Radoslav Goldman

Summary

Affiliation: Georgetown University
Country: USA

Publications

  1. pmc Detection of hepatocellular carcinoma using glycomic analysis
    Radoslav Goldman
    Department of Oncology, Georgetown University, Washington, District of Columbia 20057 1469, USA
    Clin Cancer Res 15:1808-13. 2009
  2. pmc Decreased risk of squamous cell carcinoma of the head and neck in users of nonsteroidal anti-inflammatory drugs
    Neda Ahmadi
    Department of Otolaryngology Head and Neck Surgery, Georgetown University Hospital, Washington, DC 20007, USA
    Int J Otolaryngol 2010:424161. 2010
  3. pmc Peptides in low molecular weight fraction of serum associated with hepatocellular carcinoma
    Yanming An
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Road NW, Washington, DC 20057, USA
    Dis Markers 29:11-20. 2010
  4. pmc MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration
    Juraj Lenco
    Georgetown University, Department of Oncology, Lombardi Comprehensive Cancer Center, 3970 Reservoir Rd NW, Washington, DC 20057, USA
    Proteome Sci 10:7. 2012
  5. pmc A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins
    Peter D Burbelo
    Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University Medical Center, Washington, DC 20057, USA
    BMC Biotechnol 5:22. 2005
  6. pmc Candidate markers for the detection of hepatocellular carcinoma in low-molecular weight fraction of serum
    Radoslav Goldman
    Georgetown University, Lombardi Comprehensive Cancer Center, 3970 Reservoir Road Northwest, Washington, DC 20057, USA
    Carcinogenesis 28:2149-53. 2007
  7. pmc Analysis of MALDI-TOF mass spectrometry data for discovery of peptide and glycan biomarkers of hepatocellular carcinoma
    Habtom W Ressom
    Georgetown University, Lombardi Comprehensive Cancer Center, Washington, DC, USA
    J Proteome Res 7:603-10. 2008
  8. pmc Integrated peptide and glycan biomarker discovery using MALDI-TOF mass spectrometry
    Rency S Varghese
    Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA
    Conf Proc IEEE Eng Med Biol Soc 2008:3791-4. 2008
  9. pmc Site-specific glycoforms of haptoglobin in liver cirrhosis and hepatocellular carcinoma
    Petr Pompach
    Department of Oncology, Georgetown University, Washington, DC 20057, USA
    Mol Cell Proteomics 12:1281-93. 2013
  10. pmc Identification of N-glycan serum markers associated with hepatocellular carcinoma from mass spectrometry data
    Zhiqun Tang
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA
    J Proteome Res 9:104-12. 2010

Research Grants

  1. Glycans in Hepatocellular Carcinoma
    Radoslav Goldman; Fiscal Year: 2009
  2. Effects of smoking on ERb and AHR pathway in human lung
    Radoslav Goldman; Fiscal Year: 2004
  3. Proteomic Analysis of Serum in Liver Cancer
    Radoslav Goldman; Fiscal Year: 2010
  4. Proteomic Analysis of Serum in Liver Cancer
    Radoslav Goldman; Fiscal Year: 2007
  5. Optimization of enrichment for MALDI TOF/TOF identification of cancer biomarkers
    Radoslav Goldman; Fiscal Year: 2007
  6. Proteomic Analysis of Serum in Liver Cancer
    Radoslav Goldman; Fiscal Year: 2009
  7. Glycans in Hepatocellular Carcinoma
    Radoslav Goldman; Fiscal Year: 2010

