V Papadopoulos

Summary

Affiliation: Georgetown University
Country: USA

Publications

  1. ncbi request reprint Translocator protein (18kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function
    Vassilios Papadopoulos
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Trends Pharmacol Sci 27:402-9. 2006
  2. ncbi request reprint Peripheral-type benzodiazepine receptor in neurosteroid biosynthesis, neuropathology and neurological disorders
    V Papadopoulos
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Northwest, Washington, DC 20057, USA
    Neuroscience 138:749-56. 2006
  3. ncbi request reprint Is there a mitochondrial signaling complex facilitating cholesterol import?
    Vassilios Papadopoulos
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, 3900 Reservoir Road, NW, Washington, DC 20057, USA
    Mol Cell Endocrinol 265:59-64. 2007
  4. ncbi request reprint Peripheral benzodiazepine receptor: structure and function in health and disease
    V Papadopoulos
    Division of Hormone Research, Department of Cell Biology, Pharmacology and Neurosciences, Georgetown University School of Medicine, Med Dent Building, 3900 Reservoir Road, NW, Washington, DC 20057, USA
    Ann Pharm Fr 61:30-50. 2003
  5. ncbi request reprint In search of the function of the peripheral-type benzodiazepine receptor
    V Papadopoulos
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, District of Columbia 20057, USA
    Endocr Res 30:677-84. 2004
  6. ncbi request reprint Molecular cloning, chromosomal localization of human peripheral-type benzodiazepine receptor and PKA regulatory subunit type 1A (PRKAR1A)-associated protein PAP7, and studies in PRKAR1A mutant cells and tissues
    Jun Liu
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    FASEB J 17:1189-91. 2003
  7. ncbi request reprint Targeted disruption of the peripheral-type benzodiazepine receptor gene inhibits steroidogenesis in the R2C Leydig tumor cell line
    V Papadopoulos
    Department of Cell Biology, Georgetown University Medical Center, Washington, D C 20007, USA
    J Biol Chem 272:32129-35. 1997
  8. ncbi request reprint Peripheral benzodiazepine receptor in cholesterol transport and steroidogenesis
    V Papadopoulos
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Steroids 62:21-8. 1997
  9. ncbi request reprint Identification, localization, and function in steroidogenesis of PAP7: a peripheral-type benzodiazepine receptor- and PKA (RIalpha)-associated protein
    H Li
    Division of Hormone Research, Department of Cell Biology, Georgetown University School of Medicine, Washington, DC 20007, USA
    Mol Endocrinol 15:2211-28. 2001
  10. pmc Cholesterol binding at the cholesterol recognition/ interaction amino acid consensus (CRAC) of the peripheral-type benzodiazepine receptor and inhibition of steroidogenesis by an HIV TAT-CRAC peptide
    H Li
    Division of Hormone Research, Departments of Cell Biology and Pharmacology and Neuroscience, Georgetown University School of Medicine, Washington, DC. 20007, USA
    Proc Natl Acad Sci U S A 98:1267-72. 2001

Research Grants

  1. BENEFICIAL EFFECTS OF GINKGO BILOBA IN CANCER
    Vassilios Papadopoulos; Fiscal Year: 2002
  2. Plasma Diagnostic for Alzheimer's Disease Pathology
    Vassilios Papadopoulos; Fiscal Year: 2004
  3. Fetal Origin of Male Reproductive Disorders
    Vassilios Papadopoulos; Fiscal Year: 2006
  4. ACUTE HORMONAL REGULATION OF STEROIDOGENESIS
    Vassilios Papadopoulos; Fiscal Year: 2007
  5. Role of Benzodiazepine Receptor in Leydig Cell Function
    Vassilios Papadopoulos; Fiscal Year: 2007

Collaborators

Detail Information

Publications70

  1. ncbi request reprint Translocator protein (18kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function
    Vassilios Papadopoulos
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Trends Pharmacol Sci 27:402-9. 2006
    ..Translocator protein (18kDa) is proposed as a new name, regardless of the subcellular localization of the protein...
