Research Topics
Genomes and GenesSpecies | STEPHEN TAPSCOTTSummaryAffiliation: Fred Hutchinson Cancer Research Center Country: USA Publications
Research Grants
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Detail Information
Publications
miR-206 integrates multiple components of differentiation pathways to control the transition from growth to differentiation in rhabdomyosarcoma cellsKyle L MacQuarrie
Human Biology Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, C3 168, Seattle, WA, 98109, USA
Skelet Muscle 2:7. 2012..abstract:..- Inhibition of CD26/DPP-IV enhances donor muscle cell engraftment and stimulates sustained donor cell proliferationMaura H Parker
Program in Transplantation Biology, Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, Mailstop D1 100, Seattle, WA, 98109 1024, USA
Skelet Muscle 2:4. 2012..abstract:.. 
Fragile-X syndrome and myotonic dystrophy: parallels and paradoxesS J Tapscott
Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Curr Opin Genet Dev 8:245-53. 1998..At both loci, expansion is associated with altered chromatin, aberrant methylation, and suppressed expression of the adjacent FMR1 and DMAHP genes, implicating epigenetic mediation of these genetic diseases...- Deconstructing myotonic dystrophyS J Tapscott
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Science 289:1701-2. 2000..In a Perspective, Tapscott explains how findings from a new mouse model of DM (Mankodi et al.) could solve this paradox... - Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promotersYi Cao
Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
EMBO J 25:502-11. 2006..Therefore, the role of Myog in mediating terminal differentiation is, in part, to enhance expression of a subset of genes previously initiated by Myod... - Pbx acts with Hand2 in early myocardial differentiationLisa Maves
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Dev Biol 333:409-18. 2009..Our findings demonstrate new roles for Pbx proteins in vertebrate cardiac development and also provide new insight into connections between the transcriptional regulation of skeletal and cardiac muscle differentiation programs... - Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiationLisa Maves
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Development 134:3371-82. 2007..Our results reveal that Pbx proteins modulate Myod activity to drive fast-muscle gene expression, thus showing that homeodomain proteins can direct bHLH proteins to establish a specific cell-type identity... - Genome-wide MyoD binding in skeletal muscle cells: a potential for broad cellular reprogrammingYi Cao
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Dev Cell 18:662-74. 2010..Therefore, in addition to regulating muscle gene expression, MyoD binds genome wide and has the ability to broadly alter the epigenome in myoblasts and myotubes... - Pbx marks genes for activation by MyoD indicating a role for a homeodomain protein in establishing myogenic potentialCharlotte A Berkes
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Mol Cell 14:465-77. 2004..This demonstrates a specific mechanism of targeting MyoD to loci in inactive chromatin and reveals a critical role of homeodomain proteins in marking specific genes for activation in the muscle lineage... - Promoter-specific regulation of MyoD binding and signal transduction cooperate to pattern gene expressionDonald A Bergstrom
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Mol Cell 9:587-600. 2002..The identification of distinct molecular mechanisms that regulate discrete subprograms of myogenesis should facilitate analyses of differentiation in normal development and disease... - Gene expression in Huntington's disease skeletal muscle: a potential biomarkerAndrew D Strand
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Hum Mol Genet 14:1863-76. 2005..Furthermore, an understanding of the molecular basis of muscle dysfunction in HD should provide insight into mechanisms involved in neuronal abnormalities and neurodegeneration... - Large DNA palindromes as a common form of structural chromosome aberrations in human cancersHisashi Tanaka
Division of Basic Sciences, Fred Hitchinson Cancer Research Center, Seattle, Washington, USA
Hum Cell 19:17-23. 2006..A subset of loci containing palindromes is associated with gene amplification in Colo320DM, indicating that the location of palindromes in the cancer genome serves as a structural platform that supports subsequent gene amplification... - RNA transcripts, miRNA-sized fragments and proteins produced from D4Z4 units: new candidates for the pathophysiology of facioscapulohumeral dystrophyLauren Snider
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Hum Mol Genet 18:2414-30. 2009..Together, we have identified new sense and anti-sense RNA transcripts, novel mRNAs and mi/siRNA-sized RNA fragments generated from the D4Z4 units that are new candidates for the pathophysiology of FSHD... - Emerging parallels in the generation and regeneration of skeletal muscleLauren Snider
Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
Cell 113:811-2. 2003..show that Wnt signaling induces myogenesis in adult muscle stem cells in a manner analogous to muscle induction in the somite... - Widespread and nonrandom distribution of DNA palindromes in cancer cells provides a structural platform for subsequent gene amplificationHisashi Tanaka
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109-1024, USA