Collaborators

Detail Information

Publications30

  1. pmc Detection of hepatocellular carcinoma using glycomic analysis
    Radoslav Goldman
    Department of Oncology, Georgetown University, Washington, District of Columbia 20057 1469, USA
    Clin Cancer Res 15:1808-13. 2009
    ..The objective of our study was to quantitatively assess N-glycans originating from serum glycoproteins as alternative markers for the detection of HCC...
  2. pmc Decreased risk of squamous cell carcinoma of the head and neck in users of nonsteroidal anti-inflammatory drugs
    Neda Ahmadi
    Department of Otolaryngology Head and Neck Surgery, Georgetown University Hospital, Washington, DC 20007, USA
    Int J Otolaryngol 2010:424161. 2010
    ..This study provides evidence for a significant reduction in the risk of developing HNSCC in users of NSAIDs, and specifically aspirin users...
  3. pmc Peptides in low molecular weight fraction of serum associated with hepatocellular carcinoma
    Yanming An
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Road NW, Washington, DC 20057, USA
    Dis Markers 29:11-20. 2010
    ..These peptides could potentially serve as markers of HCC following additional validation studies; however, association of similar peptides with other diseases and cancers dictates a very cautious approach...
  4. pmc MS/MS library facilitated MRM quantification of native peptides prepared by denaturing ultrafiltration
    Juraj Lenco
    Georgetown University, Department of Oncology, Lombardi Comprehensive Cancer Center, 3970 Reservoir Rd NW, Washington, DC 20057, USA
    Proteome Sci 10:7. 2012
    ..The added value of the workflow is demonstrated in the development of a triplex LC-MRM MS assay used for quantification of peptides potentially associated with the progression of liver disease to hepatocellular carcinoma...
  5. pmc A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins
    Peter D Burbelo
    Lombardi Comprehensive Cancer Center, Department of Oncology, Georgetown University Medical Center, Washington, DC 20057, USA
    BMC Biotechnol 5:22. 2005
    ....
  6. pmc Candidate markers for the detection of hepatocellular carcinoma in low-molecular weight fraction of serum
    Radoslav Goldman
    Georgetown University, Lombardi Comprehensive Cancer Center, 3970 Reservoir Road Northwest, Washington, DC 20057, USA
    Carcinogenesis 28:2149-53. 2007
    ..Further evaluation of these marker candidates for the diagnosis of HCC is needed...
  7. pmc Analysis of MALDI-TOF mass spectrometry data for discovery of peptide and glycan biomarkers of hepatocellular carcinoma
    Habtom W Ressom
    Georgetown University, Lombardi Comprehensive Cancer Center, Washington, DC, USA
    J Proteome Res 7:603-10. 2008
    ..Further evaluation is needed to examine the potential clinical utility of the candidate peptide and glycan markers identified in this study...
  8. pmc Integrated peptide and glycan biomarker discovery using MALDI-TOF mass spectrometry
    Rency S Varghese
    Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA
    Conf Proc IEEE Eng Med Biol Soc 2008:3791-4. 2008
    ..The performance of these markers is evaluated in terms of their ability to distinguish HCC cases from patients with CLD in a blinded validation set...
  9. pmc Site-specific glycoforms of haptoglobin in liver cirrhosis and hepatocellular carcinoma
    Petr Pompach
    Department of Oncology, Georgetown University, Washington, DC 20057, USA
    Mol Cell Proteomics 12:1281-93. 2013
    ..The presence of multiply fucosylated Lewis Y glycoforms of haptoglobin in the disease context could have important functional implications...
  10. pmc Identification of N-glycan serum markers associated with hepatocellular carcinoma from mass spectrometry data
    Zhiqun Tang
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20057, USA
    J Proteome Res 9:104-12. 2010
    ..The method of this study is significant in deriving consistent, accurate, and biological significant glycan marker candidates for hepatocellular carcinoma diagnosis...
  11. ncbi request reprint Enrichment of low molecular weight fraction of serum for MS analysis of peptides associated with hepatocellular carcinoma
    Eduard Orvisky
    Georgetown University, Department of Oncology, Lombardi Comprehensive Cancer Center, Washington, DC 20057 1469, USA
    Proteomics 6:2895-902. 2006
    ..This study shows that enrichment of the low molecular weight fraction of serum facilitates an efficient discovery of peptides that could serve as biomarkers for detection of HCC as well as other diseases...
  12. ncbi request reprint Ant colony optimization for biomarker identification from MALDI-TOF mass spectra
    Habtom W Ressom
    Lombardi Comprehensive Cancer Center, Georgetown Univ, Washington, DC, USA
    Conf Proc IEEE Eng Med Biol Soc 1:4560-3. 2006
    ..The results indicate that the MALDI-TOF technology could provide the means to discover novel biomarkers for HCC...
  13. pmc Exploring site-specific N-glycosylation microheterogeneity of haptoglobin using glycopeptide CID tandem mass spectra and glycan database search
    Kevin Brown Chandler
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University Medical Center, Washington, DC 20007, USA
    J Proteome Res 12:3652-66. 2013
    ..4%, representing 15 distinct glycopeptides on at least three of the four N-linked glycosylation sites. The software, GlycoPeptideSearch, is available for download from http://edwardslab.bmcb.georgetown.edu/GPS . ..
  14. pmc Quantitative liquid chromatography-mass spectrometry-multiple reaction monitoring (LC-MS-MRM) analysis of site-specific glycoforms of haptoglobin in liver disease
    Miloslav Sanda
    Department of Oncology, Georgetown University, Washington, DC 20057, USA
    Mol Cell Proteomics 12:1294-305. 2013
    ..The exoglycosidase-assisted LC-MS-MRM workflow, optimized for the quantification of fucosylated glycoforms of haptoglobin, can be used for quantification of these glycoforms on other glycopeptides with appropriate analytical behavior...
  15. ncbi request reprint Analysis of mass spectral serum profiles for biomarker selection
    Habtom W Ressom
    Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA
    Bioinformatics 21:4039-45. 2005