  2. ncbi request reprint Peripheral-type benzodiazepine receptor in neurosteroid biosynthesis, neuropathology and neurological disorders
    V Papadopoulos
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Northwest, Washington, DC 20057, USA
    Neuroscience 138:749-56. 2006
    ..In conclusion, changes in peripheral-type benzodiazepine receptor and neurosteroid levels are part of the phenotype seen in neuropathology and neurological disorders and offer potential targets for new therapies...
  3. ncbi request reprint Is there a mitochondrial signaling complex facilitating cholesterol import?
    Vassilios Papadopoulos
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, 3900 Reservoir Road, NW, Washington, DC 20057, USA
    Mol Cell Endocrinol 265:59-64. 2007
    ....
  4. ncbi request reprint Peripheral benzodiazepine receptor: structure and function in health and disease
    V Papadopoulos
    Division of Hormone Research, Department of Cell Biology, Pharmacology and Neurosciences, Georgetown University School of Medicine, Med Dent Building, 3900 Reservoir Road, NW, Washington, DC 20057, USA
    Ann Pharm Fr 61:30-50. 2003
    ..The role for PBR in these pathological conditions remains to be elucidated. paralleled the elevated neurosteroid synthesis observed in specific brain areas. The role for PBR in these pathological conditions remains to be elucidated...
  5. ncbi request reprint In search of the function of the peripheral-type benzodiazepine receptor
    V Papadopoulos
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, District of Columbia 20057, USA
    Endocr Res 30:677-84. 2004
    ..In other tissues, PBR expression might be part of the mitochondrial membrane biogenesis process involved in increased cell proliferation (cancer, gliosis) and tissue repair (nerve damage and ischemia-reperfusion injury)...
  6. ncbi request reprint Molecular cloning, chromosomal localization of human peripheral-type benzodiazepine receptor and PKA regulatory subunit type 1A (PRKAR1A)-associated protein PAP7, and studies in PRKAR1A mutant cells and tissues
    Jun Liu
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    FASEB J 17:1189-91. 2003
    ....
  7. ncbi request reprint Targeted disruption of the peripheral-type benzodiazepine receptor gene inhibits steroidogenesis in the R2C Leydig tumor cell line
    V Papadopoulos
    Department of Cell Biology, Georgetown University Medical Center, Washington, D C 20007, USA
    J Biol Chem 272:32129-35. 1997
    ..These data demonstrate that PBR is an indispensable element of the steroidogenic machinery, where it mediates the delivery of the substrate cholesterol to the inner mitochondrial side chain cleavage cytochrome P-450...
  8. ncbi request reprint Peripheral benzodiazepine receptor in cholesterol transport and steroidogenesis
    V Papadopoulos
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Steroids 62:21-8. 1997
    ....
  9. ncbi request reprint Identification, localization, and function in steroidogenesis of PAP7: a peripheral-type benzodiazepine receptor- and PKA (RIalpha)-associated protein
    H Li
    Division of Hormone Research, Department of Cell Biology, Georgetown University School of Medicine, Washington, DC 20007, USA
    Mol Endocrinol 15:2211-28. 2001
    ..PAP7 may function by targeting the PKA isoenzyme to organelles rich in PBR, i.e. mitochondria, where phosphorylation of specific protein substrates may induce the reorganization of PBR topography and function...
  10. pmc Cholesterol binding at the cholesterol recognition/ interaction amino acid consensus (CRAC) of the peripheral-type benzodiazepine receptor and inhibition of steroidogenesis by an HIV TAT-CRAC peptide
    H Li
    Division of Hormone Research, Departments of Cell Biology and Pharmacology and Neuroscience, Georgetown University School of Medicine, Washington, DC. 20007, USA
    Proc Natl Acad Sci U S A 98:1267-72. 2001
    ....
  11. ncbi request reprint Structure and function of the peripheral-type benzodiazepine receptor in steroidogenic cells
    V Papadopoulos
    Department of Cell Biology and Pharmacology, Georgetown University Medical Center, Washington, DC 20007, USA
    Proc Soc Exp Biol Med 217:130-42. 1998
    ....