Nat Genet 37:320-7. 2005..Genome-wide analysis of palindrome formation is a new approach to identify structural chromosome aberrations associated with cancer... - The circuitry of a master switch: Myod and the regulation of skeletal muscle gene transcriptionStephen J Tapscott
Division of Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA 98109, USA
Development 132:2685-95. 2005..These studies are beginning to merge our understanding of how lineage-specific information is encoded in chromatin with how master regulatory factors drive programs of cell differentiation... - XIC is required for Siamois activity and dorsoanterior developmentLauren Snider
Fred Hutchinson Cancer Research Center, Seattle, WA 98109 1024, USA
Mol Cell Biol 25:5061-72. 2005..The data indicate a role for XIC in limiting Tcf3-dependent repression of Siamois activities that are required for goosecoid transcription and for dorsal organizer formation... - Intrastrand annealing leads to the formation of a large DNA palindrome and determines the boundaries of genomic amplification in human cancerHisashi Tanaka
Fred Hutchinson Cancer Reserach Center, Seattle, WA 98109, USA
Mol Cell Biol 27:1993-2002. 2007..Therefore, an early step of gene amplification is a regulated process that is facilitated by DNA inverted repeats in the genome... - Cell-lineage regulated myogenesis for dystrophin replacement: a novel therapeutic approach for treatment of muscular dystrophyEn Kimura
Department of Neurology, University of Washington School of Medicine, Seattle, WA 98195 7720, USA
Hum Mol Genet 17:2507-17. 2008..Our study provides a proof of concept for a novel gene/stem cell therapy technique and opens another potential therapeutic approach for degenerative muscle disorders... - A MyoD-generated feed-forward circuit temporally patterns gene expression during skeletal muscle differentiationBennett H Penn
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Genes Dev 18:2348-53. 2004.... - Hematopoietic stem cell transplantation does not restore dystrophin expression in Duchenne muscular dystrophy dogsChiara Dell'agnola
Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, D1 100, PO Box 19024, Seattle, WA 98109 1024, USA
Blood 104:4311-8. 2004..1% donor contribution... - MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206Miriam I Rosenberg
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
J Cell Biol 175:77-85. 2006..These findings demonstrate that MyoD, in addition to activating muscle-specific genes, induces miRNAs that repress gene expression during skeletal muscle differentiation... - BMP-2 mediates retinoid-induced apoptosis in medulloblastoma cells through a paracrine effectAndrew R Hallahan
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Nat Med 9:1033-8. 2003.... - DNA methylation of developmental genes in pediatric medulloblastomas identified by denaturation analysis of methylation differencesScott J Diede
Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Proc Natl Acad Sci U S A 107:234-9. 2010..This finding warrants strong consideration for DNA demethylating agents in future clinical trials for children with this disease... - Networking the nucleusIndika Rajapakse
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Mol Syst Biol 6:395. 2010..Studying the interaction within and among networks provides a useful framework for investigating the complex organization and dynamic function of the nucleus... - RNA-binding protein Muscleblind-like 3 (MBNL3) disrupts myocyte enhancer factor 2 (Mef2) {beta}-exon splicingKyung Soon Lee
Department of Pharmacology, University of Washington, Seattle, Washington 98195 2780, USA
J Biol Chem 285:33779-87. 2010..These studies suggest that elevating MBNL3 activity in myogenic cells could lead to muscle degeneration disorders such as myotonic dystrophy... - MyoD and E-protein heterodimers switch rhabdomyosarcoma cells from an arrested myoblast phase to a differentiated stateZhihong Yang
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Genes Dev 23:694-707. 2009.... - Sustained AAV-mediated dystrophin expression in a canine model of Duchenne muscular dystrophy with a brief course of immunosuppressionZejing Wang
Program in Transplantation Biology, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Mol Ther 15:1160-6. 2007..This protocol has potential applications to human clinical trials to enhance AAV-mediated therapies... - Loss of cell polarity causes severe brain dysplasia in Lgl1 knockout miceOlga Klezovitch
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Genes Dev 18:559-71. 2004.... - CTCF cis-regulates trinucleotide repeat instability in an epigenetic manner: a novel basis for mutational hot spot determinationRandell T Libby
Department of Laboratory Medicine, University of Washington Medical Center, Seattle, WA, USA
PLoS Genet 4:e1000257. 2008.... - Short inverted repeats initiate gene amplification through the formation of a large DNA palindrome in mammalian cellsHisashi Tanaka
Division of Basic Sciences and Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA
Proc Natl Acad Sci U S A 99:8772-7. 2002..This finding suggests that short inverted repeats in the mammalian genome can have a critical role in the initiation of gene amplification. This specific mechanism may provide a novel target for cancer therapies... - Expression of human alpha1-antitrypsin in mice and dogs following AAV6 vector-mediated gene transfer to the lungsChristine L Halbert
Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109 1024, USA
Mol Ther 18:1165-72. 2010.... - Immune responses to AAV in canine muscle monitored by cellular assays and noninvasive imagingZejing Wang
