Illinois at Chicago, 833 S Wood St, Chicago, Illinois 60612, USA
ChemMedChem 1:256-66. 2006....
Research Grants
- Dopamine and Oxidative Stress in Parkinson's Disease.Anita Sidhu; Fiscal Year: 2006....
- Gamma-Synuclein-Mediated Regulation of Norepinephrine TransporterAnita Sidhu; Fiscal Year: 2010..This grant is also relevant for the ARRA in that it will immediately create 3 new professional jobs. ..
- Modulation of Serotonergic TransportersAnita Sidhu; Fiscal Year: 2007..The identification of a-synuclein as a regulatory protein partner of SERT holds promise in the development of novel therapeutic strategies in the treatment of depression and drug addiction. ..
- SYNUCLEOPATHIES IN NEURODEGENERATIONAnita Sidhu; Fiscal Year: 2006..Studies will be conducted to determine if changes in alpha-synuclein/DAT interactions will reduce oxidative stress and cell death. ..
- Mechanisms of Tauopathies and SynucleopathiesAnita Sidhu; Fiscal Year: 2007..From such studies, it will be possible to understand the overlapping pathology and mechanisms of tauopathies and synucleopathies, and to develop common targeted therapies. ..
- Modulation of Serotonergic TransportersAnita Sidhu; Fiscal Year: 2010..The identification of a-synuclein as a regulatory protein partner of SERT holds promise in the development of novel therapeutic strategies in the treatment of depression and drug addiction. ..
- Mechanisms of Tauopathies and SynucleopathiesAnita Sidhu; Fiscal Year: 2010..From such studies, it will be possible to understand the overlapping pathology and mechanisms of tauopathies and synucleopathies, and to develop common targeted therapies. ..
- DOPAMINERGIC SIGNALING IN NEUROLOGICAL DISORDERSAnita Sidhu; Fiscal Year: 2005....
- REGULATION OF DOPAMINE RECEPTOR FUNCTION AND EXPRESSIONAnita Sidhu; Fiscal Year: 1999..The information obtained after tetanic stimulation of cells will be correlated with the results obtained from biochemical studies, and will provide unique insight to the DA-mediated processes underlying neuronal cell firing. ..
- DOPAMINERGIC SYSTEMS IN HYPERTENSIVEAnita Sidhu; Fiscal Year: 2003..It is likely that RAS blockers will normalize the redox state of the SHR kidney, and that upon such normalization, Dl DA receptor function may also be normalized, restoring the ability of DA and the Dl DA receptors to excrete sodium. ..