  12. ncbi request reprint Drug-induced inhibition of the peripheral-type benzodiazepine receptor expression and cell proliferation in human breast cancer cells
    V Papadopoulos
    Department of Cell Biology and Pharmacology, Georgetown University Medical Center, Washington, DC 20007, USA
    Anticancer Res 20:2835-47. 2000
    ..Taken together, these data suggest that the manipulation of PBR expression could be used to control tumor growth and that EGb 761 and GKB, under the conditions used, exert cytostatic properties...
  13. ncbi request reprint Structure, function and regulation of the mitochondrial peripheral-type benzodiazepine receptor
    V Papadopoulos
    Division of Hormone Research, Departments of Cell Biology, Pharmacology and Neuroscience, Georgetown University School of Medicine, Washington, DC 20007, USA
    Therapie 56:549-56. 2001
    ....
  14. ncbi request reprint PBR, StAR, and PKA: partners in cholesterol transport in steroidogenic cells
    T Hauet
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, 3900 Reservoir Road, Washington, DC 20057, USA
    Endocr Res 28:395-401. 2002
    ..These data indicate that hormone-induced cholesterol transport and the subsequent steroid formation is a dynamic multistep process involving protein-protein interactions...
  15. ncbi request reprint Effect of mono-ethylhexyl phthalate on MA-10 Leydig tumor cells
    J H Dees
    Division of Hormone Research, Department of of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Reprod Toxicol 15:171-87. 2001
    ..5-fold over control levels at MEHP concentrations of 10(-6) to 10(-3) M whereas mitochondrial volume fraction decreased. These results suggest that MEHP in Leydig cells may act as a mitochondrial toxicant and lipid metabolism disrupter...
  16. ncbi request reprint Inhibition of adrenal cortical steroid formation by procaine is mediated by reduction of the cAMP-induced 3-hydroxy-3-methylglutaryl-coenzyme A reductase messenger ribonucleic acid levels
    Jing Xu
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, 3900 Reservoir Road, Washington, DC 20057, USA
    J Pharmacol Exp Ther 307:1148-57. 2003
    ..Taken together, these results suggest that procaine may provide a pharmacological means for the control of hormone-induced HMG-CoA reductase mRNA expression and hypercortisolemia...
  17. ncbi request reprint Effect of peroxisome proliferators on Leydig cell peripheral-type benzodiazepine receptor gene expression, hormone-stimulated cholesterol transport, and steroidogenesis: role of the peroxisome proliferator-activator receptor alpha
    Maria Gazouli
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Endocrinology 143:2571-83. 2002
    ..These results suggest that the antiandrogenic effect of PPs is mediated by a PPARalpha-dependent inhibition of Leydig cell PBR gene expression...
  18. ncbi request reprint 22R-Hydroxycholesterol induces differentiation of human NT2 precursor (Ntera2/D1 teratocarcinoma) cells
    Z X Yao
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Neuroscience 148:441-53. 2007
    ..However, using a cholesterol protein binding blot assay we demonstrated the presence of a 22R-hydroxycholesterol-binding protein in NT2 cells distinct from the human oxysterol receptors liver X receptor LXRalpha and beta...
  19. pmc Drug ligand-induced activation of translocator protein (TSPO) stimulates steroid production by aged brown Norway rat Leydig cells
    J Y Chung
    Department of Biochemistry and Molecular Biology, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205, USA
    Endocrinology 154:2156-65. 2013
    ..We suggest that this approach might serve as a therapeutic means to increase steroid levels in vivo in cases of primary hypogonadism...
  20. ncbi request reprint Peripheral-type benzodiazepine receptor levels correlate with the ability of human breast cancer MDA-MB-231 cell line to grow in SCID mice
    M Hardwick
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Int J Cancer 94:322-7. 2001
    ..Only the subclone with high PBR levels, however, was able to form tumors when injected in SCID mice. These data suggest that the ability of MDA-231 cells to form tumors in vivo may depend on the amount of PBR present in the cells...
  21. pmc In utero exposure to di-(2-ethylhexyl) phthalate decreases mineralocorticoid receptor expression in the adult testis
    D B Martinez-Arguelles
    Department of Biochemistry, Georgetown University Medical Center, Washington, DC 20057, USA
    Endocrinology 150:5575-85. 2009
    ..We suggest that decreased MR, possibly epigenetically mediated, is a novel mechanism by which phthalates may affect diverse functions later in life...