Program in Transplantation Biology, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Mol Ther 18:617-24. 2010..These assays should be incorporated into future human clinical trials of AAV gene therapy to monitor immune responses... - Selective instability: maternal effort and the evolution of gene activation and deactivation ratesCarlo C Maley
Fred Hutchinson Cancer Research Center, PO Box 19024, Seattle, WA 98109 1024, USA
Artif Life 9:317-26. 2003.... - Regulation of neuroD2 expression in mouse brainChin Hsing Lin
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Dev Biol 265:234-45. 2004.... - In vitro transcription system delineates the distinct roles of the coactivators pCAF and p300 during MyoD/E47-dependent transactivationF Jeffrey Dilworth
Division of Human Biology, Fred Hutchinson Cancer Research Center, and Department of Pathology, University of Washington School of Medicine, 1100 Fairview Avenue North C3 168, P O Box 19024, Seattle, WA 98109 1024, USA
Proc Natl Acad Sci U S A 101:11593-8. 2004..Further dissection of this in vitro transcription system should be highly useful toward elucidating the mechanism by which coactivators facilitate differential gene expression by MyoD... - Gene therapy in large animal models of muscular dystrophyZejing Wang
Division of Clinical Research, Fred Hutchinson Cancer Research Center in Seattle, Washington 98109, USA
ILAR J 50:187-98. 2009..Because of the complex nature of these diseases, it may be necessary to combine multiple approaches to achieve a successful treatment... - Antisense transcription and heterochromatin at the DM1 CTG repeats are constrained by CTCFDiane H Cho
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
Mol Cell 20:483-9. 2005.... - Immunity to adeno-associated virus-mediated gene transfer in a random-bred canine model of Duchenne muscular dystrophyZejing Wang
Program in Transplantation Biology, Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
Hum Gene Ther 18:18-26. 2007..Our data indicate that AAV2 and AAV6 capsid proteins can elicit primary cellular immune responses when injected into the skeletal muscle of random-bred dogs, and suggest the possibility of cellular immunity to AAV vectors in humans... - MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complexIvana L de la Serna
University of Massachusetts Medical School, Department of Cell Biology, 55 Lake Avenue North, Worcester, MA 01655, USA
Mol Cell Biol 25:3997-4009. 2005.... - MyoD and the transcriptional control of myogenesisCharlotte A Berkes
Colorado College, Colorado Springs, CO 80903, USA
Semin Cell Dev Biol 16:585-95. 2005..This review highlights recent studies regarding the molecular mechanisms by which the muscle-specific myogenic bHLH proteins interact with other regulatory factors to coordinate gene expression in a controlled and ordered manner... - Identification of transcriptional targets for Six5: implication for the pathogenesis of myotonic dystrophy type 1Shigeru Sato
Department of Biology, Jichi Medical School, Minamikawachi, Tochigi 329 0498, Japan
Hum Mol Genet 11:1045-58. 2002..Our results not only identify Six5 as an activator that directs Igfbp5 expression but also suggest that reduced SIX5 expression in DM1 might contribute to specific aspects of the DM1 phenotype... - Reciprocal inhibition between Pax7 and muscle regulatory factors modulates myogenic cell fate determinationHugo C Olguin
Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309, USA
J Cell Biol 177:769-79. 2007..This could represent an additional mechanism for the control of satellite cell fate decisions resulting in proliferation, differentiation, and self-renewal, necessary for skeletal muscle maintenance and repair... - Expression profiling of FSHD muscle supports a defect in specific stages of myogenic differentiationSara T Winokur
Department of Biological Chemistry, 202 Sprague Hall, University of California, Irvine, CA 92697, USA
Hum Mol Genet 12:2895-907. 2003..Improper nuclear localization of 4qter is discussed as an alternative model for FSHD gene regulation and pathogenesis... - Dysregulation of gene expression in the R6/2 model of polyglutamine disease: parallel changes in muscle and brainRuth Luthi-Carter
Center for Aging, Genetics and Neurodegeneration, Massachusetts General Hospital, Charlestown, MA 02129-4404, USA
Hum Mol Genet 11:1911-26. 2002..The complete dataset is available at www.neumetrix.info... - Regulation of thalamocortical patterning and synaptic maturation by NeuroD2Gulayse Ince-Dunn
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
Neuron 49:683-95. 2006..These observations indicate that NeuroD2 plays a critical role in regulating synaptic maturation and the patterning of thalamocortical connections... - Essential role for Dicer during skeletal muscle developmentJason R O'Rourke
Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32610 0266, USA
Dev Biol 311:359-68. 2007..These observations demonstrate key roles for Dicer in skeletal muscle and implicate miRNAs as critical components required for embryonic myogenesis... - The RNA helicases p68/p72 and the noncoding RNA SRA are coregulators of MyoD and skeletal muscle differentiationGiuseppina Caretti
Muscle Gene Expression Group, Laboratory of Muscle Biology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20829, USA
Dev Cell 11:547-60. 2006..These findings reveal that p68/p72 play a critical role in promoting the assembly of proteins required for the formation of the transcription initiation complex and chromatin remodeling...
