Research Topics
| Sanjay AdhikariSummaryAffiliation: Georgetown University Country: USA Publications
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Detail Information
Publications
Dipole-dipole interaction stabilizes the transition state of apurinic/apyrimidinic endonuclease--abasic site interactionSanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, 3800 Reservoir Road NW, Washington, D C 20057, USA
J Biol Chem 283:1334-9. 2008..Thus, low activation energy and the enthalpy of activation, which are perhaps a result of dipole-dipole interactions, play critical roles in AP site binding of APE...
Evidence of complete cellular repair of 1,N6-ethenoadenine, a mutagenic and potential damage for human cancer, revealed by a novel methodSujata Choudhury
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
Mol Cell Biochem 313:19-28. 2008..Furthermore, the epsilonA repair in vivo and in vitro is predominant in the G0/G1 phase of the cell cycle...
Characterization of magnesium requirement of human 5'-tyrosyl DNA phosphodiesterase mediated reactionSanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
BMC Res Notes 5:134. 2012..Although hTDP2 possesses both 3'- and 5'- tyrosyl DNA phosphodiesterase activity, the role of Mg2+ in its activity was not studied in sufficient details...- Development of a novel assay for human tyrosyl DNA phosphodiesterase 2Sanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
Anal Biochem 416:112-6. 2011..Our results suggest that this novel assay system with this new TDP2 substrate can be used for inhibitor screening in a high-throughput manner... - A unified method for purification of basic proteinsSanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
Anal Biochem 400:203-6. 2010..Approximately, two-fifths of human proteome, including many if not all nucleic acid-interacting proteins, have a pI of 7.94 or higher; virtually all these 12,000 proteins may be purified using this method in a single step... - Excised damaged base determines the turnover of human N-methylpurine-DNA glycosylaseSanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, United States
DNA Repair (Amst) 8:1201-6. 2009..Thus, the results provide the first evidence that the excised base rather than AP-site could be rate-limiting for DNA-glycosylase reactions... - N-terminal extension of N-methylpurine DNA glycosylase is required for turnover in hypoxanthine excision reactionSanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, D C 20057, USA
J Biol Chem 282:30078-84. 2007..The results from this study also affirm the need for reinvestigation of full-length MPG for its enzymatic and structural properties, which are currently available mostly for the truncated protein... - Expression, purification and characterization of codon-optimized human N-methylpurine-DNA glycosylase from Escherichia coliSanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, LL level, S 122, 3800 Reservoir Road, NW, Washington, DC 20057, USA
Protein Expr Purif 58:257-62. 2008..Thus, this study indicates that this improved expression and purification system will facilitate large scale production and purification of a stable human MPG protein for further biochemical, biophysical and structure-function analysis... - A comparative study of recombinant mouse and human apurinic/apyrimidinic endonucleaseSanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
Mol Cell Biochem 362:195-201. 2012..This includes the development and validation of effective APE1 inhibitors as chemosensitizers in clinical studies... - Targeting base excision repair for chemosensitizationSanjay Adhikari
Lombardi Comprehensive Cancer Center, 3800 Reservoir Road NW, Georgetown University Medical Center, Washington, DC 20057, USA
Anticancer Agents Med Chem 8:351-7. 2008..Thus, MPG and other BER proteins could be potential targets for chemosensitization... - Suppression of tumor suppressor Tsc2 and DNA repair glycosylase Nth1 during spontaneous liver tumorigenesis in Long-Evans Cinnamon ratsShyama Prasad Sajankila
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical School, Washington, DC 20057, USA
Mol Cell Biochem 338:233-9. 2010..Increase in protein oxidation (carbonyl content) during the same time period (16-24 weeks) may have an effect on the promoter binding of regulatory proteins and consequent decrease in Nth1 and Tsc2 gene expressions during tumorigenesis... - Magnesium, essential for base excision repair enzymes, inhibits substrate binding of N-methylpurine-DNA glycosylaseSanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, USA
J Biol Chem 281:29525-32. 2006.... - Discrimination of lesion removal of N-methylpurine-DNA glycosylase revealed by a potent neutralizing monoclonal antibodySanjay Adhikari
Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC 20057, United States
DNA Repair (Amst) 7:31-9. 2008..Since we found that this antibody has an epitope in the N-terminal tail, the latter appears to have an important role in substrate discrimination, however, with a differential effect on different substrates...