  22. ncbi request reprint Role of the peripheral-type benzodiazepine receptor and the polypeptide diazepam binding inhibitor in steroidogenesis
    V Papadopoulos
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    J Steroid Biochem Mol Biol 53:103-10. 1995
    ..Thus, in vivo, hormonal activation of these two mechanisms results in efficient cholesterol delivery and utilization and thus high levels of steroid synthesis...
  23. ncbi request reprint Peripheral-type benzodiazepine receptor-mediated action of steroidogenic acute regulatory protein on cholesterol entry into leydig cell mitochondria
    Thierry Hauet
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Mol Endocrinol 19:540-54. 2005
    ..These data suggest that both StAR and PBR proteins are indispensable elements of the steroidogenic machinery and function in a coordinated manner to transfer cholesterol into mitochondria...
  24. ncbi request reprint Identification, design, synthesis, and pharmacological activity of (4-ethyl-piperazin-1-yl)-phenylmethanone derivatives with neuroprotective properties against beta-amyloid-induced toxicity
    Laurent Lecanu
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Neuropharmacology 49:86-96. 2005
    ..These data suggest that the identified (4-ethyl-piperaz-1-yl)-phenylmethanone chemical entity exerts neuroprotective properties and may serve as a lead compound for the development of novel therapies for AD...
  25. ncbi request reprint In vitro reconstitution of a functional peripheral-type benzodiazepine receptor from mouse Leydig tumor cells
    M Garnier
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007
    Mol Pharmacol 45:201-11. 1994
    ....
  26. ncbi request reprint Cholesterol transport, peripheral benzodiazepine receptor, and steroidogenesis in aging Leydig cells
    Martine Culty
    Department of Cell Biology, Georgetown University School of Medicine, Washington, DC, USA
    J Androl 23:439-47. 2002
    ..Taken together, these results suggest that alterations in cholesterol transport and in PBR may play critical roles in age-related decreases in testosterone production in Brown Norway rat Leydig cells...
  27. ncbi request reprint Molecular cloning, genomic organization, chromosomal mapping and subcellular localization of mouse PAP7: a PBR and PKA-RIalpha associated protein
    Jun Liu
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, 3900 Reservoir Road, NW, Washington, DC 20057, USA
    Gene 308:1-10. 2003
    ..e. mitochondria, where phosphorylation of specific protein substrates mediates the hormone-induced steroid formation...
  28. ncbi request reprint Mitochondrial peripheral-type benzodiazepine receptor expression. Correlation with gonadotropin-releasing hormone (GnRH) agonist-induced apoptosis in the corpus luteum
    V Papadopoulos
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007 2197, USA
    Biochem Pharmacol 58:1389-93. 1999
    ..Alternatively, PBR may exert an as yet unidentified anti-apoptotic function...
  29. ncbi request reprint Role of the peripheral-type benzodiazepine receptor in adrenal and brain steroidogenesis
    R C Brown
    Division of Hormone Research, Department of Cell Biology, Interdisciplinary Program in Neuroscience, Georgetown University Medical Center, Washington, DC 20007, USA
    Int Rev Neurobiol 46:117-43. 2001
    ....
  30. ncbi request reprint Cholesterol homeostasis and infertility: the liver X receptor connection
    Vassilios Papadopoulos
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, 3900 Reservoir Road Northwest, Washington, DC 20057, USA
    Endocrinology 146:2517-8. 2005
  31. ncbi request reprint Ginkgo biloba extract (Egb 761) inhibits beta-amyloid production by lowering free cholesterol levels
    Zhi Xing Yao
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, 3900 Reservoir Road, NW, Washington, DC 20057, USA
    J Nutr Biochem 15:749-56. 2004
    ..Our findings also provide the first demonstration that EGb 761 can influence these mechanisms...