Research Grants
- LINEAGE DETERMINATION IN MUSCLESTEPHEN TAPSCOTT; Fiscal Year: 2007..abstract_text> ..
- MYOTONIC DYSTROPHY LOCUS CONTROLSTEPHEN TAPSCOTT; Fiscal Year: 2007..The health relatedness is that understanding the normal role of CTG/CAG repeats in the genome will give new insight into their role in generating tissue specific diseases. ..
- 2007 Myogenesis Gordon ConferenceSTEPHEN TAPSCOTT; Fiscal Year: 2007..abstract_text> ..
- LINEAGE DETERMINATION IN MUSCLESTEPHEN TAPSCOTT; Fiscal Year: 2009..The knowledge from these studies will identify new therapeutic targets and strategies for the development of novel, differentiation based therapies. ..
- PRECLINICAL GENE THERAPY STUDIES IN CANINE MUSCULAR DYSTROPHYSTEPHEN TAPSCOTT; Fiscal Year: 2009..better understanding of the immunogenicity of AAV and developing even better and less toxic immunosuppression regimens will increase the likelihood of achieving the goal of effective gene therapy for human Duchenne muscular dystrophy ..
- LINEAGE DETERMINATION IN MUSCLESTEPHEN TAPSCOTT; Fiscal Year: 2009..This will provide new insight into the network mechanisms that regulate transitional states in mammalian cell differentiation. ..
- D4Z4 Coding Transcripts and FSHDStephen J Tapscott; Fiscal Year: 2010..PUBLIC HEALTH RELEVANCE: The human health relatedness of this proposal is that it will identify possible molecular causes of FSHD and provide a basis for future therapeutic development. ..
- LINEAGE DETERMINATION IN MUSCLEStephen J Tapscott; Fiscal Year: 2010..The knowledge from these studies will identify new therapeutic targets and strategies for the development of novel, differentiation based therapies. ..
- PRECLINICAL GENE THERAPY STUDIES IN CANINE MUSCULAR DYSTROPHYStephen J Tapscott; Fiscal Year: 2010..better understanding of the immunogenicity of AAV and developing even better and less toxic immunosuppression regimens will increase the likelihood of achieving the goal of effective gene therapy for human Duchenne muscular dystrophy ..
- Gordon Research Conference on MyogenesisSTEPHEN TAPSCOTT; Fiscal Year: 2004..abstract_text> ..
- NEUROD AND NEUROGENESISSTEPHEN TAPSCOTT; Fiscal Year: 2001..Th health relatedness of this work is that the ability to generate neurons and neuroendocrine cells will be critical for the treatment of numerous human diseases. ..
- LINEAGE DETERMINATION IN MUSCLESTEPHEN TAPSCOTT; Fiscal Year: 2002....
- MYOTONIC DYSTROPHY LOCUS CONTROLSTEPHEN TAPSCOTT; Fiscal Year: 2002....
- PRECLINICAL GENE THERAPY STUDIES IN CANINE MUSCULAR DYSTROPHYStephen J Tapscott; Fiscal Year: 2010..Reducing the immunogenicity of AAV vectors and developing better and less toxic immunosuppression regimens will increase the likelihood of achieving the goal of effective gene therapy for human DMD. ..