    ..We present low-level methods for the processing of mass spectral data and a machine learning method that combines support vector machines, with particle swarm optimization for biomarker selection...
  16. pmc LC-MS3 quantification of O-glycopeptides in human serum
    Miloslav Sanda
    Department of Oncology, Georgetown University, Washington, DC 20057, USA
    Electrophoresis 34:2342-9. 2013
    ..The possibility to analyze serum samples directly represents a significant improvement of the quantitative glycopeptide workflows with the potential for use in clinical applications. ..
  17. pmc Semi-automated identification of N-Glycopeptides by hydrophilic interaction chromatography, nano-reverse-phase LC-MS/MS, and glycan database search
    Petr Pompach
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, 3970 Reservoir Road NW, Washington, DC 20057 1465, United States
    J Proteome Res 11:1728-40. 2012
    ..The optimized workflow, coupled with GlycoPeptideSearch, is expected to make high-throughput semiautomated glycopeptide identification feasible for a wide range of users...
  18. pmc Acrolein-derived DNA adduct formation in human colon cancer cells: its role in apoptosis induction by docosahexaenoic acid
    Jishen Pan
    Lombardi Comprehensive Cancer Center, Georgetown University, 3800 Reservoir Road NW, Washington, DC 20057, USA
    Chem Res Toxicol 22:798-806. 2009
    ..Taken together, these results demonstrate the parallel relationships between Acr-dG level and apoptosis in HT-29 cells, suggesting that the formation of Acr-dG in cellular DNA may contribute to apoptosis induced by DHA...
  19. ncbi request reprint Quantification of DNA repair capacity in whole blood of patients with head and neck cancer and healthy donors by comet assay
    Daniel T Saha
    Georgetown University, Department of Oncology, Washington, DC 20057, USA
    Mutat Res 650:55-62. 2008
    ..Additional method optimization would be helpful to improve the assay for a large population screening...
  20. pmc Utilization of metabolomics to identify serum biomarkers for hepatocellular carcinoma in patients with liver cirrhosis
    Habtom W Ressom
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Anal Chim Acta 743:90-100. 2012
    ..Our work shows that metabolomic profiling is a promising tool to identify candidate metabolic biomarkers for early detection of HCC cases in high risk population of cirrhotic patients...
  21. pmc Site-specific glycan microheterogeneity of inter-alpha-trypsin inhibitor heavy chain h4
    Kevin Brown Chandler
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University, Washington, D C 20057, United States
    J Proteome Res 13:3314-29. 2014
    ..We also identified isoform-specific ITIH4 O-glycoforms and documented that utilization of O-glycosylation sites on ITIH4 differed between the cell line and serum. ..
  22. doi request reprint Analysis of peptides by denaturing ultrafiltration and LC-MALDI-TOF-MS
    Yanming An
    Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC, USA
    Methods Mol Biol 1023:13-9. 2013
    ..Here we describe coupling of denaturing ultrafiltration, an alternative depletion strategy, with reverse-phase fractionation and mass spectrometry for characterization of low-molecular-weight proteins and peptides...
  23. pmc Glycoprotein disease markers and single protein-omics
    Kevin Chandler
    Department of Biochemistry and Molecular and Cellular Biology, Georgetown University, Washington, DC 20057, USA
    Mol Cell Proteomics 12:836-45. 2013
    ..A new generation of targeted quantitative assays is expected to advance the use of glycoproteins in early detection of diseases, molecular disease classification, and monitoring of therapeutic interventions...
  24. pmc Proteome-wide analysis of single-nucleotide variations in the N-glycosylation sequon of human genes
    Raja Mazumder
    Department of Biochemistry and Molecular Biology, George Washington University Medical Center, Washington, DC, USA
    PLoS ONE 7:e36212. 2012
    ....
  25. pmc Urinary estrogen metabolites and prostate cancer risk: a pilot study
    Ourania Kosti
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, District of Columbia 20057, USA
    Prostate 71:507-16. 2011
    ..The aim of this pilot study was to evaluate urinary estrogen metabolites as a biomarker of prostate cancer risk...
  26. pmc Associations between cigarette smoking and mitochondrial DNA abnormalities in buccal cells
    Duanjun Tan
    Cancer Genetics and Epidemiology Program, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
    Carcinogenesis 29:1170-7. 2008
    ..Additional studies are needed to determine if mitochondrial mutation assays provide new or complementary information for estimating cigarette smoke exposure at the cellular level or as a cancer risk biomarker...
  27. ncbi request reprint The role of protein binding in induction of apoptosis by phenethyl isothiocyanate and sulforaphane in human non-small lung cancer cells
    Lixin Mi
    Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, District of Columbia 20057, USA
    Cancer Res 67:6409-16. 2007
    ..Because PEITC is a stronger inducer of apoptosis than SFN, these results indicate that direct covalent binding to cellular proteins is an important early event in the induction of apoptosis by the ITCs...
  28. pmc Structure-based comparative analysis and prediction of N-linked glycosylation sites in evolutionarily distant eukaryotes
    Phuc Vinh Nguyen Lam
    Life Sciences Department, Paris Diderot University, Paris 75013, France
    Genomics Proteomics Bioinformatics 11:96-104. 2013
    ..The tool, Structure Feature Analysis Tool (SFAT), is freely available to the public at http://hive.biochemistry.gwu.edu/tools/sfat...
  29. ncbi request reprint Food mutagens
    Radoslav Goldman
    Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC, USA
    J Nutr 133:965S-973S. 2003
    ..This article reviews the role of food mutagens in mutagenesis and carcinogenesis and how their effects are modified by heritable traits and discusses how to identify and evaluate the effects of food mutagens...
  30. ncbi request reprint A sensitive immunofluorescence assay for detection of p53 protein in the sputum
    Zumei Feng
    Department of Oncology, Lombardi Cancer Center, Georgetown University Medical Center, Washington, DC, USA
    Methods Mol Med 75:389-95. 2003