  32. ncbi request reprint Cutting-edge patents in Alzheimer's disease drug discovery: anticipation of potential future treatments
    Laurent Lecanu
    Department of Biochemistry, Molecular and Cellular Biology, Georgetown University Medical Center, Washington DC, 20057, USA
    Recent Pat CNS Drug Discov 2:113-23. 2007
    ..We hope to provide the reader with a broader and better understanding of what could be new therapies for AD during the next decade...
  33. ncbi request reprint Local anesthetic procaine protects rat pheochromocytoma PC12 cells against beta-amyloid-induced neurotoxicity
    Laurent Lecanu
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Pharmacology 74:65-78. 2005
    ..In conclusion, these data suggest that procaine exerts neuroprotective properties and may serve either as a treatment for AD or as a starting point for the development of novel therapies for AD...
  34. ncbi request reprint PAP7, a PBR/PKA-RIalpha-associated protein: a new element in the relay of the hormonal induction of steroidogenesis
    Jun Liu
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, DC 20057, USA
    J Steroid Biochem Mol Biol 85:275-83. 2003
    ..Taken together, these data suggest that PAP7 functions as an A-kinase anchoring protein (AKAP) critical in the cAMP-dependent steroid formation...
  35. ncbi request reprint Modulation of peripheral-type benzodiazepine receptor levels in a reperfusion injury pig kidney-graft model
    Thierry Hauet
    Unité de Transplantation Expérimentale, Département de Génétique Animale, Institut National de Recherche Agronomique, Domaine du Magneraud, Surgeres, France
    Transplantation 74:1507-15. 2002
    ..Ischemia-reperfusion injury is associated with an increased risk of acute rejection, delayed graft function, or chronic graft dysfunction. Mitochondria play a central role in this process...
  36. ncbi request reprint Function of beta-amyloid in cholesterol transport: a lead to neurotoxicity
    Zhi Xing Yao
    Division of Hormone Research, Department of Cell Biology, Georgetown University School of Medicine, Washington, DC 20057, USA
    FASEB J 16:1677-9. 2002
    ..High cholesterol levels also lead to overproduction of Abeta. Abeta blocks cholesterol trafficking and changes cholesterol homeostasis leading to neurodegeneration and the onset and/or progression of AD pathology...
  37. ncbi request reprint Protein-protein interactions mediate mitochondrial cholesterol transport and steroid biosynthesis
    Jun Liu
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, Washington, D C 20057, USA
    J Biol Chem 281:38879-93. 2006
    ....
  38. ncbi request reprint Identification of a peptide antagonist to the peripheral-type benzodiazepine receptor that inhibits hormone-stimulated leydig cell steroid formation
    Maria Gazouli
    Division of Hormone Research, Departments of Cell Biology, Pharmacology and Neuroscience, Georgetown University Medical Center, 3900 Reservoir Road, Washington, DC 20007, USA
    J Pharmacol Exp Ther 303:627-32. 2002
    ..These results yield leads for the development of potent PBR antagonists and indicate that endogenous PBR agonist-receptor interaction is critical for hormone-induced steroidogenesis...
  39. ncbi request reprint The spirostenol (22R, 25R)-20alpha-spirost-5-en-3beta-yl hexanoate blocks mitochondrial uptake of Abeta in neuronal cells and prevents Abeta-induced impairment of mitochondrial function
    Laurent Tillement
    Department of Biochemistry and Molecular Biology, Georgetown University School of Medicine, Washington, DC 20057, USA
    Steroids 71:725-35. 2006
    ..These results indicate that Abeta(1-42) and SP-233 exert direct effects on mitochondrial function and SP-233 protects neuronal cells against Abeta-induced toxicity by targeting Abeta directly...
  40. ncbi request reprint 22R-Hydroxycholesterol protects neuronal cells from beta-amyloid-induced cytotoxicity by binding to beta-amyloid peptide
    Zhi Xing Yao
    Division of Hormone Research, Departments of Cell Biology, Pharmacology and Neurosciences and Samaritan Research Laboratories, Georgetown University School of Medicine, Washington DC 20057, USA
    J Neurochem 83:1110-9. 2002
    ..We propose that 22R-hydroxycholesterol offers a new means of neuroprotection against Abeta toxicity by inactivating the peptide...
  41. ncbi request reprint Peripheral-type benzodiazepine receptor overexpression and knockdown in human breast cancer cells indicate its prominent role in tumor cell proliferation
    Wenping Li
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Biochem Pharmacol 73:491-503. 2007
    ....
  42. doi request reprint Identification of a benzamide derivative that inhibits stress-induced adrenal corticosteroid synthesis
    Jing Xu
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Molecules 14:3392-410. 2009
    ....
  43. ncbi request reprint A capillary gas chromatography/mass spectrometric method for the quantification of hydroxysteroids in human plasma
    S Diallo
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Anal Biochem 324:123-30. 2004
    ..Under these conditions the method was linear (typical R2 is superior to 0.98 for all hydroxysteroids studied) over the concentration range of 2 x 10(-9) to 10(-6)M with good precision and accuracy...
  44. ncbi request reprint Differential utilization of the promoter of peripheral-type benzodiazepine receptor by steroidogenic versus nonsteroidogenic cell lines and the role of Sp1 and Sp3 in the regulation of basal activity
    Christoforos Giatzakis
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Endocrinology 145:1113-23. 2004
    ....
  45. ncbi request reprint Prenatal exposure of rats to Ginkgo biloba extract (EGb 761) increases neuronal survival/growth and alters gene expression in the developing fetal hippocampus
    Wenping Li
    Department of Cell Biology, Division of Hormone Research, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, DC 20057, USA
    Brain Res Dev Brain Res 144:169-80. 2003
    ..These effects of EGb 761 may underlie its neuroprotective properties...
  46. ncbi request reprint Oxidative stress-mediated DHEA formation in Alzheimer's disease pathology
    Rachel C Brown
    The Interdisciplinary Program in Neuroscience, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, DC 20007, USA
    Neurobiol Aging 24:57-65. 2003
    ..AD brain DHEA is formed by oxidative stress metabolism of precursor, and (iii). CSF DHEA levels and serum DHEA formation in response to FeSO(4) may serve as an indicator of AD pathology...
  47. ncbi request reprint Identification of naturally occurring spirostenols preventing beta-amyloid-induced neurotoxicity
    Laurent Lecanu
    Department of Cell Biology, Georgetown University School of Medicine, Washington, DC 20057, USA
    Steroids 69:1-16. 2004
    ..In conclusion, these results suggest that spirost-5-en-3-ol naturally occurring derivatives of 22R-hydroxycholesterol might offer a new approach for Alzheimer's disease therapy...
  48. ncbi request reprint Cancer-related overexpression of the peripheral-type benzodiazepine receptor and cytostatic anticancer effects of Ginkgo biloba extract (EGb 761)
    Ewald Pretner
    Department of Biochemistry and Molecular Biology, Georgetown University School of Medicine, Washington DC, 20007, USA
    Anticancer Res 26:9-22. 2006
    ..Since EGb 761 treatment opposes this aggressive phenotype by decreasing PBR overexpression, it could be useful in preventing or treating cancer invasiveness and metastasis...
  49. ncbi request reprint Identification of a stimulator of steroid hormone synthesis isolated from testis
    N Boujrad
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Science 268:1609-12. 1995
    ..Thus, a TIMP-1-procathepsin L complex is a potent activator of steroidogenesis and may regulate steroid concentrations and, thus, germ cell development in both males and females...
  50. pmc Epigenetic regulation of the expression of genes involved in steroid hormone biosynthesis and action
    Daniel B Martinez-Arguelles
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Steroids 75:467-76. 2010
    ..Here we review evidence for epigenetic regulation of enzymes, transcription factors, and nuclear receptors related to steroid biogenesis and action...
  51. pmc The role of Ets transcription factors in the basal transcription of the translocator protein (18 kDa)
    Christoforos Giatzakis
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, 3900 Reservoir Road NW, Washington, DC 20057, USA
    Biochemistry 46:4763-74. 2007
    ..We conclude that Sp1/Sp3 and members of the Ets family of transcription factors bind to specific binding sites in the TSPO promoter to drive basal TSPO gene transcription...