Research Grants8

  1. Glycans in Hepatocellular Carcinoma
    Radoslav Goldman; Fiscal Year: 2009
    ..The identified sugars could be used to screen high risk populations for early signs of liver cancer; to design and test new chemoprevention strategies; and to follow disease progression after treatment. ..
  2. Effects of smoking on ERb and AHR pathway in human lung
    Radoslav Goldman; Fiscal Year: 2004
    ..The grant would provide critical support for this pilot study, whose results will lead to refined hypotheses testable in future research. ..
  3. Proteomic Analysis of Serum in Liver Cancer
    Radoslav Goldman; Fiscal Year: 2010
    ..Identification of the P/P is expected to generate new hypotheses for targeted disease management. ..
  4. Proteomic Analysis of Serum in Liver Cancer
    Radoslav Goldman; Fiscal Year: 2007
    ..Identification of the P/P is expected to generate new hypotheses for targeted disease management. ..
  5. Optimization of enrichment for MALDI TOF/TOF identification of cancer biomarkers
    Radoslav Goldman; Fiscal Year: 2007
    ..Identification of the P/P is expected to generate new hypotheses for targeted disease management. ..
  6. Proteomic Analysis of Serum in Liver Cancer
    Radoslav Goldman; Fiscal Year: 2009
    ..Identification of the P/P is expected to generate new hypotheses for targeted disease management. ..
  7. Glycans in Hepatocellular Carcinoma
    Radoslav Goldman; Fiscal Year: 2010
    ..The identified sugars could be used to screen high risk populations for early signs of liver cancer;to design and test new chemoprevention strategies;and to follow disease progression after treatment. ..