  52. pmc Phorbol-12-myristate 13-acetate acting through protein kinase Cepsilon induces translocator protein (18-kDa) TSPO gene expression
    Amani Batarseh
    Department of Biochemistry and Molecular and Cell Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Biochemistry 47:12886-99. 2008
    ....
  53. pmc Targeting and insertion of the cholesterol-binding translocator protein into the outer mitochondrial membrane
    Malena B Rone
    Department of Biochemistry, Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    Biochemistry 48:6909-20. 2009
    ..Final integration of TSPO into the OMM occurs via its interaction with Metaxin 1. Import of TSPO into steroidogenic cell mitochondria is regulated by cAMP...
  54. ncbi request reprint Use of ginkgolide B and a ginkgolide-activated response element to control gene transcription: example of the adrenocortical peripheral-type benzodiazepine receptor
    Hakima Amri
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC, USA
    Cell Mol Biol (Noisy-le-grand) 48:633-9. 2002
    ....
  55. ncbi request reprint Peripheral-type benzodiazepine receptor (PBR) gene amplification in MDA-MB-231 aggressive breast cancer cells
    Matthew Hardwick
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, Georgetown, Washington DC 20007, USA
    Cancer Genet Cytogenet 139:48-51. 2002
    ..These data suggest that PBR gene amplification may be an important indicator of breast cancer progression...
  56. ncbi request reprint Transcriptional suppression of the adrenal cortical peripheral-type benzodiazepine receptor gene and inhibition of steroid synthesis by ginkgolide B
    Hakima Amri
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, 3900 Reservoir Road, Washington, DC 20057, USA
    Biochem Pharmacol 65:717-29. 2003
    ....
  57. ncbi request reprint In vivo and in vitro peripheral-type benzodiazepine receptor polymerization: functional significance in drug ligand and cholesterol binding
    Franck Delavoie
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Biochemistry 42:4506-19. 2003
    ....
  58. ncbi request reprint Haploinsufficiency of cytochrome P450 17alpha-hydroxylase/17,20 lyase (CYP17) causes infertility in male mice
    Ying Liu
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Mol Endocrinol 19:2380-9. 2005
    ....
  59. ncbi request reprint Beta-amyloid and oxidative stress jointly induce neuronal death, amyloid deposits, gliosis, and memory impairment in the rat brain
    Laurent Lecanu
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Pharmacology 76:19-33. 2006
    ....
  60. ncbi request reprint Protoporphyrin IX binding and transport by recombinant mouse PBR
    Gregor Wendler
    Division of Hormone Research, Departments of Cell Biology, Pharmacology and Neurosciences, Georgetown University School of Medicine, 3900 Reservoir Road, NW, Washington, DC, USA
    Biochem Biophys Res Commun 311:847-52. 2003
    ..These results suggest that PBR, in addition to its role in cholesterol and coproporphyrinogen III transport, is also directing the mitochondrial PPIX import, a function that can be ascribed to the 18kDa PBR protein alone...
  61. ncbi request reprint Expression of peripheral benzodiazepine receptor (PBR) in human tumors: relationship to breast, colorectal, and prostate tumor progression
    Zeqiu Han
    Division of Hormone Research, Department of Cell Biology, Georgetown University Medical Center, Washington, District of Columbia 20057, USA
    J Recept Signal Transduct Res 23:225-38. 2003
    ..Thus, we propose that PBR overexpression could serve as a novel prognostic indicator of an aggressive phenotype in breast, colorectal and prostate cancers...
  62. ncbi request reprint Identification of the adrenocorticotropin and ginkgolide B-regulated 90-kilodalton protein (p90) in adrenocortical cells as a serotransferrin precursor protein homolog (adrenotransferrin)
    Hakima Amri
    Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, DC 20057, USA
    Endocrinology 145:1802-9. 2004
    ....
  63. pmc Protein kinase C epsilon regulation of translocator protein (18 kDa) Tspo gene expression is mediated through a MAPK pathway targeting STAT3 and c-Jun transcription factors
    Amani Batarseh
    Department of Biochemistry and Molecular and Cell Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Biochemistry 49:4766-78. 2010
    ..Together, these results demonstrate that PKCepsilon regulates Tspo gene expression through a MAPK (Raf-1-MEK1/2-ERK1/2) signal transduction pathway, acting at least in part through c-Jun and STAT3 transcription factors...
  64. ncbi request reprint 3D QSAR studies of AChE inhibitors based on molecular docking scores and CoMFA
    Nagaraju Akula
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Washington, DC 20057, USA
    Bioorg Med Chem Lett 16:6277-80. 2006
    ..The superimposed CoMFA models on the receptor site of AChE are guiding the design of potential inhibitory structures directed against AChE activity...
  65. doi request reprint In utero exposure to di-(2-ethylhexyl) phthalate exerts both short-term and long-lasting suppressive effects on testosterone production in the rat
    Martine Culty
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, Washington, District of Columbia 20057, USA
    Biol Reprod 78:1018-28. 2008
    ..Taken together, these results demonstrate that in utero DEHP exposure exerts both short-term and long-lasting effects on testicular steroidogenesis that might involve distinct molecular targets in fetal and adult Leydig cells...
  66. ncbi request reprint Cytochrome P450 17alpha hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cells
    Ying Liu
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, 3900 Reservoir Road Northwest, Washington, D C 20057, USA
    Mol Endocrinol 19:1918-31. 2005
    ....
  67. ncbi request reprint Gestational exposure to atrazine: effects on the postnatal development of male offspring
    Brian G Rosenberg
    Division of Reproductive Biology, Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA
    J Androl 29:304-11. 2008
    ....
  68. ncbi request reprint Abnormal morphology of spermatozoa in cytochrome P450 17alpha-hydroxylase/17, 20-lyase (CYP17) deficient mice
    Ying Liu
    Georgetown University Medical Center, Department of Biochemistry and Molecular and Cellular Biology, 3900 Reservoir Road NW, Washington DC, 20057, USA
    J Androl 28:453-60. 2007
    ..These results suggest that CYP17, in addition to its role in androgen formation, is critical for proper mitochondrial architecture and sperm morphology and thus for sperm function and normal fertility...
  69. ncbi request reprint Methodology for multi-site ligand-protein docking identification developed for the optimization of spirostenol inhibition of beta-amyloid-induced neurotoxicity
    Gary L Teper
    Department of Biochemistry and Molecular Biology, Georgetown University Medical Center, Basic Science Bldg 3rd Floor, 3900 Reservoir Road NW, Washington, DC 20057, USA
    Chem Biodivers 2:1571-9. 2005
    ....
  70. ncbi request reprint Common gene targets of Ginkgo biloba extract (EGb 761) in human tumor cells: relation to cell growth
    Wenping Li
    Department of Cell Biology, Georgetown University Medical Center, Washington, DC 20057 USA
    Cell Mol Biol (Noisy-le-grand) 48:655-62. 2002
    ..These results could therefore help elucidate the mechanism of cytostatic action of EGb 761 and identify genes important for tumor growth...

Research Grants5

  1. BENEFICIAL EFFECTS OF GINKGO BILOBA IN CANCER
    Vassilios Papadopoulos; Fiscal Year: 2002
    ..These experiments should demonstrate if EGB 761 could be used to prevent and/or stabilize the progression of cancer. ..
  2. Plasma Diagnostic for Alzheimer's Disease Pathology
    Vassilios Papadopoulos; Fiscal Year: 2004
    ..In this Phase I application we propose to test this hypothesis in a small number of samples obtained from AD and control (non-demented) patients. ..
  3. Fetal Origin of Male Reproductive Disorders
    Vassilios Papadopoulos; Fiscal Year: 2006
    ....
  4. ACUTE HORMONAL REGULATION OF STEROIDOGENESIS
    Vassilios Papadopoulos; Fiscal Year: 2007
    ..Data generated from these studies should test our hypothesis that a mitochondrial cAMP signaling complex (transduceosome) directs and amplifies the effects of cAMP leading to the induction and maintenance of steroidogenesis. ..
  5. Role of Benzodiazepine Receptor in Leydig Cell Function
    Vassilios Papadopoulos; Fiscal Year: 2007
    ....